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https://www.readbyqxmd.com/read/28912828/dendritic-immunotherapy-improvement-for-an-optimal-control-murine-model
#1
J C Rangel-Reyes, J C Chimal-Eguía, E Castillo-Montiel
Therapeutic protocols in immunotherapy are usually proposed following the intuition and experience of the therapist. In order to deduce such protocols mathematical modeling, optimal control and simulations are used instead of the therapist's experience. Clinical efficacy of dendritic cell (DC) vaccines to cancer treatment is still unclear, since dendritic cells face several obstacles in the host environment, such as immunosuppression and poor transference to the lymph nodes reducing the vaccine effect. In view of that, we have created a mathematical murine model to measure the effects of dendritic cell injections admitting such obstacles...
2017: Computational and Mathematical Methods in Medicine
https://www.readbyqxmd.com/read/28910387/epstein-barr-virus-latent-membrane-protein-1-enhances-dendritic-cell-therapy-lymph-node-migration-activation-and-il-12-secretion
#2
James M Termini, Sachin Gupta, Francesca N Raffa, Elizabeth Guirado, Margaret A Fischl, Liguo Niu, Saravana Kanagavelu, Geoffrey W Stone
Dendritic cells (DC) are a promising cell type for cancer vaccines due to their high immunostimulatory capacity. However, improper maturation of DC prior to treatment may account for the limited efficacy of DC vaccine clinical trials. Latent Membrane Protein-1 (LMP1) of Epstein-Barr virus was examined for its ability to mature and activate DC as a gene-based molecular adjuvant for DC vaccines. DC were transduced with an adenovirus 5 vector (Ad5) expressing LMP1 under the control of a Tet-inducible promoter...
2017: PloS One
https://www.readbyqxmd.com/read/28887841/evidence-for-a-contributory-role-of-a-xenogeneic-immune-response-in-experimental-epidermolysis-bullosa-acquisita
#3
Markus Niebuhr, Michael Kasperkiewicz, Sebastian Maass, Eva Hauenschild, Katja Bieber, Ralf J Ludwig, Jürgen Westermann, Kathrin Kalies
Autoimmune diseases affect a large fraction of the population in Western countries. To elucidate the underlying causes, autoantibody transfer-induced mouse models have been established that greatly contributed to the understanding of the pathophysiology of these diseases. However, the role of a potentially co-occurring murine xenogeneic immune response to commonly utilized rabbit anti-mouse IgG remains poorly understood. Using the established rabbit anti-mouse type VII collagen (COL7) IgG-induced mouse model of the mucocutaneous blistering disorder epidermolysis bullosa acquisita (EBA), we found in this study a profound T and B cell response along with an altered cytokine expression profile in draining lymph nodes of mice injected with the xenogeneic IgG...
September 8, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28881247/time-trends-2006-2015-of-quality-indicators-in-eusoma-certified-breast-centres
#4
P A van Dam, M Tomatis, L Marotti, J Heil, R E Mansel, M Rosselli Del Turco, P J van Dam, D Casella, L G Bassani, M Danei, A Denk, D Egle, G Emons, K Friedrichs, N Harbeck, M Kiechle, R Kimmig, U Koehler, S Kuemmel, N Maass, C Mayr, A Prové, C Rageth, L Regolo, F Lorenz-Salehi, D Sarlos, C Singer, C Sohn, G Staelens, C Tinterri, R Audisio, A Ponti
AIM OF THE STUDY: The European Society of Breast Cancer Specialists (EUSOMA) has fostered a voluntary certification process for breast centres to establish minimum standards and ensure specialist multidisciplinary care. Prospectively collected anonymous information on primary breast cancer cases diagnosed and treated in the units is transferred annually to a central EUSOMA data warehouse for continuous monitoring of quality indicators (QIs) to improve quality of care. Units have to comply with the EUSOMA Breast Centre guidelines and are audited by peers...
September 4, 2017: European Journal of Cancer
https://www.readbyqxmd.com/read/28881081/a-genetically-inducible-porcine-model-of-intestinal-cancer
#5
Morten Møbjerg Callesen, Sigrid Salling Árnadóttir, Iben Lyskjaer, Mai-Britt Worm Ørntoft, Søren Høyer, Frederik Dagnaes-Hansen, Ying Liu, Rong Li, Henrik Callesen, Mads Heilskov Rasmussen, Martin Fogtmann Berthelsen, Martin Kristian Thomsen, Pawel Jan Schweiger, Kim Bak Jensen, Søren Laurberg, Torben Falck Ørntoft, Jannik Ejnar Elverløv-Jakobsen, Claus Lindbjerg Andersen
Transgenic porcine cancer models bring novel possibilities for research. Their physical similarities with humans enable the use of surgical procedures and treatment approaches used for patients, which facilitates clinical translation. Here, we aimed to develop an inducible oncopig model of intestinal cancer. Transgenic (TG) minipigs were generated using somatic cell nuclear transfer by hand-made cloning. The pigs encode two TG cassettes: 1) An Flp-recombinase inducible oncogene cassette containing KRAS-G12D, cMYC, SV40LT - which inhibits p53 - and pRB...
September 7, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28877360/improvement-of-the-efficacy-of-vascularized-lymph-node-transfer-for-lower-extremity-lymphedema-via-a-prefabricated-lympho-venous-shunt-through-lymphaticovenular-anastomosis-between-the-efferent-lymphatic-vessel-and-small-vein-in-the-elevated-vascularized-lymph
#6
Shinsuke Akita, Yoshihisa Yamaji, Hideki Tokumoto, Yoshitaro Sasahara, Yoshitaka Kubota, Motone Kuriyama, Nobuyuki Mitsukawa
BACKGROUND: Following vascularized lymph node (VLN) transfer (VLNT), the VLN may be at a risk for sclerosis because of efferent lymphatic vessel obstruction. We developed a new technique to prevent VLN sclerosis via a prefabricated lympho-venous (LV) shunt. This study compared the treatment outcomes of single VLNT with prefabricated LV shunt, conventional multiple VLNTs, and conventional single VLNT. METHODS: Overall, 47 limbs of 45 patients that underwent VLNT for lower-extremity lymphedema (LEL) in late clinical stage II/III were divided into 3 groups: conventional single VLNT group (control; 21 limbs where 4 had primary LEL), multiple VLNTs group (13 limbs where 3 had primary LEL), and prefabricated LV shunt group (13 limbs where 4 had primary LEL)...
September 6, 2017: Microsurgery
https://www.readbyqxmd.com/read/28872666/active-dissemination-of-cellular-antigens-by-dcs-facilitates-cd8-t-cell-priming-in-lymph-nodes
#7
Irina Gurevich, Tali Feferman, Idan Milo, Orna Tal, Ofra Golani, Ingo Drexler, Guy Shakhar
Antigen (Ag)-specific activation of naïve T cells by migrating dendritic cells (DCs) is a highly efficient process, although the chances for their co-localization in lymph nodes (LNs) appear low. Ag presentation may be delegated from Ag-donor DCs to the abundant resident DCs, but the routes of Ag transfer and how it facilitates T-cell activation remain unclear. We visualized CD8(+) T cell-DC interactions to study the sites, routes and cells mediating Ag transfer in mice. In vitro, Ag transfer from isolated ovalbumin (OVA)(+) bone marrow (BM)-DCs triggered widespread arrest, Ca(2+) flux and CD69 upregulation in OT-I T cells contacting recipient DCs...
September 5, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28867485/mechanisms-of-action-of-cannabidiol-in-adoptively-transferred-experimental-autoimmune-encephalomyelitis
#8
Coral González-García, Irene Moreno Torres, Ruth García-Hernández, Lucía Campos-Ruíz, Luis Rodríguez Esparragoza, María José Coronado, Aranzazu García Grande, Antonio García-Merino, Antonio J Sánchez López
Cannabidiol (CBD) is one of the most important compounds in Cannabis sativa, lacks psychotropic effects, and possesses a high number of therapeutic properties including the amelioration of experimental autoimmune encephalomyelitis (EAE). The aim of this study was to analyse the relative efficacy of CBD in adoptively transferred EAE (at-EAE), a model that allows better delineation of the effector phase of EAE. Splenocytes and lymph nodes from mice with actively induced EAE were cultured in the presence of MOG35-55 and IL-12 and inoculated intraperitoneally in recipient female C57BL/6J mice...
September 1, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28865053/dc-sign-promotes-japanese-encephalitis-virus-transmission-from-dendritic-cells-to-t-cells-via-virological-synapses
#9
Ping Wang, Mei Li, Wei Lu, Di Zhang, Qinxue Hu, Yalan Liu
Skin-resident dendritic cells (DCs) likely encounter incoming viruses in the first place, and their migration to lymph nodes following virus capture may promote viral replication. However, the molecular mechanisms underlying these processes remain unclear. In the present study, we found that compared to cell-free viruses, DC-bound viruses showed enhanced capture of JEV by T cells. Additionally, JEV infection was increased by co-culturing DCs and T cells. Blocking the C-type lectin receptor DC-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) with neutralizing antibodies or antagonists blocked JEV transmission to T cells...
August 31, 2017: Virologica Sinica
https://www.readbyqxmd.com/read/28855306/il-23-drives-differentiation-of-peripheral-%C3%AE-%C3%AE-17-t-cells-from-adult-bone-marrow-derived-precursors
#10
Pedro H Papotto, Natacha Gonçalves-Sousa, Nina Schmolka, Andrea Iseppon, Sofia Mensurado, Brigitta Stockinger, Julie C Ribot, Bruno Silva-Santos
Pro-inflammatory interleukin (IL)-17-producing γδ (γδ17) T cells are thought to develop exclusively in the thymus during fetal/perinatal life, as adult bone marrow precursors fail to generate γδ17 T cells under homeostatic conditions. Here, we employ a model of experimental autoimmune encephalomyelitis (EAE) in which hematopoiesis is reset by bone marrow transplantation and demonstrate unequivocally that Vγ4(+) γδ17 T cells can develop de novo in draining lymph nodes in response to innate stimuli...
August 30, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28845904/spreading-the-load-antigen-transfer-between-migratory-and-lymph-node-resident-dendritic-cells-promotes-t-cell-priming
#11
Scott N Mueller
Dendritic cells (DC) are specialized in the processing and presentation of antigen for the activation of lymphocytes. Multiple subsets of DCs exist with distinct functions and roles in the initiation of immune responses. DCs found within tissues acquire antigens or become infected by pathogens and migrate to local draining lymph nodes (LN) where they can directly stimulate T cells. These migratory DCs can also transfer antigens to LN-resident DCs and may indirectly enhance T cell priming. In this issue of the European Journal of Immunology, Gurevich et al...
August 28, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28831252/preparation-characterization-and-transfection-efficiency-of-low-molecular-weight-polyethylenimine-based-nanoparticles-for-delivery-of-the-plasmid-encoding-cd200-gene
#12
Fatemeh Nouri, Hossein Sadeghpour, Reza Heidari, Ali Dehshahri
Various strategies have been utilized to improve both gene transfer efficiency and cell-induced toxicity of polyethylenimine (PEI), the most extensively investigated cationic polymeric vector. In this study, we sought to enhance transfection efficiency of low molecular weight PEI (LMW PEI) while maintaining its low toxicity by cross-linking LMW PEI via succinic acid linker. These modifications were designed to improve the hydrophilic-hydrophobic balance of the polymer, by enhancing the buffering capacity and maintaining low cytotoxic effects of the final conjugate...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28820837/non-contrast-mr-lymphography-for-evaluation-of-lymph-node-transfer-for-secondary-upper-limb-lymphedema
#13
Lionel Arrivé, Sarah Derhy, Chrif Dlimi, Sanaa El Mouhadi, Laurence Monnier-Cholley, Corinne Becker
BACKGROUND: Our purpose was to evaluate the results of axillary lymph node transplantation (LNT) with non-contrast magnetic resonance (MR) lymphography in 15 patients with secondary upper limb lymphedema. METHODS: Fifteen female patients with lymphedema following breast cancer treatment underwent LNT. Non-contrast MR lymphography was obtained with a free-breathing 3D fast spin-echo sequence. Images analysis criteria included: (A) before surgery: a) severity of lymphedema graded as absent, mild, moderate, and severe; b) involvement of the muscular compartment; c) distal dilated lymphatic vessels; (B) after surgery: a) visualization of site of transplantation; b) visualization of transplanted lymph nodes; c) severity of lymphedema with regard to pretransplantation severity (i...
August 8, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28818700/vascularized-jejunal-mesenteric-lymph-node-transfer-a-novel-surgical-treatment-for-extremity-lymphedema
#14
Michelle Coriddi, Corrine Wee, Joseph Meyerson, Daniel Eiferman, Roman Skoracki
BACKGROUND: Vascularized lymph node transfer (VLNT) is a surgical treatment for lymphedema. Multiple donor sites have been described and each has significant disadvantages. We propose the jejunal mesentery as a novel donor site for VLNT. METHODS: Cadaveric anatomic study analyzing jejunal lymph nodes (LNs) and description of outcomes from the first patients who received jejunal mesenteric VLNT for treatment of lymphedema. RESULTS: In 5 cadavers, the average number of total LNs and peripheral LNs were identified in the proximal, middle and distal segments of jejunum...
August 14, 2017: Journal of the American College of Surgeons
https://www.readbyqxmd.com/read/28818619/lymph-node-targeting-strategies-to-improve-vaccination-efficacy
#15
Hao Jiang, Qin Wang, Xun Sun
With the rapid development of nanotechnology as well as growing interest in immunotherapy, a great number of vaccine delivery vehicles have been explored in order to elicit potent adaptive immune responses against various infections or tumors. Recent studies have shown that targeting vaccine to antigen-presenting cells (APCs) within lymph nodes is an effective strategy for improving antigen-specific adaptive immune response. However, the characteristics of vaccine vehicles, such as size, surface charge and the degree of PEGylation, affect lymph node transfer and subsequent APC uptake, leading to different levels of immune responses...
August 14, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28790247/-pulmonary-mucoepidermoid-carcinoma-with-hilar-lymph-node-metastasis-complicated-by-myelodysplastic-syndrome
#16
Yoshinari Matsuda, Hiroshi Moriyama, Masashi Inaba, Yoshihiko Kubo, Masahiro Kitada, Satoshi Hayashi, Kei Ishibashi, Satoshi Okazaki, Nana Takahashi, Shunsuke Yasuda, Masahiro Abe, Satoshi Hirata
A 65-year-old woman was found to have a mass shadow on chest computed tomography. The patient had been diagnosed as having myelodysplastic syndrome 3 years before admission. She was transferred to our hospital for further examination of the mass in the lower lobe of left lung. The mass was positron emission tomography-positive (SUVmax of 8.6)suggesting a malignant neoplasm. Serum concentrations of carcinoembryonic antigen was elevated to 8.7 ng/ml. Preoperative laboratory studies showed anemia (hemoglobin 6...
August 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28783695/tissue-specific-emergence-of-regulatory-and-intraepithelial-t-cells-from-a-clonal-t-cell-precursor
#17
Angelina M Bilate, Djenet Bousbaine, Luka Mesin, Marianna Agudelo, Justin Leube, Andreas Kratzert, Stephanie K Dougan, Gabriel D Victora, Hidde L Ploegh
Peripheral Foxp3(+) regulatory T cells (pTregs) maintain immune homeostasis by controlling potentially harmful effector T cell responses toward dietary and microbial antigens. Although the identity of the T cell receptor (TCR) can impose commitment and functional specialization of T cells, less is known about how TCR identity governs pTreg development from conventional CD4(+) T cells. To investigate the extent to which TCR identity dictates pTreg fate, we used somatic cell nuclear transfer to generate a transnuclear (TN) mouse carrying a monoclonal TCR from a pTreg (pTreg TN mice)...
August 26, 2016: Science Immunology
https://www.readbyqxmd.com/read/28783468/evaluation-of-experimental-infection-with-l-l-amazonensis-in-x-linked-immunodeficient-mice
#18
Patricia Xander, Wagner Francisco Kennerly Marcondes Gonzaga, Mariana Marques Geraldo, Bruno Camolese Vivanco, Ana Flavia Popi, Mario Mariano, Wagner Luiz Batista
B-1 cells are a subtype of B cells with peculiar characteristics. These cells are distinct from B-2 lymphocytes in their morphology, ontogeny, tissue distribution and phenotypic functional features. B-1 cells can participate in the immune response in several ways, for example, by being recruited to inflammatory foci, producing large amounts of IL-10 cytokine, and differentiating into IgM-secreting cells or phagocytes. Nevertheless, the role of B-1 cells in the pathogenesis of experimental leishmaniasis has not been fully elucidated...
August 7, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28767735/engaging-the-cd40-cd40l-pathway-augments-t-helper-cell-responses-and-improves-control-of-mycobacterium-tuberculosis-infection
#19
Jonathan Kevin Sia, Erica Bizzell, Ranjna Madan-Lala, Jyothi Rengarajan
Mycobacterium tuberculosis (Mtb) impairs dendritic cell (DC) functions and induces suboptimal antigen-specific CD4 T cell immune responses that are poorly protective. Mucosal T-helper cells producing IFN-γ (Th1) and IL-17 (Th17) are important for protecting against tuberculosis (TB), but the mechanisms by which DCs generate antigen-specific T-helper responses during Mtb infection are not well defined. We previously reported that Mtb impairs CD40 expression on DCs and restricts Th1 and Th17 responses. We now demonstrate that CD40-dependent costimulation is required to generate IL-17 responses to Mtb...
August 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28763794/targeting-latency-associated-peptide-promotes-antitumor-immunity
#20
Galina Gabriely, Andre P da Cunha, Rafael M Rezende, Brendan Kenyon, Asaf Madi, Tyler Vandeventer, Nathaniel Skillin, Stephen Rubino, Lucien Garo, Maria A Mazzola, Panagiota Kolypetri, Amanda J Lanser, Thais Moreira, Ana Maria C Faria, Hans Lassmann, Vijay Kuchroo, Gopal Murugaiyan, Howard L Weiner
Regulatory T cells (Tregs) promote cancer by suppressing antitumor immune responses. We found that anti-LAP antibody, which targets the latency-associated peptide (LAP)/transforming growth factor-β (TGF-β) complex on Tregs and other cells, enhances antitumor immune responses and reduces tumor growth in models of melanoma, colorectal carcinoma, and glioblastoma. Anti-LAP decreases LAP(+) Tregs, tolerogenic dendritic cells, and TGF-β secretion and is associated with CD8(+) T cell activation. Anti-LAP increases infiltration of tumors by cytotoxic CD8(+) T cells and reduces CD103(+) CD8 T cells in draining lymph nodes and the spleen...
May 19, 2017: Science Immunology
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