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Ann P Quick, Qiongling Wang, Leonne E Philippen, Giselle Barreto-Torres, David Y Chiang, David L Beavers, Guoliang Wang, Maha Khalid, Julia O Reynolds, Hannah M Campbell, Jordan Showell, Mark D McCauley, Arjen Scholten, Xander H Wehrens
RATIONALE: Junctional membrane complexes (JMC) in myocytes are critical microdomains, in which excitation-contraction coupling occurs. Structural and functional disruption of JMCs underlies contractile dysfunction in failing hearts. However, the role of newly identified JMC protein striated muscle preferentially expressed gene (SPEG) remains unclear. OBJECTIVE: To determine the role of SPEG in healthy and failing adult hearts. MMethods and Results: Proteomic analysis of immunoprecipatated JMC-proteins ryanodine receptor type-2 (RyR2) and junctophilin-2 (JPH2) followed by mass spectrometry identified the serine-threonine kinase SPEG as the only novel binding partner for both proteins...
October 11, 2016: Circulation Research
Punit Shah, Weiming Yang, Shisheng Sun, Jered Pasay, Nauder Faraday, Hui Zhang
Platelet glycoproteins are known to play central roles in hemostasis and vascular integrity and have pathologic roles in vascular occlusive diseases such as myocardial infarction and stroke. Characterizing glycoproteins within and secreted by platelets can provide insight into the mechanisms that underlie vascular pathologies and the therapeutic benefits or failure of anti-platelet agents. To study the impact of aspirin, which is commonly prescribed for primary and secondary cardiovascular prevention, on the platelet glycoproteome, we evaluated washed platelets from ten donors...
July 25, 2016: Proteomics
Ke-Chuan Wang, Chih-Hung Huang, Ching-Jou Huang, Shiuh-Bin Fang
Microfold or membranous (M) cells are specialized intestinal epithelial cells responsible for host immunity. The speG mutant of Salmonella Typhimurium (S. Typhimurium) is a nonreplicating strain within human cells to be a candidate vaccine vector for interacting with M cells. We conducted this study to identify the genes are differently expressed between in vitro M cells and Caco-2 cells, and to determine whether S. Typhimurium and speG affect the transcriptomes of both cell types. In vitro M cells and Caco-2 cells were infected with wild-type (WT) S...
2016: PloS One
Binghuai Lu, Yujie Fang, Lei Huang, Baowei Diao, Xiaoli Du, Biao Kan, Yanchao Cui, Fengxia Zhu, Dong Li, Duochun Wang
Streptococcus dysgalactiae subsp. equisimilis (SDSE) is presently considered as a human pathogen associated with clinical infection. We characterized 56 SDSE isolates collected from two tertiary hospitals in Beijing, China. Sixteen distinct emm types/subtypes were detected, dominated by stG245.0 (32.1%), stG652.0 (10.7%), stG6.1 (10.7%) and stG485.0 (10.7%), and a novel stG840.0 variant type was identified. All isolates possessed virulence genes of sagA and scpA, and most carried slo (98.2%), ska (98.2%) and speG(dys) (35...
June 2016: Infection, Genetics and Evolution
E Golińska, M van der Linden, G Więcek, D Mikołajczyk, A Machul, A Samet, A Piórkowska, M Dorycka, P B Heczko, M Strus
Invasive group A streptococcal (GAS) infections constitute an important epidemiological problem. Many cases occur in women during the postnatal period. The objective of this study was to evaluate the presence of the genes responsible for production of iron-chelating protein (perR) and superantigens (speA, speB, speC, speF, speG, speH, speI, speJ, speK, speL, speM, smeZ, and ssa) in S. pyogenes strains isolated from invasive infections in women after delivery. Furthermore, this study sought to verify whether S...
May 2016: European Journal of Clinical Microbiology & Infectious Diseases
Guilan Lu, Daitao Zhang, Jiachen Zhao, Yimeng Liu, Jing Guo, Shuangsheng Wu, Li Zhang, Peng Yang, Quanyi Wang, Xiaomin Peng
OBJECTIVE: To investigate superantigen gene profiles of group A Streptococcus (GAS) isolated in Beijing pediatric patients in 2014, and to explore the the relationship between superantigen gene profiles with emm types, and GAS infections with diseases. METHODS: A total of 259 GAS strains were isolated from pediatric patients clinically who diagnosed with scarlet fever and pharyngitis from 36 hospitals in Beijing from May to July, 2014.The Superantigens genes of strains were performed by Real-time PCR (speA, speB, speC, speF, speG, speH, speI, speJ, speK, speL, speM, smeZ, ssa)...
November 2015: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
Steven Weigand, Ekaterina V Filippova, Olga Kiryukhina, Wayne F Anderson
Here we describe the treatment of the small-angle X-ray Scattering (SAXS) data used during SpeG quaternary structure study as part of the research article "Substrate induced allosteric change in the quaternary structure of the spermidine N-acetyltransferase SpeG" published in Journal of Molecular Biology [1]. These data were collected on two separate area detectors as separate dilution series of the SpeG and the SpeG with spermine samples along with data from their companion buffers. The data were radially integrated, corrected for incident beam variation, and scaled to absolute units...
March 2016: Data in Brief
Hao He, Yingling Zhu, Liqing Chi, Zizhao Zhao, Ting Wang, Mingxing Zuo, Tong Zhang, Fengjuan Zhou, Liqiu Xia, Xuezhi Ding
OBJECTIVE: We isolated 339 bacillus strains from 72 soil samples all over the country, then purified their antimicrobial compounds and studied the antibacterial activity, to enrich bacillus resources and explore their second metabolites. METHODS: A bacillus strain with strong antibacterial activity was selected by dilution plate and water bath heating from a soil sample from a peanut plantation in Henan Province; this strain was identified according to morphological observation, physiological and biochemical characteristics, and consequences of 16S rRNA homologous analysis...
September 4, 2015: Wei Sheng Wu Xue Bao, Acta Microbiologica Sinica
Xiaoli Liu, Sean R R Hall, Zhihong Wang, He Huang, Sailaja Ghanta, Moises Di Sante, Annarosa Leri, Piero Anversa, Mark A Perrella
Striated preferentially expressed gene (Speg) is a member of the myosin light chain kinase family. We previously showed that disruption of the Speg gene locus in mice leads to a dilated cardiomyopathy with immature-appearing cardiomyocytes. Here we show that cardiomyopathy of Speg(-/-) mice arises as a consequence of defects in cardiac progenitor cell (CPC) function, and that neonatal cardiac dysfunction can be rescued by in utero injections of wild-type CPCs into Speg(-/-) foetal hearts. CPCs harvested from Speg(-/-) mice display defects in clone formation, growth and differentiation into cardiomyocytes in vitro, which are associated with cardiac dysfunction in vivo...
November 23, 2015: Nature Communications
Eva Leitner, Ines Zollner-Schwetz, Gernot Zarfel, Lilian Masoud-Landgraf, Michael Gehrer, Ute Wagner-Eibel, Andrea J Grisold, Gebhard Feierl
OBJECTIVES: An increase of severe infections caused by Streptococcus dysgalactiae subsp. equisimilis (SDSE) similar to infections caused by Streptococcus pyogenes has been reported over the last years. Little is known about infections with SDSE in Austria. Therefore, we investigated a collection of 113 SDSE invasive and non-invasive isolates from different infection sites and type of infections as well as patients' characteristics. METHODS: The isolates were phenotypically identified and emm typed using the enlarged emm database from the Centers for Disease Control and Prevention...
December 2015: International Journal of Medical Microbiology: IJMM
Ekaterina V Filippova, Steven Weigand, Jerzy Osipiuk, Olga Kiryukhina, Andrzej Joachimiak, Wayne F Anderson
The spermidine N-acetyltransferase SpeG is a dodecameric enzyme that catalyzes the transfer of an acetyl group from acetyl coenzyme A to polyamines such as spermidine and spermine. SpeG has an allosteric polyamine-binding site and acetylating polyamines regulate their intracellular concentrations. The structures of SpeG from Vibrio cholerae in complexes with polyamines and cofactor have been characterized earlier. Here, we present the dodecameric structure of SpeG from V. cholerae in a ligand-free form in three different conformational states: open, intermediate and closed...
November 6, 2015: Journal of Molecular Biology
Suvendu Mondal, A M Badigannavar
The peanut rust disease is an economically important biotic stress that significantly reduces the pod and fodder yield and oil quality. It is caused by the basidiomycete fungus Puccinia arachidis Speg. which belongs to class Pucciniomycetes like other rust fungus but has fewer occurrences in teliospore form. The P. arachidis predominantly spreads by the repeated cycle of uredospores in the field. The disease is prevalent in most of the countries where peanut is cultivated and favored by warm and humid climatic conditions...
November 2015: Protoplasma
Zhenyan Liu, Ming Fang, Bin Hu, Zhenwang Bi, Zengqiang Kou, Yanyan Ren, Baoli Chen, Zhenqiang Bi
OBJECTIVE: To describe the molecular characteristics of group A Streptococcus (GAS) isolated from patients and asymptomatic carriers of scarlet fever in Shandong province, 2013, and to explore the relationships between emm types and other molecular types. METHODS: 72 strains of GAS were isolated from throat swabs of children with scarlet fever or asymptomatic carriers of GAS. All the strains were typed by emm typing, multilocus sequence typing (MLST), super-antigen (SAg) genes detections and pulsed-field gel electrophoreses (PFGE)...
December 2014: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Ekaterina V Filippova, Misty L Kuhn, Jerzy Osipiuk, Olga Kiryukhina, Andrzej Joachimiak, Miguel A Ballicora, Wayne F Anderson
Spermidine N-acetyltransferase, encoded by the gene speG, catalyzes the initial step in the degradation of polyamines and is a critical enzyme for determining the polyamine concentrations in bacteria. In Escherichia coli, studies have shown that SpeG is the enzyme responsible for acetylating spermidine under stress conditions and for preventing spermidine toxicity. Not all bacteria contain speG, and many bacterial pathogens have developed strategies to either acquire or silence it for pathogenesis. Here, we present thorough kinetic analyses combined with structural characterization of the VCA0947 SpeG enzyme from the important human pathogen Vibrio cholerae...
March 27, 2015: Journal of Molecular Biology
Keqiang Wei, Shengxi Pang, Junxian Yang, Zhizhong Wei
The tobacco has the genetic potential to remove toxic metals from the soil. To develop hyperaccumulating tobacco plants, distant hybridization between tobacco (Nicotiana tabacum L.), a high-biomass crop, and Perilla frutescens (L.) Britt var. frutescens, a newfound Cd-hyperaccumulator species, was carried out using a novel method viz. pollination following grafting. Their hybrid nature was preliminarily confirmed by phenotype, isozyme pattern, random amplified polymorphic DNA (RAPD) and metabolites analysis...
April 2015: Environmental Science and Pollution Research International
Siroj Jitprasutwit, Catherine Ong, Niramol Juntawieng, Wen Fong Ooi, Claudia M Hemsley, Paiboon Vattanaviboon, Richard W Titball, Patrick Tan, Sunee Korbsrisate
BACKGROUND: Burkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative bacterium widely distributed in soil and water in endemic areas. This soil saprophyte can survive harsh environmental conditions, even in soils where herbicides (containing superoxide generators) are abundant. Sigma factor E (σE) is a key regulator of extra-cytoplasmic stress response in Gram-negative bacteria. In this study, we identified the B. pseudomallei σE regulon and characterized the indirect role that σE plays in the regulation of spermidine, contributing to the successful survival of B...
2014: BMC Genomics
Rosaria Campilongo, Maria Letizia Di Martino, Lucia Marcocci, Paola Pietrangeli, Adriano Leuzzi, Milena Grossi, Mariassunta Casalino, Mauro Nicoletti, Gioacchino Micheli, Bianca Colonna, Gianni Prosseda
Polyamines are small molecules associated with a wide variety of physiological functions. Bacterial pathogens have developed subtle strategies to exploit polyamines or manipulate polyamine-related processes to optimize fitness within the host. During the transition from its innocuous E. coli ancestor, Shigella, the aetiological agent of bacillary dysentery, has undergone drastic genomic rearrangements affecting the polyamine profile. A pathoadaptation process involving the speG gene and the cad operon has led to spermidine accumulation and loss of cadaverine...
2014: PloS One
Pankaj B Agrawal, Christopher R Pierson, Mugdha Joshi, Xiaoli Liu, Gianina Ravenscroft, Behzad Moghadaszadeh, Tiffany Talabere, Marissa Viola, Lindsay C Swanson, Göknur Haliloğlu, Beril Talim, Kyle S Yau, Richard J N Allcock, Nigel G Laing, Mark A Perrella, Alan H Beggs
Centronuclear myopathies (CNMs) are characterized by muscle weakness and increased numbers of central nuclei within myofibers. X-linked myotubular myopathy, the most common severe form of CNM, is caused by mutations in MTM1, encoding myotubularin (MTM1), a lipid phosphatase. To increase our understanding of MTM1 function, we conducted a yeast two-hybrid screen to identify MTM1-interacting proteins. Striated muscle preferentially expressed protein kinase (SPEG), the product of SPEG complex locus (SPEG), was identified as an MTM1-interacting protein, confirmed by immunoprecipitation and immunofluorescence studies...
August 7, 2014: American Journal of Human Genetics
Nathalie M Karaky, George F Araj, Sima T Tokajian
Streptococcus pyogenes [Group A Streptococcus (GAS)] is one of the most important human pathogens, responsible for numerous diseases with diverse clinical manifestations. As the epidemiology of GAS infections evolves, a rapid and reliable characterization of the isolates remains essential for epidemiological analysis and infection control. This study investigated the epidemiological patterns and genetic characteristics of 150 GAS isolates from a tertiary hospital in Lebanon by emm typing, superantigens (SAgs) detection, PFGE and antibiotic profiling...
September 2014: Journal of Medical Microbiology
Rajeev K Varshney, Manish K Pandey, Pasupuleti Janila, Shyam N Nigam, Harikishan Sudini, M V C Gowda, Manda Sriswathi, T Radhakrishnan, Surendra S Manohar, Patne Nagesh
Successful introgression of a major QTL for rust resistance, through marker-assisted backcrossing, in three popular Indian peanut cultivars generated several promising introgression lines with enhanced rust resistance and higher yield. Leaf rust, caused by Puccinia arachidis Speg, is one of the major devastating diseases in peanut (Arachis hypogaea L.). One QTL region on linkage group AhXV explaining upto 82.62 % phenotypic variation for rust resistance was validated and introgressed from cultivar 'GPBD 4' into three rust susceptible varieties ('ICGV 91114', 'JL 24' and 'TAG 24') through marker-assisted backcrossing (MABC)...
August 2014: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
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