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https://www.readbyqxmd.com/read/29217201/expression-and-purification-of-the-human-epidermal-growth-factor-receptor-extracellular-domain
#1
Linpeng Wang, Jing Yan, Jingyun Yan, Huanhuan Xu, Dengyang Zhang, Xuanjun Wang, Jun Sheng
In the fields of drug discovery and protein science, small quantities of proteins are always needed to investigate or validate protein-protein (or protein-small molecule) interactions. Traditional transient or stable expression method to obtain recombinant proteins in eukaryotic systems can be laborious and time-consuming, especially when multiple protein variants are required. Here, we present a fast and convenient method for obtaining small quantities of recombinant human epidermal growth factor receptor (rhEGFR) ectodomain protein, which could be efficiently extended to the expression of other eukaryotic proteins...
December 4, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29216310/gbm-auto-a-software-tool-to-simplify-spatial-modelling-and-marine-protected-area-planning
#2
Simon Dedman, Rick Officer, Maurice Clarke, David G Reid, Deirdre Brophy
BOOSTED REGRESSION TREES. EXCELLENT FOR DATA-POOR SPATIAL MANAGEMENT BUT HARD TO USE: Marine resource managers and scientists often advocate spatial approaches to manage data-poor species. Existing spatial prediction and management techniques are either insufficiently robust, struggle with sparse input data, or make suboptimal use of multiple explanatory variables. Boosted Regression Trees feature excellent performance and are well suited to modelling the distribution of data-limited species, but are extremely complicated and time-consuming to learn and use, hindering access for a wide potential user base and therefore limiting uptake and usage...
2017: PloS One
https://www.readbyqxmd.com/read/29210001/high-intensity-exercise-interventions-in-cancer-survivors-a-systematic-review-exploring-the-impact-on-health-outcomes
#3
REVIEW
Kellie Toohey, Kate Pumpa, Andrew McKune, Julie Cooke, Stuart Semple
PURPOSE: There is an increasing body of evidence underpinning high-intensity exercise as an effective and time-efficient intervention for improving health in cancer survivors. The aim of this study was to, (1) evaluate the efficacy and (2) the safety of high-intensity exercise interventions in improving selected health outcomes in cancer survivors. METHODS: Design Systematic review. Data sources Google Scholar and EBSCO, CINAHL Plus, Computers and Applied Sciences Complete, Health Source-Consumer Edition, Health Source: Nursing/Academic Edition, MEDLINE, Web of Science and SPORTDiscuss from inception up until August 2017...
December 5, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29209906/the-fourth-annual-brds-on-genome-editing-and-silencing-for-precision-medicines
#4
Amit Kumar Chaudhary, Rajan Sharma Bhattarai, Ram I Mahato
Precision medicine is promising for treating human diseases, as it focuses on tailoring drugs to a patient's genes, environment, and lifestyle. The need for personalized medicines has opened the doors for turning nucleic acids into therapeutics. Although gene therapy has the potential to treat and cure genetic and acquired diseases, it needs to overcome certain obstacles before creating the overall prescription drugs. Recent advancement in the life science has helped to understand the effective manipulation and delivery of genome-engineering tools better...
December 5, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29208034/optimal-database-combinations-for-literature-searches-in-systematic-reviews-a-prospective-exploratory-study
#5
Wichor M Bramer, Melissa L Rethlefsen, Jos Kleijnen, Oscar H Franco
BACKGROUND: Within systematic reviews, when searching for relevant references, it is advisable to use multiple databases. However, searching databases is laborious and time-consuming, as syntax of search strategies are database specific. We aimed to determine the optimal combination of databases needed to conduct efficient searches in systematic reviews and whether the current practice in published reviews is appropriate. While previous studies determined the coverage of databases, we analyzed the actual retrieval from the original searches for systematic reviews...
December 6, 2017: Systematic Reviews
https://www.readbyqxmd.com/read/29202919/stakeholders-guidance-document-for-consumer-analytical-devices-with-a-focus-on-gluten-and-food-allergens
#6
Bert Popping, Laura Allred, François Bourdichon, Kurt Brunner, Carmen Diaz-Amigo, Patricia Galan-Malo, Markus Lacorn, Jennifer North, Salvatore Parisi, Adrian Rogers, Jennifer Sealy-Voyksner, Tricia Thompson, Jupiter Yeung
Until recently, analytical tests for food were performed primarily in laboratories, but technical developmentsnow enable consumers to use devices to test their food at home or when dining out. Current consumer devices for food can determine nutritional values, freshness, and, most recently, the presence of food allergens and substances that cause food intolerances. The demand for such products is driven by an increase in the incidence of food allergies, as well as consumer desire for more information about what is in their food...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202911/the-allergen-bureau-vital-program
#7
Simon Brooke Taylor, Georgina Christensen, Kirsten Grinter, Robin Sherlock, Lisa Warren
This paper sets out the role of the Allergen Bureau and the Voluntary Incidental Trace Allergen Labelling (VITAL) Program from its origin in 2007 to its current iteration, VITAL 2. Herewith are outlined the scientific principles that support the program; the program's application in the food chain; and the benefits of the program's use to the food industry, clinicians, and the allergic consumer. VITAL was developed by the Australian and New Zealand food industry in consultation with multiple stakeholders, including consumer organizations, industry bodies, regulators, and retailers, to provide a standardized, science-based risk assessment process for the investigation of the potential presence of food allergens due to cross-contact and to determine whether, for cases in which the allergen is unable to be removed or controlled consistently, precautionary statements are required...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202907/challenges-and-path-forward-on-mandatory-allergen-labeling-and-voluntary-precautionary-allergen-labeling-for-a-global-company
#8
Jupiter Yeung, Marie-Claude Robert
For food manufacturers, the label on a food package is a tool meant to alert consumers to the presence of specific allergens, allowing consumers to make informed decisions and not unnecessarily limit their food choices. Mandatory allergen labeling is used when the allergen is an intentionally added ingredient, whereas voluntary allergen labeling is used when the presence of the allergen is unintentional and may be in the finished product as a result of cross-contact. In a globalized economy, ensuring food safety is a growing challenge for manufacturers...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202337/using-handwriting-to-infer-a-writer-s-country-of-origin-for-forensic-intelligence-purposes
#9
Anna Agius, Marie Morelato, Sébastien Moret, Scott Chadwick, Kylie Jones, Rochelle Epple, James Brown, Claude Roux
Forensic science has traditionally focused the majority of its resources and objectives towards addressing Court-related questions. However, this view restricts the contribution of forensic science to one process and results in a loss of information as the investigative and intelligence roles are largely neglected. A forensic science discipline suffering from this imbalance is handwriting examination, which may be characterised as a time consuming and subjective process that is mostly carried out towards the end of the investigation for the purpose of judicial proceedings...
November 24, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29200538/using-google-scholar-institutional-level-data-to-evaluate-the-quality-of-university-research
#10
John Mingers, Jesse R O'Hanley, Musbaudeen Okunola
In recent years, the extent of formal research evaluation, at all levels from the individual to the multiversity has increased dramatically. At the institutional level, there are world university rankings based on an ad hoc combination of different indicators. There are also national exercises, such as those in the UK and Australia that evaluate research outputs and environment through peer review panels. These are extremely costly and time consuming. This paper evaluates the possibility of using Google Scholar (GS) institutional level data to evaluate university research in a relatively automatic way...
2017: Scientometrics
https://www.readbyqxmd.com/read/29198107/fingerprint-analysis-moving-toward-multi-attribute-determination-via-individual-markers
#11
Erica Brunelle, Crystal Huynh, Eden Alin, Morgan Eldridge, Anh Minh Le, Lenka Halámková, Jan Halámek
Forensic science will be forever revolutionized if law enforcement can identify personal attributes of a person of interest solely from a fingerprint. For the past 2 years, the goal of our group was to establish a way to identify originator attributes, specifically biological sex, from a single analyte. To date, an enzymatic assay and two chemical assays have been developed for the analysis of multiple analytes. In this manuscript, two additional assays have been developed. This time, however, the assays utilize only one amino acid each...
December 2, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29190114/the-scientific-basis-for-healthful-carbohydrate-profile
#12
Lisa M Lamothe, Kim-Anne Lê, Rania Abou Samra, Olivier Roger, Hilary Green, Katherine Macé
Dietary guidelines indicate that complex carbohydrates should provide around half of the calories in a balanced diet, while sugars (i.e., simple carbohydrates) should be limited to no more than 5-10% of total energy intake. To achieve this public health goal a collective effort from different entities including governments, food & beverage industries and consumers is required. Some food companies have committed to continually reduce sugars in their products. Different solutions can be used to replace sugars in food products but it is important to ensure that these solutions are more healthful than the sugars they replace...
November 30, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29177916/direct-to-consumer-genetic-testing-law-and-policy-concerns-in-ireland
#13
Aisling de Paor
BACKGROUND: With rapid scientific and technological advances, the past few years has witnessed the emergence of a new genetic era and a growing understanding of the genetic make-up of human beings. These advances have propelled the introduction of companies offering direct to consumer (DTC) genetic testing, which facilitates the direct provision of such tests to consumers, (for example, via the internet). Although DTC genetic testing offers benefits by enhancing consumer accessibility to such technology, promoting proactive healthcare and increasing genetic awareness, it presents a myriad of challenges, from an ethical, legal and regulatory perspective...
November 25, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29170738/bim-sim-interactive-simulation-of-broadband-imaging-using-mie-theory
#14
Sebastian Berisha, Thomas van Dijk, Rohit Bhargava, P Scott Carney, David Mayerich
Understanding the structure of a scattered electromagnetic (EM) field is critical to improving the imaging process. Mechanisms such as diffraction, scattering, and interference affect an image, limiting the resolution, and potentially introducing artifacts. Simulation and visualization of scattered fields thus plays an important role in imaging science. However, EM fields are high-dimensional, making them time-consuming to simulate, and difficult to visualize. In this paper, we present a framework for interactively computing and visualizing EM fields scattered by micro and nano-particles...
2017: Frontiers in Physics
https://www.readbyqxmd.com/read/29170695/students-perceptions-of-an-applied-research-experience-in-an-undergraduate-exercise-science-course
#15
Regis C Pearson, K Jason Crandall, Kathryn Dispennette, Jill M Maples
Applied research experiences can provide numerous benefits to undergraduate students, however few studies have assessed the perceptions of Exercise Science (EXS) students to an applied research experience. The purpose of this study was two-fold: 1) to describe the rationale and implementation of an applied research experience into an EXS curriculum and 2) to evaluate EXS undergraduate students' perceptions of an applied research experience. An EXS measurement course was chosen for implementation of an applied research experience...
2017: International Journal of Exercise Science
https://www.readbyqxmd.com/read/29168569/point-of-use-fortification-of-foods-with-micronutrient-powders-containing-iron-in-children-of-preschool-and-school-age
#16
REVIEW
Luz Maria De-Regil, Maria Elena D Jefferds, Juan Pablo Peña-Rosas
BACKGROUND: Approximately 600 million children of preschool and school age are anaemic worldwide. It is estimated that at least half of the cases are due to iron deficiency. Point-of-use fortification of foods with micronutrient powders (MNP) has been proposed as a feasible intervention to prevent and treat anaemia. It refers to the addition of iron alone or in combination with other vitamins and minerals in powder form, to energy-containing foods (excluding beverages) at home or in any other place where meals are to be consumed...
November 23, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29165068/identifying-candidates-for-breast-cancer-using-interactions-of-chemicals-and-proteins
#17
Jing Lu, Kangle Shang, Yi Bi
Breast cancer is one of the major cause of cancer death in women worldwide. Therefore, it is urgent to discovery novel drugs or design effective treatments for this disease. However, the research and development of drugs by using only experimental methods is always time-consuming and expensive. With the development of computer science, some advanced computational methods can make full use of known knowledge to design candidate drugs, thereby reducing the cost and time of experimental testing. In this study, a computational method was proposed to identify novel candidates for breast cancer...
November 20, 2017: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/29159991/journey-to-the-centre-of-the-cell-virtual-reality-immersion-into-scientific-data
#18
REVIEW
Angus P R Johnston, James Rae, Nicholas Ariotti, Benjamin Bailey, Andrew Lija, Robyn Webb, Charles Ferguson, Sheryl Maher, Thomas P Davis, Richard I Webb, John McGhee, Robert G Parton
Visualization of scientific data is crucial not only for scientific discovery but also to communicate science and medicine to both experts and a general audience. Until recently, we have been limited to visualizing the three-dimensional (3D) world of biology in 2 dimensions. Renderings of 3D cells are still traditionally displayed using two-dimensional (2D) media, such as on a computer screen or paper. However, the advent of consumer grade virtual reality (VR) headsets such as Oculus Rift and HTC Vive means it is now possible to visualize and interact with scientific data in a 3D virtual world...
November 21, 2017: Traffic
https://www.readbyqxmd.com/read/29158043/six-years-after-the-nrc-review-of-epa-s-draft-iris-toxicological-review-of-formaldehyde-regulatory-implications-of-new-science-in-evaluating-formaldehyde-leukemogenicity
#19
Kenneth A Mundt, P Robinan Gentry, Linda D Dell, Joseph V Rodricks, Paolo Boffetta
Shortly after the International Agency for Research on Cancer (IARC) determined that formaldehyde causes leukemia, the United States Environmental Protection Agency (EPA) released its Draft IRIS Toxicological Review of Formaldehyde, also concluding that formaldehyde causes leukemia. Peer review of the EPA Draft IRIS Assessment by a National Academy of Science committee noted that "causal determinations are not supported by the narrative provided in the draft" {NRC 2011}. They offered recommendations for improving the IRIS review and identified several important research gaps...
November 17, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/29153178/a-100-year-review-the-production-of-fluid-market-milk
#20
David M Barbano
During the first 100 years of the Journal of Dairy Science, dairy foods and dairy production dairy scientists have partnered to publish new data and research results that have fostered the development of new knowledge. This knowledge has been the underpinning of both the commercial development of the fluid milk processing industry and regulations and marketing policies for the benefit of dairy farmers, processors, and consumers. During the first 50 years, most of the focus was on producing and delivering high-quality raw milk to factories and improving the shelf life of pasteurized fluid milk...
December 2017: Journal of Dairy Science
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