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https://www.readbyqxmd.com/read/29687387/three-dimensional-magnetic-levitation-culture-system-simulating-white-adipose-tissue
#1
Hubert Tseng, Alexes C Daquinag, Glauco R Souza, Mikhail G Kolonin
White adipose tissue (WAT) has attracted interest for tissue engineering and cell-based therapies as an abundant source of adipose stem/stromal cells (ASC). However, technical challenges in WAT cell culture have limited its applications in regenerative medicine. Traditional two-dimensional (2D) cell culture models, which are essentially monolayers of cells on glass or plastic substrates, inadequately represent tissue architecture, biochemical concentration gradients, substrate stiffness, and most importantly for WAT research, cell phenotypic heterogeneity...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29686876/an-exploration-of-values-among-consumers-seeking-treatment-for-borderline-personality-disorder
#2
Simone R Mohi, Frank P Deane, Anne Bailey, Dianne Mooney-Reh, Danielle Ciaglia
Background: Consumer feedback identifies a new challenge in the treatment of borderline personality disorder (BPD) is to address the discrepancy between clinical treatment targets and the more personally meaningful goals people are seeking in treatment. This highlights the need to increase clarification of people's values and link these to therapy goals. The current study explores ways in which individuals with BPD identify with values across key life domains. Methods: At initial assessment 106 consumer participants attending an outpatient clinic for the treatment of BPD completed the Personal Values Questionnaire by Blackledge and colleagues...
2018: Borderline Personality Disorder and Emotion Dysregulation
https://www.readbyqxmd.com/read/29675776/pluripotent-stem-cells-for-retinal-tissue-engineering-current-status-and-future-prospects
#3
REVIEW
Ratnesh Singh, Oscar Cuzzani, François Binette, Hal Sternberg, Michael D West, Igor O Nasonkin
The retina is a very fine and layered neural tissue, which vitally depends on the preservation of cells, structure, connectivity and vasculature to maintain vision. There is an urgent need to find technical and biological solutions to major challenges associated with functional replacement of retinal cells. The major unmet challenges include generating sufficient numbers of specific cell types, achieving functional integration of transplanted cells, especially photoreceptors, and surgical delivery of retinal cells or tissue without triggering immune responses, inflammation and/or remodeling...
April 19, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29603734/school-based-physical-therapy-services-and-student-functional-performance-at-school
#4
Sarah Westcott Mccoy, Susan K Effgen, Lisa A Chiarello, Lynn M Jeffries, Alejandro G Villasante Tezanos
AIM: We explored relationships of school-based physical therapy to standardized outcomes of students receiving physical therapy. METHOD: Using a practice-based evidence research design, School Function Assessment (SFA) outcomes of 296 students with disabilities (mean age 7y 4mo [standard deviation 2y]; 166 males, 130 females), served by 109 physical therapists, were explored. After training, therapists completed 10 SFA scales on students at the beginning and end of the school year...
March 30, 2018: Developmental Medicine and Child Neurology
https://www.readbyqxmd.com/read/29597317/contributions-of-bioactive-molecules-in-stem-cell-based-periodontal-regeneration
#5
REVIEW
An-Qi Liu, Cheng-Hu Hu, Fang Jin, Li-Shu Zhang, Kun Xuan
Periodontal disease is a widespread disease, which without proper treatment, may lead to tooth loss in adults. Because stem cells from the inflammatory microenvironment created by periodontal disease exhibit impaired regeneration potential even under favorable conditions, it is difficult to obtain satisfactory therapeutic outcomes using traditional treatments, which only focus on the control of inflammation. Therefore, a new stem cell-based therapy known as cell aggregates/cell sheets technology has emerged...
March 28, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29594968/profiles-of-patients-who-use-marijuana-for-inflammatory-bowel-disease
#6
Ann Marie Kerlin, Millie Long, Michael Kappelman, Christopher Martin, Robert S Sandler
BACKGROUND: Marijuana is legal in a number of states for indications that include inflammatory bowel diseases (IBD), and patients are interested in its potential benefits. AIMS: We aimed to describe the legal use of marijuana in individuals with IBD in the USA who participate within the CCFA Partners internet-based cohort. METHODS: A total of 2357 participants who lived in states where prescription or recreational marijuana was legal, were offered the opportunity to complete a survey on marijuana use and IBD symptoms including perceived benefits of therapy...
March 29, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29584538/reducing-fear-of-reinjury-and-pain-perception-among-athletes-with-first-time-anterior-cruciate-ligament-reconstructions-by-implementing-imagery-training
#7
Rosa M Rodriguez, Ashley Marroquin, Nicole Cosby
Injuries are an inevitable part of competitive sports as well a recreational activities. Research shows that one of the most common musculoskeletal complaints that accounts for approximately 48 out of 1,000 patients are knee related injuries.1 As noted, approximately nine percent of these knee complaints are related to anterior cruciate ligament (ACL) injuries.2 ,3 The ACL is commonly injured in contact sports via a non-contact action such as planting, cutting, landing on a hyperextended knee, or pivoting and sudden deceleration...
March 27, 2018: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/29578310/insights-into-islet-differentiation-and-maturation-through-proteomic-characterization-of-a-human-ipsc-derived-pancreatic-endocrine-model
#8
Corinne Haller, Prasad Chaskar, Julie Piccand, Ornella Cominetti, Charlotte Macron, Loïc Dayon, Marine R-C Kraus
PURPOSE: Great progresses have been made for generating in vitro pluripotent stem cell pancreatic β-like cells. However, the maturation stage of the cells still requires in vivo maturation to recreate the environmental niche. A deeper understanding of the factors promoting maturation of the cells is of great interest for clinical applications. EXPERIMENTAL DESIGN: We performed label-free mass spectrometry-based proteomic analysis of samples from a longitudinal study of differentiation of human-induced pluripotent stem cells towards glucose-responsive insulin producing cells...
March 26, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29577614/fully-automated-searching-for-the-optimal-vmat-jaw-settings-based-on-eclipse-scripting-application-programming-interface-esapi-and-rapidplan-knowledge-based-planning
#9
Yuliang Huang, Haizhen Yue, Meijiao Wang, Sha Li, Jian Zhang, Zhuolun Liu, Yibao Zhang
PURPOSE: Eclipse treatment planning system has not been able to optimize the jaw positions for Volumetric Modulated Arc Therapy (VMAT). The arbitrary and planner-dependent jaw placements define the maximum field size within which multi-leaf-collimator (MLC) sequences can be optimized to modulate the beam. Considering the mechanical constraints of MLC transitional speed and range, suboptimal X jaw settings may impede the optimization or undermine the deliverability. This work searches optimal VMAT jaw settings automatically based on Eclipse Scripting Application Programming Interface (ESAPI) and RapidPlan knowledge-based planning...
March 25, 2018: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/29576423/iatrogenic-pathology-of-the-urinary-bladder
#10
REVIEW
Antonio Lopez-Beltran, Rodolfo Montironi, Maria R Raspollini, Liang Cheng, George J Netto
Intravesical immunotherapy, chemotherapy, and neoadjuvant systemic chemotherapy are among the most frequent therapeutic procedures to treat malignancies of the urinary bladder. These treatment modalities produce reactive morphologic changes in the urothelium that can mimic urothelial carcinoma in situ, urothelial dysplasia or true invasive urothelial neoplasia. Mitomycin C used after transurethral resection of bladder tumor to reduce recurrences, BCG intravesical immunotherapy to treat high risk non-muscle invasive bladder cancer and urothelial carcinoma in situ, and platinum-based systemic chemotherapy to improve post-cystectomy disease-specific survival some of the causes of therapy related atypia in urinary bladder...
March 14, 2018: Seminars in Diagnostic Pathology
https://www.readbyqxmd.com/read/29565619/cultural-processes-in-psychotherapy-for-perinatal-loss-breaking-the-cultural-taboo-against-perinatal-grief
#11
Rayna D Markin, Sigal Zilcha-Mano
This paper argues that there is a cultural taboo against the public recognition and expression of perinatal grief that hinders parents' ability to mourn and their psychological adjustment following a loss. It is proposed that this cultural taboo is recreated within the therapy relationship, as feelings of grief over a perinatal loss are minimized or avoided by the therapist and parent or patient. Importantly, it is suggested that if these cultural dynamics are recognized within the therapy relationship, then psychotherapy has the immense opportunity to break the taboo by validating the parent's loss as real and helping the parent to mourn within an empathic and affect-regulating relationship...
March 2018: Psychotherapy
https://www.readbyqxmd.com/read/29559070/anova-health-institute
#12
Johannes M Hugo, Kevin B Rebe, Evan Tsouroulis, Anthony Manion, Glenn de Swart, Helen Struthers, James A McIntyre
Chemsex is the colloquial term used for a specific pattern of drug use that is increasingly common among men who have sex with men (MSM) globally. The recreational substances employed are used specifically in a sexualized context. The reasons for chemsex among MSM are complex. The Anova Health Institute (Anova) provided harm-reduction services in Cape Town, South Africa in 2013 and 2014. This project, known as Tikking the Boxes had two objectives: first to provide direct harm-reduction services to drug-using MSM in Cape Town, South Africa, and second, to reduce HIV and hepatitis B and C transmission among this population...
March 21, 2018: Sexual Health
https://www.readbyqxmd.com/read/29536940/simulation-and-the-diagnostic-process-a-pilot-study-of-trauma-and-rapid-response-teams
#13
Lindsay L Juriga, David J Murray, John R Boulet, James J Fehr
BACKGROUND: Simulation is frequently used to recreate many of the crises encountered in patient care settings. Teams learn to manage these crises in an environment that maximizes their learning experiences and eliminates the potential for patient harm. By designing simulation scenarios that include conditions associated with diagnostic errors, teams can experience how their decisions can lead to errors. The purpose of this study was to assess how trauma teams (TrT) and pediatric rapid response teams (RRT) managed scenarios that included a diagnostic error...
November 27, 2017: Diagnosis
https://www.readbyqxmd.com/read/29535188/dystrophin-s-central-domain-forms-a-complex-filament-that-becomes-disorganized-by-in-frame-deletions
#14
Olivier Delalande, Anne-Elisabeth Molza, Raphael Dos Santos-Morais, Angélique Chéron, Émeline Pollet, Céline Raguenes-Nicol, Christophe Tascon, Emmanuel Giudice, Marine Guilbaud, Aurélie Nicolas, Arnaud Bondon, France Leturcq, Nicolas Férey, Marc Baaden, Javier Perez, Pierre Roblin, France Piétri-Rouxel, Jean-François Hubert, Mirjam Czjzek, Elisabeth Le Rumeur
Dystrophin, encoded by the DMD gene, is critical for maintaining plasma membrane integrity during muscle contraction events. Mutations in the DMD gene disrupting the reading frame prevent dystrophin production and result in the high severe Duchenne muscular dystrophy (DMD); in-frame internal deletions allow production of partly functional internally deleted dystrophin and result in the less severe Becker muscular dystrophy (BMD). Many known BMD deletions occur in dystrophin's central domain, generally considered to be a monotonous rod-shaped domain based on the knowledge of spectrin-family proteins...
March 13, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29512125/evaluation-of-marijuana-compounds-on-neuroimmune-endpoints-in-experimental-autoimmune-encephalomyelitis
#15
Barbara L F Kaplan
Cannabinoid compounds refer to a group of more than 60 plant-derived compounds in Cannabis sativa, more commonly known as marijuana. Exposure to marijuana and cannabinoid compounds has been increasing due to increased societal acceptance for both recreational and possible medical use. Cannabinoid compounds suppress immune function, and while this could compromise one's ability to fight infections, immune suppression is the desired effect for therapies for autoimmune diseases. It is critical, therefore, to understand the effects and mechanisms by which cannabinoid compounds alter immune function, especially immune responses induced in autoimmune disease...
February 21, 2018: Current Protocols in Toxicology
https://www.readbyqxmd.com/read/29486610/a-multicenter-double-blind-phase-iii-clinical-trial-to-evaluate-the-efficacy-and-safety-of-a-cell-and-gene-therapy-in-knee-osteoarthritis-patients
#16
Myung-Ku Kim, Chul-Won Ha, Yong In, Sung-Do Cho, Eui-Sung Choi, Jeong-Ku Ha, Ju-Hong Lee, Jae-Doo Yoo, Seong-Il Bin, Choong-Hyeok Choi, Hee-Soo Kyung, Myung-Chul Lee
OBJECTIVE: To test the clinical efficacy of TissueGene-C (TG-C), a cell and gene therapeutic for osteoarthritis consisting of non-transformed and transduced chondrocytes (3:1), retrovirally transduced to overexpress TGF-β1. DESIGN: We randomly assigned 163 with knee osteoarthritis to receive intra-articular TG-C or placebo in Kellgren-Lawrence grade 3 patients for clinical trial. Primary efficacy measures included criteria for subjective assessment by International Knee Documentation Committee (IKDC) and pain severity by Visual Analog Scale (VAS) for 52 weeks...
February 27, 2018: Human Gene Therapy. Clinical Development
https://www.readbyqxmd.com/read/29481447/photobiomodulation-therapy-on-physiological-and-performance-parameters-during-running-tests-dose-response-effects
#17
Rodolfo André Dellagrana, Mateus Rossato, Raphael Luiz Sakugawa, Bruno Mafredini Baroni, Fernando Diefenthaeler
This study was aimed at verifying effects of photobiomodulation therapy (PBMT) with different energy doses (15, 30, and 60 J per site) on physiological and performance parameters during running tests. Fifteen male recreational runners participated in a crossover, randomised, double-blind, and placebo-controlled trial. They performed testing protocol in 5 sessions with different treatments: control, placebo, and PBMT with 15, 30 or 60 J per site (14 sites in each lower limb). Physiological and performance variables were assessed during submaximal (at 8 km·h-1 and 9 km·h-1) and maximal running tests...
February 22, 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29477032/3d-3-culture-a-tool-to-unveil-macrophage-plasticity-in-the-tumour-microenvironment
#18
Sofia P Rebelo, Catarina Pinto, Tatiana R Martins, Nathalie Harrer, Marta F Estrada, Pablo Loza-Alvarez, José Cabeçadas, Paula M Alves, Emilio J Gualda, Wolfgang Sommergruber, Catarina Brito
The tumour microenvironment (TME) shapes disease progression and influences therapeutic response. Most aggressive solid tumours have high levels of myeloid cell infiltration, namely tumour associated macrophages (TAM). Recapitulation of the interaction between the different cellular players of the TME, along with the extracellular matrix (ECM), is critical for understanding the mechanisms underlying disease progression. This particularly holds true for prediction of therapeutic response(s) to standard therapies and interrogation of efficacy of TME-targeting agents...
May 2018: Biomaterials
https://www.readbyqxmd.com/read/29463075/adverse-effects-of-doping-with-anabolic-androgenic-steroids-aas-in-competitive-athletics-recreational-sports-and-bodybuilding
#19
Elena Vorona, Eberhard Nieschlag
Despite the fact that sports organizations and legislators have introduced various mechanisms to discourage athletes from using performance and appearance enhancing substances a high percentage of athletes admits to their unabated application. In competitive athletics, bodybuilding and in recreational sports anabolic androgenic steroids (AAS) continue to be the substances most abused. This review summarizes the side effects of AAS abuse on organs and system functions in both sexes. High doses of AAS cause a significant increase of erythrocytes und haemoglobin concentration, which may lead to thromboembolism, intracardiac thrombosis and stroke...
February 19, 2018: Minerva Endocrinologica
https://www.readbyqxmd.com/read/29456518/environmental-physiology-and-diving-medicine
#20
REVIEW
Gerardo Bosco, Alex Rizzato, Richard E Moon, Enrico M Camporesi
Man's experience and exploration of the underwater environment has been recorded from ancient times and today encompasses large sections of the population for sport enjoyment, recreational and commercial purpose, as well as military strategic goals. Knowledge, respect and maintenance of the underwater world is an essential development for our future and the knowledge acquired over the last few dozen years will change rapidly in the near future with plans to establish secure habitats with specific long-term goals of exploration, maintenance and survival...
2018: Frontiers in Psychology
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