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culture and sensitivity tests

Amit Kumar Singh, Loveleena Agarwal, Akash Kumar, Chandrim Sengupta, Ravinder Pal Singh
Introduction: The study aimed to determine the prevalence of nasal colonization of methicillin-resistant Staphylococcus aureus (MRSA), the minimum inhibitory concentration (MIC) of oxacillin and vancomycin, inducible clindamycin resistance, and antimicrobial resistance pattern of S. aureus among children of Barabanki district, Uttar Pradesh, India. Materials and Methods: School-going children of age group of 5-15 years were identified and selected according to the inclusion and exclusion criteria...
January 2018: Journal of Family Medicine and Primary Care
Eduard O Roos, Francisco Olea-Popelka, Peter Buss, Lin-Mari de Klerk-Lorist, David Cooper, Robin M Warren, Paul D van Helden, Sven D C Parsons, Michele A Miller
Bovine tuberculosis (bTB) is endemic in several areas of South Africa and has been reported in multiple species, including common warthogs (Phacochoerus africanus). Limited diagnostic tests and disease control programs exist for African wildlife. Thus, there is a need to develop techniques for bTB detection in species such as warthogs to assess their role in disease maintenance and spread in multi-host ecosystems. In this study, we obtained blood samples from warthogs in bTB endemic areas to investigate biomarkers for detection of Mycobacterium bovis infection...
July 2018: Veterinary Immunology and Immunopathology
Tatiana Metcalf, Jaime Soria, Silvia M Montano, Eduardo Ticona, Carlton A Evans, Luz Huaroto, Matthew Kasper, Eric S Ramos, Nicanor Mori, Podjanee Jittamala, Kesinee Chotivanich, Irwin F Chavez, Pratap Singhasivanon, Sasithon Pukrittayakamee, Joseph R Zunt
BACKGROUND: Meningitis caused by Mycobacterium tuberculosis is a major cause of morbidity and mortality worldwide. We evaluated the performance of cerebrospinal fluid (CSF) testing with the GeneXpert MTB/RIF assay versus traditional approaches for diagnosing tuberculosis meningitis (TBM). METHODS: Patients were adults (n = 37) presenting with suspected TBM to the Hospital Nacional Dos de Mayo, Lima, Peru, during 12 months until 1st January 2015. Each participant had a single CSF specimen that was divided into aliquots that were concurrently tested for M...
2018: PloS One
Noordiana Nordin, Nor Azah Yusof, Son Radu, Roozbeh Hushiarian
Vibrio parahaemolyticus (V. parahaemolyticus) is a common foodborne pathogen that contributes to a large proportion of public health problems globally, significantly affecting the rate of human mortality and morbidity. Conventional methods for the detection of V. parahaemolyticus such as culture-based methods, immunological assays, and molecular-based methods require complicated sample handling and are time-consuming, tedious, and costly. Recently, biosensors have proven to be a promising and comprehensive detection method with the advantages of fast detection, cost-effectiveness, and practicality...
June 3, 2018: Journal of Visualized Experiments: JoVE
J Su, A Delaney, R Matteo, B Bartlett, K Kultima, T Hökfelt, C I Svensson
Aims The lysophosphatidic acid (LPA) is an important mediator involved in neuropathic and bone cancer pain models. We are investigating if LPA is involved in arthritis-induced pain in the collagen antibody-induced arthritis (CAIA) mice model. Methods Arthritis was induced in male CBA mice by injection of 1.5 mg collagen type II antibody cocktail. Mechanical and thermal sensitivity and the degree of arthritis were assessed with von Frey filaments, Hargreaves box (heat), acetone test (cold) and visual scoring, respectively...
December 29, 2017: Scandinavian Journal of Pain
Alessandra Oliva, Maria Teresa Mascellino, Bich Lien Nguyen, Massimiliano De Angelis, Alessia Cipolla, Annalisa Di Berardino, Antonio Ciccaglioni, Claudio Maria Mastroianni, Vincenzo Vullo
Introduction: Sonication showed more sensitivity than traditional culture in the diagnosis of device infections. Aims of the study were to assess the role of sonication in the microbiological diagnosis and management of cardiac device infections (CDIs), to evaluate the sensitivity of sonication in patients on antimicrobial therapy at the time of device removal, and to analyze biofilm formation of the isolated strains. Materials and Methods: A total of 90 devices (31 generators and 59 electrodes) collected from 31 patients with infection underwent sonication before culture...
April 2018: Journal of Global Infectious Diseases
Magdalena Kowalewicz-Kulbat, Piotr Szpakowski, Camille Locht, Franck Biet, Paulina Kaplonek, Krzysztof T Krawczyk, Joël Pestel, Wieslawa Rudnicka
Bacillus Calmette-Guérin (BCG) is the only vaccine available against tuberculosis and the tuberculin skin test (TST) is the most widely used method to detect BCG take. However, subjects may remain TST-negative, even after several BCG administrations. To investigate some of the potential reasons underlying this inability of developing tuberculin sensitivity in response to BCG we compared the effect of different mycobacterial stimuli in the groups differently responding to tuberculin. TST was performed on 71 healthy adults aged 25-30 years, who had received BCG in their childhood, and considered TST-positive at ≥10 mm...
June 13, 2018: Vaccine
Roseclea Chagas Dos Santos, Flaviane Alves de Pinho, Gabriela Porfírio Passos, Daniela Farias Larangeira, Stella Maria Barrouin-Melo
The most commonly used culture medium for the in vitro isolation of Leishmania spp. from canine biological samples is biphasic Novy-MacNeal-Nicolle (NNN) medium, whose solid phase is prepared using rabbit blood. Leishmania infantum parasites from natural infections are highly sensitive and demanding for growth in axenic conditions when firstly obtained from the dog's body. The objective of this study was to evaluate whether NNN medium (NNN-test) prepared with chicken blood (NNN-C), ox blood (NNN-O), horse blood (NNN-H) or sheep blood (NNN-S) was viable for the isolation of parasites from naturally infected dogs, in an endemic area for visceral leishmaniasis caused by L...
June 15, 2018: Veterinary Parasitology
Cristina Estirado, Adrian Ceccato, Monica Guerrero, Arturo Huerta, Catia Cilloniz, Olivia Vilaró, Albert Gabarrús, Joaquim Gea, Ernesto Crisafulli, Nestor Soler, Antoni Torres
BACKGROUND: Antimicrobial treatment for acute exacerbations of chronic obstructive pulmonary disease (AECOPD) remains controversial. In some cases AECOPD are caused by microorganisms that are resistant to treatments recommended by guidelines. Our aims were: 1) identify the risk factors associated with infection by microorganisms resistant to conventional treatment (MRCT), 2) Compare the clinical characteristics and outcomes of patients with AECOPD resulting from MRCT against those with AECOPD from other causes...
June 15, 2018: Respiratory Research
Z Kozovska, A Patsalias, V Bajzik, E Durinikova, L Demkova, S Jargasova, B Smolkova, J Plava, L Kucerova, M Matuskova
BACKGROUND: Recent evidence in cancer research, developed the notion that malignant tumors consist of different subpopulations of cells, one of them, known as cancer stem cells, being attributed many important properties such as enhanced tumorigenicity, proliferation potential and profound multidrug resistance to chemotherapy. Several key stem cells markers were identified in colon cancer. In our study we focused on the aldehyde dehydrogenase type 1 (ALDH1) expression in colon cancer-derived cell lines HT-29/eGFP, HCT-116/eGFP and LS-180/eGFP, and its role in the chemoresistance and tumorigenic potential...
June 15, 2018: BMC Cancer
Ann F Garland, Negussie Deyessa, Menelik Desta, Atalay Alem, Tigist Zerihun, Kristopher G Hall, Nicole Goren, Irving Fish
Introduction : Depression is associated with negative social, economic, and family outcomes and the majority of individuals with depression in low and middle income countries (LMICs) are untreated. A critical first step in bridging the treatment gap is accurate, feasible, and culturally appropriate screening to identify those who need treatment. The WHO's Perceived Well-Being Index (WHO-5) well-being instrument can potentially meet the screening needs of LMICs in primary care and community-based settings. This study tested the feasibility and validity of this tool to identify depression among adult parents of young children in Addis Ababa, Ethiopia...
June 2018: Families, Systems & Health: the Journal of Collaborative Family Healthcare
Vladimir Mouraviev, Michael McDonald
INTRODUCTION: The changing face of current infection phenotypes from planktonic to biofilm type has been developed implicating bacterial biofilms in recurrent infection. To date, no specific medical treatment exists to specifically target biofilms in the human host. Similarly, the identification of a biofilm has relied upon the analysis of tissue samples with electron microscopy or DNA identification with polymerase chain reaction (PCR) and sequencing. Standard culture and sensitivity test is not able to detect a presence of biofilms...
June 2018: Canadian Journal of Urology
Tzu-En Lin, Stefania Rapino, Hubert H Girault, Andreas Lesch
The technological and experimental progress in electrochemical imaging of biological specimens is discussed with a view on potential applications for skin cancer diagnostics, reproductive medicine and microbial testing. The electrochemical analysis of single cell activity inside cell cultures, 3D cellular aggregates and microtissues is based on the selective detection of electroactive species involved in biological functions. Electrochemical imaging strategies, based on nano/micrometric probes scanning over the sample and sensor array chips, respectively, can be made sensitive and selective without being affected by optical interference as many other microscopy techniques...
May 28, 2018: Chemical Science
Elsa Prudent, Hubert Lepidi, Emmanouil Angelakis, Didier Raoult
Purpose. Endocarditis and vascular infections are common manifestations of persistent localized infection due to Coxiella burnetii and recently, fluorescence in situ hybridization (FISH) was proposed as an alternative tool for their diagnosis. In this study, we evaluated the efficiency of FISH in a series of valve and vascular samples infected by C. burnetii. Methods. We tested 23 C. burnetii -positive valves and thrombus samples obtained from patients with Q fever endocarditis. Seven aneurysms and thrombus specimens were retrieved from patients with Q fever vascular infection...
June 13, 2018: Journal of Clinical Microbiology
Ricarda Friedrich, Elfriede Rappold, Christian Bogdan, Jürgen Held
Background (1→3)-β-D-glucan (BDG) is a biomarker for invasive fungal disease. Until now, all BDG-data in the western hemisphere were obtained using the Fungitell® assay (FA). How it compares to the Wako β-Glucan Test (GT), that was recently launched in Europe, is largely unknown. Methods We conducted a case-control study to compare both assays in serum samples of 120 candidemia and 63 Pneumocystis jirovecii pneumonia (PCP) patients. 200 patients with bacteremia or negative blood cultures served as candidemia control group...
June 13, 2018: Journal of Clinical Microbiology
Ronaldo Rodrigues da Costa, Suzane Fernandes da Silva, Romário Costa Fochat, Raquel Leite Macedo, Thamiris Vilela Pereira, Marcio Roberto Silva, Carmen Perches Gomide Pinto, Isabel Cristina Gonçalves Leite
OBJECTIVE: To compare the performance of the Ogawa-Kudoh method with the modified Petroff technique in diagnosis of pulmonary tuberculosis. METHODS: A total of 205 sputum samples from 166 patients with clinical suspicion or under pulmonary tuberculosis follow-up, seen at a public tertiary care hospital, from July 2014 to July 2016 were used. All samples were simultaneously processed using the Ogawa-Kudoh and modified Petroff decontamination methods, according to the recommendations of the Ministry of Health...
June 11, 2018: Einstein
Laurent Dortet, Didier Tandé, Dominique de Briel, Sandrine Bernabeu, Camille Lasserre, Guillaume Gregorowicz, Agnès B Jousset, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated three MALDI-TOF-based techniques to detect carbapenemase-producing Enterobacteriaceae (CPE) from cultured colonies. Methods: The performance of three MALDI-TOF-based techniques, including the commercialized MBT STAR®-Carba IVD Kit (Bruker Daltonics) and two in-house protocols performed on the Microflex LT Biotyper (Bruker Daltonics) and the VITEK® MS Plus (bioMérieux), were compared with those of the RAPIDEC® CARBA NP (bioMérieux)...
June 11, 2018: Journal of Antimicrobial Chemotherapy
Hiba Zaaroura, Yuval Geffen, Reuven Bergman, Emily Avitan-Hersh
Staphylococcus lugdunensis is an emerging pathogen in skin and soft tissue infections that was previously considered a commensal. The aim of this study was to elucidate the characteristics of skin infections by S. lugdunensis and its appropriate management, in a tertiary referral medical center. The clinical files, bacterial cultures and histopathology reports of all S. lugdunensis isolates from skin infections over a period of 8 years (September 2009-September 2017) were reviewed. S. lugdunensis was isolated from 29 patients with skin infections, aged 7-89 years (mean 33...
June 13, 2018: Journal of Dermatology
Elisabeth Wehrle-Wieland, Kristina Affolter, Daniel Goldenberger, Sarah Tschudin Sutter, Joerg Halter, Jakob Passweg, Michael Tamm, Nina Khanna, Daiana Stolz
BACKGROUND: Accurate diagnosis of invasive mold diseases (IMD) remains challenging. Here, the performance of panfungal PCR, Aspergillus and Mucorales PCR in bronchoalveolar lavage (BAL) was evaluated. METHODS: We conducted a single-center study including 167 hematologic patients at risk for IMD with BAL performed 2011-2014. Diagnostic performance of single tests (Aspergillus-, Mucorales-and panfungal PCR, galactomannan (GM)≥0.5 and ≥1, culture/cytology) or in combination was calculated for predicting IMD comparing proven/probable or proven/probable/possible IMD versus no IMD, respectively...
June 13, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
Baoning Wang, Li Wan, Yongjun Zhou, Mingjiang Bie, Zhenmei An, Hengjian Huang, Chengzhi Tang
To carry out phylogenetic analysis for drug-resistance genes from clinical isolates of Helicobacter pylori (Hp) among patients with gastric diseases from Tibet, China. METHODS: Hp strains were isolated and cultured from saliva and gastric mucosal tissues derived from patients with gastric diseases. Nine strains (including 5 isolated from oral tissues, 1 isolated from gastric tissues, and 3 representative strains of SS international standard strains used for animal models) were tested for common antibiotic resistance...
June 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
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