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https://www.readbyqxmd.com/read/28535437/comparative-evaluation-of-three-fda-approved-hiv-ag-ab-combination-tests-using-a-genetically-diverse-hiv-panel-and-diagnostic-specimens
#1
Xiaoxing Qiu, Lori Sokoll, Paul Yip, Debra J Elliott, Renu Dua, Phaedre Mohr, Xiao Yan Wang, Megan Spencer, Priscilla Swanson, George J Dawson, John Hackett
BACKGROUND: HIV Ag/Ab combination assays are recommended by CDC for routine screening and several HIV Ag/Ab combination tests are now FDA-approved. Maintaining high specificity and consistent sensitivity across diverse HIV strains is critical for these assays to accurately detect HIV infection and expedite delivery of patient results. OBJECTIVES: To evaluate performance of three FDA-approved HIV tests: ARCHITECT HIV Combo (Abbott), ADVIA Centaur HIV Combo (Siemens) and BioPlex HIV Ag-Ab (Bio-Rad)...
May 13, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28533857/antibiogram-profile-of-uropathogens-isolated-at-bahir-dar-regional-health-research-laboratory-centre-northwest-ethiopia
#2
Awoke Derbie, Derese Hailu, Daniel Mekonnen, Bayeh Abera, Gashaw Yitayew
INTRODUCTION: Antimicrobial resistance among bacteria that cause urinary tract infection (UTI) has been increasing since the introduction of chemotherapy. This study was aimed to assess the types of isolates from patients with UTI and to determine their current antimicrobial susceptibility profile. METHODS: A record based retrospective analysis of bacterial uropathogens processed in the period of January 2012 to December 2014 at Bahir Dar Health Research Laboratory Center (BRHRLC) was determined...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28533127/primary-human-polarized-small-intestinal-epithelial-barriers-respond-differently-to-a-hazardous-and-an-innocuous-protein
#3
A D Eaton, C Zimmermann, B Delaney, B P Hurley
An experimental platform employing human derived intestinal epithelial cell (IEC) line monolayers grown on permeable Transwell(®) filters was previously investigated to differentiate between hazardous and innocuous proteins. This approach was effective at distinguishing these types of proteins and perturbation of monolayer integrity, particularly transepithelial electrical resistance (TEER), was the most sensitive indicator. In the current report, in vitro indicators of monolayer integrity, cytotoxicity, and inflammation were evaluated using primary (non-transformed) human polarized small intestinal epithelial barriers cultured on Transwell(®) filters to compare effects of a hazardous protein (Clostridium difficile Toxin A [ToxA]) and an innocuous protein (bovine serum albumin [BSA])...
May 19, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28532445/cross-cultural-adaptation-and-validation-of-the-spanish-version-of-the-oxford-hip-score-in-patients-with-hip-osteoarthritis
#4
Jesús Martín-Fernández, Pedro Gray-Laymón, Antonio Molina-Siguero, Javier Martínez-Martín, Roberto García-Maroto, Isidoro García-Sánchez, Lidia García-Pérez, Vanesa Ramos-García, Olga Castro-Casas, Amaia Bilbao
BACKGROUND: Osteoarthritis (OA) of the hip is a disease that entails a major burden for patients and the society as a whole. One way of measuring this burden for the patient is through impact on Health-related Quality of Life (HRQL). The Oxford Hip Score (OHS) is a well-known tool to measure HRQL in patients with OA of the hip. This study aims to assess the psychometric properties of the Spanish-adapted version of the OHS, including its reliability, validity, and sensitivity to change...
May 22, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28532188/differential-amplification-of-marek-s-disease-cvi988-vaccine-and-of-wild-type-isolates-from-organs-of-commercial-chickens-using-single-or-duplexed-probes-in-real-time-pcr
#5
Irit Davidson, Amira Natour-Altoury, Israel Raibstein, Yaad Dahan
The DIVA test, based on the MDV pp38 gene was described by Baigent et al. (2016), using similar primers and alternate probes for virulent MDV-1 and the vaccine CVI988 virus. We now explored the assay applicability for commercial vaccines and for DNAs of commercial chickens, as former study employed tissue cultured prototype MDV strains and chicken tissue from experimental infection trials. DNA of chicken visceral organs and feathers of either vaccinated, or naturally-infected with virulent MDV-1 wild-type viruses was used for DIVA-PCR real-time PCR...
May 22, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28530540/novel-n-mustard-benzimidazoles-benzothiazoles-hybrids-synthesis-and-anticancer-evaluation
#6
Dilip P Detroja, Tai-Lin Chen, Yi-Wen Lin, Tsai-Yi Yen, Ming-Hsi Wu, Tung-Hu Tsai, Krunal Maheriya, Rajesh Kakadiya, Te-Chang Lee, Anamik Shah, Tsann-Long Su
BACKGROUND: Bendamustine, an N-mustard-benzoimidazole hybrid conjugate, was recently approved for the treatment of chronic lymphocytic leukemia. However, the short half-life of bendamustine may limit its clinical applications. OBJECTIVE: The purpose of this study is to design and synthesize compounds with a more favorable pharmacokinetic profile. METHODS: We synthesized a series of hybrid molecules comprising a phenyl N-mustard moiety and benzothiazole or benzimidazole scaffold linked via an ureido linker and evaluated their antitumor activity and plasma stability...
May 22, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/28530022/development-of-a-reverse-transcription-quantitative-polymerase-chain-reaction-based-assay-for-broad-coverage-detection-of-african-and-asian-zika-virus-lineages
#7
Yang Yang, Gary Wong, Baoguo Ye, Shihua Li, Shanqin Li, Haixia Zheng, Qiang Wang, Mifang Liang, George F Gao, Lei Liu, Yingxia Liu, Yuhai Bi
The Zika virus (ZIKV) is an arbovirus that has spread rapidly worldwide within recent times. There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome (GBS) and microcephaly in humans. The ZIKV is genetically diverse and can be separated into Asian and African lineages. A rapid, sensitive, and specific assay is needed for the detection of ZIKV across various pandemic regions. So far, the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains...
May 19, 2017: Virologica Sinica
https://www.readbyqxmd.com/read/28529673/on-chip-two-photon-metabolic-imaging-for-drug-toxicity-testing
#8
Fang Yu, Shuangmu Zhuo, Yinghua Qu, Deepak Choudhury, Zhiping Wang, Ciprian Iliescu, Hanry Yu
We have developed a microfluidic system suitable to be incorporated with a metabolic imaging method to monitor the drug response of cells cultured on a chip. The cells were perfusion-cultured to mimic the blood flow in vivo. Label-free optical measurements and imaging of nicotinamide adenine dinucleotide and flavin adenine dinucleotide fluorescence intensity and morphological changes were evaluated non-invasively. Drug responses calculated using redox ratio imaging were compared with the drug toxicity testing results obtained with a traditional well-plate system...
May 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28529026/the-use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-bacterial-identification-in-patients-with-smear-positive-bacterial-meningitis
#9
Boaz Bishop, Yuval Geffen, Adi Plaut, Ola Kassis, Roni Bitterman, Mical Paul, Ami Neuberger
OBJECTIVES: To assess the potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in rapid identification of bacteria from smear-positive cerebrospinal fluid (CSF) in a cohort of patients with meningitis. METHODS: Single-center observational study, including adults and children with community-acquired or postneurosurgical bacterial meningitis. Meningitis was defined using established criteria. CSF samples that had a positive CSF Gram stain were directly examined by MALDI-TOF-MS...
May 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28527697/antibiotics-induce-mitonuclear-protein-imbalance-but-fail-to-inhibit-respiration-and-nutrient-activation-in-pancreatic-%C3%AE-cells
#10
Jaime Santo-Domingo, Isabelle Chareyron, Charlotte Broenimann, Steve Lassueur, Andreas Wiederkehr
Chloramphenicol and several other antibiotics targeting bacterial ribosomes inhibit mitochondrial protein translation. Inhibition of mitochondrial protein synthesis leads to mitonuclear protein imbalance and reduced respiratory rates as confirmed here in HeLa and PC12 cells. Unexpectedly, respiration in INS-1E insulinoma cells and primary human islets was unaltered in the presence of chloramphenicol. Resting respiratory rates and glucose stimulated acceleration of respiration were also not lowered when a range of antibiotics including, thiamphenicol, streptomycin, gentamycin and doxycycline known to interfere with bacterial protein synthesis were tested...
May 17, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28527280/-pperformance-of-led-fluorescence-microscopy-for-the-detection-of-acid-fast-bacilli-from-respiratory-samples-in-peripheral-laboratories-in-argentina
#11
María Imaz, Sonia Allassia, Mónica Aranibar, Alba Gunia, Susana Poggi, Ana Togneri, Lidia Wolff, Group Of Implementation Of Fluorescence
INTRODUCTION: Light-emitting diode fluorescence microscopy (LED-FM) has been endorsed by the World Health Organization (WHO) for tuberculosis diagnosis, but its accuracy in HIV-infected patients remains controversial, and only some few studies have explored procedural factors that may affect its performance. OBJECTIVE: To evaluate the performance of LED-FM for tuberculosis diagnosis in patients with and without HIV infection using a newer, less expensive LED lamp...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527125/diversity-and-probiotic-potential-of-lactic-acid-bacteria-isolated-from-horreh-a-traditional-iranian-fermented-food
#12
Alireza Vasiee, Behrooz Alizadeh Behbahani, Farideh Tabatabaei Yazdi, Seyed Ali Mortazavi, Hamid Noorbakhsh
The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) strains isolated from Horreh. Some probiotic properties, e.g., resistance to acid, bile tolerance, antibacterial activity, and antibiotic susceptibility, were investigated. A total of 140 Gram-positive and catalase-negative isolates from Horreh were subjected to identification and grouping by cultural methods and the 16S rRNA sequencing. The new isolates were identified to be Lactobacillus (fermentum, plantarum, and brevis) Weissella cibaria, Enterococcus (faecium and faecalis), Leuconostoc (citreum and mesenteroides subsp...
May 19, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28525324/in-vitro-effect-of-leptin-on-anterior-pituitary-cells-lh-secretory-activity-during-early-pregnancy-in-pig
#13
G Siawrys, A Gajewska
Leptin modulates reproductive activity but its potential influence on LH secretion from anterior pituitary (AP) cells during implantation period in pigs (days 14-16 of pregnancy) remained unexplored. This study focused on determination whether leptin affects basal and GnRH-induced LH secretion and intracellular accumulation and whether leptin receptor (OB-Rb) mRNA is expressed in the AP gland during implantation in pigs. Four individual AP glands were developed into separate primary cultures. 2×105 cells/ml were preincubated (72 h) and next, for 3...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28523017/antibiotic-susceptibility-in-commonly-isolated-pathogens-from-urinary-tract-infection-in-a-cohort-of-subjects-from-low-socioeconomic-strata
#14
Saera Suhail Kidwai, Ayesha Nageen, Samina Ghaznavi, Farhat Bashir, Jamal Ara
BACKGROUND AND OBJECTIVE: Urinary tract infection is one of the commonest infections seen in clinical practice. Lack of compliance and unjustified antibiotic prescriptions has resulted in bacterial resistance and is proving as a major challenge in the management of these infections. Our aim was to identify the sensitivity pattern of commonly used antibiotics against urinary tract infections so as to suggest an improvised line of action against bacteria causing urinary tract infections'...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28522948/evaluation-of-xpert-mtb-rif-assay-performance-in-the-diagnosis-of-abdominal-tuberculosis
#15
Suraj Kumar, Sawan Bopanna, Saurabh Kedia, Pratap Mouli, Rajan Dhingra, Rajesh Padhan, Mikashmi Kohli, Jigyasa Chaubey, Rohini Sharma, Prasenjit Das, S Dattagupta, Govind Makharia, S K Sharma, Vineet Ahuja
BACKGROUND/AIMS: The use of genetic probes for the diagnosis of pulmonary tuberculosis (TB) has been well described. However, the role of these assays in the diagnosis of intestinal tuberculosis is unclear. We therefore assessed the diagnostic utility of the Xpert Mycobacterium tuberculosis/rifampicin (MTB/RIF) assay, and estimated the prevalence of multidrug-resistant (MDR) TB in the Indian population. METHODS: Of 99 patients recruited, 37 had intestinal TB; two control groups comprised 43 with Crohn's disease (CD) and 19 with irritable bowel syndrome...
April 2017: Intestinal Research
https://www.readbyqxmd.com/read/28520594/amplivue-is-a-practical-and-timely-test-for-the-detection-of-hsv-from-keratitis-specimens
#16
Regis P Kowalski, Lisa M Karenchak, Deepinder K Dhaliwal, Alex Mammen
OBJECTIVE: The laboratory diagnostic detection of herpes simplex virus (HSV) from eye samples must be practical, timely, and definitive for appropriate therapy. Although polymerase chain reaction (PCR) and/or cell culture can be definitive, HSV results can be delayed. Enzyme Linked Virus Inducible System (ELVIS) is a test that can provide results within 24 to 48 hr. We evaluated "AmpliVue HSV 1+2 Assay" as a molecular colorimetric test that can detect HSV (1 or 2) DNA within 1 hr. METHODS: Cornea/conjunctival samples were tested retrospectively with AmpliVue against 53 true-positive and 20 true-negative specimens collected in chlamydial transport medium...
May 16, 2017: Eye & Contact Lens
https://www.readbyqxmd.com/read/28516225/a-case-of-bilateral-keratitis-caused-by-providencia-alcalifaciens-a-rarely-encountered-ocular-pathogen
#17
Won Choi, Yong Sok Ji, Kyung Chul Yoon
PURPOSE: To report a case of keratitis caused by Providencia alcalifaciens. METHODS: A 70-year-old man presented with pain, redness, and decreased vision in both of his eyes. On initial presentation, the visual acuity in both of his eyes was hand motion, and slit lamp examination showed diffuse infiltrates and total epithelial defects of the cornea, conjunctival hyperemia, and severe anterior chamber reactions with 2-mm-height hypopyon. The results of laboratory studies revealed elevated liver function tests, which indicated the presence of alcoholic liver disease...
May 17, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28515219/a-novel-stool-pcr-test-for-helicobacter-pylori-may-predict-clarithromycin-resistance-and-patient-eradication-at-a-high-rate
#18
Erin Beckman, Ilaria Saracino, Giulia Fiorini, Courtney Clark, Vladimir Slepnev, Denise Patel, Clarissa Gomez, Reddy Ponaka, Vecheslav Elagin, Dino Vaira
Objectives: Clarithromycin-based regimens are commonly used as a first line therapy in H. pylori positive patients, however treatment failures are associated with resistance to clarithromycin. The aim of this study was to evaluate the feasibility of using stool samples to detect the presence of H. pylori DNA, while concurrently detecting mutations associated with the resistance to clarithromycin.Methods: Total DNA was extracted from 294 raw stools of H pylori positive and negative patients. TaqMan real-time PCR amplification was used to detect the presence of H...
May 17, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28515032/bilateral-painless-testicular-mass-acute-uveitis-and-annular-cutaneous-lesions-an-unusual-presentation-of-sarcoidosis-and-literature-review
#19
Enver Şimşek, Tülay Şimşek, Meliha Demiral, Funda Canaz, M Fuat Açıkalın
BACKGROUND: Sarcoidosis is a systemic inflammatory disease characterized by non-caseating epitheloid granulomas; whereas it usually involves the lungs and lymph nodes, genitourinary involvement is extremely rare. For this reason, no consensus regarding the diagnostic and management options for testicular sarcoidosis has been reached. OBJECTIVE: The aim of this case report was to describe an unusual clinical presentation of sarcoidosis. An 8-year-old boy presented with a 6-month history of painless scrotal enlargement and a 2-month history of photophobia, blurred vision, and pain in both eyes...
May 17, 2017: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/28513759/-diagnosis-of-leptospirosis-in-dogs
#20
Michèle Bergmann, Julia-Rebecca Llewellyn, Katrin Hartmann
Rapid confirmation of the diagnosis leptospirosis is important in order to apply appropriate treatment; in addition, infected dogs are a zoonotic risk. Culture and isolation of living leptospires from blood, urine, or tissues is considered the reference standard of diagnosis. However, sensitivity are low, and leptospires require weeks to months to grow. Therefore, diagnosis of leptospirosis is most commonly based on antibody testing or the detection of Leptospira spp. DNA by PCR. Microscopic agglutination test (MAT) is currently still the recommended confirmatory test for canine leptospirosis, despite its numerous limitations (e...
May 17, 2017: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
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