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https://www.readbyqxmd.com/read/29055192/electrochemical-immunosensor-with-nanocellulose-au-composite-assisted-multiple-signal-amplification-for-detection-of-avian-leukosis-virus-subgroup-j
#1
Chao Liu, Jing Dong, Geoffrey I N Waterhouse, Ziqiang Cheng, Shiyun Ai
A sensitive sandwich-type electrochemical immunosensor was developed for the detection of avian leukosis virus subgroup J (ALV-J), which benefitted from multiple signal amplification involving graphene-perylene-3,4,9,10-tetracarboxylic acid nanocomposites (GR-PTCA), nanocellulose-Au NP composites (NC-Au) and the alkaline phosphatase (ALP) catalytic reaction. GR-PTCA nanocomposites on glassy carbon electrodes served as the immunosensor platform. Due to their excellent electrical conductivity and abundant polycarboxylic sites, the GR-PTCA nanocomposites allowed fast electron transfer and good immobilization of primary antibodies, thereby affording a strong immunosensor signal in the presence of ALV-J...
October 3, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29053085/rifampicin-resistance-and-multidrug-resistant-tuberculosis-detection-using-xpert-mtb-rif-in-wuhan-china-a-retrospective-study
#2
Hai Huang, Yanlin Zhang, Sheng Li, Jun Wang, Jun Chen, Zhiyun Pan, Hui Gan
BACKGROUND: The Xpert MTB/RIF test (Cepheid, Sunnyvale, CA) can simultaneously detect the Mycobacterium tuberculosis (MTB) complex DNA and rifampicin (RFP) resistance and can rapidly determine RFP resistance and predict multidrug-resistant tuberculosis (MDR-TB). In this study, we analyzed clinical examination results of a hospital specializing in TB treatment in Wuhan, Hubei, China, and examined the use of traditional culture and drug-sensitive test (DST) results as a gold standard to assess the diagnosis value of the Xpert MTB/RIF test in RFP resistance and MDR-TB...
October 20, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29052953/cross-cultural-adaptation-and-validation-of-the-comprehensive-frailty-assessment-instrument-in-chinese-community-dwelling-older-adults
#3
Xiaoxia Qiao, Cuili Wang, Xiaoyu Tian, Lijuan Dong, Na Liu, Yaru Jin, Huaxin Si
AIM: To cross-culturally adapt and validate the Comprehensive Frailty Assessment Instrument (CFAI) among Chinese community-dwelling older adults. METHODS: The Chinese CFAI was developed through forward-backward translations. An urban sample of 1235 community-dwelling older adults received face-to-face interviews to examine the validity (construct validity and criterion validity) and reliability (internal consistency and test-retest reliability). RESULTS: The Chinese CFAI achieved semantic and idiomatic equivalence, and showed acceptable reliability and an expected factor structure, except for the social support domain...
October 20, 2017: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/29052293/butyrate-promotes-visceral-hypersensitivity-in-an-ibs-like-model-via-enteric-glial-cell-derived-nerve-growth-factor
#4
X Long, M Li, L-X Li, Y-Y Sun, W-X Zhang, D-Y Zhao, Y-Q Li
BACKGROUND: Altered visceral sensation is common in irritable bowel syndrome (IBS) and nerve growth factor (NGF) participates in visceral pain development. Sodium butyrate (NaB) could induce colonic hypersensitivity via peripheral up-regulation of NGF in animals. Enteric glial cells (EGCs) appear to be an important source of NGF. Whether butyrate could induce visceral hypersensitivity via increased EGC-derived NGF is still unknown. METHODS: CRL-2690 cells were used for transcriptome analyses after butyrate treatment...
October 20, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29050130/-evaluation-of-a-high-intensity-fluorescent-fluorophage-method-for-diagnosis-of-drug-resistance-in-tuberculosis
#5
J J Luo, X Yu, Y F Ma, L P Zhao, Y Y Shang, Y Y Fu, H R Huang
Objective: To evaluate the high-intensity green fluorescent protein fluorophage Φ(2)GFP10 method for drug susceptibility testing of tuberculosis for isoniazid(INH), rifampin(RIF), and streptomycin(SM). Methods: A total of 128 clinical M. tuberculosis strains were isolated from patients with suspected drug-resistant tuberculosis visiting Beijing Chest Hospital (Beijing, China) from April to June 2014.All of the isolates were tested by the phage assay, while conventional drug susceptibility tests were performing on Lwenstein-Jensen culture medium as reference...
October 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/29050115/-effects-of-ovalbumin-exposure-during-pregnancy-of-mice-on-the-ovalbumin-re-exposure-in-adult-progeny
#6
Z Song, H Q Li
Objective: To observe the immunoreaction of offspring mice by ovalbumin (OVA) re-exposure after their mothers exposed to OVA during pregnancy. Method: A prospective controlled study was conducted to observe mice after repeated OVA exposures at 6-8 weeks.Their mothers were exposed to OVA during different stages of pregnancy.The symptoms were recorded and scored.The levels of OVA-specific IgE in serum, interferon-γ(IFN-γ) and interleukin-4(IL-4) in supernatant of spleen primary lymphocytes in vitro were measured by ELISA...
October 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/29047004/age-patterns-in-risk-taking-across-the-world
#7
Natasha Duell, Laurence Steinberg, Grace Icenogle, Jason Chein, Nandita Chaudhary, Laura Di Giunta, Kenneth A Dodge, Kostas A Fanti, Jennifer E Lansford, Paul Oburu, Concetta Pastorelli, Ann T Skinner, Emma Sorbring, Sombat Tapanya, Liliana Maria Uribe Tirado, Liane Peña Alampay, Suha M Al-Hassan, Hanan M S Takash, Dario Bacchini, Lei Chang
Epidemiological data indicate that risk behaviors are among the leading causes of adolescent morbidity and mortality worldwide. Consistent with this, laboratory-based studies of age differences in risk behavior allude to a peak in adolescence, suggesting that adolescents demonstrate a heightened propensity, or inherent inclination, to take risks. Unlike epidemiological reports, studies of risk taking propensity have been limited to Western samples, leaving questions about the extent to which heightened risk taking propensity is an inherent or culturally constructed aspect of adolescence...
October 19, 2017: Journal of Youth and Adolescence
https://www.readbyqxmd.com/read/29046671/generation-of-persister-cells-of-pseudomonas-aeruginosa-and-staphylococcus-aureus-by-chemical-treatment-and-evaluation-of-their-susceptibility-to-membrane-targeting-agents
#8
Lucia Grassi, Mariagrazia Di Luca, Giuseppantonio Maisetta, Andrea C Rinaldi, Semih Esin, Andrej Trampuz, Giovanna Batoni
Persister cells (PCs) are a subset of dormant, phenotypic variants of regular bacteria, highly tolerant to antibiotics. Generation of PCs in vivo may account for the recalcitrance of most chronic infections to antimicrobial treatment and demands for the identification of new antimicrobial agents able to target such cells. The present study explored the possibility to obtain in vitro PCs of Pseudomonas aeruginosa and Staphylococcus aureus at high efficiency through chemical treatment, and to test their susceptibility to structurally different antimicrobial peptides (AMPs) and two clinically used peptide-based antibiotics, colistin and daptomycin...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29046411/rapid-detection-and-differentiation-of-clinically-relevant-candida-species-simultaneously-from-blood-culture-using-a-novel-signal-amplification-approach
#9
Wanyuan Ao, Joshua Klonoski, Eric Berlinghoff, Jordan Jensen, Taliman Afroz, Denton Munns, Wes Lindsey, Gerald Denys, Robert Jenison
Fungal blood-stream infections are a significant problem in the U.S. with an attributable mortality rate of up to 40%. Early diagnosis to direct appropriate therapy has been shown to be critical to reduce mortality rates. Conventional phenotypic methods for fungal detection take several days, which is often too late to impact outcomes. Herein, we describe a cost-effective multiplex assay platform for rapid detection and differentiation of major clinically relevant Candida species directly from blood culture...
October 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29045415/isolation-identification-and-phenotypic-and-molecular-characterization-of-pathogenic-vibrio-vulnificus-isolated-from-litopenaeus-vannamei
#10
Tao Teng, Liguo Liang, Kai Chen, Bingwen Xi, Jun Xie, Pao Xu
The morphology and the drug sensitivity of the strain GYX2014-1 isolated from the hepatic pancreatic tissue of moribund Litopenaeus vannamei were evaluated by conventional culture characteristics, physical and chemical characteristics, and molecular biology methods. Detection of extracellulase and hemolysin activity shows that the isolated GYX2014-1 has protease, lipase, gelatinase activity, but none of amylase, or lecithinase activity. The 16S rRNA gene (GenBank accession number: KT781675) was analyzed, and a phylogenetic tree analysis showed that the isolated pathogen was most closely related to V...
2017: PloS One
https://www.readbyqxmd.com/read/29044041/viral-haemorrhagic-septicaemia-virus-vhsv-id-infections-are-detected-more-consistently-using-syndromic-vs-active-surveillance
#11
Pia Vennerström, Elina Välimäki, Tapani Lyytikäinen, Maria Hautaniemi, Gabriele Vidgren, Perttu Koski, Anna-Maija Virtala
The eradication of viral haemorrhagic septicaemia virus (VHSV Id) from Finnish brackish-water rainbow trout Oncorhynchus mykiss farms located in the restriction zone in the Province of Åland, Baltic Sea, failed several times in the 2000s. The official surveillance programme was often unable to find VHSV-positive populations, leading to the misbelief in the fish farming industry that virus eradication could be achieved. The ability of 3 other surveillance programmes to detect infected fish populations was compared with the official programme...
October 18, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/29044002/irrational-antibiotic-prescribing-practice-among-children-in-critical-care-of-tertiary-hospitals
#12
Mirza Tasawer Baig, Ali Akbar Sial, Ambreen Huma, Maryam Ahmed, Uzma Shahid, Nayel Syed
This study assessed the prescribing pattern of irrational use of antibiotic among children under age of 12 years in public and private sector hospitals in Pakistan. The prospective clinical evaluation of drug utilization pattern of antimicrobials from Patient Bedside File (PBF) of in-patients and Culture Sensitivity Test (CST) reports were evaluated to determine the antibiotic resistance. Two indicators recorded to assess antibiotic prescribing were; dose of prescribed antibiotic (low-dose, rational and high -dose) and Indication (valid or invalid)...
July 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29042709/derivation-and-basic-characterization-of-colorectal-carcinoma-primary-cell-lines
#13
Lukas Krbal, Jiri Soukup, Stanislav John, Veronika Hanusova
BACKGROUND: Colorectal carcinoma is one of the most common malignancies in western countries. Among different approaches to its research, primary cancer cell lines can play an important role. AIM: The main purposes of this study were: 1) to establish an effective and reproducible method of colorectal cancer cell isolation and cultivation from primary tumours and lymph node metastases and 2) to elucidate the biological features of the tumours favouring successful cell cultivation...
October 17, 2017: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/29042614/3d-printed-poly-%C3%AE%C2%B5-caprolactone-scaffold-integrated-with-cell-laden-chitosan-hydrogels-for-bone-tissue-engineering
#14
Liang Dong, Shao-Jie Wang, Xin-Rong Zhao, Yu-Fang Zhu, Jia-Kuo Yu
Synthetic polymeric scaffolds are commonly used in bone tissue engineering (BTE) due to their biocompatibility and adequate mechanical properties. However, their hydrophobicity and the lack of specific cell recognition sites confined their practical application. In this study, to improve the cell seeding efficiency and osteoinductivity, an injectable thermo-sensitive chitosan hydrogel (CSG) was incorporated into a 3D-printed poly(ε-caprolactone) (PCL) scaffold to form a hybrid scaffold. To demonstrate the feasibility of this hybrid system for BTE application, rabbit bone marrow mesenchymal stem cells (BMMSCs) and bone morphogenetic protein-2 (BMP-2) were encapsulated in CSG...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29040875/biomechanical-regulation-of-drug-sensitivity-in-an-engineered-model-of-human-tumor
#15
A Marturano-Kruik, A Villasante, K Yaeger, S R Ambati, A Chramiec, M T Raimondi, G Vunjak-Novakovic
Predictive testing of anticancer drugs remains a challenge. Bioengineered systems, designed to mimic key aspects of the human tumor microenvironment, are now improving our understanding of cancer biology and facilitating clinical translation. We show that mechanical signals have major effects on cancer drug sensitivity, using a bioengineered model of human bone sarcoma. Ewing sarcoma (ES) cells were studied within a three-dimensional (3D) matrix in a bioreactor providing mechanical loadings. Mimicking bone-like mechanical signals within the 3D model, we rescued the ERK1/2-RUNX2 signaling pathways leading to drug resistance...
October 10, 2017: Biomaterials
https://www.readbyqxmd.com/read/29040411/handoc-a-handy-score-to-determine-the-need-for-echocardiography-in-non-beta-hemolytic-streptococcal-bacteremia
#16
Torgny Sunnerhagen, Amanda Törnell, Maria Vikbrant, Bo Nilson, Magnus Rasmussen
Background: Non-beta-hemolytic streptococci (NBHS) are a common cause of infective endocarditis (IE). Echocardiography is used to diagnose IE, but it is not known which patients with NBHS bacteremia should undergo echocardiography. Method: Medical records of patients with NBHS bacteremia in southern Sweden from 2012-2014 were studied retrospectively. The patients were divided into two cohorts. In the first, correlations between the reported data and IE were studied...
October 10, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29038627/characterization-and-antibiotic-sensitivity-profile-of-bacteria-in-orofacial-abscesses-of-odontogenic-origin
#17
H Jagadish Chandra, B H Sripathi Rao, A P Muhammed Manzoor, A B Arun
BACKGROUND: Odontogenic infections range from peripheral abscess to superficial and deep infections leading to severe infections in head and neck region. This study was aimed to assess bacterial isolates responsible for orofacial infection of odontogenic origin and their drug susceptibility patterns so as to provide better perceptive for the management of odontogenic infections. METHODS: The study was made in a selected cohort of patients, irrespective of age and gender having moderate and severe orofacial infections of odontogenic origin admitted to Yenepoya University Hospital...
December 2017: Journal of Maxillofacial and Oral Surgery
https://www.readbyqxmd.com/read/29037535/performance-of-implant-sonication-culture-for-the-diagnosis-of-periprosthetic-shoulder-infection
#18
Matthew J Grosso, Salvatore J Frangiamore, George Yakubek, Thomas W Bauer, Joseph P Iannotti, Eric T Ricchetti
BACKGROUND: Diagnosing infection after shoulder arthroplasty can be a challenge because of the high prevalence of low-virulence organisms, such as Propionibacterium acnes. The purpose of this study was to evaluate the utility of implant sonication fluid cultures in the diagnosis of periprosthetic joint infection compared with standard culture techniques in patients undergoing revision shoulder arthroplasty. METHODS: Routine perioperative testing was performed in 53 patients who underwent revision shoulder arthroplasty...
October 13, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/29037467/cross-cultural-adaptation-and-refinement-of-an-english-version-of-a-dutch-patient-reported-questionnaire-for-hand-sensitivity-the-radboud-evaluation-of-sensitivity
#19
Tara L Packham, Joy C MacDermid, Susan Michlovitz, Edith Cup, Lucelle Van de Ven-Stevens
STUDY DESIGN: Longitudinal clinical measurement. INTRODUCTION: Sensory alterations in the hand can present as both decreased sensation or numbness, and hyperesthesia, including mechanical allodynia and cold intolerance. However, few patient-reported outcomes have been developed and validated for evaluation, particularly for increased sensitivity. The Radboud Evaluation of Sensitivity was developed in the Netherlands for patient-reported evaluation of hand sensitivity in complex regional pain syndrome...
October 13, 2017: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
https://www.readbyqxmd.com/read/29037292/accuracy-of-giant-african-pouched-rats-for-diagnosing-tuberculosis-comparison-with-culture-and-xpert-%C3%A2-mtb-rif
#20
C Mulder, G F Mgode, H Ellis, E Valverde, N Beyene, C Cox, S E Reid, A H Van't Hoog, T L Edwards
SETTING: Enhanced tuberculosis (TB) case finding using detection rats in Tanzania. OBJECTIVES: To assess the diagnostic accuracy of detection rats compared with culture and Xpert® MTB/RIF, and to compare enhanced case-finding algorithms using rats in smear-negative presumptive TB patients. DESIGN: A fully paired diagnostic accuracy study in which sputum of new adult presumptive TB patients in Tanzania was tested using smear microscopy, 11 detection rats, culture and Xpert...
November 1, 2017: International Journal of Tuberculosis and Lung Disease
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