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culture and sensitivity tests

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https://www.readbyqxmd.com/read/29458007/a-genomically-characterized-collection-of-high-grade-serous-ovarian-cancer-xenografts-for-preclinical-testing
#1
Paulina Cybulska, Jocelyn M Stewart, Azin Sayad, Carl Virtanen, Patricia A Shaw, Blaise Clarke, Natalie Stickle, Marcus Q Bernardini, Benjamin G Neel
High-grade serous ovarian cancer (HGSC) is the leading cause of morbidity and mortality from gynecologic malignancy. Overall survival remains low, due to the nearly ubiquitous emergence of platinum-resistance and the paucity of effective next-line treatments. Current cell culture-based models show limited similarity to HGSC and are therefore unreliable predictive models for pre-clinical evaluation of investigational drugs. This deficiency could help explain the low overall rate of successful drug development and the decades of largely unchanged approaches to HGSC treatment...
February 16, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29457460/stressful-surfaces-cell-metabolism-on-a-poorly-adhesive-substrate
#2
Richard L Surmaitis, Carlos J Arias, Joseph B Schlenoff
The adhesion and proliferation of cells are exquisitely sensitive to the nature of the surface to which they attach. Aside from cell counting, cell "health" on surfaces is typically established by measuring the metabolic rate with dyes that participate in the metabolic pathway or using "live/dead" assays with combinations of membrane permeable/impermeable dyes. The binary information gleaned from these tests-whether cells are attached or not, and whether they are living or dead-provides an incomplete picture of cell health...
February 19, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29457285/vaginal-isolation-of-beta-haemolytic-streptococcus-from-bitches-with-and-without-neonatal-deaths-in-the-litters
#3
A E Guerrero, M C Stornelli, S B Jurado, G Giacoboni, G H Sguazza, R L de la Sota, M A Stornelli
The aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2...
February 18, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29455477/-application-value-of-xpert-mtb-rif-in-diagnosis-of-spinal-tuberculosis-and-detection-of-rifampin-resistance
#4
Yang-Hui Jin, Shi-Yuan Shi, Qi Zheng, Jian Shen, Xiao-Zhang Ying, Yi-Fan Wang
OBJECTIVE: To investigate the application value of Xpert MTB/RIF in diagnosis of spinal tuberculosis and detection of rifampin resistance. METHODS: The 109 pus specimens were obtained from patients who were primaryly diagnosed as spinal tuberculosis. All of the pus specimens were detected by acid-fast stain, liquid fast culturing by BACTEC MGIT 960 and Xpert MTB/RIF assay to definite the differences in sensitivity and specificity of mycobacterium tuberculosis among detecting methods...
September 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29454739/spanish-and-catalan-translation-cultural-adaptation-and-validation-of-the-picker-patient-experience-questionnaire-15
#5
M J Bertran, M Viñarás, M Salamero, F Garcia, C Graham, A McCulloch, J Escarrabill
OBJECTIVE: To develop and test a culturally adapted core set of questions to measure patients' experience after in-patient care. MATERIAL AND METHODS: Following the methodology recommended by international guides, a basic set of patient experience questions, selected from Picker Institute Europe questionnaires (originally in English), was translated to Spanish and Catalan. Acceptability, construct validity and reliability of the adapted questionnaire were assessed via a cross-sectional validation study...
February 14, 2018: Revista de Calidad Asistencial: Organo de la Sociedad Española de Calidad Asistencial
https://www.readbyqxmd.com/read/29452993/an-in-vitro-evaluation-of-the-efficacy-of-tedizolid-implications-for-the-treatment-of-skin-and-soft-tissue-infections
#6
Pierre Delpech, Muna ALeryan, Brian Jones, Curtis Gemmell, Sue Lang
Skin and soft tissue infections (SSTI) are among the most commonly occurring infections and evidence suggests that these are increasing world-wide. The aetiology is diverse, but Staphylococcus aureus predominate and these are often resistant to antimicrobials that were previously effective. Tedizolid is a new oxazolidinone-class antibacterial indicated for the treatment of adults with SSTI caused by Gram-positive pathogens, including S. aureus. The aim of this study was to evaluate the in vitro efficacy of tedizolid in comparison to other clinically used antibacterials against antibiotic sensitive- and resistant-staphylococci, grown in planktonic cultures and as biofilms reflecting the growth of the microorganism during episodes of SSTI...
January 10, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29451598/performance-of-cryptococcal-antigen-lateral-flow-assay-in-serum-cerebrospinal-fluid-whole-blood-and-urine-in-hiv-infected-patients-with-culture-proven-cryptococcal-meningitis-admitted-at-a-brazilian-referral-center
#7
José E Vidal, Carolina Toniolo, Adriana Paulino, Arnaldo L Colombo, Marilena Dos Anjos Martins, Cristina da Silva Meira, Renata Guise Soares Azevedo, Vera Lucia Pereira-Chioccola, Hélio Rodrigues Gomes, Marcia Dos Santos Lazera, Augusto C Penalva de Oliveira, David R Boulware
Cryptococcal meningitis is the most common cause of opportunistic meningitis in HIV-infected patients in Brazil and causes unacceptable high mortality rates. In this study, HIV-infected patients with a first episode of culture-proven cryptococcal meningitis in cerebrospinal fluid (CSF) were prospectively included in order to evaluate sensitivity of cryptococcal antigen (CrAg) lateral flow assay (LFA) in serum, CSF, whole blood (fingerstick), and fresh urine. In addition, HIV-infected patients with other neurological confirmed diseases were included in order to evaluate the specificity of CrAg LFA in serum...
2018: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/29448067/antiviral-resistance-due-to-deletion-in-the-neuraminidase-gene-and-defective-interfering-like-viral-polymerase-basic-2-rna-of-influenza-a-virus-subtype-h3n2
#8
Ramona Trebbien, Claus Bohn Christiansen, Thea Kølsen Fischer
BACKGROUND AND OBJECTIVE: Antiviral treatment of influenza virus infections can lead to drug resistance of virus. This study investigates a selection of mutations in the full genome of H3N2 influenza A virus isolated from a patient in treatment with oseltamivir. STUDY DESIGN: Respiratory samples from a patient were collected before, during, and after antiviral treatment. Whole genome sequencing of the influenza virus by next generation sequencing, and low-frequency-variant analysis was performed...
February 11, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29447961/a-physiologically-relevant-3d-collagen-based-scaffold-neuroblastoma-cell-system-exhibits-chemosensitivity-similar-to-orthotopic-xenograft-models
#9
C Curtin, J C Nolan, R Conlon, L Deneweth, C Gallagher, Y J Tan, B L Cavanagh, A Z Asraf, H Harvey, S Miller-Delaney, J Shohet, I Bray, F J O'Brien, R L Stallings, O Piskareva
3D scaffold-based in vitro cell culturing is a recent technological advancement in cancer research bridging the gap between conventional 2D culture and in vivo tumours. The main challenge in treating neuroblastoma, a paediatric cancer of the sympathetic nervous system, is to combat tumour metastasis and resistance to multiple chemotherapeutic drugs. The aim of this study was to establish a physiologically relevant 3D neuroblastoma tissue-engineered system and explore its therapeutic relevance. Two neuroblastoma cell lines, chemotherapeutic sensitive Kelly and chemotherapeutic resistant KellyCis83 were cultured in a 3D in vitro model on two collagen-based scaffolds containing either glycosaminoglycan (Coll-GAG) or nanohydroxyapatite (Coll-nHA) and compared to 2D cell culture and an orthotopic murine model...
February 12, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29445355/plasma-levels-of-myonectin-but-not-myostatin-or-fibroblast-derived-growth-factor-21-are-associated-with-insulin-resistance-in-adult-humans-without-diabetes-mellitus
#10
Freddy J K Toloza, Jose O Mantilla-Rivas, Maria C Pérez-Matos, Maria L Ricardo-Silgado, Martha C Morales-Alvarez, Jairo A Pinzón-Cortés, Maritza Pérez-Mayorga, Martha L Arévalo-Garcia, Giovanni Tolosa-González, Carlos O Mendivil
Background: Myokines are a group of protein mediators produced by skeletal muscle under stress or physical exertion. Even though their discovery and effects in cell culture and animal models of disease have elicited great enthusiasm, very little is known about their role in human metabolism. We assessed whether plasma concentrations of three known myokines [myonectin, myostatin, and fibroblast-derived growth factor 21 (FGF-21)] would be associated with direct and indirect indicators of insulin resistance (IR) in individuals who did not have a diagnosis of diabetes...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29444834/accuracy-of-high-throughput-nanofluidic-pcr-based-pneumococcal-serotyping-and-quantification-assays-using-sputum-samples-for-diagnosing-vaccine-serotype-pneumococcal-pneumonia-analyses-by-composite-diagnostic-standards-and-bayesian-latent-class-models
#11
Satoshi Kakiuchi, Motoi Suzuki, Bhim Gopal Dhoubhadel, Akitsugu Furumoto, Hiroyuki Ito, Kei Matsuki, Yoshiko Tsuchihashi, Norichika Asoh, Michio Yasunami, Koya Ariyoshi, Konosuke Morimoto
The lack of reliable diagnostic tests for detecting vaccine serotype pneumococcal pneumonia (VTPP) remains a challenging issue in pneumococcal vaccine studies. This study assessed the performances of high-throughput nanofluidic PCR-based pneumococcal serotyping and quantification assay methods using sputum samples (nanofluidic Sp-qPCR) to diagnose the 13-valent pneumococcal conjugate VTPP compared with that of the serotype-specific urinary antigen detection (UAD) assay using urine samples. Adult pneumonia patients from Japan were enrolled in this study between September 2012 and August 2014...
February 14, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29444749/microbiological-diagnosis-of-adult-tuberculous-meningitis-in-a-ten-year-cohort-in-indonesia
#12
Lidya Chaidir, Jessi Annisa, Sofiati Dian, Ida Parwati, Arlisa Alisjahbana, Feby Purnama, Adri van der Zanden, Ahmad Rizal Ganiem, Reinout van Crevel
We evaluated microbiological diagnosis of tuberculous (TB) meningitis in a referral hospital in Indonesia. Over a ten-year period, we examined cerebrospinal fluid (CSF) samples of 1180 adult meningitis suspects. Sensitivity of different methods was compared, and results were stratified for HIV status, disease severity, and CSF volume. TB meningitis was bacteriologically confirmed in 501 patients. Using clinical diagnosis as reference standard (n = 713), sensitivity of different methods was 12.2% (86/703) for microscopy, 42% (73/174) for Xpert MTB/RIF, 46...
January 9, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29444723/evaluation-of-the-xpert-mtb-rif-performance-on-tissues-potential-impact-on-airborne-infection-isolation-at-a-tertiary-cancer-care-center
#13
Tracy McMillen, Shauna C Usiak, Liang Hua Chen, Luz Gomez, Peter Ntiamoah, Meera R Hameed, Indre Budvytiene, Niaz Banaei, Mini Kamboj, N Esther Babady
OBJECTIVES In this study, we sought to evaluate the performance of the Xpert MTB/RIF (Cepheid) assay for the detection of Mycobacterium tuberculosis (MTB) complex DNA on fresh and formalin-fixed, paraffin-embedded (FFPE) tissue specimens from oncology patients in an area with a low prevalence of tuberculosis. We also aimed to retrospectively assess the potential impact of Xpert MTB/RIF on the duration of airborne infection isolation (AII). SETTING A 473-bed, tertiary-care cancer center in New York City. DESIGN A total of 203 tissue samples (101 FFPE and 102 fresh) were tested using Xpert MTB/RIF, including 133 pulmonary tissue samples (65...
February 15, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29444148/development-of-a-recombinase-polymerase-amplification-assay-for-rapid-detection-of-francisella-noatunensis-subsp-orientalis
#14
Khalid Shahin, Jose Gustavo Ramirez-Paredes, Graham Harold, Benjamin Lopez-Jimena, Alexandra Adams, Manfred Weidmann
Francisella noatunensis subsp. orientalis (Fno) is the causative agent of piscine francisellosis in warm water fish including tilapia. The disease induces chronic granulomatous inflammation with high morbidity and can result in high mortality. Early and accurate detection of Fno is crucial to set appropriate outbreak control measures in tilapia farms. Laboratory detection of Fno mainly depends on bacterial culture and molecular techniques. Recombinase polymerase amplification (RPA) is a novel isothermal technology that has been widely used for the molecular diagnosis of various infectious diseases...
2018: PloS One
https://www.readbyqxmd.com/read/29439270/should-we-use-sentence-or-text-level-tasks-to-measure-oral-language-proficiency-in-year-one-students-following-whole-class-intervention
#15
Maria Lennox, Marleen F Westerveld, David Trembath
AIMS: To compare students' oral language proficiency on sentence- versus text-level tasks at school entry and following tier 1 intervention in their first year of formal schooling. METHODS: 104 students participated in this study. Participants were part of a broader longitudinal study and were enrolled at 3 low socioeconomic, linguistically diverse Australian primary schools. Tasks were administered to all students at the beginning and end of the school year. Performance on the sentence-level task, the Renfrew Action Picture Test (RAPT), was analysed for information and grammar as per the test manual...
February 13, 2018: Folia Phoniatrica et Logopaedica
https://www.readbyqxmd.com/read/29438536/evaluation-of-xpert-mtb-rif-assay-for-diagnosis-of-tuberculosis-in-children
#16
Arghya Das, Shampa Anupurba, Om P Mishra, Tuhina Banerjee, Rajneesh Tripathi
Introduction: Childhood tuberculosis (TB) is now a global priority. With the advent of Xpert MTB/RIF, more TB cases in children are being reported. This study was undertaken to evaluate the performance of Xpert in diagnosis of pulmonary and extra-pulmonary TB in children. Methods: Specimens from 171 suspected TB cases in children aged <15 years were tested with Xpert, culture and smear microscopy in the Department of Microbiology, Institute of Medical Sciences, India...
February 9, 2018: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/29438475/detecting-infections-rapidly-and-easily-for-candidemia-trial-part-2-direct2-a-prospective-multicenter-study-of-the-t2candida-panel
#17
Cornelius J Clancy, Peter G Pappas, Jose Vazquez, Marc A Judson, Dimitrios P Kontoyiannis, George R Thompson, Kevin W Garey, Annette Reboli, Richard N Greenberg, Senu Apewokin, G Marshall Lyon, Luis Ostrosky-Zeichner, Alan H B Wu, Ellis Tobin, M Hong Nguyen, Angela M Caliendo
Background: Blood cultures are approximately 50% sensitive for diagnosing invasive candidiasis. The T2Candida nanodiagnostic panel uses T2 magnetic resonance and a dedicated instrument to detect Candida directly within whole blood samples. Methods: Patients with Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, or Candida krusei candidemia were identified at 14 centers using diagnostic blood cultures (dBCs). Follow-up blood samples were collected concurrently for testing by T2Candida and companion cultures (cBCs)...
February 9, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29438432/cross-detection-for-odor-of-metabolic-waste-between-breast-and-colorectal-cancer-using-canine-olfaction
#18
In-Seok Seo, Hwan-Gon Lee, Bonkon Koo, Chin Su Koh, Hae-Yong Park, Changkyun Im, Hyung-Cheul Shin
Although several studies have been performed to detect cancer using canine olfaction, none have investigated whether canine olfaction trained to the specific odor of one cancer is able to detect odor related to other unfamiliar cancers. To resolve this issue, we employed breast and colorectal cancer in vitro, and investigated whether trained dogs to odor related to metabolic waste from breast cancer are able to detect it from colorectal cancer, and vice versa. The culture liquid samples used in the cultivation of cancerous cells (4T1 and CT26) were employed as an experimental group...
2018: PloS One
https://www.readbyqxmd.com/read/29436623/development-of-uracil-dna-glycosylase-supplemented-loop-mediated-isothermal-amplification-coupled-with-nanogold-probe-udg-lamp-aunp-for-specific-detection-of-pseudomonas%C3%A2-aeruginosa
#19
Orapan Manajit, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
Pseudomonas aeruginosa (P. aeruginosa) is an important opportunistic pathogen that causes serious infections in humans, including keratitis in contact lens wearers. Therefore, establishing a rapid, specific and sensitive method for the identification of P. aeruginosa is imperative. In the present study, the uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification combined with nanogold labeled hybridization probe (UDG-LAMP-AuNP) was developed for the detection of P. aeruginosa. UDG-LAMP was performed to prevent carry over contamination and the LAMP reactions can be readily observed using the nanogold probe...
February 2, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29436423/evaluation-of-the-diagnostic-accuracy-of-2-immunochromatographic-tests-detecting-campylobacter-in-stools-and-their-role-in-campylobacter-infection-diagnosis
#20
Emilie Bessède, Julien Asselineau, Paul Perez, Guillaume Valdenaire, Olivier Richer, Philippe Lehours, Francis Mégraud
The detection of campylobacters in stools is performed essentially by culture, but this technique has a low sensitivity. New detection methods are now available. Among them, immunochromatography tests (ICTs) are very attractive in that they offer a result within 15 minutes. However, previous studies suggest that these tests have a relatively low specificity. The objective of this study was to evaluate the performance of these tests.During the study period, all patients who consulted the emergency units and had a stool culture were included...
February 7, 2018: Journal of Clinical Microbiology
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