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https://www.readbyqxmd.com/read/29665574/diagnostic-methods-to-detect-uterus-illnesses-in-mares
#1
Miguel Alejandro Silva Rua, Celia Raquel Quirino, Rachel Bittencourt Ribeiro, Eulogio Carlos Queiroz Carvalho, Maria de Lourdes Amaral Bernadino, Aylton Bartholazzi Junior, Luisa Fernanda Cipagalta, Marcus Antônio Pessanha Barreto
Uterine illnesses are the major problem in horse reproductive programs. The diagnosis of these pathologies is often neglected or does not provide enough information about the cause. The aim of this study was to evaluate different diagnoses to provide more accurate information about endometritis and the real endometrial profile at the beginning of a breeding season. Fifty-one mares with reproductive failure were evaluated. Two different uterine cytology diagnoses, culture of uterine flush material and biopsy assay, were performed...
April 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29664175/evaluation-of-the-value-of-staining-hair-samples-with-a-modified-wright-giemsa-stain-and-or-showing-illustrated-guidelines-for-the-microscopic-diagnosis-of-dermatophytosis-in-cats
#2
Rareş Căpitan, Carlo Schievano, Chiara Noli
BACKGROUND: Direct examination of the hair is a simple diagnostic test for the diagnosis of dermatophytosis; training is needed to use this test. HYPOTHESIS/OBJECTIVES: To evaluate whether use of modified Wright-Giemsa blue stain and/or photographic images of infected and uninfected hairs improved the user's ability to identify infected or uninfected hairs. ANIMALS: Ten cats with, and 10 cats without, dermatophytosis due to Microsporum canis (n = 20)...
April 17, 2018: Veterinary Dermatology
https://www.readbyqxmd.com/read/29661749/a-website-supporting-sensitive-religious-and-cultural-advance-care-planning-acptalk-formative-and-summative-evaluation
#3
Amanda Pereira-Salgado, Patrick Mader, Clare O'Callaghan, Leanne Boyd
BACKGROUND: Advance care planning (ACP) promotes conversations about future health care needs, enacted if a person is incapable of making decisions at end-of-life that may be communicated through written documentation such as advance care directives. To meet the needs of multicultural and multifaith populations in Australia, an advance care planning website, ACPTalk, was funded to support health professionals in conducting conversations within diverse religious and cultural populations...
April 16, 2018: JMIR Research Protocols
https://www.readbyqxmd.com/read/29660347/detection-and-quantification-of-shrimp-hemocyte-iridescent-virus-by-taqman-probe-based-real-time-pcr
#4
Liang Qiu, Meng-Meng Chen, Xiao-Yuan Wan, Qing-Li Zhang, Chen Li, Xuan Dong, Bing Yang, Jie Huang
Shrimp hemocyte iridescent virus (SHIV) is a recently reported shrimp virus, which threats the cultured white-leg shrimp Litopenaeus vannamei and can cause huge economic loss in shrimp farming industry in China. A quantitative real time polymerase chain reactio (qPCR) assay was developed using a TaqMan probe to detect and quantify SHIV. A pair of qPCR primers, which amplify a 188 bp DNA fragment, and a TaqMan probe were selected from ATPase gene (ORF114R) of the SHIV genome. The primers and TaqMan probe used in this assay were shown to be specific for SHIV and did not react with White spot syndrome virus (WSSV), Infectious hypodermal and hematopoietic necrosis virus (IHHNV), Hepatopancreatic parvovirus (HPV), Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (VPAHPND ), and Enterocytozoon hepatopenaei (EHP), or healthy shrimp DNA...
April 13, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29657404/comparison-of-four-polymerase-chain-reaction-assays-for-the-detection-of-brucella-spp-in-clinical-samples-from-dogs
#5
Eduardo J Boeri, María M Wanke, María J Madariaga, María L Teijeiro, Sebastian A Elena, Marcos D Trangoni
Aim: This study aimed to compare the sensitivity (S), specificity (Sp), and positive likelihood ratios (LR+) of four polymerase chain reaction (PCR) assays for the detection of Brucella spp. in dog's clinical samples. Materials and Methods: A total of 595 samples of whole blood, urine, and genital fluids were evaluated between October 2014 and November 2016. To compare PCR assays, the gold standard was defined using a combination of different serological and microbiological test...
February 2018: Veterinary World
https://www.readbyqxmd.com/read/29656490/cross-cultural-adaptation-reliability-and-validity-of-the-spanish-version-of-the-neonatal-skin-risk-assessment-scale
#6
Pablo García-Molina, Evelin Balaguer López, José Verdú, Andreu Nolasco, Francisco P García Fernández
AIM: To adapt the Neonatal Skin Risk Assessment Scale to the Spanish context and to test its validity and reliability. BACKGROUND: Currently, in Spain there are no validated scales to evaluate the risk of pressure ulcers in infants. METHOD: The research was performed in 10 neonatal units. Overall, we use an observational study design, but divided it in to three stages. In the first stage, the transcultural adaptation of the scale and its content validation was performed...
April 14, 2018: Journal of Nursing Management
https://www.readbyqxmd.com/read/29656268/the-development-and-validation-of-a-short-version-of-the-33-item-hypomania-checklist-hcl-33
#7
Yuan-Yuan Wang, Yuan Feng, Dan-Dan Xu, Wei Huang, Ines H I Chow, Chee H Ng, Gabor S Ungvari, Zhen Yuan, Gang Wang, Yu-Tao Xiang
BACKGROUND: The misdiagnosis of bipolar disorder (BD) as major depressive disorder (MDD) is common in clinical practice and may result in inappropriate treatment. The 33-item Hypomania Checklist (HCL-33) is a newly developed screening scale for BD with satisfactory psychometric properties. In this study, a short version of the HCL-33 was developed and validated to discriminate between BD and MDD. METHOD: All interviews were conducted in a major psychiatric hospital in China...
April 3, 2018: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29655906/substance-use-and-sex-index-susi-first-stage-development-of-an-assessment-tool-to-measure-behaviour-change-in-sexualised-drug-use-for-substance-use-treatment-studies
#8
Nadine Ezard, Beatrice Webb, Brendan Clifford, Michael E Cecilio, Amanda Jellie, Toby Lea, Craig Rodgers, Simon Ruth, Raimondo Bruno
BACKGROUND: Existing tools for measuring blood-borne virus (BBV) and sexually transmitted infection (STI) transmission risk behaviours in substance use interventions have limited capacity to assess risk behaviours across varied social, cultural and epidemiological contexts; have not evolved alongside HIV treatment and prevention innovations; or accounted for sexual contexts of drug use including among a range of lesbian, gay, bisexual, transgender, intersex and queer (LGBTIQ) sub-communities...
April 11, 2018: International Journal on Drug Policy
https://www.readbyqxmd.com/read/29653190/evaluation-of-whole-genome-sequencing-and-software-tools-for-drug-susceptibility-testing-of-mycobacterium-tuberculosis
#9
Janko van Beek, Marjo Haanperä, Pieter W Smit, Silja Mentula, Hanna Soini
OBJECTIVES: Culture-based assays are currently the gold standard for drug susceptibility testing (DST) for Mycobacterium tuberculosis. They provide good sensitivity and specificity, but are time-consuming. The objective of this study was to evaluate whether whole genome sequencing (WGS), combined with software tools for data analysis, can replace routine culture based assays for drug susceptibility testing of M. tuberculosis. METHODS: M. tuberculosis cultures sent to the Finnish mycobacterial reference laboratory in 2014 (n=211) were phenotypically tested by Mycobacteria Growth Indicator Tube (MGIT) for first line drug susceptibilities...
April 10, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29652945/clinical-evaluation-and-validation-of-laboratory-methods-for-the-diagnosis-of-bordetella-pertussis-infection-culture-polymerase-chain-reaction-pcr-and-anti-pertussis-toxin-igg-serology-igg-pt
#10
Adria D Lee, Pamela K Cassiday, Lucia C Pawloski, Kathleen M Tatti, Monte D Martin, Elizabeth C Briere, M Lucia Tondella, Stacey W Martin
INTRODUCTION: The appropriate use of clinically accurate diagnostic tests is essential for the detection of pertussis, a poorly controlled vaccine-preventable disease. The purpose of this study was to estimate the sensitivity and specificity of different diagnostic criteria including culture, multi-target polymerase chain reaction (PCR), anti-pertussis toxin IgG (IgG-PT) serology, and the use of a clinical case definition. An additional objective was to describe the optimal timing of specimen collection for the various tests...
2018: PloS One
https://www.readbyqxmd.com/read/29652111/actual-diagnosis-of-helicobacter-pylori-infection
#11
Philippe Lehours
Helicobacter pylori colonizes the stomach of half of humanity. The prevalence of this infection is higher among people of low socioeconomic status and from developing countries. In the absence of effective antibiotic treatment, the infection persists decades or even lifetime. The diagnosis of H. pylori can be made by invasive methods when biopsies are obtained: culture, histological examination, polymerase chain reaction or rapid urease test. Non-invasive methods have been developed, including the urea breath test, a test of high reliability, particularly suitable for post treatment monitoring, but also the fecal antigen test, as well as the detection of antibodies in the serum...
April 12, 2018: Minerva Gastroenterologica e Dietologica
https://www.readbyqxmd.com/read/29651383/influence-of-encoding-instructions-and-response-bias-on-cross-cultural-differences-in-specific-recognition
#12
Laura E Paige, Selen Amado, Angela H Gutchess
Prior cross-cultural research has reported cultural variations in memory. One study revealed that Americans remembered images with more perceptual detail than East Asians (Millar et al. in Cult Brain 1(2-4):138-157, 2013). However, in a later study, this expected pattern was not replicated, possibly due to differences in encoding instructions (Paige et al. in Cortex 91:250-261, 2017). The present study sought to examine when cultural variation in memory-related decisions occur and the role of instructions. American and East Asian participants viewed images of objects while making a Purchase decision or an Approach decision and later completed a surprise recognition test...
October 2017: Culture and Brain
https://www.readbyqxmd.com/read/29643854/the-role-of-quinine-responsive-taste-receptor-family-2-in-airway-immune-defense-and-chronic-rhinosinusitis
#13
Alan D Workman, Ivy W Maina, Steven G Brooks, Michael A Kohanski, Beverly J Cowart, Corrine Mansfield, David W Kennedy, James N Palmer, Nithin D Adappa, Danielle R Reed, Robert J Lee, Noam A Cohen
Background: Bitter (T2R) and sweet (T1R) taste receptors in the airway are important in innate immune defense, and variations in taste receptor functionality in one T2R (T2R38) correlate with disease status and disease severity in chronic rhinosinusitis (CRS). Quinine is a bitter compound that is an agonist for several T2Rs also expressed on sinonasal cells, but not for T2R38. Because of this property, quinine may stimulate innate immune defense mechanisms in the airway, and functional differences in quinine perception may be reflective of disease status in CRS...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29643799/optical-coherence-tomography-examination-of-the-anterior-segment-in-a-case-of-corneal-perforation-and-lens-trauma-by-chestnut-burr
#14
Takashi Ono, Yosai Mori, Ryohei Nejima, Takuya Iwasaki, Shiro Amano, Kazunori Miyata
Chestnut burrs, the thorny encapsulation of chestnut fruit, can sometimes cause corneal injuries and ulceration, with poor prognoses. We report a case of corneal perforation and damaged anterior lens capsule due to a chestnut burr, using anterior segment optical coherence tomography (AS-OCT). A 67-year-old woman with a chestnut burr injury in her right eye was referred to our hospital. Her right best-corrected visual acuity (BCVA) was 0.8. Slit-lamp examination and AS-OCT showed perforation involving the endothelial layer at the center of the cornea...
January 2018: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/29643202/comparison-of-the-diagnostic-accuracy-of-periprosthetic-tissue-culture-in-blood-culture-bottles-to-prosthesis-sonication-fluid-culture-for-diagnosis-of-prosthetic-joint-infection-using-bayesian-latent-class-modeling-and-idsa-pji-criteria-for-classification
#15
Qun Yan, Melissa J Karau, Kerryl E Greenwood-Quaintance, Jayawant N Mandrekar, Douglas Osmon, Matthew P Abdel, Robin Patel
We have previously demonstrated that culturing periprosthetic tissue in blood culture bottles (BCBs) improves sensitivity compared to conventional agar and broth culture methods for diagnosis of prosthetic joint infection (PJI). We have also shown that prosthesis sonication culture improves sensitivity compared to periprosthetic tissue culture using conventional agar and broth methods. The purpose of this study was to compare the diagnostic accuracy of tissue culture in BCBs (subsequently referred to as tissue culture) to prostheses sonication culture (subsequently referred to as sonicate fluid culture)...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29643198/development-of-a-real-time-rt-pcr-assay-for-the-global-differentiation-of-yellow-fever-virus-vaccine-adverse-events-from-natural-infections
#16
Holly R Hughes, Brandy J Russell, Eric C Mossel, John Kayiwa, Julius Lutwama, Amy J Lambert
Yellow fever (YF) is a reemerging public health threat with frequent outbreaks prompting large vaccination campaigns in endemic regions of Africa and South America. Specific detection of vaccine-related adverse events is resource-intensive, time-consuming and difficult to achieve during an outbreak. To address this, we have developed a highly transferable, rapid yellow fever virus (YFV) vaccine-specific real-time RT-PCR assay that distinguishes vaccine from wild-type lineages. The assay utilizes a specific hydrolysis probe that includes locked nucleic acids to enhance specific discrimination of the YFV 17D vaccine strain genome...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29643195/diagnostic-performance-of-a-molecular-test-versus-clinician-assessment-of-vaginitis
#17
Jane R Schwebke, Charlotte A Gaydos, Paul Nyirjesy, Sonia Paradis, Salma Kodsi, Charles K Cooper
Background: Vaginitis is a common complaint, diagnosed either empirically or using Amsel's criteria and wet mount microscopy. Objective: To determine characteristics of an investigational test (a molecular-based test for vaginitis), as compared to reference, for detection of bacterial vaginosis, Candida spp., and Trichomonas vaginalis Study Design: Vaginal specimens from a cross-sectional study were obtained from 1,740 women (≥18 years old), with vaginitis symptoms, during routine clinic visits (across 10 sites in the USA)...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29642914/development-of-the-italian-version-of-the-high-activity-arthroplasty-score-haas-i-following-hip-and-knee-total-arthroplasty-cross-cultural-adaptation-reliability-validity-and-sensitivity-to-change
#18
Marco Monticone, Antonio Capone, Luca Frigau, Giuseppe Marongiu, Paola Abelli, Francesco Mola, Nicola Maffulli, Calogero Foti
BACKGROUND: The number of physically active individuals who develop knee and hip arthritis and who undergo arthroplasties of these joints ie ever increasing. It has become necessary to develop evaluation scales which address the specific issues raised by such individuals. The High Activity Arthroplasty Score is one such scales, originally developed in English. METHODS: The HAAS-I was developed by means of forward-backward translation, a final review by an expert committee and a test of the pre-final version to establish its correspondence with the original English version...
April 11, 2018: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/29630057/a-simple-method-for-high-throughput-chemical-screening-in-caenorhabditis-elegans
#19
Mark Lucanic, Theo Garrett, Matthew S Gill, Gordon J Lithgow
Caenorhabditis elegans is a useful organism for testing chemical effects on physiology. Whole organism small molecule screens offer significant advantages for identifying biologically active chemical structures that can modify complex phenotypes such as lifespan. Described here is a simple protocol for producing hundreds of 96-well culture plates with fairly consistent numbers of C. elegans in each well. Next, we specified how to use these cultures to screen thousands of chemicals for effects on the lifespan of the nematode C...
March 20, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29628769/antifungal-susceptibility-testing-of-vulvovaginal-candida-species-among-women-attending-antenatal-clinic-in-tertiary-care-hospitals-of-peshawar
#20
Maria Khan, Jawad Ahmed, Amina Gul, Aamer Ikram, Farida Khurram Lalani
Background: Vulvovaginal candidiasis (VVC) is considered as a pervasive gynecological problem among women worldwide. Owing to this fact, in the current study, we aimed at assessing the prevalence rate of Candida spp. causing VVC in symptomatic pregnant women and their antifungal susceptibility pattern. Methods: This study was carried out in the tertiary care hospitals of Peshawar during the period of July 1, 2016 to December 31, 2016. The study group included 450 pregnant women in the age group of 17-44 years with symptoms of excessive vaginal discharge, pain and pruritis...
2018: Infection and Drug Resistance
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