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Influenza hemagglutinin

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https://www.readbyqxmd.com/read/29149637/antiviral-potential-of-natural-compounds-against-influenza-virus-hemagglutinin
#1
S Kannan, P Kolandaivel
Influenza virus of different subtypes H1N1, H2N2, H3N2 and H5N1 cause many human pandemic deaths and threatening the people worldwide. The Hemagglutinin (HA) protein mediates viral attachment to host receptors act as an attractive target. The sixteen natural compounds have been chosen to target the HA protein. Molecular docking studies have been performed to find binding affinity of the compounds. Out of the sixteen, three compounds CI, CII and CIII found to posses a higher binding affinity. The molecular dynamics (MD) simulation has been performed to study the structural, dynamical properties for the nine different complexes CI, CII, CIII bound with H1, H2, H3 proteins and the results were compared...
November 4, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29148388/avian-influenza-h7n9-viruses-co-circulating-among-chickens-southern-china
#2
Nianchen Wang, Minhua Sun, Wenqing Wang, Guowen Ouyang, Zuxian Chen, You Zhang, Bingbing Zhao, Siyu Wu, Jianni Huang, Hailiang Sun, Ming Liao, Peirong Jiao
In April 2017, three avian influenza (H7N9) viruses were isolated from chickens in southern China. Each virus had different insertion points in the cleavage site of the hemagglutinin protein compared to the first identified H7N9 virus. We determined that these viruses were double or triple reassortant viruses.
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29146135/multivalent-oleanolic-acid-human-serum-albumin-conjugate-as-nonglycosylated-neomucin-for-influenza-virus-capture-and-entry-inhibition
#3
Yang Yang, Hao-Jie He, Hao Chang, Yao Yu, Mei-Bing Yang, Yun He, Zhen-Chuan Fan, Suri S Iyer, Peng Yu
We report the synthesis of multivalent oleanolic acid (OA) protein conjugates as nonglycosylated neomucin mimic for the capture and entry inhibition of influenza viruses. Oleanolic acid derivatives bearing an amine-terminated linker were synthesized by esterification of carboxylic acid and further grafted onto the human serum albumin (HSA) via diethyl squarate method. The binding of hemagglutinin (HA) on the virion surface to the synthetic neomucin was evaluated by hemagglutination inhibition assay. The influenza virus capture ability of the PEGylated OA-HSA conjugate was further investigated by Dynamic Light Scattering (DLS), virus capture assay and Isothermal Titration Calorimeter (ITC) techniques...
October 28, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29145498/antigenicity-of-the-2015-2016-seasonal-h1n1-human-influenza-virus-ha-and-na-proteins
#4
Amelia M Clark, Marta L DeDiego, Christopher S Anderson, Jiong Wang, Hongmei Yang, Aitor Nogales, Luis Martinez-Sobrido, Martin S Zand, Mark Y Sangster, David J Topham
Antigenic drift of the hemagglutinin (HA) and neuraminidase (NA) influenza virus proteins contributes to reduced vaccine efficacy. To analyze antigenic drift in human seasonal H1N1 viruses derived from the 2009 pandemic H1N1 virus (pH1N1-like viruses) accounts for the limited effectiveness (around 40%) of vaccination against pH1N1-like viruses during the 2015-2016 season, nasal washes/swabs collected from adult subjects in the Rochester, NY area, were used to sequence and isolate the circulating viruses. The HA and NA proteins from viruses circulating during the 2015-2016 season encoded eighteen and fourteen amino acid differences, respectively, when compared to A/California/04/2009, a strain circulating at the origin of the 2009 pandemic...
2017: PloS One
https://www.readbyqxmd.com/read/29135340/generation-and-testing-anti-influenza-human-monoclonal-antibodies-in-a-new-humanized-mouse-model-draga-hla-a2-hla-dr4-rag1-ko-il-2r%C3%AE-c-ko-nod
#5
Mirian Mendoza, Angela Ballesteros, Qiu Qi, Luis Pow Sang, Soumya Shashikumar, Sofia Casares, Teodor-D Brumeanu
Pandemic outbreaks of influenza type A viruses have resulted in numerous fatalities around the globe. Since the conventional influenza vaccines (CIV) provide less than 20% protection for individuals with weak immune system, it has been considered that broadly cross-neutralizing antibodies may provide a better protection. Herein, we showed that a recently generated humanized mouse (DRAGA mouse; HLA-A2. HLA-DR4. Rag1KO. IL-2Rγc KO. NOD) that lacks the murine immune system and expresses a functional human immune system can be used to generate cross-reactive, human anti-influenza monoclonal antibodies (hu-mAb)...
November 14, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29134458/animal-cell-expression-systems
#6
M Butler, Ing U Reichl
The glycan profile of therapeutic recombinant proteins such as monoclonal antibodies is a critical quality attribute, which affects the efficacy of the final product. The cellular glycosylation process during protein expression is dependent upon a number of factors such as the availability of substrates in the media, the intracellular content of nucleotide sugars, and the enzyme repertoire of the host cells. In order to control the variability of glycosylation it is important to understand the critical process parameters and their acceptable range of values to enable reproducible production of proteins with a predetermined glycan profile providing the desired biological function or therapeutic effect...
October 3, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/29129705/a-virus-like-particle-of-the-hepatitis-b-virus-pres-antigen-elicits-robust-neutralizing-antibody-and-t-cell-responses-in-mice
#7
Xiaodan Cai, Weihao Zheng, Shaokun Pan, Shengyuan Zhang, Youhua Xie, Haitao Guo, Guoxin Wang, Zigang Li, Ming Luo
The preS antigen of hepatitis B virus (HBV) corresponds to the N-terminal polypeptide in the large (L) antigen in addition to the small (S) antigen. The virus-like particle (VLP) of the S antigen is widely used as a vaccine to protect the population from HBV infection. The presence of the S antigen and its antibodies in patient blood has been used as markers to monitor hepatitis B. However, there is very limited knowledge about the preS antigen. We generated a preS VLP that is formed by a chimeric protein between preS and hemagglutinin (HA), and the matrix protein M1 of influenza virus...
November 9, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29128380/low-influenza-vaccine-effectiveness-and-the-effect-of-previous-vaccination-in-preventing-admission-with-a-h1n1-pdm09-or-b-victoria-lineage-in-patients-60-years-old-or-older-during-the-2015-2016-influenza-season
#8
Joan Puig-Barberà, Beatriz Guglieri-López, Miguel Tortajada-Girbés, F Xavier López-Labrador, Mario Carballido-Fernández, Joan Mollar-Maseres, Germán Schwarz-Chavarri, Víctor Baselga-Moreno, Ainara Mira-Iglesias, Javier Díez-Domingo
BACKGROUND: The 2015/2016 influenza season was characterized in Europe by the circulation of A(H1N1)pdm09 clade 6B.1 and B/Victoria-lineage influenza viruses. The components of the vaccines used in the current and past two seasons in the Valencia region were similar but not well matched to the 2015/2016 dominant influenza-circulating strains. We estimate influenza vaccine effectiveness (IVE) and interference of previous vaccination in preventing admission with A(H1N1)pdm09 or B/Victoria-lineage in this particular season...
November 8, 2017: Vaccine
https://www.readbyqxmd.com/read/29127198/probing-the-metastable-state-of-influenza-hemagglutinin
#9
Carolyn N Kingsley, Aleksandar Antanasijevic, Helena Palka-Hamblin, Matthew Durst, Benjamin Ramirez, Arnon Lavie, Michael Caffrey
Viral entry into host cells is mediated by membrane proteins in a metastable state that transition to a more stable state upon a stimulus. For example, in the influenza envelope protein hemagglutinin (HA), the low pH in the endosome triggers a transition from the metastable prefusion conformation to the stable fusion conformation. To identify probes that interfere with HA function, here we screened a library of H7 HA peptides for inhibition of H7 HA-mediated entry. We discovered a peptide, PEP87 (WSYNAELLVAMENQHTI), that inhibited H7 and H5 HA-mediated entry...
November 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29126399/production-and-purification-of-chimeric-hbc-virus-like-particles-carrying-influenza-virus-lah-domain-as-vaccine-candidates
#10
Andris Kazaks, I-Na Lu, Sophie Farinelle, Alex Ramirez, Vincenzo Crescente, Benjamin Blaha, Olotu Ogonah, Tarit Mukhopadhyay, Mapi Perez de Obanos, Alejandro Krimer, Inara Akopjana, Janis Bogans, Velta Ose, Anna Kirsteina, Tatjana Kazaka, Nicola J Stonehouse, David J Rowlands, Claude P Muller, Kaspars Tars, William M Rosenberg
BACKGROUND: The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus strains. The long alpha helix (LAH) is an attractive vaccine component since it is one of the most conserved influenza hemagglutinin (HA) stalk regions. For an improved immune response, the LAH domain from H3N2 strain has been incorporated into virus-like particles (VLPs) derived from hepatitis B virus core protein (HBc) using recently developed tandem core technology...
November 10, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29122283/fast-and-highly-selective-determination-of-hemagglutinin-content-in-quadrivalent-influenza-vaccine-by-reversed-phase-high-performance-liquid-chromatography-method
#11
Barry Lorbetskie, Nathalie Fortin, Laura Durno, Junzhi Wang, Changgui Li, Xuguang Li, Michel Girard, Simon Sauvé
Seasonal inactivated quadrivalent influenza vaccines are currently formulated to include antigens from two strains of influenza A and a strain from each of the two circulating influenza B virus lineages. However, the applicability of the potency assay currently required for the release of vaccines has been hindered due to cross-reactivity between the two B strains. In this study, a reversed-phase high-performance liquid chromatography method previously developed for the separation and quantitative determination of the hemagglutinin content in trivalent influenza vaccine preparations was further extended and found to be adaptable for the assessment of all four hemagglutinin antigens present in quadrivalent influenza vaccines...
October 18, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29119400/comparative-study-of-fusogenic-activity-of-h1-and-h5-subtypes-influenza-virus-hemagglutinins
#12
A A Kononova, S V Cheresiz, A V Chechushkov, Yu V Razumova, A G Pokrovskii
Influenza virus hemagglutinins are surface proteins responsible for fusion of the viral and cellular membranes. Their capacity to mediate membrane fusion (fusogenic activity) is studied by various methods, including the syncytium formation and pseudovirus transduction methods. We constructed plasmids coding for genes of three H1 and one H5 hemagglutinins and compared their fusogenic activities. Hemagglutinin capacity to induce syncytium formation did not always correlate with the transduction activity of the respective pseudoviruses...
November 9, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29118122/unique-directional-motility-of-influenza-c-virus-controlled-by-its-filamentous-morphology-and-short-range-motions
#13
Tatsuya Sakai, Hiroaki Takagi, Yasushi Muraki, Mineki Saito
Influenza virus motility is based on cooperation between two viral spike proteins, hemagglutinin (HA) and neuraminidase (NA), and is a major determinant of virus infectivity. To translocate a virus particle on the cell surface, HA molecules exchange viral receptors and NA molecules accelerate the receptor exchange of HA. This type of virus motility was recently identified in influenza A virus (IAV). To determine if other influenza virus types have a similar receptor-exchange mechanism-driven motility, we investigated influenza C virus (ICV) motility on a receptor-fixed glass surface...
November 8, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29118117/a-y161f-hemagglutinin-substitution-increases-thermostability-and-improves-yields-of-2009-h1n1-influenza-a-virus-in-cells
#14
Feng Wen, Lei Li, Nan Zhao, Meng-Jung Chiang, Hang Xie, Jim Cooley, Richard Webby, Peng George Wang, Xiu-Feng Wan
Vaccination is the primary strategy for influenza prevention and control. Yet egg-based vaccines, the predominant production platform, have several disadvantages including the emergence of viral antigenic variants that can be induced during egg passage. These limitations have prompted development of cell-based vaccines which themselves are not without issue. Most importantly, vaccine seed viruses often do not grow efficiently in mammalian cell lines. Here we aimed to identify novel high-yield signatures for influenza viruses in continuous Madin-Darby canine kidney (MDCK) and Vero cells...
November 8, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29113775/spread-of-predominant-neuraminidase-and-hemagglutinin-co-mutations-in-the-influenza-a-h3n2-virus-genome
#15
Yong Chong, Hideyuki Ikematsu
Genetic variation of influenza neuraminidase (NA), unlike for hemagglutinin (HA), has not been fully characterized. Therefore, we determined the relation between mutations in the NA and HA genome segments of 205 influenza A/H3N2 viruses isolated from patients in Japan during the five seasons from 2010 to 2015. The amino acid (AA) sequences of the NA and HA proteins in these isolates were then determined. In the 2011-2012 season, there was the emergence of isolates with NA and HA sequences containing AA93G (NA93G) and AA278K (HA278K), respectively (24/48 isolates, 50...
November 4, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29112968/fitness-cost-of-reassortment-in-human-influenza
#16
Mara Villa, Michael Lässig
Reassortment, which is the exchange of genome sequence between viruses co-infecting a host cell, plays an important role in the evolution of segmented viruses. In the human influenza virus, reassortment happens most frequently between co-existing variants within the same lineage. This process breaks genetic linkage and fitness correlations between viral genome segments, but the resulting net effect on viral fitness has remained unclear. In this paper, we determine rate and average selective effect of reassortment processes in the human influenza lineage A/H3N2...
November 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29112488/immunogenicity-and-safety-of-a-novel-seasonal-influenza-preservative-free-vaccine-manufactured-in-kazakhstan-results-of-a-comparative-phase-ii-clinical-trial-in-adults
#17
Gulbanu Sarsenbayeva, Yevgeniy Volgin, Markhabat Kassenov, Timur Issagulov, Nikolay Bogdanov, Abylay Sansyzbay, Marina Stukova, Zhanna Buzitskaya, Ilyas Кulmagambetov, Timur Davlyatshin, Berik Khairullin
BACKGROUND: The study was aimed at comparative evaluation of seasonal influenza vaccine RIBSP versus commercial vaccine VAXIGRIP® for immunogenicity and safety in the course of clinical trial phase II on healthy volunteers aged 18-60 years. METHODS: The trial involved 150 subjects in randomized 2:1 groups that received either RIBSP vaccine or comparator vaccine VAXIGRIP®. One dose (0.5 ml) of either vaccine contained 15 μg of hemagglutinin of each influenza virus strain recommended by WHO for the Northern hemisphere in 2016-2017 flu season...
November 7, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29111681/design-of-glycopolymers-carrying-sialyl-oligosaccharides-for-controlling-the-interaction-with-the-influenza-virus
#18
Masanori Nagao, Yurina Fujiwara, Teruhiko Matsubara, Yu Hoshino, Toshinori Sato, Yoshiko Miura
We designed glycopolymers carrying sialyl oligosaccharides by "post-click" chemistry and evaluated the interaction with the influenza virus. The glycopolymer structures were synthesized in a well-controlled manner by reversible addition-fragmentation chain transfer polymerization and the Huisgen reaction. Acrylamide-type monomers were copolymerized to give hydrophilicity to the polymer backbones, and the hydrophilicity enabled the successful introduction of the oligosaccharides into the polymer backbones. The glycopolymers with different sugar densities and polymer lengths were designed for the interaction with hemagglutinin on the virus surface...
November 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29111376/analysis-of-the-proficiency-of-single-radial-immunodiffusion-assays-for-quality-control-of-influenza-vaccines-in-korea
#19
Yejin Choi, Sunmi Lee, Seong Yi Kwon, Younghee Lee, Yong Keun Park, Sang Ja Ban
Influenza vaccine potency, which is determined by quantitatively measuring the content of Hemagglutinin (HA), is an essential index representing the efficacy of the vaccine. Standardization of the single radial immunodiffusion (SRID) assay, a method for measuring HA content, and proficiency of the testing institutions are crucial for influenza vaccine quality control. Herein, we assessed the proficiency of SRID assays at the National Control Laboratory (NCL) of Korea and several vaccine manufacturers. Eight laboratories participated in this study, and the proficiencies of all laboratories yielded satisfactory results in overall SRID assays...
October 27, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29109276/contemporary-h3n2-influenza-viruses-have-a-glycosylation-site-that-alters-binding-of-antibodies-elicited-by-egg-adapted-vaccine-strains
#20
Seth J Zost, Kaela Parkhouse, Megan E Gumina, Kangchon Kim, Sebastian Diaz Perez, Patrick C Wilson, John J Treanor, Andrea J Sant, Sarah Cobey, Scott E Hensley
H3N2 viruses continuously acquire mutations in the hemagglutinin (HA) glycoprotein that abrogate binding of human antibodies. During the 2014-2015 influenza season, clade 3C.2a H3N2 viruses possessing a new predicted glycosylation site in antigenic site B of HA emerged, and these viruses remain prevalent today. The 2016-2017 seasonal influenza vaccine was updated to include a clade 3C.2a H3N2 strain; however, the egg-adapted version of this viral strain lacks the new putative glycosylation site. Here, we biochemically demonstrate that the HA antigenic site B of circulating clade 3C...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
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