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Influenza hemagglutinin

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https://www.readbyqxmd.com/read/28212428/a-novel-hemagglutinin-protein-produced-in-bacteria-protects-chickens-against-h5n1-highly-pathogenic-avian-influenza-viruses-by-inducing-h5-subtype-specific-neutralizing-antibodies
#1
Violetta Sączyńska, Agnieszka Romanik, Katarzyna Florys, Violetta Cecuda-Adamczewska, Małgorzata Kęsik-Brodacka, Krzysztof Śmietanka, Monika Olszewska, Katarzyna Domańska-Blicharz, Zenon Minta, Bogusław Szewczyk, Grażyna Płucienniczak, Andrzej Płucienniczak
The highly pathogenic (HP) H5N1 avian influenza viruses (AIVs) cause a mortality rate of up to 100% in infected chickens and pose a permanent pandemic threat. Attempts to obtain effective vaccines against H5N1 HPAIVs have focused on hemagglutinin (HA), an immunodominant viral antigen capable of eliciting neutralizing antibodies. The vast majority of vaccine projects have been performed using eukaryotic expression systems. In contrast, we used a bacterial expression system to produce vaccine HA protein (bacterial HA) according to our own design...
2017: PloS One
https://www.readbyqxmd.com/read/28210631/immunogenicity-and-cross-protection-in-mice-afforded-by-pandemic-h1n1-live-attenuated-influenza-vaccine-containing-wild-type-nucleoprotein
#2
Andrey Rekstin, Irina Isakova-Sivak, Galina Petukhova, Daniil Korenkov, Igor Losev, Tatiana Smolonogina, Tatiana Tretiak, Svetlana Donina, Svetlana Shcherbik, Tatiana Bousse, Larisa Rudenko
Since conserved viral proteins of influenza virus, such as nucleoprotein (NP) and matrix 1 protein, are the main targets for virus-specific CD8+ cytotoxic T-lymphocytes (CTLs), we hypothesized that introduction of the NP gene of wild-type virus into the genome of vaccine reassortants could lead to better immunogenicity and afford better protection. This paper describes in vitro and in vivo preclinical studies of two new reassortants of pandemic H1N1 live attenuated influenza vaccine (LAIV) candidates. One had the hemagglutinin (HA) and neuraminidase (NA) genes from A/South Africa/3626/2013 H1N1 wild-type virus on the A/Leningrad/134/17/57 master donor virus backbone (6 : 2 formulation) while the second had the HA, NA, and NP genes of the wild-type virus on the same backbone (5 : 3 formulation)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28207986/novel-multiplex-assay-platforms-to-detect-influenza-a-hemagglutinin-subtype-specific-antibody-responses-for-high-throughput-and-in-field-applications
#3
Zhu-Nan Li, Jessica F Trost, Kimberly M Weber, Elizabeth H LeMasters, Sharifa Nasreen, Javan Esfandiari, Angelo H Gunasekera, Megan McCausland, Katharine Sturm-Ramirez, Jens Wrammert, Sean Gregory, Vic Veguilla, James Stevens, Joseph D Miller, Jacqueline M Katz, Min Z Levine
BACKGROUND: Detections of influenza A subtype specific antibody responses are often complicated by the presence of cross-reactive antibodies. We developed two novel multiplex platforms for antibody detection. The multiplexed magnetic fluorescence microsphere immunoassay (MAGPIX) is a high throughput laboratory-based assay. Chembio Dual Path Platform (DPP) is a portable and rapid test that could be used in the field. METHODS: Twelve recombinant globular head domain hemagglutinin (GH HA1) antigens from A(H1N1)pdm09 (pH1N1), A(H2N2), A(H3N2), A(H5N1), A(H7N9), A(H9N2), A(H13N9), B/Victoria lineage, B/Yamagata lineage viruses, and protein A control were used...
February 16, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/28202040/subcellular-localization-and-interactions-of-infectious-salmon-anemia-virus-isav-m1-and-nep-as-well-as-host-hsc70
#4
Wenting Zhang, Chengzhi Cai, Li Lin, Yizhi Jane Tao, Meilin Jin
BACKGROUND: Infectious salmon anemia virus (ISAV) is an important fish pathogen that causes high mortality in farmed Atlantic salmon. The ISAV genome consists of eight single-stranded, negative-sense RNA segments. The six largest segments contain one open reading frame (ORF) each, and encode three polymerase proteins, nucleoprotein, fusion protein, and hemagglutinin esterase protein. The two smallest segments contain more than one ORF each. The segment 7 encodes non-structural protein 1 (NS1) and nuclear export protein (NEP), while segment 8 encodes matrix protein 1 and 2 (M1 and M2)...
February 15, 2017: Virology Journal
https://www.readbyqxmd.com/read/28196963/genetic-predisposition-to-acquire-a-polybasic-cleavage-site-for-highly-pathogenic-avian-influenza-virus-hemagglutinin
#5
Naganori Nao, Junya Yamagishi, Hiroko Miyamoto, Manabu Igarashi, Rashid Manzoor, Aiko Ohnuma, Yoshimi Tsuda, Wakako Furuyama, Asako Shigeno, Masahiro Kajihara, Noriko Kishida, Reiko Yoshida, Ayato Takada
Highly pathogenic avian influenza viruses with H5 and H7 hemagglutinin (HA) subtypes evolve from low-pathogenic precursors through the acquisition of multiple basic amino acid residues at the HA cleavage site. Although this mechanism has been observed to occur naturally only in these HA subtypes, little is known about the genetic basis for the acquisition of the polybasic HA cleavage site. Here we show that consecutive adenine residues and a stem-loop structure, which are frequently found in the viral RNA region encoding amino acids around the cleavage site of low-pathogenic H5 and H7 viruses isolated from waterfowl reservoirs, are important for nucleotide insertions into this RNA region...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28192418/defining-the-antibody-cross-reactome-directed-against-the-influenza-virus-surface-glycoproteins
#6
Raffael Nachbagauer, Angela Choi, Ariana Hirsh, Irina Margine, Sayaka Iida, Aldo Barrera, Marcela Ferres, Randy A Albrecht, Adolfo García-Sastre, Nicole M Bouvier, Kimihito Ito, Rafael A Medina, Peter Palese, Florian Krammer
Infection with influenza virus induces antibodies to the viral surface glycoproteins hemagglutinin and neuraminidase, and these responses can be broadly protective. To assess the breadth and magnitude of antibody responses, we sequentially infected mice, guinea pigs and ferrets with divergent H1N1 or H3N2 subtypes of influenza virus. We measured antibody responses by ELISA of an extensive panel of recombinant glycoproteins representing the viral diversity in nature. Guinea pigs developed high titers of broadly cross-reactive antibodies; mice and ferrets exhibited narrower humoral responses...
February 13, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28192417/defining-b-cell-immunodominance-to-viruses
#7
Davide Angeletti, James S Gibbs, Matthew Angel, Ivan Kosik, Heather D Hickman, Gregory M Frank, Suman R Das, Adam K Wheatley, Madhu Prabhakaran, David J Leggat, Adrian B McDermott, Jonathan W Yewdell
Immunodominance (ID) defines the hierarchical immune response to competing antigens in complex immunogens. Little is known regarding B cell and antibody ID despite its importance in immunity to viruses and other pathogens. We show that B cells and serum antibodies from inbred mice demonstrate a reproducible ID hierarchy to the five major antigenic sites in the influenza A virus hemagglutinin globular domain. The hierarchy changed as the immune response progressed, and it was dependent on antigen formulation and delivery...
February 13, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28191464/construction-of-multilevel-structure-for-avian-influenza-virus-system-based-on-granular-computing
#8
Yang Li, Qi-Hao Liang, Meng-Meng Sun, Xu-Qing Tang, Ping Zhu
Exploring the genetic structure of influenza viruses attracts the attention in the field of molecular ecology and medical genetics, whose epidemics cause morbidity and mortality worldwide. The rapid variations in RNA strand and changes of protein structure of the virus result in low-accuracy subtyping identification and make it difficult to develop effective drugs and vaccine. This paper constructs the evolutionary structure of avian influenza virus system considering both hemagglutinin and neuraminidase protein fragments...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28187952/immunogenicity-and-safety-of-an-as03-adjuvanted-h7n1-vaccine-in-healthy-adults-a-phase-i-ii-observer-blind-randomized-controlled-trial
#9
Anuradha Madan, Murdo Ferguson, Eric Sheldon, Nathan Segall, Laurence Chu, Azhar Toma, Paul Rheault, Damien Friel, Jyoti Soni, Ping Li, Bruce L Innis, Anne Schuind
BACKGROUND: H7 influenza strains have pandemic potential. AS03-adjuvanted H7N1 A/mallard/Netherlands/12/2000 split-virion vaccine formulations were evaluated as model H7-subtype vaccine and tested after H7N9 emerged in China, and caused severe human disease with high mortality. METHODS: In this phase I/II, observer-blind, randomized trial in US and Canada, 420 healthy adults (21-64years) were randomized to receive 1 of 4 H7N1 vaccine formulations (3.75 or 7.5μg hemagglutinin adjuvanted with either AS03A or AS03B), 15μg unadjuvanted H7N1 hemagglutinin, or saline placebo, given as 2-dose series...
February 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28187149/inhibition-of-influenza-a-virus-infection-by-ginsenosides
#10
Wei Dong, Amber Farooqui, Alberto J Leon, David J Kelvin
Influenza viruses cause mild to severe respiratory infections in humans. Due to efficient means of transmission, the viruses infect human population on a large scale. Apart from vaccines, antiviral drugs are used to control infection; neuraminidase inhibitors are thought to be the first choice of treatment, particularly for severe cases. Rapidly evolving and emerging influenza viruses with increased frequency of viral resistance to these drugs stress the need to explore novel antiviral compounds. In this study, we investigated antiviral activity of ginseng extract and ginsenosides, the ginseng-derived triterpene and saponin compounds, against 2009 pandemic H1N1 virus in vitro and in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28181197/circulation-of-antibodies-against-influenza-virus-hemagglutinins-in-the-2014-2015-epidemic-season-in-poland
#11
D Kowalczyk, K Szymański, K Cieślak, L B Brydak
The aim of this study was to determine the level of anti-hemagglutinin antibodies in the serum of people in different age-groups during the 2014/2015 epidemic influenza season in Poland. A total of 1050 sera were tested. The level of anti-hemagglutinin antibodies was determined using the hemagglutinin inhibition test. The results provided information on the incidence of circulating A/California/7/2009(H1N1)pdm09, A/Texas/50/2012(H3N2), and B/Massachusetts/2/2012 viruses. The level of antibodies against influenza differed between age-groups...
February 9, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28179523/progesterone-based-contraceptives-reduce-adaptive-immune-responses-and-protection-against-sequential-influenza-a-virus-infections
#12
Olivia J Hall, Raffael Nachbagauer, Meghan S Vermillion, Ashley L Fink, Vanessa Phuong, Florian Krammer, Sabra L Klein
In addition to their intended use, progesterone (P4)-based contraceptives promote anti-inflammatory immune responses, yet their effects on the outcome of infectious diseases, including influenza A virus (IAV), are rarely evaluated. To evaluate their impact on immune responses to sequential IAV infections, adult female mice were treated with placebo or one of two progestins, P4 or levonorgestrel (LNG), and infected with mouse adapted (ma) H1N1 virus. Treatment with P4 or LNG reduced morbidity, but had no effect on pulmonary virus titers, during primary H1N1 infection as compared to placebo treatment...
February 8, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28168175/preparation-and-immunogenicity-of-influenza-virus-like-particles-using-nitrocellulose-membrane-filtration
#13
Young Chan Park, Jae Min Song
PURPOSE: Nitrocellulose membrane-based filtration system (NCFS) is widely used for protein concentration. In this study, we applied NCFS for production of virus-like particle (VLP) as a vaccine candidate and evaluated yield property and immunogenicity. MATERIALS AND METHODS: Influenza VLPs were generated by baculovirus-insect cell protein expression system. NCFS and sucrose gradient ultracentrifugation were used for purification of VLP. Immunogenicity of VLP was evaluated by animal experiment...
January 2017: Clinical and Experimental Vaccine Research
https://www.readbyqxmd.com/read/28166830/the-t160a-hemagglutinin-substitution-affects-not-only-receptor-binding-property-but-also-transmissibility-of-h5n1-clade-2-3-4-avian-influenza-virus-in-guinea-pigs
#14
Min Gu, Qunhui Li, Ruyi Gao, Dongchang He, Yunpeng Xu, Haixu Xu, Lijun Xu, Xiaoquan Wang, Jiao Hu, Xiaowen Liu, Shunlin Hu, Daxin Peng, Xinan Jiao, Xiufan Liu
We generated and characterized site-directed HA mutants on the genetic backbone of H5N1 clade 2.3.4 virus preferentially binding to α-2,3 receptors in order to identify the key determinants in hemagglutinin rendering the dual affinity to both α-2,3 (avian-type) and α-2,6 (human-type) linked sialic acid receptors of the current clade 2.3.4.4 H5NX subtype avian influenza reassortants. The results show that the T160A substitution resulted in the loss of a glycosylation site at 158N and led not only to enhanced binding specificity for human-type receptors but also transmissibility among guinea pigs, which could be considered as an important molecular marker for assessing pandemic potential of H5 subtype avian influenza isolates...
February 6, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28165025/a-universal-computational-model-for-predicting-antigenic-variants-of-influenza-a-virus-based-on-conserved-antigenic-structures
#15
Yousong Peng, Dayan Wang, Jianhong Wang, Kenli Li, Zhongyang Tan, Yuelong Shu, Taijiao Jiang
Rapid determination of the antigenicity of influenza A virus could help identify the antigenic variants in time. Currently, there is a lack of computational models for predicting antigenic variants of some common hemagglutinin (HA) subtypes of influenza A viruses. By means of sequence analysis, we demonstrate here that multiple HA subtypes of influenza A virus undergo similar mutation patterns of HA1 protein (the immunogenic part of HA). Further analysis on the antigenic variation of influenza A virus H1N1, H3N2 and H5N1 showed that the amino acid residues' contribution to antigenic variation highly differed in these subtypes, while the regional bands, defined based on their distance to the top of HA1, played conserved roles in antigenic variation of these subtypes...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28162820/manipulation-of-neuraminidase-packaging-signals-and-hemagglutinin-residues-improves-the-growth-of-a-anhui-1-2013-h7n9-influenza-vaccine-virus-yield-in-eggs
#16
Subrata Barman, Petr S Krylov, Jasmine C Turner, John Franks, Robert G Webster, Matloob Husain, Richard J Webby
In 2013, a novel avian-origin H7N9 influenza A virus causing severe lower respiratory tract disease in humans emerged in China, with continued sporadic cases. An effective vaccine is needed for this virus in case it acquires transmissibility among humans; however, PR8-based A/Anhui/1/2013 (Anhui/1, H7N9), a WHO-recommended H7N9 candidate vaccine virus (CVV) for vaccine production, does not replicate well in chicken eggs, posing an obstacle to egg-based vaccine production. To address this issue, we explored the possibility that PR8's hemagglutinin (HA) and neuraminidase (NA) packaging signals mediate improvement of Anhui/1 CVV yield in eggs...
February 2, 2017: Vaccine
https://www.readbyqxmd.com/read/28159667/a-novel-method-for-detection-of-h9n2-influenza-viruses-by-an-aptamer-real-time-pcr
#17
Issam Hmila, Manoosak Wongphatcharachai, Nacira Laamiri, Rim Aouini, Boutheina Marnissi, Marwa Arbi, Srinand Sreevatsan, Abdeljelil Ghram
H9N2 Influenza subtype has emerged in Tunisia causing epidemics in poultry and resulting in major economic losses. New mutations in their hemagglutinin and neuraminidase proteins were acquired, suggesting their potential to directly infect humans. Effective surveillance tools should be implemented to help prevent potential spillover of the virus across species. We have developed a highly sensitive real time immuno-polymerase chain reaction (RT-I-PCR) method for detecting H9N2 virus. The assay applies aptamers as ligands to capture and detect the virus...
January 31, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28159612/a-prime-boost-vaccination-with-ha-dna-and-pichia-produced-ha-protein-elicits-a-strong-humoral-response-in-chickens-against-h5n1
#18
Anna Stachyra, Maria Pietrzak, Agnieszka Macioła, Anna Protasiuk, Monika Olszewska, Krzysztof Śmietanka, Zenon Minta, Anna Góra-Sochacka, Edyta Kopera, Agnieszka Sirko
Highly pathogenic avian influenza viruses cause severe disease and huge economic losses in domestic poultry and might pose a serious threat to people because of the high mortality rates in case of an accidental transmission to humans. The main goal of this work was to evaluate the immune responses and hemagglutination inhibition potential elicited by a combined DNA/recombinant protein prime/boost vaccination compared to DNA/DNA and protein/protein regimens in chickens. A plasmid encoding hemagglutinin (HA) from the A/swan/Poland/305-135V08/2006 (H5N1) virus, or the recombinant HA protein produced in Pichia pastoris system, both induced H5 HA-specific humoral immune responses in chickens...
January 31, 2017: Virus Research
https://www.readbyqxmd.com/read/28158271/genetic-and-antigenic-evolution-of-h9n2-subtype-avian-influenza-virus-in-domestic-chickens-in-southwestern-china-2013-2016
#19
Jing Xia, Jia-Qi Cui, Xiao He, Yue-Yue Liu, Ke-Chang Yao, San-Jie Cao, Xin-Feng Han, Yong Huang
H9N2 avian influenza virus (AIV) has caused significant losses in chicken flocks throughout china in recent years. There is a limited understanding of the genetic and antigenic characteristics of the H9N2 virus isolated in chickens in southwestern China. In this study a total of 12 field strains were isolated from tissue samples from diseased chickens between 2013 and 2016. Phylogenetic analysis of the Hemagglutinin (HA) and Neuraminidase (NA) nucleotide sequences from the 12 field isolates and other reference strains showed that most of the isolates in the past four years could be clustered into a major branch (HA-branch A and NA-branch I) in the Clade h9...
2017: PloS One
https://www.readbyqxmd.com/read/28157547/cell-cultured-live-attenuated-x-31ca-based-h5n1-pre-pandemic-influenza-vaccine
#20
Yun Ha Lee, Yo Han Jang, Baik Lin Seong
The manufacture of influenza vaccines has traditionally depended upon a method using embryonated hen's eggs. However, concerns regarding the potential shortage of the influenza substrate in the event of a pandemic has led to the development of cell culture-derived vaccines, which offers shorter lead-in times and greater production flexibility. We examined optimal conditions for the production of reassortant X-31ca-based H5N1 cold-adapted live attenuated influenza vaccine (rH5N1ca) cultured in mammalian cell lines...
January 31, 2017: Virology
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