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Influenza hemagglutinin

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https://www.readbyqxmd.com/read/28531130/comparative-safety-and-efficacy-profile-of-a-novel-oil-in-water-vaccine-adjuvant-comprising-vitamins-a-and-e-and-a-catechin-in-protective-anti-influenza-immunity
#1
Sapna Patel, Yasser Faraj, Debra K Duso, William W Reiley, Erik A Karlsson, Stacey Schultz-Cherry, Michael Vajdy
Non-replicating vaccines, such as those based on recombinant proteins, require adjuvants and delivery systems, which have thus far depended on mimicking pathogen danger signals and strong pro-inflammatory responses. In search of a safer and more efficacious alternative, we tested whether vaccinations with influenza recombinant hemagglutinin (HA) mixed with a novel vegetable oil in water emulsion adjuvant (Natural Immune-enhancing Delivery System, NIDS), based on the immune-enhancing synergy of vitamins A and E and a catechin, could protect against intra-nasal challenge with live influenza virus...
May 21, 2017: Nutrients
https://www.readbyqxmd.com/read/28514082/universal-label-free-in-process-quantification-of-influenza-virus-like-particles
#2
Sofia B Carvalho, Mafalda G Moleirinho, David Wheatley, John Welsh, René Gantier, Paula M Alves, Cristina Peixoto, Manuel J T Carrondo
Virus-like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess. However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress. Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion. These analytical technologies are time-consuming, cumbersome, and not supportive of efficient downstream process development and monitoring...
May 17, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28513329/serologic-detection-of-subtype-specific-antibodies-to-influenza-a-viruses-in-southern-sea-otters-enhydra-lutris-nereis
#3
Alyssa M Capuano, Melissa Miller, David E Stallknecht, Megan Moriarty, Magdalena Plancarte, Erin Dodd, Francesca Batac, Walter M Boyce
There are approximately 3,000 southern sea otters (Enhydra lutris nereis) in the nearshore environment along the California coast, and the species is classified as Threatened under the Endangered Species Act. We tested sera from 661 necropsied southern sea otters sampled from 1997 to 2015 to determine overall exposure to influenza A viruses (IAVs) and to identify subtype-specific antibody responses. Using an enzyme-linked immunosorbent assay (ELISA), antibodies to IAV nucleoproteins were detected in 160 (24...
May 17, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28506635/cryoem-structure-of-an-influenza-virus-receptor-binding-site-antibody-antigen-interface
#4
Yuhang Liu, Junhua Pan, Simon Jenni, Donald D Raymond, Tim Caradonna, Khoi T Do, Aaron G Schmidt, Stephen C Harrison, Nikolaus Grigorieff
Structure-based vaccine design depends on extensive structural analyses of antigen-antibody complexes. Single-particle electron cryomicroscopy (cryoEM) can circumvent some of the problems of x-ray crystallography as a pipeline for obtaining the required structures. We have examined the potential of single-particle cryoEM for determining the structure of influenza-virus hemagglutinin (HA):single-chain Fv (scFv) complexes, by studying a complex we failed to crystallize in pursuing an extended project of the human immune response to influenza vaccines...
May 12, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28505524/inactivated-influenza-virus-vaccines-the-future-of-tiv-and-qiv
#5
REVIEW
Michael Schotsaert, Adolfo García-Sastre
Influenza viruses continue to be a major public health concern, despite the availability of vaccines. Currently licensed influenza vaccines aim at the induction of antibodies that target hemagglutinin, the major antigenic determinant on the surface of influenza virions that is responsible for attachment of the virus to the host cell that is to be infected. Currently licensed influenza vaccines come as inactivated or live attenuated influenza vaccines and are trivalent or quadrivalent as they contain antigens of two influenza A and one or two influenza B strains that circulate in the human population, respectively...
May 12, 2017: Current Opinion in Virology
https://www.readbyqxmd.com/read/28504265/in-vitro-evolution-of-an-influenza-broadly-neutralizing-antibody-is-modulated-by-hemagglutinin-receptor-specificity
#6
Nicholas C Wu, Geramie Grande, Hannah L Turner, Andrew B Ward, Jia Xie, Richard A Lerner, Ian A Wilson
The relatively recent discovery and characterization of human broadly neutralizing antibodies (bnAbs) against influenza virus provide valuable insights into antiviral and vaccine development. However, the factors that influence the evolution of high-affinity bnAbs remain elusive. We therefore explore the functional sequence space of bnAb C05, which targets the receptor-binding site (RBS) of influenza haemagglutinin (HA) via a long CDR H3. We combine saturation mutagenesis with yeast display to enrich for C05 variants of CDR H3 that bind to H1 and H3 HAs...
May 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28502901/neuraminidase-activity-of-blue-eye-disease-porcine-rubulavirus-specificity-affinity-and-inhibition-studies
#7
Gerardo Santos-López, María T Borraz-Argüello, Luis Márquez-Domínguez, Juan Carlos Flores-Alonso, Humberto Ramírez-Mendoza, Bernard Priem, Sébastien Fort, Verónica Vallejo-Ruiz, Julio Reyes-Leyva, Irma Herrera-Camacho
Porcine rubulavirus (PorPV), also known as La Piedad Michoacan Virus (LPMV) causes encephalitis and reproductive failure in newborn and adult pigs, respectively. The hemagglutinin-neuraminidase (HN) glycoprotein is the most exposed and antigenic of the virus proteins. HN plays central roles in PorPV infection; i.e., it recognizes sialic acid-containing cell receptors that mediate virus attachment and penetration; in addition, its neuraminidase (sialic acid releasing) activity has been proposed as a virulence factor...
May 6, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28502308/-preparation-and-identification-of-monoclonal-antibody-against-h1n1-influenza-virus
#8
Yang Liu, Xiangrong Zhao, Chunyan Guo, Yan Li, Guanghua Wang, Daoyan Liang, Pengbo Yu, Yuan Li, Jun Hu
Objective To prepare the monoclonal antibody (mAb) against influenza A virus (H1N1) using purified viral particles as antigen and investigate the characterization of host cells infected with influenza virus utilizing the mAb. Methods A/PR/8 (H1N1) virus was cultured in embryonated chicken eggs and further purified by differential and density gradient centrifugation. The structure of viral particles was identified by transmission electron microcopy (TEM). Immunogenicity of purified virus was evaluated by Balb/c mice immunized with formalin-inactivated virus...
May 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28499086/a-rapid-diagnostic-assay-for-intact-influenza-virus-using-a-high-affinity-hemagglutinin-binding-protein
#9
Caitlin E Anderson, Carly A Holstein, Eva-Maria Strauch, Steven Bennett, Aaron Chevalier, Jorgen W Nelson, Elain Fu, David Baker, Paul Yager
Influenza is a ubiquitous and recurring infection that results in approximately 500,000 deaths globally each year. Commercially available rapid diagnostic tests are based upon detection of the influenza nucleoprotein, which are limited in that they are unable to differentiate by species and require an additional viral lysis step. Sample pre-processing can be minimized or eliminated by targeting the intact influenza virus, thereby reducing assay complexity and leveraging the large number of hemagglutinin proteins on the surface of each virus...
May 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28494003/p2y6-receptors-are-involved-in-mediating-the-effect-of-inactivated-avian-influenza-virus-h5n1-on-il-6-cxcl8-mrna-expression-in-respiratory-epithelium
#10
Nawiya Huipao, Suparerk Borwornpinyo, Suwimon Wiboon-Ut, Craig R Campbell, Il-Ha Lee, Siriphun Hiranyachattada, Chonlaphat Sukasem, Arunee Thitithanyanont, Chumpol Pholpramool, David I Cook, Anuwat Dinudom
One of the key pathophysiologies of H5N1 infection is excessive proinflammatory cytokine response (cytokine storm) characterized by increases in IFN-β, TNF-α, IL-6, CXCL10, CCL4, CCL2 and CCL5 in the respiratory tract. H5N1-induced cytokine release can occur via an infection-independent mechanism, however, detail of the cellular signaling involved is poorly understood. To elucidate this mechanism, the effect of inactivated (β-propiolactone-treated) H5N1 on the cytokine and chemokine mRNA expression in 16HBE14o- human respiratory epithelial cells was investigated...
2017: PloS One
https://www.readbyqxmd.com/read/28493964/non-human-primate-orthologues-of-tmprss2-cleave-and-activate-the-influenza-virus-hemagglutinin
#11
Pawel Zmora, Paulina Molau-Blazejewska, Stephanie Bertram, Kerstin Walendy-Gnirß, Inga Nehlmeier, Anika Hartleib, Anna-Sophie Moldenhauer, Sebastian Konzok, Susann Dehmel, Katherina Sewald, Constantin Brinkmann, Christoph Curths, Sascha Knauf, Jens Gruber, Kerstin Mätz-Rensing, Franziska Dahlmann, Armin Braun, Stefan Pöhlmann
The cellular serine protease TMPRSS2, a member of the type II transmembrane serine protease (TTSP) family, cleaves and activates the hemagglutinin of influenza A viruses (FLUAV) in cell culture and is essential for spread of diverse FLUAV in mice. Non-human primates (NHP), in particular rhesus and cynomolgus macaques, serve as animal models for influenza and experimental FLUAV infection of common marmosets has recently also been reported. However, it is currently unknown whether the NHP orthologues of human TMPRSS2 cleave and activate FLUAV hemagglutinin and contribute to viral spread in respiratory tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28492063/vaccination-with-killed-but-metabolically-active-e-coli-over-expressing-hemagglutinin-elicits-neutralizing-antibodies-to-h1n1-swine-origin-influenza-a-virus
#12
Pei-Feng Liu, Yanhan Wang, Yu-Tsueng Liu, Chun-Ming Huang
There is a need for a fast and simple method for vaccine production to keep up with the pace of a rapidly spreading virus in the early phases of the influenza pandemic. The use of whole viruses produced in chicken eggs or recombinant antigens purified from various expression systems has presented considerable challenges, especially with lengthy processing times. Here, we use the killed but metabolically active (KBMA) Escherichia coli (E. coli) to harbor the hemagglutinin (HA) of swine origin influenza A (H1N1) virus (S-OIV) San Diego/01/09 (SD/H1N1-S-OIV)...
February 2017: Journal of Nature and Science
https://www.readbyqxmd.com/read/28490598/development-of-clade-specific-and-broadly-reactive-live-attenuated-influenza-virus-vaccines-against-rapidly-evolving-h5-subtype-viruses
#13
Kobporn Boonnak, Yumiko Matsuoka, Weijia Wang, Amorsolo L Suguitan, Zhongying Chen, Myeisha Paskel, Mariana Baz, Ian Moore, Hong Jin, Kanta Subbarao
We have developed pandemic live attenuated influenza vaccines (pLAIV) against clade 1 H5N1 viruses on an Ann Arbor cold-adapted (ca) backbone that induced long-term immune memory. In 2015, many human infections caused by a new clade (2.2.1.1) of goose/Guangdong (gs/GD) lineage H5N1 viruses were reported in Egypt that prompted updating of the H5N1 pLAIV. We explored two strategies to generate suitable pLAIVs: first to modify the hemagglutinin gene of a highly pathogenic wild-type (wt) clade 2.2.1.1 virus A/Egypt/N03434/2009 (Egy/09) (H5N1) with its unmodified neuraminidase (NA) gene; this virus was designated Egy/09 ca The second approach was to select a low pathogenicity avian influenza H5 virus that elicited antibodies that cross-reacted with a broad range of H5 viruses, including the Egypt H5N1 viruses and contained a novel NA subtype for humans...
May 10, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28484283/predicting-influenza-antigenicity-from-hemagglutintin-sequence-data-based-on-a-joint-random-forest-method
#14
Yuhua Yao, Xianhong Li, Bo Liao, Li Huang, Pingan He, Fayou Wang, Jiasheng Yang, Hailiang Sun, Yulong Zhao, Jialiang Yang
Timely identification of emerging antigenic variants is critical to influenza vaccine design. The accuracy of a sequence-based antigenic prediction method relies on the choice of amino acids substitution matrices. In this study, we first compared a comprehensive 95 substitution matrices reflecting various amino acids properties in predicting the antigenicity of influenza viruses by a random forest model. We then proposed a novel algorithm called joint random forest regression (JRFR) to jointly consider top substitution matrices...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28475918/inhibition-of-the-infectivity-and-inflammatory-response-of-influenza-virus-by-arbidol-hydrochloride-in-vitro-and-in-vivo-mice-and-ferret
#15
Yutao Wang, Yuewen Ding, Chunguang Yang, Runfeng Li, Qiuling Du, Yanbing Hao, Zhengtu Li, Haiming Jiang, Jin Zhao, Qiaoyan Chen, Zifeng Yang, Zhanlong He
Influenza virus infections are the main contagious respiratory disease with high levels of morbidity and mortality worldwide. Antiviral drugs are indispensable for the prophylaxis and treatment of influenza and other respiratory viral infections. In this study, the Arbidol hydrochloride (ARB), which has been licensed in Russia and China, is used to investigate its anti-viral and anti-inflammatory efficacy in vitro and in vivo. The antiviral results in vitro showed that ARB had a better inhibition on Influenza virus A/PR/8/34 (H1N1), A/Guangdong/GIRD07/09 (H1N1), A/Aichi/2/68 (H3N2), A/HK/Y280/97 (H9N2) with IC50 ranging from 4...
May 2, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28473830/the-length-of-n-glycans-of-recombinant-h5n1-hemagglutinin-influences-the-oligomerization-and-immunogenicity-of-vaccine-antigen
#16
Agnieszka Katarzyna Macioła, Maria Anna Pietrzak, Piotr Kosson, Mariusz Czarnocki-Cieciura, Krzysztof Śmietanka, Zenon Minta, Edyta Kopera
Hemagglutinin glycoprotein (HA) is a principle influenza vaccine antigen. Recombinant HA-based vaccines become a potential alternative for traditional approach. Complexity and variation of HA N-glycosylation are considered as the important factors for the vaccine design. The number and location of glycan moieties in the HA molecule are also crucial. Therefore, we decided to study the effect of N-glycosylation pattern on the H5 antigen structure and its ability to induce immunological response. We also decided to change neither the number nor the position of the HA glycosylation sites but only the glycan length...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28473725/epidemiological-clinical-and-virologic-features-of-two-family-clusters-of-avian-influenza-a-h7n9-virus-infections-in-southeast-china
#17
Jianfeng Xie, Yuwei Weng, Jianming Ou, Lin Zhao, Yanhua Zhang, Jinzhang Wang, Wei Chen, Meng Huang, Wenqiong Xiu, Hongbin Chen, Yongjun Zhang, Binshan Wu, Wenxiang He, Ying Zhu, Libin You, Zhimiao Huang, Canming Zhang, Longtao Hong, Wei Wang, Kuicheng Zheng
This study aimed to investigate the epidemiological, clinical, and virologic characteristics of avian influenza A (H7N9) confirmed cases from two family clusters in Southeast China. Epidemiological data of the H7N9 confirmed cases and their close contacts were obtained through interviews and reviews of medical records. Of the four patients in these two family clusters, two cases had mild symptoms, one had severe symptoms, and one died. Three of the four patients had a history of exposure to live poultry or contaminated environments...
May 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28469192/construction-of-a-highly-efficient-crispr-cas9-mediated-duck-enteritis-virus-based-vaccine-against-h5n1-avian-influenza-virus-and-duck-tembusu-virus-infection
#18
Zhong Zou, Kun Huang, Yanmin Wei, Huanchun Chen, Ziduo Liu, Meilin Jin
Duck enteritis virus (DEV), duck tembusu virus (DTMUV), and highly pathogenic avian influenza virus (HPAIV) H5N1 are the most important viral pathogens in ducks, as they cause significant economic losses in the duck industry. Development of a novel vaccine simultaneously effective against these three viruses is the most economical method for reducing losses. In the present study, by utilizing a clustered regularly interspaced short palindromic repeats (CRISPR)/associated 9 (Cas9)-mediated gene editing strategy, we efficiently generated DEV recombinants (C-KCE-HA/PrM-E) that simultaneously encode the hemagglutinin (HA) gene of HPAIV H5N1 and pre-membrane proteins (PrM), as well as the envelope glycoprotein (E) gene of DTMUV, and its potential as a trivalent vaccine was also evaluated...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28465552/a-reassortant-h9n2-influenza-virus-containing-2009-pandemic-h1n1-internal-protein-genes-acquired-enhanced-pig-to-pig-transmission-after-serial-passages-in-swine
#19
José Carlos Mancera Gracia, Silvie Van den Hoecke, Juergen A Richt, Wenjun Ma, Xavier Saelens, Kristien Van Reeth
Avian H9N2 and 2009 pandemic H1N1 (pH1N1) influenza viruses can infect pigs and humans, raising the concern that H9N2:pH1N1 reassortant viruses could emerge. Such reassortants demonstrated increased replication and transmissibility in pig, but were still inefficient when compared to pH1N1. Here, we evaluated if a reassortant virus containing the hemagglutinin and neuraminidase of A/quail/Hong Kong/G1/1997 (H9N2) in the A/California/04/2009 (pH1N1) backbone could become better adapted to pigs by serial passaging...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28461098/organization-and-dynamics-of-trp14-of-hemagglutinin-fusion-peptide-in-membrane-mimetic-environment
#20
Geetanjali Meher, Hirak Chakraborty
The fusion peptide of influenza hemagglutinin (HA) is important for cellular entry of influenza virus. Previous studies showed that HA fusion peptide assumes a bent structure in membrane environment, which is extremely important for its fusion-promoting activity. In this work, we have measured the organization and dynamics of the tryptophan (Trp14) residue in presence of sodium dodecyl sulfate (SDS) to investigate the conformational flexibility of tryptophan in membrane-mimetic environment. The steady state and time-resolved fluorescence measurements were employed to investigate the location and rotational flexibility of the tryptophan residue in the SDS micelles...
April 28, 2017: Chemistry and Physics of Lipids
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