keyword
MENU ▼
Read by QxMD icon Read
search

Influenza polymerase

keyword
https://www.readbyqxmd.com/read/27926816/identification-of-polymerase-gene-mutations-that-affect-viral-replication-in-h5n1-influenza-viruses-isolated-from-pigeons
#1
Emad Mohamed Elgendy, Yasuha Arai, Norihito Kawashita, Tomo Daidoji, Tatsuya Takagi, Madiha Salah Ibrahim, Takaaki Nakaya, Yohei Watanabe
Highly pathogenic avian influenza virus H5N1 infects a wide range of host species, with a few cases of sporadic pigeon infections reported in the Middle East and Asia. However, the role of pigeons in the ecology and evolution of H5N1 viruses remains unclear. We previously reported two H5N1 virus strains, isolated from naturally infected pigeons in Egypt, that have several unique mutations in their viral polymerase genes. Here, we investigated the effect of these mutations on H5N1 polymerase activity and viral growth and identified three mutations that affected viral polymerase activity...
December 7, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27912070/cardiac-complications-associated-with-the-influenza-viruses-a-subtype-h7n9-or-pandemic-h1n1-in-critically-ill-patients-under-intensive-care
#2
Jiajia Wang, Hua Xu, Xinjing Yang, Daguo Zhao, Shenlang Liu, Xue Sun, Jian-An Huang, Qiang Guo
BACKGROUND AND OBJECTIVE: The clinical presentations and disease courses of patients hospitalized with either influenza A virus subtype H7N9 (H7N9) or 2009 pandemic H1N1 influenza virus (pH1N1) were compared in a recent report, but associated cardiac complications remain unclear. The present retrospective study investigated whether cardiac complications in critically ill patients with H7N9 infections differed from those infected with the pH1N1 strain. METHODS: Suspect cases were confirmed by reverse transcription polymerase chain reaction assays with specific confirmation of the pandemic H1N1 strain at the Centers for Disease Control and Prevention...
November 29, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27904520/a-diagnostic-one-step-real-time-reverse-transcription-polymerase-chain-reaction-method-for-accurate-detection-of-influenza-virus-type-a
#3
Mohammad Amin Behzadi, Mazyar Ziyaeyan, Abdolvahab Alborzi
INTRODUCTION: Influenza A is known as a public health concern worldwide. In this study, a novel one-step real-time reverse transcription polymerase chain reaction (rtRT-PCR) assay was designed and optimized for the detection of influenza A viruses. MATERIAL AND METHODS: The primers and probe were designed based on the analysis of 90 matrix nucleotide sequence data of influenza type A subtypes from the GenBank database of the National Center for Biotechnology Information (NCBI)...
December 1, 2016: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/27902900/sensitivity-and-specificity-estimation-for-the-clinical-diagnosis-of-highly-pathogenic-avian-influenza-in-the-egyptian-participatory-disease-surveillance-program
#4
C Verdugo, I El Masry, Y Makonnen, H Hannah, F Unger, M Soliman, S Galal, J Lubroth, D Grace
Many developing countries lack sufficient resources to conduct animal disease surveillance. In recent years, participatory epidemiology has been used to increase the cover and decrease the costs of surveillance. However, few diagnostic performance assessments have been carried out on participatory methods. The objective of the present study was to estimate the diagnostic performance of practitioners working for the Community-Based Animal Health and Outreach (CAHO) program, which is a participatory disease surveillance system for the detection of highly pathogenic avian influenza outbreaks in Egypt...
December 2016: Avian Diseases
https://www.readbyqxmd.com/read/27889648/pb2-substitutions-v598t-i-increase-the-virulence-of-h7n9-influenza-a-virus-in-mammals
#5
Meng Hu, Shuofeng Yuan, Ke Zhang, Kailash Singh, Qiang Ma, Jie Zhou, Hin Chu, Bo-Jian Zheng
PB2 is one of the subunits of the influenza A virus (IAV) polymerase complex. By bioinformatics analysis we identified PB2 substitutions at positions 389 and 598 among IAV isolates from humans, which might associate with viral pathogenicity. To evaluate the biological significance of these substitutions, PB2-K389R and -V598T/I mutant viruses of avian H7N9 IAVs were generated by reverse genetics. Compared to the wild type, the mutant viruses displayed an enhanced growth capacity in human and mammalian cells...
November 24, 2016: Virology
https://www.readbyqxmd.com/read/27886255/amino-acid-substitutions-v63i-or-a37s-i61t-v63i-v100a-in-the-pa-n-terminal-domain-increase-the-virulence-of-h7n7-influenza-a-virus
#6
Meng Hu, Hin Chu, Ke Zhang, Kailash Singh, Cun Li, Shuofeng Yuan, Billy K C Chow, Wenjun Song, Jie Zhou, Bo-Jian Zheng
The PA N-terminal domain (PA-Nter) is essential for viral transcription and replication. Here we identified PA-Nter substitutions A37S, I61T, V63I and V100A in recently emerged avian influenza A viruses (IAVs) with potential effect on virus pathogenicity and/or host adaptation. We introduced the identified PA-Nter substitutions into avian H7N7 IAV by reverse genetics. Our results showed that single substitution V63I and combined substitutions, I61T/V63I and A37S/I61T/V63I/V100A (Mfour), significantly increased virus growth capacity in mammalian cells...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27872775/infrarenal-aorta-thrombosis-associated-with-h1n1-influenza-a-virus-infection
#7
Can Hüzmeli, Mustafa Saglam, Ali Arıkan, Barıs Doner, Gulay Akıncı, Ferhan Candan
Influenza viruses are members of the Orthomyxoviridae family, of which influenza A, B, and C viruses constitute three separate genera. Arterial thrombosis associated with H1N1 influenza A virus infection has rarely been reported. A Turkish man aged 28 years was admitted to our emergency department with dyspnea, bilateral lower extremity insensitivity, and cold. He reported symptoms of fever, myalgia, and cough, which he had had for fifteen days before being admitted to our hospital. The patient was tested for pandemic influenza A (H1N1) virus using polymerase chain reaction (PCR) tests, which were positive...
2016: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/27862840/differential-nucleocytoplasmic-shuttling-of-the-nucleoprotein-of-influenza-a-viruses-and-association-with-host-tropism
#8
Jing Li, Weinan Zheng, Lidan Hou, Can Chen, Wenhui Fan, Hongren Qu, Jingwen Jiang, Jinhua Liu, George F Gao, Jiyong Zhou, Lei Sun, Wenjun Liu
The nucleoprotein (NP) of influenza A virus plays a crucial role in virus replication, infectivity, and host adaptation. As a major component of the viral ribonucleoprotein complexes (vRNP), NP initiates vRNP shuttling between the nucleus and cytoplasm in the host cell. However, the characteristics of the nucleocytoplasmic shuttling of NP from H1N1 influenza A virus still remain unclear. In the present study, the subcellular localization and the related key residues of the H1N1 influenza virus NP were identified and evaluated...
November 8, 2016: Cellular Microbiology
https://www.readbyqxmd.com/read/27859043/monocyte-derived-dendritic-cells-enhance-protection-against-secondary-influenza-challenge-by-controlling-the-switch-in-cd82-t-cell-immunodominance
#9
Jazmina L G Cruz, José V Pérez-Girón, Anja Lüdtke, Sergio Gómez-Medina, Paula Ruibal, Juliana Idoyaga, César Muñoz-Fontela
Influenza virus infection triggers an increase in the number of monocyte-derived dendritic cells (moDCs) in the respiratory tract, but the role of these cells during antiviral immunity is still unclear. Here we show that during influenza infection, moDCs dominate the late activation of CD8(+) T cells and trigger the switch in immunodominance of the CD8(+) T-cell response from acidic polymerase (PA)-specificity to nucleoprotein (NP)-specificity. Abrogation of monocyte recruitment or depletion of moDCs strongly compromised host resistance to secondary influenza challenge...
November 18, 2016: European Journal of Immunology
https://www.readbyqxmd.com/read/27852861/pre-mrna-processing-factor-prp18-is-a-stimulatory-factor-of-influenza-virus-rna-synthesis-and-possesses-the-np-chaperone-activity
#10
M Minakuchi, K Sugiyama, Y Kato, T Naito, M Okuwaki, A Kawaguchi, K Nagata
: The genome of influenza virus (vRNA) is associated with nucleoprotein (NP) and viral RNA-dependent RNA polymerases, and forms helical viral ribonucleoprotein (vRNP) complexes. NP-vRNA complex is the biologically active template for RNA synthesis by the viral polymerase. Previously, we identified human pre-mRNA processing factor 18 (Prp18) as a stimulatory factor for viral RNA synthesis using an yeast replicon system and single-gene deletion library of yeast (Naito T, Kiyasu Y, Sugiyama K, Kimura A, Nakano R, Matsukage A, Nagata K...
November 16, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27849220/surveillance-of-antiviral-resistance-markers-in-argentina-detection-of-e119v-neuraminidase-mutation-in-a-post-treatment-immunocompromised-patient
#11
Andrea Pontoriero, Martín Avaro, Estefania Benedetti, Mara Russo, Andrea Czech, Natalia Periolo, Ana Campos, Ana Zamora, Elsa Baumeister
Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase (NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir administration is E119V mutation. We describe the emergence of a mixed viral population with the E119E/V mutation in the NA protein sequence in a post-treatment influenza sample collected from an immunocompromised patient in Argentina...
November 16, 2016: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/27845268/evidence-for-influenza-b-virus-lineage-shifts-and-reassortants-circulating-in-thailand-in-2014-2016
#12
Nipaporn Tewawong, Nungruthai Suntronwong, Sumeth Korkong, Apiradee Theamboonlers, Sompong Vongpunsawad, Yong Poovorawan
Towards the surveillance of seasonal influenza viruses between August 2015 and June 2016, respiratory samples (n=3390) were collected from Thai patients with influenza-like illness. One-hundred fifty-seven (4.6%) samples tested positive for influenza B virus by real-time reverse-transcription polymerase chain reaction (RT-PCR). While the influenza B virus Yamagata lineage strains were more prevalent than the Victoria lineage strains in 2015 (77.5% vs. 22.5%), the Victoria lineage strains appeared to dominate the first half of 2016 (62...
November 12, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27836481/a-systematic-review-of-rapid-diagnostic-tests-for-influenza-considerations-for-the-community-pharmacist
#13
REVIEW
Renee R Koski, Michael E Klepser
OBJECTIVES: Rapid influenza diagnostic tests (RIDTs) have a potential role in community pharmacy to optimize influenza infection management. The U.S. Food and Drug Administration (FDA) proposed changes to the classification of RIDTs may affect their use in community pharmacy. We reviewed the performance and features of RIDTs likely to meet FDA-proposed reclassification requirements. DATA SOURCES: PubMed and Medline database searches were performed using the terms "Sofia Influenza A and B Fluorescent Immunoassay," "BD Veritor System for Rapid Detection of Flu A and B", and "Alere i Influenza A and B...
November 8, 2016: Journal of the American Pharmacists Association: JAPhA
https://www.readbyqxmd.com/read/27834632/viral-factors-in-influenza-pandemic-risk-assessment
#14
Marc Lipsitch, Wendy Barclay, Rahul Raman, Charles J Russell, Jessica A Belser, Sarah Cobey, Peter M Kasson, James O Lloyd-Smith, Sebastian Maurer-Stroh, Steven Riley, Catherine Aa Beauchemin, Trevor Bedford, Thomas C Friedrich, Andreas Handel, Sander Herfst, Pablo R Murcia, Benjamin Roche, Claus O Wilke, Colin A Russell
The threat of an influenza A virus pandemic stems from continual virus spillovers from reservoir species, a tiny fraction of which spark sustained transmission in humans. To date, no pandemic emergence of a new influenza strain has been preceded by detection of a closely related precursor in an animal or human. Nonetheless, influenza surveillance efforts are expanding, prompting a need for tools to assess the pandemic risk posed by a detected virus. The goal would be to use genetic sequence and/or biological assays of viral traits to identify those non-human influenza viruses with the greatest risk of evolving into pandemic threats, and/or to understand drivers of such evolution, to prioritize pandemic prevention or response measures...
November 11, 2016: ELife
https://www.readbyqxmd.com/read/27832151/nationwide-trends-in-bacterial-meningitis-before-the-introduction-of-13-valent-pneumococcal-conjugate-vaccine-burkina-faso-2011-2013
#15
Dinanibè Kambiré, Heidi M Soeters, Rasmata Ouédraogo-Traoré, Isaïe Medah, Lassana Sangare, Issaka Yaméogo, Guetawendé Sawadogo, Abdoul-Salam Ouédraogo, Soumeya Hema-Ouangraoua, Lesley McGee, Velusamy Srinivasan, Flavien Aké, Malika Congo-Ouédraogo, Soufian Sanou, Absatou Ky Ba, Ryan T Novak, Chris Van Beneden
BACKGROUND: Following introduction of Haemophilus influenzae type b vaccine in 2006 and serogroup A meningococcal conjugate vaccine in 2010, Streptococcus pneumoniae (Sp) became the leading cause of bacterial meningitis in Burkina Faso. We describe bacterial meningitis epidemiology, focusing on pneumococcal meningitis, before 13-valent pneumococcal conjugate vaccine (PCV13) introduction in the pediatric routine immunization program in October 2013. METHODS: Nationwide population-based meningitis surveillance collects case-level demographic and clinical information and cerebrospinal fluid (CSF) laboratory results...
2016: PloS One
https://www.readbyqxmd.com/read/27831626/-non-fatal-case-of-h1n1-influenza-encephalopathy-in-a-pediatric-patient
#16
María Del Carmen Valdivia-Tapia, Nilton Yhuri Carreazo
A 2-year-old girl presented with fever, catarrhal symptoms, and focal right hemispheric seizures that persisted and led to signs of intracranial hypertension. An influenza A H1N1 infection was confirmed via polymerase chain reaction analysis of a nasopharyngeal swab. The patient, who was not treated with oseltamavir, has responded favorably to supportive measures.
July 2016: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/27807150/comparison-between-saliva-and-nasopharyngeal-swab-specimens-for-the-detection-of-respiratory-viruses-by-multiplex-reverse-transcription-polymerase-chain-reaction
#17
Young-Gon Kim, Seung Gyu Yun, Min Young Kim, Kwisung Park, Chi Hyun Cho, Soo Young Yoon, Myung Hyun Nam, Chang Kyu Lee, Yun Jung Cho, Chae Seung Lim
Nasopharyngeal swab (NPS) is being widely used as a specimen for multiplex real-time reverse transcription polymerase chain reaction (RT-PCR) for respiratory virus detection. However, it remains unclear whether NPS is optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS causes coughing in most patients, which possibly increases the risk of nosocomial spread. In this study, paired NPS and saliva specimens were collected from 236 adult male patients with suspected acute respiratory illnesses...
November 2, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27803181/annotating-protein-functional-residues-by-coupling-high-throughput-fitness-profile-and-homologous-structure-analysis
#18
Yushen Du, Nicholas C Wu, Lin Jiang, Tianhao Zhang, Danyang Gong, Sara Shu, Ting-Ting Wu, Ren Sun
: Identification and annotation of functional residues are fundamental questions in protein sequence analysis. Sequence and structure conservation provides valuable information to tackle these questions. It is, however, limited by the incomplete sampling of sequence space in natural evolution. Moreover, proteins often have multiple functions, with overlapping sequences that present challenges to accurate annotation of the exact functions of individual residues by conservation-based methods...
November 1, 2016: MBio
https://www.readbyqxmd.com/read/27798334/serum-25-hydroxyvitamin-d-was-not-associated-with-influenza-virus-infection-in-children-and-adults-in-hong-kong-2009-2010
#19
Cuiling Xu, Vicky J Fang, Ranawaka Apm Perera, Andrea May-Sin Kam, Sophia Ng, Yap-Hang Chan, Kwok-Hung Chan, Dennis Km Ip, Js Malik Peiris, Benjamin J Cowling
BACKGROUND: Some studies have hypothesized that vitamin D may have a role to play in protection against influenza virus infections and illnesses, and that seasonal fluctuation in serum 25-hydroxyvitamin D [25(OH)D] may affect seasonal patterns of influenza virus infections. OBJECTIVE: We aimed to investigate whether serum 25(OH)D concentrations were associated with the incidence of influenza virus infections and illnesses in children and adults in Hong Kong. METHODS: In 2009-2010, 3030 children and adults of all ages from 796 households in Hong Kong were followed up to identify acute respiratory illnesses...
October 26, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27793117/predictors-of-influenza-among-older-adults-in-the-emergency-department
#20
Po-Po Lam, Brenda L Coleman, Karen Green, Jeff Powis, David Richardson, Kevin Katz, Bjug Borgundvaag, Telisha Smith-Gorvie, Jeffrey C Kwong, Susan J Bondy, Allison McGeer
BACKGROUND: Diagnosis of influenza in older adults may be complicated by atypical presentations or when patients present with complications of an underlying illness. We aimed to identify clinical characteristics and epidemiological factors associated with influenza among community-dwelling adults aged ≥60 years presenting to emergency departments. METHODS: We identified patients with influenza-compatible chief complaints presenting to emergency departments of six acute care hospitals in Ontario, Canada during the 2011/12 and 2012/13 influenza seasons...
October 28, 2016: BMC Infectious Diseases
keyword
keyword
61704
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"