keyword
MENU ▼
Read by QxMD icon Read
search

DNA dipstick

keyword
https://www.readbyqxmd.com/read/27819063/multiplex-flow-assays
#1
Kristofer J Haushalter, Srinivas Vetcha, Robert C Haushalter
Lateral flow or dipstick assays (e.g., home pregnancy tests), where an analyte solution is drawn through a porous membrane and is detected by localization onto a capture probe residing at a specific site on the flow strip, are the most commonly and extensively used type of diagnostic assay. However, after over 30 years of use, these assays are constrained to measuring one or a few analytes at a time. Here, we describe a completely general method, in which any single-plex lateral flow assay is transformed into a multiplex assay capable of measuring an arbitrarily large number of analytes simultaneously...
October 31, 2016: ACS Omega
https://www.readbyqxmd.com/read/27816495/simple-detection-of-single-nucleotide-polymorphism-in-plasmodium-falciparum-by-snp-lamp-assay-combined-with-lateral-flow-dipstick
#2
Suganya Yongkiettrakul, Jantana Kampeera, Wanwisa Chareanchim, Roonglawan Rattanajak, Wichai Pornthanakasem, Wansika Kiatpathomchai, Darin Kongkasuriyachai
The significant strides made in reducing global malaria burden over the past decades are being threatened by the emergence of multi-drug resistant malaria. Mechanisms of resistance to several classes of antimalarial drugs have been linked to key mutations in the Plasmodium falciparum genes. Pyrimethamine targets the dihydrofolate reductase of the bifunctional dihydrofolate reductase thymidylate synthase (DHFR-TS), and specific point mutations in the dhfr-ts gene have been assigned to resistant phenotypes. Several molecular methods are available to detect the mutant genotypes including DNA sequencing and PCR-based methods...
November 2, 2016: Parasitology International
https://www.readbyqxmd.com/read/27618437/mechanochemical-sensing-of-single-and-few-hg-ii-ions-using-polyvalent-principles
#3
Shankar Mandal, Sangeetha Selvam, Prakash Shrestha, Hanbin Mao
Sensitivity of biosensors is set by the dissociation constant (Kd) between analytes and probes. Although potent amplification steps can be accommodated between analyte recognition and signal transduction in a sensor to improve the sensitivity 4-6 orders of magnitude below Kd, they compromise temporal resolution. Here, we demonstrated mechanochemical sensing that broke the Kd limit by 9 orders of magnitude for Hg detection without amplifications. Analogous to trawl fishing, we introduced multiple Hg binding units (thymine (T)-T pairs) in a molecular trawl made of two poly-T strands...
October 4, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27577576/recombinase-polymerase-amplification-combined-with-a-lateral-flow-dipstick-for-rapid-and-visual-detection-of-schistosoma-japonicum
#4
Kui Sun, Weiwei Xing, Xinling Yu, Wenliang Fu, Yuanyuan Wang, Minji Zou, Zhihong Luo, Donggang Xu
BACKGROUND: With the continuous decline in prevalence and intensity of Schistosoma japonicum infection in China, more accurate and sensitive methods suitable for field detection become much needed for schistosomiasis control. Here, a novel rapid and visual detection method based on the combination of recombinase polymerase amplification (RPA) and lateral flow dipstick (LFD) was developed to detect S. japonicum DNA in fecal samples. RESULTS: The LFD-RPA assay targeting SjR2 could detect 5 fg S...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/26991017/hemoculture-and-direct-sputum-detection-of-meca-mediated-methicillin-resistant-staphylococcus-aureus-by-loop-mediated-isothermal-amplification-in-combination-with-a-lateral-flow-dipstick
#5
Kawin Nawattanapaiboon, Photchanathorn Prombun, Pitak Santanirand, Apirom Vongsakulyanon, Toemsak Srikhirin, Boonsong Sutapun, Wansika Kiatpathomchai
This study reports loop-mediated isothermal amplification (LAMP) for rapid detection of methicillin-resistant Staphylococcus aureus from direct clinical specimens. Four primers including outer and inner primers were specifically designed on the two target sequences-femB to identify S. aureus and mecA to identify antibiotic-resistant gene. Reference strains including various species of gram-positive/gram-negative isolates were used to evaluate and optimize LAMP assays. The optimum LAMP condition was found at 63°C within 70 min assay time (include hybridization with FITC probe for 5 min and further 5 min for reading the results on the lateral flow dipstick)...
September 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/26921043/rapid-and-sensitive-detection-of-babesia-bovis-and-babesia-bigemina-by-loop-mediated-isothermal-amplification-combined-with-a-lateral-flow-dipstick
#6
Yimin Yang, Qun Li, Suhua Wang, Xueqiu Chen, Aifang Du
Babesia spp. are apicomplexan protozoan parasites of the red blood cells of mammals and are transmitted by ticks. Bovine babesiosis mainly caused by Babesia bovis and Babesia bigemina occurs worldwide, which is a great threat to animal health. Microscopy examination is a gold standard for the diagnosis of babesiosis. However, its sensitivity is too low. This study was conducted to establish a simple, efficient and fast LAMP-LFP method used for early diagnosis of animal babesiosis. LAMP was developed with a set of four primers targeting and amplifying six distinct regions of cytochrome b gene of Babesia spp...
March 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/26912138/pcr-dipstick-dna-chromatography-for-profiling-of-a-subgroup-of-caries-associated-bacterial-species-in-plaque-from-healthy-coronal-surfaces-and-periodontal-pockets
#7
Lingyang Tian, Takuichi Sato, Kousuke Niwa, Mitsuo Kawase, Gen Mayanagi, Jumpei Washio, Nobuhiro Takahashi
The onset of plaque-mediated disease, including dental caries and periodontal diseases, is highly associated with compositional change of the resident microflora from the ecological perspective. As specific bacterial profiles have been linked to different disease stages, microbial compositional measurements might therefore have great value for clinical diagnosis. Previously we have reported a dry-reagent strip biosensor-PCR-dipstick DNA chromatography, which utilized molecular recognition of oligonucleotides and biotin-streptavidin, and the optical property of colored microspheres, for semiquantifying a five-membered subgroup of caries-associated bacterial species in supragingival plaque from healthy coronal surfaces of teeth...
2016: Biomedical Research
https://www.readbyqxmd.com/read/26850038/mutations-of-pvdhfr-and-pvdhps-genes-in-vivax-endemic-malaria-areas-in-kota-marudu-and-kalabakan-sabah
#8
Umi Rubiah Sastu, Noor Rain Abdullah, Nor Azrina Norahmad, Muhammad Nor Farhan Saat, Prem Kumar Muniandy, Jenarun Jelip, Moizin Tikuson, Norsalleh Yusof, Hasidah Mohd Sidek
BACKGROUND: Malaria cases persist in some remote areas in Sabah and Sarawak despite the ongoing and largely successful malaria control programme conducted by the Vector Borne Disease Control Programme, Ministry Of Health, Malaysia. Point mutations in the genes that encode the two enzymes involved in the folate biosynthesis pathway, dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) enzymes confer resistance to pyrimethamine and sulfadoxine respectively, in both Plasmodium falciparum and P...
2016: Malaria Journal
https://www.readbyqxmd.com/read/26781105/%C3%AE-wo-panel-molecular-testing-for-genetic-predisposition-for-thrombosis-using-multi-allele-visual-biosensors
#9
Nikolaos Fountoglou, Margarita Petropoulou, Alexandra Iliadi, Theodore K Christopoulos, Penelope C Ioannou
Thrombosis is considered as the most typical example of multigenic/multifactorial disorder. The three most common genetic risk factors for thrombotic disorders are the G1691A mutation in factor V gene (FV Leiden), the G20210Α mutation in prothrombin gene (FII), and the C677T mutation in the methylenetetrahydrofolate reductase (MTHFR) gene. An additional panel of biomarkers predisposing for thrombotic events includes the H1299R variant in factor V gene (HR2), A1298C variant in MTHFR gene, the V34L mutation in fibrinogen stabilizing factor XIII (FXIII) gene as well as the 4G/5G polymorphism in plasminogen activator inhibitor type-1 (PAI-1) gene...
March 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/26709307/development-of-multiplex-loop-mediated-isothermal-amplification-m-lamp-label-based-gold-nanoparticles-lateral-flow-dipstick-biosensor-for-detection-of-pathogenic-leptospira
#10
A B Nurul Najian, E A R Engku Nur Syafirah, Nabilah Ismail, Maizan Mohamed, Chan Yean Yean
In recent years extensive numbers of molecular diagnostic methods have been developed to meet the need of point-of-care devices. Efforts have been made towards producing rapid, simple and inexpensive DNA tests, especially in the diagnostics field. We report on the development of a label-based lateral flow dipstick for the rapid and simple detection of multiplex loop-mediated isothermal amplification (m-LAMP) amplicons. A label-based m-LAMP lateral flow dipstick assay was developed for the simultaneous detection of target DNA template and a LAMP internal control...
January 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/26625736/techniques-for-evaluation-of-lamp-amplicons-and-their-applications-in-molecular-biology
#11
REVIEW
Mohammad Javad Dehghan Esmatabadi, Ali Bozorgmehr, Hesam Motalebzadeh, Narges Bodaghabadi, Baharak Farhangi, Sadegh Babashah, Majid Sadeghizadeh
Loop-mediated isothermal amplification (LAMP) developed by Notomi et al. (2000) has made it possible to amplify DNA with high specificity, efficiency and rapidity under isothermal conditions. The ultimate products of LAMP are stem-loop structures with several inverted repeats of the target sequence and cauliflower-like patterns with multiple loops shaped by annealing between every other inverted repeats of the amplified target in the similar strand. Because the amplification process in LAMP is achieved by using four to six distinct primers, it is expected to amplify the target region with high selectivity...
2015: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/26611141/a-paper-and-plastic-device-for-the-combined-isothermal-amplification-and-lateral-flow-detection-of-plasmodium-dna
#12
Michael S Cordray, Rebecca R Richards-Kortum
BACKGROUND: Isothermal amplification techniques are emerging as a promising method for malaria diagnosis since they are capable of detecting extremely low concentrations of parasite target while mitigating the need for infrastructure and training required by other nucleic acid based tests. Recombinase polymerase amplification (RPA) is promising for further development since it operates in a short time frame (<30 min) and produces a product that can be visually detected on a lateral flow dipstick...
2015: Malaria Journal
https://www.readbyqxmd.com/read/26542604/alcohol-dehydrogenase-1b-rs1229984-and-aldehyde-dehydrogenase-2-rs671-genotypes-and-alcoholic-ketosis-are-associated-with-the-serum-uric-acid-level-in-japanese-alcoholic-men
#13
Akira Yokoyama, Tetsuji Yokoyama, Takeshi Mizukami, Toshifumi Matsui, Mitsuru Kimura, Sachio Matsushita, Susumu Higuchi, Katsuya Maruyama
AIMS: To identify determinants of hyperuricemia in alcoholics. METHODS: The serum uric acid (UA) levels of 1759 Japanese alcoholic men (≥40 years) were measured on their first visit or within 3 days after admission; ADH1B and ALDH2 genotyping on blood DNA samples were performed. Dipstick urinalyses for ketonuria and serum UA measurements were simultaneously performed for 621 men on their first visit. RESULTS: Serum UA levels of >416 μmol/l (7...
May 2016: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/26365226/rapid-detection-of-bombyx-mori-nucleopolyhedrovirus-bmnpv-by-loop-mediated-isothermal-amplification-assay-combined-with-a-lateral-flow-dipstick-method
#14
Yang Zhou, Jiege Wu, Feng Lin, Naifu Chen, Shaofei Yuan, Lina Ding, Li Gao, Bangxing Hang
The Bombyx mori nucleopolyhedrovirus (BmNPV) is a principal pathogen of the domestic silkworm. The disease often breaks out in sericultural countries and due to its high infectivity; it is difficult to control, resulting in heavy economic loss. In order to develop a rapid, sensitive visual detection and simple-to-use novel technology for detection of BmNPV, a loop-mediated isothermal amplification (LAMP) assay combined with a lateral flow dipstick (LFD) method was described. In this study, a set of four primers and a labeled probe were designed specifically to recognize six distinct regions of the BmNPV gp41 gene, and the LAMP for the detection of BmNPV was developed by isothermal amplification at 61 °C for 45 min, followed by hybridization with an FITC-labeled DNA probe for 5 min and detected by LFD within 5 min...
December 2015: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/26055744/visceral-leishmaniasis-caused-by-leishmania-infantum-in-salta-argentina-possible-reservoirs-and-vectors
#15
Paola A Barroso, Jorge D Marco, Fabricio M Locatelli, Rubén M Cardozo, Carlos L Hoyos, María C Mora, María F García Bustos, Inés López-Quiroga, Tatsuyuki Mimori, Alberto G Gentile, Alejandra B Barrio, Masataka Korenaga, Yoshihisha Hashiguchi, Miguel A Basombrío
Cases of human visceral leishmaniasis (HVL) were not recorded until recently in the Chaco region of northwestern Argentina. Dogs were surveyed at the sites of infection of two HVL index cases in the Chaco region of Salta province. Canine cases (CanL) were diagnosed by two parasitological methods, two molecular methods targeting mini- and maxicircle DNA, and immunochromatographic dipstick. Among 77 dogs studied, 10 (13%) were found infected with Leishmania spp. In seven dogs and two humans, the infecting species was typed as Leishmania (Leishmania) infantum...
August 2015: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/25941520/ff-nano-short-functionalized-nanorods-derived-from-ff-f1-fd-or-m13-filamentous-bacteriophage
#16
Sadia Sattar, Nicholas J Bennett, Wesley X Wen, Jenness M Guthrie, Len F Blackwell, James F Conway, Jasna Rakonjac
F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genetically modified viruses. We have now developed a system for production and purification of very short functionalized Ff-phage-derived nanorods, named Ff-nano, that are only 50 nm in length...
2015: Frontiers in Microbiology
https://www.readbyqxmd.com/read/25919051/rapid-authentication-of-cordyceps-by-lateral-flow-dipstick
#17
Yuk-Lau Wong, Ka-Lok Wong, Pang-Chui Shaw
Cordyceps (Dongchongxiacao), a valuable traditional Chinese medicine, is composed of the fruiting body of Ophiocordyceps sinensis (Family: Ophiocordycipitaceae) on a caterpillar of ghost-moth species (Family: Hepialidae). Owing to its multiple potential functions, Cordyceps are in great demand and represent significant economic value. Adulterants or substitutes named Cordyceps or Chongcao from related fungi have been reported. In this study, polymerase chain reaction (PCR) coupled with a lateral flow dipstick (LFD) system was developed to distinguish genuine herb O...
2015: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/25892676/multi-allele-dna-biosensor-for-the-rapid-genotyping-of-nondeletion-alpha-thalassaemia-mutations-in-hba1-and-hba2-genes-by-means-of-multiplex-primer-extension-reaction
#18
Margarita Petropoulou, Amalia Poula, Jan Traeger-Synodinos, Emmanuel Kanavakis, Theodore K Christopoulos, Penelope C Ioannou
BACKGROUND: Alpha-thalassaemia is an autosomal recessive disorder characterized by defective production of the alpha chain of haemoglobin. It is caused mainly by deletions of one or both of the duplicated alpha-globin genes on chromosome 16, and/or by nucleotide variations, known as "nondeletion" mutations. Definition of the alpha globin genotype in carriers supports genetic counselling, and in patients with Hb H disease is useful to predict prognosis and management options. Here, we report a method that facilitates direct detection by naked eye of the 13 most common "nondeletion" alpha-globin gene mutations in populations around the Mediterranean and Middle East...
June 15, 2015: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/25732426/lateral-flow-devices-for-nucleic-acid-analysis-exploiting-quantum-dots-as-reporters
#19
Eleni A Sapountzi, Sotirios S Tragoulias, Despina P Kalogianni, Penelope C Ioannou, Theodore K Christopoulos
There is a growing interest in the development of biosensors in the form of simple lateral flow devices that enable visual detection of nucleic acid sequences while eliminating several steps required for pipetting, incubation and washing out the excess of reactants. In this work, we present the first dipstick-type nucleic acid biosensors based on quantum dots (QDs) as reporters. The biosensors enable sequence confirmation of the target DNA by hybridization and simple visual detection of the emitted fluorescence under a UV lamp...
March 15, 2015: Analytica Chimica Acta
https://www.readbyqxmd.com/read/25562958/-rapid-detection-of-macrobrachium-rosenbergii-nodavirus-isolated-in-china-by-a-reverse-transcription-loop-mediated-isothermal-amplification-assay-combined-with-a-lateral-flow-dipstick-method
#20
Feng Lin, Li Liu, Gui-Jie Hao, Zheng Cao, Peng-Cheng Sheng, Ying-Lei Wu, Jin-Yu Shen
White coloration of the muscle of the giant river prawn (Macrobrachium rosenbergii) is a serious problem in China. The Macrobrachium rosenbergii Nodavirus (MrNV) has been confirmed to be the pathogen that causes this disorder. To develop a rapid, sensitive and specific technology for the detection of Macrobrachium rosenbergii Nodavirus isolated from China (MrNV-China), a reverse-transcription loop- mediated isothermal amplification assay combined with a lateral flow dipstick (RT-LAMP-LFD) assay method is described...
September 2014: Bing du Xue Bao, Chinese Journal of Virology
keyword
keyword
61584
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"