keyword
MENU ▼
Read by QxMD icon Read
search

Type III Secretion System

keyword
https://www.readbyqxmd.com/read/28642776/regulation-involved-in-colonization-of-intercellular-spaces-of-host-plants-in-ralstonia-solanacearum
#1
REVIEW
Yasufumi Hikichi, Yuka Mori, Shiho Ishikawa, Kazusa Hayashi, Kouhei Ohnishi, Akinori Kiba, Kenji Kai
A soil-borne bacterium Ralstonia solanacearum invading plant roots first colonizes the intercellular spaces of the root, and eventually enters xylem vessels, where it replicates at high levels leading to wilting symptoms. After invasion into intercellular spaces, R. solanacearum strain OE1-1 attaches to host cells and expression of the hrp genes encoding components of the type III secretion system (T3SS). OE1-1 then constructs T3SS and secrets effectors into host cells, inducing expression of the host gene encoding phosphatidic acid phosphatase...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28641564/investigation-on-the-interaction-of-pseudomonas-syringae-effector-avrpto-with-atrabe1d-gtpase
#2
Xiaomin Hou, Yi Gao
The tomato bacterial speck is a worldwide disease. It is caused by the infection of pathogenic pseudomonas syringae pv. tomato which delivers the effector AvrPto into the host cells via the type III secretion system. AvrPto interacts Rab8 subfamily protein in the GTP-bound form and participates in the response to pathogen infection, but the pathogenic mechanism involved by Rab8 subfamily remains elusive. Here, we investigated on the interrelationship of AvrPto8-159 with Arabidopsis thaliana GTP-restricted Rab8 subfamily protein AtRabE1d13-185Q74L through some biophysical and biochemical methods...
June 21, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28640910/correction-btea-secreted-from-the-bordetella-bronchiseptica-type-iii-secetion-system-induces-necrosis-through-an-actin-cytoskeleton-signaling-pathway-and-inhibits-phagocytosis-by-macrophages
#3
Asaomi Kuwae, Fumitaka Momose, Kanna Nagamatsu, Yasuharu Suyama, Akio Abe
[This corrects the article DOI: 10.1371/journal.pone.0148387.].
2017: PloS One
https://www.readbyqxmd.com/read/28640881/t3ss-effector-vopl-inhibits-the-host-ros-response-promoting-the-intracellular-survival-of-vibrio-parahaemolyticus
#4
Marcela de Souza Santos, Dor Salomon, Kim Orth
The production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (NADPH) oxidase complex is an important mechanism for control of invading pathogens. Herein, we show that the gastrointestinal pathogen Vibrio parahaemolyticus counteracts reactive oxygen species (ROS) production using the Type III Secretion System 2 (T3SS2) effector VopL. In the absence of VopL, intracellular V. parahaemolyticus undergoes ROS-dependent filamentation, with concurrent limited growth. During infection, VopL assembles actin into non-functional filaments resulting in a dysfunctional actin cytoskeleton that can no longer mediate the assembly of the NADPH oxidase at the cell membrane, thereby limiting ROS production...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28630074/the-enterohaemorrhagic-escherichia-coli-effector-espw-triggers-actin-remodeling-in-a-rac1-dependent-manner
#5
Pamela Sandu, Valerie F Crepin, Hauke Drechsler, Andrew D McAinsh, Gad Frankel, Cedric N Berger
Enterohaemorrhagic Escherichia coli (EHEC) is a diarrheagenic pathogen that colonizes the gut mucosa and induces attaching-and-effacing lesions. EHEC employs a type III secretion system (T3SS) to translocate 50 effector proteins that hijack and manipulate host cell signalling pathways, which allow bacterial colonization, subversion of immune responses and disease progression. The aim of this study was to characterize the T3SS effector EspW. We found espW in the sequenced O157:H7 and non-O157 EHEC strains as well as in Shigella boydii Furthermore, a truncated version of EspW, containing the first 206 residues is present in EPEC strains belonging to serotype O55:H7...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28630070/regulation-of-type-iii-secretion-of-translocon-and-effector-proteins-by-the-esab-esal-esam-complex-in-edwardsiella-tarda
#6
Lu Yi Liu, Pin Nie, Hong Bing Yu, Hai Xia Xie
The type III secretion system (T3SS) plays a crucial role in the pathogenesis of many Gram-negative bacteria, including Edwardsiella tarda, an important fish pathogen. Within the E. tarda T3SS, there are three proteins (EsaB/EsaL/EsaM) that are homologous to proteins present in many other bacteria, including SpiC/SsaL/SsaM in Salmonella, SepD/SepL/CesL in EPEC and EHEC, and YscB/YopN/SycN in Yersinia EsaL was found to interact with both EsaB and EsaM within the bacterial cell, as revealed by a co-immunoprecipitation assay...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28627966/further-evidence-of-cucurbit-host-specificity-among-acidovorax-citrulli-groups-based-on-a-detached-melon-fruit-pathogenicity-assay
#7
Lichun Yan, Hu Baishi, Gong Chen, Mei Zhao, Ron Ricardo Walcott
Bacterial fruit blotch (BFB), caused by the gram-negative bacterium Acidovorax citrulli, is a serious economic threat to cucurbit crop production worldwide. A. citrulli strains can be divided into two genetically distinct groups with group I strains infecting a range of cucurbit species, and group II strains being predominantly associated with watermelon. Group I and II A. citrulli strains differ in their arsenal of type III secreted (T3S) effector proteins and we hypothesize that these effectors are critical for cucurbit host preference...
June 19, 2017: Phytopathology
https://www.readbyqxmd.com/read/28627613/translocation-of-enterohemorrhagic-escherichia%C3%A2-coli-effector-tir-to-the-plasma-membrane-via-host-golgi-apparatus
#8
Chan Mao, Jiang Gu, Hai-Guang Wang, Yao Fang, Ping Yang, Bin Tang, Na Li, Ting-Ting Wang, Quan-Ming Zou, Qian Li
The translocated intimin receptor (Tir) is a canonical type III secretion system effector, secreted by the enterohemorrhagic Escherichia coli (E. coli). This receptor alters the regular cellular processing of host cells, to promote intracellular bacterial replication and evasion of the host immune system. Tir is translocated and integrated into the host cell plasma membrane, a process required for its pathogenic activity in these cells, however, the underlying mechanisms of how this occurs remain to be elucidated...
June 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28623321/dysbiosis-in-chronic-periodontitis-key-microbial-players-and-interactions-with-the-human-host
#9
Zhi-Luo Deng, Szymon P Szafrański, Michael Jarek, Sabin Bhuju, Irene Wagner-Döbler
Periodontitis is an extremely prevalent disease worldwide and is driven by complex dysbiotic microbiota. Here we analyzed the transcriptional activity of the periodontal pocket microbiota from all domains of life as well as the human host in health and chronic periodontitis. Bacteria showed strong enrichment of 18 KEGG functional modules in chronic periodontitis, including bacterial chemotaxis, flagellar assembly, type III secretion system, type III CRISPR-Cas system, and two component system proteins. Upregulation of these functions was driven by the red-complex pathogens and candidate pathogens, e...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622339/the-chlamydia-trachomatis-type-iii-secretion-substrates-ct142-ct143-and-ct144-are-secreted-into-the-lumen-of-the-inclusion
#10
Maria da Cunha, Sara V Pais, Joana N Bugalhão, Luís Jaime Mota
Chlamydia trachomatis is a human bacterial pathogen causing ocular and genital infections. It multiplies exclusively within an intracellular membrane-bound vacuole, the inclusion, and uses a type III secretion system to manipulate host cells by injecting them with bacterially-encoded effector proteins. In this work, we characterized the expression and subcellular localization in infected host cells of the C. trachomatis CT142, CT143, and CT144 proteins, which we previously showed to be type III secretion substrates...
2017: PloS One
https://www.readbyqxmd.com/read/28620361/genome-sequencing-of-ralstonia-solanacearum-cqps-1-a-phylotype-i-strain-collected-from-a-highland-area-with-continuous-cropping-of-tobacco
#11
Ying Liu, Yuanman Tang, Xiyun Qin, Liang Yang, Gaofei Jiang, Shili Li, Wei Ding
Ralstonia solanacearum, an agent of bacterial wilt, is a highly variable species with a broad host range and wide geographic distribution. As a species complex, it has extensive genetic diversity and its living environment is polymorphic like the lowland and the highland area, so more genomes are needed for studying population evolution and environment adaptation. In this paper, we reported the genome sequencing of R. solanacearum strain CQPS-1 isolated from wilted tobacco in Pengshui, Chongqing, China, a highland area with severely acidified soil and continuous cropping of tobacco more than 20 years...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28620360/cytr-homolog-of-pectobacterium-carotovorum-subsp-carotovorum-controls-air-liquid-biofilm-formation-by-regulating-multiple-genes-involved-in-cellulose-production-c-di-gmp-signaling-motility-and-type-iii-secretion-system-in-response-to-nutritional-and-environmental
#12
M M Haque, M M H Oliver, Kamrun Nahar, Mohammad Z Alam, Hisae Hirata, Shinji Tsuyumu
Pectobacterium carotovorum subsp. carotovorum [Pcc (formerly Erwinia carotovora subsp. carotovora)] PC1 causes soft-rot disease in a wide variety of plant species by secreting multiple pathogenicity-related traits. In this study, regulatory mechanism of air-liquid (AL) biofilm formation was studied using a cytR homolog gene deletion mutant (ΔcytR) of Pcc PC1. Compared to the wild type (Pcc PC1), the ΔcytR mutant produced fragile and significantly (P < 0.001) lower amounts of AL biofilm on salt-optimized broth plus 2% glycerol (SOBG), yeast peptone dextrose adenine, and also on King's B at 27°C after 72 h incubation in static condition...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28619883/spatiotemporal-monitoring-of-pseudomonas-effectors-via-type-iii-secretion-using-split-fluorescent-protein-fragments
#13
Eunsook Park, Hye-Young Lee, Jongchan Woo, Doil Choi, Savithramma P Dinesh-Kumar
Pathogenic gram-negative bacteria cause serious diseases in animals and plants. These bacterial pathogens use the type III secretion system (T3SS) to deliver effector proteins into host cells; these effectors then localize to different subcellular compartments to attenuate immune responses by altering biological processes of the host cells. The fluorescent protein (FP)-based approach to monitor effectors secreted from bacteria into the host cells is not possible because the folded FP prevents effector delivery through the T3SS...
June 14, 2017: Plant Cell
https://www.readbyqxmd.com/read/28611956/comparative-genomic-and-phylogenetic-analysis-of-a-shiga-toxin-producing-shigella-sonnei-stss-strain
#14
Domonkos Sváb, Balázs Bálint, Bálint Vásárhelyi, Gergely Maróti, István Tóth
Shigella strains are important agents of bacillary dysentery, and in recent years Shigella sonnei has emerged as the leading cause of shigellosis in industrialized and rapidly developing countries. More recently, several S. sonnei and Shigella flexneri strains producing Shiga toxin (Stx) have been reported from sporadic cases and from an outbreak in America. In the present study we aimed to shed light on the evolution of a recently identified Shiga toxin producing S. sonnei (STSS) isolated in Europe. Here we report the first completely assembled whole genome sequence of a multidrug resistant (MDR) Stx-producing S...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28606056/comparative-genome-and-transcriptome-analysis-reveals-distinctive-surface-characteristics-and-unique-physiological-potentials-of-pseudomonas-aeruginosa-atcc-27853
#15
Huiluo Cao, Yong Lai, Salim Bougouffa, Zeling Xu, Aixin Yan
BACKGROUND: Pseudomonas aeruginosa ATCC 27853 was isolated from a hospital blood specimen in 1971 and has been widely used as a model strain to survey antibiotics susceptibilities, biofilm development, and metabolic activities of Pseudomonas spp.. Although four draft genomes of P. aeruginosa ATCC 27853 have been sequenced, the complete genome of this strain is still lacking, hindering a comprehensive understanding of its physiology and functional genome. RESULTS: Here we sequenced and assembled the complete genome of P...
June 12, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28602608/multistage-vaccines-containing-outer-membrane-type-iii-secretion-system-and-inclusion-membrane-proteins-protects-against-a-chlamydia-genital-tract-infection-and-pathology
#16
Connor P O'Meara, Charles W Armitage, Dean W Andrew, Avinash Kollipara, Nils Y Lycke, Andrew A Potter, Volker Gerdts, Nikolai Petrovsky, Kenneth W Beagley
Pathogens with a complex lifecycles can effectively evade host immunity in part due to each developmental stage expressing unique sets of antigens. Multisubunit vaccines incorporating signature antigens reflecting distinct developmental stages (multistage vaccines) have proven effective against viral, bacterial and parasitic infection at preventing pathogen evasion of host immunity. Chlamydia trachomatis is characterized by a biphasic extra/intracellular developmental cycle and an acute/persistent (latent) metabolic state; hence a multistage vaccine may prevent immune evasion and enhance clearance...
June 8, 2017: Vaccine
https://www.readbyqxmd.com/read/28600390/direct-and-indirect-visualization-of-bacterial-effector-delivery-into-diverse-plant-cell-types-during-infection
#17
Elizabeth Henry, Tania Y Toruño, Alain Jauneau, Laurent Deslandes, Gitta Laurel Coaker
To cause disease, diverse pathogens deliver effector proteins into host cells. Pathogen effectors can inhibit defense responses, alter host physiology, and represent important cellular probes to investigate plant biology. However, effector function and localization have primarily been investigated after overexpression in planta. Visualizing effector delivery during infection is challenging due to the plant cell wall, autofluorescence, and low effector abundance. Here, we utilized a GFP strand system to directly visualize bacterial effectors delivered into plant cells through the Type III secretion system...
June 9, 2017: Plant Cell
https://www.readbyqxmd.com/read/28593198/biphasic-metabolism-and-host-interaction-of-a-chlamydial-symbiont
#18
Lena König, Alexander Siegl, Thomas Penz, Susanne Haider, Cecilia Wentrup, Julia Polzin, Evelyne Mann, Stephan Schmitz-Esser, Daryl Domman, Matthias Horn
Chlamydiae are obligate intracellular bacteria comprising well-known human pathogens and ubiquitous symbionts of protists, which are characterized by a unique developmental cycle. Here we comprehensively analyzed gene expression dynamics of Protochlamydia amoebophila during infection of its Acanthamoeba host by RNA sequencing. This revealed a highly dynamic transcriptional landscape, where major transcriptional shifts are conserved among chlamydial symbionts and pathogens. Our data served to propose a time-resolved model for type III protein secretion during the developmental cycle, and we provide evidence for a biphasic metabolism of P...
May 2017: MSystems
https://www.readbyqxmd.com/read/28591848/comparative-genomics-of-free-living-gamma-proteobacteria-pathogenesis-related-genes-or-interaction-related-genes
#19
Mirna Vázquez-Rosas-Landa, Gabriel Yaxal Ponce-Soto, Luis E Eguiarte, V Souza
Bacteria have numerous strategies to interact with them and with their environment. However, genes associated with these interactions are usually cataloged as pathogenic. To understand the role that these genes have not only in pathogenesis but also in bacterial interactions, we compared the genomes of eight bacteria from human-impacted environments with free-living bacteria from the Cuatro Ciénegas Basin (CCB), a relatively pristine oligotrophic site. Fifty-one genomes from CCB bacteria, including Pseudomonas, Vibrio, Photobacterium, and Aeromonas were analyzed...
June 7, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28588224/the-salicylidene-acylhydrazide-inp0341-attenuates-pseudomonas-aeruginosa-virulence-in-vitro-and-in-vivo
#20
Pia Uusitalo, Ulrik Hägglund, Elin Rhöös, Henrik Scherman Norberg, Mikael Elofsson, Charlotta Sundin
Pseudomonas aeruginosa is an opportunistic pathogen that can be very hard to treat because of high resistance to different antibiotics and alternative treatment regimens are greatly needed. An alternative or a complement to traditional antibiotic is to inhibit virulence of the bacteria. The salicylidene acylhydrazide, INP0341, belongs to a class of compounds that has previously been shown to inhibit virulence in a number of Gram-negative bacteria. In this study, the virulence blocking effect of INP0341 on P...
June 7, 2017: Journal of Antibiotics
keyword
keyword
61544
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"