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Lps challenge model

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https://www.readbyqxmd.com/read/28811580/%C3%AE-eta-2-glycoprotein-i-protects-mice-against-gram-negative-septicaemia-in-a-sexually-dimorphic-manner
#1
Fatima El-Assaad, Miao Qi, Alice Kizny Gordon, Jian Qi, Shangwen Dong, Freda Passam, James Crofton Weaver, Bill Giannakopoulos, Steven Anthony Krilis
The immune responses of males and females to bacterial infections display differences. The mechanisms that underlie this sexual dimorphism are multifactorial. Lipopolysaccharide (LPS) contributes to the pathogenesis of endotoxaemia. We have previously demonstrated that the plasma protein beta-2 glycoprotein-1 (β2GPI) reduces LPS-induced inflammation in male mice. In the present study using a more robust infection model of septicaemia the role of β2GPI is examined in both male and female wild type (WT) and β2GPI deficient (β2GPI(-/-)) mice challenged with Escherichia coli (E...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28807602/neutralization-of-interferon-%C3%AE-reverts-clinical-and-laboratory-features-in-a-mouse-model-of-macrophage-activation-syndrome
#2
Giusi Prencipe, Ivan Caiello, Antonia Pascarella, Alexei A Grom, Claudia Bracaglia, Laurence Chatel, Walter G Ferlin, Emiliano Marasco, Raffaele Strippoli, Cristina de Min, Fabrizio De Benedetti
BACKGROUND: The pathogenesis of macrophage activation syndrome (MAS) is not clearly understood: a large body of evidence supports the involvement of mechanisms similar to those implicated in primary hemophagocytic lymphohistiocytosis. OBJECTIVE: To investigate the pathogenic role of interferon-γ (IFNγ) and the therapeutic efficacy of IFNγ neutralization in an animal model of macrophage activation syndrome. METHODS: We used a MAS model established in mice transgenic for human interleukin-6 (IL-6TG) challenged with LPS (MAS mice)...
August 11, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28801676/hyaluronic-acid-coatings-as-a-simple-and-efficient-approach-to-improve-msc-homing-toward-the-site-of-inflammation
#3
Bruna Corradetti, Francesca Taraballi, Jonathan O Martinez, Silvia Minardi, Nupur Basu, Guillermo Bauza, Michael Evangelopoulos, Sebastian Powell, Claudia Corbo, Ennio Tasciotti
A major challenge in regenerative medicine is to improve therapeutic cells' delivery and targeting using an efficient and simple protocol. Mesenchymal stem cells (MSC) are currently employed for the treatment of inflammatory-based diseases, due to their powerful immunosoppressive potential. Here we report a simple and versatile method to transiently overexpress the hyaluronic acid (HA) receptor, CD44, on MSC membranes, to improve their homing potential towards an inflammatory site without affecting their behavior...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28801231/anti-inflammatory-chromatinscape-suggests-alternative-mechanisms-of-glucocorticoid-receptor-action
#4
Kyu-Seon Oh, Heta Patel, Rachel A Gottschalk, Wai Shing Lee, Songjoon Baek, Iain D C Fraser, Gordon L Hager, Myong-Hee Sung
Despite the widespread use of glucocorticoids (GCs), their anti-inflammatory effects are not understood mechanistically. Numerous investigations have examined the effects of glucocorticoid receptor (GR) activation prior to inflammatory challenges. However, clinical situations are emulated by a GC intervention initiated in the midst of rampant inflammatory responses. To characterize the effects of a late GC treatment, we profiled macrophage transcriptional and chromatinscapes with Dexamethasone (Dex) treatment before or after stimulation by lipopolysaccharide (LPS)...
August 15, 2017: Immunity
https://www.readbyqxmd.com/read/28800499/effects-of-preimplantation-factor-on-interleukin-6-and-prostaglandin-f2%C3%AE-and-e2-in-the-bovine-endometrium
#5
Ruth E Wonfor, Manuela Natoli, Michael T Rose, Deborah M Nash
Preimplantation factor (PIF) is a pregnancy specific peptide with immune modulatory properties exerted on the human endometrium. Viable bovine embryos secrete PIF, but its effect on the bovine endometrial immune response is unknown, both in native and inflammatory stimulated endometrial tissue. An ex vivo bovine endometrial tissue culture model was used with lipopolysaccharide (LPS) as an inflammatory stimulant. The effect of synthetic PIF (sPIF) was assessed, in three separate experiments, on the secretion or mRNA expression of essential prostaglandins and cytokines...
August 2, 2017: Theriogenology
https://www.readbyqxmd.com/read/28794232/resolvin-d1-improves-the-resolution-of-inflammation-via-activating-nf-%C3%AE%C2%BAb-p50-p50-mediated-cyclooxygenase-2-expression-in-acute-respiratory-distress-syndrome
#6
Ye Gao, Huawei Zhang, Lingchun Luo, Jing Lin, Dan Li, Sisi Zheng, Hua Huang, Songfan Yan, Jingxiang Yang, Yu Hao, Hui Li, Fang Gao Smith, Shengwei Jin
Acute respiratory distress syndrome (ARDS) is a severe illness characterized by uncontrolled inflammation. The resolution of inflammation is a tightly regulated event controlled by endogenous mediators, such as resolvin D1 (RvD1). Cyclooxygenase-2 (COX-2) has been reported to promote inflammation, along with PGE2, in the initiation of inflammation, as well as in prompting resolution, with PGD2 acting in the later phase of inflammation. Our previous work demonstrated that RvD1 enhanced COX-2 and PGD2 expression to resolve inflammation...
August 9, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28763157/a-unifying-mathematical-model-of-lipid-droplet-metabolism-reveals-key-molecular-players-in-the-development-of-hepatic-steatosis
#7
Christin Wallstab, Dimitra Eleftheriadou, Theresa Schulz, Georg Damm, Daniel Seehofer, Jürgen Borlak, Hermann-Georg Holzhütter, Nikolaus Berndt
The liver responds to elevated plasma concentrations of free fatty acids (FFAs) with an enhanced uptake of FFAs and their esterification to triacylglycerol (TAG). On the long term this may result in massive hepatic TAG accumulation called steatosis hepatitis. In hepatocytes, the poor water-soluble TAG is packed in specialized organelles: Lipid droplets (LDs) serving as transient cellular deposit and lipoproteins (LPs) transporting TAG and cholesterol esters to extra-hepatic tissues. The dynamics of these organelles is controlled by a variety of regulatory surface proteins (RSPs)...
August 1, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28762847/inhibition-of-nlrp3-inflammasome-a-new-protective-mechanism-of-cinnamaldehyde-in-endotoxin-poisoning-of-mice
#8
Feng Xu, Feng Wang, Taoqun Wen, Wentao Sang, Dejian Wang, Nan Zeng
CONTEXT: Cinnamaldehyde (CA) has a protective effect in endotoxin poisoning of mice, but there is no direct evidence for the protective effect of CA through inhibition of NLRP3 inflammasome activation in endotoxin poisoning of mice. OBJECTIVE: We aimed to investigate the protective mechanism of CA in endotoxin poisoned mice through NLRP3 inflammasome. MATERIALS AND METHODS: First, we evaluated the anti-inflammatory effect of CA in phorbol-12-myristate acetate-differentiated THP-1 cells through the NLRP3 inflammasome...
August 1, 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/28760701/repeated-daily-administration-of-increasing-doses-of-lipopolysaccharide-provides-a-model-of-sustained-inflammation-induced-depressive-like-behaviour-in-mice-that-is-independent-of-the-nlrp3-inflammasome
#9
Robin A Wickens, Luc Ver Donck, Amanda B MacKenzie, Sarah J Bailey
Mounting preclinical evidence has implicated the NLRP3 inflammasome in depression-related behaviours elicited by chronic stress or acute lipopolysaccharide (LPS) challenge. However, the relevance of acute LPS as a model of depression has been questioned and behavioural time-courses of its effects can be inconsistent. The aims of this study were (1) to develop a novel protocol for repeated daily LPS administration and (2) to use this model to assess the involvement of NLRP3 inflammasome signalling in sustained inflammation-induced depressive-like behaviour in adult C57BL/6J mice deficient in NLRP3...
July 28, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28759530/sevoflurane-attenuates-systemic-inflammation-compared-with-propofol-but-does-not-modulate-neuro-inflammation-a-laboratory-rat-study
#10
Beatrice Beck-Schimmer, Lukas Baumann, Tanja Restin, Philipp Eugster, Melanie Hasler, Christa Booy, Martin Schläpfer
BACKGROUND: Septic encephalopathy is believed to be a result of neuro-inflammation possibly triggered by endotoxins, such as lipopolysaccharides (LPS). Modulation of the immune system is a property of volatile anaesthetics. OBJECTIVE: We aimed to investigate the systemic and cerebral inflammatory response in a LPS-induced sepsis model in rats. We compared two different sedation strategies, intravenous propofol and the volatile anaesthetic sevoflurane, with the hypothesis that the latter may attenuate neuro-inflammatory processes...
July 28, 2017: European Journal of Anaesthesiology
https://www.readbyqxmd.com/read/28755942/regulation-of-inflammation-antioxidant-production-and-methyl-carbon-metabolism-during-methionine-supplementation-in-lipopolysaccharide-challenged-neonatal-bovine-hepatocytes
#11
Qian Zhang, Heather M White
Supplementation of methionine (Met) may improve immunometabolic status, specifically during a period of inflammatory stress. The aim of the present study was to establish an inflammation model using primary neonatal bovine hepatocytes and to examine the effects of increasing concentrations of dl-Met and a maintained Met to lysine (Lys) ratio on hepatocyte inflammatory responses, antioxidant production, and Met metabolism during lipopolysaccharide (LPS) challenge. Hepatocytes isolated from 4 calves were maintained as monolayer cultures and exposed to 0, 10, or 40 µMdl-Met and 100 µM Lys (0Met100Lys, 10Met100Lys, or 40Met100Lys) or 10 µMdl-Met and 25 µM Lys (10Met25Lys)...
July 26, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28743337/-effect-of-multidrug-resistant-associated-protein-4-overexpression-on-lipopolysaccharide-induced-vascular-endothelial-hyperpermeability-and-its-mechanism
#12
Wenfang Xia, Huanming Zhang, Yanlei Zheng, Qingshan Zhou, Bin Su
OBJECTIVE: To investigate the effect of multidrug resistance protein 4 (MRP4) overexpression on lipopolysaccharide (LPS)-induced vascular endothelial hyperpermeability of rat pulmonary micro-vascular endothelial cells (PMVECs) and its molecule mechanism. METHODS: Three to six passages of PMVECs were cultured in vitro, and they were divided into three groups: the cells in LPS group were only challenged by LPS 10 μg/mL after being cultured in serum-free medium for 24 hours; the cells in Ad-shRNA and Ad-MRP4 groups were infected with the empty virus control or recombinant adenovirus expressing MRP4 for 2 hours, and then were cultured in serum-free medium for 24 hours followed by stimulation of LPS 10 μg/mL...
July 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28739513/intrauterine-inflammation-induces-sex-specific-effects-on-neuroinflammation-white-matter-and-behavior
#13
Ryan Makinson, Kelsey Lloyd, Aditya Rayasam, Sarah McKee, Amy Brown, Guillermo Barila, Nicola Grissom, Robert George, Matt Marini, Zsuzsanna Fabry, Michal Elovitz, Teresa M Reyes
Exposure to inflammation during pregnancy has been linked to adverse neurodevelopmental consequences for the offspring. One common route through which a developing fetus is exposed to inflammation is with intrauterine inflammation. To that end, we utilized an animal model of intrauterine inflammation (IUI; intrauterine lipopolysaccharide (LPS) administration, 50 µg, E15) to assess placental and fetal brain inflammatory responses, white matter integrity, anxiety-related behaviors (elevated zero maze, light dark box, open field), microglial counts, and the CNS cytokine response to an acute injection of LPS in both males and females...
July 21, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28737175/natural-variation-of-macrophage-activation-as-disease-relevant-phenotype-predictive-of-inflammation-and-cancer-survival
#14
Konrad Buscher, Erik Ehinger, Pritha Gupta, Akula Bala Pramod, Dennis Wolf, George Tweet, Calvin Pan, Charles D Mills, Aldons J Lusis, Klaus Ley
Although mouse models exist for many immune-based diseases, the clinical translation remains challenging. Most basic and translational studies utilize only a single inbred mouse strain. However, basal and diseased immune states in humans show vast inter-individual variability. Here, focusing on macrophage responses to lipopolysaccharide (LPS), we use the hybrid mouse diversity panel (HMDP) of 83 inbred strains as a surrogate for human natural immune variation. Since conventional bioinformatics fail to analyse a population spectrum, we highlight how gene signatures for LPS responsiveness can be derived based on an Interleukin-12β and arginase expression ratio...
July 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28736267/gamma-tocopherol-enriched-supplement-reduces-sputum-eosinophilia-and-endotoxin-induced-sputum-neutrophilia-in-volunteers-with-asthma
#15
Allison J Burbank, Charity G Duran, Yinghao Pan, Patricia Burns, Susan Jones, Qing Jiang, Chao Yang, Sha'Leema Jenkins, Heather Wells, Neil Alexis, Mehmet Kesimer, William D Bennett, Haibo Zhou, David B Peden, Michelle L Hernandez
BACKGROUND: We and others have shown that the gamma tocopherol (γT) isoform of vitamin E has multiple anti-inflammatory and antioxidant actions and that γT supplementation reduces eosinophilic and endotoxin (LPS)-induced neutrophilic airway inflammation in animal models and healthy human volunteers. OBJECTIVE: We sought to determine whether γT supplementation reduces eosinophilic airway inflammation and acute neutrophilic response to inhaled LPS challenge in volunteers with asthma...
July 20, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28735521/myd88-erk-nfkb%C3%A2-pathways-and-pro-inflammatory-cytokines-release-in-periodontal-ligament-stem-cells-stimulated-by-porphyromonas%C3%A2-gingivalis
#16
Francesca Diomede, Maria Zingariello, Marcos F X B Cavalcanti, Ilaria Merciaro, Jacopo Pizzicannella, Natalia De Isla, Sergio Caputi, Patrizia Ballerini, Oriana Trubiani
The present study was aimed at investigating whether human Periodontal Ligament Stem Cells (hPDLSCs) were capable of sensing and reacting to lipopolysaccharide from Porphyromonas gingivalis (LPS-G) which is widely recognized as a major pathogen in the development and progression of periodontitis. At this purpose hPDLCs were stimulated with 5 μg/mL LPS-G various times and the expression of toll-like receptor 4 (TLR4) was evaluated. Toll-like receptors (TLRs) play an essential role in innate immune signaling in response to microbial infections, and in particular TLR4, type-I transmembrane proteins, has been shown recognizing LPS-G...
May 24, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28720697/inhibition-of-complement-c5-protects-against-organ-failure-and-reduces-mortality-in-a-baboon-model-of-escherichia-coli-sepsis
#17
Ravi Shankar Keshari, Robert Silasi, Narcis Ioan Popescu, Maulin Mukeshchandra Patel, Hala Chaaban, Cristina Lupu, K Mark Coggeshall, Tom Eirik Mollnes, Steven J DeMarco, Florea Lupu
Bacterial sepsis triggers robust activation of the complement system with subsequent generation of anaphylatoxins (C3a, C5a) and the terminal complement complex (TCC) that together contribute to organ failure and death. Here we tested the effect of RA101295, a 2-kDa macrocyclic peptide inhibitor of C5 cleavage, using in vitro whole-blood assays and an in vivo baboon model of Escherichia coli sepsis. RA101295 strongly inhibited E. coli-induced complement activation both in vitro and in vivo by blocking the generation of C5a and the soluble form of TCC, sC5b-9...
July 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28707482/peripheral-inflammation-apolipoprotein-e4-and-amyloid-%C3%AE-interact-to-induce-cognitive-and-cerebrovascular-dysfunction
#18
Felecia M Marottoli, Yuriko Katsumata, Kevin P Koster, Riya Thomas, David W Fardo, Leon M Tai
Cerebrovascular dysfunction is rapidly reemerging as a major process of Alzheimer's disease (AD). It is, therefore, crucial to delineate the roles of AD risk factors in cerebrovascular dysfunction. While apolipoprotein E4 ( APOE4), Amyloid-β (Aβ), and peripheral inflammation independently induce cerebrovascular damage, their collective effects remain to be elucidated. The goal of this study was to determine the interactive effect of APOE4, Aβ, and chronic repeated peripheral inflammation on cerebrovascular and cognitive dysfunction in vivo...
July 2017: ASN Neuro
https://www.readbyqxmd.com/read/28707000/inducing-maternal-inflammation-promotes-leptin-production-in-offspring-but-does-not-improve-allergic-symptoms-in-a-mouse-model-of-allergic-rhinitis
#19
Atsuko Imai, Keiko Satoi, Eka Fujimoto, Kazuto Sato
AIMS: The intrauterine environment is considered to affect immunological development in fetus, leading to an increased risk of developing allergy. In particular, maternal lipopolysaccharides (LPS) administration might regulate the development of allergic disease in offspring. Several studies have shown that being obese relates to a higher prevalence of allergic diseases compared to normal weight. The present study explored the effects of inducing maternal inflammation with LPS before pregnancy on body weight, physical composition including body fat, adipokine production, and pathology of allergic rhinitis in offspring...
June 2017: Heliyon
https://www.readbyqxmd.com/read/28706439/genome-wide-retinal-transcriptome-analysis-of-endotoxin-induced-uveitis-in-mice-with-next-generation-sequencing
#20
Yiguo Qiu, Peng Yu, Ru Lin, Xinyu Fu, Bingtao Hao, Bo Lei
PURPOSE: Endotoxin-induced uveitis (EIU) is a well-established mouse model for studying human acute inflammatory uveitis. The purpose of this study is to investigate the genome-wide retinal transcriptome profile of EIU. METHODS: The anterior segment of the mice was examined with a slit-lamp, and clinical scores were evaluated simultaneously. The histological changes in the posterior segment of the eyes were evaluated with hematoxylin and eosin (H&E) staining. A high throughput RNA sequencing (RNA-seq) strategy using the Illumina Hiseq 2500 platform was applied to characterize the retinal transcriptome profile from lipopolysaccharide (LPS)-treated and untreated mice...
2017: Molecular Vision
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