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Lps challenge model

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https://www.readbyqxmd.com/read/29243243/shikonin-inhibits-myeloid-differentiation-protein-2-to-prevent-lipopolysaccharide-induced-acute-lung-injury
#1
Yali Zhang, Tingting Xu, Zheer Pan, Xiangting Ge, Chuchu Sun, Chun Lu, Hongjin Chen, Zhongxiang Xiao, Bing Zhang, Yuanrong Dai, Guang Liang
BACKGROUND AND PURPOSE: Acute lung injury (ALI) is a challenging clinical syndrome which manifests as an acute inflammatory response. Myeloid differentiation protein 2 (MD2) has an important role in mediating lipopolysaccharide (LPS)-induced inflammation. Currently, there are no effective molecular-based therapies for ALI or viable biomarkers for predicting the severity of disease. Recent preclinical studies have shown that shikonin (SHI), a natural naphthoquinone, prevents LPS-inflammation...
December 15, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29238325/probiotic-bacteria-alter-pattern-recognition-receptor-expression-and-cytokine-profile-in-a-human-macrophage-model-challenged-with-candida-albicans-and-lipopolysaccharide
#2
Victor H Matsubara, Karin H Ishikawa, Ellen S Ando-Suguimoto, Bruno Bueno-Silva, Atlas E M Nakamae, Marcia P A Mayer
Probiotics are live microorganisms that confer benefits to the host health. The infection rate of potentially pathogenic organisms such as Candida albicans, the most common agent associated with mucosal candidiasis, can be reduced by probiotics. However, the mechanisms by which the probiotics interfere with the immune system are largely unknown. We evaluated the effect of probiotic bacteria on C. albicans challenged human macrophages. Macrophages were pretreated with lactobacilli alone (Lactobacillus rhamnosus LR32, Lactobacillus casei L324m, or Lactobacillus acidophilus NCFM) or associated with Escherichia coli lipopolysaccharide (LPS), followed by the challenge with C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29233990/balancing-mcr-1-expression-and-bacterial-survival-is-a-delicate-equilibrium-between-essential-cellular-defence-mechanisms
#3
Qiue Yang, Mei Li, Owen B Spiller, Diego O Andrey, Philip Hinchliffe, Hui Li, Craig MacLean, Pannika Niumsup, Lydia Powell, Manon Pritchard, Andrei Papkou, Yingbo Shen, Edward Portal, Kirsty Sands, James Spencer, Uttapoln Tansawai, David Thomas, Shaolin Wang, Yang Wang, Jianzhong Shen, Timothy Walsh
MCR-1 is a lipid A modifying enzyme that confers resistance to the antibiotic colistin. Here, we analyse the impact of MCR-1 expression on E. coli morphology, fitness, competitiveness, immune stimulation and virulence. Increased expression of mcr-1 results in decreased growth rate, cell viability, competitive ability and significant degradation in cell membrane and cytoplasmic structures, compared to expression of catalytically inactive MCR-1 (E246A) or MCR-1 soluble component. Lipopolysaccharide (LPS) extracted from mcr-1 strains induces lower production of IL-6 and TNF, when compared to control LPS...
December 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/29218090/mir-24-regulates-hepatocyte-apoptosis-via-bim-during-acute-liver-failure
#4
Zhiwen Feng, Zhi Li, Deming Zhu, Wei Ling, Lei Zheng, Liyong Pu, Lianbao Kong
Acuteliver failure (ALF) has a high mortality rate and is characterized by massive hepatocyte destruction. Although microRNAs (miRNAs) play an important role in manyliver diseases, the role of miRNAs in ALF development is unknown. In this study, the murine ALF model was induced by intraperitoneal injection of D-galactosamine/lipopolysaccharide (D-GalN/LPS). Compared with saline-treated mice, miR-24 was distinctly down-regulated post D-GalN/LPS challenge in vivo and D-galactosamine/tumor necrosis factor (D-GalN/TNF) challenge in vitro, which was confirmed by quantitative real-time polymerase chain reaction...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29214225/crocin-a-plant-derived-carotenoid-modulates-microglial-reactivity
#5
Mücella Arikan Yorgun, Khalid Rashid, Alexander Aslanidis, Charlotte Bresgen, Katharina Dannhausen, Thomas Langmann
Microglia activation plays an important role in immune responses in the CNS including the retina. Crocin, a plant-derived carotenoid, has been reported to possess anti-inflammatory, anti-apoptotic and anti-oxidative capacity in models of retinal damage and degeneration. If these neuroprotective effects could be mediated by direct modulation of microglial cells is unclear. Here, we examined the direct effects of crocin on key functions and pro-inflammatory gene expression in lipopolysaccharide (LPS)-activated BV-2 microglia...
December 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29209456/novel-glutathione-containing-dry-yeast-extracts-inhibit-eosinophilia-and-mucus-overproduction-in-a-murine-model-of-asthma
#6
Yun-Ho Kim, Yean-Jung Choi, Eun-Jung Lee, Min-Kyung Kang, Sin-Hye Park, Dong Yeon Kim, Hyeongjoo Oh, Sang-Jae Park, Young-Hee Kang
BACKGROUND/OBSECTIVE: Airway inflammation by eosinophils, neutrophils and alveolar macrophages is a characteristic feature of asthma that leads to pathological subepithelial thickening and remodeling. Our previous study showed that oxidative stress in airways resulted in eosinophilia and epithelial apoptosis. The current study investigated whether glutathione-containing dry yeast extract (dry-YE) ameliorated eosinophilia, goblet cell hyperplasia and mucus overproduction. MATERIALS/METHOD: This study employed 2 µg/mL lipopolysaccharide (LPS)- or 20 ng/mL eotaxin-1-exposed human bronchial epithelial cells and ovalbumin (OVA)-challenged mice...
December 2017: Nutrition Research and Practice
https://www.readbyqxmd.com/read/29208272/biosynthesis-of-cerium-oxide-nanoparticles-and-their-effect-on-lipopolysaccharide-lps-induced-sepsis-mortality-and-associated-hepatic-dysfunction-in-male-sprague-dawley-rats
#7
Guoqiang Chen, Yunfei Xu
The fetal/life challenging Sepsis is an urgent medical disaster which have developed "blood poisoning". The sepsis is one of the disorders of organs which cause death in patients. The investigational models that mimicchemical change of sepsis in human bodywereessential. We have synthesized CeO2 NPs using eco-friendly method using plant extract. The characterization of synthesized CeO2 NPs was studied using UV-Vis, FTIR, XRD, SEM, EDX and TEM analysis. The results were confirmed that the synthesized CeO2 was in nano size with spherical shape...
February 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29186870/dietary-supplementation-with-lactobacillus-casei-alleviates-lipopolysaccharide-induced-liver-injury-in-a-porcine-model
#8
Di Zhao, Tao Wu, Dan Yi, Lei Wang, Peng Li, Junmei Zhang, Yongqing Hou, Guoyao Wu
This study aims to determine whether Lactobacillus casei (L. casei) could relieve liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were randomly allocated into one of the three groups: control, LPS, and L. casei. The control and LPS groups were fed a corn- and soybean meal-based diet, whereas the L. casei group was fed the basal diet supplemented with 6 × 10⁶ cfu/g L. casei. On Day 31 of the trial, piglets in the LPS and L. casei groups received intraperitoneal administration of LPS (100 µg/kg body weight), while the control group received the same volume of saline...
November 26, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29174741/antidepressant-like-effect-of-zileuton-is-accompanied-by-hippocampal-neuroinflammation-reduction-and-creb-bdnf-upregulation-in-lipopolysaccharide-challenged-mice
#9
Dan-Dan Li, Hang Xie, Yi-Feng Du, Yan Long, Miranda N Reed, Mei Hu, Vishnu Suppiramaniam, Hao Hong, Su-Su Tang
BACKGROUND: Recent studies demonstrated beneficial effects of zileuton, a 5-lipoxygenase (5LO) inhibitor, on some brain diseases in animal models, but the role of zileuton in the depression remains unknown. METHODS: We investigated the effects of zileuton on depressive behaviors using tail suspension test (TST), forced swimming test (FST) and novelty-suppressed feeding test (NSFT) in mice injected with lipopolysaccharide (LPS). The 5LO level, activation of microglia, NF-κB p65, TNF-α, IL-1β, brain-derived neurotrophic factor (BDNF), and c-AMP response element-binding protein (CREB) were determined in the mouse hippocampus...
November 13, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29174411/gestational-intermittent-hypoxia-increases-susceptibility-to-neuroinflammation-and-alters-respiratory-motor-control-in-neonatal-rats
#10
REVIEW
Stephen M Johnson, Karanbir S Randhawa, Jenna J Epstein, Ellen Gustafson, Austin D Hocker, Adrianne G Huxtable, Tracy L Baker, Jyoti J Watters
Sleep disordered breathing (SDB) and obstructive sleep apnea (OSA) during pregnancy are growing health concerns because these conditions are associated with adverse outcomes for newborn infants. SDB/OSA during pregnancy exposes the mother and the fetus to intermittent hypoxia. Direct exposure of adults and neonates to IH causes neuroinflammation and neuronal apoptosis, and exposure to IH during gestation (GIH) causes long-term deficits in offspring respiratory function. However, the role of neuroinflammation in CNS respiratory control centers of GIH offspring has not been investigated...
November 22, 2017: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/29156799/asiatic-acid-protects-against-hepatic-ischemia-reperfusion-injury-by-inactivation-of-kupffer-cells-via-ppar%C3%AE-nlrp3-inflammasome-signaling-pathway
#11
Ying Xu, Jun Yao, Chen Zou, Heng Zhang, Shouliang Zhang, Jun Liu, Gui Ma, Pengcheng Jiang, Wenbo Zhang
Hepatic ischemia/reperfusion (I/R) contributes to major complications in clinical practice affecting perioperative morbidity and mortality. Recent evidence suggests the key role of nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain-containing 3 (NLRP3) inflammaosme activation on the pathogenesis of I/R injury. Asiatic acid (AA) is a pentacyclic triterpene derivative presented with versatile activities, including antioxidant, anti-inflammation and hepatoprotective effects. This study was designed to determine whether AA had potential hepatoprotective benefits against hepatic I/R injury, as well as to unveil the underlying mechanisms involved in the putative effects...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29152585/total-lipopolysaccharide-from-the-human-gut-microbiome-silences-toll-like-receptor-signaling
#12
Eva d'Hennezel, Sahar Abubucker, Leon O Murphy, Thomas W Cullen
Cohabitation of microbial communities with the host enables the formation of a symbiotic relationship that maintains homeostasis in the gut and beyond. One prevailing model suggests that this relationship relies on the capacity of host cells and tissues to remain tolerant to the strong immune stimulation generated by the microbiota such as the activation of Toll-like receptor 4 (TLR4) pathways by lipopolysaccharide (LPS). Indeed, gut microbial LPS is thought to be one of the most potent activators of innate immune signaling and an important mediator of the microbiome's influence on host physiology...
November 2017: MSystems
https://www.readbyqxmd.com/read/29149810/exacerbation-of-acute-traumatic-brain-injury-by-circulating-extracellular-vesicles
#13
Isla Hazleton, Abi Yates, Ashley Dale, Jay Roodselaar, Naveed Akbar, Marc Ruitenberg, Daniel C Anthony, Yvonne Couch
Inflammatory lesions in the brain activate a systemic acute phase response (APR), which is dependent on the release of extracellular vesicles (EVs) into the circulation. The resulting APR is responsible for regulating leukocyte mobilization and subsequent recruitment to the brain. Factors that either exacerbate or inhibit the APR will also exacerbate or inhibit CNS inflammation as a consequence, and have the potential to influence ongoing secondary damage. Here, we were interested to discover how the circulating EV population changes after traumatic brain injury (TBI) and how manipulation of the circulating EV pool impacts on the outcome of TBI...
November 17, 2017: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29140265/anti-inflammatory-potential-of-1-nitro-2-phenylethylene
#14
Michelle A Sugimoto, Márcia de Jesus Amazonas da Silva, Larissa Froede Brito, Rosivaldo Dos Santos Borges, Flávio Almeida Amaral, Ana Paula de Araujo Boleti, Maritza Echevarria Ordoñez, Jose Carlos Tavares, Lirlandia Pires Sousa, Emerson Silva Lima
Inflammation is a reaction of the host to infectious or sterile stimuli and has the physiological purpose of restoring tissue homeostasis. However, uncontrolled or unresolved inflammation can lead to tissue damage, giving rise to a plethora of chronic inflammatory diseases, including metabolic syndrome and autoimmunity pathologies with eventual loss of organ function. Beta-nitrostyrene and its derivatives are known to have several biological activities, including anti-edema, vasorelaxant, antiplatelet, anti-inflammatory, and anticancer...
November 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29126656/the-effect-of-i%C3%AE%C2%BAk-16-on-lipopolysaccharide-induced-impaired-monocytes
#15
Norman J Galbraith, Stephen Manek, Samuel Walker, Campbell Bishop, Jane V Carter, Meredith Cahill, Sarah A Gardner, Hiram C Polk, Susan Galandiuk
This study focuses on impaired monocyte function, which occurs in some patients after trauma, major elective surgery, or sepsis. This monocyte impairment increases the risk of secondary infection and death. We aimed to determine the influence IκK-16 had on monocytes using an ex-vivo model of human monocyte impairment. We included the effects of the well-studied comparators interferon-gamma (IFN-γ) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on impaired monocytes. Primary human monocytes were stimulated with 10ng/mL of lipopolysaccharide (LPS) for 16h and then challenged with 100ng/mL LPS to assess the monocyte inflammatory response...
October 28, 2017: Immunobiology
https://www.readbyqxmd.com/read/29116368/n-acetyl-cysteine-reverses-bio-behavioural-changes-induced-by-prenatal-inflammation-adolescent-methamphetamine-exposure-and-combined-challenges
#16
Twanette Swanepoel, Marisa Möller, Brian Herbert Harvey
RATIONALE: Schizophrenia is associated with prenatal inflammation and/or postnatal stressors such as drug abuse, resulting in immune-redox dysfunction. Antioxidants may offer therapeutic benefits. OBJECTIVES: The objective of this study is to investigate N-acetyl cysteine (NAC) as a therapeutic antioxidant to reverse schizophrenia-like bio-behavioural changes in rats exposed to maternal immune activation (MIA), adolescent methamphetamine (MA) or a combination thereof...
November 8, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/29107983/synthetic-host-defense-peptide-idr-1002-reduces-inflammation-in-pseudomonas-aeruginosa-lung-infection
#17
Kelli C Wuerth, Reza Falsafi, Robert E W Hancock
Pseudomonas aeruginosa is a frequent cause of lung infections, particularly in chronic infections in cystic fibrosis patients. However, treatment is challenging due to P. aeruginosa evasion of the host immune system and the rise of antibiotic resistant strains. Host defense peptides (HDPs) and synthetic derivatives called innate defense regulators (IDRs) have shown promise in several infection models as an alternative to antibiotic treatment. Here we tested peptide IDR-1002 against P. aeruginosa in vitro and in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/29101016/synthesis-and-evaluation-of-dual-crosslinked-alginate-microbeads
#18
Sami I Somo, Kelly Langert, Chin-Yu Yang, Marcella K Vaicik, Veronica Ibarra, Alyssa A Appel, Banu Akar, Ming-Huei Cheng, Eric M Brey
Alginate hydrogels have been investigated for a broad variety of medical applications. The ability to assemble hydrogels at neutral pH and mild temperatures makes alginate a popular choice for the encapsulation and delivery of cells and proteins. Alginate has been studied extensively for the delivery of islets as a treatment for type 1 diabetes. However, poor stability of the encapsulation systems after implantation remains a challenge. In this paper, alginate was modified with 2-aminoethyl methacrylate hydrochloride (AEMA) to introduce groups that can be photoactivated to generate covalent bonds...
October 31, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29081982/the-making-of-a-miscreant-tobacco-smoke-and-the-creation-of-pathogen-rich-biofilms
#19
Samir A Shah, Sukirth M Ganesan, Saradhadevi Varadharaj, Shareef M Dabdoub, John D Walters, Purnima S Kumar
We have previously reported that oral biofilms in clinically healthy smokers are pathogen-rich, and that this enrichment occurs within 24 h of biofilm formation. The present investigation aimed to identify a mechanism by which smoking creates this altered community structure. By combining in vitro microbial-mucosal interface models of commensal (consisting of Streptococcus oralis, Streptococcus sanguis, Streptococcus mitis, Actinomyces naeslundii, Neisseria mucosa and Veillonella parvula) and pathogen-rich (comprising S...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/29078089/stigmasterol-inhibits-lipopolysaccharide-induced-innate-immune-responses-in-murine-models
#20
Aaron O Antwi, David D Obiri, Newman Osafo, Arnold D Forkuo, Leslie B Essel
Stigmasterol is a naturally occurring steroid alcohol which occurs in vegetables, soya and a large variety of medicinal plants. Stigmasterol and other phytosterols have been documented as immunomodulators with huge therapeutic potential. We assessed the mitigating effect of stigmasterol on non-fatal and fatal innate immune responses in murine models after intraperitoneal challenge with an endotoxin, lipopolysaccharide, LPS. The effect of stigmasterol on LPS-induced febrile response, inflammatory cell proliferation, multiple organ damage and mortality were respectively investigated...
October 24, 2017: International Immunopharmacology
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