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https://www.readbyqxmd.com/read/28431078/effect-of-threonine-on-secretory-immune-system-using-a-chicken-intestinal-ex%C3%A2-vivo-model-with-lipopolysaccharide-challenge
#1
Q Zhang, X Chen, S D Eicher, K M Ajuwon, T J Applegate
Secretory IgA (sIgA) and its transcytosis receptor, polymeric immunoglobulin receptor (pIgR), along with mucus, form the first lines of intestinal defense. Threonine (Thr) is a major component of intestinal mucins and IgA, which are highly secreted under lipopolysaccharide (LPS) induced inflammation. In the current study, the effect of Thr on the secretory immune system was determined in an ex vivo chicken ileal explant model. Results showed that a 2-hour Thr-deprivation of culture medium induced a compensatory increase in the mRNA expression of interleukin-8 (IL-8), mucin 2 ( ), and IgA during LPS challenge, and this increase was suppressed with Thr addition to the media ( P  ≤ 0...
April 18, 2017: Poultry Science
https://www.readbyqxmd.com/read/28430654/alpha-ketoglutarate-suppresses-the-nf-%C3%AE%C2%BAb-mediated-inflammatory-pathway-and-enhances-the-pxr-regulated-detoxification-pathway
#2
Liuqin He, Huan Li, Niu Huang, Xihong Zhou, Junquan Tian, Tiejun Li, Jing Wu, Yanan Tian, Yulong Yin, Kang Yao
Alpha-ketoglutarate (AKG) is a critical nutritional factor in the maintenance of intestinal homeostasis. However, the relative mechanism of AKG has not been well understood. It was recently shown that the interaction between nuclear factor kappa B (NF-κB)-mediated inflammatory pathway and pregnane X receptor (PXR)-regulated detoxification pathway is a check and balance mechanism for keeping the homeostatic state of the intestine, preventing the onset of intestinal inflammation which may lead to cancer. In the current study we used lipopolysaccharide (LPS)-challenged piglet and intestinal porcine epithelial cells-J2 models to investigate the effects of dietary AKG supplementation on the intestinal immune system and PXR regulated target expression...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28429673/hydroxytyrosol-attenuates-lps-induced-acute-lung-injury-in-mice-by-regulating-autophagy-and-sirtuin-expression
#3
X Yang, T Jing, Y Li, Y He, B Wang, W Zhang, Y Xiao, W Wang, J Zhang, J Wei, R Lin
BACKGROUND: Recently, the effects of hydroxytyrosol on autophagy during acute lung injury (ALI) have drawn increasing attention. OBJECTIVE: We explored the underlying molecular mechanisms by which hydroxytyrosol exerts its anti-inflammatory effects in a murine model of ALI by up-regulating autophagy. METHODS: Male BALB/c mice, challenged with intranasal instillations of LPS, were treated with or without hydroxytyrosol (HT, 100 mg/kg, intragastrically) 1 h prior to LPS exposure...
April 21, 2017: Current Molecular Medicine
https://www.readbyqxmd.com/read/28424078/interleukin-6-displays-lung-anti-inflammatory-properties-and-exerts-protective-hemodynamic-effects-in-a-double-hit-murine-acute-lung-injury
#4
Guillaume Voiriot, Keyvan Razazi, Valérie Amsellem, Jeanne Tran Van Nhieu, Shariq Abid, Serge Adnot, Armand Mekontso Dessap, Bernard Maitre
BACKGROUND: Interleukin 6 (IL-6) is a predictive factor of poor prognosis in patients with acute respiratory distress syndrome (ARDS). However, its acute pulmonary hemodynamic effects and role in lung injury have not been investigated in a clinically relevant murine model of ARDS. METHODS: We used adult C57Bl6 wild-type (WT) and IL-6 knock-out (IL-6KO) mice. The animals received intravenous recombinant human IL-6 (rHuIL-6) or vehicle followed by intratracheal lipopolysaccharide (LPS) or saline before undergoing low tidal volume mechanical ventilation (MV) for 5 h...
April 19, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28421528/postnatal-lps-challenge-impacts-escape-learning-and-expression-of-plasticity-factors-mmp9-and-timp1-in-rats-effects-of-repeated-training
#5
Alexander Trofimov, Tatyana Strekalova, Niall Mortimer, Olga Zubareva, Alexander Schwarz, Evgeniy Svirin, Aleksei Umriukhin, Andrei Svistunov, Klaus-Peter Lesch, Victor Klimenko
Bacterial intoxication associated with inflammatory conditions during development can impair brain functions, in particular evolutionarily novel forms of memory, such as explicit learning. Little is known about the dangers of early-life inflammation on more basic forms of learning, for example, the acquisition of motor escape abilities, which are generally better preserved under pathological conditions. To address this limitation in knowledge, an inflammatory response was elicited in Wistar pups by lipopolysaccharide (LPS) injections (25 μg/kg) on postnatal days P15, P18 and P21...
April 18, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28420461/-%C3%AE-7-nicotinic-acetylcholine-receptor-agonist-attenuated-the-lipopolysaccharide-induced-inflammatory-response-via-inhibiting-the-activation-of-nuclear-factor-%C3%AE-b
#6
Xingxing Shi, Jianhua Yao, Cheng Wang, Xijing Zhang
OBJECTIVE: To investigate the effects of α7 nicotinic acetylcholine receptor (α7nAChR) on the inflammatory response induced by lipopolysaccharide (LPS) in RAW264.7 macrophages and its molecular mechanisms. METHODS: RAW264.7 macrophages were cultured in vitro. Inflammatory cell model was constructed by LPS stimulation. Cells were challenged by LPS (1, 10, 100 and 500 μg/L) for 5 hours or 100 μg/L LPS for 0, 2, 4, 8, 12, 24, 48 and 72 hours, and the release of tumor necrosis factor-α (TNF-α) was detected by the enzyme linked immunosorbent assay (ELISA)...
April 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28420140/oleuropein-decreases-cyclooxygenase-2-and-interleukin-17-expression-and-attenuates-inflammatory-damage-in-colonic-samples-from-ulcerative-colitis-patients
#7
Tiziana Larussa, Manuela Oliverio, Evelina Suraci, Marta Greco, Roberta Placida, Serena Gervasi, Raffaella Marasco, Maria Imeneo, Donatella Paolino, Luigi Tucci, Elio Gulletta, Massimo Fresta, Antonio Procopio, Francesco Luzza
Oleuropein (OLE) is the major phenolic secoiridoid of olive tree leaves, and its antioxidant and anti-inflammatory activities have been demonstrated in in vitro and in vivo animal models. The aim of this study was to investigate the activity of OLE in the colonic mucosa from patients with ulcerative colitis (UC). Biopsies obtained during colonoscopy from 14 patients with active UC were immediately placed in an organ culture chamber and challenged with lipopolysaccharide from Escherichia coli (EC-LPS) at 1 μg/mL in the presence or absence of 3 mM OLE...
April 15, 2017: Nutrients
https://www.readbyqxmd.com/read/28409189/daphnetin-reduces-endotoxin-lethality-in-mice-and-decreases-lps-induced-inflammation-in-raw264-7-cells-via-suppressing-jak-stats-activation-and-ros-production
#8
Lei Shen, Ting Zhou, Jing Wang, Xiumei Sang, Lei Lan, Lan Luo, Zhimin Yin
OBJECTIVE: Here, we used various approaches to investigate the suppressive role of daphnetin in LPS-induced inflammatory response, with the goal to understand the underlining molecular mechanism by which daphnetin regulated these processes. METHODS: We examined the survival rate and the lung injury in the mice model of LPS-induced endotoxemia. The production of pro-inflammatory factors including tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IL-6, nitric oxide (NO), and prostaglandin E2 (PGE2) was measured by ELISA and nitrite analysis, respectively...
April 13, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28405145/acanthopanax-senticosus-polysaccharides-induced-intestinal-tight-junction-injury-alleviation-via-inhibition-of-nf-%C3%AE%C2%BAb-mlck-pathway-in-a-mouse-endotoxemia-model
#9
Jie Han, Ji-Hong Li, Guang Bai, Guo-Shun Shen, Jing Chen, Jia-Nan Liu, Shuo Wang, Xian-Jun Liu
AIM: To examine the effects of Acanthopanax senticosus polysaccharides (ASPS) on intestinal tight junction (TJ) disruption and nuclear factor-kappa B (NF-κB)/myosin light chain kinase (MLCK) activation in endotoxemia. METHODS: BALB/C mice (6-8-weeks-old) received continuous intragastric gavage of ASPS for 7 d before injection of lipopolysaccharide (LPS), or received ASPS once after LPS injection. Blood and intestinal mucosal samples were collected 6 h after LPS challenge...
March 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28390976/rough-brucella-strain-rm57-is-attenuated-and-confers-protection-against-brucella-melitensis
#10
Yu Feng, Xiaowei Peng, Hui Jiang, Yong Peng, Liangquan Zhu, Jiabo Ding
Brucella melitensis (B. melitensis) is a facultative intracellular pathogen, which is the main epidemic strain in China. To overcome disadvantages of traditional live attenuated vaccines, in this study a rough mutant RM57 was induced from a B. melitensis isolate M1981. In order to uncover the reason of changes in the LPS of RM57, the nucleotide sequences and transcription levels of all known genes related to LPS synthesis were detected. As LPS plays an important role in outer membrane integrity, the sensitivities of RM57 to SDS and polymyxin B were examined...
April 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28386325/a-novel-acute-lethal-liver-injury-mouse-model-with-visualization-of-nf-%C3%AE%C2%BAb-activity-for-treatment-of-severe-acute-liver-injury
#11
Huanjin Liao, Jun Cai, Lifang Zhang, Yanxia Peng, Ping Wu, Tong Xie, Qingjun Pan
Acute lethal inflammation, especially that related to liver injury, is an important clinical issue. To date, however, there is no model that can be used to assess this serious condition. This study was designed to establish a novel lipopolysaccharide (LPS)/D-galactosamine (D-GalN)-induced acute lethal liver injury model in nuclear factor-κB (NF-κB) transgenic mice. The results show that a high dose of LPS (500 μg/kg) plus D-GalN (800 mg/kg) successfully established a novel mouse model of acute lethal liver injury with a lifespan of 8-10 h...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28385925/neutrophil-myeloperoxidase-diminishes-the-toxic-effects-and-mortality-induced-by-lipopolysaccharide
#12
Laurent L Reber, Caitlin M Gillis, Philipp Starkl, Friederike Jönsson, Riccardo Sibilano, Thomas Marichal, Nicolas Gaudenzio, Marion Bérard, Stephan Rogalla, Christopher H Contag, Pierre Bruhns, Stephen J Galli
Neutrophils have crucial antimicrobial functions but are also thought to contribute to tissue injury upon exposure to bacterial products, such as lipopolysaccharide (LPS). To study the role of neutrophils in LPS-induced endotoxemia, we developed a new mouse model, PMN(DTR) mice, in which injection of diphtheria toxin induces selective neutrophil ablation. Using this model, we found, surprisingly, that neutrophils serve to protect the host from LPS-induced lethal inflammation. This protective role was observed in conventional and germ-free animal facilities, indicating that it does not depend on a particular microbiological environment...
April 6, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28371849/therapeutic-inhibition-of-mir-375-attenuates-post-mi-inflammatory-response-and-left-ventricular-dysfunction-via-pdk-1-akt-signaling-axis
#13
Venkata Naga Srikanth Garikipati, Suresh Kumar Verma, Darukeshwara Jolardarashi, Zhongjian Cheng, Jessica Ibetti, Maria Cimini, Yan Tang, Mohsin Khan, Yujia Yue, Cindy Benedict, Emily Nickoloff, May M Trungcao, Erhe Gao, Prasanna Krishnamurthy, David A Goukassian, Walter J Koch, Raj Kishore
Aims: Increased miR-375 levels has been implicated in rodent models of myocardial infarction (MI) and with patients with heart failure. However, no prior study had established a therapeutic role of miR-375 in ischemic myocardium. Therefore, we assessed whether inhibition of MI-induced miR-375 by LNA anti-miR -375 can improve recovery after acute MI. Methods and Results: Ten weeks old mice were treated with either control or LNA anti miR-375 after induction of MI by LAD ligation...
March 28, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28342021/establishment-of-immortalized-mouse-intestinal-epithelial-cells-line-and-study-of-effects-of-arg-arg-on-inflammatory-response
#14
Kang Zhan, Maocheng Jiang, Yannan Sui, Kang Yan, Miao Lin, Guoqi Zhao
Primary mouse intestinal epithelial cells (MIEs) are not ideal models for long-term culture in vitro and a limited amount of approximate three generations. In addition, the mechanism that arginine-arginine dipeptide (Arg-Arg) regulates mouse intestinal inflammatory response remains unknown. Therefore, the aim of this study was to establish immortal MIEs and study the effects of Arg-Arg on inflammatory response after challenging the MIEs with lipopolysaccharide (LPS) or staphylococcal enterotoxin C (rSEC). Our data showed that immortalized MIEs could be cultured over 100 generations...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28340574/myocyte-enhancer-factor-2d-provides-a-cross-talk-between-chronic-inflammation-and-lung-cancer
#15
Hai-Xing Zhu, Lin Shi, Yong Zhang, Yi-Chun Zhu, Chun-Xue Bai, Xiang-Dong Wang, Jie-Bai Zhou
BACKGROUND: Lung cancer is the leading cause of cancer-related morbidity and mortality worldwide. Patients with chronic respiratory diseases, such as chronic obstructive pulmonary disease (COPD), are exposed to a higher risk of developing lung cancer. Chronic inflammation may play an important role in the lung carcinogenesis among those patients. The present study aimed at identifying candidate biomarker predicting lung cancer risk among patients with chronic respiratory diseases. METHODS: We applied clinical bioinformatics tools to analyze different gene profile datasets with a special focus on screening the potential biomarker during chronic inflammation-lung cancer transition...
March 24, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28335090/in-ovo-vaccination-using-eimeria-profilin-and-clostridium-perfringens-netb-proteins-in-montanide-ims-adjuvant-increases-protective-immunity-against-experimentally-induced-necrotic-enteritis
#16
Hyun Soon Lillehoj, Seung Ik Jang, Alfredo Panebra, Erik Peter Lillehoj, Laurent Dupuis, Juliette B Arous, Seung Kyoo Lee, Sung Taek Oh
Objective: The effects of vaccinating 18-day-old chicken embryos with the combination of recombinant Eimeria profilin plus Clostridium perfringens NetB proteins mixed in the Montanide IMS adjuvant on the chicken immune response to necrotic enteritis (NE) were investigated using an E. maxima/C. perfringens co-infection NE disease model that we previously developed. Methods: Eighteen-day-old broiler embryos were injected with 100 μl of PBS, profilin, profilin plus NetB, profilin plus NetB/Montanide adjuvant (IMS 106), and profilin plus Net-B/Montanide adjuvant (IMS 101)...
March 21, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28333981/lipopolysaccharide-induced-endotoxemia-in-corn-oil-preloaded-mice-causes-an-extended-course-of-lung-injury-and-repair-and-pulmonary-fibrosis-a-translational-mouse-model-of-acute-respiratory-distress-syndrome
#17
Chaomin Wu, Colin E Evans, Zhiyu Dai, Xiaojia Huang, Xianming Zhang, Hua Jin, Guochang Hu, Yuanlin Song, You-Yang Zhao
Acute respiratory distress syndrome (ARDS) is characterized by acute hypoxemia respiratory failure, bilateral pulmonary infiltrates, and pulmonary edema of non-cardiac origin. Effective treatments for ARDS patients may arise from experimental studies with translational mouse models of this disease that aim to delineate the mechanisms underlying the disease pathogenesis. Mouse models of ARDS, however, can be limited by their rapid progression from injured to recovery state, which is in contrast to the course of ARDS in humans...
2017: PloS One
https://www.readbyqxmd.com/read/28323124/cd44-is-a-key-player-in-non-alcoholic-steatohepatitis
#18
Stéphanie Patouraux, Déborah Rousseau, Stéphanie Bonnafous, Cynthia Lebeaupin, Carmelo Luci, Clémence M Canivet, Anne-Sophie Schneck, Adeline Bertola, Marie-Christine Saint-Paul, Antonio Iannelli, Jean Gugenheim, Rodolphe Anty, Albert Tran, Béatrice Bailly-Maitre, Philippe Gual
BACKGROUND: CD44 is expressed on many immune cells that contribute to inflammation. It has been shown, mainly through animal data, that CD44 regulates adipose tissue inflammation in obesity and hepatic leukocyte recruitment in a lithogenic context. However, its role in hepatic inflammation in a mouse model of steatohepatitis and its relevance in humans have not yet been investigated. METHODS: The role of CD44 was evaluated in CD44-/- mice and after injections of an αCD44 antibody in Wild-type mice challenged with a methionine- and choline-deficient diet (MCDD)...
March 16, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28319310/cxcl4-is-a-novel-nickel-binding-protein-and-augments-nickel-allergy
#19
Toshinobu Kuroishi, Kanan Bando, Yukinori Tanaka, Kaori Shishido, Masayuki Kinbara, Tomohisa Ogawa, Koji Muramoto, Yasuo Endo, Shunji Sugawara
BACKGROUND: Nickel (Ni) is the most frequent metal allergen and induces a TH1 -dependent type-IV allergy. Although Ni(2+) is considered to bind to endogenous proteins, it currently remains unclear whether these Ni-binding proteins are involved in Ni allergy in vivo. We previously reported the adjuvant effects of lipopolysaccharide (LPS) in a Ni allergy mouse model. Since LPS induces a number of inflammatory mediators, we hypothesized that Ni-binding protein(s) are also induced by LPS...
March 20, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28302538/protective-effects-of-garcinol-against-neuropathic-pain-evidence-from-in-vivo-and-in-vitro-studies
#20
Yi-Wei Wang, Xiang Zhang, Chun-Long Chen, Qing-Zhen Liu, Jia-Wen Xu, Qing-Qing Qian, Wei-Yan Li, Yan-Ning Qian
Neuroinflammatory processes have a vital role in the pathogenesis of neuropathic pain. Garcinol, harvested from Garcinia indica, is known to exert potent anti-inflammatory properties. Recent studies have indicated that Garcinol may inhibit activation of nuclear factor-κB (NF-κB) by inhibiting NF-κB/p65 acetylation. These findings prompted us to evaluate the protective effects of Garcinol in the lumbar fifth spinal nerve ligation (SNL)-induced rat model of neuropathic pain and Lipopolysaccharide(LPS)-stimulated primary cultured microglia...
March 14, 2017: Neuroscience Letters
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