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Lps challenge model

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https://www.readbyqxmd.com/read/29456654/establishment-of-different-experimental-asthma-models-in-mice
#1
Qian-Lin Yu, Zhangbo Chen
Asthma is a complex disease. The heterogeneity of airway inflammation during asthma indicates there are different mechanisms involved. In order to further study the mechanisms of asthma, different mouse models were established to mimic corresponding subtypes of asthma in clinic. Eosinophilic asthma was established by intraperitoneal injections of ovalbumin (OVA) on day 0 and day 7, followed by inhalation of aerosolized OVA on days 14-17. Neutrophilic asthma was established by transtracheal administration of a high dose of lipopolysaccharide (LPS; 10 µg) on days 15 and 17 in combination with OVA sensitization and challenge as described previously...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29450541/induction-of-ocular-complement-activation-by-inflammatory-stimuli-and-intraocular-inhibition-of-complement-factor-d-in-animal-models
#2
Maura A Crowley, Omar Delgado, Adrian Will-Orrego, Natasha M Buchanan, Karen Anderson, Bruce D Jaffee, Thaddeus P Dryja, Sha-Mei Liao
Purpose: Genome-wide association studies suggest a role for the complement system in age-related macular degeneration (AMD). We characterized ocular complement activation and evaluated a complement factor D (FD) neutralizing antibody. Methods: Mice were treated with toll-like receptor (TLR) ligands, intravitreal injection (IVT), or corneal debridement. Levels of complement proteins and mRNA were measured. A FD neutralizing antibody was administered IVT into eyes of rabbits that were challenged with LPS (lipopolysaccharide) administered intravenously...
February 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29448097/selective-degradation-of-bet-proteins-with-dbet1-a-proteolysis-targeting-chimera-potently-reduces-pro-inflammatory-responses-in-lipopolysaccharide-activated-microglia
#3
Kelly M DeMars, Changjun Yang, Carolina I Castro-Rivera, Eduardo Candelario-Jalil
Bromodomain and extraterminal (BET) proteins are essential to pro-inflammatory gene transcription. The BET family proteins, BRD2, BRD3, BRD4, and testis-specific BRDT, couple chromatin remodeling to gene transcription, acting as histone acetyltransferases, scaffolds for transcription complexes, and markers of histone acetylation. To initiate an inflammatory response, cells undergo de novo gene transcription requiring histone-modifying proteins to make DNA wrapped around histones more or less readily available to transcription complexes...
February 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29444200/loss-of-sirt3-accelerates-arterial-thrombosis-by-increasing-formation-of-neutrophil-extracellular-traps-and-plasma-tissue-factor-activity
#4
Daniel S Gaul, Julien Weber, Lambertus J Van Tits, Susanna Sluka, Lisa Pasterk, Martin F Reiner, Natacha Calatayud, Christine Lohmann, Roland Klingenberg, Jürgen Pahla, Daria Vdovenko, Felix C Tanner, Giovanni G Camici, Urs Eriksson, Johan Auwerx, François Mach, Stephan Windecker, Nicolas Rodondi, Thomas F L Uuml Scher, Stephan Winnik, Christian M Matter
BACKGROUND: Sirtuin 3 (Sirt3) is a mitochondrial, nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase that reduces oxidative stress by activation of superoxide dismutase 2 (SOD2). Oxidative stress enhances arterial thrombosis. This study investigated the effects of genetic Sirt3 deletion on arterial thrombosis in mice in an inflammatory setting and assessed the clinical relevance of these findings in patients with ST-elevation myocardial infarction (STEMI). Methods and Results: Using a laser-induced carotid thrombosis model with lipopolysaccharide (LPS) challenge, in vivo time to thrombotic occlusion in Sirt3-/- mice (n = 6) was reduced by half compared to Sirt3+/+ wildtype (WT, n = 8, p<0...
February 10, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29438224/erlotinib-protects-lps-induced-acute-lung-injury-in-mice-by-inhibiting-egfr-tlr4-signaling-pathway
#5
Huan Tao, Na Li, Zhao Zhang, Honglan Mu, Chen Meng, Huimin Xia, Lisha Fu, Younian Xu, Shihai Zhang
Epidermal growth factor receptor (EGFR) has been reported to initiate the inflammatory response, but its activation in lipopolysaccharide (LPS)-induced murine model of acute lung injury (ALI) remains unclear. In this study, we investigated the role of EGFR in the LPS-induced murine model of ALI and explored whether its inhibitor erlotinib could affect the progression of lung injury. We first detected the phosphorylated EGFR (p-EGFR)/EGFR ratio at different time points after LPS stimulation, and then different concentrations of erlotinib were used to treat mice at 1 h before LPS stimulation and collected samples at the time point of the highest p-EGFR/EGFR ratio...
February 12, 2018: Shock
https://www.readbyqxmd.com/read/29438223/knockdown-of-tfpi-anchored-endothelial-cells-exacerbates-lipopolysaccharide-induced-acute-lung-injury-via-nf-%C3%AE%C2%BAb-signaling-pathway
#6
Bao Q Wang, Meng Shi, Jian P Zhang, Xie Wu, Mei J Chang, Zhi H Chen, Hua H Shen, Yuan L Song, Jian Zhou, Chun X Bai
BACKGROUND: As activation of the coagulation system is both a consequence and contributor to acute lung injury (ALI), pulmonary coagulopathy has become a potential target for therapeutic intervention in ALI patients. We investigated the effects and possible mechanisms of endothelial cell (EC)-anchored tissue factor pathway inhibitor (TFPI) on lipopolysaccharide (LPS)-induced ALI in mice. METHODS: To assess the effect of endothelial cell-anchored TFPI deletion on ALI indices, TFPI knockout (cKO) mice were generated...
February 12, 2018: Shock
https://www.readbyqxmd.com/read/29436098/mitigation-of-inflammatory-immune-responses-with-hydrophilic-nanoparticles
#7
Bowen Li, Shaoyi Jiang, Jingyi Xie, Zhefan Yuan, Priyesh Jain, Xiaojie Lin, Kan Wu
While hydrophobic nanoparticles (NPs) have been long recognized to boost the immune activation, whether hydrophilic NPs modulate an immune system challenged by immune stimulators and how their hydrophilic properties may affect the immune response is still unclear. To answer this question, three polymers, poly(ethylene glycol)(PEG), poly(sulfobetaine)(PSB) and poly(carboxybetaine)(PCB) are studied. For fair comparison, nanogels with uniform size and pure surface functionalities were made from these polymers...
February 12, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29431121/methotrexate-limits-inflammation-through-an-a20-dependent-cross-tolerance-mechanism
#8
Cristina Municio, Ángeles Dominguez-Soto, Sara Fuentelsaz-Romero, Amalia Lamana, Nuria Montes, Víctor D Cuevas, Raquel García Campos, José L Pablos, Isidoro González-Álvaro, Amaya Puig-Kröger
OBJECTIVES: Methotrexate (MTX) is the anchor drug for treatment of rheumatoid arthritis (RA), but the mechanism of its anti-inflammatory action is not fully understood. In RA, macrophages display a proinflammatory polarisation profile that resembles granulocyte-macrophage colony-stimulating factor (GM-CSF)-differentiated macrophages and the response to MTX is only observed in thymidylate synthase+ GM-CSF-dependent macrophages. To determine the molecular basis for the MTX anti-inflammatory action, we explored toll-like receptor (TLR), RA synovial fluid (RASF) and tumour necrosis factor receptor (TNFR)-initiated signalling in MTX-exposed GM-CSF-primed macrophages...
February 3, 2018: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/29421977/chronic-alterations-in-systemic-immune-function-after-traumatic-brain-injury
#9
Rodney Ritzel, Sarah Doran, James Barrett, Rebecca Henry, Elise Ma, Alan Faden, David J Loane
There is a compelling link between severe brain trauma and immunosuppression in traumatic brain injury (TBI) patients. Although acute changes in the systemic immune compartment have been linked to outcome severity, the long-term consequences of TBI on systemic immune function is unknown. Here, adult male C57Bl/6 mice were subjected to moderate-level controlled cortical impact (CCI) or sham surgery and systemic immune function was evaluated at 1, 3, 7, 14, and 60 days post-injury. Bone marrow, blood, thymus, and spleen were examined by flow cytometry to assess changes in immune composition, reactive oxygen species (ROS) production, phagocytic activity, and cytokine production...
February 8, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29419799/inhibition-of-phosphodiesterase-4-by-fcpr03-alleviates-lipopolysaccharide-induced-depressive-like-behaviors-in-mice-involvement-of-p38-and-jnk-signaling-pathways
#10
Hui Yu, Zhengqiang Zou, Xiaolin Zhang, Wanli Peng, Chen Chen, Yicheng Ye, Jiangping Xu, Haitao Wang
Inflammatory responses induced by peripheral administration of lipopolysaccharide (LPS) triggers depressive-like behavioral syndrome in rodents. Inhibition of phosphodiesterase 4 (PDE4) produces a robust anti-inflammatory effect in inflammatory cells. Unfortunately, archetypal PDE4 inhibitors cause intolerable gastrointestinal side-effects, such as vomiting and nausea. N-isopropyl-3-(cyclopropylmethoxy)-4-difluoromethoxy benzamide (FCPR03) is a novel, selective PDE4 inhibitor with little, or no, emetic potency...
February 8, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29412161/comparative-experimental-infection-study-in-mice-with-b-melitensis-and-its-lipopolysaccharide-via-subcutaneous-route-of-inoculation
#11
Abdinasir Yusuf Osman, Abdul Aziz Saharee, Arifah Abdul Kadir
Brucella melitensis is a major zoonotic pathogen in which lipopolysaccharide (LPS) is believed to play a major role in the diseases pathogenesis. To study the immunopathophysiological aspects, we established a mouse model experimentally infected with whole cell of B. melitensis and its lipopolysaccharide via oral route of inoculation. Eighty four mice, BALB/c, both sexes with equal gender distribution and 6-8 weeks-old were randomly assigned into 3 groups. Group 1 (n = 36) were subcutaneoulsy inoculated with 0...
February 2, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29408044/effect-of-lipopolysaccharide-on-the-responsiveness-of-equine-bronchial-tissue
#12
Luigino Calzetta, Paola Rogliani, Elena Pistocchini, Maurizio Mattei, Giuseppe Cito, Pietro Alfonsi, Clive Page, Maria Gabriella Matera
Recurrent airway obstruction (RAO) is a main characteristic of horses with severe equine asthma syndrome. The presence of bacterial lipopolysaccharide (LPS) in the airways of horses is thought to play a crucial role in the clinical expression of this disorder. This study pharmacologically characterized the effect of LPS on the responsiveness of equine bronchial tissue. Equine isolated bronchi were incubated overnight with LPS (0.1-100 ng/ml) and then stimulated by electrical field stimulation (EFS). The role of capsaicin sensitive-sensory nerves (capsaicin desensitization treatment), neurokinin-2 (NK2) receptors (blocked by GR159897), transient receptor potential vanilloid type 1 receptors (TRPV1; blocked by SB366791), and neurokinin A (NKA) were investigated...
January 30, 2018: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29402223/morg1-mice-are-protected-from-histological-renal-damage-and-inflammation-in-a-murine-model-of-endotoxemia
#13
Tzvetanka Bondeva, Claudia Schindler, Katrin Schindler, Gunter Wolf
BACKGROUND: The MAPK-organizer 1 (MORG1) play a scaffold function in the MAPK and/or the PHD3 signalling paths. Recently, we reported that MORG1+/- mice are protected from renal injury induced by systemic hypoxia and acute renal ischemia-reperfusion injury via increased hypoxia-inducible factors (HIFs). Here, we explore whether MORG1 heterozygosity could attenuate renal injury in a murine model of lipopolysaccharide (LPS) induced endotoxemia. METHODS: Endotoxemia was induced in mice by an intraperitoneal (i...
February 5, 2018: BMC Nephrology
https://www.readbyqxmd.com/read/29382344/age-related-cognitive-impairment-is-associated-with-long-term-neuroinflammation-and-oxidative-stress-in-a-mouse-model-of-episodic-systemic-inflammation
#14
Joana Costa d'Avila, Luciana Domett Siqueira, Aurélien Mazeraud, Estefania Pereira Azevedo, Debora Foguel, Hugo Caire Castro-Faria-Neto, Tarek Sharshar, Fabrice Chrétien, Fernando Augusto Bozza
BACKGROUND: Microglia function is essential to maintain the brain homeostasis. Evidence shows that aged microglia are primed and show exaggerated response to acute inflammatory challenge. Systemic inflammation signals to the brain inducing changes that impact cognitive function. However, the mechanisms involved in age-related cognitive decline associated to episodic systemic inflammation are not completely understood. The aim of this study was to identify neuropathological features associated to age-related cognitive decline in a mouse model of episodic systemic inflammation...
January 30, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29367081/immune-modulatory-properties-of-6-gingerol-and-resveratrol-in-atlantic-salmon-macrophages
#15
Nicole C Smith, Sherri L Christian, Richard G Taylor, Javier Santander, Matthew L Rise
The innate immune system provides the first barrier to infection and disease through various processes including activation of inflammation. Inflammation is a biological process whereby immune cells, including macrophages, respond to, and work together to eliminate, damage from injury and disease. Chronic or sustained inflammation, however, can be detrimental to the health and growth of animals, including fish. Immune modulating functional plant compounds, supplemented as feed additives, may be safe and natural approaches to controlling inflammation and disease...
January 19, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29358057/a-bivalent-vaccine-derived-from-attenuated-salmonella-expressing-o-antigen-polysaccharide-provides-protection-against-avian-pathogenic-escherichia-coli-o1-and-o2-infection
#16
Yue Han, Qing Liu, Stephan Willias, Kang Liang, Pei Li, Anchun Cheng, Qingke Kong
Avian pathogenic Escherichia coli (APEC), a leading cause of avian airsacculitis and colibacillosis, is responsible for significant economic loss in the poultry industry. APEC serogroups O1, O2, and O78 are predominantly associated with disease. Lipopolysaccharide (LPS) O-antigen has been shown to be a potent antigen for inducing specific protective immune responses. Therefore, we sought to develop a multivalent polysaccharide vaccine to prevent most APEC infections. We previously reported the stable expression of plasmid pSS27 encoding the APEC O1 O-antigen gene cluster (10...
January 18, 2018: Vaccine
https://www.readbyqxmd.com/read/29353373/disturbance-of-metabotropic-glutamate-receptor-mediated-long-term-depression-mglu-ltd-of-excitatory-synaptic-transmission-in-the-rat-hippocampus-after-prenatal-immune-challenge
#17
Mélanie Cavalier, Azza Ben Sedrine, Lea Thevenet, Nadine Crouzin, Janique Guiramand, Marie-Céleste de Jésus Ferreira, Catherine Cohen-Solal, Gérard Barbanel, Michel Vignes
Maternal immune challenge has proved to induce moderate to severe behavioral disabilities in the offspring. Cognitive/behavioral deficits are supported by changes in synaptic plasticity in different brain areas. We have reported previously that prenatal exposure to bacterial LPS could induce inhibition of hippocampal long-term potentiation (LTP) in the CA1 area of the juvenile/adult male offspring associated with spatial learning inabilities. Nevertheless, deficits in plasticity could be observed at earlier stages as shown by the early loss of long-term depression (LTD) in immature animals...
January 20, 2018: Neurochemical Research
https://www.readbyqxmd.com/read/29343759/incorporation-of-iloprost-in-phospholipase-resistant-phospholipid-scaffold-enhances-its-barrier-protective-effects-on-pulmonary-endothelium
#18
Olga Oskolkova, Nicolene Sarich, Yufeng Tian, Grzegorz Gawlak, Fanyong Meng, Valery N Bochkov, Evgeny Berdyshev, Anna A Birukova, Konstantin G Birukov
Correction of barrier dysfunction and inflammation in acute lung injury (ALI) represents an important problem. Previous studies demonstrate barrier-protective and anti-inflammatory effects of bioactive lipid prostacyclin and its stable analog iloprost (ILO). We generated a phospholipase resistant synthetic phospholipid with iloprost attached at the sn-2 position (ILO-PC) and investigated its biological effects. In comparison to free ILO, ILO-PC caused sustained endothelial cell (EC) barrier enhancement, linked to more prolonged activation of Rap1 and Rac1 GTPases and their cytoskeletal and cell junction effectors: cortactin, PAK1, p120-catenin and VE-cadherin...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29341943/beneficial-effect-of-chronic-treatment-with-extracts-from-rhodiola-rosea-l-and-curcuma-longa-l-on-the-immunoreactivity-of-animals-subjected-to-a-chronic-mild-stress-model
#19
Liliya V Vasileva, Kremena Е Saracheva, Mariya V Ivanovska, Atanaska P Petrova, Emir Sucouglu, Mariana A Murdjeva, Damyanka P Getova-Spasova
BACKGROUND: Recent studies have suggested increased levels of pro-inflammatory cytokines in depression. AIM: The present study aimed to evaluate the effect of extracts from Rhodiola and Curcuma on immunoreactivity of animals subjected to a chronic mild stress (CMS) model followed by lipopolysaccharide-induced inflammation. MATERIALS AND METHODS: Male Wistar rats (n=56) divided in 7 groups were treated orally with: distilled water 10 ml/kg (control and CMS model groups); Rhodiola 250 mg/kg; Rhodiola 500 mg/kg; Curcuma 250 mg/kg; Curcuma 500 mg/kg, Rhodiola 250 mg/kg and Curcuma 250 mg/kg...
December 20, 2017: Folia Medica
https://www.readbyqxmd.com/read/29337327/modulating-inflammation-in-a-cutaneous-chronic-wound-model-by-il-10-released-from-collagen-silica-nanocomposites-via-gene-delivery
#20
Xiaolin Wang, Thibaud Coradin, Christophe Hélary
Cutaneous chronic wounds remain a major clinical challenge which requires the development of novel wound dressings. Previously, we showed that collagen-silica nanocomposites consisting of polyethyleneimine (PEI)-DNA complexes associated with silica nanoparticles (SiNP), collagen hydrogel and 3T3 fibroblasts, can work as a local "cell factory". Indeed, the "in-gel" transfection leads to a sustained production and release of biomolecules. Herein, we further explored the possibility for nanocomposites to deliver interleukin-10 (IL-10), a potent anti-inflammatory cytokine, which favors tissue repair...
January 16, 2018: Biomaterials Science
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