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Donatas Samsanavičius, Vygintas Kaikaris, Simonas-Jonas Norvydas, Rokas Liubauskas, Skaidra Valiukevičienė, Jurgita Makštienė, Kęstutis Maslauskas, Rytis Rimdeika
BACKGROUND AND AIM: Cutaneous squamous cell carcinoma (CSCC) is a malignant epithelial cell tumor. CSCC has a tendency to spread via lymphogenic pathway. Metastases are found in 2%-6% of cases. Prognosis of patients with CSCC is directly related to the morphology and localization of primary tumor. The aim of this study was to evaluate the recurrence-free survival of patients with CSCC after tumor excision and SLNB as well as to analyze morphologic CSCC features related to patient recurrence-free survival...
September 28, 2016: Medicina
Abigail L Lind, Timothy D Smith, Timothy Saterlee, Ana M Calvo, Antonis Rokas
Sensing and responding to environmental cues is critical to the lifestyle of filamentous fungi. How environmental variation influences fungi to produce a wide diversity of ecologically important secondary metabolites (SMs) is not well understood. To address this question, we first examined changes in global gene expression of the opportunistic human pathogen Aspergillus fumigatus after exposure to different temperature conditions. We found that 11 of the 37 SM gene clusters in A. fumigatus were expressed at higher levels at 30°C than at 37°C...
September 30, 2016: G3: Genes—Genomes—Genetics
Xing-Xing Shen, Xiaofan Zhou, Jacek Kominek, Cletus P Kurtzman, Chris Todd Hittinger, Antonis Rokas
Understanding the phylogenetic relationships among the yeasts of the subphylum Saccharomycotina is a prerequisite for understanding the evolution of their metabolisms and ecological lifestyles. In the last two decades, the use of rDNA and multi-locus data sets has greatly advanced our understanding of the yeast phylogeny, but many deep relationships remain unsupported. In contrast, phylogenomic analyses have involved relatively few taxa and lineages that were often selected with limited considerations for covering the breadth of yeast biodiversity...
September 26, 2016: G3: Genes—Genomes—Genetics
Pálma Fehér, Zoltán Ujhelyi, Judit Váradi, Ferenc Fenyvesi, Eszter Róka, Béla Juhász, Balázs Varga, Mariann Bombicz, Dániel Priksz, Ildikó Bácskay, Miklós Vecsernyés
Plants with high amounts of antioxidants may be a promising therapy for preventing and curing UV-induced oxidative skin damage. The objective of this study was to verify the efficacy of topical formulations containing dissolved and suspended Silybum marianum extract against UVB-induced oxidative stress in guinea pig and HaCaT keratinocytes. Herbal extract was dissolved in Transcutol HP (TC) and sucrose-esters were incorporated as penetration enhancers in creams. Biocompatibility of compositions was tested on HeLa cells and HaCaT keratinocytes as in vitro models...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Kirilas Michailovas, Andrius Baltuska, Audrius Pugzlys, Valerijus Smilgevicius, Andrejus Michailovas, Audrius Zaukevicius, Rokas Danilevicius, Saulius Frankinas, Nerijus Rusteika
We report on the developed front-end/pump system for optical parametric chirped pulse amplifiers. The system is based on a dual output fiber oscillator/power amplifier which seeds and assures all-optical synchronization of femtosecond Yb and picosecond Nd laser amplifiers operating at a central wavelength of 1030 nm and 1064 nm, respectively. At the central wavelength of 1030 nm, the fiber oscillator generates partially stretched 4 ps pulses with the spectrum supporting a <120 fs pulse duration and pulse energy of 0...
September 19, 2016: Optics Express
Xiaofan Zhou, David Peris, Jacek Kominek, Cletus P Kurtzman, Chris Todd Hittinger, Antonis Rokas
The availability of genomes across the tree of life is highly biased toward vertebrates, pathogens, human disease models, and organisms with relatively small and simple genomes. Recent progress in genomics has enabled the de novo decoding of the genome of virtually any organism, greatly expanding its potential for understanding the biology and evolution of the full spectrum of biodiversity. The increasing diversity of sequencing technologies, assays, and de novo assembly algorithms have augmented the complexity of de novo genome sequencing projects in non-model organisms...
September 16, 2016: G3: Genes—Genomes—Genetics
Robert Riley, Sajeet Haridas, Kenneth H Wolfe, Mariana R Lopes, Chris Todd Hittinger, Markus Göker, Asaf A Salamov, Jennifer H Wisecaver, Tanya M Long, Christopher H Calvey, Andrea L Aerts, Kerrie W Barry, Cindy Choi, Alicia Clum, Aisling Y Coughlan, Shweta Deshpande, Alexander P Douglass, Sara J Hanson, Hans-Peter Klenk, Kurt M LaButti, Alla Lapidus, Erika A Lindquist, Anna M Lipzen, Jan P Meier-Kolthoff, Robin A Ohm, Robert P Otillar, Jasmyn L Pangilinan, Yi Peng, Antonis Rokas, Carlos A Rosa, Carmen Scheuner, Andriy A Sibirny, Jason C Slot, J Benjamin Stielow, Hui Sun, Cletus P Kurtzman, Meredith Blackwell, Igor V Grigoriev, Thomas W Jeffries
Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as l-rhamnose utilization, have patchy phylogenetic distributions...
August 30, 2016: Proceedings of the National Academy of Sciences of the United States of America
Onesmus Maina Muchemi, Agnes Wangechi Gichogo, Jane Githuku Mungai, Zeinab Gura Roka
INTRODUCTION: WHO classifies Kenya as having a high maternal mortality. Regional data on maternal mortality trends is only available in selected areas. This study reviewed health facility maternal mortality trends, causes and distribution in Central Region of Kenya, 2008-2012. METHODS: We reviewed health records from July 2008 to June 2012. A maternal death was defined according to ICD-10 criterion. The variables reviewed included socio-demographic, obstetric characteristics, reasons for admission, causes of death and contributing factors...
2016: Pan African Medical Journal
Xing-Xing Shen, Leonidas Salichos, Antonis Rokas
Molecular phylogenetic inference is inherently dependent on choices in both methodology and data. Many insightful studies have shown how choices in methodology, such as the model of sequence evolution or optimality criterion used, can strongly influence inference. In contrast, much less is known about the impact of choices in the properties of the data, typically genes, on phylogenetic inference. We investigated the relationships between 52 gene properties (24 sequence-based, 19 function-based, and 9 tree-based) with each other and with three measures of phylogenetic signal in two assembled data sets of 2,832 yeast and 2,002 mammalian genes...
2016: Genome Biology and Evolution
William E Ackerman, Irina A Buhimschi, Haley R Eidem, David C Rinker, Antonis Rokas, Kara Rood, Guomao Zhao, Taryn L Summerfield, Mark B Landon, Catalin S Buhimschi
INTRODUCTION: We performed RNA sequencing with the primary goal of discovering key placental villous trophoblast (VT) and decidua basalis (DB) transcripts differentially expressed in intra-amniotic infection (IAI)-induced preterm birth (PTB). METHODS: RNA was extracted from 15 paired VT and DB specimens delivered of women with: 1) spontaneous PTB in the setting of amniocentesis-proven IAI and histological chorioamnionitis (n = 5); 2) spontaneous idiopathic PTB (iPTB, n = 5); and 3) physiologic term pregnancy (n = 5)...
August 2016: Placenta
Ramunas Grigonis, Robertas Guzulaitis, Rokas Buisas, Aidas Alaburda
During functional spinal neural network activity motoneurons receive massive synaptic excitation and inhibition, and their membrane conductance increases considerably - they are switched to a high-conductance state. High-conductance states can substantially alter response properties of motoneurons. In the present study we investigated how an increase in membrane conductance affects spike frequency adaptation, the gain (i.e., the slope of the frequency-current relationship) and the threshold for action potential generation...
October 1, 2016: Brain Research
Rokas Navickas, Diane Gal, Aleksandras Laucevičius, Agnė Taparauskaitė, Monika Zdanytė, Paul Holvoet
The aim of the present study is to identify microRNAs (miRs) with high potential to be used as biomarkers in plasma and/or serum to clinically diagnose, or provide accurate prognosis for survival in, patients with atherosclerosis, coronary artery disease, and acute coronary syndrome (ACS). A systematic search of published original research yielded a total of 72 studies. After review of the risk of bias of the published studies, according to Cochrane Collaboration and the QUADUAS Group standards, 19 studies were selected...
September 2016: Cardiovascular Research
Johannes Steinbacher, Ines E Tinhofer, Stefan Meng, Lukas F Reissig, Eva Placheta, Julia Roka-Palkovits, Thomas Rath, Ming-Huei Cheng, Wolfgang J Weninger, Chieh-Han J Tzou
BACKGROUND: Vascularized lymph node transfer is an effective surgical method in reducing lymphedema. This study provides the first detailed description of the surgical anatomy of the supraclavicular lymph node flap in regard to pedicle length, pedicle diameter, and the number of lymph nodes and their exact location inside the flap. METHODS: Bilateral supraclavicular dissections of nine fresh cadavers (five female) were performed. Before the dissection, the exact number of lymph nodes was determined sonographically by an experienced radiologist, and their distance from the jugular notch was measured...
June 28, 2016: Journal of Surgical Oncology
Chieh-Han J Tzou, Stefan Meng, Tinhofer Ines, Lukas Reissig, Ursula Pichler, Johannes Steinbacher, Igor Pona, Julia Roka-Palkovits, Thomas Rath, Wolfgang J Weninger, Ming-Huei Cheng
BACKGROUND: Harvesting the submental flap for vascularized lymph node transfer (VLNT) presents a challenging procedure because of, the topographic variation of the submental artery (SA) and the marginal mandible nerve (MMN) and the limited pedicle length for a free tissue transfer. The aim of this study was to evaluate surgical anatomical landmarks and variations of the submental lymph node flap (SLNF). METHODS AND MATERIALS: The authors examined the characteristics and landmarks of 18 SLNF in nine fresh cadavers...
June 23, 2016: Journal of Surgical Oncology
A D Waldmann, P L Róka, S H Bohm, W Windisch, S Strassmann, C Karagiannidis
No abstract text is available yet for this article.
December 2015: Intensive Care Medicine Experimental
P L Róka, A D Waldmann, F Ender, S H Bohm, W Windisch, S Strassmann, C Karagiannidis
No abstract text is available yet for this article.
December 2015: Intensive Care Medicine Experimental
Haley R Eidem, David C Rinker, William E Ackerman, Irina A Buhimschi, Catalin S Buhimschi, Caitlin Dunn-Fletcher, Suhas G Kallapur, Mihaela Pavličev, Louis J Muglia, Patrick Abbot, Antonis Rokas
INTRODUCTION: A major issue in the transcriptomic study of spontaneous preterm birth (sPTB) in humans is the inability to collect healthy control tissue at the same gestational age (GA) to compare with pathologic preterm tissue. Thus, gene expression differences identified after the standard comparison of sPTB and term tissues necessarily reflect differences in both sPTB pathology and GA. One potential solution is to use GA-matched controls from a closely related species to tease apart genes that are dysregulated during sPTB from genes that are expressed differently as a result of GA effects...
May 2016: Placenta
Dorottya Laczkó, András Rosztóczy, Klaudia Birkás, Máté Katona, Zoltán Rakonczay, László Tiszlavicz, Richárd Róka, Tibor Wittmann, Péter Hegyi, Viktória Venglovecz
Barrett's esophagus (BE) is considered to be the most severe complication of gastro-esophageal reflux disease (GERD), in which the prolonged, repetitive episodes of combined acidic and biliary reflux result in the replacement of the squamous esophageal lining by columnar epithelium. Therefore, the acid-extruding mechanisms of esophageal epithelial cells (EECs) may play an important role in the defense. Our aim was to identify the presence of acid/base transporters on EECs and to investigate the effect of bile acids on their expressions and functions...
July 1, 2016: American Journal of Physiology. Gastrointestinal and Liver Physiology
Jennifer H Wisecaver, William G Alexander, Sean B King, Chris Todd Hittinger, Antonis Rokas
Due to their functional independence, proteins that comprise standalone metabolic units, which we name single-protein metabolic modules, may be particularly prone to gene duplication (GD) and horizontal gene transfer (HGT). Flavohemoglobins (flavoHbs) are prime examples of single-protein metabolic modules, detoxifying nitric oxide (NO), a ubiquitous toxin whose antimicrobial properties many life forms exploit, to nitrate, a common source of nitrogen for organisms. FlavoHbs appear widespread in bacteria and have been identified in a handful of microbial eukaryotes, but how the distribution of this ecologically and biomedically important protein family evolved remains unknown...
August 2016: Molecular Biology and Evolution
Kyu Sik Chang, Dae-Hyun Yoo, Young Ran Ju, Wook Gyo Lee, Jong Yul Roh, Heung-Chul Kim, Terry A Klein, E-Hyun Shin
BACKGROUND: As a result of the reintroduction of malaria in the Republic of Korea (ROK) in 1993 and the threat to military and civilian populations, the Korea Military National Defense (MND) increased emphasis on vector control in 2012 at ROK Army (ROKA) installations located near the DMZ, while decreasing chemoprophylaxis, fearing potential drug resistance. Mosquito surveillance demonstrated a need for continuous monitoring of disease patterns among ROKA soldiers and vector malaria infection rates to ensure positive outcomes...
2016: Malaria Journal
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