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https://www.readbyqxmd.com/read/28738348/colistin-resistance-in-kpc-2-and-shv-5-producing-klebsiella-pneumoniae-clinical-isolates-in-bulgaria
#1
Encho Savov, Iva Todorova, Lida Politi, Angelina Trifonova, Maja Borisova, Elena Kioseva, Athanassios Tsakris
BACKGROUND/AIMS: Colistin resistance is increasingly recognized among carbapenemase-producing Klebsiella pneumoniae isolates in several European regions. The current study documents the appearance of colistin resistance among KPC-2 and SHV-5-produning K. pneumoniae strains in Bulgaria. METHODS: Four colistin-resistant K. pneumoniae isolates were recovered from 2 patients hospitalized in the anesthesiology and resuscitation clinic of a tertiary care university hospital in Sofia, Bulgaria...
July 22, 2017: Chemotherapy
https://www.readbyqxmd.com/read/28704202/the-integration-of-the-molecular-methods-in-the-diagnosis-algorithm-for-the-poliovirus-detection-in-the-sewage-water-comparing-concentration-and-detection-methods-a-pilot-study
#2
Anda Bãicuş
Introduction Two cases of circulating vaccine-derived poliovirus type 1(cVDPV1), from southwestern Ukraine, bordering Romania, were confirmed in 2015 and the environmental enterovirus surveillance was enhanced in our country. The molecular detection of human enteroviruses as a screening test followed by isolation on cell culture lines or sequencing could be proposed as a new diagnosis algorithm. Material and Methods The sensitivity of two molecular methods for the detection of enterovirus strains in 10 ml of sewage water (15 samples) was studied with Film Array ME panel BioFire (Biomerieux, France) and Xpert EV assay (Cepheid, USA)...
July 8, 2017: Romanian Journal of Internal Medicine, Revue Roumaine de Médecine Interne
https://www.readbyqxmd.com/read/28703892/disinfection-of-the-peritoneal-dialysis-bag-medication-port-comparison-of-disinfectant-agent-and-disinfection-time
#3
Adriana Conti, Roberta Monteiro Katzap, Carlos Eduardo Poli-de-Figueiredo, Vany Pagnussatti, Ana Elizabeth Figueiredo
OBJECTIVE: To compare different disinfection techniques for the peritoneal dialysis bag medication port (MP). METHODS: An experimental study was conducted testing different cleaning agents (70% alcohol vs 2% chlorhexidine) and time periods (5, 10 and 60 seconds) for disinfection of the MP. Five microorganisms (S. aureus, E.coli, A. baumannii and C. parapsilosis, CNS) were prepared for use as contaminants of the MP. MP were incubated in Tryptic soy broth at 36 °C for 24 h, after which, they were seeded on a Biomérieux® blood agar plate and incubated for 24 h at 36 °C...
July 13, 2017: Nephrology
https://www.readbyqxmd.com/read/28702079/rare-bacterial-isolates-causing-bloodstream-infections-in-ethiopian-patients-with-cancer
#4
Balew Arega, Yimtubezinash Wolde-Amanuel, Kelemework Adane, Ezra Belay, Abdulaziz Abubeker, Daniel Asrat
BACKGROUND: In recent years, saprophytic bacteria have been emerging as potential human pathogens causing life-threatening infections in patients with malignancies. However, evidence is lacking concerning such bacteria, particularly in sub-Saharan countries. This study was designed to determine the spectrum and drug resistance profile of the rare bacterial pathogens causing bloodstream infections (BSIs) in febrile cancer patients at a referral hospital in Ethiopia. METHODS: Between December 2011 and June 2012, blood samples were collected from 107 patients with cancer in Tikur Anbessa hospital...
2017: Infectious Agents and Cancer
https://www.readbyqxmd.com/read/28690735/-malaria-serology-test-what-contribution-does-it-make-in-an-endemic-country-such-as-ivory-coast
#5
Amah Patricia Victorine Goran-Kouacou, Gonat Serge Dou, Kalou Dibert Zika, Adjoumanvoulé Honoré Adou, Oppong Richard Yéboah, Rita Ahou Aka, Sansan Hien, Kouabla Liliane Siransy, Koffi N'Guessan, Tariam Agnès Djibangar, Séry Romuald Dassé, Koffi Daho Adoubryn
INTRODUCTION: Malaria serology test seems to have attracted very little interest in endemic countries such as Ivory Coast. However, this examination has been regularly performed in the parasitology laboratory at the Training and Research Unit of Medical Sciences in Abidjan. Our study aimed to highlight the contribution of malaria serology test in our endemic country context. METHODS: We conducted a retrospective study of malaria serology test using Falciparum-Spot IF (bioMerieux) kit for the detection of IgG antiplasmodial antibodies...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28685188/evaluation-of-mycotube-a-modified-version-of-lowenstein-jensen-lj-medium-for-efficient-recovery-of-mycobacterium-tuberculosis-mtb
#6
R Nambiar, S Chatellier, N Bereksi, A van Belkum, N Singh, B Barua, A Shetty, C Rodrigues
Timely diagnosis of tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), is only achieved for ~58% cases. An improved, accurate, time- and cost-effective method for bacteriological confirmation of MTB is necessary. We evaluated Mycotube, a new variant of Lowenstein-Jensen (LJ) culture medium, by comparing it with Mycobacterium Growth Indicator Tube (MGIT) 960 (gold standard), local LJ, and bioMérieux LJ-T in terms of isolation rate and time-to-growth. Pulmonary and extra-pulmonary samples from treatment-naïve suspects (n = 207) were decontaminated by the N-acetyl-L-cysteine-sodium hydroxide method and used to inoculate the four media...
July 6, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28674064/oxa-244-producing-escherichia-coli-isolates-a-challenge-for-clinical-microbiology-laboratories
#7
Yannick Hoyos-Mallecot, Thierry Naas, Rémy A Bonnin, Rafael Patino, Philippe Glaser, Nicolas Fortineau, Laurent Dortet
OXA-244 is a single point mutant derivative of OXA-48 displaying reduced carbapenemase activity. Here, we report microbiological features of 7 OXA-244-producing E. coli isolates. Only one isolate grew on the ChromID® CARBA SMART medium (bioMérieux), but 6/7 grew on ChromID® ESBL medium (bioMérieux), as they co-produced either an extended spectrum β-lactamase and or a plasmid-encoded cephalosporinase. The production of a carbapenemase was detected in 57.1%, 71.4%, 71.4%, and 100% of the E. coli isolates using the Carba NP test, the RAPIDEC® CARBA NP (bioMérieux), a MALDI-TOF MS hydrolysis assay (Bruker), and OXA-48 K-SeT® (Coris bioconcept), respectively...
July 3, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28658756/unusual-severe-extra-intestinal-manifestations-of-a-common-enteric-pathogen-aeromonas-spp
#8
Barnini Banerjee, Mridula Madiyal, Lingadakai Ramchandra, Chiranjay Mukhopadhyay, Rahul Garg, Kiran Chawla
INTRODUCTION: Aeromonas spp. are halophilic, Gram-negative bacilli. They are widely distributed in the soil and aquatic environment and have been associated with various extra-intestinal infections, such as skin and soft-tissue infections, meningitis, bacteraemia etc. The most common species associated with extra-intestinal infection is Aeromonas hydrophila. AIM: To get the overview of clinical presentations, underlying predisposing factors associated with the extra-intestinal infections caused by Aeromonas species and their antimicrobial susceptibility pattern...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28647397/actinomyces-radicidentis-and-actinomyces-haliotis-coccoid-actinomyces-species-isolated-from-the-human-oral-cavity
#9
Rolf Claesson, Ulf Sjögren, Anders Esberg, Malin Brundin, Margareta Granlund
There are few reports on the bacterial species Actinomyces radicidentis in the literature. In this study, putative A. radicidentis isolates were collected from 16 root canal samples from 601 examined patients. The isolates were examined by biochemical tests, 16S rRNA gene sequencing, Arbitrarily-primed (AP-) PCR, antibiotic susceptibility testing, and MALDI-TOF analyses. In parallel, two A. radicidentis reference strains and two putative A. radicidentis isolates from United Kingdom were tested. Sixteen of the 18 isolates were confirmed as A...
June 21, 2017: Anaerobe
https://www.readbyqxmd.com/read/28643493/identification-of-viridans-streptococci-with-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-by-an-in-house-method-and-a-commercially-available-system
#10
Catalina Suzana Stingu, Klaus Eschrich, Juliane Thiel, Toralf Borgmann, Reiner Schaumann, Arne C Rodloff
Two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)-based methods were compared for their ability to identify viridans streptococci. One approach employed a reference database and software developed in-house. All inhouse measurements were performed using an Autoflex II Instrument (Bruker Daltonics GmbH, Germany). The other system, a VITEK-MS (BioMérieux, France) was operated on the commercially available V2.0 Knowledge Base for Clinical Use database. Clinical isolates of viridans streptococci (n=184) were examined...
September 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28632835/-evaluation-of-antimicrobial-susceptibility-testing-directly-from-positive-blood-cultures-using-the-vitek-2-system-comparison-of-two-rapid-protocols
#11
Lorena Porte, Francesca Gattini, Carmen Varela, Thomas Weitzel
Antimicrobial susceptibility testing using Vitek® 2 (bioMérieux) cards inoculated directly from positive blood cultures was compared with the standard method for gramnegative rods. Two different protocols for the inoculum preparation were evaluated. Protocol 2 was faster and easier to perform. Rapid susceptibility testing was accurate and reduced time to results in 24 h.
April 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28611406/targeted-next-generation-sequencing-of-the-16s-23s-rrna-region-for-culture-independent-bacterial-identification-increased-discrimination-of-closely-related-species
#12
Artur J Sabat, Evert van Zanten, Viktoria Akkerboom, Guido Wisselink, Kees van Slochteren, Richard F de Boer, Ron Hendrix, Alexander W Friedrich, John W A Rossen, Anna M D Mirjam Kooistra-Smid
The aim of this study was to develop an easy-to-use culture-free diagnostic method based on next generation sequencing (NGS) of PCR amplification products encompassing whole 16S-23S rRNA region to improve the resolution of bacterial species identification. To determine the resolution of the new method 67 isolates were subjected to four identification methods: Sanger sequencing of the 16S rRNA gene; NGS of the 16S-23S rRNA region using MiSeq (Illumina) sequencer; Microflex MS (Bruker) and VITEK MS (bioMérieux)...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28606185/non-malarial-infectious-diseases-of-antenatal-care-in-pregnant-women-in-franceville-gabon
#13
Irene Pegha Moukandja, Edgard Brice Ngoungou, Guy Joseph Lemamy, Ulrick Bisvigou, Antoine Gessain, Fousseyni S Toure Ndouo, Mirdad Kazanji, Jean Bernard Lekana-Douki
BACKGROUND: In sub-tropical countries, infectious diseases remain one of the main causes of mortality. Because of their lack of active immunity, pregnant women and their unborn children represent the most susceptible people. In Gabon, data on infectious diseases of pregnant women such as syphilis and rubella are either scarce or very old. Few studies have assessed T. gondii infection during pregnancy in the country. Here, we evaluate seroprevalence of HIV, HTVL-1, syphilis and T. gondii and rubella infection during antenatal care among women living in Franceville, Gabon...
June 12, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/28596960/acinetobacter-baumannii-infection-in-transfusion-dependent-thalassemia-patients-with-sepsis
#14
Muzaheed Abdul Rasheed, Faisal Mousa Alzahrani, Saeed Sattar Shaikh
PURPOSE: To identify the Acinetobacter baumannii infection among transfusion dependent thalassemia patients. METHODS: A quantitative approach was employed to assess Acinetobacter baumannii infection in transfusion dependent thalassemia patients. Samples were collected from 916 patients, which have shown bacterial growth on MacConkey and blood agar culture media. A. baumannii strains were identified by microbiological methods and Gram's staining. API 20 E kit (Biomerieux, USA) was used for final identification...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28595598/isolation-biochemical-and-molecular-identification-of-nocardia-species-among-tb-suspects-in-northeastern-tanzania-a-forgotten-or-neglected-threat
#15
Abubakar S Hoza, Sayoki G S Mfinanga, Irmgard Moser, Brigitte König
BACKGROUND: Pulmonary nocardiosis mimic pulmonary tuberculosis in most clinical and radiological manifestations. In Tanzania, where tuberculosis is one of the major public health threat clinical impact of nocardiosis as the cause of the human disease remains unknown. The objective of the present study was to isolate and identify Nocardia isolates recovered from TB suspects in Northeastern, Tanzania by using biochemical and molecular methods. METHODS: The study involved 744 sputum samples collected from 372 TB suspects from four periphery diagnostic centers in Northeastern, Tanzania...
June 8, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28591972/-one-carrier-of-robinsoniella-peoriensis-in-china
#16
Jing-Yu Yang, Xiao-Xia Zhang, Ru-Jia Yu, Ping Feng, Zhi-Yong Zong
OBJECTIVES: To study morphological feature, biochemical characteristic and antibiotic resistance of Robinsoniella peoriensis (R.peoriensis) strain. METHODS: The clinical R.peoriensis strain was isolated from ananus swab samples being screened in ICU. Biochemical characteristic of the strain was completed by fully automatic microbial identification and drug susceptibility analysis system (BioMérieux, Marcy-l'éE toile, France) with the ANC Vitek2. Antibiotic susceptibility in vitro was performed using agar dilution...
July 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28581267/characterization-of-oropharyngeal-carriage-isolates-of-neisseria-meningitidis-in-healthy-korean-adolescents-in-2015
#17
Han Wool Kim, Soyoung Lee, Daeho Kwon, Jihei Cha, Jong Gyun Ahn, Kyung Hyo Kim
The meningococcus carriage rate is age-dependent, with a high prevalence in adolescents and young adults. This cross-sectional study aimed to estimate the oropharyngeal carriage rate of meningococcus among healthy Korean adolescents and its relationship with several population characteristics. The survey was conducted from April to May 2015 among 1,460 first-year high-school students in 9 high schools located in Gyeonggi province, Korea. Each student answered a short questionnaire assessing risk factors for carriage, and posterior pharyngeal wall swab samples were obtained...
July 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28566082/-femoral-hemodialysis-catheter-related-bacteremia-due-to-globicatella-sanguinis-challenges-in-species-identification
#18
Elif Aktaş, Nafia Canan Gürsoy, Tamer Sakacı, Yener Koç, Aziz Ahmad Hamidi, Emin Bulut, Duygu Erdemir, Barış Otlu
In this case, catheter-related bacteremia due to Globicatella sanguinis in a 43 years old female patient undergoing hemodialysis with the diagnosis of diabetic nephropathy was presented and the methods in the laboratory diagnosis of the rare opportunistic pathogen, Globicatella cins, were nvestigated. A set of peripheral blood cultures and simultaneous catheter culture was obtained from the patient in third of May 2016. Biochemical tests, Phoenix (Becton Dickinson, USA) and MicroScan (Beckman Coulter, USA) automated systems and matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) based Microflex MS (Bruker, Daltonics, Germany) and VITEK MS (database v2...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28566081/-a-rarely-isolated-bacterium-in-microbiology-laboratories-streptococcus-uberis
#19
Canan Eryıldız, Şebnem Bukavaz, Şaban Gürcan, Osman Hatipoğlu
Streptococcus uberis is a gram-positive bacterium that is mostly responsible for mastitis in cattle. The bacterium rarely has been associated with human infections. Conventional phenotyphic methods can be inadequate for the identification of S.uberis; and in microbiology laboratories S.uberis is confused with the other streptococci and enterococci isolates. Recently, molecular methods are recommended for the accurate identification of S.uberis isolates. The aim of this report is to present a lower respiratory tract infection case caused by S...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28552659/antimicrobial-activity-evaluation-and-comparison-of-methods-of-susceptibility-for-klebsiella-pneumoniae-carbapenemase-kpc-producing-enterobacter-spp-isolates
#20
Daniele Zendrini Rechenchoski, Angélica Marim Lopes Dambrozio, Ana Carolina Polano Vivan, Paulo Alfonso Schuroff, Tatiane das Neves Burgos, Marsileni Pelisson, Marcia Regina Eches Perugini, Eliana Carolina Vespero
The production of KPC (Klebsiella pneumoniae carbapenemase) is the major mechanism of resistance to carbapenem agents in enterobacterias. In this context, forty KPC-producing Enterobacter spp. clinical isolates were studied. It was evaluated the activity of antimicrobial agents: polymyxin B, tigecycline, ertapenem, imipenem and meropenem, and was performed a comparison of the methodologies used to determine the susceptibility: broth microdilution, Etest(®) (bioMérieux), Vitek 2(®) automated system (bioMérieux) and disc diffusion...
July 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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