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https://www.readbyqxmd.com/read/29769277/rapid-screening-of-urinary-tract-infection-and-discrimination-of-gram-positive-and-gram-negative-bacteria-by-automated-flow-cytometric-analysis-using-sysmex-uf-5000
#1
Seon Young Kim, Yumi Park, Hyunjin Kim, Jimyung Kim, Sun Hoe Koo, Gye Cheol Kwon
Rapid screening of urinary tract infection is important to determine antibiotic treatment and reduce unnecessary urine culture. We evaluated the performance of the new flow cytometry-based UF-5000 automated urine analyzer (Sysmex, Kobe, Japan). A total of 1,430 urine samples from 1,226 patients were analyzed and compared with urine cultures to which a Previ Isola (bioMérieux, Marcy l'Etoile, France) system was applied. In total, 878 of 1,430 urine cultures (61.4%) produced ≥ 103 CFU/mL bacterial growth [lsqb]309 with Gram-negative (GN) bacteria, 517 with Gram-positive (GP) bacteria, and 52 mixed cultures[rsqb], with 336 samples (23...
May 16, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29709574/detection-of-bla-oxa-48-and-clonal-relationship-in-carbapenem-resistant-k-pneumoniae-isolates-at-a-tertiary-care-center-in-western-turkey
#2
Gulfem Ece, Emine Tunc, Baris Otlu, Deniz Aslan, Cem Ece
BACKGROUND: Klebsiella pneumoniae is an important nosocomial pathogen that can lead to high morbidity and mortality. ESBL and carbapenamase producing strains may cause epidemic situations. The aim of our study was to investigate the molecular epidemiology and clonal relationship between carbapenem resistant K. pneumoniae strains isolated from our hospital during a three month period. METHODS: Fourteen carbapenem resistant K. pneumoniae strains isolated during April 1st-June 30th 2013 were included...
April 27, 2018: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/29707661/vim-klebsiella-oxytoca-outbreak-in-a-neonatal-intensive-care-unit-this-time-it-wasn-t-the-drain
#3
R Herruzo, G Ruiz, S Gallego, J Diez, A Sarria, F Omeñaca
Objective: We describe an outbreak of VIM-carbapenemase Klebsiella oxytoca (VIM-Kox) in a NICU. Materials and methods: Prospective Epidemiological Surveillance:Systematically (weekly screening cultures) or on admission, if the patient had a history of previous colonization by VIM-Kox.Clinical cultures, done if infection was suspected.Other possible microorganism sources were investigated: their mothers (rectal microbiota), milk packages and preparation apparata in the lactodietary section, echocardiagram transductors, cribs, the sinks (faucets and drains), washing bowls, etc...
December 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/29698665/high-prevalence-of-multidrug-resistant-international-clones-among-macrolide-resistant-streptococcus-pneumoniae-strains-in-immunocompromised-patients-in-tunisia
#4
Anis Raddaoui, Farah Ben Tanfous, Yosra Chebbi, Wafa Achour, Rekaya Baaboura, Assia Benhassen
Macrolide resistant Streptococcus pneumoniae (S.pneumoniae) isolates increased considerably the last decade with important geographical variations among involved phenotypes and genotypes. The aim of this study was to investigate phenotypes, genotypes, serotypes and genetic relatedness of macrolide resistant S. pneumoniae strains isolated from immunocompromised Patients Antibiotic susceptibility was studied by disk diffusion method and minimum inhibitory concentrations (MIC) of erythromycin and clindamycin were determined by Etest (®Biomerieux)...
April 23, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29690890/a-novel-field-based-molecular-assay-to-detect-validated-artemisinin-resistant-k13-mutants
#5
Laurence Vachot-Ganée, Nimol Khim, Alexandra Iannello, Eric Legrand, Saorin Kim, Rotha Eam, Chanra Khean, Malen Ken, Elizabeth Ashley, Kyaw Myo Tun, Mehul Dhorda, François Nosten, Issa Mahamat Souleymane, Sophie Blein, Alexandre Pachot, Frédéric Ariey, Karine Kaiser, Didier Ménard
BACKGROUND: Given the risk of artemisinin resistance spreading from the Greater Mekong sub-region, prospective monitoring in sub-Saharan Africa should be expedited. Molecular biology techniques used for monitoring rely on the detection of k13 validated mutants by using PCR and Sanger sequencing approach, usually not available in malaria endemic areas. METHODS: A semi-automated workflow based on the easyMAG® platform and the Argene Solution® (bioMérieux, Marcy l'Etoile, France) as a field-based surveillance tool operable at national level was developed in four steps...
April 24, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29681513/antimicrobial-consumption-and-resistance-in-adult-hospital-inpatients-in-53-countries-results-of-an-internet-based-global-point-prevalence-survey
#6
Ann Versporten, Peter Zarb, Isabelle Caniaux, Marie-Françoise Gros, Nico Drapier, Mark Miller, Vincent Jarlier, Dilip Nathwani, Herman Goossens
BACKGROUND: The Global Point Prevalence Survey (Global-PPS) established an international network of hospitals to measure antimicrobial prescribing and resistance worldwide. We aimed to assess antimicrobial prescribing and resistance in hospital inpatients. METHODS: We used a standardised surveillance method to collect detailed data about antimicrobial prescribing and resistance from hospitals worldwide, which were grouped by UN region. The internet-based survey included all inpatients (adults, children, and neonates) receiving an antimicrobial who were on the ward at 0800 h on one specific day between January and September, 2015...
April 19, 2018: Lancet Global Health
https://www.readbyqxmd.com/read/29678480/gram-negative-anaerobe-susceptibility-testing-in-clinical-isolates-using-sensititre-and-etest-methods
#7
C Hughes, C Ashhurst-Smith, J K Ferguson
Gram negative anaerobic organisms are important pathogens in a range of clinical infections, and susceptibility testing is not commonly performed in the microbiology laboratory. We performed anaerobic susceptibility testing on 70 clinically relevant Gram negative anaerobes isolated from routine cultures in a busy diagnostic laboratory which were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The susceptibility testing was performed by two methods: Sensititre trays (ThermoFisher Scientific) against 15 different antibiotics, and Etests (bioMérieux) against five clinically relevant antibiotics (metronidazole, piperacillin-tazobactam, amoxicillin-clavulanate, meropenem and clindamycin)...
April 17, 2018: Pathology
https://www.readbyqxmd.com/read/29656457/late-sampling-for-automated-culture-to-extend-the-platelet-shelf-life-to-5-days-in-germany
#8
Tanja Vollmer, Mareike Dabisch-Ruthe, Melanie Weinstock, Cornelius Knabbe, Jens Dreier
BACKGROUND: Bacterial contamination of platelet concentrates (PCs) is still a major challenge in transfusion medicine. Different methodologic concepts and screening strategies have been developed and investigated concerning their usability. We evaluated the feasibility of BacT/ALERT automated culture (BacT/A, bioMérieux) with late sampling after 3 days at the earliest. STUDY DESIGN AND METHODS: Twenty-four bacterial strains isolated from PCs and six relevant strains from reference stocks were spiked into apheresis-derived PCs (10-60 colony-forming units [CFU]/bag)...
April 15, 2018: Transfusion
https://www.readbyqxmd.com/read/29656124/development-of-an-improved-rapid-bacpro%C3%A2-protocol-and-a-method-for-direct-identification-from-blood-culture-positive-bottles-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#9
Takatoshi Yonezawa, Tomohisa Watari, Kazuho Ashizawa, Daisuke Hanada, Takako Yanagiya, Naoki Watanabe, Takashi Terada, Yutaka Tomoda, Satoshi Fujii
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been incorporated into pathogenic bacterial identification methods and has improved their rapidity. Various methods have been reported to directly identify bacteria with MALDI-TOF MS by pretreating culture medium in blood culture bottles. Rapid BACpro® (Nittobo Medical Co., Ltd.) is a pretreatment kit for effective collection of bacteria with cationic copolymers. However, the Rapid BACpro® pretreatment kit is adapted only for MALDI Biotyper (Bruker Daltonics K...
April 12, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29642831/-identification-of-anaerobic-gram-negative-bacilli-isolated-from-various-clinical-specimens-and-determination-of-antibiotic-resistance-profiles-with-e-test-methods
#10
Cengiz Demir, Recep Keşli
The aim of this study was to identify gram-negative anaerobic bacilli isolated from various clinical specimens that were obtained from patients with suspected anaerobic infections and to determine the antibiotic resistance profiles by using the antibiotic concentration gradient method. The study was performed in Afyon Kocatepe University Ahmet Necdet Sezer Research and Practice Hospital, Medical Microbiology Laboratory between 1 November 2014 and 30 October 2015. Two hundred and seventyeight clinical specimens accepted for anaerobic culture were enrolled in the study...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29628352/-identification-of-yeasts-from-commercial-atti%C3%A3-k%C3%A3-in-abidjan-c%C3%A3-te-d-ivoire-preliminary-study
#11
C G Kouadio-Yapo, G S P Dou, N A D Aka, K D Zika, K D Adoubryn, M Dosso
OBJECTIVE: This study aims to identify yeasts from commercial attiéké. PATIENTS AND METHODS: This descriptive cross-sectional survey involving 103 samples of attiéké sold on the market from August to September 2017. It was conducted from the mycology laboratory of the Institut Pasteur of Côte d'Ivoire. The samples were seeded on YGC medium. The colonies of positive culture were counted and seeded again on chromogen medium. Identification of yeasts species was carried out by MALDI-TOF mass spectrometry (Vitek MS bioMérieux)...
April 6, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29607515/implementation-of-secondary-bacterial-culture-testing-of-platelets-to-mitigate-residual-risk-of-septic-transfusion-reactions
#12
Evan M Bloch, Christi E Marshall, Joan S Boyd, Lisa Shifflett, Aaron A R Tobian, Eric A Gehrie, Paul M Ness
BACKGROUND: Bacterial contamination of platelets remains a major transfusion-associated risk despite long-standing safety measures in the United States. We evaluated an approach using secondary bacterial culture (SBC) to contend with residual risk of bacterial contamination. STUDY DESIGN AND METHODS: Phased implementation of SBC was initiated in October 2016 for platelets (all apheresis collected) received at our institution from the blood donor center (Day 3 post collection)...
April 1, 2018: Transfusion
https://www.readbyqxmd.com/read/29594800/diagnostic-accuracy-of-the-inbios-amd-rapid-diagnostic-test-for-the-detection-of-burkholderia-pseudomallei-antigen-in-grown-blood-culture-broth
#13
Marjan Peeters, Panha Chung, Hua Lin, Kristien Mortelmans, Chhundy Phe, Chentha San, Laura Maria Francisca Kuijpers, Syna Teav, Thong Phe, Jan Jacobs
To assess the diagnostic and operational performance of the InBiOS AMD rapid diagnostic test (RDT) (Seattle, USA) for the detection of B. pseudomallei in grown blood culture broth. The InBiOS RDT is a lateral flow immunoassay in a strip format detecting B. pseudomallei capsular polysaccharide in culture fluids, marketed for research only. Broth of blood culture bottles (BacT/Alert, bioMérieux, Marcy L'Etoile, France) sampled in adult patients at the Sihanouk Hospital Center of HOPE, Phnom Penh, Cambodia, during 2010-2017 and stored at - 80 °C was tested...
March 28, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29552530/molecular-characterization-and-antibiotic-susceptibility-pattern-of-acinetobacter-baumannii-isolated-in-intensive-care-unit-patients-in-al-hassa-kingdom-of-saudi-arabia
#14
Mohammed Sami Alhaddad, Afnan Khalifah AlBarjas, Lolowah Ebraheem Alhammar, Abdullatif Sami Al Rashed, Lorina Ineta Badger-Emeka
Background: Acinetobacter baumannii , is an emerging nosocomial multidrug resistance pathogen with the rapid spread of clones being reported in health-care settings and hospitals worldwide. Carbapenem resistance in this bacterium has been attributed to D OXA β-lactamases with OXA-51-like β-lactamase, being present in all A. baumannii isolate. The present study looks into the antibiotics susceptibility and molecular characterization of clinical A. baumannii isolates from Intensive Care Unit (ICU) samples in Al-Hofuf, South-eastern region of Saudi Arabia...
January 2018: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/29533176/characterisation-of-streptococcus-pneumoniae-isolates-from-invasive-disease-in-adults-following-the-introduction-of-pcv10-in-brazil
#15
Bernard Louizette Christophe, Mariana Mott, Gabriela da Cunha, Juliana Caierão, Pedro D Azevedo, Cícero Dias
PURPOSE: Invasive pneumococcal disease (IPD) in the elderly is an important public health issue due to the increased proportion of this population in many countries including Brazil. We aimed to characterise pneumococci isolates in adults >50 years with IPD, following the introduction of the 10-valent pneumococcal conjugate vaccine (PCV10) as part of the National Childhood Immunisation Program for children ≤2 years in March 2010. METHODOLOGY: Between 2013 and 2015, pneumococcal isolates were collected and serotypes were determined using multiplex PCR and/or Quellung reaction...
March 13, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29533172/field-trials-of-blood-culture-identification-filmarray-in-regional-australian-hospitals
#16
Nicole M Bzdyl, Nadezda Urosevic, Ben Payne, Ray Brockenshire, Michael McIntyre, Michael J Leung, Graham Weaire-Buchanan, Elizabeth Geelhoed, Timothy J J Inglis
PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories. METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure...
March 13, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29486357/antimicrobial-susceptibility-testing-of-finnish-bordetella-pertussis-isolates-collected-during-2006-2017
#17
Emilia Lönnqvist, Alex-Mikael Barkoff, Jussi Mertsola, Qiushui He
OBJECTIVES: Macrolides, such as azithromycin and erythromycin, are the first line drug for (prophylactic) treatment of pertussis. This study was aimed to screen for macrolide, quinolone, or trimethoprim-sulfamethoxazole resistant strains among Finnish Bordetella pertussis isolates. METHODS: Antimicrobial susceptibility testing was performed on 148 B. pertussis strains isolated during 2006-2017. Isolates were analysed with the allele-specific PCR for detection of macrolide resistance associated mutation A2047G in the 23S rRNA gene...
February 24, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29480795/comparison-of-two-commercial-quantitative-pcr-assays-for-ebv-dna-detection-and-their-correlation-with-the-first-who-international-standard-for-ebv
#18
COMPARATIVE STUDY
Ilka Engelmann, Enagnon Kazali Alidjinou, Mouna Lazrek, Jean-Marie Pouillaude, Judith Ogiez, François Rose, Alain Duhamel, Anny Dewilde, Didier Hober
PURPOSE: There are few data on the performance of automated Epstein-Barr virus (EBV) PCR assays. This study compared EBV quantification for the kPCR PLX EBV DNA (kPCR; Siemens, France) and the EBV R-gene (R-gene; Argene, Biomerieux, France) assays and their correlation with the World Health Organization (WHO) standard. METHODOLOGY: WHO International Standard for EBV (WHO standard) dilution panels in different matrices were submitted to nucleic acid extraction with Versant kPCR Molecular Systems SP followed by the kPCR assay, or to nucleic acid extraction with the MagNA Pure LC System or NucliSENS easyMag followed by the R-gene assay...
April 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29479341/clinically-relevant-esbl-producing-k-pneumoniae-st307-and-e-coli-st38-in-an-urban-west-african-rat-population
#19
Katharina Schaufler, Kathrin Nowak, Ariane Düx, Torsten Semmler, Laura Villa, Laye Kourouma, Karim Bangoura, Lothar H Wieler, Fabian H Leendertz, Sebastian Guenther
High-risk ESBL-producing Enterobacteriaceae (ESBL-E) have been described in wild birds and rodents worldwide. Rats are of special interest not only due to their indicator role for environmental pollution with multi-resistant bacteria but also as possible infection source. Data on the presence of high-risk ESBL-E in urban wildlife from Africa remain scarce, however. Twenty-nine animals from three different rat ( Rattus ) species were captured in the city of Conakry (Guinea, West Africa) in 2015. Rectal swabs were analyzed for ESBL-E using selective media...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29474660/performance-of-the-accelerate-pheno%C3%A2-system-for-identification-and-antimicrobial-susceptibility-testing-of-a-panel-of-multidrug-resistant-gram-negative-bacilli-directly-from-positive-blood-cultures
#20
Alix Pantel, Julien Monier, Jean-Philippe Lavigne
Objectives: To evaluate the performance of the Accelerate Pheno™ system for the identification and antimicrobial susceptibility testing (AST) of a panel of Gram-negative bacilli (GNB) with different resistance profiles (e.g. penicillinases, ESBLs, cephalosporinase overproduction, carbapenemases, impermeability) directly from positive blood cultures in <7 h. Methods: A panel of 105 clinical strains previously characterized for the presence of β-lactamase-encoding genes was tested...
February 20, 2018: Journal of Antimicrobial Chemotherapy
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