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https://www.readbyqxmd.com/read/28062628/specificity-of-b-type-natriuretic-peptide-assays-cross-reactivity-with-different-bnp-nt-probnp-and-probnp-peptides
#1
Amy K Saenger, Olaia Rodriguez-Fraga, Ranka Ler, Jordi Ordonez-Llanos, Allan S Jaffe, Jens Peter Goetze, Fred S Apple
BACKGROUND: B-type natriuretic peptides (BNPs) are used clinically to diagnose and monitor heart failure and are present in the circulation as multiple proBNP-derived fragments. We investigated the specificity of BNP immunoassays with glycosylated and nonglycosylated BNP, N-terminal proBNP (NT-proBNP), and proBNP peptides to probe the cross-reactivity of each assay. METHODS: Nine B-type natriuretic peptides were studied,including synthetic and recombinant BNP (Shionogi, Scios, Mayo), human and synthetic glycosylated and nonglycosylated NT-proBNP (HyTest, Roche Diagnostics), and human glycosylated and nonglycosylated proBNP (HyTest, Scios)...
January 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28035224/enzymatic-activity-of-fungi-isolated-from-crops
#2
Wioletta A Żukiewicz-Sobczak, Grażyna Cholewa, Paweł Sobczak, Wojciech Silny, Rafał Nadulski, Paulina Wojtyła-Buciora, Jerzy Zagórski
AIM: To detect and assess the activity of extracellular hydrolytic enzymes and to find differences in enzymograms between fungi isolated from wheat and rye samples and grown on Czapek-Dox Broth and Sabouraud Dextrose Broth enriched with cereal (wheat or rye). Isolated strains were also classified in the scale of biosafety levels (BSL). MATERIAL AND METHODS: The study used 23 strains of fungi cultured from samples of wheat and rye (grain, grain dust obtained during threshing and soil) collected in the Lublin region (eastern Poland)...
December 2016: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28031271/voriconazole-mics-are-predictive-for-the-outcome-of-experimental-disseminated-scedosporiosis
#3
Adela Martin-Vicente, Josep Guarro, Gloria M González, Cornelia Lass-Flörl, Michaela Lackner, Javier Capilla
BACKGROUND: Scedosporiosis is associated with a mortality rate of up to 90% in patients suffering from disseminated infections. Recommended first-line treatment is voriconazole, but epidemiological cut-off values and clinical breakpoints have not been determined. OBJECTIVES: To correlate voriconazole treatment response in mice suffering from disseminated scedosporiosis with MIC values determined using CLSI broth microdilution, Etest (bioMérieux) and disc diffusion...
December 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27995865/the-molecular-epidemiology-of-pseudomonas-aeruginosa-bloodstream-infection-isolates-in-a-non-outbreak-setting
#4
Kate L McCarthy, Tim J Kidd, David L Paterson
The molecular epidemiology of Pseudomonas aeruginosa bloodstream infection (BSI) isolates has received limited attention. This study aims to characterise the molecular relationship of P. aeruginosa bloodstream infection isolates in the non-outbreak setting at a single tertiary healthcare facility. P. aeruginosa BSI isolates from patients who were admitted to the Royal Brisbane and Women's Hospital over a 13 month period from November 2009 were identified retrospectively from the Pathology Queensland Clinical and Scientific Information System...
December 19, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27988132/survival-and-detection-of-coliforms-enterobacteriaceae-and-gram-negative-bacteria-in-greek-yogurt
#5
C J Hervert, N H Martin, K J Boor, M Wiedmann
Despite the widespread use of coliforms as indicator bacteria, increasing evidence suggests that the Enterobacteriaceae (EB) and total gram-negative groups more accurately reflect the hygienic status of high-temperature, short-time pasteurized milk and processing environments. If introduced into milk as postpasteurization contamination, these bacteria may grow to high levels and produce a wide range of sensory-related defects. However, limited information is available on the use and survival of bacterial hygiene indicators in dairy products outside of pasteurized fluid milk and cheese...
December 14, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27987422/diagnostic-performance-of-serological-assays-for-anti-hbs-testing-results-from-a-quality-assessment-program
#6
Stijn Raven, Jeannine Hautvast, Jim van Steenbergen, Reinier Akkermans, Cas Weykamp, Francis Smits, Christian Hoebe, Ann Vossen
BACKGROUND: Post-vaccination testing after hepatitis B vaccination is indispensable to evaluate long-term immunological protection. Using a threshold level of antibodies against hepatitis B surface antigen (anti-HBs) to define serological protection, implies reproducible and valid measurements of different diagnostic assays. OBJECTIVES: In this study we assess the performance of currently used anti-HBs assays. STUDY DESIGN: In 2013, 45 laboratories participated in an external quality assessment program using pooled anti-HBs serum samples around the cutoff values 10IU/l and 100IU/l...
December 7, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27987046/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-rapid-identification-of-micro-organisms-in-the-routine-clinical-microbiology-laboratory
#7
C Wattal, J K Oberoi, N Goel, R Raveendran, S Khanna
The study evaluates the utility of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) Vitek MS for identification of microorganisms in the routine clinical microbiology laboratory. From May 2013 to April 2014, microbial isolates recovered from various clinical samples were identified by Vitek MS. In case of failure to identify by Vitek MS, the isolate was identified using the Vitek 2 system (bioMerieux, France) and serotyping wherever applicable or otherwise by nucleic acid-mediated methods...
December 16, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27955639/control-of-carbapenem-resistant-acinetobacter-baumannii-outbreak-in-an-intensive-care-unit-of-a-teaching-hospital-in-southern-italy
#8
Aida Bianco, Angela Quirino, Mariavalentina Giordano, Vito Marano, Claudia Rizzo, Maria Carla Liberto, Alfredo Focà, Maria Pavia
BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen that has become a major cause of concern, since it is a frequent cause of healthcare-associated infections (HAIs). The aim of the study was to describe the occurrence, the management and the control of an outbreak that occurred in an intensive care unit (ICU) of a teaching hospital in Southern Italy caused by multiple strains of extensively drug-resistant A. baumannii (XDRAB). METHODS: Case-patient was defined as a patient with an healthcare-associated infection caused by an XDRAB isolate identified in a clinically significant culture...
December 12, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27934842/infections-related-to-granulicatella-adiacens-report-of-two-cases-and-review-of-literature
#9
S Macin, A Ç İnkaya, Ö Tuncer, S Ünal, Y Akyön
Infections due to nutritionally variant streptococci are diagnosed rarely due to difficulties encountered during identification and isolation. Mortality rate in these infections is high therefore appropriate supplemented media and reliable detection systems should be implemented to isolate these fastidious organisms. Here, we describe two cases of Granulicatella adiacens infections. All microbiologic identifications were made with MALDI-TOF Vitek MS (BioMerieux, France), and the results confirmed by 16S ribotyping...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27927915/comparative-evaluation-of-four-phenotypic-tests-for-the-detection-of-carbapenemase-producing-gram-negative-bacteria
#10
Audrey Noël, Te-Din Huang, Catherine Berhin, Martin Hoebeke, Warda Bouchahrouf, Sami Yunus, Pierre Bogaerts, Youri Glupczynski
Four screening assays aimed for rapid detection of carbapenemase production from Gram-negative bacterial isolates i.e. the Neo-Rapid CARB kit (Rosco Diagnostica A/S), the RAPIDEC CARBA NP test (BioMérieux SA), the β CARBA test (Bio-Rad Laboratories N.V) and a home-made electrochemical assay (BYG Carba test) were evaluated against a panel comprising 328 clinical isolates (Enterobacteriaceae [n= 198], nonfermentative Gram-negative bacilli [n = 130]) with previously characterized resistance mechanisms to carbapenems...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27904747/comparing-the-disk-diffusion-and-agar-dilution-tests-for-neisseria-gonorrhoeae-antimicrobial-susceptibility-testing
#11
Hsi Liu, Thomas H Taylor, Kevin Pettus, Steve Johnson, John R Papp, David Trees
BACKGROUND: We assessed the validity of testing for antimicrobial susceptibility of clinical and mutant Neisseria gonorrhoeae (GC) isolates by disk diffusion in comparison to agar dilution, and Etest® (bioMerieux, France), respectively, for three third generation extended spectrum cephalosporins (ESC): ceftriaxone (CRO), cefixime (CFX), and cefpodoxime (CPD). METHODS: One hundred and five clinical isolates and ten laboratory-mutants were tested following Clinical Laboratory Standard Institute (CLSI) and manufacturer's standards for each of the three methods...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27902602/sensitivity-and-specificity-of-dried-blood-spots-for-hiv-1-viral-load-quantification-a-laboratory-assessment-of-3-commercial-assays
#12
Pieter Pannus, Maarten Claus, Maria Mercedes Perez Gonzalez, Nathan Ford, Katrien Fransen
The use of dried blood spots (DBS) instead of plasma as a specimen type for HIV-1 viral load (VL) testing facilitates the decentralization of specimen collection and can increase access to VL testing in resource-limited settings. The performance of DBS for VL testing is lower, however, when compared to the gold standard sample type plasma. In this diagnostic accuracy study, we evaluated 3 VL assays with DBS.Participants were recruited between August 2012 and April 2015. Both plasma and DBS specimens were prepared and tested for HIV-1 VL with the Roche CAP/CTM HIV-1 test v2...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27853518/metagenomic-analysis-of-medicinal-cannabis-samples-pathogenic-bacteria-toxigenic-fungi-and-beneficial-microbes-grow-in-culture-based-yeast-and-mold-tests
#13
Kevin McKernan, Jessica Spangler, Yvonne Helbert, Ryan C Lynch, Adrian Devitt-Lee, Lei Zhang, Wendell Orphe, Jason Warner, Theodore Foss, Christopher J Hudalla, Matthew Silva, Douglas R Smith
Background: The presence of bacteria and fungi in medicinal or recreational Cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. This study evaluated two widely used culture-based platforms for total yeast and mold (TYM) testing marketed by 3M Corporation and Biomérieux, in comparison with a quantitative PCR (qPCR) approach marketed by Medicinal Genomics Corporation. Methods: A set of 15 medicinal Cannabis samples were analyzed using 3M and Biomérieux culture-based platforms and by qPCR to quantify microbial DNA...
2016: F1000Research
https://www.readbyqxmd.com/read/27852676/detection-of-clostridium-difficile-in-feces-of-asymptomatic-patients-admitted-to-the-hospital
#14
Elisabeth M Terveer, Monique J T Crobach, Ingrid M J G Sanders, Margreet C Vos, Cees M Verduin, Ed J Kuijper
Recent evidence shows that patients asymptomatically colonized with Clostridium difficile may contribute to the transmission of C. difficile in healthcare facilities. Additionally, these patients may have a higher risk of developing C. difficile infection. The aim of this study was to compare a commercially available PCR directed to both toxin A as B (artus C. difficile QS-RGQ Kit CE, QIAGEN), an Enzyme Linked Fluorescent Assay to Glutamate Dehydrogenase (GDH ELFA, VIDAS, bioMérieux), and an in-house developed PCR to TcdB, with (toxigenic) culture of C...
November 16, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27846254/extended-spectrum-beta-%C3%AE-lactamases-and-antibiogram-in-enterobacteriaceae-from-clinical-and-drinking-water-sources-from-bahir-dar-city-ethiopia
#15
Bayeh Abera, Mulugeta Kibret, Wondemagegn Mulu
BACKGROUND: The spread of Extended-Spectrum beta (β)-Lactamases (ESBL)-producing Enterobacteriaceae has become a serious global problem. ESBL-producing Enterobacteriaceae vary based on differences in antibiotic use, nature of patients and hospital settings. This study was aimed at determining ESBL and antibiogram in Enterobacteriaceae isolates from clinical and drinking water sources in Bahir Dar City, Northwest Ethiopia. METHODS: Enterobacteriaceae species were isolated from clinical materials and tap water using standard culturing procedures from September 2013 to March 2015...
2016: PloS One
https://www.readbyqxmd.com/read/27830020/evaluation-of-a-research-use-only-luminex-based-assay-for-measurement-of-procalcitonin-in-serum-samples
#16
Charles Garrigan, Jennifer Han, Pam Tolomeo, Katherine J Johnson, Stephen R Master, Ebbing Lautenbach, Irving Nachamkin
Research use only (RUO) assays do not undergo a validation process similar to test kits used for clinical purposes. Several studies have suggested that RUO assays need to be validated prior to use in any research studies. We evaluated a research use only Luminex platform based assay for measuring serum procalcitonin levels (Bio-Plex Pro(TM) Human Acute Phase Multiplex Assay, Bio-Rad Laboratories, Hercules, CA) for comparability with an FDA cleared assay for procalcitonin (VIDAS B.R.A.H.M.S. PCT Assay, bioMérieux, Durham, NC)...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27821277/comparison-of-different-approaches-to-quantitative-adenovirus-detection-in-stool-specimens-of-hematopoietic-stem-cell-transplant-recipients
#17
K Kosulin, S Dworzak, A Lawitschka, S Matthes-Leodolter, T Lion
BACKGROUND: Adenoviruses almost invariably proliferate in the gastrointestinal tract prior to dissemination, and critical threshold concentrations in stool correlate with the risk of viremia. Monitoring of adenovirus loads in stool may therefore be important for timely initiation of treatment in order to prevent invasive infection. OBJECTIVES: Comparison of a manual DNA extraction kit in combination with a validated in-house PCR assay with automated extraction on the NucliSENS-EasyMAG device coupled with the Adenovirus R-gene kit (bioMérieux) for quantitative adenovirus analysis in stool samples...
December 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27818808/persistent-bacteremia-from-pseudomonas-aeruginosa-with-in-vitro-resistance-to-the-novel-antibiotics-ceftolozane-tazobactam-and-ceftazidime-avibactam
#18
Louie Mar Gangcuangco, Patricia Clark, Cynthia Stewart, Goran Miljkovic, Zane K Saul
Ceftazidime-avibactam and ceftolozane-tazobactam are new antimicrobials with activity against multidrug-resistant Pseudomonas aeruginosa. We present the first case of persistent P. aeruginosa bacteremia with in vitro resistance to these novel antimicrobials. A 68-year-old man with newly diagnosed follicular lymphoma was admitted to the medical intensive care unit for sepsis and right lower extremity cellulitis. The patient was placed empirically on vancomycin and piperacillin-tazobactam. Blood cultures from Day 1 of hospitalization grew P...
2016: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/27816058/assessment-of-human-papillomavirus-e6-e7-oncogene-expression-as-cervical-disease-biomarker
#19
Nerea Fontecha, Miren Basaras, Silvia Hernáez, Daniel Andía, Ramón Cisterna
BACKGROUND: The aims of this study were to detect HPV E6/E7 mRNA expression in women with high-risk genotypes (HPV-16, -18, -31, -33 and -45) analysing its relationship with tissue pathology and 2) 2-year follow-up of E6/E7 mRNA tested group. METHODS: Our samples were genotyped and classified by pathologists according to Bethesda system. After RNA extraction, E6/E7 oncogene mRNA detection was performed by NucliSens® EasyQ® HPV v1 Test (bioMérieux). RESULTS: The results of the present study showed that E6/E7 mRNA positivity rate was 68...
November 5, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27805525/microbiologic-investigation-of-patients-before-living-donor-liver-transplant
#20
Nelya Bissenova, Aigerim Yergaliyeva
OBJECTIVES: Chronic infections can cause serious complications and create morbidity and mortality for affected patients during immunosuppressive therapy in the posttransplant period. Here, we studied the microbiologic screening results of patients before living-donor liver transplant. MATERIALS AND METHODS: The microbiologic screening results of 25 patients during 2015 at National Scientific Research Center (Astana, Kazakhstan) before livingdonor liver transplant were prospectively analyzed...
2016: Experimental and Clinical Transplantation
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