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https://www.readbyqxmd.com/read/27904747/comparing-the-disk-diffusion-and-agar-dilution-tests-for-neisseria-gonorrhoeae-antimicrobial-susceptibility-testing
#1
Hsi Liu, Thomas H Taylor, Kevin Pettus, Steve Johnson, John R Papp, David Trees
BACKGROUND: We assessed the validity of testing for antimicrobial susceptibility of clinical and mutant Neisseria gonorrhoeae (GC) isolates by disk diffusion in comparison to agar dilution, and Etest® (bioMerieux, France), respectively, for three third generation extended spectrum cephalosporins (ESC): ceftriaxone (CRO), cefixime (CFX), and cefpodoxime (CPD). METHODS: One hundred and five clinical isolates and ten laboratory-mutants were tested following Clinical Laboratory Standard Institute (CLSI) and manufacturer's standards for each of the three methods...
2016: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/27902602/sensitivity-and-specificity-of-dried-blood-spots-for-hiv-1-viral-load-quantification-a-laboratory-assessment-of-3-commercial-assays
#2
Pieter Pannus, Maarten Claus, Maria Mercedes Perez Gonzalez, Nathan Ford, Katrien Fransen
The use of dried blood spots (DBS) instead of plasma as a specimen type for HIV-1 viral load (VL) testing facilitates the decentralization of specimen collection and can increase access to VL testing in resource-limited settings. The performance of DBS for VL testing is lower, however, when compared to the gold standard sample type plasma. In this diagnostic accuracy study, we evaluated 3 VL assays with DBS.Participants were recruited between August 2012 and April 2015. Both plasma and DBS specimens were prepared and tested for HIV-1 VL with the Roche CAP/CTM HIV-1 test v2...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27853518/metagenomic-analysis-of-medicinal-cannabis-samples-pathogenic-bacteria-toxigenic-fungi-and-beneficial-microbes-grow-in-culture-based-yeast-and-mold-tests
#3
Kevin McKernan, Jessica Spangler, Yvonne Helbert, Ryan C Lynch, Adrian Devitt-Lee, Lei Zhang, Wendell Orphe, Jason Warner, Theodore Foss, Christopher J Hudalla, Matthew Silva, Douglas R Smith
Background: The presence of bacteria and fungi in medicinal or recreational Cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. This study evaluated two widely used culture-based platforms for total yeast and mold (TYM) testing marketed by 3M Corporation and Biomérieux, in comparison with a quantitative PCR (qPCR) approach marketed by Medicinal Genomics Corporation. Methods: A set of 15 medicinal Cannabis samples were analyzed using 3M and Biomérieux culture-based platforms and by qPCR to quantify microbial DNA...
2016: F1000Research
https://www.readbyqxmd.com/read/27852676/detection-of-clostridium-difficile-in-feces-of-asymptomatic-patients-admitted-to-the-hospital
#4
Elisabeth M Terveer, Monique J T Crobach, Ingrid M J G Sanders, Margreet C Vos, Cees M Verduin, Ed J Kuijper
Recent evidence shows that patients asymptomatically colonized with Clostridium difficile may contribute to the transmission of C. difficile in healthcare facilities. Additionally, these patients may have a higher risk of developing C. difficile infection. The aim of this study was to compare a commercially available PCR directed to both toxin A as B (artus C. difficile QS-RGQ Kit CE, QIAGEN), an Enzyme Linked Fluorescent Assay to Glutamate Dehydrogenase (GDH ELFA, VIDAS, bioMérieux), and an in-house developed PCR to TcdB, with (toxigenic) culture of C...
November 16, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27846254/extended-spectrum-beta-%C3%AE-lactamases-and-antibiogram-in-enterobacteriaceae-from-clinical-and-drinking-water-sources-from-bahir-dar-city-ethiopia
#5
Bayeh Abera, Mulugeta Kibret, Wondemagegn Mulu
BACKGROUND: The spread of Extended-Spectrum beta (β)-Lactamases (ESBL)-producing Enterobacteriaceae has become a serious global problem. ESBL-producing Enterobacteriaceae vary based on differences in antibiotic use, nature of patients and hospital settings. This study was aimed at determining ESBL and antibiogram in Enterobacteriaceae isolates from clinical and drinking water sources in Bahir Dar City, Northwest Ethiopia. METHODS: Enterobacteriaceae species were isolated from clinical materials and tap water using standard culturing procedures from September 2013 to March 2015...
2016: PloS One
https://www.readbyqxmd.com/read/27830020/evaluation-of-a-research-use-only-luminex-based-assay-for-measurement-of-procalcitonin-in-serum-samples
#6
Charles Garrigan, Jennifer Han, Pam Tolomeo, Katherine J Johnson, Stephen R Master, Ebbing Lautenbach, Irving Nachamkin
Research use only (RUO) assays do not undergo a validation process similar to test kits used for clinical purposes. Several studies have suggested that RUO assays need to be validated prior to use in any research studies. We evaluated a research use only Luminex platform based assay for measuring serum procalcitonin levels (Bio-Plex Pro(TM) Human Acute Phase Multiplex Assay, Bio-Rad Laboratories, Hercules, CA) for comparability with an FDA cleared assay for procalcitonin (VIDAS B.R.A.H.M.S. PCT Assay, bioMérieux, Durham, NC)...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27821277/comparison-of-different-approaches-to-quantitative-adenovirus-detection-in-stool-specimens-of-hematopoietic-stem-cell-transplant-recipients
#7
K Kosulin, S Dworzak, A Lawitschka, S Matthes-Leodolter, T Lion
BACKGROUND: Adenoviruses almost invariably proliferate in the gastrointestinal tract prior to dissemination, and critical threshold concentrations in stool correlate with the risk of viremia. Monitoring of adenovirus loads in stool may therefore be important for timely initiation of treatment in order to prevent invasive infection. OBJECTIVES: Comparison of a manual DNA extraction kit in combination with a validated in-house PCR assay with automated extraction on the NucliSENS-EasyMAG device coupled with the Adenovirus R-gene kit (bioMérieux) for quantitative adenovirus analysis in stool samples...
November 1, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27818808/persistent-bacteremia-from-pseudomonas-aeruginosa-with-in-vitro-resistance-to-the-novel-antibiotics-ceftolozane-tazobactam-and-ceftazidime-avibactam
#8
Louie Mar Gangcuangco, Patricia Clark, Cynthia Stewart, Goran Miljkovic, Zane K Saul
Ceftazidime-avibactam and ceftolozane-tazobactam are new antimicrobials with activity against multidrug-resistant Pseudomonas aeruginosa. We present the first case of persistent P. aeruginosa bacteremia with in vitro resistance to these novel antimicrobials. A 68-year-old man with newly diagnosed follicular lymphoma was admitted to the medical intensive care unit for sepsis and right lower extremity cellulitis. The patient was placed empirically on vancomycin and piperacillin-tazobactam. Blood cultures from Day 1 of hospitalization grew P...
2016: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/27816058/assessment-of-human-papillomavirus-e6-e7-oncogene-expression-as-cervical-disease-biomarker
#9
Nerea Fontecha, Miren Basaras, Silvia Hernáez, Daniel Andía, Ramón Cisterna
BACKGROUND: The aims of this study were to detect HPV E6/E7 mRNA expression in women with high-risk genotypes (HPV-16, -18, -31, -33 and -45) analysing its relationship with tissue pathology and 2) 2-year follow-up of E6/E7 mRNA tested group. METHODS: Our samples were genotyped and classified by pathologists according to Bethesda system. After RNA extraction, E6/E7 oncogene mRNA detection was performed by NucliSens® EasyQ® HPV v1 Test (bioMérieux). RESULTS: The results of the present study showed that E6/E7 mRNA positivity rate was 68...
November 5, 2016: BMC Cancer
https://www.readbyqxmd.com/read/27805525/microbiologic-investigation-of-patients-before-living-donor-liver-transplant
#10
Nelya Bissenova, Aigerim Yergaliyeva
OBJECTIVES: Chronic infections can cause serious complications and create morbidity and mortality for affected patients during immunosuppressive therapy in the posttransplant period. Here, we studied the microbiologic screening results of patients before living-donor liver transplant. MATERIALS AND METHODS: The microbiologic screening results of 25 patients during 2015 at National Scientific Research Center (Astana, Kazakhstan) before livingdonor liver transplant were prospectively analyzed...
November 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27805513/microbiologic-monitoring-of-kidney-transplant-recipients-before-transplant
#11
Nelya Bissenova, Aigerim Yergaliyeva
OBJECTIVES: Drug immunosuppression, traditionally used to minimize autoimmune developments after liver, kidney, heart, and lung transplant, and which requires high doses of corticosteroids, creates favorable conditions for the development of various infectious processes and the existence of pathogens, which can be activated after transplant. This study aimed to investigate the bacterial pathogens in patients before living-donor kidney transplant. MATERIALS AND METHODS: We prospectively investigated 117 clinical samples of 33 patients who were seen during 2015 at the National Scientific Research Center (Astana, Kazakhstan) before living-donor kidney transplant...
November 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27798220/etest%C3%A2-versus-broth-microdilution-for-ceftaroline-mic-determination-with-staphylococcus-aureus-results-from-premium-a-european-multicentre-study
#12
Rafael Cantón, David M Livermore, María Isabel Morosini, Jazmín Díaz-Regañón, Gian Maria Rossolini
OBJECTIVES: To compare the concordance of ceftaroline MIC values by reference broth microdilution (BMD) and Etest (bioMérieux, France) for MSSA and MRSA isolates obtained from PREMIUM (D372SL00001), a European multicentre study. METHODS: Ceftaroline MICs were determined by reference BMD and by Etest for 1242 MSSA and MRSA isolates collected between February and May 2012 from adult patients with community-acquired pneumonia or complicated skin and soft tissue infections; tests were performed across six European laboratories...
October 20, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27796646/streptococcus-bovis-new-taxonomy-does-subspecies-distinction-matter
#13
E Ben-Chetrit, Y Wiener-Well, L Kashat, A M Yinnon, M V Assous
Bacteremia with Streptococcus bovis/equinus complex strains is associated with hepatobiliary disease, colorectal lesions (CL), and infective endocarditis (IE). This study addressed the clinical significance of subspecies distinction of previously designated S. bovis blood culture isolates according to the updated nomenclature. During 2002-2013, all blood culture isolates previously designated as S. bovis were recultured and identified using 16S rRNA gene sequencing and MALDI-TOF (Bruker BioTyper and Vitek MS, bioMérieux)...
October 28, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27790432/invitro-activities-of-polymyxins-and-rifampicin-against-carbapenem-resistant-acinetobacter-baumannii-at-a-tertiary-care-hospital-from-south-india
#14
Dheepa Muthusamy, S Sudhishnaa, Appalaraju Boppe
INTRODUCTION: Acinetobacter baumannii (A.baumannii) is rapidly emerging as a potent organism causing a multitude of nosocomial infections. The organism also carries various resistance mechanisms to antibiotics, making treatment more difficult. Very few choices are left, as A.baumannii strains have begun to develop resistance against cephalosporins, aminoglycosides and even carbapenems. AIM: To examine the sensitivity pattern of three older antibiotics namely colistin, polymyxin B and rifampicin against carbapenem resistant A...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27770760/the-hydrolytic-enzymes-produced-by-fungi-strains-isolated-from-the-sand-and-soil-of-recreational-areas
#15
Piotr Kurnatowski, Anna Wójcik, Joanna Błaszkowska, Katarzyna Góralska
The pathogenicity of fungi depends on, inter alia, the secretion of hydrolytic enzymes. The aim of this study was to determine the enzymatic activity of yeasts and yeast-like fungi isolated from children’s recreation areas, and compare the results with literature data of strains obtained from patients with mycoses. The enzymatic activity of 96 strains was assessed using an API ZYM kit (bioMerieux, France) and their biotypes were established. The fungal species were found to produce from 16 to 19 hydrolases: the most active were: leucine arylamidase (e5), acid phosphatase (e10), alkaline phosphatase (e1), naphthol-AS-BI-phosphohydrolase (e11), esterase – C4 (e2), β-galac - tosidase (e13) and β-glucosidase (e16)...
October 1, 2016: Annals of Parasitology
https://www.readbyqxmd.com/read/27770528/bacteriological-examination-of-the-abdominal-effusion-in-bacterial-peritonitis
#16
T Didbaridze, L Saginashvili, L Akhmeteli, B Iremashvili, N Gogokhia
This study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. Our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (API20E, API Staph, API Strep, API Ana, BioMerieux). Rapid tests for detection of oxidase and catalase activity were also used. Susceptibility of microorganisms to antibiotics was defined by the disc-diffusion method using standard discs (EUCAST guidelines 2015) according to Clinical Laboratory Standard Institute (CLSI) protocols (ATB strips: ATB G, ATB Staph, ATBANA, ATBPse, ATBStrep...
September 2016: Georgian Medical News
https://www.readbyqxmd.com/read/27766170/laboratory-experience-with-the-liaison-analyzer-in-the-diagnosis-of-clostridium-difficile-associated-diarrhea
#17
Orsolya Benedek, Andreas Podbielski, Philipp Warnke
BACKGROUND: Chemiluminescent or enzyme-linked fluorescent immunoassays are commonly used to diagnose Clostridium difficile-associated diarrhea. METHODS: The LIAISON analyzer (DiaSorin, Italy) was compared to miniVIDAS (bioMérieux, France) and, furthermore, to culture of toxigenic strains. In total, 249 native stool samples were analyzed. Sensitivities, specificities, and positive and negative predictive values were investigated. Furthermore, performance under routine conditions was assessed...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27762213/comparison-of-toxoplasma-gondii-igg-avidity-architect-and-vidas-assays-with-the-estimated-date-of-infection-in-pregnant-women
#18
Aurélie Smets, Thomas Fauchier, Grégory Michel, Pierre Marty, Christelle Pomares
A maternal Toxoplasma gondii infection during pregnancy is a risk for congenital infection through maternal-fetal transplacental transmission. Estimation of the date of infection is of the utmost importance for management and treatment recommendations. In this setting, IgG avidity has been shown to be useful as high avidity rules out an infection dating less than 4 months. The estimated date of infection can also be obtained by the ratio of T. gondii IgG titers measured by the Vidas (bioMérieux) assay versus T...
2016: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/27702604/evaluation-of-maldi-tof-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-routine-identification-of-anaerobic-bacteria
#19
Belén Rodríguez-Sánchez, Luis Alcalá, Mercedes Marín, Adrián Ruiz, Elena Alonso, Emilio Bouza
Information regarding the use of MALDI-TOF MS as an alternative to conventional laboratory methods for the rapid and reliable identification of bacterial isolates is still limited. In this study, MALDI-TOF MS was evaluated on 295 anaerobic isolates previously identified by 16S rRNA gene sequencing and with biochemical tests (Rapid ID 32A system, BioMérieux). In total, 85.8% of the isolates were identified by MALDI-TOF MS at the species level vs 49.8% using the Rapid ID 32A system (p < 0.0001). None of the isolates was discordantly identified at the genus level using MALDI-TOF MS and only 9 of them could not be identified using the method...
October 1, 2016: Anaerobe
https://www.readbyqxmd.com/read/27668898/antimicrobial-susceptibility-against-penicillin-ampicillin-and-vancomycin-of-viridans-group-streptococcus-in-oral-microbiota-of-patients-at-risk-of-infective-endocarditis
#20
Serap Süzük, Banu Kaşkatepe, Mustafa Çetin
The viridans group Streptococci (VGS) are most abundant in the mouth; in some instances they might emerge as pathogens particularly in infective endocarditis (IE). In this study, we aimed to define and determine the susceptibility against antibiotics of VGS that are members of the oral microbiota of patients exhibiting a risk of developing IE. Forty-nine patients at risk of infective endocarditis were included in the study. Identification of the bacteria was performed using API STREP (bioMérieux, France). Gradient test strips (E-Test, France) were used to determine MIC of the bacteria against penicillin, ampicillin, and vancomycin...
September 1, 2016: Le Infezioni in Medicina
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