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Bernard Louizette Christophe, Mariana Mott, Gabriela da Cunha, Juliana Caierão, Pedro D Azevedo, Cícero Dias
PURPOSE: Invasive pneumococcal disease (IPD) in the elderly is an important public health issue due to the increased proportion of this population in many countries including Brazil. We aimed to characterise pneumococci isolates in adults >50 years with IPD, following the introduction of the 10-valent pneumococcal conjugate vaccine (PCV10) as part of the National Childhood Immunisation Program for children ≤2 years in March 2010. METHODOLOGY: Between 2013 and 2015, pneumococcal isolates were collected and serotypes were determined using multiplex PCR and/or Quellung reaction...
March 13, 2018: Journal of Medical Microbiology
Nicole M Bzdyl, Nadezda Urosevic, Ben Payne, Ray Brockenshire, Michael McIntyre, Michael J Leung, Graham Weaire-Buchanan, Elizabeth Geelhoed, Timothy J J Inglis
PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories. METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure...
March 13, 2018: Journal of Medical Microbiology
Emilia Lönnqvist, Alex-Mikael Barkoff, Jussi Mertsola, Qiushui He
OBJECTIVES: Macrolides, such as azithromycin and erythromycin, are the first line drug for (prophylactic) treatment of pertussis. This study was aimed to screen for macrolide, quinolone, or trimethoprim-sulfamethoxazole resistant strains among Finnish Bordetella pertussis isolates. METHODS: Antimicrobial susceptibility testing was performed on 148 B. pertussis strains isolated during 2006-2017. Isolates were analysed with the allele-specific PCR for detection of macrolide resistance associated mutation A2047G in the 23S rRNA gene...
February 24, 2018: Journal of Global Antimicrobial Resistance
Ilka Engelmann, Enagnon Kazali Alidjinou, Mouna Lazrek, Jean-Marie Pouillaude, Judith Ogiez, François Rose, Alain Duhamel, Anny Dewilde, Didier Hober
PURPOSE: There are few data on the performance of automated Epstein-Barr virus (EBV) PCR assays. This study compared EBV quantification for the kPCR PLX EBV DNA (kPCR; Siemens, France) and the EBV R-gene (R-gene; Argene, Biomerieux, France) assays and their correlation with the World Health Organization (WHO) standard. METHODOLOGY: WHO International Standard for EBV (WHO standard) dilution panels in different matrices were submitted to nucleic acid extraction with Versant kPCR Molecular Systems SP followed by the kPCR assay, or to nucleic acid extraction with the MagNA Pure LC System or NucliSENS easyMag followed by the R-gene assay...
February 26, 2018: Journal of Medical Microbiology
Katharina Schaufler, Kathrin Nowak, Ariane Düx, Torsten Semmler, Laura Villa, Laye Kourouma, Karim Bangoura, Lothar H Wieler, Fabian H Leendertz, Sebastian Guenther
High-risk ESBL-producing Enterobacteriaceae (ESBL-E) have been described in wild birds and rodents worldwide. Rats are of special interest not only due to their indicator role for environmental pollution with multi-resistant bacteria but also as possible infection source. Data on the presence of high-risk ESBL-E in urban wildlife from Africa remain scarce, however. Twenty-nine animals from three different rat ( Rattus ) species were captured in the city of Conakry (Guinea, West Africa) in 2015. Rectal swabs were analyzed for ESBL-E using selective media...
2018: Frontiers in Microbiology
Alix Pantel, Julien Monier, Jean-Philippe Lavigne
Objectives: To evaluate the performance of the Accelerate Pheno™ system for the identification and antimicrobial susceptibility testing (AST) of a panel of Gram-negative bacilli (GNB) with different resistance profiles (e.g. penicillinases, ESBLs, cephalosporinase overproduction, carbapenemases, impermeability) directly from positive blood cultures in <7 h. Methods: A panel of 105 clinical strains previously characterized for the presence of β-lactamase-encoding genes was tested...
February 20, 2018: Journal of Antimicrobial Chemotherapy
Ruxin Li, Di Xiao, Jing Yang, Suju Sun, Samuel Kaplan, Zhirong Li, Yanan Niu, Cuixin Qiang, Yu Zhai, Xiaoming Wang, Xingzhen Zhao, Baoxin Zhao, Martin Welker, David H Pincus, Dazhi Jin, Mini Kamboj, Guanghui Zheng, Guojun Zhang, Jianzhong Zhang, Yi-Wei Tang, Jianhong Zhao
Clostridium difficile multilocus sequence type 37 (ST37), which mainly corresponds to ribotype 017, has been a dominant genotype circulating in China. In this study, we report the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze and characterize 204 C. difficile clinical isolates including 49 ST37 and 155 non-ST37 isolates collected in China and other countries. Distribution of two major protein peaks ( m/z 3242 and 3286) was significantly different between ST37 and non-ST37 prototype strains and clinical isolates...
February 21, 2018: Journal of Clinical Microbiology
Reema Nath, Reeta Bora, Biswajyoti Borkakoty, Lahari Saikia, Pratap Parida
In this retrospective study from 2012 to 2015, 333 clinical isolates of yeasts were identified using Vitek 2 Compact System YST ID card (Biomerieux, France) and internal transcribed spacer (ITS) sequencing. Eighteen species were identified by ITS sequencing. Candida albicans was the most common species (46.5%), followed by Candida tropicalis (27%). The total species supported by Vitek System was 11 (61.11%). The sensitivity of the system in identifying these 11 species was 66.66%-100%; specificity 98.37%-100%; positive predictive value 70%-100%, negative predictive value 96...
October 2017: Indian Journal of Medical Microbiology
Jihye Ha, Sung Kuk Hong, Geum Hee Han, Myungsook Kim, Dongeun Yong, Kyungwon Lee
BACKGROUND: Early and appropriate antibiotic treatment improves the clinical outcome of patients with septicemia; therefore, reducing the turn-around time for identification (ID) and antimicrobial susceptibility test (AST) results is essential. We established a method for rapid ID and AST using short-term incubation of positive blood culture broth samples on solid media, and evaluated its performance relative to that of the conventional method using two rapid ID systems and a rapid AST method...
May 2018: Annals of Laboratory Medicine
Lucia Sanchez Ramos, Arne C Rodloff
The genus Clostridium is of high clinical relevance, as some species may cause rapid and even lethal infections. Thus, a timely identification of these anaerobic bacteria is desirable. Conventional identification methods rely on biochemical properties of these organisms, however, establishing these is time-consuming and not always reliable. Alternatively, 16S rRNA gene sequence based diagnostic methods may be used, but they are expensive and not ubiquitously available. This study was designed to assess the possibility to identify Clostridium species employing the matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS)...
January 29, 2018: Anaerobe
Hélène Yera, Louise Ménégaut, Marie-Pierre Brenier-Pinchart, Feriel Touafek, Patrick Bastien, Frédéric Dalle
OBJECTIVES: Molecular detection of Toxoplasma gondii plays a crucial role in the prenatal and neonatal diagnosis of congenital toxoplasmosis (CT). Sensitivity of this diagnosis is partly related to the efficiency of parasite DNA extraction and amplification. DNA extraction methods with automated platforms have been developed. Therefore, it is essential to evaluate them in combination with adequate PCR amplification assays. METHODS: In this multisite study, we investigated the suitability of two recent automated procedures for the isolation of Toxoplasma DNA from amniotic fluid (AF) (Magtration system 12GC, PSS® and Freedom EVO VacS, Tecan®), as compared to three other automated procedures (MagNAPure Compact, Roche®, BioRobot EZ1, Qiagen® and modified NucliSens easyMAG, bioMérieux®) and to the manual DNA extraction QIAamp DNA Mini kit (Qiagen®)...
January 23, 2018: Clinical Microbiology and Infection
Iriagbonse I Osaigbovo, Patrick V Lofor, Rita O Oladele
Oropharyngeal candidiasis, a common fungal infection in people living with HIV/AIDS (PLWHA), arises from Candida species colonizing the oral cavity. Fluconazole is the preferred treatment and is often used empirically. Few studies have investigated the prevalence of fluconazole resistance in Nigeria. This study aimed at determining the burden of fluconazole resistance among Candida species in the oral cavities of PLWHA. We sampled the oral cavities of 350 HIV-infected adults and an equal number of HIV-negative controls...
December 8, 2017: Journal of Fungi (Basel, Switzerland)
Adelino da Cunha-Neto, Larrayane Albuês Carvalho, Ricardo César Tavares Carvalho, Dália Dos Prazeres Rodrigues, Sergio Borges Mano, Eduardo Eustáquio de Souza Figueiredo, Carlos Adam Conte-Junior
Salmonella is one of the major causative agents of foodborne infections. Salmonellosis becomes more dangerous when strains resistant to several antibiotics are found in food, especially in chicken, one of the primary transmission vehicles of this pathogen for humans. The present study aimed to estimate the occurrence of Salmonella in chicken carcasses from the state of Mato Grosso, Brazil, as well as determine the antibiotic resistance profile and genotypic characteristic of multi-drug resistant (MDR) isolates...
January 20, 2018: Poultry Science
Ellionor Rapp, Ørjan Samuelsen, Martin Sundqvist
This study evaluated the performance of the MBT STAR-Carba kit (Bruker Daltonics), to detect carbapenemase producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp. in comparison with the RAPIDEC® CARBA NP test (BioMerieux). MBT STAR-Carba allowed the detection of carbapenemases in Enterobacteriaceae and P. aeruginosa.
January 19, 2018: Journal of Microbiological Methods
Sophie Tiphaine Loeffert, Philippe Vanhems, Estelle Tissot, Cédric Dananché, Pierre Cassier, Thomas Bénet, Michel Perraud, Michel Thibaudon, Marie-Paule Gustin
Demolition can generate fungal spore suspensions in association with various adverse health effects, such as high risk of invasive aspergillosis in immunocompromised patients. One block of Edouard Herriot Hospital was entirely demolished. The aim of the present study was to evaluate Hirst-type spore traps utility in monitoring outdoor Aspergillaceae (Aspergillus spp. + Penicillium spp.) spores in part of Edouard Herriot Hospital (Lyon, France) undergoing major demolition. Three periods were scheduled in 2015: (A) Gutting of building and asbestos removal, (B) Demolition of floors, (C) Excavation and earthwork...
2018: PloS One
Valdemir Vicente da Silva Júnior, Laura Durão Ferreira, Lílian Rodrigues Alves, Adriane Borges Cabral, Paula Regina Luna de Araújo Jácome, Paulo Sérgio Ramos de Araújo, Ana Catarina de Souza Lopes, Maria Amélia Vieira Maciel
INTRODUCTION: Pseudomonas aeruginosa, an important pathogen globally, presents several resistance mechanisms. This study aimed to investigate the presence of bla GES in clinical isolates of Pseudomonas aeruginosa obtained from various clinical specimens from patients admitted to three different hospitals in Recife, Brazil. The Guiana extended spectrum beta-lactamase (GES) enzymes are responsible for conferring broad spectrum resistance to beta-lactam drugs, including the carbapenems. METHODS: A total of 100 carbapenem-resistant P...
November 2017: Revista da Sociedade Brasileira de Medicina Tropical
Baijayantimala Mishra, Vinaykumar Hallur, Bijayini Behera, C Preetam, Priti Nanda Mishra, Jyotirmayee Turuk, Prasanta Raghab Mohapatra
Tubercular lymphadenitis (TBLA) contributes to 30-40% of extrapulmonary TB cases in the immunocompetent individuals and 40-50% in people with HIV. Current diagnostic methods for TBLA like Gene-Xpert or PCR are costly and conventional methods like fine needle aspiration cytology, histopathology lack sensitivity and specificity. Culture which is considered as gold standard require high turnaround time. Loop mediated isothermal amplification (LAMP) assay has been developed as a novel technique for nucleic acid amplification and has shown promising results in the diagnosis of pulmonary tuberculosis...
January 2018: Indian Journal of Tuberculosis
Bansidhar Tarai, Dinesh Jain, Poonam Das, Sandeeep Budhiraja
The objective of this study was to show the differences between paired blood cultures (PBC) versus single blood cultures (SBC) in the microbiologic yield, the sensitivity to detect pathogens and the time to positivity (TTP). We performed a retrospective study examining 112,570 blood culture samples over a 5-year period from July 2011 to May 2016 in the BacT/ALERT® 3D automated blood culture system (bioMérieux, Marcy l'Etoile, France). Bacteria and yeasts were identified using the VITEK® 2 Compact system (bioMérieux, Marcy l'Etoile, France)...
January 11, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Bhargav Patel, Kinjal Patel, Anjali Shetty, Rajeev Soman, Camilla Rodrigues
Introduction: Antibiotic treatment of Urinary Tract Infections (UTI) is becoming increasingly difficult due to emergence of multi-drug resistant (ESBLs, AmpC, CRE) uropathogens. Fosfomycin is an old antibiotic that has evoked renewed interest with unique properties of not sharing any structural similarity and lack of cross-resistance with other antimicrobial agents. Our aim is to evaluate in-vitro activity of Fosfomycin against urinary tract Enterobacteriaceae. Material & Methods: The study period was March 2014 to September 2015...
September 2017: Journal of the Association of Physicians of India
Kanne Padmaja, Sukanya Sudhaharan, Lakshmi Vemu, Oruganti Sai Satish, Padmasri Chavali, Mamidi Neeraja
Background and Objectives: Infective endocarditis (IE) is a microbial infection of the endothelial surface of the cardiac-valves. Rapid diagnosis, effective treatment and prompt recognition of complications are essential, in order to improve the outcome. We retrospectively reviewed and determined the clinical characteristics, microbiological profile and management strategies of IE cases, changing microbial spectrum of pathogens and outcome in Native Valve Endocarditis (NVE) and Prosthetic Valve Endocarditis (PVE) cases...
October 2017: Iranian Journal of Microbiology
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