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https://www.readbyqxmd.com/read/28333363/evaluation-of-the-%C3%AE-carba%C3%A2-test-a-colorimetric-test-for-the-rapid-detection-of-carbapenemase-activity-in-gram-negative-bacilli
#1
Sandrine Bernabeu, Laurent Dortet, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated a novel colorimetric test (the β-CARBA™ test; Bio-Rad) to detect carbapenemase-producing Gram-negative bacilli from cultured colonies. Methods: The performance of the β-CARBA™ test was compared with that of the Carba NP test (or the CarbAcineto NP test) and RAPIDEC ® CARBA NP (bioMérieux) using a collection of 290 isolates with characterized β-lactamase content...
March 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28330891/evaluation-of-the-bionexia-legionella-test-including-the-impact-of-incubation-time-extension-for-the-detection-of-legionella-pneumophila-serogroup-1-antigen-in-urine
#2
Paul Badoux, Sjoerd M Euser, Jacob P Bruin, Patrick P G Mulder, Ed P F Yzerman
In this study, we compared the bioNexia (bioMérieux, Marcy-l'Étoile, France), a new immunochromatographic assay for the detection of Legionella pneumophila serogroup 1 in urine, with the BinaxNOW urinary antigen test (Alere, Waltham, Massachusetts, USA). After 15 minutes of incubation (in accordance with the manufactures instructions), the sensitivity and specificity were, respectively, 76.5% and 97.2% for the bioNexia test and 87.1% and 100% for the BinaxNOW test. After a prolonged incubation time of 60 minutes, the sensitivity and specificity increased to, respectively, 89...
March 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28330884/evaluation-of-biom%C3%A3-rieux-s-dissociated-vidas-lyme-igm-ii-lym-and-igg-ii-lyg-as-a-first-tier-diagnostic-assay-for-lyme-disease
#3
Claudia R Molins, Mark J Delorey, Adam Replogle, Christopher Sexton, Martin E Schriefer
The recommended laboratory diagnostic approach for Lyme disease is a standard two-tiered testing (STTT) algorithm where the first-tier is typically an enzyme immunoassay (EIA) that if positive or equivocal is reflexed to Western immunoblotting as the second-tier. bioMérieux manufacturers one of the most commonly used first-tier EIAs in the U.S., the combined IgM/IgG VIDAS (LYT). Recently, bioMérieux launched its dissociated first-tier tests, the VIDAS Lyme IgM II (LYM) and IgG II (LYG) EIAs, which use purified recombinant test antigens and a different algorithm than STTT...
March 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28318563/characterization-of-enterococcus-species-isolated-from-marine-recreational-waters-by-maldi-tof-ms-and-rapid-id-api%C3%A2-20-strep-system
#4
Ana Paula Guarnieri Christ, Solange Rodrigues Ramos, Rodrigo Cayô, Ana Cristina Gales, Elayse Maria Hachich, Maria Inês Zanoli Sato
MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMérieux's API® 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods...
March 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28283014/-fungemia-and-septic-arthritis-caused-by-saprochaete-capitata-in-a-patient-with-fanconi-aplastic-anemia-a-case-report
#5
Ömür Mustafa Parkan, Mustafa Altay Atalay, Ayşe Nedret Koç, Çiğdem Pala, Gonca Aydemir, Leylagül Kaynar
Saprochaete capitata (formerly known as Blastoschizomyces capitatus, Trichosporon capitatum, Geotrichum capitatum) is a rare but emerging yeast-like fungus. It is commonly found in environmental sources and can be isolated from skin, gastrointestinal system and respiratory tract of healthy individuals as well. It mainly infects patients with hematological malignancies such as acute myeloid leukemia (AML), especially in the presence of neutropenia; and mortality rates are high in those patients. Although the data about the in vitro antifungal susceptibility are limited, it is being reported that amphotericin B and voriconazole are more effective on S...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28283005/-comparison-of-maldi-tof-and-16s-rrna-methods-in-identification-of-viridans-group-streptococci
#6
Serap Süzük Yıldız, Banu Kaşkatepe, Salih Altınok, Mustafa Çetin, Alper Karagöz, Sümeyra Savaş
Accurate identification of viridans group streptococci (VGS) frequently encountered as a causative agent of infective endocarditis is always a challenge for the clinical microbiology laboratory. Clinical microbiology laboratories generally use semi automatic/full automatic systems, molecular methods and also conventional methods for the identification of these bacteria. There are recent published studies that have used MALDI-TOF (Matrix Assisted Laser Ionization Mass Spectrometry-Time of Flight) systems in the identification of VGS...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28279857/performance-of-two-blood-culture-systems-to-detect-anaerobic-bacteria-is-there-any-difference
#7
Manica Mueller-Premru, Samo Jeverica, Lea Papst, Elisabeth Nagy
We studied the performance characteristics of two blood culture (BC) bottles/systems, (i) BacT/ALERT-FN Plus/3D (bioMérieux, Marcy l'Étoile, France) and (ii) BACTEC-Lytic/9000 (Becton Dickinson, Sparks, USA) for detection of growth and time-to-positivity (TTP) against a balanced and diverse collection of anaerobic bacterial strains (n = 48) that included reference strains (n = 19) and clinical isolates (n = 29) of 32 species (15 Gram-negative and 17 Gram-positive) was tested. Standard suspension of bacteria was inoculated to each bottle in duplicates and incubated in the corresponding system...
March 6, 2017: Anaerobe
https://www.readbyqxmd.com/read/28279212/rna-extraction-method-is-crucial-for-human-papillomavirus-e6-e7-oncogenes-detection
#8
Nerea Fontecha, Maria Carmen Nieto, Daniel Andía, Ramón Cisterna, Miren Basaras
BACKGROUND: Human papillomavirus (HPV) DNA testing plays a main role in the management of cervical cancer, however to improve the specificity in cervical screening, there is a need to develop and validate different approaches that can identify women at risk for progressive disease. Nowadays, mRNA expression of viral E6 and E7 HPV oncogenes stands up as a potential biomarker to improve cervical screening. We aimed to validate a method for RNA extraction, detect HPV mRNA expression and, assess the relationship between E6/E7 mRNA expression and pathology of patients' lesions and progression...
March 9, 2017: Virology Journal
https://www.readbyqxmd.com/read/28273966/stenotrophomonas-maltophilia-an-emerging-pathogen-in-paediatric-population
#9
Charu Nayyar, Preeti Thakur, Vibhor Tak, Karnika Saigal
INTRODUCTION: Stenotrophomonas maltophilia (formerly Pseudomonas maltophilia/Xanthomonas maltophilia), a Gram- negative, non-fermenting bacillus, is being increasingly recognized as a threatening nosocomial pathogen, associated with significant mortality. AIM: To determine the prevalence of infection, antimicrobial susceptibility pattern and clinical outcome of S. maltophilia in a paediatric population. MATERIALS AND METHODS: This was a retrospective study conducted over a period of eight months, i...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28264610/corynebacterium-species-causing-breast-abscesses-among-patients-attending-a-tertiary-care-hospital-in-chennai-south-india
#10
Indira Poojary, Ann Kurian, Jayalekshmi V A, Debora Devapriya J, Thirunarayan M A
BACKGROUND: Corynebacterium species other than Corynebacterium diphtheriae were mostly considered contaminants in the past, but there are reports of their association with wide variety of human infections lately. In this study, we look into Corynebacterium species isolated from breast abscess patients and assess their antimicrobial susceptibility pattern and treatment outcomes. MATERIAL AND METHODS: Pus samples from suspected breast abscess cases were examined from October 2014 to September 2015...
March 6, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28260586/molecular-epidemiology-of-pseudomonas-aeruginosa-bloodstream-infection-isolates-in-a-non-outbreak-setting
#11
Kate L McCarthy, Tim J Kidd, David L Paterson
PURPOSE: The molecular epidemiology of Pseudomonas aeruginosa bloodstream infection (BSI) isolates has received limited attention. This study aims to characterize the molecular relationship of P. aeruginosa BSI isolates in the non-outbreak setting at a single tertiary healthcare facility. METHODOLOGY:   P. aeruginosa BSI isolates from patients who were admitted to the Royal Brisbane and Women's Hospital over a 13 month period from November 2009 were identified retrospectively from the Pathology Queensland Clinical and Scientific Information System...
March 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28243540/retrospective-observation-of-drug-susceptibility-of-candida-strains-in-the-years-1999-2004-and-2015
#12
Cecylia Łukaszuk, Elżbieta Krajewska-Kułak, Wojciech Kułak
INTRODUCTION: There is much literature devoted to the problem of drug resistance and decreased susceptibility of fungi to commonly used antifungals. AIM: To analyze drug susceptibility of Candida albicans and non-Candida albicans strains isolated from the hands of people without any symptoms of disease over a 16-year period. MATERIALS AND METHODS: The study included a total of 1,274 Candida-type strains isolated from the hands of people without any symptoms of disease, including: in 1999, 432 strains; in 2004, 368; and in 2015, 454 strains...
2017: PeerJ
https://www.readbyqxmd.com/read/28241707/-the-analysis-of-pathogens-distribution-and-drug-resistance-of-bacteria-in-sputum-samples-of-pneumoconiosis-patients-combined-with-lower-respiratory-tract-infection
#13
Y Xin, N Zhang
Objective: To guide the rational use of antibiotic drugs in clinical practice for doctors by the analysis of pathogens distribution, transition and drug resistance of bacteria in sputum samples of pneumoconiosis patients combined with lower respiratory tract infection. Methods: 2 199 qualified sputum samples from 214 pneumoconiosis patients collected from January 2008 to December 2012 were examined by bacterium or fungus education. BioMerieux API and Eastern Cowin application software were used to do bacterial identification, and K-B method recommended by the NCCLS was used to take drug sensitivity tests...
January 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28220547/identification-and-molecular-epidemiology-of-dermatophyte-isolates-by-repetitive-sequence-pcr-based-dna-fingerprinting-using-the-diversilab-system-in-turkey
#14
A Nedret Koc, Mustafa A Atalay, Melek Inci, Fatma M Sariguzel, Hafize Sav
Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux)...
February 21, 2017: Mycoses
https://www.readbyqxmd.com/read/28160729/comparison-of-emag%C3%A2-versus-nuclisens-%C3%A2-easymag-%C3%A2-performance-on-clinical-specimens
#15
Magali Garcia, Céline Chessa, Anne Bourgoin, Geneviève Giraudeau, Chloé Plouzeau, Gérard Agius, Nicolas Lévêque, Agnès Beby-Defaux
BACKGROUND: eMAG™ (bioMerieux) is a new nucleic acid extraction platform based on magnetic silica technology, like its predecessor, NucliSENS(®) easyMAG(®) (bioMerieux). Using the same reagents and disposables, eMAG™ adds further automation, allowing simultaneous extraction of 48 samples directly from primary tubes, and distribution of nucleic acid extracts on PCR strips or in tubes at the end of the extraction process. OBJECTIVE: To compare the performance of eMAG™ and easyMAG(®) on various clinical specimens...
March 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28159668/performance-analysis-of-two-immunochromatographic-assays-for-the-diagnosis-of-rotavirus-infection
#16
S De Grazia, F Bonura, A Pepe, S Li Muli, V Cappa, A Collura, D M Terranova, N Urone, F Di Bernardo, D Matranga, G M Giammanco
Group A rotaviruses (RVAs) are the primary cause of acute gastroenteritis (AGE) in young children worldwide. Several commercial tests including latex agglutination, enzyme-linked assays (ELISA) and immunochromatographic tests (ICT) have been developed for the diagnosis of RVA infection. In the present study, the performance of two commercially available one-step chromatographic immunoassays, CerTest Rotavirus+Adenovirus (Biotec S.L, Zaragoza, Spain) and Vikia Rota-Adeno (bioMerieux SA, Lyon, France) were retrospectively evaluated using Real-time PCR as reference test...
May 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28154553/identification-and-antifungal-susceptibility-profiles-of-candida-nivariensis-and-candida-bracarensis-in-a-multi-center-chinese-collection-of-yeasts
#17
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Shu-Ying Yu, Xin Fan, Fanrong Kong, Ying-Chun Xu
Candida nivariensis and C. bracarensis are two emerging cryptic species within the C. glabrata complex. Thirteen of these isolates from 10 hospitals in China were studied for their species identification and antifungal susceptibilities. Phenotypic and molecular [rDNA ITS sequencing, D1/D2 sequencing and ITS sequencer-based capillary gel electrophoresis (SCGE)] and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS identification methods were compared for their performance in species identification...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28150479/the-intestinal-mycobiota-a-year-of-observation-about-the-incidence-of-yeast-s-isolation-in-fecal-samples
#18
Lucia Barcella, Santa B Rogolino, Angelo P Barbaro
BACKGROUND: Yeast's presence, especially Candida's species, in the intestinal tract is now considered as part of the normal microbial human flora; however, fungal colonization of the colon may induce to fungal infections, becoming a risk not only in immunocompromised patients but also in normalcompetent subjects. The purpose of this epidemiological study was to evaluate the real incidence of yeasts into the fecal samples both from healthy outpatients and from inpatients, except those from wards concerning immunosuppressive and pediatrics pathologies...
February 1, 2017: Minerva Gastroenterologica e Dietologica
https://www.readbyqxmd.com/read/28131414/diversity-of-enterococcus-faecalis-genotypes-from-multiple-oral-sites-associated-with-endodontic-failure-using-repetitive-sequence-based-polymerase-chain-reaction-and-arbitrarily-primed-polymerase-chain-reaction
#19
Maraísa G Delboni, Brenda P F A Gomes, Priscila A Francisco, Fabrício B Teixeira, David Drake
INTRODUCTION: The aim of this study was to evaluate the diversity and similarity of Enterococcus faecalis genotype isolates from multiple oral sites using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction (AP-PCR). METHODS: Forty-two endodontically treated teeth with apical periodontitis were selected. A total of 126 microbial samples were collected from 3 different sites (saliva, pulp chamber, and root canals, all n = 42) during the nonsurgical retreatment procedures...
March 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#20
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
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