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https://www.readbyqxmd.com/read/29353064/multiplexed-isothermal-nucleic-acid-amplification
#1
Olena Mayboroda, Ioanis Katakis, Ciara K O'Sullivan
Multiplexed isothermal amplification and detection of nucleic acid sequences and biomarkers is of increasing importance in diverse areas including advanced diagnostics, food quality control and environmental monitoring. Whilst there are several very elegant isothermal amplification approaches, multiplexed amplification remains a challenge, requiring careful experimental design and optimisation, from judicious primer design in order to avoid the formation of primer dimers and non-specific amplification, applied temperature as well as the ratio and concentration of primers...
January 15, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29344968/a-novel-nested-polymerase-chain-reaction-assay-targeting-plasmodium-mitochondrial-dna-in-field-collected-anopheles-mosquitoes
#2
M Calzetta, E Perugini, G Seixas, C A Sousa, W M Guelbeogo, N Sagnon, A Della Torre, J Pinto, M Pombi, E Mancini
Sensitive techniques for the detection of Plasmodium (Aconoidasida: Plasmodiidae) sporozoites in field-collected malaria vectors are essential for the correct assessment of risk for malaria transmission. A real-time polymerase chain reaction (RT-PCR) protocol targeting Plasmodium mtDNA proved to be much more sensitive in detecting sporozoites in mosquitoes than the widely used enzyme-linked immunosorbent assay targeting Plasmodium circumsporozoite protein (CSP-ELISA). However, because of the relatively high costs associated with equipment and reagents, RT-PCRs are mostly used to assess the outcomes of experimental infections in the frame of research experiments, rather than in routine monitoring of mosquito infection in the field...
January 18, 2018: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/29343880/novel-application-of-loop-mediated-isothermal-amplification-for-rapid-detection-of-gene-translocation
#3
Ibu Matsuzaki, Hideto Iguchi, Yurina Mikasa, Hiromu Morishita, Katsuya Okuda, Keita Nakaguchi, Yuki Mori, Yoshifumi Iwahashi, Kenji Warigaya, Masakazu Fujimoto, Fumiyoshi Kojima, Shin-Ichi Murata
Identification of fusion genes in cancer is essential for pathological diagnosis and clinical therapy. Although methods for detection of fusion genes, such as fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction (PCR), have been developed in last two decades, these methods are not ideal for detection of these genetic alterations owing to their high cost and time-consuming procedures. In this study, we developed novel application for detection of gene translocations using loop-mediated isothermal amplification (LAMP)...
December 26, 2017: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29336956/reverse-transcription-loop-mediated-isothermal-amplification-for-species-specific-detection-of-tomato-chlorotic-spot-orthotospovirus
#4
Xuelian Sui, Shouan Zhang, Zujian Wu, Kai-Shu Ling
Tomato chlorotic spot orthotospovirus (TCSV) is an emerging orthotospovirus that can cause severe disease on tomato plants. There are at least four orthotospoviruses infecting tomato, and mixed infection of two or more orthotospoviruses in a single tomato plant is quite common in the field. With similarity in the symptomatology and cross serological reactivity among tomato-infecting orthotospoviruses, especially between TCSV and groundnut ringspot orthotospovirus (GRSV), the current serological tests could not achieve definite and accurate species-specific determination in disease diagnosis...
January 11, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29335461/pixelated-spatial-gene-expression-analysis-from-tissue
#5
A Ganguli, A Ornob, N Spegazzini, Y Liu, G Damhorst, T Ghonge, B Thornton, C J Konopka, W Dobrucki, S E Clare, R Bhargava, A M Smith, F Kosari, R Bashir
Here, we present a technique that performs on-chip picoliter real-time reverse transcriptase loop mediated isothermal amplification (RT-LAMP) reactions on a histological tissue section without any analyte purification while preserving the native spatial location of the nucleic acid molecules. We demonstrate this method by amplifying TOP2A messenger RNA (mRNA) in a prostate cancer xenograft with 100 µm spatial resolution and by visualizing the variation in threshold time of amplification across the tissue...
January 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29332654/evaluation-of-loop-mediated-isothermal-amplification-lamp-assay-in-the-diagnosis-of-tubercular-lymphadenitis-a-pilot-study
#6
Baijayantimala Mishra, Vinaykumar Hallur, Bijayini Behera, C Preetam, Priti Nanda Mishra, Jyotirmayee Turuk, Prasanta Raghab Mohapatra
Tubercular lymphadenitis (TBLA) contributes to 30-40% of extrapulmonary TB cases in the immunocompetent individuals and 40-50% in people with HIV. Current diagnostic methods for TBLA like Gene-Xpert or PCR are costly and conventional methods like fine needle aspiration cytology, histopathology lack sensitivity and specificity. Culture which is considered as gold standard require high turnaround time. Loop mediated isothermal amplification (LAMP) assay has been developed as a novel technique for nucleic acid amplification and has shown promising results in the diagnosis of pulmonary tuberculosis...
January 2018: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/29330674/advances-in-serological-imaging-techniques-and-molecular-diagnosis-of-toxoplasma-gondii-infection
#7
REVIEW
Ali Rostami, Panagiotis Karanis, Shirzad Fallahi
BACKGROUND: Toxoplasmosis is worldwide distributed zoonotic infection disease with medical importance in immunocompromised patients, pregnant women and congenitally infected newborns. Having basic information on the traditional and new developed methods is essential for general physicians and infectious disease specialists for choosing a suitable diagnostic approach for rapid and accurate diagnosis of the disease and, consequently, timely and effective treatment. METHODS: We conducted English literature searches in PubMed from 1989 to 2016 using relevant keywords and summarized the recent advances in diagnosis of toxoplasmosis...
January 12, 2018: Infection
https://www.readbyqxmd.com/read/29329857/meat-species-identification-using-dna-luminol-interaction-and-their-slow-diffusion-onto-the-biochip-surface
#8
Nura Fazira Noor Azam, Sharmili Roy, Syazana Abdullah Lim, Minhaz Uddin Ahmed
Recently, there has been a growing concern of consumers on the authenticity of food ingredients including adulteration with porcine and/or its derivatives. Therefore, this work reports on the development of a novel, simple, sensitive and rapid luminol-based electrochemiluminescence (ECL) technique for Sus scrofa (Porcine) DNA detection. Porcine DNA was firstly amplified using loop-mediated isothermal amplification (LAMP) method and subsequently added to luminol solution for further ECL analysis for quantification...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329617/dna-detection-of-trypanosoma-evansi-diagnostic-validity-of-a-new-assay-based-on-loop-mediated-isothermal-amplification-lamp
#9
Qunbo Tong, Rui Chen, Qingming Kong, Julie Goossens, Magdalena Radwanska, Di Lou, Jianzu Ding, Bin Zheng, Yixiu Fu, Tianping Wang, Magez Stefan, Shaohong Lu
Trypanosoma evansi (T. evansi) is the most widely spread pathogenic trypanosome in the world. The control of trypanosomiasis depends on accurate diagnosis and effective treatment. Focusing on the presence of T. evansi in Asia, we developed a detection assay based on tracing phosphate ions (Pi) generated during LAMP targeting the variant surface glycoprotein (VSG) gene of Rode Trypanozoon antigenic type 1.2 (RoTat 1.2 VSG). The diagnostic potential as well as the use of the assay as a test-of-cure method after berenil treatment, was assessed in mice at different time points of infection...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29325863/future-high-flux-assays-on-pathogenic-virulent-and-toxic-genetics-from-various-pathogens
#10
REVIEW
Yanmei Li, Caiying Bai, Ling Yang, Jie Fu, Muxia Yan, Dingqiang Chen, Li Zhang
Toxins, encoding by virulence factors, are significant cause of food-borne illnesses and death in the worldwide. Loop-mediated isothermal amplification (LAMP) is one of the widely used methodologies because of the high sensitivity, specificity and rapidity. Nowadays, LAMP has been regarded as an innovative gene amplification technology and emerged as an alternative to PCR-based methodologies in identification of the pathogenic virulent and toxic genetics. The high sensitivity of LAMP enables detection of the pathogens in sample materials even without time consuming and sample preparation...
January 8, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29323478/simultaneous-detection-of-antibiotic-resistance-genes-on-paper-based-chip-using-ru-phen-2dppz-2-turn-on-fluorescence-probe
#11
Bofan Li, Xiaoming Zhou, Hongxing Liu, Huaping Deng, Ru Huang, Da Xing
Antibiotic resistance, the ability of some bacteria to resist antibiotic drug, has been a major global health burden due to the extensive use of antibiotic agents. Antibiotic resistance is encoded via respective genes, hence the specific detection of these genes is necessary for diagnostic and treat of antibiotic resistance cases. Conventional methods for monitoring antibiotic resistance genes require sample to be transported to a central laboratory for tedious and sophisticated tests, which is grueling and time-consuming...
January 11, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29322764/rapid-veterinary-diagnosis-of-bovine-reproductive-infectious-diseases-from-semen-using-paper-origami-dna-microfluidics
#12
Zhugen Yang, Gaolian Xu, Julien Reboud, Syed Atif Ali, Gurpreet Kaur, John McGiven, Nongthombam Boby, Praveen Gupta, Pallab Chaudhuri, Jonathan Mark Cooper
The health and well-being of cattle is a significant concern for global agricultural output. In dairy production within low and middle income countries (LMICs), there is a significant biosensing challenge in detecting sexually transmitted infection (STI) pathogens during animal husbandry, due in part to difficulties associated with the limited infrastructure for veterinary medicine. Here we demonstrate low-cost, multiplexed and sample-to-answer paper-origami tests for the detection of three bovine infectious reproductive diseases in semen samples, collected at a test site in rural India...
January 11, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29317875/diagnostic-accuracy-of-loop-mediated-isothermal-amplification-assay-as-a-field-molecular-tool-for-rapid-mass-screening-of-old-world-leishmania-infections-in-sand-flies-and-in-vitro-culture
#13
Mehdi Ghodrati, Adel Spotin, Teimour Hazratian, Mahmoud Mahami-Oskouei, Ali Bordbar, Sahar Ebrahimi, Shirzad Fallahi, Parviz Parvizi
Background: We employed a highly sensitive loop-mediated isothermal amplification (LAMP) by targeting 18S rRNA gene to identify the rapid mass screening of Leishmania infections in captured sand flies of southwest Iran and In vitro culture. Methods: One hundred fifty sand flies were collected from 11 sites adjacent to Iraqi's borders in southern parts of Khuzestan Province by using sticky sheets of paper and CDC miniature light traps during late May 2014 to Nov 2015...
October 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/29310262/nano-biotinylated-liposome-based-immunoassay-for-the-ultrasensitive-detection-of-protein-biomarker-in-urine
#14
Peng Liu, Xueen Fang, Hongmei Cao, Mingli Gu, Jilie Kong, Anmei Deng
With the development of proteomics and the continuous discovery of biomarkers of trace proteins, it is important to accurately quantify low abundance protein, especially in urine for clinical diagnostics. In this paper, we reported a novel nano-biotinylated liposome-based immuno-loop-mediated isothermal amplification (LI-LAMP) for the ultrasensitive detection of REG1A (a biomarker for pancreatic ductal adenocarcinoma (PDAC) in urine) with high specificity. The detection range was 1µg/mL to 1fg/mL, with a detection limit of 1fg/mL, and no cross-reactivity was observed to occur in this assay...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29297912/temperature-change-rate-actuated-bubble-mixing-for-homogeneous-rehydration-of-dry-pre-stored-reagents-in-centrifugal-microfluidics
#15
S Hin, N Paust, M Keller, M Rombach, O Strohmeier, R Zengerle, K Mitsakakis
In centrifugal microfluidics, dead volumes in valves downstream of mixing chambers can hardly be avoided. These dead volumes are excluded from mixing processes and hence cause a concentration gradient. Here we present a new bubble mixing concept which avoids such dead volumes. The mixing concept employs heating to create a temperature change rate (TCR) induced overpressure in the air volume downstream of mixing chambers. The main feature is an air vent with a high fluidic resistance, representing a low pass filter with respect to pressure changes...
January 3, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29291217/rapid-detection-of-tomato-leaf-curl-bengaluru-virus-through-loop-mediated-isothermal-amplification-assay
#16
R Arutselvan, M Krishna Reddy, T Makeshkumar
A loop-mediated isothermal amplification (LAMP) technique was employed to develop a simple and rapid method for the detection of tomato leaf curl Bangalore virus (ToLCBaV) in diseased plants of tomato (Solanum lycopersicum). Six sets of primers were designed for LAMP technique targeting the conserved AC1 region and successfully detected ToLCBaV. No reaction was detected in the tissues of healthy plants by either the LAMP or the polymerase chain reaction (PCR). The LAMP products can be visualized by presence or absence of turbidity and staining (0...
September 2017: Virusdisease
https://www.readbyqxmd.com/read/29286383/visual-detection-of-multiple-nucleic-acids-in-a-capillary-array
#17
Jianwei Chen, Ning Shao, Jiaying Hu, Rong Li, Yuanshou Zhu, Dabing Zhang, Shujuan Guo, Junhou Hui, Peng Liu, Litao Yang, Sheng-Ce Tao
Multi-target, short time, and resource-affordable methodologies for the detection of multiple nucleic acids in a single, easy to operate test are urgently needed in disease diagnosis, microbial monitoring, genetically modified organism (GMO) detection, and forensic analysis. We have previously described the platform called CALM (Capillary Array-based Loop-mediated isothermal amplification for Multiplex visual detection of nucleic acids). Herein, we describe improved fabrication and performance processes for this platform...
November 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29279568/rapid-detection-of-candida-albicans-in-oral-exfoliative-cytology-samples-by-loop-mediated-isothermal-amplification
#18
Hiroyasu Noguchi, Takashi Iwase, Daisuke Omagari, Masatake Asano, Ryota Nakamura, Kosuke Ueki, Keiji Shinozuka, Tadayoshi Kaneko, Morio Tonogi, Hiderou Ohki
Loop-mediated isothermal amplification (LAMP) rapidly amplifies DNA under isothermal conditions. The aim of this study was to detect Candida albicans and compare the positivity rate in the LAMP reaction with that of conventional methods for oral exfoliative cytology (EC) samples. Sixty-eight EC samples from 53 patients were subjected to LAMP analysis. These patients had been clinically diagnosed with leukoplakia, squamous cell carcinoma, oral lichen planus (OLP), stomatitis, oral candidiasis, and other malignancies...
2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29278789/portable-platform-for-rapid-in-field-identification-of-human-fecal-pollution-in-water
#19
Yu Sherry Jiang, Timothy E Riedel, Jessica A Popoola, Barrett R Morrow, Sheng Cai, Andrew D Ellington, Sanchita Bhadra
Human fecal contamination of water is a public health risk. However, inadequate testing solutions frustrate timely, actionable monitoring. Bacterial culture-based methods are simple but typically cannot distinguish fecal host source. PCR assays can identify host sources but require expertise and infrastructure. To bridge this gap we have developed a field-ready nucleic acid diagnostic platform and rapid sample preparation methods that enable on-site identification of human fecal contamination within 80 min of sampling...
December 13, 2017: Water Research
https://www.readbyqxmd.com/read/29277714/loop-mediated-isothermal-amplification-assay-for-the-rapid-and-visual-detection-of-the-novel-porcine-circovirus-3
#20
Yu-Ri Park, Hye-Ryung Kim, Seong-Hee Kim, Kyoung-Ki Lee, Young S Lyoo, Sang-Geon Yeo, Choi-Kyu Park
A loop-mediated isothermal amplification (LAMP) assay using hydroxynaphthol blue was developed for the rapid and visual detection of the capsid gene of porcine circovirus 3 (PCV3). The amplification could be completed in 40 min at 62 °C, and the results could be visually detected by the naked eye. The assay specifically amplified PCV3 DNA and not other porcine viral nucleic acids. The limit of detection of the assay was 50 PCV3 DNA copies, which was comparable to that of the real-time polymerase chain reaction (qPCR) and lower than that of conventional PCR...
December 22, 2017: Journal of Virological Methods
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