keyword
https://read.qxmd.com/read/38534259/customizable-nichrome-wire-heaters-for-molecular-diagnostic-applications
#1
JOURNAL ARTICLE
Juhee Lim, Won Han, Le Tran Huy Thang, Yong Wook Lee, Joong Ho Shin
Accurate sample heating is vital for nucleic acid extraction and amplification, requiring a sophisticated thermal cycling process in nucleic acid detection. Traditional molecular detection systems with heating capability are bulky, expensive, and primarily designed for lab settings. Consequently, their use is limited where lab systems are unavailable. This study introduces a technique for performing the heating process required in molecular diagnostics applicable for point-of-care testing (POCT), by presenting a method for crafting customized heaters using freely patterned nichrome (NiCr) wire...
March 20, 2024: Biosensors
https://read.qxmd.com/read/38534246/a-point-of-care-nucleic-acid-quantification-method-by-counting-light-spots-formed-by-lamp-amplicons-on-a-paper-membrane
#2
JOURNAL ARTICLE
Yanju Chen, Yuanyuan Zhu, Cheng Peng, Xiaofu Wang, Jian Wu, Huan Chen, Junfeng Xu
Nucleic acid quantification, allowing us to accurately know the copy number of target nucleic acids, is significant for diagnosis, food safety, agricultural production, and environmental protection. However, current digital quantification methods require expensive instruments or complicated microfluidic chips, making it difficult to popularize in the point-of-care detection. Paper is an inexpensive and readily available material. In this study, we propose a simple and cost-effective paper membrane-based digital loop-mediated isothermal amplification (LAMP) method for nucleic acid quantification...
March 10, 2024: Biosensors
https://read.qxmd.com/read/38534235/low-cost-arduino-reverse-transcriptase-loop-mediated-isothermal-amplification-rt-lamp-for-sensitive-nucleic-acid-detection
#3
JOURNAL ARTICLE
Bruno Dias Camargo, Mateus Cassaboni Stracke, Heloisa Bruna Soligo Sanchuki, Viviane Klassen de Oliveira, Hellen Cristina Ancelmo, Dayanne Mozaner Bordin, Fabricio Klerynton Marchini, Emilson Ribeiro Viana, Lucas Blanes
This work presents a low-cost transcription loop-mediated isothermal amplification (RT-LAMP) instrument for nucleic acid detection, employing an Arduino Nano microcontroller. The cooling system includes customized printed circuit boards (PCBs) that serve as electrical resistors and incorporate fans. An aluminum block is designed to accommodate eight vials. The system also includes two PCB heaters-one for sample heating and the other for vial lid heating to prevent condensation. The color detection system comprises a TCS3200 color 8-sensor array coupled to one side of the aluminum heater body and a white 8-LED array coupled to the other side, controlled by two Multiplexer/Demultiplexer devices...
February 29, 2024: Biosensors
https://read.qxmd.com/read/38534170/use-of-the-23s-rrna-gene-as-a-target-template-in-the-universal-loop-mediated-isothermal-amplification-lamp-of-genomic-dna-from-phytoplasmas
#4
JOURNAL ARTICLE
Mako Akahori, Akio Miyazaki, Hiroaki Koinuma, Ryosuke Tokuda, Nozomu Iwabuchi, Yugo Kitazawa, Kensaku Maejima, Shigetou Namba, Yasuyuki Yamaji
Plant-pathogenic bacteria cause numerous diseases in host plants and can result in serious damage. Timely and accurate diagnostic techniques are, therefore, crucial. While advances in molecular techniques have led to diagnostic systems able to distinguish known plant pathogens at the species or strain level, systems covering larger categories are mostly lacking. In this study, a specific and universal LAMP-based diagnostic system was developed for phytoplasmas, a large group of insect-borne plant-pathogenic bacteria that cause significant agricultural losses worldwide...
March 27, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38533815/a-novel-loop-mediated-isothermal-amplification-lamp-assay-to-diagnose-feline-panleukopenia
#5
JOURNAL ARTICLE
R A Bakde, R L Rathish, L John, P M Deepa, K Vijayakumar
Protoparvovirus carnivoran1 , known commonly as feline panleukopenia virus (FPV) is a highly contagious and environmentally stable parvovirus of domestic as well as wild felids. A rapid and robust diagnostic tool will aid in implementing prompt treatment and control measures. A loop-mediated isothermal amplification (LAMP) as a point-of-care diagnostic tool for diagnosing feline panleukopenia was standardised using faecal samples of cats. The assay will reduce the cost and time required to diagnose feline panleukopenia...
March 26, 2024: Journal of the South African Veterinary Association
https://read.qxmd.com/read/38530068/bridging-the-gap-r-loop-mediated-genomic-instability-and-its-implications-in-neurological-diseases
#6
REVIEW
Katherine R Westover, Peng Jin, Bing Yao
R-loops, intricate three-stranded structures formed by RNA-DNA hybrids and an exposed non-template DNA strand, are fundamental to various biological phenomena. They carry out essential and contrasting functions within cellular mechanisms, underlining their critical role in maintaining cellular homeostasis. The specific cellular context that dictates R-loop formation determines their function, particularly emphasizing the necessity for their meticulous genomic regulation. Notably, the aberrant formation or misregulation of R-loops is implicated in numerous neurological disorders...
March 26, 2024: Epigenomics
https://read.qxmd.com/read/38528151/structural-basis-of-the-histone-ubiquitination-read-write-mechanism-of-rybp-prc1
#7
JOURNAL ARTICLE
Maria Ciapponi, Elena Karlukova, Sven Schkölziger, Christian Benda, Jürg Müller
Histone H2A monoubiquitination (H2Aub1) by the PRC1 subunit RING1B entails a positive feedback loop, mediated by the RING1B-interacting protein RYBP. We uncover that human RYBP-PRC1 binds unmodified nucleosomes via RING1B but H2Aub1-modified nucleosomes via RYBP. RYBP interactions with both ubiquitin and the nucleosome acidic patch create the high binding affinity that favors RYBP- over RING1B-directed PRC1 binding to H2Aub1-modified nucleosomes; this enables RING1B to monoubiquitinate H2A in neighboring unmodified nucleosomes...
March 25, 2024: Nature Structural & Molecular Biology
https://read.qxmd.com/read/38527171/sensitive-and-specific-loop-mediated-isothermal-amplification-assays-for-detection-of-salmonella-ctx-m-1-group-genes-mph-a-and-ermb-in-stool-and-blood-samples-based-on-orange-to-green-visible-dye
#8
JOURNAL ARTICLE
Jianbo Feng, Ya Huang, Huixin Chen, Shujin Xie, Congzhu Yang, Weixin Zheng, Jinglin Su, Wentao Zheng, Jiajie Mo, Fei Lv
Salmonella is a globally prevalent foodborne bacterium, and ceftriaxone and azithromycin have been regarded as drugs of choice for treating Salmonella infections, particularly in children. With the growing incidence of ceftriaxone and azithromycin resistance in Salmonella , there is an urgent requirement for a rapid and dependable gene testing approach to enhance the efficacy of treating Salmonella infections. Utilizing the orange to green visible dye approach, this study developed loop-mediated isothermal amplification (LAMP) assays for the sensitive and specific detection of Salmonella , ceftriaxone and azithromycin resistance genes (including CTX-M-1 group, mph(A) , and ermB genes) in stool and blood samples...
March 22, 2024: Foodborne Pathogens and Disease
https://read.qxmd.com/read/38523772/design-and-evaluation-of-loop-mediated-isothermal-amplification-for-rapid-detection-of-enterocytozoon-bieneusi
#9
JOURNAL ARTICLE
Fatemeh Mahdavi, Hamed Mirjalali, Maryam Niyyati, Seyyed Javad Seyyed Tabaei, Amir Shamloo, Hamid Asadzadeh Aghdaei
Enterocytozoon bieneusi is one of the most prevalent microsporidia species, responsible for more than 90% of human and animal microsporidiosis. Microsporidia species, particularly E. bieneusi, are frequently reported from waterborne and foodborne outbreaks. Therefore, early detection is crucial in clinics and outbreak investigations. This study aimed to design a loop-mediated isothermal amplification (LAMP) for rapid detection of E. bieneusi. Total DNA was extracted from 30 E. bieneusi -positive samples, which had been confirmed with nested PCR...
June 2024: Food and waterborne parasitology
https://read.qxmd.com/read/38518558/a-handheld-isothermal-fluorescence-detector-for-duplex-visualization-of-aquatic-pathogens-via-enhanced-one-pot-lamp-pfago-assay
#10
JOURNAL ARTICLE
Feibiao Pang, Tao Zhang, Fengyi Dai, Kaizheng Wang, Tianjiao Jiao, Zuoying Zhang, Liyi Zhang, Mingli Liu, Peng Hu, Jinzhao Song
The expansion of large-scale aquaculture has exacerbated the challenge of aquatic diseases, resulting in substantial economic losses annually. Currently, traditional laboratory-based diagnostic methods are time-consuming and costly, hindering on-site testing for individual farmers. We address this issue by developing a state-of-the-art handheld isothermal nucleic acid amplification device (WeD-1) capable of fluorescence tracking of reactions and integrating it with an enhanced one-pot Prokaryotic Argonaute based nucleic acid detection method, enabling duplex visual detection of aquatic pathogens...
March 8, 2024: Biosensors & Bioelectronics
https://read.qxmd.com/read/38518150/application-of-thermal-cycling-amplification-chip-method-in-the-detection-of-respiratory-machine-associated-pneumonia-pathogens
#11
JOURNAL ARTICLE
Jiajia Liu, Shuai Zhao, Haiyang Yan, Suli Jin, Jingna Sun, Lidan Xing, Xiaoye Liu, Lisha Wang, Jing Chen
OBJECTIVE: Investigating the application effectiveness of using loop-mediated isothermal amplification (LAMP) on a microfluidic chip to detect the pathogens associated with ventilator-associated pneumonia (VAP). METHODS: Eighty samples of bronchoalveolar lavage fluid from patients with ventilator-associated pneumonia (VAP) were collected at The First Hospital of Hebei Medical University from July 2022 to July 2023. The bacterial culture technique and the LAMP method were used to detect the nucleic acid of the pathogens in the patient samples...
March 22, 2024: Alternative Therapies in Health and Medicine
https://read.qxmd.com/read/38513919/development-of-a-closed-tube-calcein-based-loop-mediated-isothermal-amplification-assay-to-detect-salmonella-spp-in-raw-meat-samples
#12
JOURNAL ARTICLE
Khristine B Balaga, Rance Derrick N Pavon, Alyzza Marie B Calayag, Christine Aubrey C Justo, Davin Edric V Adao, Windell L Rivera
Foodborne pathogens compromise food safety and public health, and Salmonella spp. are among the major pathogenic bacteria that cause outbreaks worldwide. Proper surveillance through timely and cost-effective detection methods across the food animal production chain is crucial to prevent Salmonella outbreaks and agricultural losses. Traditional culture methods are labor- and resource-intensive, with lengthy turnaround times. Meanwhile, conventional molecular tools, such as PCR and qPCR, are expensive and require technical skills and equipment...
March 19, 2024: Journal of Microbiological Methods
https://read.qxmd.com/read/38512825/evaluation-of-a-rapid-loop-mediated-isothermal-amplification-lamp-test-for-the-laboratory-diagnosis-of-sexually-transmitted-infections
#13
JOURNAL ARTICLE
Martina Brandolini, Laura Grumiro, Patrizia Farabegoli, Giorgio Dirani, Silvia Zannoli, Irene Zaghi, Massimiliano Guerra, Francesca Taddei, Giulia Gatti, Anna Marzucco, Maria Sofia Montanari, Alessandra Mistral De Pascali, Simona Semprini, Monica Cricca, Vittorio Sambri
Sexually transmitted infections (STIs) have seen a considerable increase in the last years and given the health burden they may represent from both a personal and community perspective, they require surveillance and prevention programmes based on a timely and decentralized diagnosis. In this context, user-friendly rapid molecular tests may represent a good trade-off between diagnostic accuracy, accessibility and affordability. In this study we evaluated the diagnostic performance of a new real-time LAMP (Loop Mediated Isothermal Amplification) method for the rapid detection and differentiation of 7 major sexually transmissible pathogens by analysing real clinical samples (genital and extra-genital matrices) from individuals with suspected STIs...
2024: PloS One
https://read.qxmd.com/read/38511154/moving-towards-on-site-detection-of-shiga-toxin-producing-escherichia-coli-in-ready-to-eat-leafy-greens
#14
JOURNAL ARTICLE
Ana Costa-Ribeiro, Alexandre Lamas, Azucena Mora, Marta Prado, Alejandro Garrido-Maestu
Rapid identification of Shiga toxin-producing Escherichia coli , or STEC, is of utmost importance to assure the innocuousness of the foodstuffs. STEC have been implicated in outbreaks associated with different types of foods however, among them, ready-to-eat (RTE) vegetables are particularly problematic as they are consumed raw, and are rich in compounds that inhibit DNA-based detection methods such as qPCR. In the present study a novel method based on Loop-mediated isothermal amplification (LAMP) to overcome the limitations associated with current molecular methods for the detection of STEC in RTE vegetables targeting stx 1 and stx 2 genes...
2024: Current research in food science
https://read.qxmd.com/read/38510857/clinical-performance-of-the-reverse-transcription-loop-mediated-isothermal-amplification-assay-for-the-diagnosis-of-covid-19-in-a-thai-community-hospital-at-the-thailand-myanmar-border
#15
JOURNAL ARTICLE
Thanee Wongchai, Nuttagarn Chuenchom, Wiphat Klayut, Benjawan Phetsuksiri, Payu Bhakdeenuan, Supranee Bunchoo, Sopa Srisungngam, Janisara Rudeeaneksin
INTRODUCTION: Coronavirus disease 2019 (COVID-19) continues to be a global health threat and is a public health issue in Thailand and other countries. The extensive cross-border between Thailand and Myanmar is considered to be at a potentially high risk for COVID-19 distribution in this region. In this instance, simple and cost-effective tests for rapid and early detection of COVID-19 would be useful for effective patient management and control of the disease. METHODS: This study was conducted at Mae Sot Hospital on the border of Thailand-Myanmar to evaluate the diagnostic performance of a simple colorimetric reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay developed recently for the rapid detection of SARS-CoV-2...
February 2024: Curēus
https://read.qxmd.com/read/38506607/simultaneous-dual-gene-test-of-methicillin-resistant-staphylococcus-aureus-using-an-argonaute-centered-portable-and-visual-biosensor
#16
JOURNAL ARTICLE
Jun Kou, Yaru Li, Zhiying Zhao, Jiali Qiao, Qiang Zhang, Xiao Han, Xinkuan Cheng, Shuli Man, Long Ma
The development of novel method for drug-resistant bacteria detection is imperative. A simultaneous dual-gene Test of methicillin-resistant Staphylococcus aureus (MRSA) is developed using an Argonaute-centered portable biosensor (STAR). This is the first report concerning Argonaute-based pathogenic bacteria detection. Simply, the species-specific mecA and nuc gene are isothermally amplified using loop-mediated isothermal amplification (LAMP) technique, followed by Argonaute-based detection enabled by its programmable, guided, sequence-specific recognition and cleavage...
March 20, 2024: Small
https://read.qxmd.com/read/38494899/recent-advancements-with-loop-mediated-isothermal-amplification-lamp-in-assessment-of-the-species-authenticity-with-meat-and-seafood-products
#17
REVIEW
Shihui Wang, Hongwei Song, Tianlong Wang, Hanyue Xue, Yanjin Fei, Xiong Xiong
Species adulteration or mislabeling with meat and seafood products could negatively affect the fair trade, wildlife conservation, food safety, religion aspect, and even the public health. While PCR-based methods remain the gold standard for assessment of the species authenticity, there is an urgent need for alternative testing platforms that are rapid, accurate, simple, and portable. Owing to its ease of use, low cost, and rapidity, LAMP is becoming increasingly used method in food analysis for detecting species adulteration or mislabeling...
March 18, 2024: Critical Reviews in Food Science and Nutrition
https://read.qxmd.com/read/38481662/portable-and-ultrasensitive-hr-hpv-tests-based-on-nucleic-acid-biosensors
#18
REVIEW
Chang Ma, Minhong Zou, Ning Xu, Yan Liu, Yuru Wang
Cervical cancer is the third most common cancer threatening women's health globally, and high-risk human papillomavirus (HR-HPV) infection is the main cause of cervical cancer worldwide. Given the recurrent nature of HR-HPV infection, accurate screening is essential for its control. Since the commonly used polymerase chain reaction (PCR) technique is limited by professional equipment and personnel, convenient and ultrasensitive detection methods for HR-HPV are still highly needed. As new molecular detection methods, nucleic acid amplification-based biosensors have the advantages of high sensitivity, rapid operation, and portability, which are helpful for point-of-care testing in rural and remote areas...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38475862/diversity-of-culicoides-in-the-middle-belt-of-ghana-with-implications-on-the-transmission-of-mansonella-perstans-a-molecular-approach
#19
JOURNAL ARTICLE
Linda Batsa Debrah, Joseph F Arthur, Augustine Yeboah, Dorcas O Owusu, Ernest Adankwah, Isaac Acheampong, Difery Minadzi, Millicent Lamptey, Vera Serwaa Opoku, Wilfred Aniagyei, Monika M Vivekanandan, Mohammed K Abass, Amidu Gawusu, Samuel Wanji, Alexander Y Debrah, Marc Jacobsen, Richard O Phillips
BACKGROUND: Culicoides, also known as biting midges, carry pathogens which include Mansonella perstans. Mansonella perstans is a nematode parasite implicated in a number of disease outcomes. Even though a high prevalence of about 75% M. perstans infection has been recorded in some communities in the middle belt of Ghana, and a wide diversity of Culicoides species has been identified, the exact Culicoides species transmitting M. perstans in Ghana has not yet been deciphered. This study therefore aimed at assessing the species diversity of Culicoides and their role in the transmission of M...
March 12, 2024: Parasites & Vectors
https://read.qxmd.com/read/38467647/evaluation-of-molecular-inhibitors-of-loop-mediated-isothermal-amplification-lamp
#20
JOURNAL ARTICLE
May Khat Nwe, Nisachon Jangpromma, Lapatrada Taemaitree
Loop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform assay that enables the direct detection of DNA. Its use in point-of-care diagnostic tests is growing, while it has the potential to be used in presumptive on-the-field forensic tests. Samples are often collected from complex matrices that contain high levels of contaminants. Herein, we evaluate the effect of seven common DNA amplification inhibitors on LAMP - bile salts, calcium chloride, hematin, humic acid, immunoglobulin G, tannic acid and urea...
March 11, 2024: Scientific Reports
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