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https://www.readbyqxmd.com/read/28734968/identification-of-an-mitf-gene-and-its-polymorphisms-associated-with-the-vibrio-resistance-trait-in-the-clam-meretrix-petechialis
#1
Shujing Zhang, Xin Yue, Fengjuan Jiang, Hongxia Wang, Baozhong Liu
Microphthalmia-associated transcription factor (MITF) regulates the transcription of its target genes by binding to their promoters. In this study, an MITF gene, MpMITF was identified in the clam Meretrix petechialis. The full-length cDNA of MpMITF is 3564 bp with an ORF of 1365 bp. The deduced amino acid sequence consists of a conserved functional structure of bHLH-LZ, which could bind with E-box. The mRNA and protein expression levels of MpMITF were significantly up-regulated 6 h post-Vibrio injection. The mRNA expression of MpMITF increased on day 2 and peaked on day 10 post-Vibrio immersion...
July 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28733150/seven-new-lignan-glycosides-from-the-branches-of-alangium-kurzii-craib-var-laxifolium
#2
Jie Ren, Yang-Guo Xie, Ying-Ying Huang, Sheng-Lan Zhu, Shi-Kai Yan, Hui-Zi Jin, Wei-Dong Zhang
Seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of Alangium kurzii Craib var. laxifolium. Their structures were established by spectroscopic analysis and circular dichroism (CD) and X-ray analysis. The isolated compounds were evaluated for their antibacterial activities against Staphylococcus aureus CI1011, Streptococcus suis CI1608, Salmonella gallinarum CI0912, Enterococcus faecalis CI1304, Aeromonas hydrophila CI1008, Escherichia coli CI151012, Vibrio parahaemolyticus CI150506, Klebsiella pneumoniae CI131216, Pseudomonas aeruginosa CI1011, Staphylococcus epidermidis CI1110, and Streptococcus agalactiae CI1302...
July 18, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28732404/influence-of-the-stabilizers-on-the-toxicity-of-metallic-nanomaterials-in-aquatic-organisms-and-human-cell-lines
#3
Tatiana Andreani, Verónica Nogueira, Vera V Pinto, Maria José Ferreira, Maria Graça Rasteiro, Amélia M Silva, Ruth Pereira, Carlos M Pereira
In this study, following a systematic approach, we used aquatic species (bacteria Vibrio fischeri and microalgae Raphidocelis subcapitata) and different human cell lines (Caco-2, HepG2, SV-80 and HaCaT) representing different tissues and exposure pathways, to investigate how two organic stabilizers (PVA and DMSO) used for NMs dispersion influence their physicochemical properties, the persistence of metals in suspension and the toxicity/ecotoxicity of two metallic NMs (nano-Ag and nano-Cu). Although the stabilizers are expected to contribute to improve the dispersion and stability of NMs, the results obtained clearly showed that no similar changes in toxicity and morphological properties of the nano-Ag can be expected after its stabilization with PVA...
July 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28731682/cold-adaptation-of-triosephosphate-isomerase
#4
Johan Åqvist
The main problem for enzymes from psychrophilic species, that need to work near the freezing point of liquid water, is the exponential decay of reaction rates as the temperature is lowered. Cold-adapted enzymes have solved this problem by shifting the activation enthalpy-entropy balance for the catalyzed reaction compared to their mesophilic orthologs. To understand the structural basis of this universal feature it is necessary to examine pairs of such orthologous enzymes, with known 3D structures, at the microscopic level...
July 21, 2017: Biochemistry
https://www.readbyqxmd.com/read/28731211/comparative-pathogenicity-of-vibrio-spp-photobacterium-damselae-ssp-damselae-and-five-isolates-of-aeromonas-salmonicida-ssp-achromogenes-in-juvenile-atlantic-halibut-hippoglossus-hippoglossus
#5
T J Bowden, I R Bricknell, B M Preziosi
Juvenile Atlantic halibut (~100 mg, Hippoglossus hippoglossus) were exposed to Vibrio proteolyticus, a Vibrio spp. isolate, Photobacterium damselae ssp. damselae and five different isolates of Aeromonas salmonicida ssp. achromogenes via an hour-long bath immersion to ascertain their variation in pathogenicity to this fish species. Results were analysed using Kaplan-Meier survival analysis. Analysis of the data from challenges using A. salmonicida ssp. achromogenes revealed three survival values of zero and a spread of values from 0 to 28...
July 21, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28728852/in%C3%A2-vitro-characteristics-of-an-atlantic-salmon-salmo-salar-l-hind-gut-microbial-community-in-relation-to-different-dietary-treatments
#6
Kamarul Zaman Zarkasi, Richard S Taylor, Brett D Glencross, Guy C J Abell, Mark L Tamplin, John P Bowman
In this study, microbial community dynamics were assessed within a simple in vitro model system in order to understand those changes influenced by diet. The abundance and diversity of bacteria were monitored within different treatment slurries inoculated with salmon faecal samples in order to mimic the effects of dietary variables. A total of five complete diets and two ingredients (plant meal) were tested. The total viable counts (TVCs) and sequencing data revealed that there was very clear separation between the complete diets and the plant meal treatments, suggesting a dynamic response by the allochthonous bacteria to the treatments...
July 17, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28725407/the-contribution-of-marine-aggregate-associated-bacteria-to-the-accumulation-of-pathogenic-bacteria-in-oysters-an-agent-based-model
#7
Andrew M Kramer, J Evan Ward, Fred C Dobbs, Melissa L Pierce, John M Drake
Bivalves process large volumes of water, leading to their accumulation of bacteria, including potential human pathogens (e.g., vibrios). These bacteria are captured at low efficiencies when freely suspended in the water column, but they also attach to marine aggregates, which are captured with near 100% efficiency. For this reason, and because they are often enriched with heterotrophic bacteria, marine aggregates have been hypothesized to function as important transporters of bacteria into bivalves. The relative contribution of aggregates and unattached bacteria to the accumulation of these cells, however, is unknown...
October 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/28724932/simultaneous-identification-of-ten-bacterial-pathogens-using-the-multiplex-ligation-reaction-based-on-the-probe-melting-curve-analysis
#8
Yixiang Jiang, Lianhua He, Pingfang Wu, Xiaolu Shi, Min Jiang, Yinghui Li, Yiman Lin, Yaqun Qiu, Fang Bai, Yiqun Liao, Qingge Li, RenLi Zhang, Qinghua Hu
Pathogenic Vibrio spp., Aeromonas spp. and Plesiomonas shigelloides are associated with human gastroenteritis and wound infections, as well as fish diseases. The comprehensive and accurate identification of these pathogens is crucial for the current public health. The present study describes the development of a multiplex assay for the simultaneous identification of ten bacterial pathogens in a single reaction by using a multiplex ligation reaction based on probe melting curve analysis (MLMA). The specificity for target genes was 100%, as assessed with a panel of 67 bacterial pathogens, which indicated no cross-reactions...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724850/spatiotemporal-dynamics-of-total-viable-vibrio-spp-in-a-nw-mediterranean-coastal-area
#9
Léa Girard, Sébastien Peuchet, Pierre Servais, Annabelle Henry, Nadine Charni-Ben-Tabassi, Julia Baudart
A cellular approach combining Direct Viable Counting and Fluorescent In Situ Hybridization using a one-step multiple-probe technique and Solid Phase Cytometry (DVC-FISH-SPC) was developed to monitor total viable vibrios and cover the detection of a large diversity of vibrios. FISH combined three probes in the same assay and targeted sequences located at different positions on the 16S rRNA of Vibrio and Aliivibrio members. We performed a 10-month in situ study to investigate the weekly dynamics of viable vibrios relative to culturable counts at two northwestern Mediterranean coastal sites, and identified the key physicochemical factors for their occurrence in water using a multivariate analysis...
July 19, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28722617/case-report-disseminated-shewanella-algae-infection-with-meningoencephalitis-in-a-traveler-secondary-to-marine-injury-in-madagascar
#10
Caroline Brulliard, Nicolas Traversier, Jérôme Allyn, Christopher Schaeffer, Bruno Bouchet, Nicolas Allou
Marine microorganisms such as Shewanella spp., Vibrio spp., and Aeromonas spp. can cause sepsis secondary to a wound infection in the context of swimming. These microorganisms are most often susceptible to fluoroquinolones. Here, we report a unique case of Shewanella algae bacteremia associated with meningoencephalitis and disseminated via hematogenous spread secondary to a skin injury. The patient suffered the injury while swimming in saline water during a cruise holiday in Madagascar, and she was initially treated with amoxicillin...
June 12, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28722575/household-and-individual-risk-factors-for-cholera-among-cholera-vaccine-recipients-in-rural-haiti
#11
Wilfredo R Matias, Jessica E Teng, Isabelle J Hilaire, Jason B Harris, Molly F Franke, Louise C Ivers
Oral cholera vaccination was used as part of cholera control in Haiti, but the vaccine does not provide complete protection. We conducted secondary data analyses of a vaccine effectiveness study in Haiti to evaluate risk factors for cholera among cholera vaccine recipients. Individuals vaccinated against cholera that presented with acute watery diarrhea and had a stool sample positive for Vibrio cholerae O1 were included as cases. Up to four vaccinated individuals who did not present for treatment of diarrhea were included as controls for each case, and matched by location of residence, enrollment time, and age...
May 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28720083/a-prolonged-community-wide-cholera-outbreak-associated-with-drinking-water-contaminated-by-sewage-in-kasese-district-western-uganda
#12
Benon Kwesiga, Gerald Pande, Alex Riolexus Ario, Nazarius Mbona Tumwesigye, Joseph K B Matovu, Bao-Ping Zhu
BACKGROUND: In May 2015, a cholera outbreak that had lasted 3 months and infected over 100 people was reported in Kasese District, Uganda, where multiple cholera outbreaks had occurred previously. We conducted an investigation to identify the mode of transmission to guide control measures. METHODS: We defined a suspected case as onset of acute watery diarrhoea from 1 February 2015 onwards in a Kasese resident. A confirmed case was a suspected case with Vibrio cholerae O1 El Tor, serotype Inaba cultured from a stool sample...
July 18, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28719846/preparation-characterization-and-application-of-tio2-patterned-polyimide-film-as-a-photocatalyst-for-oxidation-of-organic-contaminants
#13
Subramaniyan Ramasundaram, Mingizem Gashaw Seid, Wonseop Lee, Chan Ul Kim, Eun-Ju Kim, Seok Won Hong, Kyoung Jin Choi
Photocatalytically active TiO2-patterned polyimide (PI) films (PI-TiO2) were fabricated using thermal transfer patterning (TTP). When subjected to hot pressing, the TiO2 nanoparticles electrosprayed on steel mesh templates were successfully transferred and formed checker plate patterns on PI film. FE-SEM studies confirmed that pressing at 350°C and 100MPa was optimum for obtaining patterns with uniform TiO2 coverage. When the quantity of TiO2 on the template increased, the amount of it immobilized on PI film also increased from 0...
June 29, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28719654/vibrio-vulnificus-induces-mtor-activation-and-inflammatory-responses-in-macrophages
#14
Dan-Li Xie, Meng-Meng Zheng, Yi Zheng, Hui Gao, Jie Zhang, Ting Zhang, Jian-Chun Guo, X Frank Yang, Xiao-Ping Zhong, Yong-Liang Lou
Vibrio vulnificus (V. vulnificus), a Gram-negative marine bacterium, can cause life-threatening primary septicemia, especially in patients with liver diseases. How V. vulnificus affects the liver and how it acts on macrophages are not well understood. In this report, we demonstrated that V. vulnificus infection causes a strong inflammatory response, marked expansion of liver-resident macrophages, and liver damage in mice. We demonstrated further that V. vulnificus activates mTOR in macrophages and inhibition of mTOR differentially regulates V...
2017: PloS One
https://www.readbyqxmd.com/read/28719320/skin-manifestations-of-primary-vibrio-vulnificus-septicemia
#15
Norman L Beatty, Jose Marquez, Mayar Al Mohajer
No abstract text is available yet for this article.
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28719281/risk-factors-for-household-transmission-of-vibrio-cholerae-in-dhaka-bangladesh-chobi7-trial
#16
Vanessa Burrowes, Jamie Perin, Shirajum Monira, David A Sack, Mahamud-Ur Rashid, Toslim Mahamud, Zillur Rahman, Munshi Mustafiz, Sazzadul I Bhuyian, Farzana Begum, Fatema Zohura, Shwapon Biswas, Tahmina Parvin, Tasdik Hasan, Xiaotong Zhang, Bradley R Sack, K M Saif-Ur-Rahman, Munirul Alam, Christine Marie George
AbstractHousehold contacts of cholera patients are at a 100 times higher risk of a Vibrio cholerae infection than the general population. To examine risk factors for V. cholerae infections and investigate intervention strategies among this population, we followed household contacts of cholera patients for the 1-week high-risk period after the index patient obtained care. This study was nested within a randomized controlled trial of the Cholera-Hospital-Based-Intervention-for-7-days (CHoBI7), a handwashing with soap and water treatment intervention in Dhaka, Bangladesh...
June 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28717834/characterizing-the-adherence-profiles-of-virulent-vibrio-parahaemolyticus-isolates
#17
Alisha M Aagesen, Sureerat Phuvasate, Yi-Cheng Su, Claudia C Häse
The human pathogen Vibrio parahaemolyticus is a leading cause of seafood-borne illness in the USA, and infections with V. parahaemolyticus typically result from eating raw or undercooked oysters. V. parahaemolyticus has been shown to be highly resistant to oyster depuration, suggesting that the bacterium possesses specific mechanisms or factors for colonizing oysters and persisting during depuration. In this study, we characterized eight different V. parahaemolyticus strains for differences in resistance to oyster depuration, biofilm formation, and motility...
July 17, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28716734/crystal-structure-analysis-of-3-6-anhydro-l-galactonate-cycloisomerase-suggests-emergence-of-novel-substrate-specificity-in-the-enolase-superfamily
#18
Saeyoung Lee, Kyoung Heon Kim, Hye-Yeon Kim, In-Geol Choi
3,6-Anydro-l-galatonate cycloisomerase (ACI) catalyzes the cycloisomerization of a 3,6-anhydro-l-galactonic acid known as a novel metabolite in agarolytic bacteria. Here, we present 3-D structures of ACI from Vibrio sp. strain EJY3 (VejACI) in native and mutant forms at 2.2 Å and 2.6 Å resolutions, respectively. The enzyme belongs to the mandelate racemase subgroup of the enolase superfamily catalyzing common β-elimination reactions by α-carbon deprotonation of substrates. The structure of VejACI revealed a notable 20s loop region in the capping domain, which can be a highly conserved structural motif in ACI homologs of agar metabolism...
July 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28716138/prevalence-of-enteric-infections-among-hospitalized-patients-in-two-referral-hospitals-in-ghana
#19
R Akuffo, G Armah, M Clemens, K C Kronmann, A H Jones, P Agbenohevi, K Sagoe, N Puplampu, N Talla Nzussouo, W Ampofo, K Koram, C Duplessis, E Dueger
BACKGROUND: Diarrhea is an important cause of morbidity and mortality worldwide. In Africa and Ghana in particular, it is estimated to contribute directly to 19 and 25% of pediatric mortality among children under 5 years, respectively. METHODS: Surveillance for hospitalized acute diarrheal illness was initiated in November 2010 through October 2012 in a referral hospital in southern Ghana, and a teaching hospital in northern Ghana. Consenting hospitalized patients who met a standardized case definition for acute diarrheal illness provided demographic and epidemiologic data...
July 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28714216/contributions-of-the-oligopeptide-permeases-in-multistep-of-vibrio-alginolyticus-pathogenesis
#20
Wenjia Liu, Lixing Huang, Yongquan Su, Yingxue Qin, Lingmin Zhao, Qingpi Yan
Vibrio alginolyticus has been associated with several diseases of cultivated marine animals, and has led to considerable economic losses. The oligopeptide permease (Opp) has been proven to play a variety of important roles in nutrition and virulence in several bacteria. In our previous research, the opp gene cluster was identified in Vibrio alginolyticus with transcriptome sequence, which also indicated that the Opp system might play roles in the regulation of adhesion. In this study, the relationship between V...
July 17, 2017: MicrobiologyOpen
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