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https://www.readbyqxmd.com/read/28527323/revealing-the-ability-of-a-novel-polysaccharide-bioflocculant-in-bioremediation-of-heavy-metals-sensed-in-a-vibrio-bioluminescence-reporter-assay
#1
Arya Sajayan, G Seghal Kiran, S Priyadharshini, Navya Poulose, Joseph Selvin
A bioflocculant-producing bacterial strain, designated MSI021, was isolated from the marine sponge Dendrilla nigra and demonstrated 94% flocculation activity in a kaolin clay suspension. MSI021 was identified as Bacillus cereus based on phylogenetic affiliation and biochemical characteristics. The purified extra-cellular bioflocculant was chemically elucidated as a polysaccharide molecule. The polysaccharide bioflocculant was stable under both acidic and alkaline conditions (pH 2.0-10.0) and temperatures up to 100 °C...
May 17, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28525966/gene-expression-of-vibrio-parahaemolyticus-growing-in-laboratory-isolation-conditions-compared-to-those-common-in-its-natural-ocean-environment
#2
Katherine García, Cristian Yáñez, Nicolás Plaza, Francisca Peña, Pedro Sepúlveda, Diliana Pérez-Reytor, Romilio T Espejo
BACKGROUND: Vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °C, a condition seldom found in the ocean. However, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. In this context, revealing how gene expression enables V. parahaemolyticus to adapt to this particular condition -common to almost all V. parahaemolyticus isolates- will improve our understanding of the biology of this important pathogen...
May 19, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28523368/two-stage-prediction-on-effects-of-mixtures-containing-phenolic-compounds-and-heavy-metals-on-vibrio-qinghaiensis-sp-q67
#3
Ling-Yun Mo, Jie Liu, Li-Tang Qin, Hong-Hu Zeng, Yan-Peng Liang
Two-stage prediction (TSP) model had been developed to predict toxicities of mixtures containing complex components, but its prediction power need to be further validated. Six phenolic compounds and six heavy metals were selected as mixture components. One mixture (M1) was built with equivalent-effect concentration ratio and four mixtures (M2-M5) were designed with fixed concentration ratio. In M1-M5, the toxicities were well predicted by TSP model, while CA overestimated and IA underestimated the toxicities...
May 18, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28522707/draft-genome-sequence-of-the-luminescent-strain-vibrio-campbellii-lb102-isolated-from-a-black-tiger-shrimp-penaeus-monodon-broodstock-rearing-system
#4
Sujeet Kumar, Ashok Kumar Jangam, V Akhil, Vidya Rajendran, Vinaya Kumar Katneni, J Joseph Sahaya Rajan, Monendra Grover, Viswas Konasagara Nagaleekar, S V Alavandi, K K Vijayan
We report here the genome sequence of Vibrio campbellii LB102, isolated from the broodstock rearing system of a shrimp hatchery in India. Sequence analysis revealed the presence of effector toxins of the type III (YopT, sharing 39% identity with Yersinia pestis) and type VI (VgrG-3 and hemolysin coregulated protein of V. cholerae) secretion systems.
May 18, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28522420/in-vitro-effects-of-italian-lavandula-multifida-l-leaf-extracts-on-gilthead-seabream-sparus-aurata-leucocytes-and-saf-1-cells
#5
Angela Fazio, Rebeca Cerezuela, Maria Rosaria Panuccio, Alberto Cuesta, Maria Ángeles Esteban
Lavandula multifida is very appreciated by pharmaceutical and cosmetic industries. In Italy is only found in Calabria and Sicily and, at present, urge its valorization due to its high extinction and genetic erosion risks. Possible applications of L. multifida extracts as immunostimulant in fish aquaculture were assayed by using gilthead seabream (Sparus aurata) as a marine fish model, due to its importance in fish aquaculture. The in vitro effects of both aqueous and ethanolic leaf extracts obtained from two Italian populations of L...
May 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28522419/effects-of-stocking-density-of-the-white-shrimp-litopenaeus-vannamei-boone-on-immunities-antioxidant-status-and-resistance-against-vibrio-harveyi-in-a-biofloc-system
#6
Gang Liu, Songming Zhu, Dezhao Liu, Xishan Guo, Zhangying Ye
Determining optimum stocking density of the white shrimp Litopenaeus vannamei (Boone) is a big concern for shrimp farmers. However, few studies have assessed the influence of stocking density on the antioxidant status, immunology, digestive enzyme activities, and growth performance of white shrimp in biofloc systems. In this study, these parameters of white shrimp in a biofloc system were compared at three stocking densities: 300 orgs m(-3) as low stocking density (LD), 400 orgs m(-3) as medium stocking density (MD), and 500 orgs m(-3) as high stocking density (HD)...
May 15, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28516064/a-novel-role-of-listeria-monocytogenes-membrane-vesicles-in-inhibition-of-autophagy-and-cell-death
#7
Svitlana Vdovikova, Morten Luhr, Paula Szalai, Lars Nygård Skalman, Monika K Francis, Richard Lundmark, Nikolai Engedal, Jörgen Johansson, Sun N Wai
Bacterial membrane vesicle (MV) production has been mainly studied in Gram-negative species. In this study, we show that Listeria monocytogenes, a Gram-positive pathogen that causes the food-borne illness listeriosis, produces MVs both in vitro and in vivo. We found that a major virulence factor, the pore-forming hemolysin listeriolysin O (LLO), is tightly associated with the MVs, where it resides in an oxidized, inactive state. Previous studies have shown that LLO may induce cell death and autophagy. To monitor possible effects of LLO and MVs on autophagy, we performed assays for LC3 lipidation and LDH sequestration as well as analysis by confocal microscopy of HEK293 cells expressing GFP-LC3...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28515714/new-insights-into-pathogenic-vibrios-affecting-bivalves-in-hatcheries-present-and-future-prospects
#8
REVIEW
Javier Dubert, Juan L Barja, Jesús L Romalde
Hatcheries constitute nowadays the only viable solution to support the husbandry of bivalve molluscs due to the depletion and/or overexploitation of their natural beds. Hatchery activities include the broodstock conditioning and spawning, rearing larvae and spat, and the production of microalgae to feed all stages of the production cycle. However, outbreaks of disease continue to be the main bottleneck for successful larval and spat production, most of them caused by different representatives of the genus Vibrio...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28514430/erratum-a-vibrio-cholerae-autoinducer-receptor-pair-that-controls-biofilm-formation
#9
Kai Papenfort, Justin E Silpe, Kelsey R Schramma, Jian-Ping Cong, Mohammad R Seyedsayamdost, Bonnie L Bassler
No abstract text is available yet for this article.
May 17, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28514180/molecular-typing-of-vibrio-parahaemolyticus-strains-isolated-from-mollusks-in-the-north-adriatic-sea
#10
Mohammad Shamsur Rahman, Roberta Carraro, Barbara Cardazzo, Lisa Carraro, Davide Boscolo Meneguolo, Maria Elena Martino, Nadia Andrea Andreani, Paola Bordin, Renzo Mioni, Lisa Barco, Enrico Novelli, Stefania Balzan, Luca Fasolato
BACKGROUND: Vibrio parahaemolyticus is an emerging foodborne pathogen in the Mediterranean, usually associated with shellfish consumption. The increase in the number of outbreaks in Europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. The primary role of Italy in Europe's mollusk production, together with the fact that cases of infections with V. parahaemolyticus are not always notified to the European community, highlighted the necessity of acquiring new information about the epidemiological involvement of shellfish products...
May 17, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28512146/science-signaling-podcast-for-16-may-2017-vibrio-rewires-host-cells
#11
Nicole J De Nisco, Kim Orth, Annalisa M VanHook
This Podcast features a conversation with Kim Orth and Nicole De Nisco, authors of a Research Resource that appears in the 16 May 2017 issue of Science Signaling, about how the marine bacterium Vibrio parahaemolyticus rewires host cell signaling networks. V. parahaemolyticus thrives in warm brackish waters and infects both shellfish and finfish. This bacterium causes gastroenteritis when humans consume contaminated seafood that is raw or undercooked. V. parahaemolyticus delivers virulence factors into host cells through two different type 3 secretion systems (T3SSes)...
May 16, 2017: Science Signaling
https://www.readbyqxmd.com/read/28512145/the-cytotoxic-type-3-secretion-system-1-of-vibrio-rewires-host-gene-expression-to-subvert-cell-death-and-activate-cell-survival-pathways
#12
Nicole J De Nisco, Mohammed Kanchwala, Peng Li, Jessie Fernandez, Chao Xing, Kim Orth
Bacterial effectors potently manipulate host signaling pathways. The marine bacterium Vibrio parahaemolyticus (V. para) delivers effectors into host cells through two type 3 secretion systems (T3SSs). T3SS1 is vital for V. para survival in the environment, whereas T3SS2 causes acute gastroenteritis in human hosts. Although the natural host is undefined, T3SS1 effectors attack highly conserved cellular processes and pathways to orchestrate nonapoptotic cell death. To understand how the concerted action of T3SS1 effectors globally affects host cell signaling, we compared gene expression changes over time in primary fibroblasts infected with V...
May 16, 2017: Science Signaling
https://www.readbyqxmd.com/read/28512090/the-vibrio-cholerae-vexgh-rnd-efflux-system-maintains-cellular-homeostasis-by-effluxing-vibriobactin
#13
Dillon E Kunkle, X Renee Bina, James E Bina
Resistance-nodulation-division (RND) superfamily efflux systems have been widely studied for their role in antibiotic resistance, but their native biological functions remain poorly understood. We previously showed that loss of RND-mediated efflux in Vibrio cholerae resulted in activation of the Cpx two-component regulatory system, which mediates adaptation to stress resulting from misfolded membrane proteins. Here, we investigated the mechanism linking RND-mediated efflux to the Cpx response. We performed transposon mutagenesis screening of RND-deficient V...
May 16, 2017: MBio
https://www.readbyqxmd.com/read/28511613/antimicrobial-activity-and-molecular-docking-studies-of-a-novel-anthraquinone-from-a-marine-derived-fungus-aspergillus-versicolor
#14
Weiyi Wang, Ruixuan Chen, Zhuhua Luo, Wei Wang, Jianming Chen
A novel anthraquinone, 2-(dimethoxymethyl)-1-hydroxyanthracene-9,10-dione (1), together with nine known compounds (2-10), were isolated from the fermentation of Aspergillus versicolor derived from deep sea sediment. Their structures were established through spectroscopic methods. Compound 1 exhibited strong inhibitory activities against MRSA ATCC 43300 and MRSA CGMCC 1.12409 (with MIC values of 3.9 and 7.8 μg/mL respectively) and moderate activities against tested strains of Vibrio (with MIC values ranging from 15...
May 16, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28507104/genetic-characterization-of-broad-host-range-incq-plasmids-harboring-blaveb-18-in-vibrio-spp
#15
Ruichao Li, Lianwei Ye, Zhiwei Zheng, Edward Wai Chi Chan, Sheng Chen
Dissemination of genetic elements encoding VEB type β-lactamases among Gram-negative bacteria has become a public health concern worldwide (1, 2).….
May 15, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28505584/development-of-a-gold-nanoparticle-based-universal-oligonucleotide-microarray-for-multiplex-and-low-cost-detection-of-foodborne-pathogens
#16
Xiaoqiang Wang, Sisi Ying, Xiaoguang Wei, Jun Yuan
Bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. In this study a novel assay, combining gold nanoparticle (GNP)-based multiplex oligonucleotide ligation-PCR and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including Shigella spp., Campylobacter jejuni, Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, Staphylococcus aureus, and Vibrio parahaemolyticus...
May 9, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28505561/class-enzyme-based-motors-for-on-the-fly-enantiomer-analysis-of-amino-acids
#17
Laura García-Carmona, María Moreno-Guzmán, María Cristina González, Alberto Escarpa
Here, two class-enzyme motors are properly designed allowing the rapid dispersion of the class-enzyme D-amino acid oxidase (DAO) and L-amino acid oxidase (LAO) for selective "on the fly" biodetection of D and L-amino acids (AAs), respectively. The efficient movement together with the continuous release of fresh class-enzyme leads to a greatly accelerated enzymatic reaction processes without the need of external stirring or chemical and physical attachment of the enzyme. Ultra-fast detection (<2min) and accurate quantifications of L-phenylalanine (L-Phe) in plasma and whole-blood newborns samples diagnosed with Phenylketonuria and total D-AAs in Vibrio cholera cultures are pioneer illustrated as relevant examples of each enantiomer determination...
May 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28504816/virulence-factors-and-antimicrobial-resistance-in-environmental-strains-of-vibrio-alginolyticus
#18
Marcos F Hernández-Robles, Ana K Álvarez-Contreras, Patricia Juárez-García, Iván Natividad-Bonifacio, Everardo Curiel-Quesada, Carlos Vázquez-Salinas, Elsa I Quiñones-Ramírez
Vibrio alginolyticus has acquired increasing importance because this microorganism may be pathogenic to aquatic animals and humans. It has been reported that some V. alginolyticus strains carry virulence genes derived from pathogenic V. cholerae and V. parahaemolyticus strains. In this work V. alginolyticus was isolated from oyster samples acquired from a food-market in Mexico City. Thirty isolates were identified as V. alginolitycus. Strains showed β-haemolysis and proteolytic activity and produced a capsule...
December 2016: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28504641/helical-jackknives-control-the-gates-of-the-double-pore-k-uptake-system-ktrab
#19
Marina Diskowski, Ahmad Reza Mehdipour, Dorith Wunnicke, Deryck J Mills, Vedrana Mikusevic, Natalie Bärland, Jan Hoffmann, Nina Morgner, Heinz-Jürgen Steinhoff, Gerhard Hummer, Janet Vonck, Inga Haenelt
Ion channel gating is essential for cellular homeostasis and is tightly controlled. In some eukaryotic and most bacterial ligand-gated K(+) channels, RCK domains regulate ion fluxes. Until now, a single regulatory mechanism has been proposed for all RCK-regulated channels, involving signal transduction from the RCK domain to the gating area. Here we present an inactive ADP-bound structure of KtrAB from Vibrio alginolyticus, determined by cryo-electron microscopy, which, combined with EPR spectroscopy and molecular dynamics simulations, uncovers a novel regulatory mechanism for ligand-induced action at a distance...
May 15, 2017: ELife
https://www.readbyqxmd.com/read/28501446/the-involvement-of-cathepsin-f-gene-ctsf-in-turbot-scophthalmus-maximus-l-mucosal-immunity
#20
Chengbin Gao, Qiang Fu, Baofeng Su, Huanhuan Song, Shun Zhou, Fenghua Tan, Chao Li
Cathepsin F (CTSF) is a recently described papain-like cysteine protease and unique among cathepsins due to an elongated N-terminal pro-region, which contains a cystatin domain. CTSF likely plays a regulatory role in processing the invariant chain which is associated with the major histocompatibility complex (MHC) class II. In this regard, we identified the CTSF gene of turbot as well as its protein structure, phylogenetic relationships, and expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge...
May 10, 2017: Fish & Shellfish Immunology
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