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https://www.readbyqxmd.com/read/29781755/more-robust-gut-immune-responses-induced-by-combining-intranasal-and-sublingual-routes-for-prime-boost-immunization
#1
Hye Suk Hwang, Sao Puth, Wenzhi Tan, Vivek Verma, Kwangjoon Jeong, Shee Eun Lee, Joon Haeng Rhee
Norovirus causes acute and debilitating gastroenteritis, characterized by vomiting and diarrhea. We recently reported a recombinant GII. 4 P domain particle (Pd) vaccine adjuvanted with a flagellin, Vibrio vulnificus FlaB, effectively promoting both humoral and cell-mediated immune responses. In the previous study, we found that sublingual (SL) immunization induced higher fecal secretory IgA (SIgA) responses while intranasal (IN) route provided higher amplitude of humoral and cellular immune responses in the systemic compartment...
May 21, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29778843/a-synbiotic-improves-the-immunity-of-white-shrimp-litopenaeus-vannamei-metabolomic-analyses-reveal-compelling-evidence
#2
Truong-Giang Huynh, Ann-Chang Cheng, Chia-Chun Chi, Kuo-Hsun Chiu, Chun-Hung Liu
In this study, we examined the synergistic effects of a diet-administered synbiotic comprising galactooligosaccharide (GOS) and the probiotic Lactobacillus plantarum 7-40 on immune responses, immune-related gene expressions, and disease resistance to Vibrio alginolyticus in white shrimp Litopenaeus vannamei. To unravel the regulatory role of the synbiotic in activating the immune system of shrimp, 1 H nuclear magnetic resonance (NMR)-based metabolomic analyses were used to investigate hepatopancreas metabolites, then significantly altered metabolites were confirmed in both the hepatopancreas and plasma by reverse-phase high-performance liquid chromatography (RP-HPLC) and spectrophotometric analyses...
May 17, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29777309/fluorometric-determination-of-vibrio-parahaemolyticus-using-an-f-0-f-1-atpase-based-aptamer-and-labeled-chromatophores
#3
Nuo Duan, Shijia Wu, Huiling Zhang, Ying Zou, Zhouping Wang
An F0 F1 -ATPase-based aptasensor is described for the fluorometric determination of Vibrio parahaemolyticus. Chromatophores containing F0 F1 -ATPases were first prepared from Rhodospirillum rubrum cells. Then, an aptamer-functionalized chromatophore acts as the capture probe, and a chromatophore labeled with the pH probe fluorescein acts as the signalling probe. In the presence of V. parahaemolyticus, the rotation rate of F0 F1 -ATPase is decreased due to the formation of the aptamer-chromatophore complex...
May 18, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29776924/tools-for-rapid-genetic-engineering-of-vibrio-fischeri
#4
Karen L Visick, Kelsey M Hodge-Hanson, Alice H Tischler, Allison K Bennett, Vincent Mastrodomenico
Vibrio fischeri is used as a model for number of processes, including symbiosis, quorum sensing, bioluminescence, and biofilm formation. Many of these studies depend on generating deletion mutants and complementing them. Engineering such strains, however, is a time-consuming, multi-step process that relies on cloning and subcloning. Here, we describe a set of tools that can be used to rapidly engineer deletions and insertions in the V. fischeri chromosome without cloning. We developed a uniform approach for generating deletions using PCR SOEing (Splicing by Overlap Extension) with antibiotic cassettes flanked by standardized linker sequences...
May 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29775727/isolation-structure-elucidation-and-antibacterial-activity-of-methyl-4-8-dimethylundecanate-from-the-marine-actinobacterium-streptomyces-albogriseolus-ecr64
#5
Durairaj Thirumurugan, Ramasamy Vijayakumar, Chithravel Vadivalagan, Pushparaj Karthika, Md Khurshid Alam Khan
Around 120 actinobacterial colonies were isolated from various regions of marine East coast region of Tamil Nadu, India. Among them, 33 were morphologically distinct and they were preliminarily screened for their antibacterial activity against Pseudomonas fluorescens, Vibrio cholerae, V. parahaemolyticus, V. alginolyticus, and Aeromonas hydrophila by cross-streak plate technique. Among the isolated, the isolate ECR64 exhibited maximum zone of inhibition against fish pathogenic bacteria. The crude bioactive compounds were extracted from the isolate ECR64 using different organic solvents which exhibited maximum antibacterial activity...
May 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29773633/complete-genome-sequences-of-two-bioluminescent-vibrio-campbellii-strains-isolated-from-biofouling-communities-in-the-bay-of-bengal
#6
Sophie M Colston, Gregory A Ellis, Seongwon Kim, Hemantha W Wijesekera, Dagmar H Leary, Baochuan Lin, Benjamin C Kirkup, W Judson Hervey, Gary J Vora
Vibrio campbellii is a pathogen of aquatic animals and has been proposed as a bacterial partner in the formation of bioluminescent milky seas. We present here the complete genome sequences assembled from Illumina and Oxford Nanopore data for two bioluminescent Vibrio campbellii strains (BoB-53 and BoB-90) isolated from biofouled moorings in the Bay of Bengal.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29773632/complete-genome-sequence-of-oyster-isolate-vibrio-vulnificus-env1
#7
Parisa Noorian, Shuyang Sun, Diane McDougald
Vibrio vulnificus , a ubiquitous inhabitant of coastal marine environments, has been isolated from a variety of sources. It is an opportunistic pathogen of both marine animals and humans. Here, the genome sequence of V. vulnificus Env1, an environmental isolate resistant to predation by the ciliate Tetrahymena pyriformis , is reported.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29773630/draft-genome-sequence-of-a-new-vibrio-strain-with-the-potential-to-produce-bacteriocin-like-inhibitory-substances-isolated-from-the-gut-microflora-of-scallop-argopecten-purpuratus
#8
Wilbert Serrano, Ulrike I Tarazona, Raul M Olaechea, Michael W Friedrich
A new Vibrio strain, V7A, was isolated from the intestinal tract of the Peruvian scallop ( Argopecten purpuratus ). Strain V7A clusters within the Mediterranei clade of the genus Vibrio and has the potential to produce bacteriocin-like inhibitory substances (BLIS). Here, we report the draft genome sequence of Vibrio mediterranei strain V7A.
May 17, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29772736/exploring-the-effect-of-phage-therapy-in-preventing-vibrio-anguillarum-infections-in-cod-and-turbot-larvae
#9
Nanna Rørbo, Anita Rønneseth, Panos G Kalatzis, Bastian Barker Rasmussen, Kirsten Engell-Sørensen, Hans Petter Kleppen, Heidrun Inger Wergeland, Lone Gram, Mathias Middelboe
The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to antibiotics and inefficient vaccination at the larval stage of fish emphasizes the need for novel approaches, and phage therapy for controlling Vibrio pathogens has gained interest in the past few years. In this study, we examined the potential of the broad-host-range phage KVP40 to control four different V...
May 16, 2018: Antibiotics
https://www.readbyqxmd.com/read/29772373/a-novel-c-type-lectin-from-the-sea-cucumber-apostichopus-japonicus-ajctl-2-with-preferential-binding-of-d-galactose
#10
Hui Wang, Zhuang Xue, Zhaoqun Liu, Weilin Wang, Feifei Wang, Ying Wang, Lingling Wang, Linsheng Song
C-type lectins (CTLs) are Ca2+ dependent carbohydrate-binding proteins that share structural homology in their carbohydrate-recognition domains (CRDs). In the present study, a novel CTL was identified from sea cucumber Apostichopus japonicus (named as AjCTL-2). The deduced amino acid sequence of AjCTL-2 was homologous to CTLs from other animals with the identities ranging from 33% to 40%. It contained a canonical signal peptide at the N-terminus, a low density lipoprotein receptor class A (LDLa), a C1r/C1s/Uegf/bone morphogenetic protein 1 (CUB), and a CRD with two motif Glu-Pro-Asn (EPN) and Trp-Asn-Asp (WND) in Ca2+ binding site 2...
May 14, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29771877/cholera-epidemic-lusaka-zambia-october-2017-may-2018
#11
Nyambe Sinyange, Joan M Brunkard, Nathan Kapata, Mazyanga Lucy Mazaba, Kunda G Musonda, Raymond Hamoonga, Muzala Kapina, Fred Kapaya, Lwito Mutale, Ernest Kateule, Francis Nanzaluka, James Zulu, Chileshe Lukwesa Musyani, Alison V Winstead, William W Davis, Hammad S N'cho, Nelia L Mulambya, Patrick Sakubita, Orbie Chewe, Sulani Nyimbili, Ezinne V C Onwuekwe, Nedghie Adrien, Anna J Blackstock, Travis W Brown, Gordana Derado, Nancy Garrett, Sunkyung Kim, Sydney Hubbard, Amy M Kahler, Warren Malambo, Eric Mintz, Jennifer Murphy, Rupa Narra, Gouthami G Rao, Margaret A Riggs, Nicole Weber, Ellen Yard, Khozya D Zyambo, Nathan Bakyaita, Namani Monze, Kennedy Malama, Jabbin Mulwanda, Victor M Mukonka
On October 6, 2017, an outbreak of cholera was declared in Zambia after laboratory confirmation of Vibrio cholerae O1, biotype El Tor, serotype Ogawa, from stool specimens from two patients with acute watery diarrhea. The two patients had gone to a clinic in Lusaka, the capital city, on October 4. Cholera cases increased rapidly, from several hundred cases in early December 2017 to approximately 2,000 by early January 2018 (Figure). In collaboration with partners, the Zambia Ministry of Health (MoH) launched a multifaceted public health response that included increased chlorination of the Lusaka municipal water supply, provision of emergency water supplies, water quality monitoring and testing, enhanced surveillance, epidemiologic investigations, a cholera vaccination campaign, aggressive case management and health care worker training, and laboratory testing of clinical samples...
May 18, 2018: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/29771875/notes-from-the-field-outbreak-of-vibrio-cholerae-associated-with-attending-a-funeral-chegutu-district-zimbabwe-2018
#12
Jarred B McAteer, Sydney Danda, Tonderai Nhende, Paul Manamike, Tonderai Parayiwa, Andrew Tarupihwa, Ottias Tapfumanei, Portia Manangazira, Gibson Mhlanga, Daniela B Garone, Andrea Martinsen, Rachael D Aubert, William Davis, Rupa Narra, Shirish Balachandra, Beth A Tippett Barr, Eric Mintz
No abstract text is available yet for this article.
May 18, 2018: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/29770134/comparative-immune-and-stress-related-transcript-response-induced-by-air-exposure-and-vibrio-anguillarum-bacterin-in-rainbow-trout-oncorhynchus-mykiss-and-gilthead-seabream-sparus-aurata-mucosal-surfaces
#13
Ali Reza Khansari, Joan Carles Balasch, Eva Vallejos-Vidal, David Parra, Felipe E Reyes-López, Lluís Tort
Fish have to face various environmental challenges that may compromise the efficacy of the immune response in mucosal surfaces. Since the effect of acute stress on mucosal barriers in fish has still not been fully elucidated, we aimed to compare the short-term mucosal stress and immune transcriptomic responses in a freshwater (rainbow trout, Oncorhynchus mykiss ) and a marine fish (gilthead seabream, Sparus aurata ) to bacterial immersion ( Vibrio anguillarum bacterin vaccine) and air exposure stress in skin, gills, and intestine...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29769498/quantitative-analysis-of-nucleic-acid-extraction-methods-for-vibrio-cholerae-using-real-time-pcr-and-conventional-pcr
#14
Z Z Hossain, J Ferdous, S M Tulsiani, P M Jensen, A Begum
The devastating diarrheal disease cholera is caused by the bacterium Vibrio cholerae. Nucleic acid extraction is the primary step for several molecular detection approaches. In order to identify the sources of cholera illness, an efficient, fast and easy DNA extraction method for toxigenic Vibrio cholerae is desired which can be applied on diverse type of samples. This methodology developmental setup study was performed in the Environmental Microbiology Lab, Department of Microbiology, University of Dhaka, Bangladesh from March 2015 to May 2015 attempted to compare three DNA extraction methods for efficient detection and quantification of Vibrio cholerae...
April 2018: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/29768238/one-pot-green-fabrication-of-metallic-silver-nanoscale-materials-using-crescentia-cujete-l-and-assessment-of-their-bactericidal-activity
#15
Seetharaman Prabukumar, Chandrasekaran Rajkuberan, Gnanasekar Sathishkumar, Mani Illaiyaraja, Sivaperumal Sivaramakrishnan
In this study, the leaf extract of an important medicinal plant Crescentia cujete L. (CC) was employed as a green reducing agent to synthesise highly-stable C. cujete silver nanoparticles (CCAgNPs). The reduction of Ag+ to Ag0 nanoparticles was initially observed by a colour change which generates an intense surface plasmon resonance peak at 417 nm using a UV-Vis spectrophotometer. Various optimisation factors such as temperature, pH, time and the stoichiometric proportion of the reaction mixture were performed, which influence the size, dispersity and synthesis rate of CCAgNPs...
June 2018: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29767342/characterization-of-xanthine-dehydrogenase-and-aldehyde-oxidase-of-marsupenaeus-japonicus-and-their-response-to-microbial-pathogen
#16
Yo Okamura, Mari Inada, Gehad Elsaid Elshopakey, Toshiaki Itami
Reactive oxygen species (ROS) play key roles in many physiological processes. In particular, the sterilization mechanism of bacteria using ROS in macrophages is a very important function for biological defense. Xanthine dehydrogenase (XDH) and aldehyde oxidase (AOX), members of the molybdo-flavoenzyme subfamily, are known to generate ROS. Although these enzymes occur in many vertebrates, some insects, and plants, little research has been conducted on XDHs and AOXs in crustaceans. Here, we cloned the entire cDNA sequences of XDH (MjXDH: 4328 bp) and AOX (MjAOX: 4425 bp) from Marsupenaeus japonicus (kuruma shrimp) using reverse transcriptase-polymerase chain reaction (RT-PCR) and random amplification of cDNA ends (RACE)...
May 16, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29765621/biofilm-inhibition-and-pathogenicity-attenuation-in-bacteria-by-proteus-mirabilis
#17
Shichen Yu, Xiaoshan Zhu, Jin Zhou, Zhonghua Cai
Biofilms play an important role in the antibiotic resistance of encased bacteria, and biofilm formation is regulated by quorum sensing (QS). Inhibiting the QS system may, therefore, degrade the integrity of a biofilm and expose the bacterial pathogens within it to the deleterious effects of molecules such as antibiotics. Moreover, the use of QS inhibitors (QSIs) may provide a novel approach for treating bacterial infections of aquacultures. In the present study, the bacterium Proteus mirabilis was identified as a potential producer of QSIs...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29765369/commentary-genome-sequence-of-vibrio-parahaemolyticus-vp152-strain-isolated-from-penaeus-indicus-in-malaysia
#18
COMMENT
Theodore Allnutt, Chrystine Zou Yi Yan, Tamsyn M Crowley, Han Ming Gan
No abstract text is available yet for this article.
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29763968/binding-to-type-i-collagen-is-essential-for-the-infectivity-of-vibrio-parahaemolyticus-to-host-cells
#19
Ming Liu, Shanshan Yang, Chengkun Zheng, Xuesong Luo, Weicheng Bei, Peng Cai
Vibrio parahaemolyticus is a globally present marine bacterium that often leads to acute gastroenteritis. Two type III secretion systems (T3SSs), T3SS1 and T3SS2, are important for host infection. Type I collagen is a component of the extracellular matrix and is abundant in the small intestine. However, whether type I collagen serves as the cellular receptor for V. parahaemolyticus infection of host cells remains enigmatic. In this study, we discovered that type I collagen is not only important for the attachment of V...
May 15, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29762521/structure-of-the-exopolysaccharide-secreted-by-a-marine-strain-vibrio-alginolyticus
#20
Sophie Drouillard, Isabelle Jeacomine, Laurine Buon, Claire Boisset, Anthony Courtois, Bertrand Thollas, Pierre-Yves Morvan, Romuald Vallée, William Helbert
Vibrio alginolyticus (CNCM I-4151) secretes an exopolysaccharide whose carbohydrate backbone is decorated with amino acids, likely conferring its properties that are appreciated in cosmetics. Here, the secreted polysaccharide of another strain of V. alginolyticus (CNCM I-5034) was characterized by chromatography and one- and two-dimensional NMR spectroscopy experiments. The structure was resolved and shows that the carbohydrate backbone is made of four residues: D-galactose (Gal), D-galacturonic acid (GalA) D-N-acetylglucosamine (GlcNAc) and D-glucuronic acid (GlcA), forming a tetrasaccharide repetition unit [→4)-β-d-GlcA-(1→3)-α-d-Gal-(1→3)-α-d-GalA-(1→3)-β-GlcNAc(1→]...
May 15, 2018: Marine Drugs
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