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https://www.readbyqxmd.com/read/28330075/a-novel-multiplex-pcr-method-for-the-detection-of-virulence-associated-genes-of-escherichia-coli-o157-h7-in-food
#1
Vo Van Giau, Thuy Trang Nguyen, Thi Kim Oanh Nguyen, Thi Thuy Hang Le, Tien Dung Nguyen
Shiga toxin-producing Escherichia coli O157:H7 (E. coli O157:H7) strains are foodborne infectious agents that cause a number of life-threatening diseases, including hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Shiga toxin 1 (stx1), shiga toxin 2 (stx2), or a combination of both are responsible for most clinical symptoms of these diseases. Hence, various diagnostic methods have been developed so far to detect shiga toxins such as cell culture, ELISA, Rapid Latex Agglutination (RPLA) and hybridization, but due to high costs and labor time in addition to low sensitivity, they have not received much attention...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329394/intermediate-follow-up-of-pediatric-patients-with-hemolytic-uremic-syndrome-during-the-2011-outbreak-caused-by-e-coli-o104-h4
#2
Sebastian Loos, Wiebke Aulbert, Bernd Hoppe, Thurid Ahlenstiel-Grunow, Birgitta Kranz, Charlotte Wahl, Hagen Staude, Alexander Humberg, Kerstin Benz, Martin Krause, Martin Pohl, Max C Liebau, Raphael Schild, Johanna Lemke, Ortraud Beringer, Dominik Müller, Christoph Härtel, Marianne Wigger, Udo Vester, Martin Konrad, Dieter Haffner, Lars Pape, Jun Oh, Markus J Kemper
Background.: In 2011 Escherichia coli O104:H4 caused an outbreak with over 800 cases of hemolytic uremic syndrome (HUS) in Germany, including 90 children. Data on the intermediate outcome in children after HUS due to E. coli O104:H4 have been lacking. Methods.: Follow-up data were gathered retrospectively from the medical records of patients who had been included in the German Pediatric HUS Registry during the 2011 outbreak. Results.: A total of 72/89 (81%) of the patients was included after a median follow-up of 3...
March 13, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28320988/characterization-of-shiga-toxin-producing-escherichia-coli-from-feces-of-sika-deer-cervus-nippon-in-japan-using-pcr-binary-typing-analysis-to-evaluate-their-potential-human-pathogenicity
#3
Hidenori Kabeya, Shingo Sato, Shinya Oda, Megumi Kawamura, Mariko Nagasaka, Masanari Kuranaga, Eiji Yokoyama, Shinichiro Hirai, Atsushi Iguchi, Tomoe Ishihara, Toshiro Kuroki, Tomoko Morita-Ishihara, Sunao Iyoda, Jun Terajima, Makoto Ohnishi, Soichi Maruyama
This study examined the potential pathogenicity of Shiga toxin-producing Escherichia coli (STEC) in feces of sika deer by PCR binary typing (P-BIT), using 24 selected STEC genes. A total of 31 STEC strains derived from sika deer in 6 prefectures of Japan were O-serotyped and found to be O93 (n=12), O146 (n=5), O176 (n=3), O130 (n=3), O5 (n=2), O7 (n=1), O96 (n=1), O116 (n=1), O141 (n=1), O157 (n=1) and O-untypable (n=1). Of the 31 STEC strains, 13 carried both stx1 and stx2, 5 carried only stx1, and 13 carried one or two variants of stx2...
March 18, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28317440/potent-immune-responses-induced-by-a-salmonella-ghost-delivery-system-that-expresses-the-recombinant-stx2eb-fedf-and-feda-proteins-of-the-escherichia-coli-producing-f18-and-shiga-toxin-in-a-murine-model-and-evaluation-of-its-protective-effect-as-a-porcine-vaccine
#4
Gayeon Won, John Hwa Lee
BACKGROUND: In the pathogenicity of porcine edema disease, which is caused by the Escherichia coli-producing F18 and Shiga toxin, F18(+) fimbrial adhesins and Shiga toxin 2e (Stx2e) play pivotal roles in the colonization and enterotoxicity of this pathogen. OBJECTIVE: To develop a vaccine candidate against edema disease by combining three selected antigens of F18(+) Shiga toxin-producing Escherichia coli (STEC). METHODS: Genetically engineered Salmonella Typhimurium (ST) ghosts that express Stx2eB, FedF, and FedA were individually inserted in a ghost plasmid cassette (pJHL184), and the resultant plasmids were transformed into a Δasd Δlon ΔcpxR ST (JOL912)...
March 20, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28316592/influence-of-stress-factors-related-to-cheese-making-process-and-to-stec-detection-procedure-on-the-induction-of-stx-phages-from-stec-o26-h11
#5
Ludivine Bonanno, Benjamin Delubac, Valérie Michel, Frédéric Auvray
Shiga toxin-producing Escherichia coli (STEC) are responsible for human infections, ranging from mild watery diarrhea to hemorrhagic colitis (CH) that may be complicated by hemolytic uremic syndrome (HUS). The main STEC virulence factor is Shiga toxin encoded by the stx gene, located in the genome of a bacteriophage integrated into the bacterial chromosome. The serotype O26:H11 is the second HUS-causing serotype worldwide (after O157:H7), and the first found in dairy products such as raw-milk cheeses. A small number of HUS cases identified each year in France are caused by serotype O26:H11...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28316051/pathogenic-multiple-antimicrobial-resistant-escherichia-coli-serotypes-in-recreational-waters-of-mumbai-india-a-potential-public-health-risk
#6
Aayushi Maloo, Abhay B Fulke, Najmuddin Mulani, Soniya Sukumaran, Anirudh Ram
Globally, coastal waters have emerged into a pool of antibiotic resistance genes and multiple antibiotic resistant microorganisms, and pathogenicity of these resistant microorganisms in terms of serotypes and virulence genes has made the environment vulnerable. The current study underscores the presence of multiple antibiotic resistant pathogenic serotypes and pathotypes of Escherichia coli, the predominant faecal indicator bacteria (FIB), in surface water and sediment samples of famous recreational beaches (Juhu, Versova, Mahim, Dadar, and Girgaon) of Mumbai...
March 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28304216/characterizing-the-multidrug-resistance-of-non-o157-shiga-toxin-producing-escherichia-coli-isolates-from-cattle-farms-and-abattoirs
#7
Carrie-Ann Kennedy, Séamus Fanning, Maria Karczmarczyk, Brian Byrne, Áine Monaghan, Declan Bolton, Torres Sweeney
Non-O157 Shiga toxin-producing Escherichia coli (STECs) are not as well characterized as O157 STEC cases, despite their similar prevalence in many countries. Hence, the objective of this study was to investigate the phenotypic and genotypic basis of multidrug resistance (MDR) in non-O157 STEC farm- and abattoir-sourced isolates and assess the potential dissemination of these MDR profiles in vitro. Susceptibility testing to 20 antimicrobials was performed on 146 non-O157 STECs isolated from farm and abattoir environments...
March 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28303131/multiplexed-single-intact-cell-droplet-digital-pcr-music-ddpcr-method-for-specific-detection-of-enterohemorrhagic-e-coli-ehec-in-food-enrichment-cultures
#8
Tanis C McMahon, Burton W Blais, Alex Wong, Catherine D Carrillo
Foodborne illness attributed to enterohemorrhagic E. coli (EHEC), a highly pathogenic subset of Shiga toxin-producing E. coli (STEC), is increasingly recognized as a significant public health issue. Current microbiological methods for identification of EHEC in foods often use PCR-based approaches to screen enrichment broth cultures for characteristic gene markers [i.e., Shiga toxin (stx) and intimin (eae)]. However, false positives arise when complex food matrices, such as beef, contain mixtures of eae-negative STEC and eae-positive E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28294687/twenty-two-years-of-u-s-meat-and-poultry-product-recalls-implications-for-food-safety-and-food-waste
#9
Acton Gorton, Matthew J Stasiewicz
The U.S. Department of Agriculture, Food Safety and Inspection Service maintains a recall case archive of meat and poultry product recalls from 1994 to the present. In this study, we collected all recall records from 1994 to 2015 and extracted the recall date, meat or poultry species implicated, reason for recall, recall class, and pounds of product recalled and recovered. Of a total of 1,515 records analyzed, the top three reasons for recall were contamination with Listeria, undeclared allergens, and Shiga toxin-producing Escherichia coli ...
March 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28294683/evaluation-of-rectoanal-mucosal-swab-sampling-for-molecular-detection-of-enterohemorrhagic-escherichia-coli-in-beef-cattle
#10
Getahun E Agga, Terrance M Arthur, Susanne Hinkley, Joseph M Bosilevac
Cattle are a primary reservoir of enterohemorrhagic Escherichia coli (EHEC), and contaminated beef products are a source of human infections. The U.S. Department of Agriculture Food Safety and Inspection Service declared seven EHEC serogroups (O26, O45, O103, O111, O121, O145, and O157) as adulterants in raw ground beef. Sampling a large number of animals for EHEC surveillance or evaluations of EHEC-focused preharvest interventions requires a convenient and robust sampling method. We evaluated the diagnostic performance of rectoanal mucosal swab (RAMS) for the detection of the top seven EHEC serogroups...
March 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28294553/nitric-oxide-enhanced-shiga-toxin-production-was-regulated-by-fur-and-reca-in-enterohemorrhagic-escherichia-coli-o157
#11
Kimitoshi Ichimura, Takeshi Shimizu, Akio Matsumoto, Shinichiro Hirai, Eiji Yokoyama, Hiroki Takeuchi, Kinnosuke Yahiro, Masatoshi Noda
Enterohemorrhagic Escherichia coli (EHEC) produces Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2). Nitric oxide (NO), which acts as an antimicrobial defense molecule, was found to enhance the production of Stx1 and Stx2 in EHEC under anaerobic conditions. Although EHEC O157 has two types of anaerobic NO reductase genes, an intact norV and a deleted norV, in the deleted norV-type EHEC, a high concentration of NO (12-29 μmol/L, maximum steady-state concentration) is required for enhanced Stx1 production and a low concentration of NO (~12 μmol/L, maximum steady-state concentration) is sufficient for enhanced Stx2 production under anaerobic conditions...
March 15, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28292660/prevalence-antimicrobial-resistance-and-multiple-locus-variable-number-tandem-repeat-analysis-profiles-of-diarrheagenic-escherichia-coli-isolated-from-different-retail-foods
#12
Lili Wang, Hiromi Nakamura, Eriko Kage-Nakadai, Yukiko Hara-Kudo, Yoshikazu Nishikawa
Diarrheagenic E. coli (DEC) isolates were recovered from local retail markets and the Osaka Municipal Central Wholesale Market in Japan. Retail food samples were collected for analysis in Osaka Japan from 2005 to 2008 and consisted of 32 beef, 28 pork, 20 poultry, 136 fish, 66 fruits and vegetables and 51 ready-to-eat (RTE) food samples. A total of 82 DEC strains were recovered from 64 (19%) food samples with the highest prevalence in poultry (100%, 20/20), followed by pork (54%, 15/28), beef (28%, 9/32), fruits and vegetables (12%, 8/66), fish (6...
March 7, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28287277/inhibitory-potential-of-strepthonium-a-against-shiga-toxin-production-in-enterohemorrhagic-escherichia-coli-ehec-strain-edl933
#13
Cheng Cheng, Srikkanth Balasubramanian, Agnes Fekete, Markus Krischke, Martin J Mueller, Ute Hentschel, Tobias A Oelschlaeger, Usama Ramadan Abdelmohsen
The production of shiga toxin (Stx) is a critical step in the establishment and progress of enterohemorrhagic Escherichia coli (EHEC) infections. The possible release of Stx from dead and dying bacteria, and the risk of resistance development have restricted the usage of antibiotics against EHEC. The chlorinated quaternary ammonium compound, strepthonium A, was isolated from the culture of Streptomyces sp. SBT345 that was cultivated from the Mediterranean sponge Agelas oroides. The structure was elucidated and confirmed by spectroscopic analyses including 1D and 2D NMR, ESI-HRMS, as well as ESI-HRMS(2)...
March 13, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28281260/low-cost-charged-coupled-device-ccd-based-detectors-for-shiga-toxins-activity-analysis
#14
Reuven Rasooly, Ben Prickril, Hugh A Bruck, Avraham Rasooly
To improve food safety there is a need to develop simple, low-cost sensitive devices for detection of food-borne pathogens and their toxins. We describe a simple, low-cost webcam-based detector which can be used for various optical detection modalities, including fluorescence, chemiluminescence, densitometry, and colorimetric assays. The portable battery-operated CCD-based detection system consists of four modules: (1) a webcam to measure and record light emission, (2) a sample plate to perform assays, (3) a light emitting diode (LED) for illumination, and (4) a portable computer to acquire and analyze images...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28278412/revised-surveillance-case-definitions-shiga-toxin-producing-escherichia-coli-stec
#15
(no author information available yet)
No abstract text is available yet for this article.
September 30, 2016: Communicable Diseases Intelligence Quarterly Report
https://www.readbyqxmd.com/read/28274429/pectic-oligosaccharide-structure-function-relationships-prebiotics-inhibitors-of-escherichia-coli-o157-h7-adhesion-and-reduction-of-shiga-toxin-cytotoxicity-in-ht29-cells
#16
Rong Di, Malathi S Vakkalanka, Chatchaya Onumpai, Hoa K Chau, Andre White, Robert A Rastall, Kit Yam, Arland T Hotchkiss
Shiga toxin (Stx)-producing, food-contaminating Escherichia coli (STEC) is a major health concern. Plant-derived pectin and pectic-oligosaccharides (POS) have been considered as prebiotics and for the protection of humans from Stx. Of five structurally different citrus pectic samples, POS1, POS2 and modified citrus pectin 1 (MCP1) were bifidogenic with similar fermentabilities in human faecal cultures and arabinose-rich POS2 had the greatest prebiotic potential. Pectic oligosaccharides also enhanced lactobacilli growth during mixed batch faecal fermentation...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28260536/nationwide-investigation-of-shiga-toxin-producing-escherichia-coli-among-cattle-in-japan-revealed-the-risk-factors-and-potentially-virulent-subgroups
#17
K Lee, M Kusumoto, T Iwata, S Iyoda, M Akiba
A nationwide study of Shiga toxin-producing Escherichia coli (STEC) was performed to determine the prevalence, characteristics and risk factors for fecal shedding of STEC among cattle in Japan. Information on rearing practices was also collected to identify risk factors for fecal shedding of STEC. STEC was isolated from 24·1% of samples (133/551) collected from 59·1% of farms (65/110). Bayesian clustering using the virulence marker profiles of the isolates subdivided the isolates into four genetically distinct groups, two of which corresponded to eae- or saa-positive STEC, which can cause severe disease in human...
March 6, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28254967/draft-genome-sequence-of-five-shiga-toxin-producing-escherichia-coli-strains-isolated-from-wild-deer-in-japan
#18
Hiroshi Asakura, Tetsuya Ikeda, Shiori Yamamoto, Hidenori Kabeya, Hiromu Sugiyama, Shinji Takai
Shiga toxin-producing Escherichia coli (STEC) is one of the major foodborne pathogens. Having observed the wide distribution of this pathogen in wild deer, we report here the draft genome sequence of five STEC strains isolated from wild deer (Cervus nippon yesoensis) in Hokkaido, Japan.
March 2, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28248675/occurrence-of-plasmid-mediated-quinolone-resistance-and-virulence-genes-in-avian-escherichia-coli-isolates-from-algeria
#19
Meradi Laarem, Abouddihaj Barguigua, Kaotar Nayme, Abdi Akila, Khalid Zerouali, Naima El Mdaghri, Mohammed Timinouni
INTRODUCTION: The emergence and spread of quinolone-resistant Escherichia coli in poultry products puts consumers at risk of exposure to the strains of E. coli that resist antibiotic treatment. The objective of this study was to define the prevalence and virulence potential of poultry-associated nalidixic acid (NAL)-resistant E. coli in the Annaba city, Algeria. METHODOLOGY: In total, 33 samples of retail chicken meat were purchased from various butcher shops and examined for bacterial contamination with NAL-resistant E...
February 28, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28242109/haemolytic-uraemic-syndrome
#20
REVIEW
Fadi Fakhouri, Julien Zuber, Véronique Frémeaux-Bacchi, Chantal Loirat
Haemolytic uraemic syndrome is a form of thrombotic microangiopathy affecting predominantly the kidney and characterised by a triad of thrombocytopenia, mechanical haemolytic anaemia, and acute kidney injury. The term encompasses several disorders: shiga toxin-induced and pneumococcus-induced haemolytic uraemic syndrome, haemolytic uraemic syndrome associated with complement dysregulation or mutation of diacylglycerol kinase ɛ, haemolytic uraemic syndrome related to cobalamin C defect, and haemolytic uraemic syndrome secondary to a heterogeneous group of causes (infections, drugs, cancer, and systemic diseases)...
February 23, 2017: Lancet
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