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https://www.readbyqxmd.com/read/28944894/glucosylceramide-synthase-regulates-the-proliferation-and-apoptosis-of-liver-cells-in%C3%A2-vitro-by-bcl%C3%A2-2-bax-pathway
#1
Jun-Feng Li, Su-Jun Zheng, Li-Li Wang, Shuang Liu, Feng Ren, Yu Chen, Li Bai, Mei Liu, Zhong-Ping Duan
Our previous study found that glucosylceramide, a type of sphingolipids, was associated with liver inflammation and fibrosis. Glucosylceramide is generated by glucosylceramide synthase (GCS), which is encoded by the UDP‑glucose ceramide glucosyltransferase (UGCG) gene. GCS is a key enzyme to regulate the physiological activity of cells. However, the role of GCS in hepatic cells remains unclear. The aim of the present study was to explore the mechanism of GCS in the proliferation and apoptosis of liver cells...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944890/paeonol-exerts-potential-activities-to-inhibit-the-growth-migration-and-invasion-of-human-gastric-cancer-bgc823-cells-via-downregulating-mmp%C3%A2-2-and-mmp%C3%A2-9
#2
Zhong-Kuan Lyu, Chang-Ling Li, Yan Jin, Yu-Zhao Liu, Xi Zhang, Fang Zhang, Lu-Ning Ning, Er-Shun Liang, Min Ma, Wei Gao, Ming-Xiang Zhang, De-Shan Liu
Paeonol (Pae) is an herbal extract that has attracted extensive attention for its anti‑cancer effects demonstrated by a number of studies, which have predominantly demonstrated inhibition of cell proliferation and induction of apoptosis. The influence of Pae on cancer cell metastasis has been less widely reported. The present study aimed to investigate the under‑reported effects of Pae on the growth, invasion and migration of poorly differentiated BGC823 gastric cancer cells with strong invasive and metastatic abilities...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944156/peripheral-ischaemic-retinopathy-and-neovascularisation-in-a-patient-with-subacute-streptococcus-mitis-induced-bacterial-endocarditis
#3
Laura S Leysen, Elke O Kreps, Ilse De Schryver, Kristien P Hoornaert, Vanessa Smith, Julie De Zaeytijd
Objective: To describe a patient with peripheral retinal ischaemia and neovascularisation who was diagnosed with streptococcus mitis-induced bacterial endocarditis. Methods: Retrospective analysis of case report. A 57-year-old man presented with a history of a rapidly progressive, bilateral, painless visual loss. He also suffered from pain in the neck and lower back and a weight loss of 10 kg. He underwent a full ophthalmologic work-up, laboratory investigations, and imaging of the spine. Results: BCVA was reduced to 20/40 in the right eye and 20/32 in the left eye...
2017: GMS Ophthalmology Cases
https://www.readbyqxmd.com/read/28944153/sequential-development-of-non-arteritic-anterior-ischemic-optic-neuropathy-in-a-patient-on-hemodialysis
#4
Mukesh Jain, Renuka Srinivasan, K Ramesh Babu, M Swapnil Parchand
Purpose: Non-arteritic anterior ischemic optic neuropathy (NA-AION) is typically a disorder of patients 50 years and older predisposed to the vascular and structural optic disc risk factors. We present an interesting case of sequential development of NA-AION in a 45-year-old patient with end-stage renal disease undergoing hemodialysis. Methods: Observational case report. Results: A 45-year-old female on hemodialysis for chronic renal failure complained of sequential acute onset sudden painless gross diminution of vision in right eye followed by the left eye...
2017: GMS Ophthalmology Cases
https://www.readbyqxmd.com/read/28943944/andrographolide-suppresses-proliferation-of-human-colon-cancer-sw620-cells-through-the-tlr4-nf-%C3%AE%C2%BAb-mmp-9-signaling-pathway
#5
Rui Zhang, Jian Zhao, Jian Xu, De-Xin Jiao, Jian Wang, Zhi-Qiang Gong, Jian-Hui Jia
Modern pharmacological research has revealed that andrographolide has various functions, including anti-bacterial, anti-inflammatory and anti-viral effects, immunoregulation, treating cardiovascular and cerebrovascular diseases, and prevention and treatment of alcoholic liver injury. The present study investigated whether andrographolide suppresses the proliferation of human colon cancer cell through the Toll-like receptor 4 (TLR4)/nuclear factor (NF)-κB/matrix metalloproteinase-9 (MMP-9) signaling pathway...
October 2017: Oncology Letters
https://www.readbyqxmd.com/read/28943017/comparison-of-subjective-grading-of-lid-wiper-epitheliopathy-with-a-semi-objective-method
#6
Carolina M E Kunnen, James S Wolffsohn, Eric R Ritchey
PURPOSE: To validate a semi-objective method of grading lid wiper epitheliopathy (LWE) compared to subjective assessment. METHODS: Twenty upper and 20 lower eyelid margins of patients with LWE were photographed after instillation of fluorescein and lissamine green. The images were graded by two observers using a 0-3 grading scale for height (%) and width (mm) of the lid staining. The images were also processed using custom designed software in MATLAB. After manual delineation of the staining area, width and perpendicular height were automatically measured throughout the selected area...
September 21, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28941304/collagen-agarose-alginate-and-matrigel-hydrogels-as-cell-substrates-for-culture-of-chondrocytes-in-vitro-a-comparative-study
#7
Zhikang Miao, Zhenhui Lu, Huayu Wu, Hui Liu, Muyan Li, Danqing Lei, Li Zheng, Jinmin Zhao
Autologous chondrocyte implantation (ACI) has emerged as a new approach to cartilage repair through the use of harvested chondrocytes. But the expansion of the chondrocytes from the donor tissue in vitro is restricted by limited cell numbers and dedifferentiation of chondrocytes. In this study, we used four types of hydrogels including agarose, alginate, Matrigel and collagen type I hydrogels to serve as cell substrates and investigated the effect on proliferation and phenotype maintenance of chondrocytes. As a substrate for monolayer culture, collagen facilitated cell expansion and effectively suppressed the dedifferentiation of chondrocytes, as evidenced by fluorescein diacetate / propidium iodide (FDA/PI), hematoxylin-eosin staining (HE), Safranin O, immunofluorescenceassay, biochemistry analysis and quantitative real-time polymerase chain reaction (qRT-PCR)...
September 23, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28940146/dry-eye-evaluation-and-correlation-analysis-between-tear-film-stability-and-corneal-surface-regularity-after-small-incision-lenticule-extraction
#8
Hui Zhang, Yan Wang
PURPOSE: To investigate the dry eye after small incision lenticule extraction (SMILE) and explore the correlations between changes in the tear film stability, the tear secretion and the corneal surface regularity. METHODS: Sixty-two eyes of 22 men and 13 women who underwent SMILE were included in this study. Corneal topography was measured to assess the index of surface variance (ISV) and the index of vertical asymmetry (IVA). Dry eye tests including subjective symptom questionnaire, tear breakup time (TBUT), corneal fluorescein staining and Schirmer's test (ST) were evaluated before and at 1 and 6 months postoperatively...
September 22, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28939118/dry-eye-disease-in-the-elderly-in-a-french-population-based-study-the-montrachet-study-maculopathy-optic-nerve-nutrition-neurovascular-and-heart-diseases-prevalence-and-associated-factors
#9
Arthur Ferrero, Seydou Alassane, Christine Binquet, Lionel Bretillon, Niyazi Acar, Louis Arnould, Aurore Muselier-Mathieu, Cécile Delcourt, Alain M Bron, Catherine Creuzot-Garcher
PURPOSE: To estimate the prevalence of dry eye disease (DED) in the elderly and to describe systemic and ocular factors associated with DED in a population-based study, the Montrachet (Maculopathy Optic Nerve nuTRition neurovAsCular and HEarT diseases) study. METHODS: Dry eye symptoms were evaluated using the Ocular Surface Disease Index (OSDI) questionnaire. A self-reported history of dry eye symptoms and artificial tear use were also recorded. Every participant underwent an ophthalmic evaluation, which included the Schirmer test without anesthesia (Schirmer I), tear film breakup time (TFBUT) measurement, and fluorescein corneal staining evaluation...
September 19, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28938608/use-of-confocal-laser-endomicroscopy-with-a-fluorescently-labeled-fatty-acid-to-diagnose-colorectal-neoplasms
#10
Feihong Deng, Yuan Fang, Zhiyong Shen, Wei Gong, Tao Liu, Jing Wen, Wanling Zhang, Xianjun Zhu, Hui Zhong, Tong Wang, Fachao Zhi, Biao Nie
Endoscopic treatment for early colorectal cancer closely correlates with patient prognosis. However, endoscopic differentiation between carcinomas and non-neoplastic lesions remains difficult. Here, we topically stained colorectal neoplasms with a fatty acid analogue (BODIPY-FA) and quantified the fluorescent signals using confocal laser endomicroscopy (CLE) and fluorescence microscopy. We also analyzed protein expression in colorectal cancer tissues. We found that expression of fatty acid synthase was elevated, while the expression of fatty acid transporters was reduced in colorectal cancer...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938164/multifunctional-superparamagnetic-nanoparticles-conjugated-with-fluorescein-labeled-designed-ankyrin-repeat-protein-as-an-efficient-her2-targeted-probe-in-breast-cancer
#11
Dong-Li Li, Jian-Er Tan, Ying Tian, Shun Huang, Peng-Hui Sun, Meng Wang, Yan-Jiang Han, Hong-Sheng Li, Hu-Bing Wu, Xing-Mei Zhang, Yi-Kai Xu, Quan-Shi Wang
Based on the discordance of human epidermal growth factor receptor-2 (HER2) expression between primary and metastatic/recurrent breast cancer, HER2 molecular imaging, which had potential to systemically assess and dynamically monitor HER2 expression, might improve the selection of patients for anti-HER2 therapy. In this study, designed ankyrin repeat protein (DARPin) G3, a novel binding protein with picomolar affinity for HER2, was used and multifunctional superparamagnetic nanoparticles modified with fluorescein-5-maleimide-labeled DARPin G3 (SPIO-G3-5MF) were developed for HER2 imaging...
September 8, 2017: Biomaterials
https://www.readbyqxmd.com/read/28937833/oct-angiography-for-monitoring-and-managing-neovascular-age-related-macular-degeneration
#12
P Malamos, G Tsolkas, M Kanakis, G Mylonas, D Karatzenis, N Oikonomopoulos, J Lakoumentas, Ilias Georgalas
PURPOSE: To evaluate the combined use of optical coherence tomography and angiography (OCT-A) for imaging choroidal neovascularization (CNV) secondary to neovascular age-related macular degeneration (nAMD). MATERIALS AND METHODS: This prospective observational study was conducted from May 2015 to April 2017. Included in the study were 54 patients (n = 63 eyes), all of whom had CNV secondary to nAMD and all of whom had been examined by OCT-A. Angioscans (3x3 and 6 × 6) and conventional B-scan OCT scans were obtained for all patients at baseline and at various times during the 24-month follow-up period...
September 22, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28937831/impact-of-smoking-on-the-ocular-surface-tear-function-and-tear-osmolarity
#13
Serdar Aktaş, Mehmet Tetikoğlu, Ahmet Koçak, Metin Kocacan, Hatice Aktaş, Hacı Murat Sağdık, Fatih Özcura
PURPOSE: This study evaluated the effects of cigarette smoking on the ocular surface, tear function, and tear osmolarity. MATERIALS AND METHODS: A total of 50 smokers with at least 5 years of heavy smoking (defined as 1 pack/day) and 51 nonsmoking, healthy individuals were enrolled. Tear osmolarity was measured with an osmometer (TearLab(™) Osmolarity System). Ocular surface examinations involved corneal fluorescein staining, measurement of the tear film breakup time (TBUT), the Schirmer 1 test, measurement of corneal sensitivity with a Cochet-Bonnet esthesiometer, and conjunctival impression cytology...
September 22, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28937826/choroidal-variations-in-diabetic-macular-edema-fluorescein-angiography-and-optical-coherence-tomography
#14
Xiangning Wang, Shuting Li, Wei Li, Yanjun Hua, Qiang Wu
PURPOSES: To (i) evaluate variations in choroidal thickness (CT) and choroidal volume (CV) in diabetic macular edema (DME), as demonstrated by fluorescein angiography (FA) and enhanced depth-imaging (EDI) optical coherence tomography (OCT) (EDI-OCT) and (ii) correlate these variations with visual acuity. METHODS: This study was a retrospective observational case series of patients with diabetic retinopathy (DR) and DME. The study groups comprised 77 DR patients (n = 87), all of whom underwent color fundus photography, FA, and OCT on the same day...
September 22, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28937524/self-inflicted-laser-handheld-laser-induced-maculopathy-a-novel-ocular-manifestation-of-factitious-disorder
#15
Alessandro Rabiolo, Riccardo Sacconi, Chiara Giuffrè, Eleonora Corbelli, Adriano Carnevali, Lea Querques, David Sarraf, K Bailey Freund, SriniVas Sadda, Francesco Bandello, Giuseppe Querques
PURPOSE: To report a case of factitious self-inflicted handheld laser-induced maculopathy. METHODS: A 29-year-old man presented to our clinic complaining of a step-wise progressive loss of vision that abruptly began in his left eye but then became bilateral. He underwent comprehensive ocular examination, including visual acuity testing, biomicroscopic, dilated funduscopic examination, structural optical coherence tomography, en face structural optical coherence tomography, optical coherence tomography angiography, fundus autofluorescence, fluorescein angiography, and indocyanine green angiography...
September 19, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28937523/cytomegalovirus-retinitis-associated-with-occlusive-vasculopathy-in-an-elderly-human-immunodeficiency-virus-negative-man
#16
Kareem Moussa, Thuy Doan, Jay M Stewart, Jessica Shantha, John Gonzales, Nisha Acharya, Emmett T Cunningham
PURPOSE: To present a case of cytomegalovirus (CMV) retinitis associated with occlusive vasculopathy presenting as sudden unilateral loss of vision in a human immunodeficiency virus-negative elderly man. METHODS: Clinical case report and literature review. RESULTS: An 84-year-old Chinese man with diabetes mellitus and primary open-angle glaucoma was seen in consultation by our uveitis service for evaluation of sudden vision loss in the right eye...
September 20, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28937147/comparison-of-effectiveness-and-safety-between-conbercept-and-ranibizumab-for-treatment-of-neovascular-age-related-macular-degeneration-a-retrospective-case-controlled-non-inferiority-multiple-center-study
#17
J Cui, D Sun, H Lu, R Dai, L Xing, H Dong, L Wang, D Wei, B Jiang, Y Jiao, M M Jablonski, S Charles, W Gu, H Chen
PurposeTo compare the efficacy and safety of conbercept and ranibizumab when administered according to a treat-and-extend (TREX) protocol for the treatment of neovascular age-related macular degeneration (AMD) in China.Patients and methodsBetween May 2014 and May 2015, 180 patients were treated in a 1 : 1 ratio using conbercept or ranibizumab from four hospitals. Patients received either conbercept 0.5 mg or ranibizumab 0.5 mg intravitreal injections. Follow-up time was 1 year and treated based on a TREX approach...
September 22, 2017: Eye
https://www.readbyqxmd.com/read/28936058/multifocal-choroiditis-with-retinal-vasculitis-optic-neuropathy-and-keratoconus-in-a-young-saudi-male
#18
Yousef Dhafiri, Khalid Al Rubaie, Omar Kirat, William N May, Quan D Nguyen, Igor Kozak
The purpose of this study is to describe an association of unilateral multifocal choroiditis (MFC), retinal vasculitis, optic neuropathy, and bilateral keratoconus in a young Saudi male. A 27-year-old male patient with stable bilateral keratoconus presented with a painless vision loss in his left eye. Ophthalmic examinations revealed multiple foci of idiopathic chorioretinitis, retinal vasculitis, and mild optic disc leakage on fluorescein angiography, all of which resolved on systemic therapy with mycophenolate mofetil and prednisone after 3 months...
April 2017: Middle East African Journal of Ophthalmology
https://www.readbyqxmd.com/read/28935759/lymphangiogenesis-facilitates-initial-lymph-formation-and-enhances-the-dendritic-cell-mobilizing-chemokine-ccl21-without-affecting-migration
#19
Tine V Karlsen, Tore Reikvam, Anne Tofteberg, Elham Nikpey, Trude Skogstrand, Marek Wagner, Olav Tenstad, Helge Wiig
OBJECTIVE: Lymphatic vessels play an important role in body fluid, as well as immune system homeostasis. Although the role of malfunctioning or missing lymphatics has been studied extensively, less is known on the functional consequences of a chronically expanded lymphatic network or lymphangiogenesis. APPROACH AND RESULTS: To this end, we used K14-VEGF-C (keratin-14 vascular endothelial growth factor-C) transgenic mice overexpressing the vascular endothelial growth factor C in skin and investigated the responses to inflammatory and fluid volume challenges...
September 21, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28935590/evaluation-of-transdermal-drug-permeation-as-modulated-by-lipoderm-and-pluronic-lecithin-organogel
#20
Qian Zhang, Yunmei Song, Stephen W Page, Sanjay Garg
The transdermal delivery of two fluorescent probes with similar molecular weight but different lipophilicity, into and through the skin from two commercially available transdermal bases, pluronic lecithin organogel (PLO) and Lipoderm(®) has been evaluated. First, in vitro penetration of fluorescein sodium and fluorescein (free acid) through porcine skin were evaluated. Retention and depth distribution profiles in skin were obtained by tape-stripping and then followed by optical sectioning using multiphoton microscopy...
September 18, 2017: Journal of Pharmaceutical Sciences
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