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https://www.readbyqxmd.com/read/29334390/smartphone-integrated-optoelectrowetting-sioew-for-on-chip-sample-processing-and-microscopic-detection-of-water-quality
#1
Dongyue Jiang, Seunguk Lee, Sung Woo Bae, Sung-Yong Park
With the increasing capabilities and ubiquity of smartphones and their associated digital cameras, this study presents a smartphone integrated optoelectrowetting (SiOEW) device as a simple, portable tool capable of on-chip water sample preparation and microscopic detection of the target cells in water samples, which significantly reduce the detection time and the labor cost required for water quality monitoring. A commercially available smartphone is used as a low-intensity portable light source to perform optoelectrowetting (OEW)-based microfluidic operations such as droplet transportation, merging, mixing, and immobilization on a hydrophobic detection zone...
January 15, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29332275/impact-of-drought-stress-induced-by-polyethylene-glycol-on-growth-water-relations-and-cell-viability-of-norway-spruce-seedlings
#2
Ilya E Zlobin, Yury V Ivanov, Alexander V Kartashov, Vladimir V Kuznetsov
We investigated physiological responses of 7-week-old Norway spruce seedlings to water deficits of different intensities. Hydroponically grown seedlings were subjected to mild (-0.15 MPa), strong (-0.5 and -1.0 MPa) and extreme (-1.5 MPa) water deficit induced by polyethylene glycol 6000, and their growth parameters, water status and physiological activity were analyzed. Seedlings effectively restricted water loss under drought, and even under extreme water deficit, shoot relative water content did not fall below 85%...
January 5, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29330463/effective-photo-enhancement-of-cellular-activity-of-fluorophore-octaarginine-antisense-pna-conjugates-correlates-with-singlet-oxygen-formation-endosomal-escape-and-chromophore-lipophilicity
#3
Reza Yarani, Takehiko Shiraishi, Peter E Nielsen
Photochemical internalization (PCI) is a cellular drug delivery method based on the generation of light-induced reactive oxygen species (ROS) causing damage to the endosomal membrane and thereby resulting in drug release to the cytoplasm. In our study a series of antisense fluorophore octaarginine peptide nucleic acid (PNA) conjugates were investigated in terms of PCI assisted cellular activity. It is found that tetramethylrhodamine and Alexa Fluor 555 conjugated octaarginine PNA upon irradiation exhibit more than ten-fold increase in antisense activity in the HeLa pLuc705 luciferase splice correction assay...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29328450/mybl2-protects-against-h9c2-injury-induced-by-hypoxia-via-akt-and-nf%C3%A2-%C3%AE%C2%BAb-pathways
#4
Mingfeng Shao, Zexiang Ren, Rongjun Zhang
Cardiovascular diseases have become one of the major public health problems in many countries. The downregulation of MYBL2 was found in H9c2 and native cardiomyocytes cells after hypoxia treatment. The present study aimed to investigate the effects of MYB proto‑oncogene like 2 (MYBL2) on H9c2 injury induced by hypoxia. Reverse transcription‑quantitative polymerase chain reaction and western blot were performed on H9c2 cells to determine the mRNA and protein levels of MYBL2, respectively. Small interfering RNA (siRNA) was employed to downregulate MYBL2 expression in H9c2 cells to investigate changes in cell proliferation and apoptosis...
January 8, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328378/liposome%C3%A2-delivered-baicalein-induction-of-myeloid-leukemia-k562-cell-death-via-reactive-oxygen-species-generation
#5
Scarlet Xiaoyan Wang, Xuesong Wen, Celia Bell, Sandra Appiah
Baicalein (BL), a potential cancer chemopreventative flavone, has been reported to inhibit cancer cell growth by inducing apoptosis and causing cell cycle arrest in various human cancer cell models. Delivery of BL via nanoliposomes has been shown to improve its oral bioavailability and long‑circulating property in vivo. However, the role of BL in the inhibition of human chronic myeloid leukemia (CML) K562 cell growth and its underlying mechanisms has yet to be elucidated. In the present study, BL was formulated into liposomes with different sizes to improve its solubility and stability...
January 8, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29327400/a-vitamin-b12-conjugate-of-exendin-4-improves-glucose-tolerance-without-associated-nausea-or-hypophagia-in-rodents
#6
Elizabeth G Mietlicki-Baase, Claudia G Liberini, Jayme L Workinger, Ron L Bonaccorso, Tito Borner, David J Reiner, Kieran Koch-Laskowski, Lauren E McGrath, Rinzin Lhamo, Lauren M Stein, Bart C De Jonghe, George G Holz, Christian L Roth, Robert P Doyle, Matthew R Hayes
AIMS: While pharmacological glucagon-like peptide-1 receptor (GLP-1R) agonists are FDA-approved for treating type 2 diabetes mellitus (T2DM) and obesity, a major side effect is nausea/malaise. We recently developed a conjugate of vitamin B12 bound to the GLP-1R agonist exendin-4 (Ex4), which displays enhanced proteolytic stability and retention of GLP-1R agonism. Here, we evaluate whether the conjugate (B12-Ex4) can improve glucose tolerance without producing anorexia and malaise. MATERIALS AND METHODS: We evaluated the effects of systemic B12-Ex4 and unconjugated Ex4 on food intake and body weight change, oral glucose tolerance, and nausea/malaise in male rats, and on intraperitoneal glucose tolerance in mice...
January 12, 2018: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/29326855/all-types-of-age-related-macular-degeneration-in-one-patient
#7
Zafer Cebeci, Nur Kır
Herein, we describe a neovascular age-related macular degeneration patient with retinal angiomatous proliferation (RAP) and polypoidal choroidal vasculopathy (PCV) coexisting in the same eye at the time of diagnosis. A 55-year-old woman presented with a history of decreased vision in her left eye. Fundoscopy, fluorescein and indocyanine green angiography, and optical coherence tomography imaging revealed RAP and PCV lesions in her left eye at first diagnosis. The patient received intravitreal ranibizumab therapy but developed tachyphylaxis after the first dose despite having three monthly doses...
December 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29324624/intravitreal-aflibercept-in-the-treatment-of-polypoidal-choroidal-vasculopathy-associated-with-morning-glory-syndrome
#8
Claudio Iovino, Maurizio Fossarello, Enrico Peiretti
PURPOSE: To describe an unusual case of polypoidal choroidal vasculopathy secondary to morning glory syndrome successfully treated with three aflibercept intravitreal injections. METHODS: Case report. RESULTS: A 68-year-old white man presented with a 2-month history of diminished vision of his left eye. Fundus examination showed a morning glory syndrome disk anomaly with some perimacular subretinal hemorrhages and lipid depositions. Fundus autofluorescence, fluorescein and green indocyanine angiography, spectral domain optical coherence tomography, and optical coherence tomography angiography were performed and confirmed the presence of a juxtapapillary polypoidal choroidal vasculopathy with intraretinal and subretinal fluid...
January 10, 2018: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/29324593/incidence-and-long-term-visual-acuity-outcomes-of-retinal-pigment-epithelium-tears-after-intravitreal-anti-vascular-endothelial-growth-factor-treatment-of-neovascular-age-related-macular-degeneration
#9
Clara Vazquez-Alfageme, Luke Nicholson, Robin D Hamilton, Praveen J Patel
PURPOSE: To report the incidence of retinal pigment epithelium tears in eyes treated with aflibercept for neovascular age-related macular degeneration and compare it with ranibizumab, and to describe long-term visual outcomes of retinal pigment epithelium tears after intensive anti-vascular endothelial growth factor treatment. METHODS: Retrospective analysis of clinical charts, spectral domain optical coherence tomography and fundus fluorescein angiography imaging of consecutive naive patients treated with intravitreal aflibercept or ranibizumab for neovascular age-related macular degeneration...
January 10, 2018: Retina
https://www.readbyqxmd.com/read/29323486/a-ph-sensing-optode-for-mapping-spatiotemporal-gradients-in-3d-paper-based-cell-cultures
#10
Rachael M Kenney, Matthew W Boyce, Nathan Whitman, Brenden P Kromhout, Matthew R Lockett
Paper-based cultures are an emerging platform for preparing 3D tissue-like structures. Chemical gradients can be imposed upon these cultures, generating microenvironments similar to those found in poorly vascularized tumors. There is increasing evidence that the tumor microenvironment is responsible for promoting drug resistance and increased invasiveness. Acidosis, or the acidification of the extracellular space, is particularly important in promoting these aggressive cancer phenotypes. To better understand how cells respond to acidosis there is a need for 3D culture platforms that not only model relevant disease states, but also contain sensors capable of quantifying the extracellular environment...
January 11, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29321438/vogt-koyanagi-harada-disease-associated-with-influenza-a-virus-infection-a-case-report
#11
Nozomu Yoshino, Ai Kawamura, Akira Ishii, Katsuyuki Yoshida, Tamami Watanabe, Takeshi Yamashita, Takahiko Fukuchi, Fumihiko Toyoda, Akihiko Kakehashi, Hitoshi Sugawara
We herein report a case of a 31-year-old Japanese man who simultaneously had a positive influenza A virus antigen test result and Vogt-Koyanagi-Harada disease (VKHD), demonstrated by both diffuse multiple early hyperfluorescent points on fluorescein fundus photography and serous retinal detachments on optical coherence tomography. He had meningitis. It was difficult to determine whether the main cause of meningitis was influenza A or VKHD. After initial treatment with peramivir for influenza A and then methylprednisolone pulse with subsequent corticosteroid therapy for VKHD, his symptoms improved gradually...
January 11, 2018: Internal Medicine
https://www.readbyqxmd.com/read/29318471/aav-mediated-gene-delivery-of-the-calreticulin-anti-angiogenic-domain-inhibits-ocular-neovascularization
#12
Leilei Tu, Jiang-Hui Wang, Veluchamy A Barathi, Selwyn M Prea, Zheng He, Jia Hui Lee, James Bender, Anna E King, Grant J Logan, Ian E Alexander, Youn-Shen Bee, Ming-Hong Tai, Gregory J Dusting, Bang V Bui, Jingxiang Zhong, Guei-Sheung Liu
Ocular neovascularization is a common pathological feature in diabetic retinopathy and neovascular age-related macular degeneration that can lead to severe vision loss. We evaluated the therapeutic efficacy of a novel endogenous inhibitor of angiogenesis, the calreticulin anti-angiogenic domain (CAD180), and its functional 112-residue fragment, CAD-like peptide 112 (CAD112), delivered using a self-complementary adeno-associated virus serotype 2 (scAAV2) in rodent models of oxygen-induced retinopathy and laser-induced choroidal neovascularization...
January 9, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29318095/green-and-red-fluorescent-dyes-for-translational-applications-in-imaging-and-sensing-analytes-a-dual-color-flag
#13
REVIEW
Elisabete Oliveira, Emilia Bértolo, Cristina Núñez, Viviane Pilla, Hugo M Santos, Javier Fernández-Lodeiro, Adrian Fernández-Lodeiro, Jamila Djafari, José Luis Capelo, Carlos Lodeiro
Red and green are two of the most-preferred colors from the entire chromatic spectrum, and red and green dyes are widely used in biochemistry, immunohistochemistry, immune-staining, and nanochemistry applications. Selective dyes with green and red excitable chromophores can be used in biological environments, such as tissues and cells, and can be irradiated with visible light without cell damage. This critical review, covering a period of five years, provides an overview of the most-relevant results on the use of red and green fluorescent dyes in the fields of bio-, chemo- and nanoscience...
January 2018: ChemistryOpen
https://www.readbyqxmd.com/read/29318092/green-and-red-fluorescent-dyes-for-translational-applications-in-imaging-and-sensing-analytes-a-dual-color-flag
#14
Elisabete Oliveira, Emilia Bértolo, Cristina Núñez, Viviane Pilla, Hugo M Santos, Javier Fernández-Lodeiro, Adrian Fernández-Lodeiro, Jamila Djafari, José Luis Capelo, Carlos Lodeiro
Invited for this month's cover picture is the BIOSCOPE group of Professors Carlos Lodeiro and José Luis Capelo at the REQUIMTE/UCIBIO-LAQv-FCT University NOVA of Lisbon (Portugal), and their collaborators. The cover picture is devoted to Translational Research, and shows the Portuguese Flag represented by the interaction between cells and Janus gold/silver nanoparticles functionalized with rhodamine (red) and Fluorescein (green) dyes as tools for biomedical translational research. Read the full text of their Review at 10...
January 2018: ChemistryOpen
https://www.readbyqxmd.com/read/29317727/enhanced-nasal-drug-delivery-efficiency-by-increasing-mechanical-loading-using-hypergravity
#15
Dongjoo Kim, Young Hyo Kim, Soonjo Kwon
Nasal route drug administration for local and systemic delivery of many therapeutics has received attention because the nasal cavity is highly vascularized and provides a large surface area for drug absorption. However, nasal mucosa exhibits limited permeability to polar molecules. In this study, we developed a novel method for improving absorption efficiency of polar drugs by applying hypergravity. RPMI 2650 cells and primary human nasal epithelial cells were exposed three times to a 20 min hypergravitational condition (10 × g) with a 20 min rest period after each exposure...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29315861/encapsulation-of-dual-emitting-fluorescent-magnetic-nanoprobe-in-metal-organic-frameworks-for-ultrasensitive-ratiometric-detection-of-cu2
#16
Jing Wang, Haiyong Chen, Fan Ru, Zhen Zhang, Xiang Mao, Duoliang Shan, Jing Chen, Xiaoquan Lu
An effective dual-emission fluorescent metal-organic frameworks (MOFs)-based nanoprobe has been enstablished for ultrasensitive and rapid ratiometric detection of Cu2+. Such nanoprobe was prepared by encapsulating the fluorescein isothiocyanate (FITC)-and Eu(III) complex-functionalized Fe3O4 into the zeolitic imidazolate framework materials(ZIF-8). In this nanoprobe, FITC was used as a reference signal, thus improvingthe influence of external uncertainties. Eu-complex signal could be quenched after adding amount of Cu2+...
January 6, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29315532/treatment-of-mcpt8dtr-mice-with-high-or-low-dose-diphtheria-toxin-leads-to-differential-depletion-of-basophils-and-granulocyte-macrophage-progenitors
#17
Carole El Hachem, Pierre Hener, Peggy Kirstetter, Jiagui Li, Susan Chan, Mei Li
Basophils have been recently recognized to play important roles in type 2 immune responses during allergies and parasitic infection, largely due to the development of novel tools for the in vivo study of these cells. As such, the genetically-engineered MCPT8DTR mouse line has been used to specifically deplete basophils following treatment with diphtheria toxin (DT). In this study, we showed that DT-injected MCPT8DTR mice exhibited a striking decrease of eosinophils and neutrophils in skin when subjected to a hapten fluorescein isothiocyanate (FITC)-induced allergic contact dermatitis (ACD) experimental protocol...
January 8, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29315248/a-red-emitting-multidimensional-sensor-for-the-simultaneous-cellular-imaging-of-biothiols-and-phosphate-ions
#18
Pilar Herrero-Foncubierta, Jose M Paredes, Maria D Giron, Rafael Salto, Juan M Cuerva, Delia Miguel, Angel Orte
The development of new fluorescent probes for cellular imaging is currently a very active field because of the large potential in understanding cell physiology, especially targeting anomalous behaviours due to disease. In particular, red-emitting dyes are keenly sought, as the light in this spectral region presents lower interferences and a deeper depth of penetration in tissues. In this work, we have synthesized a red-emitting, dual probe for the multiplexed intracellular detection of biothiols and phosphate ions...
January 9, 2018: Sensors
https://www.readbyqxmd.com/read/29310428/modulation-of-electroosmotic-flow-through-skin-effect-of-poly-amidoamine-dendrimers
#19
Hye Ji Kim, Seaung Youl Oh
The objective of this work is to evaluate the effect of polyamidoamine (PAMAM) dendrimers on electroosmotic flow (EOF) through skin. The effect of size and concentration of dendrimer was studied, using generation 1, 4 and 7 dendrimer (G1, G4 and G7, respectively). As a marker molecule for the direction and magnitude of EOF, a neutral molecule, acetoaminophen (AAP) was used. The visualization of dendrimer permeation into the current conducting pore (CCP) of skin was made using G4-fluorescein isothiocyanate (FITC) conjugate and confocal microscopy...
January 9, 2018: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/29310316/fluorescein-diacetate-fda-and-its-analogue-as-substrates-for-pi-class-glutathione-s-transferase-gstp1-and-their-biological-application
#20
Yuuta Fujikawa, Taiki Nampo, Masaya Mori, Manami Kikkawa, Hideshi Inoue
Pi class glutathione S-transferase (GSTP1) is highly expressed in various cancerous cells and pre-neoplastic legions, where it is involved in apoptotic resistance or metabolism of several anti-tumour chemotherapeutics. Therefore, GSTP1 is a marker of malignant and pre-malignant cells and is a promising target for visualization and drug development. Here we demonstrate that fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human GSTP1 (hGSTP1) selectively among various cytosolic GSTs...
March 1, 2018: Talanta
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