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https://www.readbyqxmd.com/read/28221924/reductions-in-natural-microbial-flora-nonpathogenic-escherichia-coli-and-pathogenic-salmonella-on-jalapeno-peppers-processed-in-a-commercial-antimicrobial-cabinet-a-pilot-plant-trial
#1
Jeremy M Adler, Erin D Cain-Helfrich, Cangliang Shen
This experiment aimed to validate the use of antimicrobial solutions in a spray cabinet to inactivate natural microbial flora, nonpathogenic Escherichia coli , and Salmonella on jalapeno peppers. Jalapeno peppers, uninoculated or inoculated with a five-strain mixture of rifampin-resistant E. coli (3.9 log CFU/g) or novobiocin- and nalidixic acid-resistant Salmonella (4.2 log CFU/g), were passed through a commercial antimicrobial cabinet containing both a top and bottom bar spraying (1.38 bar and 2 liters/min) water, sodium hypochlorite (50 ppm), sodium hypochlorite with pH adjusted to 6...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28213029/antifungal-effect-of-volatile-organic-compounds-produced-by-bacillus-amyloliquefaciens-cpa-8-against-fruit-pathogen-decays-of-cherry
#2
A Gotor-Vila, N Teixidó, A Di Francesco, J Usall, L Ugolini, R Torres, M Mari
The present work focuses on the antifungal effect of volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens CPA-8 against Monilinia laxa, M. fructicola and Botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. VOCs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. For this purpose, CPA-8 was grown on different media and cultured for 24 and 48 h at 30 °C before assays. Data showed that mycelial growth inhibition was higher when CPA-8 was grown on Tryptone Soya Agar (TSA) while no differences were generally observed when CPA-8 was cultured for either, 24 and 48 h...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28209327/outcomes-of-total-shoulder-arthroplasty-in-patients-younger-than-65-years-a-systematic-review
#3
REVIEW
Troy A Roberson, Jared C Bentley, James T Griscom, Michael J Kissenberth, Stefan J Tolan, Richard J Hawkins, John M Tokish
BACKGROUND: Despite concerns about the longevity of total shoulder arthroplasty (TSA) in young patients, it remains an attractive option because of the absence of superior options. METHODS: A systematic review was performed using searches of PubMed, Embase, and Cochrane databases. English-language studies were identified with search terms "total shoulder arthroplasty" (title/abstract) or "shoulder replacement" (title/abstract) and "young" (title/abstract) or "under 65 years of age" (title/abstract)...
February 10, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28194674/biomonitoring-of-atmospheric-pollution-a-novel-approach-for-the-evaluation-of-natural-and-anthropogenic-contribution-to-atmospheric-aerosol-particles
#4
Rosa Caggiano, Giuseppe Calamita, Serena Sabia, Serena Trippetta
The investigation of the potential natural and anthropogenic contribution to atmospheric aerosol particles by using lichen-bag technique was performed in the Agri Valley (Basilicata region, southern Italy). This is an area of international concern since it houses one of the largest European on-shore reservoirs and the biggest oil/gas pre-treatment plant (i.e., Centro Olio Val d'Agri (COVA)) within an anthropized context. In particular, the concentrations of 17 trace elements (Al, Ca, Cd, Cr, Cu, Fe, K, Li, Mg, Mn, Na, Ni, P, Pb, S, Ti, and Zn) were measured in lichen bags exposed in 59 selected monitoring points over periods of 6 months (from October 2011 to April 2012) and 12 months (from October 2011 to October 2012)...
February 13, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28191472/suppression-of-excessive-histone-deacetylases-activity-in-diabetic-hearts-attenuates-myocardial-ischemia-reperfusion-injury-via-mitochondria-apoptosis-pathway
#5
Yang Wu, Yan Leng, Qingtao Meng, Rui Xue, Bo Zhao, Liying Zhan, Zhongyuan Xia
Background. Histone deacetylases (HDACs) play a pivotal role in signaling modification and gene transcriptional regulation that are essential for cardiovascular pathophysiology. Diabetic hearts with higher HDACs activity were more vulnerable to myocardial ischemia/reperfusion (MI/R) injury compared with nondiabetic hearts. We are curious about whether suppression of excessive HDACs activity in diabetic heart protects against MI/R injury. Methods. Diabetic rats were subjected to 45 min of ischemia, followed by 3 h of reperfusion...
2017: Journal of Diabetes Research
https://www.readbyqxmd.com/read/28190668/resilience-correlates-with-outcomes-after-total-shoulder-arthroplasty
#6
John M Tokish, Michael J Kissenberth, Stefan J Tolan, Tariq I Salim, Josh Tadlock, Thomas Kellam, Catherine D Long, Ashley Crawford, Keith T Lonergan, Richard J Hawkins, Ellen Shanley
BACKGROUND: Resilience, characterized by an ability to bounce back or recover from stress, is increasingly recognized as a psychometric property affecting many outcomes' domains including quality of life, suicide risk in active-duty military personnel, and recovery in cancer patients. This study examines the correlation between resilience, as measured by the Brief Resilience Scale (BRS), and traditional outcome scores including the American Shoulder and Elbow Surgeons (ASES), Single Assessment Numeric Evaluation (SANE), and Penn scores in patients undergoing total shoulder arthroplasty (TSA)...
February 10, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28188612/hepatitis-b-immunoglobulin-during-pregnancy-for-prevention-of-mother-to-child-transmission-of-hepatitis-b-virus
#7
REVIEW
Ahizechukwu C Eke, George U Eleje, Uzoamaka A Eke, Yun Xia, Jiao Liu
BACKGROUND: Hepatitis is a viral infection of the liver. It is mainly transmitted between people through contact with infected blood, frequently from mother to baby in-utero. Hepatitis B poses significant risk to the fetus and up to 85% of infants infected by their mothers at birth develop chronic hepatitis B virus (HBV) infection. Hepatitis B immunoglobulin (HBIG) is a purified solution of human immunoglobulin that could be administered to the mother, newborn, or both. HBIG offers protection against HBV infection when administered to pregnant women who test positive for hepatitis B envelope antigen (HBeAg) or hepatitis B surface antigen (HBsAg), or both...
February 11, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28181684/exercise-based-cardiac-rehabilitation-for-adults-with-atrial-fibrillation
#8
REVIEW
Signe S Risom, Ann-Dorthe Zwisler, Pernille P Johansen, Kirstine L Sibilitz, Jane Lindschou, Christian Gluud, Rod S Taylor, Jesper H Svendsen, Selina K Berg
BACKGROUND: Exercise-based cardiac rehabilitation may benefit adults with atrial fibrillation or those who had been treated for atrial fibrillation. Atrial fibrillation is caused by multiple micro re-entry circuits within the atrial tissue, which result in chaotic rapid activity in the atria. OBJECTIVES: To assess the benefits and harms of exercise-based rehabilitation programmes, alone or with another intervention, compared with no-exercise training controls in adults who currently have AF, or have been treated for AF...
February 9, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28177048/use-of-fluorescent-oligonucleotide-probes-for-differentiation-between-paracoccidioides-brasiliensis-and-paracoccidioides-lutzii-in-yeast-and-mycelial-phase
#9
Thales Domingos Arantes, Raquel Cordeiro Theodoro, Marcus de Melo Teixeira, Eduardo Bagagli
BACKGROUND: Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible to culture. OBJECTIVE: In this study, we aimed to standardise an in situ hybridisation technique for the differentiation between the pathogenic species Paracoccidioides brasiliensis and Paracoccidioides lutzii, by using species-specific DNA probes targeting the internal transcribed spacer-1 (ITS-1) of the rRNA gene...
February 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28174211/overlapping-and-divergent-actions-of-structurally-distinct-hdac-inhibitors-in-cardiac-fibroblasts
#10
Katherine B Schuetze, Matthew S Stratton, Weston W Blakeslee, Michael F Wempe, Florence F Wagner, Edward B Holson, Yin-Ming Kuo, Andrew J Andrews, Tonya M Gilbert, Jacob M Hooker, Timothy A McKinsey
Inhibitors of zinc-dependent histone deacetylases (HDACs) profoundly impact cellular function by altering gene expression via changes in nucleosomal histone tail acetylation. Historically, investigators have employed pan-HDAC inhibitors, such as the hydroxamate trichostatin A (TSA), which simultaneously targets members of each of the three zinc-dependent HDAC classes (classes I, II and IV). More recently, class- and isoform-selective HDAC inhibitors have been developed, providing invaluable chemical biology probes for dissecting the roles of distinct HDACs in the control of various physiological and pathophysiological processes...
February 7, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28170242/eu-iii-complex-with-do3a-amino-phosphonate-ligand-as-a-concentration-independent-ph-responsive-contrast-agent-for-magnetic-resonance-spectroscopy-mrs
#11
Tereza Krchová, Vít Herynek, Andrea Gálisová, Jan Blahut, Petr Hermann, Jan Kotek
A new DOTA-like ligand H5do3aNP with a 2-[amino(methylphosphonic acid)]ethyl-coordinating pendant arm was prepared, and its coordinating properties were studied by NMR spectroscopy and potentiometry. The study revealed a rare slow exchange (on the (1)H and (31)P NMR time scale) between protonated and unprotonated complex species with a corresponding acidity constant pKA ∼ 8.0. This unusually slow time scale associated with protonation is caused by a significant geometric change from square-antiprismatic (SA) arrangement observed for protonated complex SA-[Eu(Hdo3aNP)](-) to twisted-square-antiprismatic (TSA) arrangement found for deprotonated complex TSA-[Eu(do3aNP)](2-)...
February 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28164251/state-dependent-block-of-voltage-gated-sodium-channels-by-the-casein-kinase-1-inhibitor-ic261
#12
Karl J Föhr, Uwe Knippschild, Anna Herkommer, Michael Fauler, Christian Peifer, Michael Georgieff, Oliver Adolph
Background and Purpose IC261 (3-[(2,4,6-trimethoxyphenyl)methylidenyl]-indolin-2-one) has previously been introduced as an isoform specific inhibitor of casein kinase 1 (CK1) causing cell cycle arrest or cell death of established tumor cell lines. However, it is reasonable to assume that not all antitumor activities of IC261 are mediated by the inhibition of CK1. Meanwhile there is growing evidence that functional voltage-gated sodium channels are also implicated in the progression of tumors as their blockage suppresses tumor migration and invasion of different tumor cell lines...
February 6, 2017: Investigational New Drugs
https://www.readbyqxmd.com/read/28162056/tanshinone-%C3%A2-a-elicits-neuroprotective-effect-via-activating-the-nuclear-factor-erythroid-2-related-factor-dependent-antioxidant-response
#13
Min Cai, Yongxin Guo, Shiquan Wang, Haidong Wei, Guangchao Zhao, Sisi Sun, Hailong Dong
Tanshinone ⅡA (TSA), a principal component derived from the Traditional Chinese Medicine Danshen has been suggested to exert neuroprotective effect against experimental cerebral ischemic/reperfusion injury. But the associated underlying mechanisms still have not been understood. The current study characterized the role of nuclear factor erythroid 2-related factor induced antioxidant response in the neuroprotective efficacy of TSA treatment. The focal cerebral ischemia/reperfusion model was established by 60 minutes middle cerebral artery occlusion...
February 4, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28160167/histone-deacetylase-inhibitors-vpa-and-tsa-induce-apoptosis-and-autophagy-in-pancreatic-cancer-cells
#14
Maria Saveria Gilardini Montani, Marisa Granato, Claudio Santoni, Paola Del Porto, Nicolò Merendino, Gabriella D'Orazi, Alberto Faggioni, Mara Cirone
PURPOSE: Histone deacetylase inhibitors (HDACi) are anti-neoplastic agents that are known to affect the growth of different cancer types, but their underlying mechanisms are still incompletely understood. Here, we compared the effects of two HDACi, i.e., Trichostatin A (TSA) and Valproic Acid (VPA), on the induction of cell death and autophagy in pancreatic cancer-derived cells that exhibit a high metastatic capacity and carry KRAS/p53 double mutations. METHODS: Cell viability and proliferation tests were carried out using Trypan blue dye exclusion, MTT and BrdU assays...
February 3, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28159474/proximal-ingrowth-coating-decreases-risk-of-loosening-following-uncemented-shoulder-arthroplasty-using-mini-stem-humeral-components-and-lesser-tuberosity-osteotomy
#15
Michael P Morwood, Peter S Johnston, Grant E Garrigues
BACKGROUND: Mini-stem humeral component (MSHC) use during total shoulder arthroplasty (TSA) provides bone preservation and ease of revision. MSHCs rely solely on proximal metaphyseal fixation; some early reports have demonstrated an unacceptably high rate of early loosening. To our knowledge, no study analyzing the effect of proximal porous coating on MSHCs has been performed. METHODS: We performed a retrospective review of consecutive patients who underwent anatomic TSA using coated or uncoated MSHCs with minimum 2-year follow-up...
January 31, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28153684/analysis-of-complication-rates-following-perioperative-transfusion-in-shoulder-arthroplasty
#16
A Jordan Grier, Abiram Bala, Colin T Penrose, Thorsten M Seyler, Michael P Bolognesi, Grant E Garrigues
BACKGROUND: Postoperative anemia requiring a blood transfusion is not uncommon following anatomic total shoulder arthroplasty (TSA) or reverse total shoulder arthroplasty (RTSA). However, the potential complications in patients undergoing transfusion after shoulder arthroplasty remain unclear. The goal of this study was to examine the postoperative outcomes of patients receiving blood transfusions following TSA and RTSA. METHODS: Using the Medicare Standard Analytic Files database, we identified all patients undergoing TSA or RTSA between 2005 and 2010...
January 30, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28153571/providing-data-for-serrated-polyp-detection-rate-benchmarks-an-analysis-of-the-new-hampshire-colonoscopy-registry
#17
Joseph C Anderson, Lynn F Butterly, Julia E Weiss, Christina M Robinson
BACKGROUND AND AIMS: Similar to achieving adenoma detection rate (ADR) benchmarks to prevent colorectal cancer (CRC), achieving adequate serrated polyp detection rates (SDR) may be essential to the prevention of CRC associated with the serrated pathway. Previous studies have been based on data from high-volume endoscopists at single academic centers. Based on a hypothesis that ADR is correlated with SDR, we stratified a large, diverse group of endoscopists (n=77 practicing at 28 centers) into high and low performers based on ADR, to provide data for corresponding target SDR benchmarks...
January 30, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28148463/complications-in-total-shoulder-and-reverse-total-shoulder-arthroplasty-by-body-mass-index
#18
Oke Anakwenze, Alex Fokin, Mary Chocas, Mark T Dillon, Ronald A Navarro, Edward H Yian, Anshuman Singh
INTRODUCTION: The purpose of this study was to identify the effects of body mass index (BMI) on long-term outcomes (revision rate, 1-year mortality rate, 3-year surgical site infection rate, and 90-day inpatient all-cause readmission rate) after total shoulder arthroplasty (TSA) and reverse TSA (RTSA). METHODS: A large shoulder arthroplasty registry was used to review outcomes after TSA and RTSA. The registry monitors patient's revision, mortality, infection, and readmission rates...
January 30, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28144687/-trial-sequential-analysis-sample-size-calculation-for-reliable-meta-analyses
#19
S Weibel, P Kranke
BACKGROUND: Meta-analyses have a great impact on medical decision-making. Random errors are, however, often the reason for misinterpretation of interventional effects in meta-analyses. OBJECTIVE: The aim of this article is to introduce authors and readers of meta-analyses to the problem of random errors. The article presents trial sequential analysis (TSA) as a suitable and user-friendly method that adjusts for the risk of random errors in meta-analyses. MATERIAL AND METHODS: The practical application of TSA is illustrated and exemplified using regional anesthesiology procedures versus conventional pain therapy with respect to the prevention of persistent postoperative pain after breast cancer surgery or thoracotomy...
January 31, 2017: Der Anaesthesist
https://www.readbyqxmd.com/read/28139701/the-value-of-resting-state-functional-mri-in-subacute-ischemic-stroke-comparison-with-dynamic-susceptibility-contrast-enhanced-perfusion-mri
#20
Ling Ni, Jingwei Li, Weiping Li, Fei Zhou, Fangfang Wang, Christopher G Schwarz, Renyuan Liu, Hui Zhao, Wenbo Wu, Xin Zhang, Ming Li, Haiping Yu, Bin Zhu, Arno Villringer, Yufeng Zang, Bing Zhang, Yating Lv, Yun Xu
To evaluate the potential clinical value of the time-shift analysis (TSA) approach for resting-state fMRI (rs-fMRI) blood oxygenation level-dependent (BOLD) data in detecting hypoperfusion of subacute stroke patients through comparison with dynamic susceptibility contrast perfusion weighted imaging (DSC-PWI). Forty patients with subacute stroke (3-14 days after neurological symptom onset) underwent MRI examination. Cohort A: 31 patients had MRA, DSC-PWI and BOLD data. Cohort B: 9 patients had BOLD and MRA data...
January 31, 2017: Scientific Reports
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