Pei-Pei Yang, Yi-Jing Li, Yan Cao, Lu Zhang, Jia-Qi Wang, Ziwei Lai, Kuo Zhang, Diedra Shorty, Wenwu Xiao, Hui Cao, Lei Wang, Hao Wang, Ruiwu Liu, Kit S Lam
Self-assembling peptides have shown tremendous potential in the fields of material sciences, nanoscience, and medicine. Because of the vast combinatorial space of even short peptides, identification of self-assembling sequences remains a challenge. Herein, we develop an experimental method to rapidly screen a huge array of peptide sequences for self-assembling property, using the one-bead one-compound (OBOC) combinatorial library method. In this approach, peptides on beads are N-terminally capped with nitro-1,2,3-benzoxadiazole, a hydrophobicity-sensitive fluorescence molecule...
July 23, 2021: Nature Communications