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https://www.readbyqxmd.com/read/29337282/comparative-proteome-analysis-of-monolayer-and-spheroid-culture-of-canine-osteosarcoma-cells
#1
Christiane Gebhard, Ingrid Miller, Karin Hummel, Martina Neschi Née Ondrovics, Sarah Schlosser, Ingrid Walter
Osteosarcoma is an aggressive bone tumor with high metastasis rate in the lungs and affects both humans and dogs in a similar way. Three-dimensional tumor cell cultures mimic the in vivo situation of micro-tumors and metastases and are therefore better experimental in vitro models than the often applied two-dimensional monolayer cultures. The aim of the present study was to perform comparative proteomics of standard monolayer cultures of canine osteosarcoma cells (D17) and three-dimensional spheroid cultures, to better characterize the 3D model before starting with experiments like migration assays...
January 11, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29333015/a-profile-of-pediatric-solid-tumors-a-single-institution-experience-in-kashmir
#2
Namita Sharma, Ayesha Ahmad, Gull M Bhat, Sheikh A Aziz, Mohammad Maqbool Lone, Nisar A Bhat
Aims: The purpose of this retroprospective study was to study the epidemiological characteristics and outcomes of children with solid tumors at our institution. Subjects and Methods: Three hundred and three pediatrics patients registered at Regional Cancer Centre (RCC), Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Srinagar, Kashmir, between January 2008 and June 2014, were analyzed with regard to demographic status, presenting complaints, investigations, treatment, morbidity, and outcomes...
October 2017: Indian Journal of Medical and Paediatric Oncology
https://www.readbyqxmd.com/read/29313943/pediatric-oncologist-willingness-to-offer-germline-tp53-testing-in-osteosarcoma
#3
Eliana Shaul, Michael Roth, Yungtai Lo, David S Geller, Bang Hoang, Rui Yang, David Malkin, Richard Gorlick, Jonathan Gill
BACKGROUND: Li-Fraumeni syndrome (LFS) is a cancer predisposition syndrome caused by mutations in the tumor-suppressor gene TP53. Osteosarcoma is a sentinel cancer in LFS. Prior studies using Sanger sequencing platforms have demonstrated that 3% of individuals with osteosarcoma harbor a mutation in TP53. New data from next-generation sequencing have demonstrated that 3.8% of patients with osteosarcoma have a known pathogenic variant, and an additional 5.7% carry exonic variants of unknown significance in TP53...
January 3, 2018: Cancer
https://www.readbyqxmd.com/read/29291409/long-noncoding-rna-pcat-1-acts-as-an-oncogene-in-osteosarcoma-by-reducing-p21-levels
#4
Jiang Huang, Gaorong Deng, Tianmi Liu, Wenzhao Chen, Yang Zhou
Long non-coding RNA (lncRNA) is emerging as a critical regulator in multiple cancers. Recently, lncRNA PCAT-1 was found to be up-regulated in prostate cancer and hepatocellular carcinoma, exerting oncogenic effects. However, the biological function and regulatory mechanism of PCAT-1 remain unclear in osteosarcoma (OS). In this study, we reported that PCAT-1 expression was also upregulated in OS tissues, and its overexpression was remarkably associated with tumor size, Enneking stage, tumor node metastasis (TNM) stage and metastasis in patients with OS...
December 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29287201/mir-365-functions-as-a-tumor-suppressor-by-directly-targeting-cyr61-in-osteosarcoma
#5
Yawei Xu, Haijiao Chu, Yan Zhou, Junling Wang, Changying Dong, Rui Yin
Increasing evidence indicates that microRNAs(miRNAs) are often aberrantly expressed in osteosarcoma (OS) and play critical roles in OS tumorigenesis. Therefore, the discovery of miRNAs may provide a new and powerful tool for understanding the mechanismof OS initiation and development. The aim of this study was to investigate the functional significance of miR-365 and identify its possible mechanism in OS cells. Here, wefound that the expression level of miR-365 is significantly downregulated in OS tissues and cell lines, and its expression isassociated with the clinical stage, distant metastasis, tumor grade, and poor overall survival rate...
December 26, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29275200/long-non-coding-rna-ccal-promotes-hepatocellular-carcinoma-progression-by-regulating-ap-2%C3%AE-and-wnt-%C3%AE-catenin-pathway
#6
Yu Liu, Ye Yang, Tianlong Wang, Li Wang, Xin Wang, Tiemin Li, Yue Shi, Yawei Wang
OBJECTIVE: Long non-coding RNAs are emerging as key molecules in cancer progression. LncRNA-CCAL has shown to be highly expressed and important in regulating CRC and osteosarcoma development. Nevertheless, the expression and mechanism of CCAL in HCC is still not well understood. METHODS: qRT-PCR and ISH were used to evaluate CCAL expression in HCC tissues and cell lines. Histone H3 methylation and acetylation levels across CCAL promoter region were examined by chromatin immunoprecipitation assays...
December 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29251324/microrna-505-is-downregulated-in-human-osteosarcoma-and-regulates-cell-proliferation-migration-and-invasion
#7
Yu-Jiang Liu, Wei Li, Feng Chang, Jian-Na Liu, Jun-Xin Lin, De-Xi Chen
Recent studies have demonstrated that microRNAs (miRNAs/miRs) are involved in osteosarcoma tumorigenesis, progression, invasion and metastasis. For example, miR-505 plays important roles in human carcinogenesis; however, its exact function in osteosarcoma remains unclear. MicroRNA profiles of osteosarcoma and normal tissues were obtained by miRNA microarray assays, which were validated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Then, high-mobility group box 1 (HMGB1) expression was evaluated by qRT-PCR and western blot analysis...
February 2018: Oncology Reports
https://www.readbyqxmd.com/read/29250147/microrna-92b-promotes-cell-proliferation-and-invasion-in-osteosarcoma-by-directly-targeting-dickkopf-related-protein-3
#8
Qing Wu, Wei Zhou, Qiong Feng, Xing Liu, Yanfei Xiong, Hui Li
Deregulation of microRNA-92b (miR-92b) has been implicated in osteosarcoma. However, the underlying regulatory mechanism of miR-92b in osteosarcoma growth and metastasis remains largely unclear. In the present study, reverse transcription-quantitative polymerase chain reaction and western blotting were used to measure mRNA and protein expression. MTT and Transwell assays were conducted to determine cell proliferation and invasion, and a luciferase reporter assay was performed to confirm the association between miR-92b and Dickkopf3-related protein (DKK3)...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29246789/lncrna-crnde-promotes-osteosarcoma-cell-proliferation-invasion-and-migration-by-regulating-notch1-signaling-and-epithelial-mesenchymal-transition
#9
Zheng Li, Yonghua Tang, Wujun Xing, Wei Dong, Zhichou Wang
Osteosarcoma is the most common bone malignancy in adolescence. Recently, the long non-coding RNAs (lncRNAs) were reported to play important roles in osteosarcoma progression. The present study examined the potential role of the lncRNA, Colorectal Neoplasia Differentially Expressed (CRNDE) and molecular mechanisms underlying osteosarcoma progression. In the present study, we identified that CRNDE was up-regulated in osteosarcoma tissues and cell lines, and CRNDE expression level was significantly higher in osteosarcoma tissues from patients with advanced stage and metastasis...
December 12, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29243774/clinical-significance-of-long-non-coding-rna-ewsat1-as-a-novel-prognostic-biomarker-in-osteosarcoma
#10
G-Y Zhang, J-F Zhang, X-M Hu, Z-P Luo, Y-Z Ma
OBJECTIVE: Long non-coding RNA EWSAT1 (EWSAT1) has been identified as a tumor promoter in several tumors, but its prognostic values in osteosarcoma have not been reported. The purpose of this study was to explore the association between EWSAT1 expression and prognosis of osteosarcoma patients. PATIENTS AND METHODS: EWSAT1 levels were examined in 176 osteosarcoma tissues and matched normal bone tissues by qRT-PCR analysis. The associations of EWSAT1 expression with clinicopathologic variables were analyzed...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29232802/-sarcomatoid-urothelial-carcinoma-of-the-bladder-including-an-osteosarcoma-element
#11
Kouzaburou Tanuma, Koji Kawai, Haruki Tsuchiya, Yoshitaka Matsumoto, Shuya Kandori, Takahiro Kojima, Tomokazu Kimura, Akira Joraku, Jun Miyazaki, Hiroyuki Nishiyama, Akiko Sakata
A 68-year-old Japanese man was referred to Tsukuba University Hospital for bladder cancer treatment. He had undergone a transurethral resection of a bladder tumor (TURBT) at a local hospital, but the pathological specimen did not contain muscle layer. Abdominal computed tomography (CT) and magnetic resonance imaging revealed a 3 cm non-papillary bladder tumor with muscle invasion, but there was no apparent calcification. The patient underwent re-TURBT at our hospital for diagnosis and staging. A non-papillary pedunculated tumor was identified in the bladder dome, and it contained a small papillary part...
November 2017: Hinyokika Kiyo. Acta Urologica Japonica
https://www.readbyqxmd.com/read/29232358/genetic-polymorphisms-of-insulin-like-growth-factor-1-are-associated-with-osteosarcoma-risk-and-prognosis
#12
Jianshui Mao, Genying Zhuang, Zhikang Chen
BACKGROUND Insulin-like growth factor 1 (IGF-1) gene plays an important role in bone and soft tumors. IGF-1 gene polymorphisms have been revealed to be correlated with the carcinogenesis and progression of solid malignancies. We therefore hypothesized that IGF-1 genetic polymorphisms might be associated with the risks and outcomes of osteosarcomas in Chinese individuals. MATERIAL AND METHODS This study included 173 conventional osteosarcoma individuals and 175 tumor-free controls. Five single nucleotide polymorphisms (SNPs) of IGF-1 (rs6214, rs6218, rs35767, rs5742612, and rs5742714) were genotyped...
December 12, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29228583/development-and-validation-of-nomogram-based-on-mir-203-and-clinicopathological-characteristics-predicting-survival-after-neoadjuvant-chemotherapy-and-surgery-for-patients-with-non-metastatic-osteosarcoma
#13
Dong Cheng, Xubin Qiu, Ming Zhuang, Chenlei Zhu, Hongjun Zou, Ailiang Zhang
Background: Recently, nomograms have been used as models for risk prediction in malignant tumor because they can predict the outcome of interest for a certain individual based on many variables. This study aimed to establish an effective prognostic nomogram for osteosarcoma based on the clinicopathological factors and microRNA-203. Results: The results showed that miR-203 expression was significantly lower in osteosarcoma tissues compared with the corresponding adjacent tissues (P < 0...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29226461/long-non-coding-pandar-as-a-novel-biomarker-in-human-cancer-a-systematic-review
#14
REVIEW
Yifan Zou, Yuantang Zhong, Junjie Wu, Huizhong Xiao, Xintao Zhang, Xinhui Liao, Jianfa Li, Xuhua Mao, Yuchen Liu, Fuyou Zhang
OBJECTIVES: Long non-coding RNAs (lncRNAs) are characterized as a group of RNAs that more than 200 nucleotides in length and have no protein-coding function. More and more evidences provided that lncRNAs serve as key molecules in the development of cancer. Deregulation of lncRNAs functions as either oncogenes or tumour suppressor genes in various diseases. Recently, increasing studies about PANDAR in cancer progression were reported. In our review, we will focus on the current research on the character of PANDAR include the clinical management, tumour progression and molecular mechanisms in human cancers...
December 10, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29207112/microrna-381-suppresses-the-proliferation-of-osteosarcoma-cells-through-lrh-1-wnt-%C3%AE-catenin-signaling-pathway
#15
Yanbing Wang, Shicai Sun, Jianting Xu, Yang Luo, Ying Xin, Xin Zhao
We explored the function of microRNA-381 on osteosarcoma cell growth and anticancer mechanism for clinic treatment. MicroRNA-381 of osteosarcoma patients was decreased, microRNA-381 levels were more downregulated at stages III/IV than those at stage I/II in osteosarcoma patients. Downregulation of microRNA-381 using anti-microRNA-381 mimics increased cell proliferation, decreased LDH activity and apoptosis, and inhibited caspase‑3/9 activities, Bax/Bcl-2 and p53 protein expression in vitro osteosarcoma cells through upregulation of LRH-1/Wnt/β-catenin signaling pathway...
December 4, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29203385/the-effect-of-dna-pkcs-gene-silencing-on-proliferation-migration-invasion-and-apoptosis-and-in-vivo-tumorigenicity-of-human-osteosarcoma-mg-63-cells
#16
Pei-Ying Jin, Hong-Jie Lu, Yao Tang, Shao-Hua Fan, Zi-Feng Zhang, Yan Wang, Xu-Ning Li, Dong-Mei Wu, Jun Lu, Yuan-Lin Zheng
The purpose of this study was to explore the role by which the DNA-dependent protein kinase complex catalytic subunit (DNA-PKcs) influences osteosarcoma MG-63 cell apoptosis, proliferation, migration and invasion. Osteosarcoma tissues and adjacent normal tissues were obtained from 57 osteosarcoma patients. Human osteosarcoma MG-63 cells were assigned into designated groups including the blank, siRNA-negative control (NC) and siRNA-DNA-PKcs groups. RT-qPCR and Western blotting methods were employed to evaluate the mRNA and protein expressions of DNA-PKcs...
December 1, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29200101/modeling-continuous-prognostic-factors-in-survival-analysis-implications-for-tumor-staging-and-assessing-chemotherapy-effect-in-osteosarcoma
#17
Justin M M Cates
Extent of response to neoadjuvant chemotherapy, tumor size, and patient age are important prognostic variables for patients with osteosarcoma, but applying information from these continuous variables in survival models is difficult. Dichotomization is usually inappropriate and alternative statistical techniques should be considered instead. Nonlinear multivariable regression methods (restricted cubic splines and fractional polynomials) were applied to data from the National Cancer Database to model continuous prognostic factors for overall survival from localized, high-grade osteosarcoma of the appendicular and nonspinal skeleton following neoadjuvant chemotherapy and surgical resection (N=2493)...
December 1, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29194827/downregulation-of-lncrna-casc2-facilitates-osteosarcoma-growth-and-invasion-through-mir-181a
#18
Zhiwen Ba, Lili Gu, Songnan Hao, Xiaofang Wang, Zhenping Cheng, Guangchen Nie
OBJECTIVES: Long non-coding RNA cancer susceptibility candidate 2 (CASC2) is a novel lncRNA and has been indicated as playing tumour suppressor gene in several tumours. However, the role of CASC2 in osteosarcoma is still uncovered. MATERIALS AND METHODS: The CASC2 and miR-181a expressions were measured via qRT-PCR. CCK-8 assay and colony formation assay were performed to determine the cell growth, and transwell assay was performed to assess the cell invasion. RESULTS: We showed that CASC2 expression was downregulated in osteosarcoma samples and cell lines...
November 30, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29182106/multidisciplinary-approach-to-osteosarcoma
#19
A Biazzo, M De Paolis
Osteosarcoma is a malignant bone tumor composed of mesenchymal cells producing osteoid and immature bone. Osteosarcoma is the most frequent primary malignant bone tumor, if we excluded myeloma, a haematologic disease. The incidence of osteosarcoma is 2-3/million/year, but is higher in adolescence, in which the annual incidence peaks at 8-11/million/year at 15-19 years of age. Local pain, followed by localized swelling and limitation of joint movement, are the typical signs and symptoms. Correct diagnosis can be achieved through a correct approach to the disease and the combination of clinical and radiographic aspects...
December 2016: Acta Orthopaedica Belgica
https://www.readbyqxmd.com/read/29181065/down-regulation-of-microrna-182-and-microrna-183-predicts-progression-of-osteosarcoma
#20
Mohammad Reza Golbakhsh, Bahram Boddouhi, Negin Hatami, Peyman Karimi Goudarzi, Mohammadreza Shakeri, Emad Yahaghi, Afshin Taheriazam
Introduction: The aim of this study was to investigate the expression levels of microRNA-182 and microRNA-183 and their association with clinicopathological features in patients with osteosarcoma. Material and methods: Total RNA was purified from samples and noncancerous bone tissues and then quantitative real-time polymerase chain reaction was applied to evaluate the expression levels of microRNAs, and their relationship with clinicopathological features and survival in osteosarcoma patients...
October 2017: Archives of Medical Science: AMS
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