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https://www.readbyqxmd.com/read/28547818/self-expansion-construction-of-ultralight-carbon-nanotube-aerogels-with-a-3d-and-hierarchical-cellular-structure
#1
Yufeng Luo, Shu Luo, Hengcai Wu, Mengya Li, Ke Wang, Lingjia Yan, Kaili Jiang, Qunqing Li, Shoushan Fan, Jiaping Wang
A novel and simple strategy is developed to construct ultralight and 3D pure carbon nanotube (CNT) aerogels by the spontaneous expansion of superaligned CNT films soaked in a piranha (mixed H2 SO4 and H2 O2 ) solution, followed by cryodesiccation. The macroscopic CNT aerogels have an extremely low apparent density (0.12 mg cm(-3) ), ultrahigh porosity (99.95%), high specific surface area (298 m(2) g(-1) ), and a hierarchical cellular structure with giant and ultrathin CNT sheets as cell walls. The pure CNT aerogels show high adsorption abilities for various kinds of solvents, and have great potential in widespread applications such as energy storage, catalysis, and bioengineering...
May 26, 2017: Small
https://www.readbyqxmd.com/read/28546353/meeting-report-for-the-2017-national-institutes-of-health-national-heart-lung-and-blood-institute-progenitor-cell-biology-consortium-cardiovascular-bioengineering-workshop-and-symposium
#2
https://www.readbyqxmd.com/read/28544641/reflectance-spectroscopy-to-shed-new-light-on-the-capillary-refill-test
#3
Rani Toll John, Joakim Henricson, Gert E Nilsson, Daniel Wilhelms, Chris D Anderson
To use Bioengineering methodology is used to achieve, at five anatomical sites, a detailed, quantitative assessment of the return of blood content to the blanched area, during the Capillary Refill (CR) test. An observational, non-randomized, experimental study on 23 healthy subjects (14 females) was performed in our climate controlled skin physiology laboratory. Our main outcome measures were based on the chronological assessment and quantification of red blood cell concentration (RBC) after the release of blanching pressure in the CR test, using Tissue Viability Imaging (TiVi), a digital photographic technique based on polarisation spectroscopy...
May 24, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28542773/biotechnology-and-bioengineering-volume-114-number-7-july-2017
#4
(no author information available yet)
No abstract text is available yet for this article.
July 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28541773/induction-of-salivary-gland-like-cells-from-dental-follicle-epithelial-cells
#5
Q L Xu, A Furuhashi, Q Z Zhang, C M Jiang, T-H Chang, A D Le
The dental follicle (DF), most often associated with unerupted teeth, is a condensation of ectomesenchymal cells that surrounds the tooth germ in early stages of tooth development. In the present study, we aim to isolate epithelial stem-like cells from the human DF and explore their potential differentiation into salivary gland (SG) cells. We demonstrated the expression of stem cell-related genes in the epithelial components of human DF tissues, and these epithelial progenitor cells could be isolated and ex vivo expanded in a reproducible manner...
May 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28536347/the-current-use-of-stem-cells-in-bladder-tissue-regeneration-and-bioengineering
#6
REVIEW
Yvonne Y Chan, Samantha K Sandlin, Eric A Kurzrock, Stephanie L Osborn
Many pathological processes including neurogenic bladder and malignancy necessitate bladder reconstruction, which is currently performed using intestinal tissue. The use of intestinal tissue, however, subjects patients to metabolic abnormalities, bladder stones, and other long-term sequelae, raising the need for a source of safe and reliable bladder tissue. Advancements in stem cell biology have catapulted stem cells to the center of many current tissue regeneration and bioengineering strategies. This review presents the recent advancements in the use of stem cells in bladder tissue bioengineering...
January 6, 2017: Biomedicines
https://www.readbyqxmd.com/read/28535986/intracellular-cellobiose-metabolism-and-its-applications-in-lignocellulose-based-biorefineries
#7
REVIEW
Vinuselvi Parisutham, Sathesh-Prabu Chandran, Aindrila Mukhopadhyay, Sung Kuk Lee, Jay D Keasling
Complete hydrolysis of cellulose has been a key characteristic of biomass technology because of the limitation of industrial production hosts to use cellodextrin, the partial hydrolysis product of cellulose. Cellobiose, a β-1,4-linked glucose dimer, is a major cellodextrin of the enzymatic hydrolysis (via endoglucanase and exoglucanase) of cellulose. Conversion of cellobiose to glucose is executed by β-glucosidase. The complete extracellular hydrolysis of celluloses has several critical barriers in biomass technology...
May 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28534765/the-animal-kingdom-is-also-a-bioengineering-field-exploring-the-art-and-science-of-vetinary-medicine-retrospectroscope
#8
Max E Valentinuzzi
Medical science developed in tandem with the evolution of biological species and their associated diseases. Because of the close interaction between humans and other animals, even those in the wild, taking care of the former also means caring for the latter. Several scientific forerunners delved into animals' anatomical and physiological secrets in their quest to better understand animal biology and functions, thereby laying the foundation for animal medicine. Here, I briefly explore the long and complex road that led to the current state of veterinary science and provide a few examples of its present standing...
May 2017: IEEE Pulse
https://www.readbyqxmd.com/read/28534757/tissue-engineering-and-regenerative-medicine-the-promise-the-challenges-the-future
#9
Leslie Mertz
Medical researchers increasingly regard tissue engineering and regenerative medicine as potential game changers when it comes to repairing damage from disease or injury and restoring function. To understand the progress made and challenges ahead for this combined field, IEEE Pulse sought out two experts: David F. Williams, former global president (2012-2015) of the Tissue Engineering and Regenerative Medicine International Society, and Roderic I. Pettigrew, founding director of the National Institute of Biomedical Imaging and Bioengineering (NIBIB) at the National Institutes of Health (NIH) [1]...
May 2017: IEEE Pulse
https://www.readbyqxmd.com/read/28533324/crispr-cas9-genetic-modification-of-cyp3a5-3-in-huh-7-human-hepatocyte-cell-line-leads-to-cell-lines-with-increased-midazolam-and-tacrolimus-metabolism
#10
Casey R Dorr, Rory P Remmel, Amutha Muthusamy, James Fisher, Branden Moriarity, Kazuto Yasuda, Baolin Wu, Weihua Guan, Erin G Schuetz, William S Oetting, Pamala A Jacobson, Ajay K Israni
CRISPR/Cas9 engineering of the CYP3A5 *3 locus (rs776746) in human liver cell line HuH-7 (CYP3A5 *3/*3) led to three CYP3A5 *1 cell lines by deletion of the exon 3B splice junction or point mutation. Cell lines CYP3A5 *1/*3 sd (single deletion), CYP3A5 *1/*1 dd (double deletion) or CYP3A5 *1/*3 pm (point mutation) expressed the CYP3A5 *1 mRNA, had elevated CYP3A5 mRNA (p<0.0005 for all engineered cell lines) and protein expression compared with HuH-7. In metabolism assays, HuH-7 had less tacrolimus (Tac) (all p-values < 0...
May 22, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28531156/molecular-techniques-for-the-detection-of-organisms-in-aquatic-environments-with-emphasis-on-harmful-algal-bloom-species
#11
REVIEW
Linda K Medlin, Jahir Orozco
Molecular techniques to detect organisms in aquatic ecosystems are being gradually considered as an attractive alternative to standard laboratory methods. They offer faster and more accurate means of detecting and monitoring species, with respect to their traditional homologues based on culture and microscopic counting. Molecular techniques are particularly attractive when multiple species need to be detected and/or are in very low abundance. This paper reviews molecular techniques based on whole cells, such as microscope-based enumeration and Fluorescence In-Situ Hybridization (FISH) and molecular cell-free formats, such as sandwich hybridization assay (SHA), biosensors, microarrays, quantitative polymerase chain reaction (qPCR) and real time PCR (RT-PCR)...
May 22, 2017: Sensors
https://www.readbyqxmd.com/read/28530145/bioengineered-liposome-scaffold-composites-as-therapeutic-delivery-systems
#12
Claudia Zylberberg, Sandro Matosevic
The therapeutic potential of liposomes can be amplified when combined with biomaterial scaffolds. Such configurations overcome the convergent demands of therapies by enabling enhanced delivery, environmental responsiveness and potency. Liposomes benefit from the increased physical and mechanical strength, favorable rheological properties and natural environment conducive to improved tissue formation that scaffolds provide, while enabling biocompatible delivery of hydrophilic and lipophilic compounds that can be further functionalized to achieve targeted delivery...
June 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/28528855/patterns-of-recent-national-institutes-of-health-nih-funding-to-diagnostic-radiology-departments-analysis-using-the-nih-reporter-system
#13
Ana M Franceschi, Andrew B Rosenkrantz
RATIONALE AND OBJECTIVES: This study aimed to characterize recent National Institutes of Health (NIH) funding for diagnostic radiology departments at US medical schools. MATERIALS AND METHODS: This retrospective study did not use private identifiable information and thus did not constitute human subjects research. The public NIH Research Portfolio Online Reporting Tools Expenditure and Results system was used to extract information regarding 887 NIH awards in 2015 to departments of "Radiation-Diagnostic/Oncology...
May 18, 2017: Academic Radiology
https://www.readbyqxmd.com/read/28527695/cytoprotection-proliferation-and-epidermal-differentiation-of-adipose-tissue-derived-stem-cells-on-emu-oil-based-electrospun-nanofibrous-mat
#14
Younes Pilehvar-Soltanahmadi, Mohammad Nouri, Mikaël M Martino, Amir Fattahi, Effat Alizadeh, Masoud Darabi, Mohammad Rahmati-Yamchi, Nosratollah Zarghami
Electrospun nanofibrous scaffolds containing natural substances with wound healing properties such as Emu oil (EO) may have a great potential for increasing the efficiency of stem cell-based skin bioengineering. For this purpose, EO blended PCL/PEG electrospun nanofibrous mats were successfully fabricated and characterized using FE-SEM, FTIR and Universal Testing Machine. The efficiency of the scaffolds in supporting the adherence, cytoprotection, proliferation and differentiation of adipose tissue-derived stem cells (ADSCs) to keratinocyte was evaluated...
May 17, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28522212/bioengineering-bacterial-outer-membrane-vesicles-as-vaccine-platform
#15
REVIEW
Matthias J H Gerritzen, Dirk E Martens, René H Wijffels, Leo van der Pol, Michiel Stork
Outer membrane vesicles (OMVs) are naturally non-replicating, highly immunogenic spherical nanoparticles derived from Gram-negative bacteria. OMVs from pathogenic bacteria have been successfully used as vaccines against bacterial meningitis and sepsis among others and the composition of the vesicles can easily be engineered. OMVs can be used as a vaccine platform by engineering heterologous antigens to the vesicles. The major advantages of adding heterologous proteins to the OMV are that the antigens retain their native conformation, the ability of targeting specific immune responses, and a single production process suffices for many vaccines...
May 15, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28520913/when-theatre-comes-to-engineering-design-oh-how-creative-they-can-be
#16
Ferris M Pfeiffer, Rachel Bauer, Steve Borgelt, Suzanne Burgoyne, Sheila Grant, Heather Hunt, Jennie Pardoe, David Schmidt
The creative process is fun, complex, and sometimes frustrating, but it is critical to the future of our nation and progress in science, technology, engineering, mathematics, as well as other fields. Thus, we set out to see if implementing methods of active learning typical to the theatre department could impact the creativity of senior capstone design students in the Bioengineering department. Senior Bioengineering capstone design students were allowed to self-select into groups. Prior to the beginning of coursework, all students completed a validated survey measuring engineering design self-efficacy...
May 18, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28513457/bacteriocins-from-lactic-acid-bacteria-as-an-alternative-to-antibiotics
#17
Aleksandra Ołdak, Dorota Zielińska
Bacteriocins are ribosomally synthesized, proteinaceous substances that inhibit the growth of closely related species through numerous mechanisms. The classification system used in this review divided bacteriocins into four sub-groups based on their size. Currently, there is extensive research focused on bacteriocins and their usage as a food preservative. The increasing incidence of multidrug resistant bacterial pathogens is one of the most pressing medical problems in recent years. Recently, the potential clinical application of LAB (Lactic Acid Bacteria) bacteriocin has been the subject of investigations by many scientists...
May 5, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28512053/effect-of-chitosan-and-coagulation-factors-on-the-wound-repair-phenotype-of-bioengineered-blood-clots
#18
Caroline D Hoemann, Catherine Marchand, Georges-Etienne Rivard, Hani El-Gabalawy, Patrice E Poubelle
Controlling the blood clot phenotype in a surgically prepared wound is an evolving concept in scaffold-guided tissue engineering. Here, we investigated the effect of added chitosan (80% or 95% Degree of Deacetylation, DDA) or coagulation factors (recombinant human Factor VIIa, Tissue Factor, thrombin) on inflammatory factors released by blood clots. We tested the hypothesis that 80% DDA chitosan specifically enhances leukotriene B4 (LTB4) production. Human or rabbit whole blood was combined with isotonic chitosan solutions, coagulation factors, or lipopolysaccharide, cultured in vitro at 37°C, and after 4hours the serum was assayed for LTB4 or inflammatory factors...
May 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28507934/the-challenges-of-recombinant-endostatin-in-clinical-application-focus-on-the-different-expression-systems-and-molecular-bioengineering
#19
REVIEW
Abbas Mohajeri, Sarvin Sanaei, Farhad Kiafar, Amir Fattahi, Majid Khalili, Nosratollah Zarghami
Angiogenesis plays an essential role in rapid growing and metastasis of the tumors. Inhibition of angiogenesis is a putative strategy for cancer therapy. Endostatin (Es) is an attractive anti-angiogenesis protein with some clinical application challenges including; short half-life, instability in serum and requirement to high dosage. Therefore, production of recombinant endostatin (rEs) is necessary in large scale. The production of rEs is difficult because of its structural properties and is high-cost. Therefore, this review focused on the different expression systems that involved in rEs production including; mammalian, baculovirus, yeast, and Escherichia coli (E...
April 2017: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28505124/structure-activity-relationships-of-bioengineered-heparin-heparan-sulfates-produced-in-different-bioreactors
#20
Ha Na Kim, John M Whitelock, Megan S Lord
Heparin and heparan sulfate are structurally-related carbohydrates with therapeutic applications in anticoagulation, drug delivery, and regenerative medicine. This study explored the effect of different bioreactor conditions on the production of heparin/heparan sulfate chains via the recombinant expression of serglycin in mammalian cells. Tissue culture flasks and continuously-stirred tank reactors promoted the production of serglycin decorated with heparin/heparan sulfate, as well as chondroitin sulfate, while the serglycin secreted by cells in the tissue culture flasks produced more highly-sulfated heparin/heparan sulfate chains...
May 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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