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https://www.readbyqxmd.com/read/29235541/biodegradable-bisvinyl-sulfonemethyl-crosslinked-gelatin-conduit-promotes-regeneration-after-peripheral-nerve-injury-in-adult-rats
#1
Chien-Hsin Ko, Ming-You Shie, Jia-Horng Lin, Yi-Wen Chen, Chun-Hsu Yao, Yueh-Sheng Chen
In our previous study, we found that gelatin-based materials exhibit good conductivity and are non-cytotoxic. In this study, gelatin was cross-linked with bisvinyl sulfonemethyl (BVSM) to fabricate a biodegradable conduit for peripheral nerve repair. First, BVSM on the prepared conduit was characterized to determine its mechanical properties and contact angle. The maximum tensile strength and water contact angle of the gelatin-BVSM conduits were 23 ± 4.8 MPa and 74.7 ± 9°, which provided sufficient mechanical strength to resist muscular contraction; additionally, the surface was hydrophilic...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232436/cellular-versus-acellular-grafts-for-diabetic-foot-ulcers-altering-the-protocol-to-improve-recruitment-to-a-comparative-efficacy-trial
#2
Catherine N Tchanque-Fossuo, Sara E Dahle, Hadar Lev-Tov, Chin-Shang Li, R Rivkah Isseroff
Diabetic foot ulcers (DFUs) have a substantial impact on public health. The standard of care (SOC) for DFUs consists of a multidisciplinary approach involving glycemic control, wound care with debridement of necrotic tissue, application of a moist dressing, infection control, use of off-loading devices, and patient education. New therapeutic devices aim to target the extracellular matrix (ECM) that is impaired in DFU; however, there is insufficient data on the effectiveness of such therapies along with lack of evidence on their long-term effectiveness...
November 2017: Cutis; Cutaneous Medicine for the Practitioner
https://www.readbyqxmd.com/read/29231863/silk-materials-functionalized-via-genetic-engineering-for-biomedical-applications
#3
REVIEW
Tomasz Deptuch, Hanna Dams-Kozlowska
The great mechanical properties, biocompatibility and biodegradability of silk-based materials make them applicable to the biomedical field. Genetic engineering enables the construction of synthetic equivalents of natural silks. Knowledge about the relationship between the structure and function of silk proteins enables the design of bioengineered silks that can serve as the foundation of new biomaterials. Furthermore, in order to better address the needs of modern biomedicine, genetic engineering can be used to obtain silk-based materials with new functionalities...
December 12, 2017: Materials
https://www.readbyqxmd.com/read/29229957/plants-increase-co2-uptake-by-assimilating-nitrogen-via-the-photorespiratory-pathway
#4
Florian A Busch, Rowan F Sage, Graham D Farquhar
Photorespiration is a major bioengineering target for increasing crop yields as it is often considered a wasteful process. Photorespiratory metabolism is integrated into leaf metabolism and thus may have certain benefits. Here, we show that plants can increase their rate of photosynthetic CO2 uptake when assimilating nitrogen de novo via the photorespiratory pathway by fixing carbon as amino acids in addition to carbohydrates. Plants fed NO3- had higher rates of CO2 assimilation under photorespiratory than low-photorespiratory conditions, while plants lacking NO3- nutrition exhibited lower stimulation of CO2 uptake...
December 11, 2017: Nature Plants
https://www.readbyqxmd.com/read/29229544/a-new-textured-polyphosphazene-biomaterial-with-improved-blood-coagulation-and-microbial-infection-responses
#5
Li-Chong Xu, Zhongjing Li, Zhicheng Tian, Chen Chen, Harry R Allcock, Christopher A Siedlecki
A new poly[bis(octafluoropentoxy) phosphazene] (OFP) was synthesized for the purpose of blood contacting medical devices. OFP was further either developed into crosslinkable polyphosphazene (X-OFP) or blended with polyurethane (PU) as the mixture (OFP/PU) for improvement of mechanical property of polyphosphazene polymers. All the materials were fabricated as smooth films or further textured with submicron pillars for the assay of antimicrobial and antithrombotic properties. Results showed that crosslinkable OFP (X-OFP) and blends of OFP/PU successfully improved the mechanical strength of OFP and fewer defects of pillars were found on the textured polyphosphazene surfaces...
December 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29229120/susceptibility-of-eptfe-vascular-grafts-and-bioengineered-human-acellular-vessels-to-infection
#6
Robert D Kirkton, Heather L Prichard, Maribel Santiago-Maysonet, Laura E Niklason, Jeffrey H Lawson, Shannon L M Dahl
BACKGROUND: Synthetic expanded polytetrafluorethylene (ePTFE) grafts are routinely used for vascular repair and reconstruction but prone to sustained bacterial infections. Investigational bioengineered human acellular vessels (HAVs) have shown clinical success and may confer lower susceptibility to infection. Here we directly compared the susceptibility of ePTFE grafts and HAV to bacterial contamination in a preclinical model of infection. MATERIALS AND METHODS: Sections (1 cm2) of ePTFE (n = 42) or HAV (n = 42) were inserted within bilateral subcutaneous pockets on the dorsum of rats and inoculated with Staphylococcus aureus (107 CFU/0...
January 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29227626/cellular-bioparticulates-with-therapeutics-for-cancer-immunotherapy
#7
Chao Wang, Di Wen, Zhen Gu
Cancer immunotherapy has received considerable attention because of a variety of exciting clinical outcomes in recent years. At the interface of cancer immunotherapy and bioengineering, there are many opportunities to further improve the treatment efficacy or address current challenges in immunotherapy. Cellular bioparticulates as promising carriers of immunomodulators have attracted much attention due to their inherent biocompatibility and unique biological properties. This Topical Review highlights recently reported studies utilizing bioparticulates for cancer immunotherapy, including engineered red blood cells (RBC) and RBC membrane coated particles, engineered T-cells, engineered platelets, and engineered microbes...
December 11, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29226477/cell-therapy-for-critical-limb-ischemia-an-integrated-review-of-pre-clinical-and-clinical-studies
#8
REVIEW
Mohammad Qadura, Daniella C Terenzi, Subodh Verma, Mohammed Al-Omran, David A Hess
Critical limb ischemia (CLI), the most severe form of peripheral artery disease, is characterized by pain at rest and non-healing ulcers in the lower extremities. For patients with CLI, where the extent of atherosclerotic artery occlusion is too severe for surgical bypass or percutaneous interventions, limb amputation remains the only treatment option. Thus, cell-based therapy to restore perfusion and promote wound healing in patients with CLI is under intense investigation. Despite promising preclinical studies in animal models, transplantation of bone marrow (BM) derived cell populations in patients with CLI has shown limited benefit preventing limb amputation...
December 11, 2017: Stem Cells
https://www.readbyqxmd.com/read/29226358/mechanical-evaluation-of-tracheal-grafts-on-different-scales
#9
REVIEW
Fabio Gava Aoki, Henrique Takachi Moriya
Tissue engineered (or bioengineered) tracheas are alternative options under investigation when the resection with end-to-end anastomosis cannot be performed. One approach to develop bioengineered tracheas is a complex process that involves the use of decellularized tissue scaffolds, followed by recellularization in custom-made tracheal bioreactors. Tracheas withstand pressure variations and their biomechanics are of great importance so that they do not collapse during respiration, although there has been no preferred method of mechanical assay of tracheas among several laboratories over the years...
December 11, 2017: Artificial Organs
https://www.readbyqxmd.com/read/29222482/poly-methyl-vinyl-ether-alt-maleic-acid-and-ethyl-monoester-as-building-polymers-for-drug-loadable-electrospun-nanofibers
#10
Amalia Mira, C Reyes Mateo, Ricardo Mallavia, Alberto Falco
New biomaterials are sought for the development of bioengineered nanostructures. In the present study, electrospun nanofibers have been synthesized by using poly(methyl vinyl ether-alt-maleic acid) and poly(methyl vinyl ether-alt-maleic ethyl monoester) (PMVEMA-Ac and PMVEMA-ES, respectively) as building polymers for the first time. To further functionalize these materials, nanofibers of PMVEMA-Ac and PMVEMA-ES containing a conjugated polyelectrolyte (HTMA-PFP, blue emitter, and HTMA-PFNT, red emitter) were achieved with both forms maintaining a high solid state fluorescence yield without altered morphology...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29218360/bioengineered-cardiac-tissue-based-on-human-stem-cells-for-clinical-application
#11
Monica Jara Avaca, Ina Gruh
Engineered cardiac tissue might enable novel therapeutic strategies for the human heart in a number of acquired and congenital diseases. With recent advances in stem cell technologies, namely the availability of pluripotent stem cells, the generation of potentially autologous tissue grafts has become a realistic option. Nevertheless, a number of limitations still have to be addressed before clinical application of engineered cardiac tissue based on human stem cells can be realized. We summarize current progress and pending challenges regarding the optimal cell source, cardiomyogenic lineage specification, purification, safety of genetic cell engineering, and genomic stability...
December 8, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/29216777/the-long-and-winding-road-stem-cells-for-cystic-fibrosis
#12
Massimo Conese, Elisa Beccia, Stefano Castellani, Sante Di Gioia, Carla Colombo, Antonella Angiolillo, Annalucia Carbone
Cystic fibrosis (CF) is a genetic syndrome with a high mortality rate due to severe lung disease. Despite having several drugs targeting specific mutated CFTR proteins already in clinical trials, new therapies, based on stem cells, are also emerging to treat those patients. Areas covered: The authors review the main sources of stem cells, including embryonic stem cells (ESCs), induced-pluripotent stem cells (iPSCs), gestational stem cells, and adult stem cells, such as mesenchymal stem cells (MSCs) in the context of CF...
December 8, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/29215714/photobiomodulation-of-mesenchymal-stem-cells-encapsulated-in-an-injectable-rhbmp4-loaded-hydrogel-directs-hard-tissue-bioengineering
#13
Ivana Márcia Alves Diniz, Ana Cláudia O Carreira, Carla Renata Sipert, Cindi Mie Uehara, Maria Stella Nunes Moreira, Laila Freire, Cibele Pelissari, Patrícia M Kossugue, Daniele Ribeiro de Araújo, Mari Cleide Sogayar, Márcia Martins Marques
Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo-responsive cell carrier (Pluronic® F-127) loaded with rhBMP4 and then photoactivated...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29214585/microphysiological-systems-tissue-chips-and-their-utility-for-rare-disease-research
#14
Lucie A Low, Danilo A Tagle
The scientific and technological development of microphysiological systems (MPS) modeling organs-on-chips, or "tissue chips" (TCs), has progressed rapidly over the past decade. Stem cell research and microfluidic concepts have combined to lead to the development of microphysiological platforms representing an ever-expanding list of different human organ systems. In the context of rare diseases, these bioengineered microfluidics platforms hold promise for modeling of disorders and could prove useful in the screening and efficacy testing of existing therapeutics...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29214379/dual-contribution-of-mesenchymal-stem-cells-employed-for-tissue-engineering-of-peripheral-nerves-trophic-activity-and-differentiation-into-connective-tissue-cells
#15
F Evaristo-Mendonça, A Carrier-Ruiz, R de Siqueira-Santos, R M P Campos, B Rangel, T H Kasai-Brunswick, V T Ribeiro-Resende
Adult peripheral nerves in vertebrates can regrow their axons and re-establish function after crush lesion. However, when there is extensive loss of a nerve segment, due to an accident or compressive damage caused by tumors, regeneration is strongly impaired. In order to overcome this problem, bioengineering strategies have been employed, using biomaterials formed by key cell types combined with biodegradable polymers. Many of these strategies are successful, and regenerated nerve tissue can be observed 12 weeks after the implantation...
December 7, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29211678/hemophilia-b-gene-therapy-with-a-high-specific-activity-factor-ix-variant
#16
Lindsey A George, Spencer K Sullivan, Adam Giermasz, John E J Rasko, Benjamin J Samelson-Jones, Jonathan Ducore, Adam Cuker, Lisa M Sullivan, Suvankar Majumdar, Jerome Teitel, Catherine E McGuinn, Margaret V Ragni, Alvin Y Luk, Daniel Hui, J Fraser Wright, Yifeng Chen, Yun Liu, Katie Wachtel, Angela Winters, Stefan Tiefenbacher, Valder R Arruda, Johannes C M van der Loo, Olga Zelenaia, Daniel Takefman, Marcus E Carr, Linda B Couto, Xavier M Anguela, Katherine A High
BACKGROUND: The prevention of bleeding with adequately sustained levels of clotting factor, after a single therapeutic intervention and without the need for further medical intervention, represents an important goal in the treatment of hemophilia. METHODS: We infused a single-stranded adeno-associated viral (AAV) vector consisting of a bioengineered capsid, liver-specific promoter and factor IX Padua (factor IX-R338L) transgene at a dose of 5×1011 vector genomes per kilogram of body weight in 10 men with hemophilia B who had factor IX coagulant activity of 2% or less of the normal value...
December 7, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/29209978/bioengineered-silver-nanoparticles-as-potent-anti-corrosive-inhibitor-for-mild-steel-in-cooling-towers
#17
Jayaraman Narenkumar, Punniyakotti Parthipan, Jagannathan Madhavan, Kadarkarai Murugan, Sreekar Babu Marpu, Anil Kumar Suresh, Aruliah Rajasekar
Silver nanoparticle-aided enhancement in the anti-corrosion potential and stability of plant extract as ecologically benign alternative for microbially induced corrosion treatment is demonstrated. Bioengineered silver nanoparticles (AgNPs) surface functionalized with plant extract material (proteinacious) was generated in vitro in a test tube by treating ionic AgNO3 with the leaf extract of Azadirachta indica that acted as dual reducing as well as stabilizing agent. Purity and crystallinity of the AgNPs, along with physical and surface characterizations, were evaluated by performing transmission electron microscopy, Fourier transform infrared spectroscopy, energy dispersive x-ray spectra, single-area electron diffractions, zeta potential, and dynamic light scattering measurements...
December 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29208899/in-vivo-transplantation-of-3d-encapsulated-ovarian-constructs-in-rats-corrects-abnormalities-of-ovarian-failure
#18
Sivanandane Sittadjody, Justin M Saul, John P McQuilling, Sunyoung Joo, Thomas C Register, James J Yoo, Anthony Atala, Emmanuel C Opara
Safe clinical hormone replacement (HR) will likely become increasingly important in the growing populations of aged women and cancer patients undergoing treatments that ablate the ovaries. Cell-based HRT (cHRT) is an alternative approach that may allow certain physiological outcomes to be achieved with lower circulating hormone levels than pharmacological means due to participation of cells in the hypothalamus-pituitary-ovary feedback control loop. Here we describe the in vivo performance of 3D bioengineered ovarian constructs that recapitulate native cell-cell interactions between ovarian granulosa and theca cells as an approach to cHRT...
December 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/29208552/controlling-human-corneal-stromal-stem-cell-contraction-to-mediate-rapid-cell-and-matrix-organization-of-real-architecture-for-3-dimensional-tissue-equivalents
#19
Dev Mukhey, James Phillips, Julie T Daniels, Alvena K Kureshi
The architecture of the human corneal stroma consists of a highly organized extracellular matrix (ECM) interspersed with keratocytes. Their progenitor cells; corneal stromal stem cells (CSSC) are located at the periphery, in the limbal stroma. A highly organized corneal ECM is critical for effective transmission of light but this structure may be compromised during injury or disease, resulting in loss of vision. Re-creating normal organization in engineered tissue equivalents for transplantation often involves lengthy culture times that are inappropriate for clinical use or utilisation of synthetic substrates that bring complications such as corneal melting...
December 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29208214/proof-of-concept-of-a-new-autologous-skin-substitute-for-the-treatment-of-deep-wounds-in-dogs
#20
L Ramió-Lluch, S Cerrato, P Brazis, R M Rabanal, D Fondevila, A Puigdemont
Autologous skin grafts are effective for the repair of large skin wounds, but the availability of large amounts of skin is often limited. Through bioengineering, several autologous skin substitutes have been developed for use in human clinical practice. However, few skin substitutes are available for use in animals. The aim of this study was to develop and assess an engineered autologous skin substitute for the treatment of deep wounds in veterinary medicine. Canine keratinocytes and fibroblasts were isolated after double enzyme digestion from 8mm punch biopsies from four healthy Beagle dogs...
December 2017: Veterinary Journal
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