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Jun-Yi Wu, Zhong-Xia Wang, Guang Zhang, Xian Lu, Guang-Hui Qiang, Wei Hu, An-Lai Ji, Jun-Hua Wu, Chun-Ping Jiang
Purpose: FTY720, known as fingolimod, is a new immunosuppressive agent with effective anticancer properties. Although it was recently confirmed that FTY720 inhibits cancer cell proliferation, FTY720 can also induce protective autophagy and reduce cytotoxicity. Blocking autophagy with Beclin 1 siRNA after treatment with FTY720 promotes apoptosis. The objective of this study was to enhance the anticancer effect of FTY720 in hepatocellular carcinoma (HCC) by targeted co-delivery of FTY720 and Beclin 1 siRNA using calcium phosphate (CaP) nanoparticles (NPs)...
2018: International Journal of Nanomedicine
Sergio Richter, Michael Döring, Micaela Ebert, Kerstin Bode, Andreas Müssigbrodt, Philipp Sommer, Daniela Husser, Gerhard Hindricks
Leadless cardiac pacemaker (LCP) therapy has been established clinically as a feasible and safe alternative to conventional transvenous pacemaker therapy for patients with an indication for single-chamber right-ventricular pacing.1-3 However, reports on loss of telemetry and pacing output due to abrupt battery failure called the safety of one of the two commercially available systems seriously into question. The initial battery advisory with the Nanostim™ LCP was issued by the manufacturer in October 2016, who instantly called a global stop to Nanostim™ implants...
March 14, 2018: Circulation
R Andler, S Hiessl, O Yücel, M Tesch, A Steinbüchel
Potential biotechnological recycling processes for rubber products include the bacterial degradation of poly(cis-1,4-isoprene) (IR) in order to achieve its total biodegradation or its biotransformation into useful products. The actinomycete Gordonia polyisoprenivorans strain VH2 catalyzes the degradation of IR and enables its use as a sole carbon source via β-oxidation. The initial cleavage reaction is catalyzed by the extracellular latex clearing protein (Lcp). This dioxygenase is the key enzyme for the formation of oligo(cis-1,4-isoprene) molecules with different lengths, i...
March 9, 2018: New Biotechnology
S Fateixa, M Raposo, H I S Nogueira, T Trindade
An important feature in the fabrication of surface-enhanced Raman scattering (SERS) platforms is, together with the high efficiency, to allow the rapid collection and analysis of a vestigial analyte. Conventional substrates based on rigid solid materials or metal hydrosols are not suitable for sample extraction, limiting their application in areas such as water quality monitoring. Herein, we have developed a strategy to fabricate SERS active substrates (Ag/LCP) based on liquid-crystal polymer (LCP) textile fibers decorated with Ag nanoparticles (NPs)...
May 15, 2018: Talanta
S Malm, S Maaß, U E Schaible, S Ehlers, S Niemann
LytR-cpsA-Psr (LCP) domain containing proteins fulfil important functions in bacterial cell wall synthesis. In Mycobacterium tuberculosis complex (Mtbc) strains, the causative agents of tuberculosis (TB), the genes Rv3484 and Rv3267 encode for LCP proteins which are putatively involved in arabinogalactan transfer to peptidoglycan. To evaluate the significance of Rv3484 for Mtbc virulence, we generated a deletion mutant in the Mtbc strain H37Rv and studied its survival in mice upon aerosol infection. The deletion mutant failed to establish infection demonstrating that Rv3484 is essential for growth in mice...
March 2, 2018: Scientific Reports
Nikola Bulatović, Miroslav Kezunović, Čedomir Vučetić, Nermin Abdić, Ivan Benčić, Tomislav Čengić
The rate of periprosthetic femoral fractures following total hip replacement has been growing steadily in the last 20 years and ranges from 0.1% to 2.1%. These fractures are mostly related to older patients with the presence of chronic diseases and frequently poor bone quality. The treatment is surgically very complex and demanding, followed by a series of complications. The evaluation in this retrospective study included 23 patients who were medically treated from January 2004 to December 2015 with the mean follow-up of 14...
September 2017: Acta Clinica Croatica
Lee-Anne Huber, Marko Rudar, Nathalie L Trottier, John P Cant, Cornelis F M de Lange
Twenty-eight lactating Yorkshire and Yorkshire × Landrace primiparous sows were used to test the hypothesis that feeding a diet with reduced CP concentration and supplemented with crystalline amino acids (CAA) does not decrease milk protein yield and litter growth but improves apparent N utilization for milk protein production. Sows were assigned to 1 of 2 dietary treatments: [1] Control (CON; 16.2% CP; analyzed content) or [2] Low CP with CAA to meet estimated requirements of limiting amino acids (LCP; 12...
February 19, 2018: Journal of Animal Science
Chairat Nawong, Kamontam Umsakul, Natthawan Sermwittayawong
An increasing production of natural rubber (NR) products has led to major challenges in waste management. In this study, the degradation of rubber latex gloves in a mineral salt medium (MSM) using a bacterial consortium, a mixed culture of the selected bacteria and a pure culture were studied. The highest 18% weight loss of the rubber gloves were detected after incubated with the mixed culture. The increased viable cell counts over incubation time indicated that cells used rubber gloves as sole carbon source leading to the degradation of the polymer...
February 3, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
M Pinheiro, C A Dobson, D Perry, M J Fagan
OBJECTIVES: Legg-Calvé-Perthes' disease (LCP) is an idiopathic osteonecrosis of the femoral head that is most common in children between four and eight years old. The factors that lead to the onset of LCP are still unclear; however, it is believed that interruption of the blood supply to the developing epiphysis is an important factor in the development of the condition. METHODS: Finite element analysis modelling of the blood supply to the juvenile epiphysis was investigated to understand under which circumstances the blood vessels supplying the femoral epiphysis could become obstructed...
February 2018: Bone & Joint Research
Jie Tang, Christopher B Howard, Stephen M Mahler, Kristofer J Thurecht, Leaf Huang, Zhi Ping Xu
The conjugation of ligands to nanoparticle platforms for the target delivery of therapeutic agents to the tumor tissue is one of the promising anti-cancer strategies. However, conventional nanoparticle platforms are not so effective in terms of the selectivity and transfection efficiency. In this study, we designed and developed a dual-target drug/gene delivery system based on lipid-coated calcium phosphate (LCP) nanoparticles (NPs) for significantly enhanced siRNA cellular uptake and transfection efficiency...
February 13, 2018: Nanoscale
Martin C Jordan, Konrad Fuchs, Timo M Heintel, Hendrik Jansen, Stefanie Hoelscher-Doht, Rainer H Meffert
PURPOSE: To compare fixed- or variable-angle locking screws in calcaneal fracture plates using a sawbones biomechanical model. METHODS: Intra-articular calcaneal fractures were created in synthetic bones. Reduction and stabilization was performed with three different plates. In Group A, a fixed-angle locking plate was used with screws fastened perpendicularly to the plate (3.5 LCP, DePuySynthes). In Groups B and C (2.7 VA-LCP, DePuySynthes and 3.5 Aptus, Medartis, respectively), fracture fixation was performed using variable-angle locking plates...
February 9, 2018: Journal of Orthopaedic Trauma
Manuel da Silva Pinheiro, Catherine Dobson, Nicholas M Clarke, Michael Fagan
Legg-Calvé-Perthes disease (LCP) is one of the most poorly understood diseases in paediatric orthopaedics. One common trait of LCP is the marked morphological difference between healthy and pathological hips, early deviations of which (i.e. prior to disease onset) have been suggested to lead to the overload and collapse of the epiphysis. Here, the impact of common variations in geometry is investigated with a finite element model of a juvenile femur under single leg standing and landing. Here, the impact of typical variations in geometry is investigated with a finite element model of a juvenile femur under single leg standing and landing...
February 8, 2018: Computer Methods in Biomechanics and Biomedical Engineering
Kaitlin Schaefer, Tristan Wold Owens, Daniel Kahne, Suzanne Walker
The Gram-positive bacterial cell wall is a large supramolecular structure and its assembly requires coordination of complex biosynthetic pathways. In the step that merges the two major biosynthetic pathways in Staphylococcus aureus cell wall as-sembly, conserved protein ligases attach wall teichoic acids to peptidoglycan, but the order of biosynthetic events is a longstanding question. Here, we use a chemical approach to define which of the possible peptidoglycan intermediates are substrates for wall-teichoic acid ligases, thereby establishing the order of cell wall assembly...
February 6, 2018: Journal of the American Chemical Society
Youngwoo Kim, Yong-Cheol Yoon, Jae-Woo Cho, Won-Tae Cho, Neung-Han Jeon, Chang-Wug Oh, Jong-Keon Oh
OBJECTIVE: To evaluate the effectiveness of an additional rim plate (3.5-mm pre-contoured locking compression plate) for stabilizing the posterolateral fragment in lateral tibial plateau fractures. METHODS: Standard lateral locking plates (either a PTP or PTLP) followed by the application of a secondary rim plate (VALCP) posterior laterally were applied to 40 matched pair knees from 20 fresh-frozen cadavers. RESULTS: The mean ratio of supported articular surface in the PTP group was 0...
January 5, 2018: Journal of Orthopaedic Trauma
Daniel Mandell, John Karbassi, Hanbing Zhou, Brian Burroughs, Philip Aurigemma, Abhay R Patel
INTRODUCTION: The treatment of end-stage first metatarso-phalangeal joint (MTP) arthritis has been arthrodesis. A dorsal non-locking plate with a lag screw has been the standard traditional fixation method. This study compares the biomechanical strength of a locking compression plate (LCP) with and without internal compression versus this known gold standard. METHODS: In group 1, six matched pairs of cadaver great toes were used to compare the standard non-locking dorsal plate and 3...
November 4, 2017: Foot
Yin-Sheng Wu, Bing Xu, Zuo-Qu Yu, Xiao-Feng Wang, Shou-Wang Dai, Min Li, Pei-Jian Tong
OBJECTIVE: To observe the biomechanical effects of the lateral wall of the femur in treating femoral intertrochanteric fractures with intramedullary or extramedullary fixation to guide the choice of clinical fixed methods. METHODS: Twelve adults femur specimens of intertrochanteric fractures were belong to the type A1 of the AO fracture classification and randomly divided into the lateral wall complete PFNA group, the lateral wall complete PF-LCP group, the lateral wall breakage PFNA group, lateral wall breakage PF-LCP group, every group had 3 specimens...
March 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
Joan Sargeant, Jocelyn M Lockyer, Karen Mann, Heather Armson, Andrew Warren, Marygrace Zetkulic, Sophie Soklaridis, Karen D Könings, Kathryn Ross, Ivan Silver, Eric Holmboe, Cindy Shearer, Michelle Boudreau
PURPOSE: The authors previously developed and tested a reflective model for facilitating performance feedback for practice improvement, the R2C2 model. It consists of four phases: relationship building, exploring reactions, exploring content, and coaching. This research studied the use and effectiveness of the model across different residency programs and the factors that influenced its effectiveness and use. METHOD: From July 2014-October 2016, case study methodology was used to study R2C2 model use and the influence of context on use within and across five cases...
January 16, 2018: Academic Medicine: Journal of the Association of American Medical Colleges
Sreekanth Kashayi-Chowdojirao, Aashish Vallurupalli, Vijay Krishna Chilakamarri, Chandrasekhar Patnala, Lalith Mohan Chodavarapu, Nageswara Rao Kancherla, Asif Hussain Khazi Syed
Background: Non-union humeral shaft fractures are seen frequently in clinical practice at about 2-10% in conservative management and 30% in surgically operated patients. Osteosynthesis using dynamic compression plate (DCP), intramedullary nailing, locking compression plate (LCP), Ilizarov technique along with bone grafting have been reported previously. In cases of prior failed plate-screw osteosynthesis the resultant osteopenia, cortical defect, bone loss, scalloping around screws and metallosis, make the management of non-union more complicated...
November 2017: Journal of Clinical Orthopaedics and Trauma
Kenji Tagashira, Kazuaki Z Takahashi, Jun-Ichi Fukuda, Takeshi Aoyagi
Liquid-crystal polymers (LCPs) are well known materials for functional sensor and actuators, because of their high-responsiveness to an electric field. Owing to their complex physical nature, however, the prediction of the functions of LCPs is a challenge. To attack this problem from a molecular point of view, a simulation study is a promising approach. In this work, for future applications of molecular dynamics simulations to problems involving an electric field, we develop an LCP model which consists of coarse-grained mesogenic molecules and smeared charges...
January 6, 2018: Materials
Prakash Rucktooa, Robert K Y Cheng, Elena Segala, Tian Geng, James C Errey, Giles A Brown, Robert M Cooke, Fiona H Marshall, Andrew S Doré
Here we report an efficient method to generate multiple co-structures of the A2A G protein-coupled receptor (GPCR) with small-molecules from a single preparation of a thermostabilised receptor crystallised in Lipidic Cubic Phase (LCP). Receptor crystallisation is achieved following purification using a low affinity "carrier" ligand (theophylline) and crystals are then soaked in solutions containing the desired (higher affinity) compounds. Complete datasets to high resolution can then be collected from single crystals and seven structures are reported here of which three are novel...
January 8, 2018: Scientific Reports
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