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protein nano-delivery

Shota Ichimizu, Hiroshi Watanabe, Hitoshi Maeda, Keisuke Hamasaki, Yuka Nakamura, Victor Tuan Giam Chuang, Ryo Kinoshita, Kento Nishida, Ryota Tanaka, Yuki Enoki, Yu Ishima, Akihiko Kuniyasu, Yoshihiro Kobashigawa, Hiroshi Morioka, Shiro Futaki, Masaki Otagiri, Toru Maruyama
Human serum albumin (HSA) is a superior carrier for delivering extracellular drugs. However, the development of a cell-penetrating HSA remains a great challenge due to its low membrane permeability. We report herein on the design of a series of palmitoyl-poly-arginine peptides (CPPs) and an evaluation of their cell-penetrating effects after forming a complex with HSA for use in intracellular drug delivery. The palmitoyl CPPs forms a stable complex with HSA by anchoring itself to the high affinity palmitate binding sites of HSA...
March 9, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Gunjan Vasant Bonde, Sarita Kumari Yadav, Sheetal Chauhan, Pooja Mittal, Gufran Ajmal, Sathish Thokala, Brahmeshwar Mishra
Breast cancer stands the second prominent cause of death among women. For its efficient treatment, Lapatinib (LAPA) was developed as a selective tyrosine kinase inhibitor of receptors, overexpressed by breast cancer cells. Various explored delivery strategies for LAPA indicated its controlled release with enhanced aqueous solubility, improved bioavailability, decreased plasma protein binding, reduced dose and toxicity to the other organs with maximized clinical efficacy, compared to its marketed tablet formulation...
March 9, 2018: Expert Opinion on Drug Delivery
Peter Hart, Alastair Copland, Gil Reynolds Diogo, Shane Harris, Ralf Spallek, Wulf Oehlmann, Mahavir Singh, Juan Basile, Martin Rottenberg, Matthew John Paul, Rajko Reljic
Tuberculosis (TB) is the leading cause of death from infectious disease, and the current vaccine, Bacillus Calmette-Guerin (BCG), is inadequate. Nanoparticles (NPs) are an emerging vaccine technology, with recent successes in oncology and infectious diseases. NPs have been exploited as antigen delivery systems and also for their adjuvantic properties. However, the mechanisms underlying their immunological activity remain obscure. Here, we developed a novel mucosal TB vaccine (Nano-FP1) based upon yellow carnauba wax NPs (YC-NPs), coated with a fusion protein consisting of three Mycobacterium tuberculosis (Mtb) antigens: Acr, Ag85B, and HBHA...
March 7, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
Jing-Min Liu, Dong-Dong Zhang, Guo-Zhen Fang, Shuo Wang
Combination of biological entities with functional nanostructure would produce the excellent systemic drug-delivery vehicles that possess the ability to cross the biological barriers. Herein, from a biomimetic point of view, erythrocyte membrane bioinspired optical nanocarrier is fabricated by integrating Red blood cell (RBC) membrane vesicle with near-infrared persistent luminescence nanophosphors (PLNPs). The triple-doped zinc gallogermanate nanostructures with super-long near-infrared persistent luminescence (ZGGO) are used as optical emission center, mesoporous silica coated on the PLNPs (ZGGO@mSiO2 ) is employed for drug delivery, and the RBC membrane vesicle is introduced for biomimetic functionalization to ensure the developed nanocarriers bypass macrophage uptake and systemic clearance...
February 23, 2018: Biomaterials
Sharon M Sagnella, Jennifer Trieu, Himanshu Brahmbhatt, Jennifer A MacDiarmid, Alex MacMillan, Renee M Whan, Christopher M Fife, Joshua A McCarroll, Andrew J M Gifford, David S Ziegler, Maria Kavallaris
Advanced stage neuroblastoma is an aggressive disease with limited treatment options for patients with drug resistant tumors. Targeted delivery of chemotherapy for pediatric cancers offers promise to improve treatment efficacy and reduce toxicity associated with systemic chemotherapy. The EnGeneIC delivery vehicle (EDVTM) is a nanocell which can package chemotherapeutic drugs and target tumors via attachment of bispecific proteins to the surface of the nanocell. Phase 1 trials in adults with refractory tumors have shown an acceptable safety profile...
February 28, 2018: Molecular Cancer Therapeutics
Ying Hao, Wei Li, XingLi Zhou, Fan Yang, ZhiYong Qian
A microneedle (MN) is a micron-sized needle with a height of 10-2000 μm and a width of 10-50 μm, which can penetrate through the epidermis layer to dermal tissue directly without pain. Microneedles are widely used in transdermal drug delivery systems (TDDS) because they are efficient, safe, convenient and painless. Morphologically, microneedles are divided into four types: solid microneedles, coated microneedles, dissolving microneedles, and hollow microneedles. Different types of microneedles play different roles in different research fields...
December 1, 2017: Journal of Biomedical Nanotechnology
Christopher D Spicer, Coline Jumeaux, Bakul Gupta, Molly M Stevens
Peptide- and protein-nanoparticle conjugates have emerged as powerful tools for biomedical applications, enabling the treatment, diagnosis, and prevention of disease. In this review, we focus on the key roles played by peptides and proteins in improving, controlling, and defining the performance of nanotechnologies. Within this framework, we provide a comprehensive overview of the key sequences and structures utilised to provide biological and physical stability to nano-constructs, direct particles to their target and influence their cellular and tissue distribution, induce and control biological responses, and form polypeptide self-assembled nanoparticles...
February 26, 2018: Chemical Society Reviews
Giuditta Dal Cortivo, Gabriel E Wagner, Paolo Cortelletti, Krishna Mohan Padmanabha Das, Klaus Zangger, Adolfo Speghini, Daniele Dell'Orco, N Helge Meyer
Nanoparticles have been recognized as promising tools for targeted drug-delivery and protein therapeutics. However, the mechanisms of protein-nanoparticle interaction and the dynamics underlying the binding process are poorly understood. Here, we present a general methodology for the characterization of protein-nanoparticle interaction on a molecular level. To this end we combined biophysical techniques including nuclear magnetic resonance (NMR), circular dichroism (CD), resonance energy transfer (RET) and surface plasmon resonance (SPR)...
February 21, 2018: Scientific Reports
Santosh K Misra, Taylor L Kampert, Dipanjan Pan
Pathogenesis of many cancer is driven by the Myc oncoprotein, its dimerization with Max, and the binding of this heterodimer to E-Boxes of target genes. The systemic effectiveness of potent small molecule inhibitors of Myc-Max dimerization has been lim-ited by poor bioavailability, rapid metabolism, and inadequate target site penetration. The potential of gene therapy for target-ing Myc can be fully realized by successful synthesis of a smart cargo. We developed a 'nuclein' type nanoparticle "siNozyme" (45±5 nm) from nano-assembly of pamitoyl-bio-conjugated acetyl co-enzyme-A for stable incorporation of chemotherapeutics and biologics to achieve remarkable growth inhibition of human melanoma...
February 21, 2018: Bioconjugate Chemistry
Wenwu Xiao, Qingtao Li, Huimin He, Wenxiu Li, Xiaodong Cao, Hua Dong
Precise manipulation of biomolecules distribution and functions via biomolecule-matrix interaction is very important and challenging for tissue engineering and regenerative medicine. As a well-known biomimetic matrix, electrospun fibers often lack the unique spatial complexity compared to their natural counterparts in vivo and thus cannot deliver fully the regulatory cues to biomolecules. In this paper, we report a facile and reliable method to fabricate micro- and nano-structured poly (l-lactic acid) (PLLA) fibrous matrices with spatial complexity by combination of advanced electrospinning and agarose hydrogel stamp based micropatterning...
February 20, 2018: ACS Applied Materials & Interfaces
Chengxu Cai, Mingzhe Wang, Liming Wang, Bing Wang, Weiyue Feng, Chunying Chen
Characterization of bio-nano interface is crucial for developing safer and more efficient nanoparticles in nanomedical application. PEGylation is commonly used in nanocarrier for drug delivery, as it confers nanoparticles good stability, stealth effect and better targeting specificity compared with those without PEGylation. However, the protein binding state on PEGylated AuNP is still limited known. In present work, we prepared 13 nm AuNPs and then PEGylated them with thiol PEG methoxy. Lysozyme is selected as a model protein and to investigate the interactions on protein-PEGylated/AuNP interface...
August 1, 2018: Journal of Nanoscience and Nanotechnology
Song-Pei Li, Zhong-Xiao Lin, Xue-Yan Jiang, Xi-Yong Yu
Exosomes are nano-sized vesicles that serve as mediators for intercellular communication through the delivery of cargo, including protein, lipids, nucleic acids or other cellular components, to neighboring or distant cells. Exosomal cargo may vary in response to different physiological or pathological conditions. The endosomal sorting complex required for transport (ESCRT) family has been widely accepted as a key mechanism in biogenesis and cargo sorting. On the other hand, accumulating evidence show that ESCRT-independent pathways exist...
February 8, 2018: Acta Pharmacologica Sinica
Sheng-Jen Cheng, Hsien-Yi Chiu, Priyank V Kumar, Kuan Yu Hsieh, Jia-Wei Yang, You-Rong Lin, Yu-Chih Shen, Guan-Yu Chen
Graphene oxide (GO), a derivative of graphene, and its related nanomaterials have attracted much attention in recent years due to the excellent biocompatibility and large surface area of GO with abundant oxygen functional groups, which further enable it to serve as a nano-bio interface. Herein, we demonstrate the induction of blue fluorescence in GO suspensions via a mild thermal annealing procedure. Additionally, this procedure preserves the oxygen functional groups on the graphene plane which enables the conjugation of cancer drugs without obvious cytotoxicity...
February 8, 2018: Biomaterials Science
Derfogail Delcassian, Asha K Patel, Abel B Cortinas, Robert Langer
Over the last century there has been a dramatic change in the nature of therapeutic, biologically active molecules available to treat disease. Therapies have evolved from extracted natural products towards rationally designed biomolecules, including small molecules, engineered proteins, and nucleic acids. The use of potent drugs which target specific organs, cells, or biochemical pathways, necessitates new tools which can enable controlled delivery and dosing of these therapeutics to their biological targets...
February 8, 2018: Journal of Drug Targeting
L A Avila, R Chandrasekar, K E Wilkinson, J Balthazor, M Heerman, J Bechard, S Brown, Y Park, S Dhar, G R Reeck, J M Tomich
Development of new and specific insect pest management methods is critical for overcoming pesticide resistance and collateral off-target killings. Gene silencing by feeding dsRNA to insects shows promise in this area. Here we described the use of a peptide nano-material, branched amphiphilic peptide capsules (BAPCs), that facilitates cellular uptake of dsRNA by insects through feeding. The insect diets included dsRNA with and without complexation with BAPCs. The selected insect species come from two different orders with different feeding mechanisms: Tribolium castaneum and Acyrthosiphon pisum...
January 31, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Vincent Fiegel, Sébastien Harlepp, Sylie Bégin-Colin, Dominique Bégin, Damien Mertz
One key challenge in the fields of nanomedecine and tissue engineering is the design of theranostic nanoplatforms able to monitor their therapeutic effect by imaging. Among current developed nano-objects, carbon nanotubes (CNTs) were found suitable to combine imaging, photothermal therapy and to be loaded with hydrophobic drugs. However, a main problem is their resulting low hydrophilicity. To face this problem, an innovative method is developed here, which consists in loading the surface of carbon nanotubes (CNTs) with drugs followed by a protein coating around them...
January 25, 2018: Chemistry: a European Journal
Mengrui Liu, Hongliang Du, Abdur Rauf Khan, Jianbo Ji, Aihua Yu, Guangxi Zhai
In this report, redox/enzyme responsive chondroitin sulfate-ss-deoxycholic acid (CSCD) conjugates were synthesized using cystamine as the linkage which could self-assemble to form self-assembled nanoparticles (175.6 + 5.2 nm) in the aqueous environment. Docetaxel (DTX) was loaded in nanoparticles with desired loading efficiency for the inhibition of tumor growth and metastasis of melanoma. Interestingly, nanoparticles were demonstrated to respond to hyaluronidase-1 (Hyal-1) which could degrade chondroitin sulfate (CS) backbones...
March 15, 2018: Carbohydrate Polymers
Chaofeng Liang, Weitong Sun, Haiyong He, Baoyu Zhang, Cong Ling, Bocheng Wang, Tengchao Huang, Bo Hou, Ying Guo
Introduction: MiR-135a is found to selectively induce apoptosis in glioma cell but not in normal neurons and glial cells. However, low transfection efficacy limits its application in vivo as other miRNAs. We prepared a new kind of nano-vector based on polyethylene glycol methyl ether (mPEG) and hyper-branched polyethylenimine (hy-PEI) in order to improve the miRNA delivery system into the glioma cells. Methods: The mPEG-g-PEI/miR-135a was constructed and detected by 1H NMR and FTIR analyses...
2018: International Journal of Nanomedicine
Yanan Shi, Xinfeng Sun, Liping Zhang, Kaoxiang Sun, Keke Li, Youxin Li, Qiang Zhang
To improve the oral efficiency of exenatide, we prepared polyethylene glycol-poly(lactic-co-glycolic acid) (PEG-PLGA) NPs modified with Fc (NPs-Fc) for exenatide oral delivery. Exenatide was encapsulated into the NPs by the w/o/w emulsion-solvent evaporation method. The particle size of the NPs-Fc was approximately 30 nm larger than that of the unmodified NPs with polydispersity indices in a narrow range (PDIs; PDI < 0.3) as detected by DLS, and the highest encapsulation efficiency of exenatide in the NPs was greater than 80%...
January 15, 2018: Scientific Reports
Tao Zhang, Ruyi Yang, Shengnan Yang, Jibin Guan, Dong Zhang, Yan Ma, Hongzhuo Liu
Polymer nano-sized hydrogels (nanogels) as drug delivery carriers have been investigated over the last few decades. Pullulan, a nontoxic and nonimmunogenic hydrophilic polysaccharide derived from fermentation of black yeast like Aureobasidium pullulans with great biocompatibility and biodegradability, is one of the most attractive carriers for drug delivery systems. In this review, we describe the preparation, characterization, and 'switch-on/off' mechanism of typical pullulan self-assembled nanogels (self-nanogels), and then introduce the development of hybrid hydrogels that are numerous resources applied for regenerative medicine...
November 2018: Drug Delivery
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