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protein nano-delivery

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https://www.readbyqxmd.com/read/28323095/megalin-targeted-enhanced-transfection-efficiency-in-cultured-human-hk-2-renal-tubular-proximal-cells-using-aminoglycoside-carboxyalkyl-polyethylenimine-containing-nanoplexes
#1
Fatemeh Oroojalian, Ali Hossein Rezayan, Wayne Thomas Shier, Khalil Abnous, Mohammad Ramezani
Non-viral vectors are of interest as therapeutic gene delivery agents in gene therapy, because they are simple to prepare, easy to modify and have definable safety profiles compared to viral vectors. The potential of gene therapy in the treatment of renal diseases is limited by a lack of effective kidney-targeted gene delivery systems. Aminoglycoside antibiotics gentamicin and neomycin were connected by amide linkages to carboxyl groups on carboxyalkylated-PEI25 (25kDa PEI) or carboxyalkylated-PEI10 (10kDa PEI)...
March 16, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28276530/overcoming-sorafenib-evasion-in-hepatocellular-carcinoma-using-cxcr4-targeted-nanoparticles-to-co-deliver-mek-inhibitors
#2
Yunching Chen, Ya-Chi Liu, Yun-Chieh Sung, Rakesh R Ramjiawan, Ts-Ting Lin, Chih-Chun Chang, Kuo-Shyang Jeng, Chiung-Fang Chang, Chun-Hung Liu, Dong-Yu Gao, Fu-Fei Hsu, Annique M Duyverman, Shuji Kitahara, Peigen Huang, Simona Dima, Irinel Popescu, Keith T Flaherty, Andrew X Zhu, Nabeel Bardeesy, Rakesh K Jain, Cyril H Benes, Dan G Duda
Sorafenib is a RAF inhibitor approved for several cancers, including hepatocellular carcinoma (HCC). Inhibition of RAF kinases can induce a dose-dependent "paradoxical" upregulation of the downstream mitogen-activated protein kinase (MAPK) pathway in cancer cells. It is unknown whether "paradoxical" ERK activation occurs after sorafenib therapy in HCC, and if so, if it impacts the therapeutic efficacy. Here, we demonstrate that RAF inhibition by sorafenib rapidly leads to RAF dimerization and ERK activation in HCCs, which contributes to treatment evasion...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28275313/zinc-oxide-tetrapods-inhibit-herpes-simplex-virus-infection-of-cultured-corneas
#3
Neil Duggal, Dinesh Jaishankar, Tejabhiram Yadavalli, Satvik Hadigal, Yogendra Kumar Mishra, Rainer Adelung, Deepak Shukla
PURPOSE: Infection of the human cornea by herpes simplex virus type-1 (HSV-1) can cause significant vision loss. The purpose of this study was to develop an ex vivo model to visualize viral growth and spread in the cornea. The model was also used to analyze cytokine production and study the antiviral effects of zinc oxide tetrapods. METHODS: A β-galactosidase-expressing recombinant virus, HSV-1(KOS)tk12, was used to demonstrate the ability of the virus to enter and develop blue plaques on human corneal epithelial (HCE) cells and corneal tissues...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28249594/enhancement-of-scutellarin-oral-delivery-efficacy-by-vitamin-b12-modified-amphiphilic-chitosan-derivatives-to-treat-type-ii-diabetes-induced-retinopathy
#4
Jingnan Wang, Jiayun Tan, Jiahao Luo, Peilin Huang, Wuyi Zhou, Luming Chen, Lingli Long, Li-Ming Zhang, Banghao Zhu, Liqun Yang, David Y B Deng
BACKGROUND: Diabetic retinopathy is the most common complication in diabetic patients relates to high expression of VEGF and microaneurysms. Scutellarin (Scu) turned out to be effective against diabetes related vascular endothelial cell dysfunction. However, its clinical applications have been limited by its low bioavailability. In this study, we formulated and characterized a novel intestinal target nanoparticle carrier based on amphiphilic chitosan derivatives (Chit-DC-VB12) loaded with scutellarin to enhance its bioavailability and then evaluated its therapeutic effect in experimental diabetic retinopathy model...
March 1, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28249113/fabrication-of-a-soybean-bowman-birk-inhibitor-bbi-nanodelivery-carrier-to-improve-bioavailability-of-curcumin
#5
Chun Liu, Fenfen Cheng, Xiaoquan Yang
Curcumin is a poorly water-soluble drug, and its oral bioavailability is very low. Here, a novel self-assembly nanoparticle delivery carrier has been successfully developed by using soybean Bowman-Birk inhibitor (BBI) to improve the solubility, bioaccessibility, and oral absorption of curcumin. BBI is a unique protein, which can be resistant to the pH range and proteolytic enzymes in the gastrointestinal tract (GIT), bioavailable, and not allergenic. The encapsulation efficiencies (EE) and the loading capacities (LC) of curcumin in the curcumin-loaded BBI nanoparticles (Cur-BBI-NPs, size = 90...
March 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28248524/biocompatible-soft-nanoparticles-with-multiple-morphologies-obtained-from-nanoprecipitation-of-amphiphilic-graft-copolymers-in-a-backbone-selective-solvent
#6
Gaëlle Le Fer, Clémence Le Cœur, Jean-Michel Guigner, Catherine Amiel, Gisèle Volet
Stealth nanocarriers are a promising technology for the treatment of diseases. However, the preparation and characterization of well-defined soft nanoparticulate systems remain challenging. Here we describe a platform of amphiphilic graft copolymers leading to nanoparticles with multiple morphologies and the role of the hydrophilic backbone in their interaction with a model protein. The amphiphilic graft copolymers platform was composed of hydrophilic backbone poly(2-methyl-2-oxazoline-co-2-pentyl-2-oxazoline) (P(MeOx-co-PentOx)), prepared via cationic ring-opening polymerization...
March 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28244542/probing-the-structural-basis-and-adsorption-mechanism-of-an-enzyme-on-nano-sized-protein-carriers
#7
Yanxiong Pan, Sunanda Neupane, Jasmin Farmakes, Michael Bridges, James Froberg, Jiajia Rao, Steven Y Qian, Guodong Liu, Yongki Choi, Zhongyu Yang
Silica nanoparticles (SiNPs) are important nano-sized, solid-state carriers/hosts to load, store, and deliver biological or pharmaceutical cargoes. They are also good potential solid supports to immobilize proteins for fundamental protein structure and dynamics studies. However, precaution is necessary when using SiNPs in these areas because adsorption might alter the activity of the cargoes, especially when enzymes are loaded. Therefore, it becomes important to understand the structural basis of the cargo enzyme activity changes, if there is any...
February 28, 2017: Nanoscale
https://www.readbyqxmd.com/read/28243085/continuous-release-of-bone-morphogenetic-protein-2-through-nano-graphene-oxide-based-delivery-influences-the-activation-of-the-nf-%C3%AE%C2%BAb-signal-transduction-pathway
#8
Cheng Zhong, Jun Feng, Xiangjin Lin, Qi Bao
Graphene oxide (GO) has been used as a delivery vehicle for small molecule drugs and nucleotides. To further investigate GO as a smart biomaterial for the controlled release of cargo molecules, we hypothesized that GO may be an appropriate delivery vehicle because it releases bone morphogenetic protein 2 (BMP2). GO characterization indicated that the size distribution of the GO flakes ranged from 81.1 nm to 45,749.7 nm, with an approximate thickness of 2 nm. After BMP2 adsorption onto GO, Fourier-transformed infrared spectroscopy (FTIR) and thermal gravimetric analysis were performed...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28238134/evaluating-nanoparticle-binding-to-blood-compartment-immune-cells-in-high-throughput-with-flow-cytometry
#9
Shann S Yu
Nanoparticles are increasingly being utilized for in vivo applications, where they are implemented as carriers for drugs, contrast agents for noninvasive medical imaging, or delivery vehicles for macromolecular agents such as DNA or proteins. However, they possess many physical and chemical properties that cause them to become rapidly recognized by the immune system as a foreign body, leading to their clearance and elimination, even before they may accumulate to critical concentrations at anatomic and cellular sites of action...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28237436/cationic-dendritic-starch-as-a-vehicle-for-photodynamic-therapy-and-sirna-co-delivery
#10
Sarah A Engelberth, Nadine Hempel, Magnus Bergkvist
Cationic enzymatically synthesized glycogen (cESG) is a naturally-derived, nano-scale carbohydrate dendrite that has shown promise as a cellular delivery vehicle owing to its flexibility in chemical modifications, biocompatibility and relative low cost. In the present work, cESG was modified and evaluated as a vehicle for tetraphenylporphinesulfonate (TPPS) in order to improve cellular delivery of this photosensitizer and investigate the feasibility of co-delivery with short interfering ribonucleic acid (siRNA)...
February 20, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28228075/challenges-in-dermal-delivery-of-therapeutic-antimicrobial-protein-and-peptides
#11
Rohan Aminbhai Lalani, Ambikanandan Misra, Jitendra Amrutiya, Hinal Patel, Priyanka Bhatt, Vivek Patel
BACKGROUND: Protein and peptides in biological system form an important part of innate immune system and are being explored for potential use in various diseases as therapeutics. Importance of proteins and peptides as a new class of antimicrobial agents have boosted research in the field of biotechnology as potential alternative to antibiotic agents. OBJECTIVE: Protein and peptides antimicrobial as a therapeutics class are structurally diverse and exhibit potent activity against microbes by various mechanisms...
February 22, 2017: Current Drug Metabolism
https://www.readbyqxmd.com/read/28215090/nonviral-genome-editing-based-on-a-polymer-derivatized-crispr-nanocomplex-for-targeting-bacterial-pathogens-and-antibiotic-resistance
#12
Yoo Kyung Kang, Kyu Kwon, Jea Sung Ryu, Ha Neul Lee, Chankyu Park, Hyun Jung Chung
The overuse of antibiotics plays a major role in the emergence and spread of multidrug-resistant bacteria. A molecularly targeted, specific treatment method for bacterial pathogens can prevent this problem by reducing the selective pressure during microbial growth. Herein, we introduce a nonviral treatment strategy delivering genome editing material for targeting antibacterial resistance. We apply the CRISPR-Cas9 system, which has been recognized as an innovative tool for highly specific and efficient genome engineering in different organisms, as the delivery cargo...
February 27, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28157292/structurally-programmed-assembly-of-translation-initiation-nanoplex-for-superior-mrna-delivery
#13
Jiahe Li, Wade Wang, Yanpu He, Yingzhong Li, Emily Z Yan, Ketian Zhang, Darrell J Irvine, Paula T Hammond
Messenger RNA (mRNA) represents a promising class of nucleic-acid-based therapeutics. While numerous nanocarriers have been developed for mRNA delivery, the inherent labile nature of mRNA results in a very low transfection efficiency and poor expression of desired protein. Here we preassemble the mRNA translation initiation structure through an inherent molecular recognition between 7-methylguanosine (m(7)G)-capped mRNA and eukaryotic initiation factor 4E (eIF4E) protein to form ribonucleoproteins (RNPs), thereby mimicking the first step of protein synthesis inside cells...
February 14, 2017: ACS Nano
https://www.readbyqxmd.com/read/28139723/single-%C3%AE-particle-irradiation-permits-real-time-visualization-of-rnf8-accumulation-at-dna-damaged-sites
#14
Giovanna Muggiolu, Michal Pomorski, Gérard Claverie, Guillaume Berthet, Christine Mer-Calfati, Samuel Saada, Guillaume Devès, Marina Simon, Hervé Seznec, Philippe Barberet
As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28137239/targeted-theranostics-against-solid-cancer-using-metal-bond-milk-protein-and-aptamers
#15
Suman Chaudhary, Rupinder Kaur Kanwar, Jagat Rakesh Kanwar
Targeted therapeutics is a new generation therapy that can increase the efficacy of chemotherapeutic drugs, by facilitating site-specific delivery with minimum off-target effects. Amongst the targeted therapy, nucleic-acid based aptamers are increasingly gaining interest due to their small size, long shelf-life and ease in synthesis. Further, lactoferrin, a milk protein belonging to the transferrin family is now an established multi-functional iron-binding protein. Its applicability as an immunomodulator, antimicrobial and an anti-cancer agent has made this protein highly valuable...
January 30, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28123631/non-invasive-administration-of-biodegradable-nano-carrier-vaccines
#16
REVIEW
Mohd Abul Kalam, Abdul Arif Khan, Aws Alshamsan
Polymeric nanoparticulate carriers play an important role and holding a significant potential for the development of novel immunomodulatory agents as easily they are taken up by antigen presenting cells. They allow an enhanced antigen stability, better immunogenicity and immunostimulatory effect with sustained and controlled release of the antigen to the target sites. Better information and vital understanding of mechanism of action, interaction of such vectors with the APCs and dendritic cells and antigen release kinetics in immunomodulatory effects, and improved knowledge of their in vivo fate and distribution are now needed, those collectively would speed up the rational strategies of nanoparticles as carriers for vaccines and other protein antigens...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28120725/bile-salt-stabilized-vesicles-bilosomes-a-novel-nano-pharmaceutical-design-for-oral-delivery-of-proteins-and-peptides
#17
Javed Ahmad, Madhur Singhal, Saima Amin, Md Rizwanullah, Sohail Akhter, Mohammad Amjad Kamal, Nafis Haider, Patrick Midoux, Chantal Pichon
With the advent of novel vesicular drug delivery systems especially bilosomes, for large molecular weight proteins and peptides, their oral administration seems a viable approach. These nano-vesicles have shown promising results for the effective delivery of insulin and other therapeutics, perhaps due to their structural composition. The present review has elaborated the biopharmaceutical challenges for the oral delivery of therapeutic proteins and peptides as well as presented a novel approach to deliver the essential macromolecules through oral route as bilosomes...
January 24, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28116676/scientific-and-regulatory-considerations-for-generic-complex-drug-products-containing-nanomaterials
#18
Nan Zheng, Dajun D Sun, Peng Zou, Wenlei Jiang
In the past few decades, the development of medicine at the nanoscale has been applied to oral and parenteral dosage forms in a wide range of therapeutic areas to enhance drug delivery and reduce toxicity. An obvious response to these benefits is reflected in higher market shares of complex drug products containing nanomaterials than that of conventional formulations containing the same active ingredient. The surging market interest has encouraged the pharmaceutical industry to develop cost-effective generic versions of complex drug products based on nanotechnology when the associated patent and exclusivity on the reference products have expired...
January 23, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28116599/nano-reservoir-bioadhesive-tablets-enhance-protein-drug-permeability-across-the-small-intestine
#19
Lifang Yin, Yong Wang, Cuifeng Wang, Min Feng
Most therapeutic proteins are classified as class III drugs according to the Biopharmaceutical Classification System means that the low permeability across the intestinal epithelium is the rate-limited step for their oral absorption. Cationic chitosan nanoparticles have been found to open the tight junctions between epithelial cells. On the other hand, bioadhesive delivery devices could prolong the gastrointestinal residence time. In the present study, we developed a novel nano-reservoir bioadhesive tablets that combining the advantages of cationic nanoparticles and bioadhesive delivery devices anticipated achieving effective transport of sufficient protein drugs across the intestinal epithelium...
January 23, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28112546/characteristics-and-roles-of-exosomes-in-cardiovascular-disease
#20
Yuan Zhang, Yan-Wei Hu, Lei Zheng, Qian Wang
Exosomes are nano-sized biological membrane-enclosed vesicles that contain a cell-specific cargo of proteins, lipids, and nucleic acids that are released and taken up by most cell types, thereby inducing expression and functional changes via horizontal transfer of cargos between cells. Thus, exosomes present a largely unknown "cell-to-cell" communication system, which is now increasingly being investigated for diagnostic and therapeutic use in cardiovascular disease (CVD). The purpose of this review is to summarize recent findings on the properties and roles of exosomes in a variety of physiological and pathological settings related to CVD...
March 2017: DNA and Cell Biology
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