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Xiyan Sun, Chao Zhang, Yang Cao, Erbiao Liu
MiR-150 has been demonstrated to inhibit tumor progression in various human cancers, including colorectal cancer, ovarian cancer and thyroid cancer. However, the role of miR-150 in melanoma remains to be determined. In this study, we found that miR-150 was underexpressed in melanoma tissues and cell lines. Through transfection of miR-150 mimics, we found that miR-150 significantly inhibited the proliferation, migration and invasion of melanoma cells. In mechanism, we found that MYB was a target of miR-150 in melanoma cells...
April 24, 2018: Oncology Research
Na Yao, Li Jing, Han Zheng, Min Chen, Ye Shen
Jasmonates, as a plant endogenous hormone, can induce the biosynthesis of terpenoids, alkaloids and flavonoids and other medicinal active ingredients, and play an important role in the plant secondary metabolic process. The tanscription factors can activate the expression of multiple genes in the biosynthesis of plant secondary metabolites by binding the cis-elements of the target genes. Then, it effectively activates or inhibits the activities of the enzymes on the biosynthesis of plant secondary metabolites, further regulate specific biosynthesis and accumulation of secondary metabolites...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Xiaofeng Liu, Yunxiao Xu, Liping Han, Yan Yi
Transcription factor MYB is essential for the tumorigenesis of multiple cancers, especially leukemia, breast cancer, colon cancer, adenoid cystic carcinoma and brain cancer. Thus, MYB has been regarded as an attractive target for tumor therapy. However, pioneer studies of antisense oligodeoxynucleotides against MYB, which were launched three decades ago in leukemia therapy, were discontinued because of their unsatisfactory clinical outcomes. In recent years, the roles of MYB in tumor transformation have become increasingly clear...
2018: Journal of Cancer
Yanzhao Zhang, Shuzhen Xu, Yanwei Cheng, Zhengfeng Peng, Jianming Han
Red leaf lettuce ( Lactuca sativa L.) is popular due to its high anthocyanin content, but poor leaf coloring often occurs under low light intensity. In order to reveal the mechanisms of anthocyanins affected by light intensity, we compared the transcriptome of L. sativa L. var. capitata under light intensities of 40 and 100 μmol m-2 s-1 . A total of 62,111 unigenes were de novo assembled with an N50 of 1,681 bp, and 48,435 unigenes were functionally annotated in public databases. A total of 3,899 differentially expressed genes (DEGs) were detected, of which 1,377 unigenes were up-regulated and 2,552 unigenes were down-regulated in the high light samples...
2018: PeerJ
Takahiro Nishiyama, Yuichi Ishikawa, Naomi Kawashima, Akimi Akashi, Yoshiya Adachi, Hikaru Hattori, Yoko Ushijima, Hitoshi Kiyoi
We analyzed the genetic mutation status of 13 patients with therapy-related myeloid neoplasms (t-MN). Consistent with previous reports, t-MN cells preferentially acquired mutations in TP53 and epigenetic modifying genes, instead of mutations in tyrosine kinase and spliceosome genes. Furthermore, we compared the mutation status of three t-MN cells with each of the initial lymphoid malignant cells, and identified common mutations among t-MN and the initial malignant cells in two patients. In a patient who developed chronic myelomonocytic leukemia (CMML) after follicular lymphoma (FL), TET2 mutation was identified in both CMML and FL cells...
April 2018: Cancer Genetics
Mei-Yu Sun, Jing-Yi Li, Dong Li, Feng-Jie Huang, Di Wang, Hui Li, Quan Xing, Hui-Bin Zhu, Lei Shi
Drynaria roosii (Nakaike) is a traditional Chinese medicinal fern, known as 'GuSuiBu'. The corresponding effective components of naringin/neoeriocitrin share highly similar chemical structure and medicinal function. Our HPLC-MS/MS results showed that the accumulation of naringin/neoeriocitrin depended on specific tissues or ages. However, little was known about the expression patterns of naringin/neoeriocitrin related genes involved in their regulatory pathways. For lack of the basic genetic information, we applied a combination of SMRT sequencing and SGS to generate the complete and full-length transcriptome of D...
April 12, 2018: Plant & Cell Physiology
Anya Kalsbeek, Jenna Veenstra, Jason Westra, Craig Disselkoen, Kristin Koch, Katelyn A McKenzie, Jacob O'Bott, Jason Vander Woude, Karen Fischer, Greg C Shearer, William S Harris, Nathan L Tintle
Recent analyses have suggested a strong heritable component to circulating fatty acid (FA) levels; however, only a limited number of genes have been identified which associate with FA levels. In order to expand upon a previous genome wide association study done on participants in the Framingham Heart Study Offspring Cohort and FA levels, we used data from 2,400 of these individuals for whom red blood cell FA profiles, dietary information and genotypes are available, and then conducted a genome-wide evaluation of potential genetic variants associated with 22 FAs and 15 FA ratios, after adjusting for relevant dietary covariates...
2018: PloS One
Jessica E Bolden, Erin C Lucas, Geyu Zhou, Jeremy A O'Sullivan, Carolyn A de Graaf, Mark D McKenzie, Ladina Di Rago, Tracey M Baldwin, Jake Shortt, Warren S Alexander, Bruce S Bochner, Matthew E Ritchie, Douglas J Hilton, Kirsten A Fairfax
In recent years multi-parameter flow cytometry has enabled identification of cells at major stages in myeloid development; from pluripotent hematopoietic stem cells, through populations with increasingly limited developmental potential (common myeloid progenitors and granulocyte-macrophage progenitors), to terminally differentiated mature cells. Myeloid progenitors are heterogeneous, and the surface markers that define transition states from progenitors to mature cells are poorly characterized. Siglec-F is a surface glycoprotein frequently used in combination with IL-5 receptor alpha (IL5Rα) for the identification of murine eosinophils...
April 12, 2018: Journal of Leukocyte Biology
Petr Šteiner, Simon Andreasen, Petr Grossmann, Lukáš Hauer, Tomáš Vaněček, Markéta Miesbauerová, Thalita Santana, Katalin Kiss, David Slouka, Alena Skálová
Adenoid cystic carcinoma (AdCC) of the salivary glands is characterized by MYB-NFIB or MYBL1-NFIB fusion, prolonged but relentlessly progressive clinical course with frequent recurrences, and development of distant metastasis resulting in high long-term mortality. Currently, no effective therapy is available for patients with advanced non-resectable and/or metastatic disease. Complicating the clinical management of this patient group is the lack of prognostic markers. The purpose of this study is to investigate the prognostic value of 1p36 loss in patients with AdCC...
April 4, 2018: Virchows Archiv: An International Journal of Pathology
Yuan Ma, Sergio Gil, Klaus D Grasser, Paloma Mas
The rhythms of steady-state mRNA expression pervade nearly all circadian systems. However, the mechanisms behind the rhythmic transcriptional synthesis and its correlation with circadian expression remain fully unexplored, particularly in plants. Here, we discovered a multi-functional protein complex that orchestrates the rhythms of transcriptional activity in Arabidopsis thaliana. The expression of the circadian oscillator genes TOC1 (TIMING OF CAB EXPRESSION1/PSEUDO-RESPONSE REGULATOR1) and PRR5 (PSEUDO-RESPONSE REGULATOR5) initially relies on the modular function of the clock-related factor RVE8: its MYB domain provides the DNA binding specificity, while its LCL domain recruits the clock components, LNKs, to target promoters...
April 4, 2018: Plant Cell
Rihua Lei, Zhenbing Ma, Diqiu Yu
Male gametogenesis in plants is tightly controlled and involves the complex and precise regulation of transcriptional reprogramming. Interactions between WRKY proteins and VQ motif-containing proteins are required to control these complicated transcriptional networks. However, our understanding of the mechanisms by which these complexes affect downstream gene expression is quite limited. In this study, we found that WRKY2 and WKRY34 repress MYB97 , MYB101 , and MYB120 expression during male gametogenesis. MYB expression was up-regulated in the wrky2-1 wrky34-1 vq20-1 triple mutant during male gametogenesis...
2018: Frontiers in Plant Science
Manik Chahal, Erin Pleasance, Jasleen Grewal, Eric Zhao, Tony Ng, Erin Chapman, Martin R Jones, Yaoqing Shen, Karen L Mungall, Melika Bonakdar, Gregory A Taylor, Yussanne Ma, Andrew J Mungall, Richard A Moore, Howard Lim, Daniel Renouf, Stephen Yip, Steven J M Jones, Marco A Marra, Janessa Laskin
Metastatic adenoid cystic carcinomas (ACCs) can cause significant morbidity and mortality. Because of their slow growth and relative rarity, there is limited evidence for systemic therapy regimens. Recently, molecular profiling studies have begun to reveal the genetic landscape of these poorly understood cancers, and new treatment possibilities are beginning to emerge. The objective is to use whole-genome and transcriptome sequencing and analysis to better understand the genetic alterations underlying the pathology of metastatic and rare ACCs and determine potentially actionable therapeutic targets...
April 2018: Cold Spring Harbor Molecular Case Studies
Petr P Laktionov, Daniil A Maksimov, Stanislav E Romanov, Polina A Antoshina, Olga V Posukh, Helen White-Cooper, Dmitry E Koryakov, Stepan N Belyakin
BACKGROUND: During Drosophila spermatogenesis, testis-specific meiotic arrest complex (tMAC) and testis-specific TBP-associated factors (tTAF) contribute to activation of hundreds of genes required for meiosis and spermiogenesis. Intriguingly, tMAC is paralogous to the broadly expressed complex Myb-MuvB (MMB)/dREAM and Mip40 protein is shared by both complexes. tMAC acts as a gene activator in spermatocytes, while MMB/dREAM was shown to repress gene activity in many cell types. RESULTS: Our study addresses the intricate interplay between tMAC, tTAF, and MMB/dREAM during spermatogenesis...
April 2, 2018: Epigenetics & Chromatin
Muhammad Anwar, Guiqing Wang, Jiacheng Wu, Saquib Waheed, Andrew C Allan, Lihui Zeng
R2R3 MYB transcription factors play key functions in the regulation of secondary metabolites. In the present study, a R2R3 MYB transcriptional factor NtMYB2 was identified from Chinese narcissus ( Narcissus tazetta L. var. Chinensis Roem) and functionally characterized. NtMYB2 belongs to subgroup 4 of the R2R3 MYB transcription factor family that are related to repressor MYBs involved in the regulation of anthocyanin and flavonoids. Transient expression confirmed that NtMYB2 strongly reduced the red pigmentation induced by MYB- anthocyanin activators in agro-infiltrated tobacco leaves...
March 28, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Zexiong Chen, Guohua Liu, Ning Tang, Zhengguo Li
Lonicera macranthoides is an important medicinal plant widely used in traditional Chinese medicine. Luteoloside is a critical bioactive compound in L. macranthoides . To date, the molecular mechanisms underlying luteoloside biosynthesis are still largely unknown. In this work, high performance liquid chromatography (HPLC) was employed to determine the luteoloside contents in leaves, stems, and flowers at different developmental stages. Results showed that senescing leaves can accumulate large amounts of luteoloside, extremely higher than that in young and semi-lignified leaves and other tissues...
March 28, 2018: International Journal of Molecular Sciences
Rasmieh Hamid, Rukam S Tomar, Hassan Marashi, Saeid Malekzadeh Shafaroudi, Balaji A Golakiya, Motahhareh Mohsenpour
Cytoplasmic Male Sterility is maternally inherited trait in plants, characterized by failure to produce functional pollen during anther development. Anther development is modulated through the interaction of nuclear and mitochondrial genes. In the present study, differential gene expression of floral buds at the sporogenous stage (SS) and microsporocyte stage (MS) between CGMS and its fertile maintainer line of cotton plants was studied. A total of 320 significantly differentially expressed genes, including 20 down-regulated and 37 up-regulated in CGMS comparing with its maintainer line at the SS stage, as well as and 89 down-regulated and 4 up-regulated in CGMS compared to the fertile line at MS stage...
March 22, 2018: Gene
Xiaolan Jiang, Keyi Huang, Guangshun Zheng, Hua Hou, Peiqiang Wang, Han Jiang, Xuecheng Zhao, Mingzhuo Li, Shuxiang Zhang, Yajun Liu, Liping Gao, Lei Zhao, Tao Xia
Tea is one of the most widely consumed nonalcoholic beverages worldwide. Polyphenols are nutritional compounds present in the leaves of tea plants. Although numerous genes are functionally characterized to encode enzymes that catalyze the formation of diverse polyphenolic metabolites, transcriptional regulation of those different pathways such as late steps of the proanthcoyanidin (PA) pathway remains unclear. In this study, using different tea transcriptome databases, we screened at least 140 R2R3-MYB transcription factors (TFs) and grouped them according to the basic function domains of the R2R3 MYB TF superfamily...
May 2018: Plant Science: An International Journal of Experimental Plant Biology
Guoyun Zhang, Tong Zhang, Juanjuan Liu, Jianguo Zhang, Caiyun He
Atmospheric carbon dioxide (CO2 ) concentration increases every year. It is critical to understand the elevated CO2 response molecular mechanisms of plants using genomic techniques. Hippophae rhamnoides L. is a high stress resistance plant species widely distributed in Europe and Asia. However, the molecular mechanism of elevated CO2 response in H. rhamnoides has been limited. In this study, transcriptomic analysis of two sea buckthorn cultivars under different CO2 concentrations was performed, based on the next-generation illumina sequencing platform and de novo assembly...
March 21, 2018: Gene
Liubov Kyrpychova, Tomas Vanecek, Petr Grossmann, Petr Martinek, Petr Steiner, Ladislav Hadravsky, Irena E Belousova, Ksenya V Shelekhova, Marian Svajdler, Pavol Dubinsky, Michal Michal, Dmitry V Kazakov
Adenoid cystic carcinoma (ACC) of the skin is a rare malignant neoplasm histologically identical to homonymous tumors in other organs. Cutaneous ACC has been found to harbor MYB gene activations, either through MYB chromosomal abnormalities or by generation of the MYB-NFIB fusion. In salivary gland ACC, in addition to the MYB gene, alterations in MYBL1, the gene closely related to MYB, have been reported. We studied 10 cases of cutaneous ACC (6 women, 4 men; and age range 51-83 years) for alterations in the MYB, NFIB, and MYBL1 genes, using FISH and PCR...
March 21, 2018: American Journal of Dermatopathology
Won Tae Yang, Dongwon Baek, Dae-Jin Yun, Kwang Sik Lee, So Yeon Hong, Ki Deuk Bae, Young Soo Chung, Yong Sham Kwon, Du Hyun Kim, Ki Hong Jung, Doh Hoon Kim
Myeloblastosis (MYB) transcription factors play central roles in plant developmental processes and in responses to nutrient deficiency. In this study, OsMYB5P, an R2R3-MYB transcription factor, was isolated and identified from rice (Oryza sativa L. 'Dongjin') under inorganic phosphate (Pi)-deficient conditions. OsMYB5P protein is localized to the nucleus and functions as a transcription activator in plant development. Overexpression of OsMYB5P in rice and Arabidopsis (Arabidopsis thaliana Col-0) increases tolerance to phosphate starvation, whereas OsMYB5P knock-out through RNA interference increases sensitivity to Pi depletion in rice...
2018: PloS One
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