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Sen Yang, Yanling Cai, Xingwang Liu, Mingming Dong, Yaqi Zhang, Shuying Chen, Wenbo Zhang, Yujing Li, Min Tang, Xuling Zhai, Yiqun Weng, Huazhong Ren
The fruit epidermal features such as the number and size of trichomes or spines are important fruit quality traits for cucumber production. Little is known about the molecular mechanisms underlying fruit spine formation in cucumber. Here, we reported functional characterization of the cucumber CsMYB6 gene, which encodes a MIXTA-like MYB transcription factor that is an important player in regulating fruit trichome development in cucumber. Spatial-temporal expression analyses revealed high level expression of CsMYB6 in the epidermis of cucumber ovaries during fruit spine initiation, which was similar to CsTRY, a homolog of the Arabidopsis TRY gene that also plays a key role in trichome development...
February 8, 2018: Journal of Experimental Botany
Guo-Jun Geng, Ying-Tao Yang, Jie Jiang, Xiu-Yi Yu, Xian-En Fa
Non-small-cell lung cancer (NSCLC) is one of the leading causes of cancer mortality worldwide. A growing body of evidence indicates that microRNA (miR) have important and diverse roles in the proliferation, apoptosis and metastasis of human cancer cells. In the present study, the molecular regulation mechanism of miR-30a and its potential target, Myb-related protein B (MYBL2) was investigated in NSCLC. Reverse transcription-quantitative polymerase chain reaction results showed that miR-30a was significantly downregulated in NSCLC tissues compared with adjacent normal tissues (P<0...
February 2018: Experimental and Therapeutic Medicine
Kevin C Flanagan, Elise Alspach, Ermira Pazolli, Shankar Parajuli, Qihao Ren, Laura L Arthur, Roberto Tapia, Sheila A Stewart
Tumorigenesis results from the convergence of cell autonomous mutations and corresponding stromal changes that promote tumor cell growth. Senescent cells, which secrete a plethora of pro-tumorigenic factors termed the senescence-associated secretory phenotype (SASP), play an important role in tumor formation. Investigation into SASP regulation revealed that many but not all SASP factors are subject to NF-kB and p38MAPK regulation. However, many pro-tumorigenic SASP factors, including osteopontin (OPN), are not responsive to these canonical pathways leaving the regulation of these factors an open question...
January 2, 2018: Oncotarget
Ming Ma, Riyang Zhao, Xingxiao Yang, Lianmei Zhao, Lihua Liu, Cong Zhang, Xuexiao Wang, Baoen Shan
OBJECTIVES: Burkitt lymphoma is one of the most common types of haematopoietic malignancy in children and adolescents. Mda-7/IL-24 had been identified as a differentiation inducer of Burkitt lymphoma cells. Previous studies have revealed that knockdown of C-myb can also lead to the terminal differentiation of Burkitt lymphoma cells. The aim of the present study was to investigate the correlation between the expression of Mda-7/IL-24 and C-myb, as well as their prognostic significance, for Burkitt lymphoma patients...
February 8, 2018: Hematology (Amsterdam, Netherlands)
L F Schütz, R E Hurst, N B Schreiber, L J Spicer
We reported previously that fibroblast growth factor 9 (FGF9) acts as an antidifferentiation factor, stimulating proliferation of granulosa cells (GCs) and theca cells (TCs) while suppressing hormone-induced steroidogenesis of these cells. How FGF9 acts to simultaneously suppress steroidogenesis and stimulate proliferation remains to be fully elucidated. Thus, this study was undertaken to clarify the effects of FGF9 on the TC transcriptome. Ovaries were obtained from beef heifers at a local abattoir, TCs were isolated from large antral follicles, and cultured with or without 30 ng/mL of FGF9 for 24 h in the presence of LH and IGF-1...
January 4, 2018: Domestic Animal Endocrinology
Xue Liu, Dayong Li, Donglei Zhang, Dedong Yin, Yi Zhao, Chengjun Ji, Xianfeng Zhao, Xiaobing Li, Qun He, Runsheng Chen, Songnian Hu, Lihuang Zhu
Natural antisense long noncoding RNAs (lncRNAs) are widespread in many organisms. However, their biological functions remain largely unknown, particularly in plants. We report the identification and characterization of an endogenous lncRNA, TWISTED LEAF (TL), which is transcribed from the opposite strand of the R2R3 MYB transcription factor gene locus, OsMYB60, in rice (Oryza sativa). TL and OsMYB60 were found to be coexpressed in many different tissues, and the expression level of TL was higher than that of OsMYB60...
February 7, 2018: New Phytologist
Yuki Togashi, Akito Dobashi, Seiji Sakata, Yukiko Sato, Satoko Baba, Akira Seto, Hiroki Mitani, Kazuyoshi Kawabata, Kengo Takeuchi
MYB-NFIB and MYBL1-NFIB have been reported in ~60% of adenoid cystic carcinoma cases, but driver alterations in the remaining ~40% of adenoid cystic carcinoma remain unclear. We examined 100 adenoid cystic carcinoma cases for MYB and MYBL1 locus rearrangements by fluorescence in situ hybridization (FISH) with originally designed probe sets using formalin-fixed paraffin-embedded materials. Approximately one-third of samples were also analyzed by fusion transcript-specific RT-PCR and capture RNA sequencing. In the 27 cases with frozen materials, MYB-NFIB and MYBL1-NFIB fusion transcripts were detected in 9 (33%) and 6 cases (22%) by RT-PCR, respectively...
February 6, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
Masahiko Yanagita, Jason J Luke, Frank S Hodi, Pasi A Jänne, Cloud P Paweletz
Isolation of circulating tumor cells (CTCs) from blood of melanoma patients has been difficult owing to inconsistent expression of surface antigens. Here we report on the isolation, detection, and characterization of CTCs from blood of melanoma patients using microfiltration and fluorescent in-situ hybridization (FISH). Two tubes of blood from 15 patients with advanced melanoma were collected. These two tubes subsequently underwent filtration through a membrane with pore sizes of 7.5 μm. Isolated cells from one tube were analyzed by FISH for RREB1 (6p24), MYB (6q32), SE6 (D6Z1), and CCND1 (11q13) and the other paired specimen was analyzed by immunofluorescence for HMB45, melanoma-associated antigen recognized by T cells-1, tyrosinase and melanogenesis associated transcription factor...
February 5, 2018: Melanoma Research
Kaixuan Zhang, Maria D Logacheva, Yu Meng, Jianping Hu, Dongpu Wan, Long Li, Janovská Dagmar, Zhiyong Wang, Milen I Georgiev, Zhuo Yu, Fuyu Yang, Mingli Yan, Meiliang Zhou
Jasmonates (JAs) are plant hormones that induce many secondary metabolites accumulation, such as rutin in buckwheat, via regulation of JAs-responsive transcription factors (TFs). Here, we report on the identification of a clade of JAs-responsive subgroup 4 MYB TFs, FtMYB13, FtMYB14, FtMYB15 and FtMYB16, directly repressing rutin biosynthesis in Fagopyrum tataricum. Immunoblot analysis showed that FtMYB13, FtMYB14 and FtMYB15 could be degraded via 26S proteasome in COI1-dependent JA signaling pathway, and this degradation is due to the SID motif in their C-terminus...
January 31, 2018: Journal of Experimental Botany
Anna Coulibaly, Astrid Haas, Simone Steinmann, Anke Jakobs, Thomas J Schmidt, Karl-Heinz Klempnauer
Myb is a key regulator of hematopoietic progenitor cell proliferation and differentiation and has emerged as a potential target for the treatment of acute leukemia. Using a myeloid cell line with a stably integrated Myb-inducible reporter gene as a screening tool we have previously identified Celastrol, a natural compound with anti-tumor activity, as a potent Myb inhibitor that disrupts the interaction of Myb with the co-activator p300. We showed that Celastrol inhibits the proliferation of acute myeloid leukemia (AML) cells and prolongs the survival of mice in an in vivo model of AML, demonstrating that targeting Myb with a small-molecule inhibitor is feasible and might have potential as a therapeutic approach against AML...
2018: PloS One
Liang Shen, Ming Liu, Wei Liu, Jing Cui, Changzhong Li
In the present study, the RNA sequencing (RNA-seq) data of uterine corpus endometrial carcinoma (UCEC) samples were collected and analyzed using bioinformatics tools to identify potential genes associated with the development of UCEC. UCEC RNA-seq data were downloaded from The Cancer Genome Atlas database. Differential analysis was performed using edgeR software. A false discovery rate <0.01 and |log2(fold change)|>1 were set as the cut-off criteria to screen for differentially expressed genes (DEGs)...
January 2018: Oncology Letters
Xiaoming Sun, José Tomás Matus, Darren Chern Jan Wong, Zemin Wang, Fengmei Chai, Langlang Zhang, Ting Fang, Li Zhao, Yi Wang, Yuepeng Han, Qingfeng Wang, Shaohua Li, Zhenchang Liang, Haiping Xin
Grapevine (Vitis vinifera L.) is a widely cultivated fruit crop whose growth and productivity are largely affected by low temperatures. On the other hand, wild Vitis species represent valuable genetic resources of natural stress tolerance. We have isolated and characterized a MYB-like gene encoding a putative GARP-type transcription factor from Amur grape (V. amurensis) designated as VaAQUILO. AQUILO (AQ) is induced by cold in both V. amurensis and V. vinifera and its overexpression results in significantly improved tolerance to cold both in transgenic Arabidopsis and Amur grape calli...
January 29, 2018: Journal of Experimental Botany
H Earl Petzold, Bidisha Chanda, Chengsong Zhao, Stephen B Rigoulot, Eric P Beers, Amy M Brunner
DIVARICATA AND RADIALIS INTERACTING FACTOR (DRIF) from snapdragon (Antirrhinum majus) is a MYB/SANT protein that interacts with related MYB/SANT proteins, RADIALIS and DIVARICATA, through its N-terminal MYB/SANT domain. In addition to the MYB/SANT domain, DRIF contains a C-terminal Domain of Unknown Function (DUF3755). Here we describe novel protein-protein interactions involving a poplar (Populus trichocarpa) homolog of DRIF, PtrDRIF1. In addition to interacting with poplar homologs of RADIALIS (PtrRAD1) and DIVARICATA (PtrDIV4), PtrDRIF1 interacted with members of other families within the homeodomain-like superfamily, including PtrWOX13c, a WUSCHEL-RELATED HOMEOBOX protein, and PtrKNAT7, a KNOTTED1-LIKE HOMEOBOX protein...
January 31, 2018: Plant Journal: for Cell and Molecular Biology
Karim L Kreft, Gijsbert P Van Nierop, Sandra M J Scherbeijn, Malou Janssen, Georges M G M Verjans, Rogier Q Hintzen
Objective: To assess whether MS genetic risk polymorphisms (single nucleotide polymorphism [SNP]) contribute to the enhanced humoral immune response against Epstein-Barr virus (EBV) infection in patients with MS. Methods: Serum anti-EBV nuclear antigen 1 (EBNA-1) and early antigen D (EA-D) immunoglobulin γ (IgG) levels were quantitatively determined in 668 genotyped patients with MS and 147 healthy controls. Anti-varicella-zoster virus (VZV) IgG levels were used as a highly prevalent, non-MS-associated control herpesvirus...
November 2017: Neurology® Neuroimmunology & Neuroinflammation
Nan Li, Han Wu, Qiangqiang Ding, Huihui Li, Zhifei Li, Jing Ding, Yi Li
Anthocyanins are naturally occurring secondary metabolites, responsible for the color of many plants. The Arabidopsis thaliana MYB90/PAP2 (production of anthocyanin pigment 2) was introduced into tomato to study its effect on anthocyanin accumulation. The transgenic tomato displayed much greater anthocyanin accumulation than wild type in all plant organs, but the organs were not fully purple in color except for the stamen. The expression of anthocyanin biosynthetic genes and an anthocyanin-related basic helix-loop-helix (bHLH) gene SlAN1 was significantly increased in the transgenic line, suggesting that ectopic expression of AtPAP2 increases the expression of anthocyanin-related structural and regulatory genes to enhance anthocyanin content...
January 26, 2018: Functional & Integrative Genomics
Jemima Brinton, James Simmonds, Cristobal Uauy
BACKGROUND: There is an urgent need to increase global crop production. Identifying and combining specific genes controlling distinct biological processes holds the potential to enhance crop yields. Transcriptomics is a powerful tool to gain insights into the complex gene regulatory networks that underlie such traits, but relies on the availability of a high-quality reference sequence and accurate gene models. Previously, we identified a grain weight QTL on wheat chromosome 5A (5A QTL) which acts during early grain development to increase grain length through cell expansion in the pericarp...
January 25, 2018: BMC Plant Biology
Marek D Koter, Magdalena Święcicka, Mateusz Matuszkiewicz, Andrzej Pacak, Natalia Derebecka, Marcin Filipecki
Cyst-forming plant-parasitic nematodes are pests threatening many crops. By means of their secretions cyst nematodes induce the developmental and metabolic reprogramming of host cells that lead to the formation of a syncytium, which is the sole food source for growing nematodes. The in depth micro RNA (miRNA) dynamics in the syncytia induced by Globodera rostochiensis in tomato roots was studied. The miRNAomes were obtained from syncytia covering the early and intermediate developmental stages, and were the subject of differential expression analysis...
March 2018: Plant Science: An International Journal of Experimental Plant Biology
Da-Hye Kim, Sangkyu Park, Jong-Yeol Lee, Sun-Hwa Ha, Sun-Hyung Lim
Flower color is a main target for flower breeding. A transgenic approach for flower color modification requires a transgene and a flower-specific promoter. Here, we expressed the B-peru gene encoding a basic helix loop helix (bHLH) transcription factor (TF) together with the mPAP1 gene encoding an R2R3 MYB TF to enhance flower color in tobacco (Nicotiana tabacum L.), using the tobacco anthocyanidin synthase (ANS) promoter (PANS) to drive flower-specific expression. The transgenic tobacco plants grew normally and produced either dark pink (PANSBP_DP) or dark red (PANSBP_DR) flowers...
January 20, 2018: International Journal of Molecular Sciences
Xingming Sun, Zhanying Zhang, Chao Chen, Wei Wu, Nannan Ren, Conghui Jiang, Jianping Yu, Yan Zhao, Xiaoming Zheng, Qingwen Yang, Hongliang Zhang, Jinjie Li, Zichao Li
Floral organs in rice (Oryza sativa) can be purple, brown or red in color due to the accumulation of flavonoids. But the molecular mechanism underlying specific organ pigmentation is not clear. Here, we propose a C-S-A gene model for rice hull pigmentation and characterize through genetic, molecular and metabolomic approaches. Further we conduct phylogenetic studies to reveal the evolution of rice color. In this gene system, C1 encodes a R2R3-MYB transcription factor and acts as a color-producing gene and S1 encodes a bHLH protein that functions in a tissue-specific manner...
January 18, 2018: Journal of Experimental Botany
Fang Liu, Yuanjun Yang, Jianwei Gao, Changle Ma, Yuping Bi
In this study, a red mutant was obtained through in vitro regeneration of a wild purple potato. High-performance liquid chromatography and Mass spectrometry analysis revealed that pelargonidin-3-O-glucoside and petunidin-3-O-glucoside were main anthocyanins in the mutant and wild type tubers, respectively. In order to thoroughly understand the mechanism of anthocyanin transformation in two materials, a comparative transcriptome analysis of the mutant and wild type was carried out through high-throughput RNA sequencing, and 295 differentially expressed genes (DEGs) were obtained...
2018: PloS One
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