keyword
https://read.qxmd.com/read/21410405/stat3-controls-matrix-metalloproteinase-1-expression-in-colon-carcinoma-cells-by-both-direct-and-ap-1-mediated-interaction-with-the-mmp-1-promoter
#21
JOURNAL ARTICLE
Constance Zugowski, Franziska Lieder, Annekatrin Müller, Josephine Gasch, Florian M Corvinus, Richard Moriggl, Karlheinz Friedrich
Aberrant activation of STAT3 in colorectal carcinoma (CRC) tissue is correlated with elevated expression of matrix metalloproteinase-1 (MMP-1). We analyzed transcriptional regulation of the human MMP-1 promoter in CRC cells by tyrosine phosphorylated (pY-) STAT3. One of six putative STAT binding elements within a 4.3 kb MMP-1 trancriptional promoter fragment showed a particular high affinity for STAT3 in vitro. However, the most profound regulatory influence on MMP-1 promoter activity resides in a proximal region relative to the transcriptional start, bearing a pair of putative binding sites for STAT3 and AP-1...
May 2011: Biological Chemistry
https://read.qxmd.com/read/21316751/dual-promoter-lentiviral-vector-generates-transgenic-mice-expressing-e2-csfv-glycoprotein-in-their-milk-but-impairs-early-identification-of-transgenic-embryos
#22
JOURNAL ARTICLE
Oliberto Sánchez Ramos, Yanet Prieto Carratalá, Silvia Gómez Puerta, Natalie C Parra Pereira, Lester Suárez Amarán, Silvana P Jimenez Chaves, Jorge R Toledo Alonso
Lentiviral vectors containing the green fluorescent protein gene have been successfully used to select transgenic embryos before transfer to a surrogate mother. However, there are apparently no reports regarding early detection of transgenic embryos using a lentiviral vector carrying an additional transcription unit for tissue-specific expression of a valuable protein. In this study, two HIV-based lentiviral vectors were constructed. The first one contained the green fluorescent protein (GFP) coding sequence driven by the early SV40 promoter (Lv-G), whereas the other contained an additional transcription unit for the expression of E2 glycoprotein from classical swine fever virus, driven by a 1...
April 15, 2011: Theriogenology
https://read.qxmd.com/read/21162712/characterization-of-a-novel-jnk-c-jun-n-terminal-kinase-inhibitory-peptide
#23
JOURNAL ARTICLE
Kevin R W Ngoei, Bruno Catimel, Nicole Church, Daisy S Lio, Con Dogovski, Matthew A Perugini, Paul M Watt, Heung-Chin Cheng, Dominic C H Ng, Marie A Bogoyevitch
An improved understanding of the roles of protein kinases in intracellular signalling and disease progression has driven significant advances in protein kinase inhibitor discovery. Peptide inhibitors that target the kinase protein substrate-binding site have continued to attract attention. In the present paper, we describe a novel JNK (c-Jun N-terminal kinase) inhibitory peptide PYC71N, which inhibits JNK activity in vitro towards a range of recombinant protein substrates including the transcription factors c-Jun, ATF2 (activating trancription factor 2) and Elk1, and the microtubule regulatory protein DCX (doublecortin)...
March 15, 2011: Biochemical Journal
https://read.qxmd.com/read/20833631/mitogenesisdb-an-expression-data-mining-tool-to-explore-spatio-temporal-dynamics-of-mitochondrial-biogenesis
#24
JOURNAL ARTICLE
Jean-Christophe Gelly, Mickael Orgeur, Claude Jacq, Gaëlle Lelandais
Mitochondria constitute complex and flexible cellular entities, which play crucial roles in normal and pathological cell conditions. The database MitoGenesisDB focuses on the dynamic of mitochondrial protein formation through global mRNA analyses. Three main parameters confer a global view of mitochondrial biogenesis: (i) time-course of mRNA production in highly synchronized yeast cell cultures, (ii) microarray analyses of mRNA localization that define translation sites and (iii) mRNA transcription rate and stability which characterize genes that are more dependent on post-transcriptional regulation processes...
January 2011: Nucleic Acids Research
https://read.qxmd.com/read/20798532/reference-profile-correlation-reveals-estrogen-like-trancriptional-activity-of-curcumin
#25
JOURNAL ARTICLE
Beatrice E Bachmeier, Valentina Mirisola, Francesco Romeo, Luca Generoso, Alessia Esposito, Raffaella Dell'eva, Fabiola Blengio, Peter H Killian, Adriana Albini, Ulrich Pfeffer
BACKGROUND: Several secondary metabolites from herbal nutrient products act as weak estrogens (phytoestrogens), competing with endogenous estrogen for binding to the estrogen receptors and inhibiting steroid converting enzymes. However, it is still unclear whether these compounds elicit estrogen dependent transcription of genes at physiological concentrations. METHODS: We compare the effects of physiological concentrations (100 nM) of the two phytoestrogens Enterolactone and Quercetin and the suspected phytoestrogen Curcumin on gene expression in the breast cancer cell line MCF7 with the effects elicited by 17-beta-estradiol (E2)...
2010: Cellular Physiology and Biochemistry
https://read.qxmd.com/read/20448844/effect-of-diet-supplementation-on-the-expression-of-bovine-genes-associated-with-fatty-acid-synthesis-and-metabolism
#26
JOURNAL ARTICLE
Sandeep J Joseph, Kelly R Robbins, Enrique Pavan, Scott L Pratt, Susan K Duckett, Romdhane Rekaya
Conjugated linoleic acids (CLA) are of important nutritional and health benefit to human. Food products of animal origin are their major dietary source and their concentration increases with high concentrate diets fed to animals. To examine the effects of diet supplementation on the expression of genes related to lipid metabolism, 28 Angus steers were fed either pasture only, pasture with soybean hulls and corn oil, pasture with corn grain, or high concentrate diet. At slaughter, samples of subcutaneous adipose tissue were collected, from which RNA was extracted...
March 31, 2010: Bioinformatics and Biology Insights
https://read.qxmd.com/read/19540338/jnk-protects-drosophila-from-oxidative-stress-by-trancriptionally-activating-autophagy
#27
JOURNAL ARTICLE
Hai Wu, Meng C Wang, Dirk Bohmann
JNK signaling functions to induce defense mechanisms that protect organisms against acute oxidative and xenobiotic insults. Using Drosophila as a model system, we investigated the role of autophagy as such a JNK-regulated protective mechanism. We show that oxidative stress can induce autophagy in the intestinal epithelium by a mechanism that requires JNK signaling. Consistently, artificial activation of JNK in the gut gives rise to an autophagy phenotype. JNK signaling can induce the expression of several autophagy-related (ATG) genes, and the integrity of these genes is required for the stress protective function of the JNK pathway...
August 2009: Mechanisms of Development
https://read.qxmd.com/read/18852534/retinoic-acid-receptor-beta2-re-expression-and-growth-inhibition-in-thyroid-carcinoma-cell-lines-after-5-aza-2-deoxycytidine-treatment
#28
JOURNAL ARTICLE
F Y Miasaki, A Vivaldi, R Ciampi, L Agate, P Collecchi, A Capodanno, A Pinchera, R Elisei
The treatment of both undifferentiated and de-differentiated thyroid tumors, which are unresponsive to radioiodine, represents one of the biggest challenges for thyroidologists. The aim of the present study was to investigate in vitro the methylation status of retinoic acid receptors (RAR)beta2 promoter and the effect of the demethylating agent 5-aza-2'-deoxycytidine (5-Aza-CdR) on 5 human thyroid cancer cell lines. The methylation status of RARbeta2 promoter was analyzed by methylation-specific PCR. The effect of 5-Aza-CdR on cell growth and apoptosis was evaluated by cell counting, enzymelinked immunosorbent assay tests and fluorescence-activated cell sorting analysis, while the effect on the expression of RAR and thyroid-specific genes was measured by qualitative and quantitative RT-PCR...
August 2008: Journal of Endocrinological Investigation
https://read.qxmd.com/read/17983269/the-transcription-factor-mrr1p-controls-expression-of-the-mdr1-efflux-pump-and-mediates-multidrug-resistance-in-candida-albicans
#29
JOURNAL ARTICLE
Joachim Morschhäuser, Katherine S Barker, Teresa T Liu, Julia BlaB-Warmuth, Ramin Homayouni, P David Rogers
Constitutive overexpression of the MDR1 (multidrug resistance) gene, which encodes a multidrug efflux pump of the major facilitator superfamily, is a frequent cause of resistance to fluconazole and other toxic compounds in clinical Candida albicans strains, but the mechanism of MDR1 upregulation has not been resolved. By genome-wide gene expression analysis we have identified a zinc cluster transcription factor, designated as MRR1 (multidrug resistance regulator), that was coordinately upregulated with MDR1 in drug-resistant, clinical C...
November 2007: PLoS Pathogens
https://read.qxmd.com/read/16634641/tyrosine-phosphorylation-is-required-for-functional-activation-of-disulfide-containing-constitutively-active-stat-mutants
#30
JOURNAL ARTICLE
Forrester J Liddle, James V Alvarez, Valeria Poli, David A Frank
Aberrant activation of STAT transcription factors has been implicated in a variety of cancers. Constitutively active forms of STAT1 and STAT3 (STAT1C and STAT3C) have been developed to determine the effects of STAT activation in isolation from other cytokine-stimulated signaling pathways. These mutants were created by engineering cysteine residues into the carboxy terminus of each STAT molecule, allowing a hypothesized disulfide bond to form between two unphosphorylated monomers. To determine whether the presence of cysteine residues is sufficient to allow for functional activation in the absence of tyrosine phosphorylation, we developed STAT1C and STAT3C mutants that are unable to be phosphorylated on the critical tyrosine residue...
May 2, 2006: Biochemistry
https://read.qxmd.com/read/16543149/the-molecular-basis-of-vitamin-d-receptor-and-beta-catenin-crossregulation
#31
JOURNAL ARTICLE
Salimuddin Shah, Md Naimul Islam, Sivanesan Dakshanamurthy, Imran Rizvi, Mahadev Rao, Roger Herrell, Glendon Zinser, Meggan Valrance, Ana Aranda, Dino Moras, Anthony Norman, JoEllen Welsh, Stephen W Byers
The signaling/oncogenic activity of beta-catenin can be repressed by activation of the vitamin D receptor (VDR). Conversely, high levels of beta-catenin can potentiate the transcriptional activity of 1,25-dihydroxyvitamin D3 (1,25D). We show here that the effects of beta-catenin on VDR activity are due to interaction between the activator function-2 (AF-2) domain of the VDR and C terminus of beta-catenin. Acetylation of the beta-catenin C terminus differentially regulates its ability to activate TCF or VDR-regulated promoters...
March 17, 2006: Molecular Cell
https://read.qxmd.com/read/16475975/estrogen-receptor-alpha-impact-of-ligands-on-intracellular-shuttling-and-turnover-rate-in-breast-cancer-cells
#32
REVIEW
Guy Leclercq, Marc Lacroix, Ioanna Laïos, Guy Laurent
Estrogen receptors (alpha and beta) are members of the steroid/thyroid nuclear receptors superfamily of ligand-dependent transcription factors. Impact of the alpha isoform of estrogen receptor (ER) on breast cancer etiology and progression is now well established. Current therapeutic strategy to treat ER-positive breast cancer relies on the blockade of ER trancriptional activity by antiestrogens. Data accumulated during the last five years on the mechanism of action of ER enable one to foresee new strategies...
February 2006: Current Cancer Drug Targets
https://read.qxmd.com/read/16268485/cpg-methylation-of-the-pai-1-gene-5-flanking-region-is-inversely-correlated-with-pai-1-mrna-levels-in-human-cell-lines
#33
COMPARATIVE STUDY
Shan Gao, Sune Skeldal, Annelise Krogdahl, Jens Ahm Sørensen, Peter A Andreasen
The physiological and pathophysiological functions of PAI-1 are related to its expression by specific cell types in normal and diseased tissues. We analysed the contribution of DNA methylation to the variation in PAI-1 mRNA levels in five cell lines. We found varying frequencies of methylation of 25 CpGs in the -805/+152 region of the PAI-1 gene in Bowes, MCF-7 and U937 cells, while little or no methylation was detected in Hep2 and HT- 1080 cells. The methylation frequency was inversely correlated with PAI-1 mRNA level within its 20-fold range in Bowes, MCF-7, U937,and Hep2 cells, while the lack of methylation in both Hep2 and HT-1080 cells suggested another mechanism behind the 150-fold higher level in HT- 1080 cells than in Hep2 cells...
September 2005: Thrombosis and Haemostasis
https://read.qxmd.com/read/16093660/happy-together-the-life-and-times-of-ty-retrotransposons-and-their-hosts
#34
REVIEW
P Lesage, A L Todeschini
The aim of this review is to describe the level of intimacy between Ty retrotransposons (Ty1-Ty5) and their host the yeast Saccharomyces cerevisiae. The effects of Ty location in the genome and of host proteins on the expression and mobility of Ty elements are highlighted. After a brief overview of Ty diversity and evolution, we describe the factors that dictate Ty target-site preference and the impact of targeting on Ty and adjacent gene expression. Studies on Ty3 and Ty5 have been especially informative in unraveling the role of host factors (Pol III machinery and silencing proteins, respectively) and integrase in controlling the specificity of integration...
2005: Cytogenetic and Genome Research
https://read.qxmd.com/read/15347763/complex-regulation-of-the-synthesis-of-the-compatible-solute-ectoine-in-the-halophilic-bacterium-chromohalobacter-salexigens-dsm-3043t
#35
JOURNAL ARTICLE
M Isabel Calderón, Carmen Vargas, Fernando Rojo, Fernando Iglesias-Guerra, Laszlo N Csonka, Antonio Ventosa, Joaquín J Nieto
The synthesis of the compatible solute ectoine, mediated by the ectABC gene products, is the main mechanism used by the halophilic bacterium Chromohalobacter salexigens to cope with osmotic stress. Evidence was found that this process is regulated at the transcriptional level. S1 protection analyses performed with RNA extracted from cells grown in minimal medium at low (0.75 M NaCl) or high (2.5 M NaCl) osmolarity suggested the existence of four promoters upstream of ectA. Two of these (PectA1 and PectA2) might be recognized by the main vegetative sigma factor sigma(70), and one (PectA3) might be dependent on the general stress sigma factor sigma(S)...
September 2004: Microbiology
https://read.qxmd.com/read/15282747/targeted-insertion-of-an-ires-cre-into-the-hnf4alpha-locus-cre-mediated-recombination-in-the-liver-kidney-and-gut-epithelium
#36
COMPARATIVE STUDY
Stéphane D Vincent, Elizabeth J Robertson
The Hnf4alpha gene belongs to a family of trancriptional regulators required for liver development and function. Hnf4alpha is also expressed in other tissues, including the newly formed visceral endoderm of the early postimplantation embryo, and later in embryogenesis in the gut epithelium and the kidney. The regulatory sequences involved in controlling expression of Hnf4alpha at these diverse sites are not clearly understood. Here we used homologous recombination to introduce Cre recombinase coding sequences into the endogenous Hnf4alpha locus...
July 2004: Genesis: the Journal of Genetics and Development
https://read.qxmd.com/read/15056800/bromobenzene-induced-hepatotoxicity-at-the-transcriptome-level
#37
JOURNAL ARTICLE
Wilbert H M Heijne, Angela L Slitt, Peter J van Bladeren, John P Groten, Curtis D Klaassen, Rob H Stierum, Ben van Ommen
Rats were exposed to three levels of bromobenzene, sampled at 6, 24, and 48 h, and liver gene expression profiles were determined to identify dose and time-related changes. Expression of many genes changed transiently, and dependent on the dose. Few changes were identified after 6 h, but many genes were differentially expressed after 24 h, while after 48 h, only the high dose elicited large effects. Differentially expressed genes were involved in drug metabolism (upregulated GSTs, mEH, NQO1, Mrps, downregulated CYPs, sulfotransferases), oxidative stress (induced HO-1, peroxiredoxin, ferritin), GSH depletion (induced GCS-l, GSTA, GSTM) the acute phase response, and in processes like cholesterol, fatty acid and protein metabolism, and intracellular signaling...
June 2004: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://read.qxmd.com/read/14522138/characterization-of-a-spontaneously-transformed-chicken-mononuclear-cell-line
#38
JOURNAL ARTICLE
N C Rath, M S Parcells, H Xie, E Santin
We describe the characterization of a spontaneously transformed chicken monocytic cell line that developed as a single colony of cells in a heterophil culture that was inadvertently left in the incubator over a period of 25 days. These cells, hitherto named HTC, grow efficiently at both 37 or 41 degrees C in culture medium containing either 5% FBS or 2% chicken serum. The HTC cells are acid phosphatase positive, show expressions of both class I and class II major histocompatibility complex (MHC), CD44, K1, and K55 cell surface antigens, and engulf latex beads, produce nitrite and interleukin-6 on stimulation with bacterial lipopolysaccharide (LPS)...
November 15, 2003: Veterinary Immunology and Immunopathology
https://read.qxmd.com/read/12751796/regulation-of-gene-transcription-by-a-constitutively-active-mutant-of-activating-transcription-factor-2-atf2
#39
JOURNAL ARTICLE
Lars Steinmüller, Gerald Thiel
Activating transcription factor 2 (ATF2) belongs to the family of basic region leucine zipper (bZIP) proteins that are characterized by the presence of a basic domain that functions as the DNA-binding domain and a leucine zipper domain that is required for dimerization. Together with bZIP proteins of the Fos and Jun families, ATF2 constitutes the AP-1 transcription factor complex. The biological activity of ATF2 is controlled by phosphorylation of two threonine residues within the N-terminal activation domain...
April 2003: Biological Chemistry
https://read.qxmd.com/read/12234674/foxd4a-and-foxd4b-two-new-winged-helix-transcription-factors-are-expressed-in-human-leukemia-cell-lines
#40
JOURNAL ARTICLE
Bettina S Freyaldenhoven, Cora Fried, Klaus Wielckens
Winged helix factors are important regulators of embryonal development and tissue differentiation. They are also involved in translocations found in acute leukemias and solid tumors. We have detected transcripts from five known and four novel winged helix genes in leukemia cell lines and CD34(+) blood progenitor cells by reverse trancription-polymerase chain reaction with degenerate primers on the highly conserved DNA binding domain. The genomic clones coding for two new winged helix proteins, FOXD4a and FOXD4b were isolated by high-stringency hybridization of a human phage library...
July 10, 2002: Gene
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