Wanzhen Zhuang, Chengxiu Liu, Yilin Hong, Yue Zheng, Minjian Huang, Haijun Tang, Lilan Zhao, Zhixin Huang, Mingshu Tu, Lili Yu, Jianlin Chen, Yi Zhang, Xiongfeng Chen, Fan Lin, Qi Gao, Chundong Yu, Yi Huang
BACKGROUND: Aberrant fucosylation observed in cancer cells contributes to an augmented release of fucosylated exosomes into the bloodstream, where miRNAs including miR-4732-3p hold promise as potential tumor biomarkers in our pilot study. However, the mechanisms underlying the sorting of miR-4732-3p into fucosylated exosomes during lung cancer progression remain poorly understood. METHODS: A fucose-captured strategy based on lentil lectin-magnetic beads was utilized to isolate fucosylated exosomes and evaluate the efficiency for capturing tumor-derived exosomes using nanoparticle tracking analysis (NTA)...
April 23, 2024: Journal of Experimental & Clinical Cancer Research: CR