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Cell Cycle Arrest Biomarkers

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https://www.readbyqxmd.com/read/28335578/exploring-the-antitumor-mechanism-of-high-dose-cytarabine-through-the-metabolic-perturbations-of-ribonucleotide-and-deoxyribonucleotide-in-human-promyelocytic-leukemia-hl-60-cells
#1
Zheng Li, Jian-Ru Guo, Qian-Qian Chen, Cai-Yun Wang, Wei-Jia Zhang, Mei-Cun Yao, Wei Zhang
Despite the apparent clinical benefits of high-dose cytarabine (Ara-C) over lower dose Ara-C in acute myeloid leukemia (AML) therapy, the mechanism behind high-dose Ara-C therapy remains uncertain. In this study, a LC-MS-based method was carried out to investigate the metabolic alteration of ribonucleotide and deoxyribonucleotide in human promyelocytic leukemia cells (HL-60) after treatment with Ara-C to reveal its antitumor mechanism. The metabolic results revealed that four nucleotides (ATP, ADP, CDP, and dCTP) could be used as potential biomarkers indicating the benefit of high-dose Ara-C over lower dose Ara-C treatment...
March 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28318081/silencing-of-atf2-inhibits-growth-of-pancreatic-cancer-cells-and-enhances-sensitivity-to-chemotherapy
#2
Mu Li, Xingda Wu, Ning Liu, Xiaoying Li, Fanbin Meng, Shaowei Song
Pancreatic cancer is one of the leading causes of cancer-related death worldwide. Activating transcription factor 2 (ATF2) is a multifunctional transcription factor, and is implicated in tumor progress, yet its role in pancreatic cancer remains unclear. In the present study, the level of ATF2 in pancreatic cancer tissues and the adjacent non-tumorous tissues was detected by quantitative real-time PCR and western blot. The roles of ATF2 in the proliferation, cell cycle, and apoptosis of pancreatic cancer cells were investigated through ATF2 silencing, and the effect of ATF2 shRNA on the sensitivity of pancreatic cancer cells to gemcitabine, an anti-tumor drug, was explored...
March 20, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28275865/phosphorylase-kinase-%C3%AE-affects-colorectal-cancer-cell-growth-and-represents-a-novel-prognostic-biomarker
#3
Guanghui Wang, Wenbin Shen, Chen-Ying Liu, Yun Liu, Tingyu Wu, Ximao Cui, Tong Yu, Yilian Zhu, Jinglue Song, Peng Du, Long Cui
PURPOSE: To study the expression and intracellular localization of phosphorylase kinase β (PHKβ) protein in colorectal cancers (CRCs), analyze its correlation with clinicopathological features and prognosis, and study the biological roles and mechanism-of-action of PHKβ in CRC cell lines. METHODS: Quantitative polymerase chain reaction (qPCR) and western blot assays were performed to compare the expressions of PHKβ mRNA and protein in CRC tissues and matched normal mucosa...
March 8, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28261346/the-role-of-golph3l-in-the-prognosis-and-nact-response-in-cervical-cancer
#4
Yanling Feng, Fan He, Shumei Yan, He Huang, Qidan Huang, Ting Deng, Huini Wu, Bei Gao, Jihong Liu
Background: We previously reported GOLPH3L is a novel oncogene associated with ovarian cancer. The role of GOLPH3L in cervical cancer and its cellular functions has not been determined. This study investigated clinical significance of GOLPH3L and potential proteins and pathways associated with GOLPH3L in cervical squamous cell carcinoma. Methods: Immunohistochemistry and western blot were used to examine the expression of GOLPH3L in cervical squamous cell carcinoma tissue specimens and adjacent non-cancerous tissues...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28260104/mir-92a-promotes-tumor-growth-of-osteosarcoma-by-targeting-pten-akt-signaling-pathway
#5
Jie Xiao, Weifeng Yu, Kongzu Hu, Maoqiang Li, Jianwei Chen, Zhanchun Li
MicroRNAs (miRNAs) play critical roles in human cancers including osteosarcoma (OS). miR-92a has been found to be a cancer-related miRNA in many cancer types and it is upregulated in OS cell lines. However, the expression and biological function of miR-92a in OS have not been investigated. In this study, we showed that miR-92a expression was increased in OS tissues, and its high expression was correlated with clinical stage, T classification and histological differentiation. Furthermore, patients with high expression of miR-92a had a significantly poorer survival rate...
March 2, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28260063/adam9-functions-as-a-promoter-of-gastric-cancer-growth-which-is-negatively-and-post-transcriptionally-regulated-by-mir-126
#6
Junqing Wang, Yunyun Zhou, Xiaochun Fei, Xuehua Chen, Jiqi Yan, Bingya Liu, Zhenggang Zhu
A disintegrin and metalloproteinase domain 9 (ADAM9) is a membrane-anchored protein implicated in cell-cell and cell-matrix interactions, including the process of tumorigenesis. However, the role of ADAM9 in gastric cancer (GC) has not been clearly illustrated. In the present study, we found aberrant overexpression of ADAM9 in both GC tissues and cell lines. The expression of ADAM9 was significantly correlated with patient clinicopathological features including tumor size, local invasion, lymph node metastasis and tumor‑node‑metastasis (TNM) stage...
February 16, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28257844/ube2s-associated-with-oscc-proliferation-by-promotion-of-p21-degradation-via-the-ubiquitin-proteasome-system
#7
Shusaku Yoshimura, Atsushi Kasamatsu, Dai Nakashima, Manabu Iyoda, Hiroki Kasama, Tomoaki Saito, Toshikazu Takahara, Yosuke Endo-Sakamoto, Masashi Shiiba, Hideki Tanzawa, Katsuhiro Uzawa
Ubiquitin-conjugating enzyme E2S (UBE2S), a family of E2 protein in the ubiquitin-proteasome system, is highly expressed in several types of cancers; however, its roles in oral squamous cell carcinoma (OSCC) have not yet been well elucidated. The purpose of this study was to clarify the functional activities of UBE2S in OSCCs. We analyzed the expression levels of UBE2S in nine OSCC cell lines and primary OSCC tissues by quantitative reverse transcriptase-polymerase chain reaction, Western blotting, and immunohistochemistry (IHC)...
April 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28255639/cisplatin-nephrotoxicity-is-not-detected-by-urinary-cell-cycle-arrest-biomarkers-in-lung-cancer-patients
#8
Zeki Toprak, Egemen Cebeci, Serife Aysen Helvaci, Ilkim Deniz Toprak, Yasin Kutlu, Abdullah Sakin, Tufan Tukek
PURPOSE: Cisplatin is a chemotherapeutic agent with potential nephrotoxicity. Delayed increase in serum creatinine after cisplatin administration shows that serum creatinine may not be a sufficient marker for early detection of nephrotoxicity. Urinary insulin-like growth factor binding protein-7 (IGFBP7) and tissue inhibitor of metalloproteinases-2 (TIMP-2) which are cell-cycle arrest biomarkers have been proposed recently for early detection of acute kidney injury (AKI). Herein, we evaluated urinary TIMP-2/IGFBP7 levels before and after cisplatin administration to patients with lung cancer and their role was examined in the early diagnosis of AKI...
March 2, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28222070/inhibition-of-polypyrimidine-tract-binding-protein-3-induces-apoptosis-and-cell-cycle-arrest-and-enhances-the-cytotoxicity-of-5-fluorouracil-in-gastric-cancer-cells
#9
Xin Liang, Haiyang Shi, Liyan Yang, Cen Qiu, Shengchao Lin, Yingxue Qi, Jiyu Li, Aiguang Zhao, Jianwen Liu
BACKGROUND: Human polypyrimidine tract binding protein 3 (PTBP3) was first discovered in 1999 and has been well characterised as a differentiation regulator. However, its role in human cancer has rarely been reported. Our previous study revealed increased PTBP3 protein level in gastric cancer tissues. Downregulation of PTBP3 suppressed the proliferation and differentiation of gastric cancer cells in vivo. METHODS: PTBP3 mRNA levels in human gastric cancer and adjuvant non-tumour tissues were detected...
February 21, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28212559/the-selective-mek1-inhibitor-selumetinib-enhances-the-antitumor-activity-of-everolimus-against-renal-cell-carcinoma-in-vitro-and-in-vivo
#10
Yun Zou, Jianfeng Wang, Xuejiao Leng, Jiwei Huang, Wei Xue, Jin Zhang, Yiran Huang
Renal cell carcinoma (RCC) is a urologic malignant cancer and often diagnosed at an advanced stage, which results in high mortality. Targeted therapy may improve the quality of life and survival of patients who are not suitable for nephrectomy. Everolimus, an mTOR inhibitor, is currently used as sequential or second-line therapy for RCC refractory to Sunitinib or sorafenib. However, its efficiency is palliative. In this study, we evaluated whether the antitumor activity of everolimus against RCC is enhanced by Selumetinib, a selective MEK1 inhibitor...
February 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28212429/the-p53-isoform-delta133p53%C3%A3-regulates-cancer-cell-apoptosis-in-a-rhob-dependent-manner
#11
Nikola Arsic, Alexandre Ho-Pun-Cheung, Crapez Evelyne, Eric Assenat, Marta Jarlier, Christelle Anguille, Manon Colard, Mikaël Pezet, Pierre Roux, Gilles Gadea
The TP53 gene plays essential roles in cancer. Conventionally, wild type (WT) p53 is thought to prevent cancer development and metastasis formation, while mutant p53 has transforming abilities. However, clinical studies failed to establish p53 mutation status as an unequivocal predictive or prognostic factor of cancer progression. The recent discovery of p53 isoforms that can differentially regulate cell cycle arrest and apoptosis suggests that their expression, rather than p53 mutations, could be a more clinically relevant biomarker in patients with cancer...
2017: PloS One
https://www.readbyqxmd.com/read/28210763/artemisinin-and-its-derivatives-in-cancer-therapy-status-of-progress-mechanism-of-action-and-future-perspectives
#12
REVIEW
Archana Bhaw-Luximon, Dhanjay Jhurry
Since the late 1990s, there has been rapid multiplication of data on the anti-cancer properties of artemisinins. This article reviews the status of progress of artemisinin and its derivatives as anti-cancer agents in clinical trials, case reports, and in vitro/in vivo studies. Particular attention is laid on the combinations of artemisinins and synthetic chemodrugs to enhance the latter's efficacy. An attempt is here made to rationalize the synergistic effects of a few common anti-cancer drugs of the anthracycline, taxane, anti-metabolite, and platinum-based drug families...
February 16, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28209170/lincrnafezf1-as1-represses-p21-expression-to-promote-gastric-cancer-proliferation-through-lsd1-mediated-h3k4me2-demethylation
#13
Yan-Wen Liu, Rui Xia, Kai Lu, Min Xie, Fen Yang, Ming Sun, Wei De, Cailian Wang, Guozhong Ji
BACKGROUND: Although the prognosis of gastric cancer patients have a favorable progression, there are some patients with unusual patterns of locoregional and systemic recurrence. Therefore, a better understanding of early molecular events of the disease is needed. Current evidences demonstrate that long noncoding RNAs (lncRNAs) may be an important class of functional regulators involved in human gastric cancers development. Our previous studies suggest that HOTAIR contributes to gastric cancer development, and the overexpression of HOTAIR predicts a poor prognosis...
February 16, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28203520/eukaryotic-translation-initiation-factor-3-subunit-d-overexpression-is-associated-with-the-occurrence-and-development-of-ovarian-cancer
#14
Yaying Lin, Rongrong Zhang, Ping Zhang
Ovarian cancer is the most common cause of gynaecological cancer-associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpressed in ovarian cancer clinical tissues. Furthermore, the expression of EIF3D was correlated with the International Federation of Gynecology and Obstetrics stage and pathological differentiation stage. 3-(4,5-dimethylthylthiazol-2-yl)-2 (MTT) and colony formation assays revealed that the lentivirus-mediated knockdown of EIF3D suppresses cell proliferation in the ovarian tumour cell lines CAOV-3 and SKOV-3...
December 2016: FEBS Open Bio
https://www.readbyqxmd.com/read/28199407/keratin14-mrna-expression-in-human-pneumocytes-during-quiescence-repair-and-disease
#15
Marco Confalonieri, Emanuele Buratti, Gabriele Grassi, Rossana Bussani, Marco Chilosi, Rossella Farra, Michela Abrami, Cristiana Stuani, Francesco Salton, Miriam Ficial, Paola Confalonieri, Lorenzo Zandonà, Maurizio Romano
The lung alveoli slowly self-renew pneumocytes, but their facultative regeneration capacity is rapidly efficient after an injury, so fibrosis infrequently occurs. We recently observed Keratin 14 (KRT14) expression during diffuse alveolar damage (DAD), but not in controls. We wonder if KRT14 may be a marker of pneumocyte transition from quiescence to regeneration. Quantitative PCR and Western blot analyses highlighted the presence of KRT14 (mRNA and protein) only in human lung samples with DAD or interstitial lung disease (ILD)...
2017: PloS One
https://www.readbyqxmd.com/read/28198463/overexpression-of-fam83h-as1-indicates-poor-patient-survival-and-knockdown-impairs-cell-proliferation-and-invasion-via-met-egfr-signaling-in-lung-cancer
#16
Jie Zhang, Shumei Feng, Wenmei Su, Shengbin Bai, Lei Xiao, Lihui Wang, Dafydd G Thomas, Jules Lin, Rishindra M Reddy, Philip W Carrott, William R Lynch, Andrew C Chang, David G Beer, You-Min Guo, Guoan Chen
Whole transcriptome analyses of next generation RNA sequencing (RNA-Seq) data from human cancer samples reveled thousands of uncharacterized non-coding RNAs including long non-coding RNA (lncRNA). Recent studies indicated that lncRNAs are emerging as crucial regulators in cancer processes and potentially useful as biomarkers for cancer diagnosis and prognosis. To delineate dysregulated lncRNAs in lung cancer, we analyzed RNA-Seq data from 461 lung adenocarcinomas (LUAD) and 156 normal lung tissues. FAM83H-AS1, one of the top dysregulated lncRNAs, was found to be overexpressed in tumors relative to normal lung and significantly associated with worse patient survival in LUAD...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197300/long-term-treatment-of-native-ldl-induces-senescence-of-cultured-human-endothelial-cells
#17
Sung-Tack Oh, Hoon Park, Hyun Joong Yoon, Sung Yeul Yang
The study aimed to evaluate whether the treatment of primary cultured human endothelial cells with native low-density lipoprotein (nLDL) could induce their senescence and to uncover some of the putative mechanisms involved. For this purpose, human umbilical vein endothelial cells (HUVECs) were subcultured and/or continuously cultured with nLDL (0, 2, 5, and 10 μg protein/mL), for up to 9 days. The results indicated that nLDL inhibited the proliferation of HUVECs by arresting the cell cycle at G1 phase. The G1-arrested cells showed increase in cytosolic senescence-associated-β-galactosidase (SA-β-Gal) activity, a biomarker of cellular senescence...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28193232/peg10-overexpression-induced-by-e2f-1-promotes-cell-proliferation-migration-and-invasion-in-pancreatic-cancer
#18
Yun-Peng Peng, Yi Zhu, Ling-Di Yin, Jing-Jing Zhang, Ji-Shu Wei, Xian Liu, Xin-Chun Liu, Wen-Tao Gao, Kui-Rong Jiang, Yi Miao
BACKGROUND: Overexpression of paternally expressed gene-10 (PEG10) is known to promote the progression of several carcinomas, however, its role in pancreatic cancer (PC) is unknown. We investigated the expression and function of PEG10 in PC. METHODS: PEG10 expression and correlation with PC progression was assessed in cancerous tissues and paired non-cancerous tissues. Further, the role of PEG10 in PC cell progression and the underlying mechanisms were studied by using small interfering RNA (Si-RNA)...
February 13, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28185406/detecting-senescence-a-new-method-for-an-old-pigment
#19
Hanna Salmonowicz, João F Passos
Cellular senescence is a state of irreversible cell cycle arrest induced by different types of cellular stresses. The field of senescence has made significant advances in the understanding of many of the mechanisms governing this phenomenon; however, a universal biomarker that unambiguously distinguishes senescent from proliferating cells has not been found. In this issue of Aging Cell, Evangelou and colleagues developed a sensitive method for identification of senescent cells in different types of biological material based on the detection of lipofuscin using an analogue of Sudan Black B (SBB) histochemical dye coupled with biotin, which they named GL13...
February 9, 2017: Aging Cell
https://www.readbyqxmd.com/read/28184928/osthole-inhibits-the-tumorigenesis-of-hepatocellular-carcinoma-cells
#20
Zhi-Kun Lin, Jia Liu, Guo-Qiang Jiang, Guang Tan, Peng Gong, Hai-Feng Luo, Hui-Min Li, Jian Du, Zhen Ning, Yi Xin, Zhong-Yu Wang
Hepatocellular carcinoma (HCC) accounts for approximately 90% of all cases of primary liver cancer, and the majority of patients with HCC are deprived of effective curative methods. Osthole is a Chinese herbal medicine which has been reported to possess various pharmacological functions, including hepatocellular protection. In the present study, we investigated the anticancer activity of osthole using HCC cell lines. We found that osthole inhibited HCC cell proliferation, induced cell cycle arrest, triggered DNA damage and suppressed migration in HCC cell lines...
March 2017: Oncology Reports
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