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https://www.readbyqxmd.com/read/28532539/-detection-of-serum-peptides-in-patients-with-lung-squamous-cell-carcinoma-by-maldi-tof-ms-and-analysis-of-their-correlation-with-chemotherapy-efficacy
#1
Guanhua Zhao, Bin Xu, Xiaoyan Li, Chuanhao Tang, Haifeng Qin, Hong Wang, Shaoxing Yang, Weixia Wang, Hongjun Gao, Kun He, Xiaoqing Liu
BACKGROUND: Treatment options for patients with squamous cell carcinoma of the lung (SCC) are limited in chemotherapy. However, not all patients could benefit form standard platinum regimen. Considering the dismal prognosis of patients with advanced SCC, a greater focus on selecting sensitive chemotherapy regimens remains of upmost importance to improve outcomes in this disease. In this study, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to detect pre-chemotherapy serum peptides in advanced lung squamous cell carcinoma patients accepting paclitaxel combined with platinum chemotherapy and to analyze the correlation between serum peptides and chemotherapy efficacy...
May 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28386154/the-exploration-of-peptide-biomarkers-in-malignant-pleural-effusion-of-lung-cancer-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#2
Jing Xu, Bin Xu, Chuanhao Tang, Xiaoyan Li, Haifeng Qin, Weixia Wang, Hong Wang, Zhongyuan Wang, Liangliang Li, Zhihua Li, Hongjun Gao, Kun He, Xiaoqing Liu
Background. Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics. In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE. Material and Methods. The 46 MPE and 32 TPE were randomly assigned to training set and validation set. Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800-10000 Da were analyzed...
2017: Disease Markers
https://www.readbyqxmd.com/read/28360188/rapid-detection-of-cfia-metallo-%C3%AE-lactamase-producing-bacteroides-fragilis-by-the-combination-of-maldi-tof-ms-and-carbanp
#3
Pak-Leung Ho, Chong-Yee Yau, Lok-Yan Ho, Jonathan H K Chen, Eileen L Y Lai, Stephanie W U Lo, Cindy W S Tse, Kin-Hung Chow
AIMS: Carbapenem resistance in Bacteroides fragilis is emerging and is mainly attributed to insertion sequence (IS)-mediated activation of the carbapenemase gene cfiA. We investigated the use of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and the CarbaNP assay for the rapid identification of these strains. METHODS: This study used the Bruker MALDI Biotype system and the mass spectra models generated by 20 B. fragilis reference strains (10 cfiA-positive and 10 cfiA-negative) in the ClinProTools software to identify 404 B...
March 30, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27919649/maldi-tof-ms-identification-of-species-from-the-acinetobacter-baumannii-ab-group-revisited-inclusion-of-the-novel-a-%C3%A2-seifertii-and-a-%C3%A2-dijkshoorniae-species
#4
M Marí-Almirall, C Cosgaya, P G Higgins, A Van Assche, M Telli, G Huys, B Lievens, H Seifert, L Dijkshoorn, I Roca, Jordi Vila
OBJECTIVES: Rapid identification of Acinetobacter species is critical as members of the A. baumannii (Ab) group differ in antibiotic susceptibility and clinical outcomes. A. baumannii, A. pittii, and A. nosocomialis can be identified by MALDI-TOF/MS, while the novel species A. seifertii and A. dijkshoorniae cannot. Low identification rates for A. nosocomialis also have been reported. We evaluated the use of MALDI-TOF/MS to identify isolates of A. seifertii and A. dijkshoorniae and revisited the identification of A...
March 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27888142/profiling-and-identification-of-new-proteins-involved-in-brain-ischemia-using-maldi-imaging-mass-spectrometry
#5
Víctor Llombart, Sebastián Alejandro Trejo, Sílvia Bronsoms, Anna Morancho, Ma Feifei, Júlia Faura, Teresa García-Berrocoso, Alba Simats, Anna Rosell, Francesc Canals, Mar Hernández-Guillamón, Joan Montaner
The identification of proteins involved in brain ischemia might allow the discovery of putative biomarkers or therapeutic targets for ischemic stroke. Our aim is to study the distribution of proteins within mouse brain after an ischemic insult using MALDI imaging-mass-spectrometry and to identify relevant proteins involved in brain damage. We occluded the middle cerebral artery of C57BL/6J mice. Brain slices were analyzed by MALDI-TOF and infarct (IC) and contralateral (CL) regions were compared using ClinProTools...
January 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/27507023/establishment-and-application-of-an-analytical-in-house-database-ihdb-for-rapid-discrimination-of-bacillus-subtilis-group-bsg-using-whole-cell-maldi-tof-ms-technology
#6
Chien-Hsun Huang, Lina Huang, Mu-Tzu Chang, Kuo-Lung Chen
Members of the Bacillus subtilis group (BSG) possess industrial applicability; unfortunately, B. subtilis and its phylogenetically closest species are indistinguishable from one another using 16S rDNA sequencing, physiological and biochemical tests. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a relatively novel technique for the fast and reliable identification of microorganisms. The aim of this study was to construct a unique analytical in-house database (IHDB) for BSG discrimination based on whole-cell protein fingerprinting using MALDI-TOF MS, as well as to discover biomarkers from the MS peaks to generate a classification model for further differentiation using the ClinProTools software...
August 6, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27469535/new-detection-method-in-experimental-mice-for-schistosomiasis-clinprotool-and-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#7
Yuzheng Huang, Wei Li, Kun Liu, Chunrong Xiong, Peng Cao, Jianping Tao
Oncomelania hupensis snails along the Yangtze River and the low positive rate and infectiosity of human and livestock schistosomiasis still pose a threat to public health in China. Adult blood flukes were recognized as Schistosoma japonicum, which are found in the portal system of the sentinel mice bred in the laboratory for 35 days after contact with the water. However, 35 days was too long from the field test to dissection, and the dissection in the laboratory was also time-consuming and labor-intensive...
November 2016: Parasitology Research
https://www.readbyqxmd.com/read/27379069/identification-of-candida-haemulonii-complex-species-use-of-clinprotools-tm-to-overcome-limitations-of-the-bruker-biotyper-tm-vitek-ms-tm-ivd-and-vitek-ms-tm-ruo-databases
#8
Rafaella C Grenfell, Afonso R da Silva Junior, Gilda M B Del Negro, Regina B Munhoz, Viviane M F Gimenes, Diego M Assis, Anna C Rockstroh, Adriana L Motta, Flavia Rossi, Luiz Juliano, Gil Benard, João N de Almeida Júnior
Candida haemulonii is now considered a complex of two species and one variety: C. haemulonii sensu stricto, Candida duobushaemulonii and the variety C. haemulonii var. vulnera. Identification (ID) of these species is relevant for epidemiological purposes and for therapeutic management, but the different phenotypic commercial systems are unable to provide correct species ID for these emergent pathogens. Hence, we evaluated the MALDI-TOF MS performance for the ID of C. haemulonii species, analyzing isolates/strains of C...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27295850/-proteomic-analysis-of-rat-brain-stem-with-dai-by-maldi-tof-ms
#9
Guan-heng Ren, Ning-guo Liu, Yi-jiu Chen, Yan Shi, Dong-hua Zou, Ping Huang, Zheng-dong Li, Ho Yu Sha, Kai-fei Deng
OBJECTIVE: To establish a diagnostic model for diffuse axonal injury (DAI) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). To screen the proteins or peptides associated with DAI for providing the biomarkers with theoretic foundation. METHODS: Fifteen male Sprague-Dawley rats were randomly divided into DAI group (n = 10) and control group (n = 5). The protein or peptide expression profiles of rat brain stem were detected by MALDI-TOF-MS...
February 2016: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/26950986/-maldi-tof-mass-spectrometric-anaiysis-of-leptospira-spp-used-in-serodiagnostics-of-leptospirosis
#10
E V Zyeva, N A Stoyanova, N K Tokarevich, Areg A Totolyan
AIM: Creation of a classification model of Leptospira spp. serovar model using ClinProTools 3.0 software and evaluation of use of MALDI-TOF MS as a method of quality control of reference strains of leptospira. MATERIALS AND METHODS: 10 reference strains of Leptospira spp. were used in the study according to microscopic agglutination reaction from the collection of Pasteur RIEM. All the strains were cultivated for 10 days in Terskikh medium at 28 degrees C. Cell extracts were obtained by ethanol/formic acid method...
November 2015: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii
https://www.readbyqxmd.com/read/26656930/utilization-of-whole-cell-maldi-tof-mass-spectrometry-to-differentiate-burkholderia-pseudomallei-wild-type-and-constructed-mutants
#11
Suthamat Niyompanich, Kitima Srisanga, Janthima Jaresitthikunchai, Sittiruk Roytrakul, Sumalee Tungpradabkul
Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (whole-cell MALDI-TOF MS) has been widely adopted as a useful technology in the identification and typing of microorganisms. This study employed the whole-cell MALDI-TOF MS to identify and differentiate wild-type and mutants containing constructed single gene mutations of Burkholderia pseudomallei, a pathogenic bacterium causing melioidosis disease in both humans and animals. Candidate biomarkers for the B. pseudomallei mutants, including rpoS, ppk, and bpsI isolates, were determined...
2015: PloS One
https://www.readbyqxmd.com/read/26282790/development-and-evaluation-of-maldi-tof-ms-based-serotyping-for-streptococcus-pneumoniae
#12
S Nakano, Y Matsumura, Y Ito, T Fujisawa, B Chang, S Suga, K Kato, T Yunoki, G Hotta, T Noguchi, M Yamamoto, M Nagao, S Takakura, M Ohnishi, T Ihara, S Ichiyama
Surveillance of Streptococcus pneumoniae serotypes is important for the successful implementation of vaccination strategies to prevent the spread of invasive pneumococcal diseases. The standard method of serotyping of pneumococcal isolates is the phenotypic Neufeld test, which is cost- and labor-intensive. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been implemented as a rapid, simple and inexpensive method for identifying species. We evaluated the performance of MALDI-TOF MS for serotyping ten major serotypes of S...
November 2015: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/26253802/analysis-of-methicillin-resistant-staphylococcus-aureus-major-clonal-lineages-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms
#13
Tingting Zhang, Jinya Ding, Xiancai Rao, Jingbo Yu, Meiling Chu, Wei Ren, Lu Wang, Wencheng Xue
Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen associated with nosocomial infections in many countries. Multilocus sequence typing (MLST) is one of the genetic typing methods used to type MRSA with a high discriminatory power, however, it is labor-intensive, timely, and costly. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) coupled with ClinProTools is a potential tool to discover biomarker peaks and to generate a classification model based on highly sophisticated mathematical algorithms to discriminate clonal lineages...
October 2015: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/26164386/the-application-of-fuzzy-statistics-and-linear-discriminant-analysis-as-criteria-for-optimizing-the-preparation-of-plasma-for-matrix-assisted-laser-desorption-ionization-mass-spectrometry-peptide-profiling
#14
Joanna Hajduk, Jan Matysiak, Piotr Kokot, Piotr Nowicki, Paweł Dereziński, Zenon J Kokot
An alternative bioinformatics approach based on fuzzy theory statistics and linear discriminant analysis is proposed for the interpretation of MALDI MS spectra in peptide profiling. When applied, the methodology enables the establishment of a reproducible plasma preparation protocol appropriate for the evaluation of small data sets. The samples were collected from pregnant women affected by gestational diabetes mellitus (GDM), n=18 and control group, n=13. The following pre-treatment sets were tested: pipette tips with C18 stationary phase (ZipTip, Millipore and Omix, Agilent) and magnetic bead-based weak cation exchange chromatography kit (MB WCX, Bruker Daltonics)...
August 25, 2015: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/26156200/effects-of-a-terrified-sound-stress-on-serum-proteomic-profiling-in-mice
#15
Juan Yang, Xin Zhang, Xiaofan Xiong, Qiuhua Wu, Lingyu Zhao, Liying Liu, Yannan Qin, Tusheng Song, Chen Huang
The serum proteomic profiles of mice exposed to terrified-sound-induced stress and after stress release were investigated. Serum samples from 32 mice were divided into four groups (n = 8 each) and analyzed using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry techniques (MALDI-TOF MS) combined with magnetic bead-based weak cation-exchange chromatography. ClinProTools software identified several distinct markers that differed between the stressed and control groups and between the stress released and stressed released controls...
October 2015: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/26047516/classification-of-epidermal-growth-factor-receptor-gene-mutation-status-using-serum-proteomic-profiling-predicts-tumor-response-in-patients-with-stage-iiib-or-iv-non-small-cell-lung-cancer
#16
Lin Yang, Chuanhao Tang, Bin Xu, Weixia Wang, Jianjie Li, Xiaoyan Li, Haifeng Qin, Hongjun Gao, Kun He, Santai Song, Xiaoqing Liu
OBJECTIVES: Epidermal growth factor receptor (EGFR) gene mutations in tumors predict tumor response to EGFR tyrosine kinase inhibitors (EGFR-TKIs) in non-small-cell lung cancer (NSCLC). However, obtaining tumor tissue for mutation analysis is challenging. Here, we aimed to detect serum peptides/proteins associated with EGFR gene mutation status, and test whether a classification algorithm based on serum proteomic profiling could be developed to analyze EGFR gene mutation status to aid therapeutic decision-making...
2015: PloS One
https://www.readbyqxmd.com/read/25972224/use-of-maldi-biotyper-plus-clinprotools-mass-spectra-analysis-for-correct-identification-of-streptococcus-pneumoniae-and-streptococcus-mitis-oralis
#17
Jonathan H K Chen, Kevin K K She, Oi-Ying Wong, Jade L L Teng, Wing-Cheong Yam, Susanna K P Lau, Patrick C Y Woo, Vincent C C Cheng, Kwok-Yung Yuen
BACKGROUND: Differentiation of Streptococcus pneumoniae from other viridans group streptococci is well known to be challenging in clinical laboratories. Matrix assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) had been reported to be a good alternative for Streptococcus species level identification. However, differentiation of S. pneumoniae from other Streptococcus mitis group organisms was found to be problematic using the Bruker MALDI Biotyper system...
August 2015: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/25926500/identification-of-haemophilus-influenzae-type-b-isolates-by-use-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#18
COMPARATIVE STUDY
Viktor Månsson, Fredrik Resman, Markus Kostrzewa, Bo Nilson, Kristian Riesbeck
Haemophilus influenzae type b (Hib) is, in contrast to non-type b H. influenzae, associated with severe invasive disease, such as meningitis and epiglottitis, in small children. To date, accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) provides rapid bacterial diagnostics and is increasingly used in clinical microbiology laboratories. Here, MALDI-TOF MS was evaluated as a novel approach to separate Hib from other H...
July 2015: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/25905870/application-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-in-the-screening-of-vana-positive-enterococcus-faecium
#19
Li-jun Wang, Xin-xin Lu, Wei Wu, Wen-jun Sui, Gui Zhang
In order to evaluate a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MAIDI-TOF MS) assay in screening vancomycin-resistant Enterococcus faecium, a total of 150 E. faecium clinical strains were studied, including 60 vancomycin-resistant E. faecium (VREF) isolates and 90 vancomycin-susceptible (VSEF) strains. Vancomycin resistance genes were detected by sequencing. E. faecium were identified by MALDI-TOF MS. A genetic algorithm model with ClinProTools software was generated using spectra of 30 VREF isolates and 30 VSEF isolates...
2014: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/25673457/identification-of-biomarkers-for-endometriosis-using-clinical-proteomics
#20
Yang Zhao, Ya-Nan Liu, Yi Li, Li Tian, Xue Ye, Heng Cui, Xiao-Hong Chang
BACKGROUND: We investigated possible biomarkers for endometriosis (EM) using the ClinProt technique and proteomics methods. METHODS: We enrolled 50 patients with EM, 34 with benign ovarian neoplasms and 40 healthy volunteers in this study. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) combined with weak cationic exchange (WCX) magnetic beads. Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed, and ClinProtools software, results were refined using online liquid chromatography-tandem MS...
February 20, 2015: Chinese Medical Journal
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