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https://www.readbyqxmd.com/read/29330584/a-dedicated-neurologist-at-the-emergency-department-during-out-of-office-hours-decreases-patients-length-of-stay-and-admission-percentages
#1
M Christien van der Linden, Crispijn L van den Brand, Ido R van den Wijngaard, Roeline A Y de Beaufort, Naomi van der Linden, Korné Jellema
BACKGROUND: Emergency departments (EDs) worldwide face crowding, which hampers patient flow. In this study, the impact of a dedicated neurologist present at the ED on patient flow during out-of-office hours was assessed. METHODS: A cross-sectional, mixed methods study was undertaken at a Dutch ED, including a pre-post analysis of data of patients who had a primary neurological disease (n = 458) and staff surveys (n = 152). Descriptive statistics and content analysis were used for analyses...
January 12, 2018: Journal of Neurology
https://www.readbyqxmd.com/read/29330104/circular-rna-an-emerging-non-coding-rna-as-a-regulator-and-biomarker-in-cancer
#2
Bing Chen, Shenglin Huang
Circular RNA (circRNA) is a type of covalently closed non-coding RNA that may regulate gene expression in eukaryotes. The recent application of high-throughput RNA sequencing and bioinformatics approaches has revealed a large number of circRNAs in human cells. Emerging evidence indicates that many circRNAs have cell-type specific expression and are linked to physiological development and various diseases. Specially, circRNAs can either serve as oncogenic stimuli or tumor suppressors in cancer. circRNAs have also been shown to be enriched and stable in extracellular fluid, indicating the potential of circRNAs as cancer biomarkers...
January 9, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29329935/tick-borne-pathogen-detection-what-s-new
#3
Alejandro Cabezas-Cruz, Muriel Vayssier-Taussat, Gilbert Greub
Ticks and the pathogens they transmit constitute a growing burden for human and animal health worldwide. Traditionally, tick-borne pathogen detection has been carried out using PCR-based methods that rely in known sequences for specific primers design. This approach matches with the view of a 'single-pathogen' epidemiology. Recent results, however, have stressed the importance of coinfections in pathogen ecology and evolution with impact in pathogen transmission and disease severity. New approaches, including high-throughput technologies, were then used to detect multiple pathogens, but they all need a priori information on the pathogens to search...
January 9, 2018: Microbes and Infection
https://www.readbyqxmd.com/read/29328319/dynamic-spatial-filtering-using-a-digital-micromirror-device-for-high-speed-optical-diffraction-tomography
#4
Di Jin, Renjie Zhou, Zahid Yaqoob, Peter T C So
Optical diffraction tomography (ODT) is an emerging microscopy technique for three-dimensional (3D) refractive index (RI) mapping of transparent specimens. Recently, the digital micromirror device (DMD) based scheme for angle-controlled plane wave illumination has been proposed to improve the imaging speed and stability of ODT. However, undesired diffraction noise always exists in the reported DMD-based illumination scheme, which leads to a limited contrast ratio of the measurement fringe and hence inaccurate RI mapping...
January 8, 2018: Optics Express
https://www.readbyqxmd.com/read/29325623/tourette-disorder-and-other-tic-disorders
#5
Thomas V Fernandez, Matthew W State, Christopher Pittenger
Tourette disorder is a developmental neuropsychiatric condition characterized by vocal and motor tics that can range in severity from mild to disabling. It represents one end of a spectrum of tic disorders and is estimated to affect 0.5-0.7% of the population. Accumulated evidence supports a substantial genetic contribution to disease risk, but the identification of genetic variants that confer risk has been challenging. Positive findings in candidate gene association studies have not replicated, and genomewide association studies have not generated signals of genomewide significance, in large part because of inadequate sample sizes...
2018: Handbook of Clinical Neurology
https://www.readbyqxmd.com/read/29321307/kaposi-s-sarcoma-associated-herpesvirus-k8-is-an-rna-binding-protein-that-regulates-viral-dna-replication-in-coordination-with-a-noncoding-rna
#6
Dongcheng Liu, Yan Wang, Yan Yuan
KSHV lytic replication and constant primary infection of fresh cells are crucial for viral tumorigenicity. Virus-encoded b-Zip family protein K8 plays an important role in viral DNA replication in both viral reactivation and de novo infection. The mechanism underlying the functional role of K8 in the viral life cycle is elusive. Here we report that K8 is a RNA binding protein, which also associates with many proteins including other RNA binding proteins. Many K8-involved protein-protein interactions are mediated by RNA...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29320478/an-extracellular-network-of-arabidopsis-leucine-rich-repeat-receptor-kinases
#7
Elwira Smakowska-Luzan, G Adam Mott, Katarzyna Parys, Martin Stegmann, Timothy C Howton, Mehdi Layeghifard, Jana Neuhold, Anita Lehner, Jixiang Kong, Karin Grünwald, Natascha Weinberger, Santosh B Satbhai, Dominik Mayer, Wolfgang Busch, Mathias Madalinski, Peggy Stolt-Bergner, Nicholas J Provart, M Shahid Mukhtar, Cyril Zipfel, Darrell Desveaux, David S Guttman, Youssef Belkhadir
The cells of multicellular organisms receive extracellular signals using surface receptors. The extracellular domains (ECDs) of cell surface receptors function as interaction platforms, and as regulatory modules of receptor activation. Understanding how interactions between ECDs produce signal-competent receptor complexes is challenging because of their low biochemical tractability. In plants, the discovery of ECD interactions is complicated by the massive expansion of receptor families, which creates tremendous potential for changeover in receptor interactions...
January 10, 2018: Nature
https://www.readbyqxmd.com/read/29316089/an-oxygen-tolerant-pet-raft-polymerization-for-screening-structure-activity-relationships
#8
Adam J Gormley, Jonathan Yeow, Gervase Ng, Órla Conway, Cyrille Boyer, Robert Chapman
The complexity of polymer-protein interactions makes rational design of the best polymer architecture for any given biointerface extremely challenging, and the high throughput synthesis and screening of polymers has emerged as an attractive alternative. A porphyrin-catalysed photoinduced electron/energy transfer-reversible addition-fragmentation chain-transfer (PET-RAFT) polymerisation was adapted to enable high throughput synthesis of complex polymer architectures in dimethyl sulfoxide (DMSO) on low-volume well plates in the presence of air...
January 9, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29315082/chemically-linked-metal-matrix-nanocomposites-of-boron-nitride-nanosheets-and-silver-as-thermal-interface-materials
#9
Nirup Nagabandi, Cengiz Yegin, Xuhui Feng, Charles King, Jun Kyun Oh, Ethan Adam Scholar, Sreekant Narumanchi, Mustafa Akbulut
Herein, novel hybrid nanocomposite thermal interface materials (TIMs) relying on the chemical linkage of silver, boron nitride nanosheets (BNNSs), and organic ligands are reported. These TIMs were prepared using a co-electrodeposition/chemisorption approach where the electrolytic reduction of silver ions into silver nano-/micro-crystals was coupled with the conjugation of ligand-coated nanosheets onto silver crystals. Furthermore, the influence of bond strength of silver/nanosheet links on the thermal, mechanical, and structural properties is investigated using a combination of techniques; including laser flash analysis, phase-sensitive transient thermoreflectance, nanoindentation, and electron microscopy...
January 9, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29305293/beyond-screening-for-chromosomal-abnormalities-advances-in-non-invasive-diagnosis-of-single-gene-disorders-and-fetal-exome-sequencing
#10
REVIEW
Jane Hayward, Lyn S Chitty
Emerging genomic technologies, largely based around next generation sequencing (NGS), are offering new promise for safer prenatal genetic diagnosis. These innovative approaches will improve screening for fetal aneuploidy, allow definitive non-invasive prenatal diagnosis (NIPD) of single gene disorders at an early gestational stage without the need for invasive testing, and improve our ability to detect monogenic disorders as the aetiology of fetal abnormalities. This presents clinicians and scientists with novel challenges as well as opportunities...
January 2, 2018: Seminars in Fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29304755/computational-approach-to-discriminate-human-and-mouse-sequences-in-patient-derived-tumour-xenografts
#11
Maurizio Callari, Ankita Sati Batra, Rajbir Nath Batra, Stephen-John Sammut, Wendy Greenwood, Harry Clifford, Colin Hercus, Suet-Feung Chin, Alejandra Bruna, Oscar M Rueda, Carlos Caldas
BACKGROUND: Patient-Derived Tumour Xenografts (PDTXs) have emerged as the pre-clinical models that best represent clinical tumour diversity and intra-tumour heterogeneity. The molecular characterization of PDTXs using High-Throughput Sequencing (HTS) is essential; however, the presence of mouse stroma is challenging for HTS data analysis. Indeed, the high homology between the two genomes results in a proportion of mouse reads being mapped as human. RESULTS: In this study we generated Whole Exome Sequencing (WES), Reduced Representation Bisulfite Sequencing (RRBS) and RNA sequencing (RNA-seq) data from samples with known mixtures of mouse and human DNA or RNA and from a cohort of human breast cancers and their derived PDTXs...
January 5, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29304003/a-high-resolution-proteomic-landscaping-of-primary-human-dental-stem-cells-identification-of-shed-and-pdlsc-specific-biomarkers
#12
Vasiliki Taraslia, Stefania Lymperi, Vasiliki Pantazopoulou, Athanasios K Anagnostopoulos, Issidora S Papassideri, Efthimia K Basdra, Marianna Bei, Evangelos G Kontakiotis, George Th Tsangaris, Dimitrios J Stravopodis, Ema Anastasiadou
Dental stem cells (DSCs) have emerged as a promising tool for basic research and clinical practice. A variety of adult stem cell (ASC) populations can be isolated from different areas within the dental tissue, which, due to their cellular and molecular characteristics, could give rise to different outcomes when used in potential applications. In this study, we performed a high-throughput molecular comparison of two primary human adult dental stem cell (hADSC) sub-populations: Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Periodontal Ligament Stem Cells (PDLSCs)...
January 5, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29302577/rna-binding-protein-as-an-emerging-therapeutic-target-for-cancer-prevention-and-treatment
#13
REVIEW
Suntaek Hong
After transcription, RNAs are always associated with RNA binding proteins (RBPs) to perform biological activities. RBPs can interact with target RNAs in sequence- and structure-dependent manner through their unique RNA binding domains. In development and progression of carcinogenesis, RBPs are aberrantly dysregulated in many human cancers with various mechanisms, such as genetic alteration, epigenetic change, noncoding RNA-mediated regulation, and post-translational modifications. Upon deregulation in cancers, RBPs influence every step in the development and progression of cancer, including sustained cell proliferation, evasion of apoptosis, avoiding immune surveillance, inducing angiogenesis, and activating metastasis...
December 2017: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/29298247/the-effect-of-early-knowledge-of-respiratory-syncytial-virus-positivity-on-medical-decision-making-and-throughput-time-within-the-pediatric-emergency-department
#14
Jessica Schnell, Lisa Schroeder, Kelly Sinclair, Lina Patel, Denise Dowd
OBJECTIVE: The aim of this study was to evaluate whether early knowledge of respiratory syncytial virus (RSV) positivity by rapid triage-based testing protocol improved emergency department (ED) throughput time and decreased ED ancillary testing and antibiotic administration. METHODS: A retrospective cohort study was performed of ED patients aged 2 to 12 months, presenting November 1 to March 31 for the years 2008-2013. Included patients presented with fever and upper respiratory tract symptoms...
January 2, 2018: Pediatric Emergency Care
https://www.readbyqxmd.com/read/29290623/identification-of-new-activators-of-mitochondrial-fusion-reveals-a-link-between-mitochondrial-morphology-and-pyrimidine-metabolism
#15
Laia Miret-Casals, David Sebastián, José Brea, Eva M Rico-Leo, Manuel Palacín, Pedro M Fernández-Salguero, M Isabel Loza, Fernando Albericio, Antonio Zorzano
Mitochondria are dynamic organelles that produce most of the cellular ATP, and are involved in many other cellular functions such as Ca2+ signaling, differentiation, apoptosis, cell cycle, and cell growth. One key process of mitochondrial dynamics is mitochondrial fusion, which is catalyzed by mitofusins (MFN1 and MFN2) and OPA1. The outer mitochondrial membrane protein MFN2 plays a relevant role in the maintenance of mitochondrial metabolism, insulin signaling, and mutations that cause neurodegenerative disorders...
December 23, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/29289327/rapid-determination-of-ultra-trace-plutonium-isotopes-239pu-240pu-and-241pu-in-small-volume-human-urine-bioassay-using-sector-field-inductively-coupled-plasma-mass-spectrometry
#16
Youyi Ni, Jian Zheng, Qiuju Guo, Wu Men, Keiko Tagami, Shigeo Uchida
A rapid method with enhanced 238U decontamination was developed for ultra-trace Pu analysis in small-volume urine bioassays. This method consists of acid digestion, co-precipitation, extraction chromatography and sector-field inductively coupled plasma mass spectrometry (SF-ICP-MS) measurement. Parameters that may influence the analytical performance were studied systematically. This method achieved a high 238U decontamination factor (3.8 × 106) and the 242Pu recovery was stable for 20 mL and 100 mL urine bioassays with an average value of 72...
February 13, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29288699/development-of-a-replicon-cell-line-based-high-throughput-antiviral-assay-for-screening-inhibitors-of-zika-virus
#17
Jia-Qi Li, Cheng-Lin Deng, Dayong Gu, Xiao Li, Lei Shi, Jian'an He, Qiu-Yan Zhang, Bo Zhang, Han-Qing Ye
Zika virus (ZIKV) is an important emerging human pathogen associated with microcephaly, Guillain-Barré syndrome and meningoencephalitis. Developing rapid and reliable HTS assay is important for ZIKV drug discovery. Here, we constructed a dicistronic ZIKV replicon (ZIKV-Pac-Rluc-Rep) that contained the Renilla luciferase (Rluc) reporter gene separated from the puromycin N-acetyl-transferase (Pac) selectable marker by a short peptide cleavage site. A clonal replicon cell line stably expressing high level of ZIKV replicon was established by selection with puromycin...
December 27, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29288089/exploring-the-molecular-complexity-of-triatoma-dimidiata-sialome
#18
Paula Beatriz Santiago, Carla Nunes de Araújo, Sébastien Charneau, Izabela Marques Dourado Bastos, Teresa Cristina F Assumpção, Rayner Myr Lauterjung Queiroz, Yanna Reis Praça, Thuany de Moura Cordeiro, Carlos Henrique Saraiva Garcia, Ionizete Garcia da Silva, Tainá Raiol, Flávia Nader Motta, João Victor de Araújo Oliveira, Marcelo Valle de Sousa, José Marcos C Ribeiro, Jaime Martins de Santana
Triatoma dimidiata, a Chagas disease vector widely distributed along Central America, has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in peridomestic areas fed human blood. Exploring the salivary compounds that overcome host haemostatic and immune responses is of great scientific interest. Here, we provide a deeper insight into its salivary gland molecules. We used high-throughput RNA sequencing to examine in depth the T. dimidiata salivary gland transcriptome...
December 26, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29287474/practical-considerations-challenges-and-limitations-of-bioconjugation-via-azide-alkyne-cycloaddition
#19
Chad J Pickens, Stephanie N Johnson, Melissa M Pressnall, Martin Leon, Cory Berkland
Interrogating biological systems is often limited by access to biological probes. The emergence of "click chemistry" has revolutionized bioconjugate chemistry by providing facile reaction conditions amenable to both biologic molecules and small molecule probes such as fluorophores, toxins, or therapeutics. One particularly popular version is the copper-catalyzed azide-alkyne cycloaddition (AAC) reaction, which has spawned new alternatives such as the strain-promoted azide-alkyne cycloaddition reaction, among others...
December 29, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29287234/improving-the-discovery-of-secondary-metabolite-natural-products-using-ion-mobility-mass-spectrometry
#20
REVIEW
Alexandra C Schrimpe-Rutledge, Stacy D Sherrod, John A McLean
Secondary metabolite discovery requires an unbiased, comprehensive workflow to detect unknown unknowns for which little to no molecular knowledge exists. Untargeted mass spectrometry-based metabolomics is a powerful platform, particularly when coupled with ion mobility for high-throughput gas-phase separations to increase peak capacity and obtain gas-phase structural information. Ion mobility data are described by the amount of time an ion spends in the drift cell, which is directly related to an ion's collision cross section (CCS)...
December 26, 2017: Current Opinion in Chemical Biology
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