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https://www.readbyqxmd.com/read/28738622/a-flow-based-procedure-exploiting-the-lab-in-syringe-approach-for-the-determination-of-ester-content-in-biodiesel-and-diesel-biodiesel-blends
#1
Samara Soares, Wanessa R Melchert, Fábio R P Rocha
The ester content is an important parameter to be monitored in biodiesel for evaluation of the transesterification reaction yield and for assessing the purity of the final product. This is also a relevant quality parameter in diesel/biodiesel blends to avoid frauds, because legislation establishes a minimum amount of biodiesel to be added to diesel. The official method EN14103 requires the addition of an alternative internal standard (methyl nonadecanoate) for analysis of biodiesel from bovine tallow because the methyl heptadecanoate is found in high amounts in this product...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28731431/association-between-cholesterol-exposure-and-neuropathological-findings-the-act-study
#2
Brianne M Bettcher, M Colin Ard, Bruce R Reed, Andreana Benitez, Amanda Simmons, Eric B Larson, Josh A Sonnen, Thomas J Montine, Ge Li, C Dirk Keene, Paul K Crane, Dan Mungas
We characterized the relationship between late life cholesterol exposure and neuropathological outcomes in a community-based, older adult cohort. Adult Changes in Thought (ACT) is a cohort study that enrolls consenting, randomly selected, non-demented people aged ≥65 from a healthcare delivery system. We used late life HDL and total cholesterol lab values from Group Health computerized records, and calculated HDL and non-HDL levels. We evaluated neuropathological outcomes of Alzheimer's disease, cerebral amyloid angiopathy, vascular brain injury, and Lewy body disease...
July 19, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28730699/elevated-cardiac-troponin-t-in-cirrhotic-patients-with-emergency-care-admissions-is-associated-with-mortality
#3
Mayada Elnegouly, Katrin Umgelter, Wajima Safi, Alexander Hapfelmeier, Roland M Schmid, Andreas Umgelter
BACKGROUND & AIMS: Mortality of cirrhotic patients after emergency care admission is high and prognostic factors can help in prioritizing patients. The aim of our study was to assess the association between levels of cardiac Troponin T (cTnT) and one-year mortality in patients with liver cirrhosis without known cardiac disease, who were admitted to the emergency department (ED). METHODS: All patients with cirrhosis presented to the ED from October 2009 until August 2015 who had an initial cTnT value measured with the first lab-panel were retrospectively analyzed with a follow-up of 365 days...
July 20, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28728399/fermentation-quality-and-in-vitro-methane-production-of-sorghum-silage-prepared-with-cellulase-and-lactic-acid-bacteria
#4
Waroon Khota, Suradej Pholsen, David Higgs, Cai Yimin
Objective: The effects of lactic acid bacteria (LAB) and cellulase enzyme on fermentation quality, microorganism population, chemical composition and in vitro gas production of sorghum silages were studied. Methods: Commercial inoculant Lactobacillus plantarum Chikuso 1 (CH), local selected strain L. casei TH 14 and Acremonium cellulase (AC) were used as additives in sorghum silage preparation. Results: Prior to ensiling Sorghum contained 104 LAB and 106 cfu/g fresh matter coliform bacteria...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28727608/evaluation-of-non-invasive-hemoglobin-monitoring-in-trauma-patients-with-low-hemoglobin-levels
#5
Medhat Gamal, Bassant Abdelhamid, Dina Zakaria, Omnia Abd El Dayem, Ashraf Rady, Maher Fawzy, Ahmed Hasanin
OBJECTIVE: Bleeding is a leading cause of death among trauma patients. Delayed assessment of blood hemoglobin level might result in either unnecessary blood transfusion in non-indicated patients or delayed blood transfusion in critically bleeding patients. In this study, we evaluate the precision of non-invasive hemoglobin monitoring in trauma patients with low hemoglobin levels. METHODS: We included trauma patients with low hemoglobin levels (less than 8 g/dL) scheduled for surgical intervention...
July 19, 2017: Shock
https://www.readbyqxmd.com/read/28725789/improving-american-healthcare-through-clinical-lab-2-0-a-project-santa-fe-report
#6
James M Crawford, Khosrow Shotorbani, Gaurav Sharma, Michael Crossey, Tarush Kothari, Thomas S Lorey, Jeffrey W Prichard, Myra Wilkerson, Nancy Fisher
Project Santa Fe was established both to provide thought leadership and to help develop the evidence base for the valuation of clinical laboratory services in the next era of American healthcare. The participants in Project Santa Fe represent major regional health systems that can operationalize laboratory-driven innovations and test their valuation in diverse regional marketplaces in the United States. We provide recommendations from the inaugural March 2016 meeting of Project Santa Fe. Specifically, in the transition from volume-based to value-based health care, clinical laboratories are called upon to provide programmatic leadership in reducing total cost of care through optimization of time-to-diagnosis and time-to-effective therapeutics, optimization of care coordination, and programmatic support of wellness care, screening, and monitoring...
January 2017: Acad Pathol
https://www.readbyqxmd.com/read/28725175/how-different-eeg-references-influence-sensor-level-functional-connectivity-graphs
#7
Yunzhi Huang, Junpeng Zhang, Yuan Cui, Gang Yang, Ling He, Qi Liu, Guangfu Yin
Highlights: Hamming Distance is applied to distinguish the difference of functional connectivity networkThe orientations of sources are testified to influence the scalp Functional Connectivity Graph (FCG) from different references significantlyREST, the reference electrode standardization technique, is proved to have an overall stable and excellent performance in variable situations. The choice of an electroencephalograph (EEG) reference is a practical issue for the study of brain functional connectivity. To study how EEG reference influence functional connectivity estimation (FCE), this study compares the differences of FCE resulting from the different references such as REST (the reference electrode standardization technique), average reference (AR), linked mastoids (LM), and left mastoid references (LR)...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28712550/towards-an-automatic-lab-on-valve-ion-mobility-spectrometric-system-for-detection-of-cocaine-abuse
#8
David J Cocovi-Solberg, Francesc A Esteve-Turrillas, Sergio Armenta, Miguel de la Guardia, Manuel Miró
A lab-on-valve miniaturized system integrating on-line disposable micro-solid phase extraction has been interfaced with ion mobility spectrometry for the accurate and sensitive determination of cocaine and ecgonine methyl ester in oral fluids. The method is based on the automatic loading of 500μL of oral fluid along with the retention of target analytes and matrix clean-up by mixed-mode cationic/reversed-phase solid phase beads, followed by elution with 100μL of 2-propanol containing (3% v/v) ammonia, which are online injected into the IMS...
June 30, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28710950/citric-acid-assisted-phytoextraction-of-chromium-by-sunflower-morpho-physiological-and-biochemical-alterations-in-plants
#9
Mujahid Farid, Shafaqat Ali, Muhammad Rizwan, Qasim Ali, Farhat Abbas, Syed Asad Hussain Bukhari, Rashid Saeed, Longhua Wu
Soil and water contamination from heavy metals and metalloids is one of the most discussed and burning global issues due to its potential to cause the scarcity of healthy food and safe water. The scientific community is proposing a range of lab and field based physical, chemical and biological solutions to remedy metals and metalloids contaminated soils and water. The present study finds out a possibility of Chromium (Cr) extraction by sunflower from spiked soil under chelating role of citric acid (CA). The sunflower plants were grown under different concentrations of Cr (0, 5, 10 & 20mgkg(-1)) and CA (0, 2...
July 12, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28710631/bioconversion-of-phytosterols-into-androstadienedione-by-mycobacterium-smegmatis-cect-8331
#10
Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, Beatriz Galán
The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc(2)155 using phytosterols as raw material in a lab scale...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28707084/a-novel-plant-dna-extraction-method-using-filter-paper-based-96-well-spin-plate
#11
Rui Shi, Dilip R Panthee
A simple, low cost and safe method using homemade filter paper-based 96-well spin plates and homemade solutions was developed for high throughput plant DNA extraction to use in molecular marker analysis. A low cost and safe method was developed for high throughput extraction of plant DNA for molecular marker analysis. In this method, we describe a simple way to prepare 96-well spin plate using filter paper, a plant material product for DNA binding. Such filter paper-based spin plate can combine with homemade non-toxic buffers for high throughput extraction of plant DNA...
July 13, 2017: Planta
https://www.readbyqxmd.com/read/28702080/investigation-of-poly-%C3%AE-glutamic-acid-production-via-online-determination-of-viscosity-and-oxygen-transfer-rate-in-shake-flasks
#12
Lena Regestein Née Meissner, Julia Arndt, Thomas G Palmen, Tim Jestel, Hitoshi Mitsunaga, Eiichiro Fukusaki, Jochen Büchs
BACKGROUND: Poly(γ-glutamic acid) (γ-PGA) is a biopolymer with many useful properties making it applicable for instance in food and skin care industries, in wastewater treatment, in biodegradable plastics or in the pharmaceutical industry. γ-PGA is usually produced microbially by different Bacillus spp. The produced γ-PGA increases the viscosity of the fermentation broth. In case of shake flask fermentations, this results in an increase of the volumetric power input. The power input in shake flasks can be determined by measuring the torque of an orbitally rotating lab shaker...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28701082/bovine-mastitis-prevention-humoral-and-cellular-response-of-dairy-cows-inoculated-with-lactic-acid-bacteria-at-the-dry-off-period
#13
M Pellegrino, N Berardo, J Giraudo, M E F Nader-Macías, C Bogni
The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied...
July 13, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28692541/the-value-of-point-of-care-cd4-and-laboratory-viral-load-in-tailoring-art-monitoring-strategies-to-resource-limitations
#14
Emily P Hyle, Ilesh V Jani, Katherine L Rosettie, Robin Wood, Benjamin Osher, Stephen Resch, Pamela P Pei, Paolo Maggiore, Kenneth A Freedberg, Trevor Peter, Robert A Parker, Rochelle P Walensky
OBJECTIVE: To examine the clinical and economic value of point-of-care CD4 (POC-CD4) or viral load (VL) monitoring compared to current practices in Mozambique, a country representative of the diverse resource limitations encountered by HIV treatment programs in sub-Saharan Africa. DESIGN/METHODS: We use the CEPAC-I model to examine the clinical impact, cost (2014 US$), and incremental cost-effectiveness ratio (ICER, $/year of life saved [YLS]) of ART monitoring strategies in Mozambique...
July 7, 2017: AIDS
https://www.readbyqxmd.com/read/28689457/survey-on-acrylamide-in-roasted-coffee-and-barley-and-in-potato-crisps-sold-in-italy-by-a-lc-ms-ms-method
#15
Terenzio Bertuzzi, Silvia Rastelli, Annalisa Mulazzi, Amedeo Pietri
A survey on the occurrence of acrylamide (AA) in roasted coffee, barley and potato crisps was carried out using an intra-lab validated LC-MS/MS method. Over the years 2015-2016, 66 samples of coffee, 22 of roasted barley and 22 of potato crisps were collected from retail outlets in Italy. AA was detected in almost all samples. In roasted coffee, the level exceeded 450 µg kg(-1), the limit recommended by the European Commission (EC), in 36.4% of the samples. In roasted barley, mean contamination was slightly lower than in coffee and no sample exceeded the EC limit of 2000 µg kg(-1)...
July 10, 2017: Food Additives & Contaminants. Part B, Surveillance
https://www.readbyqxmd.com/read/28687946/genome-based-reclassification-of-fusobacterium-nucleatum-subspecies-at-the-species-level
#16
Joong-Ki Kook, Soon-Nang Park, Yun Kyong Lim, Eugene Cho, Eojin Jo, Hansung Roh, Yeseul Shin, Jayoung Paek, Hwa-Sook Kim, Hongik Kim, Jeong Hwan Shin, Young-Hyo Chang
Fusobacterium nucleatum is classified as four subspecies, subsp. nucleatum, polymorphum, vincentii, and animalis, based on DNA-DNA hybridization (DDH) patterns, phenotypic characteristics, and/or multilocus sequence analysis (MLSA). The gold standards for classification of bacterial species are DDH and 16S ribosomal RNA gene (16S rDNA) sequence homology. The thresholds of DDH and 16S rDNA similarity for delineation of bacterial species have been suggested to be >70 and 98.65%, respectively. Average nucleotide identity (ANI) and genome-to-genome distance (GGD) analysis based on genome sequences were recently introduced as a replacement for DDH to delineate bacterial species with ANI (95-96%) and GGD (70%) threshold values...
July 7, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28682097/saccadic-reaction-times-in-infants-and-adults-spatiotemporal-factors-gender-and-interlaboratory-variation
#17
Ben Kenward, Felix-Sebastian Koch, Linda Forssman, Julia Brehm, Ida Tidemann, Annette Sundqvist, Carin Marciszko, Tone Kristine Hermansen, Mikael Heimann, Gustaf Gredebäck
Saccade latency is widely used across infant psychology to investigate infants' understanding of events. Interpreting particular latency values requires knowledge of standard saccadic RTs, but there is no consensus as to typical values. This study provides standard estimates of infants' (n = 194, ages 9 to 15 months) saccadic RTs under a range of different spatiotemporal conditions. To investigate the reliability of such standard estimates, data is collected at 4 laboratories in 3 countries. Results indicate that reactions to the appearance of a new object are much faster than reactions to the deflection of a currently fixated moving object; upward saccades are slower than downward or horizontal saccades; reactions to more peripheral stimuli are much slower; and this slowdown is greater for boys than girls...
July 6, 2017: Developmental Psychology
https://www.readbyqxmd.com/read/28681129/simultaneous-production-of-intracellular-triacylglycerols-and-extracellular-polyol-esters-of-fatty-acids-by-rhodotorula-babjevae-and-rhodotorula-aff-paludigena
#18
Luis A Garay, Irnayuli R Sitepu, Tomas Cajka, Erin Cathcart, Oliver Fiehn, J Bruce German, David E Block, Kyria L Boundy-Mills
Microbial oils have been analyzed as alternatives to petroleum. However, just a handful of microbes have been successfully adapted to produce chemicals that can compete with their petroleum counterparts. One of the reasons behind the low success rate is the overall economic inefficiency of valorizing a single product. This study presents a lab-scale analysis of two yeast species that simultaneously produce multiple high-value bioproducts: intracellular triacylglycerols (TG) and extracellular polyol esters of fatty acids (PEFA), two lipid classes with immediate applications in the biofuels and surfactant industries...
July 5, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28679447/in-vitro-inhibition-and-reversal-of-plasmodium-falciparum-cytoadherence-to-endothelium-by-monoclonal-antibodies-to-icam-1-and-cd36
#19
Khairul M F Mustaffa, Janet Storm, Megan Whittaker, Tadge Szestak, Alister G Craig
BACKGROUND: Sequestration of parasitized red blood cells from the peripheral circulation during an infection with Plasmodium falciparum is caused by an interaction between the parasite protein PfEMP1 and receptors on the surface of host endothelial cells, known as cytoadherence. Several lines of evidence point to a link between the pathology of severe malaria and cytoadherence, therefore blocking adhesion receptors involved in this process could be a good target to inhibit pRBC sequestration and prevent disease...
July 5, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28679374/development-of-a-counterselectable-seamless-mutagenesis-system-in-lactic-acid-bacteria
#20
Yongping Xin, Tingting Guo, Yingli Mu, Jian Kong
BACKGROUND: Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient. To address this problem, several counterselectable markers have been developed, while few of them could be used in the wild-type host cells without pretreatment. RESULTS: The pheS gene encoding phenylalanyl-tRNA synthetase alpha subunit was identified in Lactococcus lactis NZ9000 genome...
July 5, 2017: Microbial Cell Factories
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