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https://www.readbyqxmd.com/read/28447513/clinical-utility-of-different-anti-m%C3%A3-llerian-hormone-amh-assays-for-the-purpose-of-pregnancy-prediction
#1
Joanna Liss, Michal Kunicki, Adam Czyzyk, Ewa Pastuszek, Judyta Zabielska, Blazej Meczekalski, Krzysztof Lukaszuk
PURPOSE: Comparison of outcomes of IVF cycles where the AMH levels was measured with five different AMH kits: Immunotech (IOT), Beckman Coulter II Gen. RUO, Beckman Coulter II Gen. IVD (BC II IVD), Ansh Labs ultrasensitive (Ansh) and the automated Elecsys Roche assay. METHODS: Retrospective analysis of clinical data for 3693 cycles. RESULTS: In women < 35 years with low (<0.6 ng/ml) and high (>1.4 ng/ml) AMH concentrations, and in those > 39 years with medium (≥0...
April 27, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28439679/screening-lactic-acid-bacteria-strains-with-ability-to-bind-di-n-butyl-phthalate-via-turbiscan-technique
#2
Zhao Lili, Zhao Hongfei, Sana Shoukat, Zhang Xiaochen, Zhang Bolin
Di-n-butyl phthalate (DBP) is a ubiquitous environmental contaminant that poses a risk to humans. Previous work indicates that the ability of lactic acid bacteria (LAB) to bind phthalic acid esters is strain-specific. As cell suspensions of LAB strains in aqueous solution are likely to be colloidal dispersions, this study provided a technique to efficiently screen LAB strains that bind DBP via Turbiscan, which has been widely used to measure the stability of emulsions or colloidal dispersions. Eleven LAB strains belonging to Lactobacillus plantarum, Lb...
April 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28439451/automated-identification-of-insect-vectors-of-chagas-disease-in-brazil-and-mexico-the-virtual-vector-lab
#3
Rodrigo Gurgel-Gonçalves, Ed Komp, Lindsay P Campbell, Ali Khalighifar, Jarrett Mellenbruch, Vagner José Mendonça, Hannah L Owens, Keynes de la Cruz Felix, A Townsend Peterson, Janine M Ramsey
Identification of arthropods important in disease transmission is a crucial, yet difficult, task that can demand considerable training and experience. An important case in point is that of the 150+ species of Triatominae, vectors of Trypanosoma cruzi, causative agent of Chagas disease across the Americas. We present a fully automated system that is able to identify triatomine bugs from Mexico and Brazil with an accuracy consistently above 80%, and with considerable potential for further improvement. The system processes digital photographs from a photo apparatus into landmarks, and uses ratios of measurements among those landmarks, as well as (in a preliminary exploration) two measurements that approximate aspects of coloration, as the basis for classification...
2017: PeerJ
https://www.readbyqxmd.com/read/28435491/agreement-between-serum-assays-performed-in-ed-point-of-care-and-hospital-central-laboratories
#4
Meir Dashevsky, Steven L Bernstein, Carol L Barsky, Richard A Taylor
INTRODUCTION: Point-of-care (POC) testing allows for more time-sensitive diagnosis and treatment in the emergency department (ED) than sending blood samples to the hospital central laboratory (CL). However, many ED patients have blood sent to both, either out of clinical custom, or because clinicians do not trust the POC values. The objective of this study was to examine the level of agreement between POC and CL values in a large cohort of ED patients. METHODS: In an urban, Level I ED that sees approximately 120,000 patients/year, all patients seen between March 1, 2013, and October 1, 2014, who had blood sent to POC and CL labs had levels of agreement measured between serum sodium, potassium, blood urea nitrogen (BUN), creatinine, and hematocrit...
April 2017: Western Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28431357/soil-organic-carbon-and-nitrogen-pools-drive-soil-c-co2-emissions-from-selected-soils-in-maritime-antarctica
#5
C V Pires, C E R G Schaefer, A K Hashigushi, A Thomazini, E I F Filho, E S Mendonça
The ongoing trend of increasing air temperatures will potentially affect soil organic matter (SOM) turnover and soil C-CO2 emissions in terrestrial ecosystems of Maritime Antarctica. The effects of SOM quality on this process remain little explored. We evaluated (i) the quantity and quality of soil organic matter and (ii) the potential of C release through CO2 emissions in lab conditions in different soil types from Maritime Antarctica. Soil samples (0-10 and 10-20cm) were collected in Keller Peninsula and the vicinity of Arctowski station, to determine the quantity and quality of organic matter and the potential to emit CO2 under different temperature scenarios (2, 5, 8 and 11°C) in lab...
April 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28431310/characterization-of-bacterial-communities-of-donkey-milk-by-high-throughput-sequencing
#6
Maria de Los Dolores Soto Del Rio, Alessandra Dalmasso, Tiziana Civera, Maria Teresa Bottero
The interest in donkey milk (DM) is growing because of its functional properties and nutritional value, especially for children with allergies and food intolerances. However, most of the available reports of DM microbiota are based on culture-dependent methods to investigate food safety issues and the presence of lactic acid bacteria (LAB). The aim of this study was to determine the composition of DM bacterial communities using a high-throughput sequencing (HTS) approach. Bulk milk samples from Italian donkey dairy farms from two consecutive years were analysed using the MiSeq Illumina platform...
March 30, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28426942/polychlorinated-biphenyl-and-polybrominated-diphenyl-ether-profiles-in-serum-from-cattle-sheep-and-goats-across-california
#7
S Sethi, X Chen, P H Kass, B Puschner
It has been previously been shown by our lab and others that persistent organic pollutants, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs), are contaminants in milk produced for human consumption. To further this research we determined the concentration of 21 PCB and 14 PBDE congeners in livestock serum, mainly bovine, across California. Congeners were extracted from serum using solid phase extraction (SPE), cleaned up by silica cartridge and quantified using gas chromatography-triple quadruple mass spectrometry...
April 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28424600/systems-subjects-sessions-to-what-extent-do-these-factors-influence-eeg-data
#8
Andrew Melnik, Petr Legkov, Krzysztof Izdebski, Silke M Kärcher, W David Hairston, Daniel P Ferris, Peter König
Lab-based electroencephalography (EEG) techniques have matured over decades of research and can produce high-quality scientific data. It is often assumed that the specific choice of EEG system has limited impact on the data and does not add variance to the results. However, many low cost and mobile EEG systems are now available, and there is some doubt as to the how EEG data vary across these newer systems. We sought to determine how variance across systems compares to variance across subjects or repeated sessions...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28422699/hemorrhage-prediction-models-in-surgical-intensive-care-bedside-monitoring-data-adds-information-to-lab-values
#9
Marco De Pasquale, Travis Moss, Sergio Cerutti, James Calland, Douglas Lake, Randall Moorman, Manuela Ferrario
Hemorrhage is a frequent complication in surgery patients; its identification and management have received increasing attention as a target for quality improvement in patient care in the Intensive Care Unit (ICU). The purposes of this work were i) to find an early detection model for hemorrhage by exploring the range of data mining methods that are currently available, and ii) to compare prediction models utilizing continuously measured physiological data from bedside monitors to those using commonly obtained laboratory tests...
April 12, 2017: IEEE Journal of Biomedical and Health Informatics
https://www.readbyqxmd.com/read/28420603/the-coags-uncomplicated-app-fulfilling-educational-gaps-around-diagnosis-and-laboratory-testing-of-coagulation-disorders
#10
Craig Kessler, Ellinor I Peerschke, Meera B Chitlur, Roshni Kulkarni, Natalia Holot, David L Cooper
BACKGROUND: Patients with coagulation disorders may present to a variety of physician specialties; however, accurate and efficient diagnosis can be challenging for physicians not specialized in hematology, due to identified gaps in knowledge around appropriate laboratory assays and interpretation of test results. Coags Uncomplicated was developed to fill this unmet educational need by increasing practical knowledge of coagulation disorders among nonexpert physicians and other health care professionals (HCPs) in a point-of-care (POC) setting...
April 18, 2017: JMIR Medical Education
https://www.readbyqxmd.com/read/28419807/otolaryngology-residency-program-research-resources-and-scholarly-productivity
#11
Jennifer A Villwock, Chelsea S Hamill, Brian D Nicholas, Jesse T Ryan
Objective To delineate research resources available to otolaryngology residents and their impact on scholarly productivity. Study Design Survey of current otolaryngology program directors. Setting Otolaryngology residency programs. Subjects and Methods An anonymous web-based survey was sent to 98 allopathic otolaryngology training program directors. Fisher exact tests and nonparametric correlations were used to determine statistically significant differences among various strata of programs. Results Thirty-nine percent (n = 38) of queried programs responded...
April 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28416967/rescuing-ethanol-photosynthetic-production-of-cyanobacteria-in-non-sterilized-outdoor-cultivations-with-a-bicarbonate-based-ph-rising-strategy
#12
Zhi Zhu, Guodong Luan, Xiaoming Tan, Haocui Zhang, Xuefeng Lu
BACKGROUND: Ethanol photosynthetic production based on cyanobacteria cell factories utilizing CO2 and solar energy provides an attractive solution for sustainable production of green fuels. However, the scaling up processes of cyanobacteria cell factories were usually threatened or even devastated by biocontaminations, which restricted biomass or products accumulations of cyanobacteria cells. Thus it is of great significance to develop reliable biocontamination-controlling strategies for promoting ethanol photosynthetic production in large scales...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28411815/automation-of-sample-processing-for-icp-ms-determination-of-90-sr-radionuclide-at-ppq-level-for-nuclear-technology-and-environmental-purposes
#13
Kamila Kołacińska, Ewelina Chajduk, Jakub Dudek, Zbigniew Samczyński, Edyta Łokas, Anna Bojanowska-Czajka, Marek Trojanowicz
(90)Sr is a widely determined radionuclide for environmental purposes, nuclear waste control, and can be also monitored in coolants in nuclear reactor plants. In the developed method, the ICP-MS detection was employed together with sample processing in sequential injection analysis (SIA) setup, equipped with a lab-on-valve with mechanized renewal of sorbent bed for solid-phase extraction. The optimized conditions of determination included preconcentration of (90)Sr on cation-exchange column and removal of different type of interferences using extraction Sr-resin...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28409998/suitability-of-various-dna-extraction-methods-for-a-traditional-chinese-paocai-system
#14
Nan Zhao, Jialiang Cai, Chuchu Zhang, Zhuang Guo, Wenwei Lu, Bo Yang, Feng-Wei Tian, Xiao-Ming Liu, Hao Zhang, Wei Chen
Traditional paocai brine (PB), which is continuously propagated by back-slopping and contains various species of lactic acid bacteria (LAB), is critical for the flavor of paocai. Culture-independent approaches are commonly used to investigate the microbial communities of fermented food. To evaluate the influence of different DNA (DNA) extraction methods on estimates of bacterial community profiles from 4 PBs, the lysis efficiency, DNA yield, purity and denaturing gradient gel electrophoresis (DGGE) profiles of V3 region of a 16S ribosomal ribonucleic acid gene were acquired...
April 14, 2017: Bioengineered
https://www.readbyqxmd.com/read/28406846/the-value-of-simulation-based-training-in-the-path-to-laparoscopic-urological-proficiency
#15
Ben S E P Van Cleynenbreugel, Ali S Gözen, Theodoros Tokas
PURPOSE OF REVIEW: Numerous factors have reduced the training opportunities of surgical residents in the operating room. There is also the question of patient's safety. This gives a need for increased training opportunities outside the operating room. We look if there is an added value in simulation-based training (SBT) in the acquisition of laparoscopic skills. RECENT FINDINGS: Incorporating SBT into the medical students curriculum reduces surgical complication rates in the operating room...
April 12, 2017: Current Opinion in Urology
https://www.readbyqxmd.com/read/28402800/assessment-of-dysglycemia-risk-in-the-kitikmeot-region-of-nunavut-using-the-canrisk-tool
#16
Ying Jiang, Susan Rogers Van Katwyk, Yang Mao, Heather Orpana, Gina Argwal, Margaret de Groh, Monique Skinner, Robyn Clarke, Howard Morrison
INTRODUCTION: The Public Health Agency of Canada adapted a Finnish diabetes screening tool (FINDRISC) to create a tool (CANRISK) tailored to Canada's multi-ethnic population. CANRISK was developed using data collected in seven Canadian provinces. In an effort to extend the applicability of CANRISK to northern territorial populations, we completed a study with the mainly Inuit population in the Kitikmeot region of Nunavut. METHODS: We obtained CANRISK questionnaires, physical measures and blood samples from participants in five Nunavut communities in Kitikmeot...
April 2017: Health Promotion and Chronic Disease Prevention in Canada
https://www.readbyqxmd.com/read/28402051/silage-fermentation-and-ruminal-degradation-of-stylo-prepared-with-lactic-acid-bacteria-and-cellulase
#17
Mao Li, Hanlin Zhou, Xuejuan Zi, Yimin Cai
In order to improve the silage fermentation of stylo (Stylosanthes guianensis) in tropical areas, stylo silages were prepared with commercial additives Lactobacillus plantarum Chikuso-1 (CH1), L. rhamnasus Snow Lact L (SN), Acremonium cellulase (CE) and their combination as SN+CE or CH1 + CE, and the fermentation quality, chemical composition and ruminal degradation of these silages were studied. Stylo silages treated with lactic acid bacteria (LAB) or cellulase, the pH value and NH3 -N ⁄ total-N were significantly (P < 0...
April 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28399996/microbiota-and-metabolome-during-controlled-and-spontaneous-fermentation-of-nocellara-etnea-table-olives
#18
Cinzia Lucia Randazzo, Aldo Todaro, Alessandra Pino, Iole Pitino, Onofrio Corona, Cinzia Caggia
This study is aimed to investigate bacterial community and its dynamics during the fermentation of Nocellara Etnea table olives and to study its effect on metabolome formation. Six different combination of bacterial cultures (BC1-BC6) were used as starters for table olive fermentation and one additional process, conducted without addition of any starters, was used as control (C). The processes were conducted in triplicate and, overall, 21 vessels were performed at industrial scale. The fermentation was monitored for 120 days through culture-dependent and -independent approaches...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399305/energy-usage-of-known-age-blacklegged-ticks-acari-ixodidae-what-is-the-best-method-for-determining-physiological-age
#19
Justin R Pool, Jenna R Petronglo, Richard C Falco, Thomas J Daniels
Ticks expend energy while host-seeking and must consume blood to advance to the next life stage. The energy required for activity is derived from the tick's lipid reserves, a valuable resource that sustains the tick until it finds the next host and can take another bloodmeal. The amount of lipid reserves in an unfed tick has been proposed as an index of tick biological age. Two different methods for aging nymphal blacklegged ticks, Ixodes scapularis Say, were analyzed in this study. To study lipid usage, colony-raised nymphs were held in lab-controlled chambers at 21...
March 14, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28397461/analytical-and-clinical-validation-of-a-point-of-care-cardiac-troponin-t-test-with-an-improved-detection-limit
#20
Carsten Jungbauer, Julian Hupf, Evangelos Giannitsis, Johann Frick, Anna Slagman, Christoph Ehret, Nicolas Herbert, Christine Jung, Rainer Zerback, Thomas Bertsch, Michael Christ
BACKGROUND: The point-of-care test Roche CARDIAC POC Troponin T (PoC TnT) is an improved assay which has been developed for the Roche cobas h 232 system. METHODS: We performed a multicentre evaluation (four sites) to assess the analytical performance of the PoC TnT assay and to compare it with the central laboratory Elecsys® troponin T high sensitive (lab cTnT-hs) assay. RESULTS: The relative mean differences found in method comparisons of PoC TnT vs...
April 1, 2017: Clinical Laboratory
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