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https://www.readbyqxmd.com/read/29149547/engineering-escherichia-coli-co-cultures-for-production-of-curcuminoids-from-glucose
#1
Zhen Fang, J Andrew Jones, Jingwen Zhou, Mattheos A G Koffas
Curcuminoids have attracted increasing attention because of the antioxidant, anticancer, and antitumor activities while their production is limited because of its main source, turmeric plant, demonstrates extensive seasonal variation. In this study, we constructed Escherichia coli co-culture system for the rapid production of curcuminoids from glucose. Firstly, the overexpression of curcuminoid synthase and four different strategies related to increasing the intracellular malonyl-CoA pool were conducted in engineered E...
November 17, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29148844/circadian-phase-circadian-period-and-chronotype-are-reproducible-over-months
#2
Thomas Kantermann, Charmane I Eastman
The timing of the circadian clock, circadian period and chronotype varies among individuals. To date, not much is known about how these parameters vary over time in an individual. We performed an analysis of the following five common circadian clock and chronotype measures: 1) the dim light melatonin onset (DLMO, a measure of circadian phase), 2) phase angle of entrainment (the phase the circadian clock assumes within the 24-h day, measured here as the interval between DLMO and bedtime/dark onset), 3) free-running circadian period (tau) from an ultradian forced desynchrony protocol (tau influences circadian phase and phase angle of entrainment), 4) mid-sleep on work-free days (MSF from the Munich ChronoType Questionnaire; MCTQ) and 5) the score from the Morningness-Eveningness Questionnaire (MEQ)...
November 17, 2017: Chronobiology International
https://www.readbyqxmd.com/read/29146249/composition-of-lactic-acid-bacteria-during-spontaneous-curly-kale-brassica-oleracea-var-sabellica-fermentation
#3
Magdalena Michalak, Klaudia Gustaw, Adam Waśko, Magdalena Polak-Berecka
The present work is the first report on spontaneous fermentation of curly kale and characteristics of autochthonous lactic acid bacteria (LAB). Our results indicate that curly kale fermentation is the new possibility of the technological use of this vegetable. Bacteria representing ten different species were isolated from three phases of curly kale fermentation and identified by MALDI-TOF mass spectrometry and 16S rRNA gene sequencing. Among them, four species were identified as Lactobacillus spp. (Lb. plantarum 332, Lb...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29137079/treatment-of-hypermyoglobinemia-after-crs-hipec-for-patients-with-peritoneal-carcinomatosis-a-retrospective-comparative-study
#4
Gang Liu, Zhong-He Ji, Yang Yu, Xin-Bao Li, Yan-Bin Zhang, Kai-Wen Peng, Yan Li
This retrospective comparative study aims to explore the time courses of serum myoglobin (Mb) changes, and summarize our experience in treating patients with hypermyoglobinemia after cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC).This study covered 60 patients with peritoneal carcinomatosis treated with CRS + HIPEC as the study group, and another 25 cancer patients treated with conventional extensive surgery without HIPEC as the control group from February to October 2016...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29136919/flow-batch-analysis-of-clenbuterol-based-on-analyte-extraction-on-molecularly-imprinted-polymers-coupled-to-an-in-system-chromogenic-reaction-application-to-human-urine-and-milk-substitute-samples
#5
Natalia González, Marcos Grünhut, Ivana Šrámková, Adriana G Lista, Burkhard Horstkotte, Petr Solich, Hana Sklenářová, Carolina C Acebal
A fully automated spectrophotometric method based on flow-batch analysis has been developed for the determination of clenbuterol including an on-line solid phase extraction using a molecularly imprinted polymer (MIP) as the sorbent. The molecularly imprinted solid phase extraction (MISPE) procedure allowed analyte extraction from complex matrices at low concentration levels and with high selectivity towards the analyte. The MISPE procedure was performed using a commercial MIP cartridge that was introduced into a guard column holder and integrated in the analyzer system...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136838/a-robotic-magnetic-nanoparticle-solid-phase-extraction-system-coupled-to-flow-batch-analyzer-and-gfaas-for-determination-of-trace-cadmium-in-edible-oils-without-external-pretreatment
#6
Inakã S Barreto, Stefani I E Andrade, Francisco A S Cunha, Marcelo B Lima, Mario Cesar U Araujo, Luciano F Almeida
A lab-made magnetic-mechanical robotic (MMR) system coupled to a flow-batch analyzer (FBA) for magnetic nanoparticles solid phase extraction (MSPE) is presented. As an illustrative application, an NMR-FBA couple was connected to a graphite furnace atomic absorption spectrometer (GFAAS) for quantification of trace cadmium in edible oils. Factors affecting MSPE, such as the amount of adsorbent, the type, concentration and volume of the eluent and elution time were studied. Under the optimized experimental conditions, the interferents studied did not reveal a significant change in the analytical response, indicating that proposed method is selective...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29130876/efficacy-of-arthroscopic-teaching-methods-a-prospective-randomized-controlled-study
#7
Luke Robinson, Jonathon Spanyer, Zachary Yenna, Patrick Burchell, Andrew Garber, John Riehl
Arthroscopic education research recently has been focused on the use of skills labs to facilitate resident education and objective measure development to gauge technical skill. This study evaluates the effectiveness of three different teaching methods. Medical students were randomized into three groups. The first group received only classroom-based lecture. The second group received the same lecture and 28 minutes of lab-based hands-off arthroscopy instruction using a cadaver and arthroscopy setup. The final group received the same lecture and 7 minutes of hands-on arthroscopy instruction in the lab on a cadaver knee...
October 2017: Journal of Surgical Orthopaedic Advances
https://www.readbyqxmd.com/read/29116200/internal-extractive-electrospray-ionization-mass-spectrometry-for-quantitative-determination-of-fluoroquinolones-captured-by-magnetic-molecularly-imprinted-polymers-from-raw-milk
#8
Hua Zhang, Wei Kou, Aisha Bibi, Qiong Jia, Rui Su, Huanwen Chen, Keke Huang
Antibiotics contamination in food products is of increasing concern due to their potential threat on human health. Herein solid-phase extraction based on magnetic molecularly imprinted polymers coupled with internal extractive electrospray ionization mass spectrometry (MMIPs-SPE-iEESI-MS) was designed for the quantitative analysis of trace fluoroquinolones (FQs) in raw milk samples. FQs in the raw milk sample (2 mL) were selectively captured by the easily-lab-made magnetic molecularly imprinted polymers (MMIPs), and then directly eluted by 100 µL electrospraying solvent biased with +3...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29100375/mlacp-machine-learning-based-prediction-of-anticancer-peptides
#9
Balachandran Manavalan, Shaherin Basith, Tae Hwan Shin, Sun Choi, Myeong Ok Kim, Gwang Lee
Cancer is the second leading cause of death globally, and use of therapeutic peptides to target and kill cancer cells has received considerable attention in recent years. Identification of anticancer peptides (ACPs) through wet-lab experimentation is expensive and often time consuming; therefore, development of an efficient computational method is essential to identify potential ACP candidates prior to in vitro experimentation. In this study, we developed support vector machine- and random forest-based machine-learning methods for the prediction of ACPs using the features calculated from the amino acid sequence, including amino acid composition, dipeptide composition, atomic composition, and physicochemical properties...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29099324/graf3-serves-as-a-blood-volume-sensitive-rheostat-to-control-smooth-muscle-contractility-and-blood-pressure
#10
Xue Bai, Kevin Mangum, Masao Kakoki, Oliver Smithies, Christopher P Mack, Joan M Taylor
Vascular resistance is a major determinant of BP and is controlled, in large part, by RhoA-dependent smooth muscle cell (SMC) contraction within small peripheral arterioles and previous studies from our lab indicate that GRAF3 is a critical regulator of RhoA in vascular SMC. The elevated contractile responses we observed in GRAF3 deficient vessels coupled with the hypertensive phenotype provided a mechanistic link for the hypertensive locus recently identified within the GRAF3 gene. On the basis of our previous findings that the RhoA signaling axis also controls SMC contractile gene expression and that GRAF3 expression was itself controlled by this pathway, we postulated that GRAF3 serves as an important counter-regulator of SMC phenotype...
November 3, 2017: Small GTPases
https://www.readbyqxmd.com/read/29095940/expressiondb-an-open-source-platform-for-distributing-genome-scale-datasets
#11
Laura D Hughes, Scott A Lewis, Michael E Hughes
RNA-sequencing (RNA-seq) and microarrays are methods for measuring gene expression across the entire transcriptome. Recent advances have made these techniques practical and affordable for essentially any laboratory with experience in molecular biology. A variety of computational methods have been developed to decrease the amount of bioinformatics expertise necessary to analyze these data. Nevertheless, many barriers persist which discourage new labs from using functional genomics approaches. Since high-quality gene expression studies have enduring value as resources to the entire research community, it is of particular importance that small labs have the capacity to share their analyzed datasets with the research community...
2017: PloS One
https://www.readbyqxmd.com/read/29090435/effects-of-storage-temperature-and-time-of-antimony-release-from-pet-bottles-into-drinking-water-in-china
#12
Fei Qiao, Kun Lei, Zicheng Li, Qing Liu, Zhanliang Wei, Lihui An, Hongli Qi, Song Cui
Antimony (Sb) concentrations were measured in 10 brands of PET bottled drinking water available in supermarkets in China. To simulate general storage habits based on market research, these PET bottles with drinking water were stored for 4 weeks in a lab or a car trunk during the summer. Although the PET package material of brand A had the lowest Sb level (142.71 ± 29.81 μg/g), it showed a significant increase in Sb concentrations when stored in both the car trunk and the lab. There was significant release of Sb from the PET bottles into the water following 24 h of incubation at ≥ 40 °C (40, 50, 60, and 70 °C), especially at 70 °C...
October 31, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29083976/letter-corrected-net-health-benefit-calculations-for-enzalutamide-using-asco-value-framework-guidelines-and-nccn-evidence-blocks
#13
Scott Flanders, Bruce Brown, Marjan Massoudi, Neil Schultz, Krishnan Ramaswamy
This research was funded by Astellas Pharma and Medivation, which was acquired by Pfizer in 2016. Astellas Pharma and Medivation are the co-developers of enzalutamide. Flanders, Brown, Massoudi, and Schultz are employees of Astellas Pharma. Ramaswamy is an employee of Pfizer and holds stock in Johnson & Johnson. Flanders holds stock in Johnson & Johnson, AbbVie, and Abbott Labs.
November 2017: Journal of Managed Care & Specialty Pharmacy
https://www.readbyqxmd.com/read/29083113/hydroxyethyl-starch-and-kidney-function-a-retrospective-study-in-patients-undergoing-therapeutic-plasma-exchange
#14
Muthuchellappan Radhakrishnan, Akshay Batra, Sundar Periyavan, Mariamma Philip, Vivek Anand
PURPOSE: Hydroxyethyl starch (HES) and albumin are used as replacement fluids during therapeutic plasma exchange (TPE). HES solutions are no longer recommended in critically ill patients due to its effect on kidneys and coagulation. In this retrospective study, we tried to look at the association between cumulative HES administration and kidney function in patients undergoing TPE. METHODS: Transfusion medicine department register was scrutinized to identify adult patients who had completed at least 5 cycles of TPE during the period June 2014-May 2015...
October 30, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29082061/forty-three-year-old-female-with-dopamine-secreting-pheochromocytoma-of-the-adrenal-gland
#15
Tyler Haden, Marcin Zuberek, Naveen Pokala
We report on a 43-year-old, asymptomatic female who presented with incidental finding of left adrenal mass. MRI gave concerns for possible pheochromocytoma but markers for pheochromocytoma were not elevated as expected. 24-hour urine dopamine levels (6988 μg/day) were significantly elevated. The patient successfully underwent robotic assisted radical left adrenalectomy and was diagnosed with a dopamine secreting pheochromocytoma. Pathology revealed increased malignant potential associated with the tumor...
2017: Case Reports in Urology
https://www.readbyqxmd.com/read/29065485/a-wearable-magneto-inertial-system-for-gait-analysis-h-gait-validation-on-normal-weight-and-overweight-obese-young-healthy-adults
#16
Valentina Agostini, Laura Gastaldi, Valeria Rosso, Marco Knaflitz, Shigeru Tadano
Background: Wearable magneto-inertial sensors are being increasingly used to obtain human motion measurements out of the lab, although their performance in applications requiring high accuracy, such as gait analysis, are still a subject of debate. The aim of this work was to validate a gait analysis system (H-Gait) based on magneto-inertial sensors, both in normal weight (NW) and overweight/obese (OW) subjects. The validation is performed against a reference multichannel recording system (STEP32), providing direct measurements of gait timings (through foot-switches) and joint angles in the sagittal plane (through electrogoniometers)...
October 21, 2017: Sensors
https://www.readbyqxmd.com/read/29064772/reducing-diabetes-distress-and-improving-self-management-with-mindfulness
#17
Robin R Whitebird, Mary Jo Kreitzer, Gabriela Vazquez-Benitez, Chris J Enstad
Stress associated with diabetes makes managing diabetes harder. We investigated whether mindfulness-based stress reduction (MBSR) could reduce diabetes distress and improve management. We recruited 38 participants to complete an MBSR program. Surveys and lab values were completed at baseline and post-intervention. Participants showed significant improvement in diabetes-related distress (Cohen's d -.71, p < .002), psychosocial self-efficacy (Cohen's d .80, p < .001), and glucose control (Cohen's d -...
October 24, 2017: Social Work in Health Care
https://www.readbyqxmd.com/read/29060835/phonequant-a-smartphone-based-quantitative-immunoassay-analyser
#18
Malay Ilesh Shah, Jayaraj Joseph, Ujwal Sriharsha Sanne, Mohanasankar Sivaprakasam
There is a vital need for portable and cost-effective point-of-care (PoC) testing technologies that provide reliable and rapid results. Lateral Flow Immunoassays (LFIA) are suitable PoC diagnostic tools with the potential for use in a wide variety of field applications ranging from uses in clinical diagnostics to aiding law enforcement. Quick and reliable diagnosis of non-communicable diseases (NCD) like diabetes is vital especially in developing countries like India where the burden of these diseases is very high and is increasing day by day...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29058595/world-wide-immunoscore-task-force-meeting-report-from-the-melanoma-bridge-napoli-november-30th-december-3rd-2016
#19
Jerome Galon, Alessandro Lugli, Carlo Bifulco, Franck Pages, Giuseppe Masucci, Francesco M Marincola, Paolo A Ascierto
The predictive accuracy of the traditional staging system is based on disease progression as a tumour cell-autonomous process, but it fails to incorporate the effects of the host immune response. A precise analysis of the immune component of the tumour microenvironment by computer-based analysis may be essential to managing patients better, opening the road to an expertise in this new emerging field. The Immunoscore as a new possible approach in the classification of cancer, designated TNM-Immune, studied in colon cancer patients with predictive and prognostic value...
October 20, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29056336/the-impact-of-age-and-comorbidities-on-practice-patterns-and-outcomes-in-patients-with-relapsed-refractory-multiple-myeloma-in-the-era-of-novel-therapies
#20
Parameswaran Hari, Dorothy Romanus, Katarina Luptakova, Marlo Blazer, Candice Yong, Aditya Raju, Eileen Farrelly, Richard Labotka, Vicki A Morrison
OBJECTIVES: One-third of patients with multiple myeloma (MM) are diagnosed at age≥75years. Older patients have increased incidence of cardiovascular disease (CVD) and renal insufficiency (RI), hallmark complications of MM. We examined cumulative incidence of CVD and RI in relapsed/refractory MM (RRMM) and outcomes by age and RI/CVD. MATERIALS AND METHODS: Retrospective cohort study using a large US electronic medical records database of adult patients with RRMM initiating first- and second-line therapy (2LT) between 1/2008-06/2015...
October 19, 2017: Journal of Geriatric Oncology
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