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https://www.readbyqxmd.com/read/29775547/acute-dietary-nitrate-supplementation-does-not-attenuate-oxidative-stress-or-the-hemodynamic-response-during-submaximal-exercise-in-hypobaric-hypoxia
#1
Colin R Carriker, Paige Rombach, Brooke M Stevens, Roger A Vaughan, Ann L Gibson
PURPOSE: To investigate changes in oxidative stress, arterial oxygen saturation (SaO2), blood pressure (BP), and heart rate (HR) while exercising in hypobaric hypoxia following acute dietary nitrate supplementation. METHODS: Nine well-trained (60.8±7.8 ml/kg/min) males (29±7 years) visited the lab on 3 occasions, each separated by 1 week. Visit 1 included a maximal aerobic cycling test (VO2max) and 5 five-minute increasing intensity exercise bouts in a normobaric environment (1600m)...
May 18, 2018: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/29774394/longitudinal-health-care-cost-in-hypoplastic-left-heart-syndrome-palliation
#2
Jesse E Hansen, Nicolas L Madsen, Laurie Bishop, David L S Morales, Jeffrey B Anderson
Management of hypoplastic left heart syndrome (HLHS) is resource intensive. Heath care systems are pressured to provide value to patients by improving outcomes while decreasing costs. A single-center retrospective cohort of infants with HLHS who underwent Norwood procedure or hybrid Norwood from 2004 to 2014 and survived to first outpatient follow up were studied. The primary outcome was total cost through 12 months with a sub-analysis of patients with 60 months of data. Costs were calculated using internal cost accounting system and reported by cost center...
May 17, 2018: Pediatric Cardiology
https://www.readbyqxmd.com/read/29771522/hydronium-ions-accompanying-buried-acidic-residues-lead-to-high-apparent-dielectric-constants-in-the-interior-of-proteins
#3
Xiongwu Wu, Bernard R Brooks
Internal ionizable groups are known to play important roles in protein functions. A mystery that has attracted decades of extensive experimental and theoretical studies is the apparent dielectric constants experienced by buried ionizable groups, which are much higher than values expected for protein interiors. Many interpretations have been proposed, such as water penetration, conformational relaxation, local unfolding, protein intrinsic backbone fluctuations, etc. However, these interpretations conflict with many experimental observations...
May 17, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29769498/quantitative-analysis-of-nucleic-acid-extraction-methods-for-vibrio-cholerae-using-real-time-pcr-and-conventional-pcr
#4
Z Z Hossain, J Ferdous, S M Tulsiani, P M Jensen, A Begum
The devastating diarrheal disease cholera is caused by the bacterium Vibrio cholerae. Nucleic acid extraction is the primary step for several molecular detection approaches. In order to identify the sources of cholera illness, an efficient, fast and easy DNA extraction method for toxigenic Vibrio cholerae is desired which can be applied on diverse type of samples. This methodology developmental setup study was performed in the Environmental Microbiology Lab, Department of Microbiology, University of Dhaka, Bangladesh from March 2015 to May 2015 attempted to compare three DNA extraction methods for efficient detection and quantification of Vibrio cholerae...
April 2018: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/29767357/a-guide-for-designing-and-analyzing-rna-seq-data
#5
Aniruddha Chatterjee, Antonio Ahn, Euan J Rodger, Peter A Stockwell, Michael R Eccles
The identity of a cell or an organism is at least in part defined by its gene expression and therefore analyzing gene expression remains one of the most frequently performed experimental techniques in molecular biology. The development of the RNA-Sequencing (RNA-Seq) method allows an unprecedented opportunity to analyze expression of protein-coding, noncoding RNA and also de novo transcript assembly of a new species or organism. However, the planning and design of RNA-Seq experiments has important implications for addressing the desired biological question and maximizing the value of the data obtained...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29766202/photochemical-device-for-selective-detection-of-phenol-in-aqueous-solutions
#6
Nianbing Zhong, Ming Chen, Zhengkun Wang, Xin Xin, Bingxin Li
We demonstrate that a lab-on-a-chip device (hereafter termed a photochemical phenol sensor) that integrates a photocatalytic long-period fiber grating (PLPFG), fiber Bragg grating (FBG), polymer membrane, ultraviolet (UV) visible light, and microchannels can be exploited to selectively detect phenol in aqueous solutions. The novel PLPFG consisted of a thinned long-period fiber grating (LPFG) and a UV-visible-light-driven Er3+:YAlO3/SiO2/TiO2 (EYST) coating. The polymer membrane with high phenol permselectivity was synthesized using PEBA2533 doped with β-cyclodextrin and was wrapped around the EYST surface, thus forming a microchannel between the membrane and PLPFG to enable the injection and outflow of standard analytes...
May 16, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29766137/after-the-embo-predicting-non-hemorrhagic-indications-for-splenectomy-after-angioembolization-in-patients-with-blunt-trauma
#7
John F Bilello, Victoria L Sharp, Rachel C Dirks, Krista L Kaups, James W Davis
Background: Successful non-operative management (NOM) of blunt splenic trauma is enhanced with splenic angioembolization (SAE). Patients may still require splenectomy post-SAE for splenic infarction/necrosis. Prior studies have used white blood cell count (WBC), platelet count (PLT), and PLT:WBC ratio after splenectomy to predict complications, but none have evaluated these findings prior to splenectomy in patients who have undergone SAE. Changes in these values may indicate clinically significant splenic infarction, facilitating management of these patients...
2018: Trauma surgery & acute care open
https://www.readbyqxmd.com/read/29765654/membrane-assisted-extraction-of-monoterpenes-from-in-silico-solvent-screening-towards-biotechnological-process-application
#8
L Janoschek, L Grozdev, S Berensmeier
This work focuses on the process development of membrane-assisted solvent extraction of hydrophobic compounds such as monoterpenes. Beginning with the choice of suitable solvents, quantum chemical calculations with the simulation tool COSMO-RS were carried out to predict the partition coefficient (log P ) of (S)-(+)-carvone and terpinen-4-ol in various solvent-water systems and validated afterwards with experimental data. COSMO-RS results show good prediction accuracy for non-polar solvents such as n-hexane, ethyl acetate and n-heptane even in the presence of salts and glycerol in an aqueous medium...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29761520/predicting-lysine-malonylation-sites-of-proteins-using-sequence-and-predicted-structural-features
#9
Ghazaleh Taherzadeh, Yuedong Yang, Haodong Xu, Yu Xue, Alan Wee-Chung Liew, Yaoqi Zhou
Malonylation is a recently discovered post-translational modification (PTM) in which a malonyl group attaches to a lysine (K) amino acid residue of a protein. In this work, a novel machine learning model, SPRINT-Mal, is developed to predict malonylation sites by employing sequence and predicted structural features. Evolutionary information and physicochemical properties are found to be the two most discriminative features whereas a structural feature called half-sphere exposure provides additional improvement to the prediction performance...
May 14, 2018: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/29757709/how-to-assess-the-best-immunohistochemical-panel-in-the-diagnosis-of-malignant-pleural-mesothelioma-in-a-pathology-lab
#10
Mona Mlika, Oumeima Lamzibri, Saoussen Bacha, Soumeya Laabidi, Chokri Haddouchi, Faouzi El Mezni
BACKGROUND: malignant pleural mesothelioma (MPM) is a rare tumor with a challenging diagnosis. Even if, clinical data are mandatory to suspect the diagnosis, the positive diagnosis is based on microscopic features. Morphologic features are still the port of call of the diagnosis but their non specific character and the multiplicity of differential diagnoses made the immunohistochemical markers mandatory for the diagnosis. Many antibodies with a positive diagnostic value including claretinin, mesothelin, WT1 and antibodies with a negative diagnostic value including TTF1, EMA, CD15 are recommended by the scientific societies...
May 14, 2018: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29756858/first-monte-carlo-global-analysis-of-nucleon-transversity-with-lattice-qcd-constraints
#11
H-W Lin, W Melnitchouk, A Prokudin, N Sato, H Shows
We report on the first global QCD analysis of the quark transversity distributions in the nucleon from semi-inclusive deep-inelastic scattering (SIDIS), using a new Monte Carlo method based on nested sampling and constraints on the isovector tensor charge g_{T} from lattice QCD. A simultaneous fit to the available SIDIS Collins asymmetry data is compatible with g_{T} values extracted from a comprehensive reanalysis of existing lattice simulations, in contrast to previous analyses, which found significantly smaller g_{T} values...
April 13, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/29750250/analysis-of-fentanyl-and-18-novel-fentanyl-analogs-and-metabolites-by-lc-ms-ms-and-report-of-fatalities-associated-with-methoxyacetylfentanyl-and-cyclopropylfentanyl
#12
Melissa F Fogarty, Donna M Papsun, Barry K Logan
Methoxyacetylfentanyl and cyclopropylfentanyl are two of the newest illicit opioids that are infiltrating the heroin market. Methoxyacetylfentanyl and cyclopropylfentanyl were reported by the Drug Enforcement Administration (DEA) in their third quarter report of 2017 to have been chemically identified seven and five times, respectively, from drug evidence analyzed by the DEA's lab system; Q3 was the first time cyclopropylfentanyl was identified by the DEA's lab system, while methoxyacetylfentanyl was reported one time in Q2 2017...
May 10, 2018: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/29749844/teaching-assistant-attention-and-responsiveness-to-student-reasoning-in-written-work
#13
Cynthia F C Hill, Julia S Gouvea, David Hammer
Instructors communicate what they value about students' written work through their comments and feedback, and this feedback has the potential to direct how students approach writing assignments. In this study, we examined how graduate student teaching assistants (TAs) attended and responded to students' written lab reports in an introductory biology course. We collected and analyzed marked lab reports from five TAs and interviewed them about their marking decisions. The results show that TAs attended mainly to writing style and form in their markings and comments on lab reports...
June 2018: CBE Life Sciences Education
https://www.readbyqxmd.com/read/29744918/retrospective-analysis-of-rf-heating-measurements-of-passive-medical-implants
#14
Ting Song, Zhiheng Xu, Maria Ida Iacono, Leonardo M Angelone, Sunder Rajan
PURPOSE: The test reports for the RF-induced heating of metallic devices of hundreds of medical implants have been provided to the U.S. Food and Drug Administration as a part of premarket submissions. The main purpose of this study is to perform a retrospective analysis of the RF-induced heating data provided in the reports to analyze the trends and correlate them with implant geometric characteristics. METHODS: The ASTM-based RF heating test reports from 86 premarket U...
May 9, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29743692/precision-measurement-of-the-weak-charge-of-the-proton
#15
(no author information available yet)
Large experimental programmes in the fields of nuclear and particle physics search for evidence of physics beyond that explained by current theories. The observation of the Higgs boson completed the set of particles predicted by the standard model, which currently provides the best description of fundamental particles and forces. However, this theory's limitations include a failure to predict fundamental parameters, such as the mass of the Higgs boson, and the inability to account for dark matter and energy, gravity, and the matter-antimatter asymmetry in the Universe, among other phenomena...
May 2018: Nature
https://www.readbyqxmd.com/read/29742232/can-low-fusing-glass-application-affect-the-marginal-misfit-and-bond-strength-of-y-tzp-crowns
#16
Monize Carelli Felipe Antunes, Jean Soares Miranda, Ronaldo Luís Almeida de Carvalho, Rodrigo Furtado de Carvalho, Estevão Tomomitsu Kimpara, Rodrigo Othávio de Assunção E Souza, Fabíola Pessôa Pereira Leite
To evaluate the effect of different surface treatments on the marginal misfit and retentive strength between Y-TZP crowns and an epoxy resin. Forty (40) epoxy resin (G10) abutments (height: 5mm, conicity: 60, finish line: large chamfer) with equal dimensions were milled and included in polyurethane to simulate the periodontal ligament. Next, 40 Y-TZP crowns (thickness: 1mm) were milled (Cerec in Lab) and randomly divided into four groups (n=10) according to the surface treatment: GS(glaze spray), GP(glaze powder/liquid), P(zirconia primer) and RS(tribochemical silica coating)...
2018: Brazilian Oral Research
https://www.readbyqxmd.com/read/29737394/effects-of-natural-deep-eutectic-solvents-on-lactic-acid-bacteria-viability-during-cryopreservation
#17
Ying Qiao, Hao-Liang Cai, Xi Yang, Yuan-Yuan Zang, Zhi-Gang Chen
In this study, a new method of cryopreservation of lactic acid bacteria (LAB) using natural deep eutectic solvents (NADESs) was developed. Survival rates of LAB during 24-h short-term and 180-day long-term cryostorage at - 20 °C were investigated. The results revealed that survival of Streptococcus thermophilus (S. thermophilus) in NADESs after 24 h of cryostorage was superior to survival of two other tested LAB. Moreover, survival was higher at a ratio of NADESs to S. thermophilus of 1:1 (v/v) than observed using a 4:1 (v/v) ratio...
May 8, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29735046/exploiting-antagonistic-activity-of-fruit-derived-lactobacillus-to-control-pathogenic-bacteria-in-fresh-cheese-and-chicken-meat
#18
Whyara Karoline Almeida da Costa, Geany Targino de Souza, Larissa Ramalho Brandão, Rafael Cardoso de Lima, Estefânia Fernandes Garcia, Marcos Dos Santos Lima, Evandro Leite de Souza, Maria Saarela, Marciane Magnani
This study assessed the antagonistic activity of fruit-derived lactic acid bacteria (LAB) strains against food-related bacteria and the effects of the highest organic acids LAB producers on the survival of Listeria monocytogenes and Salmonella Enteritidis PT4 in cheese and chicken meat, respectively. The production of organic acids by the Lactobacillus strains in the tested food matrices was also monitored. All tested LAB strains showed antagonistic activity in vitro on the growth of pathogenic or spoiling food-related bacteria, particularly on L...
June 2018: Food Research International
https://www.readbyqxmd.com/read/29734815/dynamic-field-testing-of-coating-chemistry-candidates-by-a-rotating-disk-system
#19
Kim A Nolte, Julian Koc, J M Barros, Kelli Hunsucker, Michael P Schultz, G E Swain, Axel Rosenhahn
Quick and reliable testing is crucial for the development of new fouling release (FR) coatings. Exposure of these coatings to natural multispecies communities is essential in evaluating their efficacy. To this end, we present a rotating disk setup for dynamic field exposure. To achieve a well-defined flow on the surface of the disk, an easy to use sample mounting system was developed that provides a smooth and even surface. We related the angular velocity of the disk to the wall shear stress on the surface with a hydrodynamic model...
May 8, 2018: Biofouling
https://www.readbyqxmd.com/read/29734082/biotoxicity-assessment-and-toxicity-mechanism-on-coal-gasification-wastewater-cgw-a-comparative-analysis-of-effluent-from-different-treatment-processes
#20
Weiwei Ma, Yuxing Han, Chunyan Xu, Hongjun Han, Hao Zhu, Kun Li, Mengqi Zheng
Even though coal gasification wastewater (CGW) treated by various biochemical treatment processes generally met the national discharge standard, its potential biotoxicity was still unknown. Therefore, in this study, bioassay with Tetrahymena thermophila (T. thermophila) was conducted to comprehensively evaluate the variation of biotoxicity in raw CGW and the treated effluent from lab-scale micro-electrolysis integrated with biological reactor (MEBR), single iron-carbon micro-electrolysis (ICME) and conventional activated sludge (CAS) processes...
May 4, 2018: Science of the Total Environment
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