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https://www.readbyqxmd.com/read/29166725/-effect-and-related-mechanism-of-mirna-21-on-hydrogen-peroxide-induced-c-kit-cardiac-stem-cells-apoptosis
#1
R Z Zhao, Y Wang, W W Deng, B Shi, X P Long, Z L Wang, W M Chen
Objective: To explored the effect and related mechanism of miRNA-21 on hydrogen peroxide-induced C-kit(+) cardiac stem cells apoptosis. Methods: C-kit(+) cardiac stem cells were isolated from SD rats by the methods of enzyme digestion and magnetic bead. Cells were divided into the following experimental groups: (1) negative control mimics (NCM)group: cells were transfected with negative control miRNA-21 mimics for 48 hours; (2)mimics group: cells were transfected with miRNA-21 mimics for 48 hours; (3) NCM+ H(2)O(2) group: negative control miRNA-21 mimics were transfected into cells for 48 hours and then treated with 100 μmol H(2)O(2) for 2 hours; (4)mimics+ H(2)O(2) group: miRNA-21 mimics were transfected into cells for 48 hours and then treated with 100 μmol H(2)O(2) for 2 hours...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29166723/-association-between-serum-n%C3%AE%C2%B5-carboxymethyl-lysine-level-and-anterior-tibial-arterial-plaque-calcification-in-patients-with-diabetic-foot-post-foot-amputation
#2
L H Li, F Ye, X L Fu, S N Xu, Z Y Bao, Z Sun, J C Yan, J N Wu, Z Q Wang
Objective: To observe the correlation between Nε-carboxymethyl-Lysine (CML), the main component of advanced glycation end products and the calcification of the anterior tibial artery plaque in patients with diabetic foot post foot amputation. Methods: Sixty patients hospitalized for foot amputation operation due to diabetic foot from June 2012 to June 2016 in the Department of Orthopedics, Affiliated Hospital of Jiangsu University were prospectively recruited.The patients were categorized into mild stenosis (0<stenosis<50%, n=20), moderate stenosis (50%≤stenosis<70%, n=20) and severe stenosis (70%≤stenosis≤100%, n=20) based on the color Doppler ultrasound assessed severity of anterior tibial artery stenosis...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29166688/-perioperative-management-in-total-knee-arthroplasty
#3
Robert Hube, Hermann O Mayr, Philipp von Roth, Michael Najfeld, Kathi Thiele
The themes of this paper are the clinical relevance and technique of perioperative management in total knee arthroplasty. Due to the number of unsatisfied patients after knee arthroplasty, optimisation of processes seems to be necessary. Beside patient preparation and rehabilitation, the surgical technique and implant choice in perioperative management are important. This means the use of tourniquet and drains, the application of tranexamic acid and pain management. These items are presented and discussed in detail in this paper...
November 22, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29166664/is-there-a-reasonable-excuse-for-not-providing-post-operative-analgesia-when-using-animal-models-of-peripheral-neuropathic-pain-for-research-purposes
#4
Sara Hestehave, Gordon Munro, Rie Christensen, Tina Brønnum Pedersen, Lars Arvastson, Philip Hougaard, Klas S P Abelson
INTRODUCTION: The induction of neuropathic pain-like behaviors in rodents often requires surgical intervention. This engages acute nociceptive signaling events that contribute to pain and stress post-operatively that from a welfare perspective demands peri-operative analgesic treatment. However, a large number of researchers avoid providing such care based largely on anecdotal opinions that it might interfere with model pathophysiology in the longer term. OBJECTIVES: To investigate effects of various peri-operative analgesic regimens encapsulating different mechanisms and duration of action, on the development of post-operative stress/welfare and pain-like behaviors in the Spared Nerve Injury (SNI)-model of neuropathic pain...
2017: PloS One
https://www.readbyqxmd.com/read/29166659/sex-specific-influences-of-mtdna-mitotype-and-diet-on-mitochondrial-functions-and-physiological-traits-in-drosophila-melanogaster
#5
Wen C Aw, Michael R Garvin, Richard G Melvin, J William O Ballard
Here we determine the sex-specific influence of mtDNA type (mitotype) and diet on mitochondrial functions and physiology in two Drosophila melanogaster lines. In many species, males and females differ in aspects of their energy production. These sex-specific influences may be caused by differences in evolutionary history and physiological functions. We predicted the influence of mtDNA mutations should be stronger in males than females as a result of the organelle's maternal mode of inheritance in the majority of metazoans...
2017: PloS One
https://www.readbyqxmd.com/read/29166575/chemical-characterization-and-pharmacological-assessment-of-cashew-gum-extract-anacardium-occidentale-l-polysaccharide-free
#6
Daiany Priscilla Bueno da Silva, Iziara Ferreira Florentino, Lorrane Kelle da Silva Moreira, Adriane Ferreira Brito, Verônica Vale Carvalho, Géssica Adriana Vasconcelos, Boniek Gontijo Vaz, Marcus Antônio Pereira-Junior, Kátia Flávia Fernandes, Elson Alves Costa
ETHNOPHARMACOLOGICAL RELEVANCE: The cashew gum (Anacardium occidentale L.) is used in Brazilian traditional medicine in the treatment of inflammatory conditions, asthma, diabetes, and gastrointestinal disturbs. AIM OF THE STUDY: The present study was aimed the chemical characterization and the investigation of the antinociceptive and anti-inflammatory activities of cashew gum aqueous extract without the presence of polysaccharides in its composition (CGE). MATERIALS AND METHODS: The CGE was obtained after precipitation and removal of polysaccharides using acetone...
November 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29166547/chemical-metabolome-assay-by-high-resolution-orbitrap-mass-spectrometry-and-assessment-of-associated-antitumoral-activity-of-actinocephalus-divaricatus
#7
Ana C Zanatta, Angela Mari, Milena Masullo, Iracilda Zeppone Carlos, Wagner Vilegas, Sonia Piacente, Lourdes Campaner Dos Santos
RATIONALE: Actinocephalus divaricatus (Eriocaulaceae) is an important source of income for the rural communities as it is sold as an ornamental plant. To date, no investigation has been conducted concerning the chemical composition and biological studies of the aerial parts of A. divaricatus. METHODS: The methanolic extract of the aerial parts of this species was chemically characterized. We applied an analytical dereplication approach based on Liquid Chromatography coupled to High Resolution Orbitrap Mass Spectrometry in order to develop, identify and define rapidly the metabolite fingerprint of the aerial parts of A...
November 22, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29166540/statistical-optimization-of-an-rp-hplc-method-for-the-determination-of-selected-flavonoids-in-berry-juices-and-evaluation-of-their-antioxidant-activities
#8
Andrija Ciric, Milena Jelikic-Stankov, Milica Cvijovic, Predrag Djurdjevic
An isocratic RP-HPLC method for the separation and identification of selected flavonoids (quercetin, rutin, luteolin-7-O-glucoside, kaempferol, and kaempferol-3-O-glucoside) in commercial berries juices (blackcurrant, blueberry, red raspberry and cherry) was developed with the aid of central composite design and response surface methodology. The optimal separation conditions were a mobile phase of 85:15 (% v/v) water/acetonitrile, pH of 2.8 (adjusted with formic acid), flow rate 0.5 mL/min and column temperature of 35 °C...
November 22, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29166530/new-azhdarchoid-pterosaur-pterosauria-pterodactyloidea-with-an-unusual-lower-jaw-from-the-portezuelo-formation-upper-cretaceous-neuqu%C3%A3-n-group-patagonia-argentina
#9
Alexander W A Kellner, Jorge O Calvo
A new azhdarchoid pterosaur from the Upper Cretaceous of Patagonia is described. The material consists of an incomplete edentulous lower jaw that was collected from the upper portion of the Portezuelo Formation (Turonian-Early Coniacian) at the Futalognko site, northwest of Neuquén city, Argentina. The overall morphology of Argentinadraco barrealensis gen. et sp. nov. indicates that it belongs to the Azhdarchoidea and probable represents an azhdarchid species. The occlusal surface of the anterior portion is laterally compressed and shows blunt lateral margins with a medial sulcus that are followed by two well-developed mandibular ridges, which in turn are bordered laterally by a sulcus...
2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29166479/-challenges-and-opportunities-in-digital-health
#10
Ana O Franco, M Gabriela M Gomes
No abstract text is available yet for this article.
November 21, 2017: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/29166410/devario-in-bangladesh-species-diversity-sibling-species-and-introgression-within-danionin-cyprinids-teleostei-cyprinidae-danioninae
#11
Sven O Kullander, Md Mizanur Rahman, Michael Norén, Abdur Rob Mollah
Four species of Devario are recorded from Bangladesh: D. aequipinnatus, D. anomalus, D. coxi, new species, and D. devario. Devario aequipinnatus has a wide distribution in northern India and Bangladesh. Devario coxi, from southeastern Bangladesh near Cox's Bazar, differs from D. aequipinnatus in mtDNA (COI, p-distance 1.8%), colouration, proportional measurements, and meristics. The minor morphological differences and low frequency of overlapping meristics suggest relatively recent separation of D. coxi from other D...
2017: PloS One
https://www.readbyqxmd.com/read/29166308/an-evaluation-of-the-choice-for-contralateral-prophylactic-mastectomy-and-patient-concerns-about-recurrence-in-a-reconstructed-cohort
#12
Ian C Sando, Jessica I Billig, Shoshana W Ambani, Casey T Kraft, Kelley M Kidwell, Lin Zhong, Kevin C Chung, Adeyiza O Momoh
BACKGROUND: Rising contralateral prophylactic mastectomy rates are a subject of national concern. This study assessed (1) factors critical to patients when deciding on contralateral prophylactic mastectomy and (2) patients' quality of life related to concerns about recurrence after unilateral or bilateral breast reconstruction. METHODS: Patients with stage 0 to III breast cancer who underwent unilateral mastectomy or contralateral prophylactic mastectomy and breast reconstruction at a single institution between 2000 and 2012 were identified...
November 21, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/29166288/spirulina-platensis-affects-factors-involved-in-spermatogenesis-and-increases-ghrelin-receptors-in-testis-tissue-of-rats-fed-a-high-fat-diet
#13
O B B Esener, E Gurel-Gurevin, B Isbilen-Basok, F Yigit, T Bilal, A Altiner, N Yilmazer, E I Armutak
Ghrelin is a peptide hormone which plays important role in maintaining growth hormone release and energy homeostasis in vertebrates. Spirulina platensis (SP) has antioxidant and hypolipidemic effects due to its ingredients. In this study we aimed to investigate the effects of SP on the testicular structure and relation between ghrelin and testosterone in the testis of rats fed a high fat diet (HFD). Sixty four young adult male rats were used and divided to 8 equal groups. Experimental groups received addition of 10% cholesterol (CHL), 43% hydrogenated vegetable oil (HVO) and 3% SP alone or in combination to basal diet while the control group received only basal diet...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29166203/temporal-profile-of-care-following-mild-traumatic-brain-injury-predictors-of-hospital-admission-follow-up-referral-and-six-month-outcome
#14
John K Yue, Ethan A Winkler, Sourabh Sharma, Mary J Vassar, Jonathan J Ratcliff, Frederick K Korley, Seth A Seabury, Adam R Ferguson, Hester F Lingsma, Sacha Meeuws, Opeolu M Adeoye, Jonathan W Rick, Caitlin K Robinson, Siena M Duarte, Esther L Yuh, Pratik Mukherjee, Sureyya S Dikmen, Thomas W McAllister, Ramon Diaz-Arrastia, Alex B Valadka, Wayne A Gordon, David O Okonkwo, Geoffrey T Manley
OBJECTIVE: To investigate the clinical management and medical follow-up of patients with mild traumatic brain injury (mTBI) presenting to emergency departments (EDs). METHODS: Overall, 168 adult patients with mTBI from the prospective, multicentre Transforming Research and Clinical Knowledge in TBI (TRACK-TBI) Pilot study with Glasgow Coma Scale (GCS) 13-15, no polytrauma and alive at six months were included. Predictors for hospital admission, three-month follow-up referral and six-month functional disability (Glasgow Outcome Scale-Extended (GOSE) ≤ 6) were analysed using multivariable regression...
2017: Brain Injury: [BI]
https://www.readbyqxmd.com/read/29166161/will-invertebrates-require-increasingly-carbon-rich-food-in-a-warming-world
#15
Thomas R Anderson, Dag O Hessen, Maarten Boersma, Jotaro Urabe, Daniel J Mayor
Elevated temperature causes metabolism and respiration to increase in poikilothermic organisms. We hypothesized that invertebrate consumers will therefore require increasingly carbon-rich diets in a warming environment because the increased energetic demands are primarily met using compounds rich in carbon, that is, carbohydrates and lipids. Here, we test this hypothesis using a new stoichiometric model that has carbon (C) and nitrogen (N) as currencies. Model predictions did not support the hypothesis, indicating instead that the nutritional requirements of invertebrates, at least in terms of food quality expressed as C∶N ratio, may change little, if at all, at elevated temperature...
December 2017: American Naturalist
https://www.readbyqxmd.com/read/29166137/primary-constrained-tips-for-treating-refractory-ascites-or-variceal-bleeding-secondary-to-hepatic-cirrhosis
#16
R Rabei, S Mathesovian, J Tasse, S Madassery, B Arslan, U Turba, O Ahmed
PURPOSE: To report an initial experience using a primary constrained TIPS technique for treating cirrhotic patients with refractory ascites or variceal bleeding. METHODS: All patients undergoing primary constrained (n=9) and conventional (n=18) TIPS between July 2014 and June 2016 were retrospectively reviewed. Pre-procedure demographics, Child-Pugh (CP), MELD, and technical variables were recorded. Outcomes measured included technical and clinical success, complications, 30-day mortality, as well as necessity for TIPS revision...
November 22, 2017: British Journal of Radiology
https://www.readbyqxmd.com/read/29166062/nonadiabatic-photodissociation-dynamics-of-the-hydroxymethyl-radical-via-the-2-2-a-3s-rydberg-state-a-four-dimensional-quantum-study
#17
Changjian Xie, Christopher Malbon, David R Yarkony, Hua Guo
The quantum mechanical nonadiabatic photodissociation dynamics of the hydroxymethyl (CH2OH) radical in its lowest absorption band is investigated for the first time on a set of coupled diabatic potential energy surfaces determined by accurately fitting a large set of ab initio data. In this two-state approximation, only the ground and first excited states of CH2OH, which are coupled by conical intersections, are included. The reduced-dimensional dynamical model includes the CO stretch, the COH bend, the HCOH torsion, and the O-H dissociation coordinate...
June 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29166060/origin-of-oer-catalytic-activity-difference-of-oxygen-deficient-perovskites-a2mn2o5-a-ca-sr-a-theoretical-study
#18
Xiaolong Yao, Jieyu Liu, Weihua Wang, Feng Lu, Weichao Wang
Mn-based oxygen-deficient perovskite catalysts A2Mn2O5 (A = Ca, Sr) have been experimentally proved high oxygen evolution reaction (OER) activities for replacing Pt in oxygen electrocatalysis. Nevertheless, the correlation between the fundamental electronic structure at room temperature and the corresponding electrocatalysis is not fully accessible. In this paper, we combine the ground state density functional theory (DFT) method and dynamic mean-field theory (DFT+DMFT) at room temperature to investigate the origin of the OER difference for electrocatalysts A2Mn2O5 (A = Ca, Sr)...
June 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29166047/ortho-para-interconversion-in-cation-water-complexes-the-case-of-v-h2o-and-nb-h2o-clusters
#19
T B Ward, E Miliordos, P D Carnegie, S S Xantheas, M A Duncan
Vanadium and niobium cation-water complexes, V(+)(H2O) and Nb(+)(H2O), are produced by laser vaporization in a pulsed supersonic expansion, mass selected in a time-of-flight spectrometer, and studied with infrared photodissociation spectroscopy using rare gas atom (Ar, Ne) complex predissociation. The vibrational bands measured in the O-H stretching region contain K-type rotational sub-band structure, which provides insight into the structures of these complexes. However, rotational sub-bands do not exhibit the simple patterns seen previously for other metal ion-water complexes...
June 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29166042/laplace-transformed-multi-reference-second-order-perturbation-theories-in-the-atomic-and-active-molecular-orbital-basis
#20
Benjamin Helmich-Paris, Stefan Knecht
In the present article, we show how to formulate the partially contracted n-electron valence second-order perturbation theory (NEVPT2) energies in the atomic and active molecular orbital basis by employing the Laplace transformation of orbital-energy denominators (OEDs). As atomic-orbital (AO) basis functions are inherently localized and the number of active orbitals is comparatively small, our formulation is particularly suited for a linearly scaling NEVPT2 implementation. In our formulation, there are two kinds of NEVPT2 energy contributions, which differ in the number of active orbitals in the two-electron integrals involved...
June 14, 2017: Journal of Chemical Physics
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