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Bioremediation

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https://www.readbyqxmd.com/read/29793204/influence-of-isolated-bacterial-strains-on-the-in-situ-biodegradation-of-endosulfan-and-the-reduction-of-endosulfan-contaminated-soil-toxicity
#1
Lingfen Kong, Yu Zhang, Lusheng Zhu, Jinhua Wang, Jun Wang, Zhongkun Du, Cheng Zhang
The recently discovered endosulfan-degrading bacterial strains Pusillimonas sp. JW2 and Bordetella petrii NS were isolated from endosulfan-polluted water and soil environments. The optimal conditions for the growth and biodegradation activity of the strains JW2 and NS were studied in detail. In addition, the ability of the strains JW2 and NS to biodegrade endosulfan in soils during in situ bioremediation experiments was investigated. At a concentration of 2 mg of endosulfan per kilogram of soil, both JW2 and NS had positive effects on the degradation of endosulfan; JW2 degraded 100% and 91...
May 21, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29792416/phosphotriesterase-magnetic-nanoparticles-bioconjugates-with-improved-enzyme-activity-in-a-biocatalytic-membrane-reactor
#2
Abaynesh Yihdego Gebreyohannes, Rosalinda Mazzei, Mohamed Yahia Marei Abdelrahim, Giuseppe Vitola, Elena Porzio, Giuseppe Manco, Mihail Barboiu, Lidietta Giorno
The need to find alternative bioremediation solutions for organophosphate degradation pushed the research to develop technologies based on organophosphate degrading enzymes, such as phosphotriesterase. The use of free phosphotriesterase poses limits in terms of enzyme reuse, stability and process development. The heterogenization of enzyme on a support and their use in bioreactors implemented by membrane seems a suitable strategy, thanks to the ability of membranes to compartmentalize, to govern mass transfer and provide microenvironment with tuned physico-chemical and structural properties...
May 24, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29779193/functional-role-of-tyr12-in-the-catalytic-activity-of-novel-zeta-like-glutathione-s-transferase-from-acidovorax-sp-kks102
#3
Dayyabu Shehu, Zazali Alias
Glutathione S-transferases (GSTs) are a family of enzymes that function in the detoxification of variety of electrophilic substrates. In the present work, we report a novel zeta-like GST (designated as KKSG9) from the biphenyl/polychlorobiphenyl degrading organism Acidovorax sp. KKS102. KKSG9 possessed low sequence similarity but similar biochemical properties to zeta class GSTs. Functional analysis showed that the enzyme exhibits wider substrate specificity compared to most zeta class GSTs by reacting with 1-chloro-2,4-dinitrobenzene (CDNB), p-nitrobenzyl chloride (NBC), ethacrynic acid (EA), hydrogen peroxide, and cumene hydroperoxide...
May 19, 2018: Protein Journal
https://www.readbyqxmd.com/read/29777442/study-on-immobilization-of-marine-oil-degrading-bacteria-by-carrier-of-algae-materials
#4
Yiran Zhang, Wei Gao, Faxiang Lin, Bin Han, Changfei He, Qian Li, Xiangxing Gao, Zhisong Cui, Chengjun Sun, Li Zheng
This study investigated the immobilizations with of bacteria two kinds of algal materials, Enteromorpha residue and kelp residue. The lipophilicity of them were compared by diesel absorption rates. The immobilization efficiency of Bacillus sp. E3 was measured to evaluate whether these carriers would satisfy the requirement for biodegradation of oil spills. The bacteria were immobilized through adsorption with the sterilized and non-sterilized carriers to compare the differences between the two treatments. Oil degradation rates were determined using gravimetric and GC-MS methods...
May 18, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29776370/characterization-of-a-promiscuous-cadmium-and-arsenic-resistance-mechanism-in-thermus-thermophilus-hb27-and-potential-application-of-a-novel-bioreporter-system
#5
Immacolata Antonucci, Giovanni Gallo, Danila Limauro, Patrizia Contursi, Ana Luisa Ribeiro, Alba Blesa, José Berenguer, Simonetta Bartolucci, Gabriella Fiorentino
BACKGROUND: The characterization of the molecular determinants of metal resistance has potential biotechnological application in biosensing and bioremediation. In this context, the bacterium Thermus thermophilus HB27 is a metal tolerant thermophile containing a set of genes involved in arsenic resistance which, differently from other microbes, are not organized into a single operon. They encode the proteins: arsenate reductase, TtArsC, arsenic efflux membrane transporter, TtArsX, and transcriptional repressor, TtSmtB...
May 18, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29775827/responses-of-atrazine-degradation-and-native-bacterial-community-in-soil-to-arthrobacter-sp-strain-hb-5
#6
Jianpeng Gao, Peipei Song, Guanying Wang, Jinhua Wang, Lusheng Zhu, Jun Wang
The bioremediation of soil contaminated with organic pesticides is a safe and effective approach to remove pollutants from the soil. However, whether the invasion of foreign aid organisms affects the local organisms has received increasing attention in recent years. Therefore, the purpose of this study was to examine the degradation ability of atrazine by the strain HB-5 and evaluate its effects on natural bacterial communities in a miniature pot experiment. Results showed that HB-5 accelerated the degradation of atrazine and the degradation half-life of atrazine was 3...
May 15, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29775704/alkaline-active-cyanide-dihydratase-of-flavobacterium-indicum-mtcc-6936-growth-optimization-purification-characterization-and-in-silico-analysis
#7
Virender Kumar, Vijay Kumar, Tek Chand Bhalla
The present work explores a rare cyanide dihydratase of Flavobacterium indicum MTCC 6936 for its potential of cyanide degradation. The enzyme is purified to 12 fold with a yield of 76%. SDS and native-PAGE analysis revealed that enzyme was monomer of 40 kDa size. The enzyme works well in mesophilic range at wide array of pH. The thermostability profile of cyanide dihydratase revealed that the enzyme is quite stable at 30 °C and 35 °C with half-life of 6 h 30 min and 5 h respectively. Km and Vmax for cyanide dihydratase of F...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29774512/correction-to-ecotoxicological-assessment-of-oil-based-paint-using-three-dimensional-multi-species-bio-testing-model-pre-and-post-bioremediation-analysis
#8
Anwar Hussain Phulpoto, Muneer Ahmed Qazi, Ihsan Ul Haq, Abdul Rahman Phul, Safia Ahmed, Nisar Ahmed Kanhar
The original publication of this paper contains a mistake.
May 17, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29773745/prospects-for-harnessing-biocide-resistance-for-bioremediation-and-detoxification
#9
REVIEW
Siavash Atashgahi, Irene Sánchez-Andrea, Hermann J Heipieper, Jan R van der Meer, Alfons J M Stams, Hauke Smidt
Prokaryotes in natural environments respond rapidly to high concentrations of chemicals and physical stresses. Exposure to anthropogenic toxic substances-such as oil, chlorinated solvents, or antibiotics-favors the evolution of resistant phenotypes, some of which can use contaminants as an exclusive carbon source or as electron donors and acceptors. Microorganisms similarly adapt to extreme pH, metal, or osmotic stress. The metabolic plasticity of prokaryotes can thus be harnessed for bioremediation and can be exploited in a variety of ways, ranging from stimulated natural attenuation to bioaugmentation and from wastewater treatment to habitat restoration...
May 18, 2018: Science
https://www.readbyqxmd.com/read/29772529/microbial-bio-fuels-a-solution-to-carbon-emissions-and-energy-crisis
#10
Arun Kumar, Sumit Kaushal, Shubhini A Saraf, Jay Shankar Singh
Increasing energy demand, limited fossil fuel resources and climate change have prompted development of alternative sustainable and economical fuel resources such as crop-based bio-ethanol and bio-diesel. However, there is concern over use of arable land that is used for food agriculture for creation of biofuel. Thus, there is a renewed interest in the use of microbes particularly microalgae for bio-fuel production. Microbes such as micro-algae and cyanobacteria that are used for biofuel production also produce other bioactive compounds under stressed conditions...
June 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/29772463/bioremediation-of-organophosphorus-pesticide-phorate-in-soil-by-microbial-consortia
#11
Monu Jariyal, Vikas Jindal, Kousik Mandal, Virash Kamal Gupta, Balwinder Singh
Microbial consortia isolated from aged phorate contaminated soil were used to degrade phorate. The consortia of three microorganisms (Brevibacterium frigoritolerans, Bacillus aerophilus and Pseudomonas fulva) could degrade phorate, and the highest phorate removal (between 97.65 and 98.31%) was found in soils inoculated with mixed cultures of all the three bacterial species. However, the mixed activity of any of two of these bacteria was lower than mixed consortia of all the three bacterial species. The highest degradation by individual mixed consortia of (B...
May 14, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29772382/enhanced-biodegradation-of-hydrocarbons-in-petroleum-tank-bottom-oil-sludge-and-characterization-of-biocatalysts-and-biosurfactants
#12
S Hepziba Suganthi, Shabnam Murshid, Sriswarna Sriram, K Ramani
Petroleum hydrocarbon removal from tank bottom oil sludge is a major issue due to its properties. Conventional physicochemical treatment techniques are less effective. Though the bioremediation is considered for the hydrocarbon removal from tank bottom oil sludge, the efficiency is low and time taking due to the low yield of biocatalysts and biosurfactants. The focal theme of the present investigation is to modify the process by introducing the intermittent inoculation for the enhanced biodegradation of hydrocarbons in the tank bottom oil sludge by maintaining a constant level of biocatalysts such as oxidoreductase, catalase, and lipase as well as biosurfactants...
May 14, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29771316/toluene-degradation-by-cupriavidus-metallidurans-ch34-in-nitrate-reducing-conditions-and-in-bioelectrochemical-systems
#13
Anna Espinoza Tofalos, Matteo Daghio, Myriam González, Maddalena Papacchini, Andrea Franzetti, Michael Seeger
Bioelectrochemical remediation of hydrocarbons is a technology that exploits the ability of specific microorganisms to use as electron acceptor an electrode, thus potentially lowering the operational costs related to classical bioremediation. Several well-characterized hydrocarbonoclastic strains might be electroactive, thus their biodegradation performances in Bioelectrochemical Systems should be studied. Cupriavidus metallidurans CH34 is a model metal-resistant strain whose capacity to degrade benzene aerobically has recently been described...
May 16, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29768233/biogenic-gold-nanoparticles-for-reduction-of-4-nitrophenol-to-4-aminophenol-an-eco-friendly-bioremediation
#14
Asmathunisha Nabikhan, Suji Rathinam, Kathiresan Kandasamy
The present study investigated the synthesis of gold nanoparticles (AuNPs) using mangrove plant extract from Avicennia marina as bioreductant for eco-friendly bioremediation of 4-nitrophenol (4-NP) . The AuNPs synthesised were confirmed by UV spectrum, transmission electron microscopy (TEM), X-ray diffraction, Fourier transmission infrared spectroscopy (FTIR), dynamic light scattering (DLS), and zeta potential. The AuNPs were found to be spherical in shape with size ranging from 4 to 13 nm, as evident by TEM and DLS...
June 2018: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29765091/metagenomic-profiling-for-assessing-microbial-diversity-and-microbial-adaptation-to-degradation-of-hydrocarbons-in-two-south-african-petroleum-contaminated-water-aquifers
#15
Leonard Kachienga, Keshri Jitendra, Maggy Momba
Biodegradation of hydrocarbons by indigenous populations of microorganisms found in petroleum-contaminated water sources represents one of the primary mechanisms by which petroleum and other hydrocarbon pollutants are eliminated from the aquatic environment. The identification of these microorganisms, which have capabilities to convert the majority of toxic hydrocarbons into compounds that are less harmful for end-users, is therefore crucial for bioremediation purposes. The aim of this study was to profile the microbial diversity of two South African petroleum-contaminated water aquifer sites and to determine the microbial adaptation to hydrocarbon degradation using a metagenomics approach...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29764204/biotechnological-potential-of-microbial-consortia-and-future-perspectives
#16
Shashi Kant Bhatia, Ravi Kant Bhatia, Yong-Keun Choi, Eunsung Kan, Yun-Gon Kim, Yung-Hun Yang
Design of a microbial consortium is a newly emerging field that enables researchers to extend the frontiers of biotechnology from a pure culture to mixed cultures. A microbial consortium enables microbes to use a broad range of carbon sources. It provides microbes with robustness in response to environmental stress factors. Microbes in a consortium can perform complex functions that are impossible for a single organism. With advancement of technology, it is now possible to understand microbial interaction mechanism and construct consortia...
May 15, 2018: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/29763873/estimates-of-abundance-and-diversity-of-shewanella-genus-in-natural-and-engineered-aqueous-environments-with-newly-designed-primers
#17
Bing-Bing Li, Yuan-Yuan Cheng, Yang-Yang Fan, Dong-Feng Liu, Cai-Yun Fang, Chao Wu, Wen-Wei Li, Zong-Chuang Yang, Han-Qing Yu
Shewanella species have a diverse respiratory ability and wide distribution in environments and play an important role in bioremediation and the biogeochemical cycles of elements. Primers with more accuracy and broader coverage are required with consideration of the increasing number of Shewanella species and evaluation of their roles in various environments. In this work, a new primer set of 640F/815R was developed to quantify the abundance of Shewanella species in natural and engineered environments. In silico tools for primer evaluation, quantitative polymerase chain reaction (qPCR) and clone library results showed that 640F/815R had a higher specificity and coverage than the previous primers in quantitative analysis of Shewanella...
May 12, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29763639/complete-genome-sequence-of-gordonia-sp-yc-jh1-a-bacterium-efficiently-degrading-a-wide-range-of-phthalic-acid-esters
#18
Shuanghu Fan, Junhuan Wang, Kang Li, Ting Yang, Yang Jia, Baisuo Zhao, Yanchun Yan
Phthalic acid esters (PAEs) are a family of recalcitrant pollutants mainly used as plasticizer. The strain Gordonia sp.YC-JH1, isolated from petroleum-contaminated soil, is capable of efficiently degrading a wide range of PAEs. In order to pertinently investigate the genetic mechanisms of PAEs catabolism by strain YC-JH1, its complete genome sequencing has been performed by SMRT sequencing technology. The genome comprises a circular chromosome and a plasmid with a size of 4,101,557 bp and 91,767 bp respectively...
May 12, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29755441/a-genomic-outlook-on-bioremediation-the-case-of-arsenic-removal
#19
REVIEW
Frédéric Plewniak, Simona Crognale, Simona Rossetti, Philippe N Bertin
Microorganisms play a major role in biogeochemical cycles. As such they are attractive candidates for developing new or improving existing biotechnological applications, in order to deal with the accumulation and pollution of organic and inorganic compounds. Their ability to participate in bioremediation processes mainly depends on their capacity to metabolize toxic elements and catalyze reactions resulting in, for example, precipitation, biotransformation, dissolution, or sequestration. The contribution of genomics may be of prime importance to a thorough understanding of these metabolisms and the interactions of microorganisms with pollutants at the level of both single species and microbial communities...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29754988/bioremediation-of-petroleum-contaminated-soil-using-aged-refuse-from-landfills
#20
Qingmei Liu, Qibin Li, Ning Wang, Dan Liu, Li Zan, Le Chang, Xuemei Gou, Peijin Wang
This study explored the effects and mechanisms of petroleum-contaminated soil bioremediation using aged refuse (AR) from landfills. Three treatments of petroleum-contaminated soil (47.28 mg·g-1 ) amended with AR, sterilized aged refuse (SAR) and petroleum-contaminated soil only (as a control) were tested. During 98 days of incubation, changes in soil physicochemical properties, residual total petroleum hydrocarbon (TPH), biodegradation kinetics, enzyme activities and the microbial community were investigated...
May 10, 2018: Waste Management
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