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https://www.readbyqxmd.com/read/28330093/exploration-of-nifh-gene-through-soil-metagenomes-of-the-western-indian-himalayas
#1
Ravindra Soni, Deep Chandra Suyal, Santosh Sai, Reeta Goel
This group has previously highlighted the prevalence of Csp genes from cold Himalayan environments. However, this study has explored the uncultured diazotrophs from metagenomes of western Indian Himalayas. The metagenomic nifH gene clone library was constructed from the Temperate, Subtropical and Tarai soils of Western Himalaya, India followed by polymerase chain reaction (PCR) amplification. After preliminary screening, selected clones were sequenced. In silico analysis of the clones was done, which documented 83...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28295106/structure-electrocatalysis-and-dynamics-of-immobilized-cytochrome-pcch-and-its-microperoxidase
#2
Célia M Silveira, María A Castro, Joana M Dantas, Carlos Salgueiro, Daniel H Murgida, Smilja Todorovic
Geobacter sulfurreducens cells have the ability to exchange electrons with conductive materials, and the periplasmic cytochrome PccH plays an essential role in the direct electrode-to-cell electron transfer in this bacterium. It has atypically low redox potential and unique structural features that differ from those observed in other c-type cytochromes. We report surface enhanced resonance Raman spectroscopic and electrochemical characterization of the immobilized PccH, together with molecular dynamics simulations that allow for the rationalization of experimental observations...
March 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28287150/cooperative-growth-of-geobacter-sulfurreducens-and-clostridium-pasteurianum-with-subsequent-metabolic-shift-in-glycerol-fermentation
#3
Roman Moscoviz, Florence de Fouchécour, Gaëlle Santa-Catalina, Nicolas Bernet, Eric Trably
Interspecies electron transfer is a common way to couple metabolic energy balances between different species in mixed culture consortia. Direct interspecies electron transfer (DIET) mechanism has been recently characterised with Geobacter species which couple the electron balance with other species through physical contacts. Using this mechanism could be an efficient and cost-effective way to directly control redox balances in co-culture fermentation. The present study deals with a co-culture of Geobacter sulfurreducens and Clostridium pasteurianum during glycerol fermentation...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28279910/response-of-microbial-community-structure-to-pre-acclimation-strategies-in-microbial-fuel-cells-for-domestic-wastewater-treatment
#4
Younghyun Park, Hyunwoo Cho, Jaechul Yu, Booki Min, Hong Suck Kim, Byung Goon Kim, Taeho Lee
Microbial community structures and performance of air-cathode microbial fuel cells (MFCs) inoculated with activated sludge from domestic wastewater were investigated to evaluate the effects of three substrate pre-acclimation strategies: 1, serial pre-acclimation with acetate and glucose before supplying domestic wastewater; 2, one step pre-acclimation with acetate before supplying domestic wastewater; and 3, direct supply of domestic wastewater without any pre-acclimation. Strategy 1 showed much higher current generation (1...
February 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28273578/polarity-inversion-of-bioanode-for-biocathodic-reduction-of-aromatic-pollutants
#5
Hui Yun, Bin Liang, De-Yong Kong, Hao-Yi Cheng, Zhi-Ling Li, Ya-Bing Gu, Hua-Qun Yin, Ai-Jie Wang
The enrichment of specific pollutant-reducing consortium is usually required prior to the startup of biocathode bioelectrochemical system (BES) and the whole process is time consuming. To rapidly establish a non-specific functional biocathode, direct polar inversion from bioanode to biocathode is proposed in this study. Based on the diverse reductases and electron transfer related proteins of anode-respiring bacteria (ARB), the acclimated electrochemically active biofilm (EAB) may catalyze reduction of different aromatic pollutants...
February 27, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28258137/metatranscriptomic-evidence-for-direct-interspecies-electron-transfer-between-geobacter-and-methanothrix-species-in-methanogenic-rice-paddy-soils
#6
Dawn E Holmes, Pravin M Shrestha, David J F Walker, Yan Dang, Kelly P Nevin, Trevor L Woodard, Derek R Lovley
The possibility that Methanothrix (formerly Methanosaeta) and Geobacter species cooperate via direct interspecies electron transfer (DIET) in terrestrial methanogenic environments was investigated in rice paddy soils. Genes with high sequence similarity to the gene for the PilA pilin monomer of the electrically conductive pili (e-pili) of Geobacter sulfurreducens accounted for over half of the PilA gene sequences in metagenomic libraries and 42% of the mRNA transcripts in RNAseq libraries. This abundance of e-pilin genes and transcripts is significant because e-pili can serve as conduits for DIET...
March 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28254576/effect-of-short-term-alkaline-intervention-on-the-performance-of-buffer-free-single-chamber-microbial-fuel-cell
#7
Na Yang, Yueping Ren, Xiufen Li, Xinhua Wang
Anolyte acidification is a drawback restricting the electricity generation performance of the buffer-free microbial fuel cells (MFC). In this paper, a small amount of alkali-treated anion exchange resin (AER) was placed in front of the anode in the KCl mediated single-chamber MFC to slowly release hydroxyl ions (OH(-)) and neutralize the H(+) ions that are generated by the anodic reaction in two running cycles. This short-term alkaline intervention to the KCl anolyte has promoted the proliferation of electroactive Geobacter sp...
June 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28254049/performance-of-low-temperature-microbial-fuel-cells-mfcs-catalyzed-by-mixed-bacterial-consortia
#8
Olga Tkach, Thangavel Sangeetha, Spiridonova Maria, Aijie Wang
Microbial Fuel Cells (MFCs) are a promising technology for treating wastewater in a sustainable manner. In potential applications, low temperatures substantially reduce MFC performance. To better understand the effect of temperature and particularly how bioanodes respond to changes in temperature, we investigated the current generation of mixed-culture and pure-culture MFCs at two low temperatures, 10°C and 5°C. The results implied that the mixed-culture MFC sustainably performed better than the pure-culture (Shewanella) MFC at 10°C, but the electrogenic activity of anodic bacteria was substantially reduced at the lower temperature of 5°C...
February 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28246922/investigating-the-microbial-degradation-potential-in-oil-sands-fluid-fine-tailings-using-gamma-irradiation-a-metagenomic-perspective
#9
Danielle VanMensel, Subba Rao Chaganti, Ryan Boudens, Thomas Reid, Jan Ciborowski, Christopher Weisener
Open-pit mining of the Athabasca oil sands has generated large volumes of waste termed fluid fine tailings (FFT), stored in tailings ponds. Accumulation of toxic organic substances in the tailings ponds is one of the biggest concerns. Gamma irradiation (GI) treatment could accelerate the biodegradation of toxic organic substances. Hence, this research investigates the response of the microbial consortia in GI-treated FFT materials with an emphasis on changes in diversity and organism-related stimuli. FFT materials from aged and fresh ponds were used in the study under aerobic and anaerobic conditions...
March 1, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28242707/oligomeric-lipoprotein-pelc-guides-pel-polysaccharide-export-across-the-outer-membrane-of-pseudomonas-aeruginosa
#10
Lindsey S Marmont, Jacquelyn D Rich, John C Whitney, Gregory B Whitfield, Henrik Almblad, Howard Robinson, Matthew R Parsek, Joe J Harrison, P Lynne Howell
Secreted polysaccharides are important functional and structural components of bacterial biofilms. The opportunistic pathogen Pseudomonas aeruginosa produces the cationic exopolysaccharide Pel, which protects bacteria from aminoglycoside antibiotics and contributes to biofilm architecture through ionic interactions with extracellular DNA. A bioinformatics analysis of genome databases suggests that gene clusters for Pel biosynthesis are present in >125 bacterial species, yet little is known about how this biofilm exopolysaccharide is synthesized and exported from the cell...
February 27, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28220237/microbial-fuel-cell-coupled-to-biohydrogen-reactor-a-feasible-technology-to-increase-energy-yield-from-cheese-whey
#11
J Wenzel, L Fuentes, A Cabezas, C Etchebehere
An important pollutant produced during the cheese making process is cheese whey which is a liquid by-product with high content of organic matter, composed mainly by lactose and proteins. Hydrogen can be produced from cheese whey by dark fermentation but, organic matter is not completely removed producing an effluent rich in volatile fatty acids. Here we demonstrate that this effluent can be further used to produce energy in microbial fuel cells. Moreover, current production was not feasible when using raw cheese whey directly to feed the microbial fuel cell...
February 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28214441/enhanced-treatment-of-fischer-tropsch-f-t-wastewater-using-the-up-flow-anaerobic-sludge-blanket-coupled-with-bioelectrochemical-system-effect-of-electric-field
#12
Dexin Wang, Hongjun Han, Yuxing Han, Kun Li, Hao Zhu
The coupling of bioelectrochemical system (BES) with an up-flow anaerobic sludge blanket (UASB) was established for enhanced Fischer-Tropsch (F-T) wastewater treatment while the UASB (control group) was operated in parallel. The presence of electric field could offer system a more reductive micro-environment that lower the ORP values and maintain the appropriate pH range, resulting in the higher chemical oxygen demand (COD) removal efficiency and methane production for BES-UASB (86.8% and 2.31±0.1L/(L·d)) while those values in control group were 72...
February 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28213734/dehalococcoides-and-general-bacterial-ecology-of-differentially-trichloroethene-dechlorinating-flow-through-columns
#13
Babur S Mirza, Darwin L Sorensen, Daniel J McGlinn, R Ryan Dupont, Joan E McLean
The diversity of Dehalococcoides mccartyi (Dhc) and/or other organohalide respiring or associated microorganisms in parallel, partial, or complete trichloroethene (TCE) dehalogenating systems has not been well described. The composition of Dhc populations and the associated bacterial community that developed over 7.5 years in the top layer (0-10 cm) of eight TCE-fed columns were examined using pyrosequencing. Columns biostimulated with one of three carbon sources, along with non-stimulated controls, developed into complete (ethene production, whey amended), partial (cis-dichloroethene (DCE) and VC, an emulsified oil with nonionic surfactant), limited (<5 % cis-DCE and 95 % TCE, an emulsified oil), and non- (controls) TCE dehalogenating systems...
February 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28188105/microbial-community-composition-and-function-in-a-pilot-scale-anaerobic-anoxic-aerobic-combined-process-for-the-treatment-of-traditional-chinese-medicine-wastewater
#14
Longyi Lv, Weiguang Li, Chuandong Wu, Liqiang Meng, Wen Qin
Biodegradation of traditional Chinese medicine (TCM) wastewater was investigated in a pilot-scale anaerobic-anoxic-aerobic combined process, which was composed of an expanded granular sludge blanket (EGSB) reactor, a hydrolysis acidification (HA) reactor and a biological contact oxidation (BCO) reactor. In stable stage, the average values of COD and color in the combined process effluent were 45.7mgL(-1) and 13 times, respectively. Excellent linear relations (R(2)>0.915) were achieved between color and UV254 at three color levels...
January 30, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28183017/effect-of-the-redox-dynamics-on-microbial-mediated-as-transformation-coupled-with-fe-and-s-in-flow-through-sediment-columns
#15
Hee Sun Moon, Bo-A Kim, Sung Pil Hyun, Yoon-Ho Lee, Doyun Shin
Arsenic (As) biogeochemistry coupled with iron (Fe) and sulfur (S) was studied using columns packed with As(V)-contaminated sediments under two phases: a reduction phase followed by an oxidation phase. During the reduction phase, four identical columns inoculated with G. sulfurreducens were stimulated with 3mM acetate for 60days. The As(III) in the effluent rapidly increased then gradually decreased. The Fe(II) and sulfate concentration indicated ferrous sulfide precipitation inside the column after day 14 and X-ray absorption near edge structure spectra showed that As(III) was enriched at the column outlet...
May 5, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28139786/molecular-interactions-between-geobacter-sulfurreducens-triheme-cytochromes-and-the-electron-acceptor-fe-iii-citrate-studied-by-nmr
#16
Marisa R Ferreira, Joana M Dantas, Carlos A Salgueiro
Proteomic and genetic studies have identified a family of five triheme cytochromes (PpcA-E) that are essential in the iron respiratory pathways of Geobacter sulfurreducens. These include the reduction of Fe(iii) soluble chelated forms or Fe(iii) oxides, which can be used as terminal acceptors by G. sulfurreducens. The relevance of these cytochromes in the respiratory pathways of soluble or insoluble forms of iron is quite distinct. In fact, while PpcD had a higher abundance in the Fe(iii) oxides supplanted G...
January 31, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28138101/significance-of-a-post-translational-modification-of-the-pila-protein-of-geobacter-sulfurreducens-for-surface-attachment-biofilm-formation-and-growth-on-insoluble-extracellular-electron-acceptors
#17
Lubna V Richter, Ashley E Franks, Robert M Weis, Steven J Sandler
Geobacter sulfurreducens, an anaerobic metal-reducing bacterium, possesses type IV pili. These pili are intrinsic structural elements in biofilm formation and together with a number of c-type cytochromes are thought to serve as conductive nanowires enabling long-range electron transfer (ET) to metal oxides and graphite anodes. Here, we report that a post-translational modification of a non-conserved amino acid residue within the PilA protein, the structural subunit of the type IV pili, is crucial for growth on insoluble extracellular electron acceptors...
January 30, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28096491/expressing-the-geobacter-metallireducens-pila-in-geobacter-sulfurreducens-yields-pili-with-exceptional-conductivity
#18
Yang Tan, Ramesh Y Adhikari, Nikhil S Malvankar, Joy E Ward, Trevor L Woodard, Kelly P Nevin, Derek R Lovley
The electrically conductive pili (e-pili) of Geobacter sulfurreducens serve as a model for a novel strategy for long-range extracellular electron transfer. e-pili are also a new class of bioelectronic materials. However, the only other Geobacter pili previously studied, which were from G. uraniireducens, were poorly conductive. In order to obtain more information on the range of pili conductivities in Geobacter species, the pili of G. metallireducens were investigated. Heterologously expressing the PilA gene of G...
January 17, 2017: MBio
https://www.readbyqxmd.com/read/28093471/interactions-studies-between-periplasmic-cytochromes-provide-insights-on-extracellular-electron-transfer-pathways-of-geobacter-sulfurreducens
#19
Ana P Fernandes, Tiago C Nunes, Catarina M Paquete, Carlos A Salgueiro
Geobacter bacteria usually prevail among other microorganisms in soils and sediments where Fe(III) reduction has a central role. This reduction is achieved by extracellular electron transfer, where the electrons are exported from the interior of the cell to the surrounding environment. Periplasmic cytochromes play an important role in establishing an interface between inner and outer membrane electron transfer components. In addition, periplasmic cytochromes, in particular nanowire cytochromes that contain at least 12 haem groups, have been proposed to play a role in electron storage in conditions of environmental lack of electron acceptors...
January 16, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28087529/resilience-dynamics-and-interactions-within-a-model-multispecies-exoelectrogenic-biofilm-community
#20
Anna Prokhorova, Katrin Sturm-Richter, Andreas Doetsch, Johannes Gescher
Anode-associated multispecies exoelectrogenic biofilms are essential for the function of bioelectrochemical systems (BESs). The individual activities of anode-associated organisms and physiological responses resulting from coculturing are often hard to assess due to the high microbial diversity in these systems. Therefore, we developed a model multispecies biofilm comprising three exoelectrogenic proteobacteria, Shewanella oneidensis, Geobacter sulfurreducens, and Geobacter metallireducens, with the aim to study in detail the biofilm formation dynamics, the interactions between the organisms, and the overall activity of an exoelectrogenic biofilm as a consequence of the applied anode potential...
March 15, 2017: Applied and Environmental Microbiology
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