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https://www.readbyqxmd.com/read/28513579/electricity-from-methane-by-reversing-methanogenesis
#1
Michael J McAnulty, Venkata G Poosarla, Kyoung-Yeol Kim, Ricardo Jasso-Chávez, Bruce E Logan, Thomas K Wood
Given our vast methane reserves and the difficulty in transporting methane without substantial leaks, the conversion of methane directly into electricity would be beneficial. Microbial fuel cells harness electrical power from a wide variety of substrates through biological means; however, the greenhouse gas methane has not been used with much success previously as a substrate in microbial fuel cells to generate electrical current. Here we construct a synthetic consortium consisting of: (i) an engineered archaeal strain to produce methyl-coenzyme M reductase from unculturable anaerobic methanotrophs for capturing methane and secreting acetate; (ii) micro-organisms from methane-acclimated sludge (including Paracoccus denitrificans) to facilitate electron transfer by providing electron shuttles (confirmed by replacing the sludge with humic acids), and (iii) Geobacter sulfurreducens to produce electrons from acetate, to create a microbial fuel cell that converts methane directly into significant electrical current...
May 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28497287/role-of-nitrite-in-the-competition-between-denitrification-and-dnra-in-a-chemostat-enrichment-culture
#2
Eveline M van den Berg, Julius L Rombouts, J Gijs Kuenen, Robbert Kleerebezem, Mark C M van Loosdrecht
Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine the role of nitrite versus nitrate as terminal electron acceptor on the competition between DNRA and denitrification. Initially, a mixed culture chemostat was operated under nitrate limitation and performed DNRA. Stepwise, the influent nitrate was replaced with nitrite until nitrite was the sole electron acceptor and N-source present...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28494298/remediation-of-pah-polluted-soils-using-a-soil-microbial-fuel-cell-influence-of-electrode-interval-and-role-of-microbial-community
#3
Bao Yu, Jing Tian, Liu Feng
The soil microbial fuel cells (SMFCs) were constructed to remediate soils contaminated by polycyclic aromatic hydrocarbons (PAHs). With a maximum power density of 12.1mWm(-2) and an internal resistance of 470Ω, a closed SMFC showed electricity generation comparable to that by an open SMFC after 175days of operation and meanwhile increased the removal rates of anthracene, phenanthrene, and pyrene to 54.2±2.7%, 42.6±1.9% and 27.0±2.1% from 20.8±1.1%, 17.3±1.2% and 11.7±0.9%, respectively, by the open SMFC...
April 28, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28487680/diversity-of-metabolically-active-bacteria-in-water-flooded-high-temperature-heavy-oil-reservoir
#4
Tamara N Nazina, Natalya M Shestakova, Ekaterina M Semenova, Alena V Korshunova, Nadezda K Kostrukova, Tatiana P Tourova, Liu Min, Qingxian Feng, Andrey B Poltaraus
The goal of this work was to study the overall genomic diversity of microorganisms of the Dagang high-temperature oilfield (PRC) and to characterize the metabolically active fraction of these populations. At this water-flooded oilfield, the microbial community of formation water from the near-bottom zone of an injection well where the most active microbial processes of oil degradation occur was investigated using molecular, cultural, radiotracer, and physicochemical techniques. The samples of microbial DNA and RNA from back-flushed water were used to obtain the clone libraries for the 16S rRNA gene and cDNA of 16S rRNA, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28484799/distribution-of-anaerobic-hydrocarbon-degrading-bacteria-in-soils-from-king-george-island-maritime-antarctica
#5
Dayanna Souza Sampaio, Juliana Rodrigues Barboza Almeida, Hugo E de Jesus, Alexandre S Rosado, Lucy Seldin, Diogo Jurelevicius
Anaerobic diesel fuel Arctic (DFA) degradation has already been demonstrated in Antarctic soils. However, studies comparing the distribution of anaerobic bacterial groups and of anaerobic hydrocarbon-degrading bacteria in Antarctic soils containing different concentrations of DFA are scarce. In this study, functional genes were used to study the diversity and distribution of anaerobic hydrocarbon-degrading bacteria (bamA, assA, and bssA) and of sulfate-reducing bacteria (SRB-apsR) in highly, intermediate, and non-DFA-contaminated soils collected during the summers of 2009, 2010, and 2011 from King George Island, Antarctica...
May 8, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28484115/microbially-reduced-graphene-oxide-shows-efficient-electricity-ecovery-from-artificial-dialysis-wastewater
#6
Yuko Goto, Naoko Yoshida
Anodes are crucial in determining the electricity recovery of microbial fuel cells (MFCs). In this study, graphene oxide (GO) was used as an anodic material for electricity recovery from artificial dialysis wastewater (ADWW). Anaerobic incubation of ADWW with GO for 21 days produced a hydrogel complex containing embedded microbial cells and microbially reduced GO (rGO). The rGO complex recovered 540 to 810 μA/cm(3) of catalytic current from ADWW after 10 days of electrochemical cultivation at 200 mV (vs. Ag/AgCl), which was approximately thirty times higher than that recovered from graphite felt (GF), a representative anode in MFCs...
May 2, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28474682/the-molecular-mechanism-of-the-type-iva-pilus-motors
#7
Matthew McCallum, Stephanie Tammam, Ahmad Khan, Lori L Burrows, P Lynne Howell
Type IVa pili are protein filaments essential for virulence in many bacterial pathogens; they extend and retract from the surface of bacterial cells to pull the bacteria forward. The motor ATPase PilB powers pilus assembly. Here we report the structures of the core ATPase domains of Geobacter metallireducens PilB bound to ADP and the non-hydrolysable ATP analogue, AMP-PNP, at 3.4 and 2.3 Å resolution, respectively. These structures reveal important differences in nucleotide binding between chains. Analysis of these differences reveals the sequential turnover of nucleotide, and the corresponding domain movements...
May 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/28472266/toward-establishing-minimum-requirements-for-extracellular-electron-transfer-in-geobacter-sulfurreducens
#8
Toshiyuki Ueki, Laurie N DiDonato, Derek R Lovley
The highly redundant pathways for extracellular electron transfer in Geobacter sulfurreducens must be simplified for this microorganism to serve as an effective chassis for applications such as the development of sensors and biocomputing. Five homologs of the periplasmic c-type cytochromes, PpcA-E, offer the possibility of multiple routes of electron transfer across the periplasm. The presence of a large number of outer membrane c-type cytochromes allows G. sulfurreducens to adapt to disruption of an electron transfer pathway in the outer membrane...
May 1, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28456041/enhanced-treatment-of-fischer-tropsch-wastewater-using-up-flow-anaerobic-sludge-blanket-system-coupled-with-micro-electrolysis-cell-a-pilot-scale-study
#9
Dexin Wang, Yuxing Han, Hongjun Han, Kun Li, Chunyan Xu
The coupling of micro-electrolysis cell (MEC) with an up-flow anaerobic sludge blanket (UASB) system in pilot scale was established for enhanced treatment of Fischer-Tropsch (F-T) wastewater. The lowest influent pH (4.99±0.10) and reduced alkali addition were accomplished under the assistance of anaerobic effluent recycling of 200% (stage 5). Simultaneously, the optimum COD removal efficiency (93.5±1.6%) and methane production (2.01±0.13m(3)/m(3)·d) at the lower hydraulic retention time (HRT) were achieved in this stage...
April 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28452369/corrigendum-mechanistic-stratification-in-electroactive-biofilms-of-geobacter-sulfurreducens-mediated-by-pilus-nanowires
#10
Rebecca J Steidl, Sanela Lampa-Pastirk, Gemma Reguera
No abstract text is available yet for this article.
April 28, 2017: Nature Communications
https://www.readbyqxmd.com/read/28443365/enhanced-phosphorus-reduction-in-simulated-eutrophic-water-a-comparative-study-of-submerged-macrophytes-sediment-microbial-fuel-cells-and-their-combination
#11
Peng Xu, Enrong Xiao, Dan Xu, Juan Li, Yi Zhang, Zhigang Dai, Qiaohong Zhou, Zhenbin Wu
The phosphorus content reduction in overlying and sediment pore water was attempted by integrating sediment microbial fuel cells (SMFCs) with the submerged macrophyte Vallisneria spiralis. A comparative study was conducted to treat simulated water rich in phosphate with a control and three treatments: SMFC alone (SMFC), submerged macrophytes alone (macophyte), and combined macrophytes and fuel cells (M-SMFC). All treatments increased sediment Eh and pH of the overlying water, thus promoting phosphorus flux from water column to sediments...
April 26, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28442658/predominant-but-previously-overlooked-prokaryotic-drivers-of-reductive-nitrogen-transformation-in-paddy-soils-revealed-by-metatranscriptomics
#12
Yoko Masuda, Hideomi Itoh, Yutaka Shiratori, Kazuo Isobe, Shigeto Otsuka, Keishi Senoo
Waterlogged paddy soils possess anoxic zones in which microbes actively induce reductive nitrogen transformation (RNT). In the present study, a shotgun RNA sequencing analysis (metatranscriptomics) of paddy soil samples revealed that most RNT gene transcripts in paddy soils were derived from Deltaproteobacteria, particularly the genera Anaeromyxobacter and Geobacter. Despite the frequent detection of the rRNA of these microbes in paddy soils, their RNT-associated genes have rarely been identified in previous PCR-based studies...
April 22, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28431359/reconstruction-of-microbial-community-structures-as-evidences-for-soil-redox-coupled-reductive-dechlorination-of-pcp-in-a-mangrove-soil
#13
Yan Xu, Yan He, Xianjin Tang, Philip C Brookes, Jianming Xu
The aim was to investigate the influence of pentachlorophenol (PCP) on the soil microbial communities and the coupled mechanism between PCP reductive dechlorination and soil redox under anaerobic condition. Accordingly, a slurry incubation experiment was carried out in which bacterial and archaeal communities were detected by MiSeq amplicon sequencing. The original microbial community balance was gradually disrupted and new microbial structure was reconstructed subsequently through self-regulation and acclimation during PCP transformation, coupling with the changes of soil biogeochemical redox dynamics...
April 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28429471/formation-of-silver-nanoparticles-by-electron-transfer-in-peptides-and-c-cytochromes
#14
Serhii I Vasylevskyi, Sonja Kracht, Paula Corcosa, Katharina M Fromm, Bernd Giese, Michael Füeg
The reduction of Ag(+) ions to Ag(0) atoms is a highly endergonic reaction step, only the aggregation to Agn clusters leads to an exergonic process. These elementary chemical reactions play a decisive role if Ag nanoparticles (AgNPs) are generated by electron transfer (ET) reactions to Ag(+) ions. We studied the formation of AgNPs in peptides by photoinduced ET, and in c-cytochromes by ET from their Fe(2+) /hemes. Our earlier photoinduced experiments in peptides had demonstrated that histidine prevents AgNP formation...
April 21, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28402361/electronic-characterization-of-geobacter-sulfurreducens-pilins-in-self-assembled-monolayers-unmasks-tunnelling-and-hopping-conduction-pathways
#15
Krista M Cosert, Rebecca J Steidl, Angelines Castro-Forero, Robert M Worden, Gemma Reguera
The metal-reducing bacterium Geobacter sulfurreducens produces protein nanowires (pili) for fast discharge of respiratory electrons to extracellular electron acceptors such as iron oxides and uranium. Charge transport along the pili requires aromatic residues, which cluster once the peptide subunits (pilins) assemble keeping inter-aromic distances and geometries optimal for multistep hopping. The presence of intramolecular aromatic contacts and the predominantly α-helical conformation of the pilins has been proposed to contribute to charge transport and rectification...
April 12, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28401700/silica-immobilization-of-geobacter-sulfurreducens-for-constructing-ready-to-use-artificial-bioelectrodes
#16
Marta Estevez-Canales, David Pinto, Thibaud Coradin, Christel Laberty-Robert, Abraham Esteve-Núñez
Microbial electrochemical technologies (METs) rely on the control of interactions between microorganisms and electronic devices, enabling to transform chemical energy into electricity. We report a new approach to construct ready-to-use artificial bioelectrodes by immobilizing Geobacter sulfurreducens cells in composite materials associating silica gel and carbon felt fibres. Viability test confirmed that the majority of bacteria (ca. 70 ± 5%) survived the encapsulation process in silica and that cell density did not increase in 96 h...
April 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28391101/control-of-vocs-from-printing-press-air-emissions-by-anaerobic-bioscrubber-performance-and-microbial-community-of-an-on-site-pilot-unit
#17
D Bravo, P Ferrero, J M Penya-Roja, F J Álvarez-Hornos, C Gabaldón
A novel process consisted of an anaerobic bioscrubber was studied at the field scale for the removal of volatile organic compounds (VOCs) emitted from a printing press facility. The pilot unit worked under high fluctuating waste gas emissions containing ethanol, ethyl acetate, and 1-ethoxy-2-propanol as main pollutants, with airflows ranging between 184 and 1253 m(3) h(-1) and an average concentration of 1126 ± 470 mg-C Nm(-3). Three scrubber configurations (cross-flow and vertical-flow packings and spray tower) were tested, and cross-flow packing was found to be the best one...
April 6, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28377068/solution-structure-and-dynamics-of-the-outer-membrane-cytochrome-omcf-from-geobacter-sulfurreducens
#18
Joana M Dantas, Marta A Silva, David Pantoja-Uceda, David L Turner, Marta Bruix, Carlos A Salgueiro
Gene knock-out studies on Geobacter sulfurreducens cells showed that the outer membrane-associated monoheme cytochrome OmcF is involved in respiratory pathways leading to the extracellular reduction of Fe(III) and U(VI). In addition, microarray analysis of an OmcF-deficient mutant revealed that many of the genes with decreased transcript level were those whose expression is up-regulated in cells grown with a graphite electrode as electron acceptor, suggesting that OmcF also regulates the electron transfer to electrode surfaces and the concomitant electricity production by G...
April 1, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28374997/ecological-and-transcriptional-responses-of-anode-respiring-communities-to-nitrate-in-a-microbial-fuel-cell
#19
Varun N Srinivasan, Caitlyn S Butler
A poorly understood phenomenon with a potentially significant impact on electron recovery is competition in microbial fuel cells (MFC) between anode-respiring bacteria and microorganisms that use other electron acceptors. Nitrate is a constituent of different wastewaters and can act as a competing electron acceptor in the anode. Studies investigating the impact of competition on population dynamics in mixed communities in the anode are lacking. Here, we investigated the impact of nitrate at different C/N ratios of 1...
April 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28362850/chemical-property-based-sequence-characterization-of-ppca-and-its-homolog-proteins-ppcb-e-a-mathematical-approach
#20
Jayanta Kumar Das, Pabitra Pal Choudhury
Periplasmic c7 type cytochrome A (PpcA) protein is determined in Geobacter sulfurreducens along with its other four homologs (PpcB-E). From the crystal structure viewpoint the observation emerges that PpcA protein can bind with Deoxycholate (DXCA), while its other homologs do not. But it is yet to be established with certainty the reason behind this from primary protein sequence information. This study is primarily based on primary protein sequence analysis through the chemical basis of embedded amino acids...
2017: PloS One
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