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https://www.readbyqxmd.com/read/29668140/-standardization-of-a-multiplex-real-time-pcr-test-for-the-identification-of-angiostrongylus-cantonensis-a-costaricensis-and-a-vasorum
#1
Rubén E Varela-M, Jinney Stefany Arias, Luz Elena Velásquez
INTRODUCTION: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum. Clinical forms of the disease in humans are eosinophilic meningitis and abdominal angiostrongyliasis, while the most common effect on dogs are cardiopulmonary damages. It is deemed as an emerging disease as the result of the global dissemination of the African snail Lissachatina fulica, an intermediary host of these parasites...
March 15, 2018: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29668136/-a-fatal-case-series-of-rocky-mountain-spotted-fever-in-sonora-m%C3%A3-xico
#2
Jesús Delgado-De la Mora, Jesús David Licona-Enríquez, Marcia Leyva-Gastélum, David Delgado-De la Mora, Adela Rascón-Alcantar, Gerardo Álvarez-Hernández
INTRODUCTION: Rocky Mountain spotted fever is a highly lethal infectious disease, particularly if specific treatment with doxycycline is given belatedly. OBJECTIVE: To describe the clinical profile of fatal Rocky Mountain spotted fever cases in hospitalized patients in the state of Sonora, México. MATERIALS AND METHODS: We conducted a cross-sectional study on a series of 47 deaths caused by Rickettsia rickettsii from 2013 to 2016. The diagnosis of Rocky Mountain spotted fever was confirmed in a single blood sample by polymerase chain reaction (PCR) or by a four-fold increase in immunoglobulin G measured in paired samples analyzed by indirect immunofluorescence...
March 15, 2018: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29668135/-costs-of-chagas-disease-screening-test-in-blood-donors-in-two-colombian-blood-banks-2015
#3
Nelson José Alvis, Diana Patricia Díaz, Liliana Castillo, Nelson Rafael Alvis, María Isabel Bermúdez, Olga Maritza Berrío, Mauricio Beltrán, Carlos Andrés Castañeda-Orjuela
INTRODUCTION: Transfusion is a mechanism of transmission of Chagas' disease. There are no studies on the costs of the screening test in Colombian blood banks. OBJECTIVE: To estimate the costs of the screening test for Chagas' disease among blood donors in two Colombian blood banks, 2015. MATERIALS AND METHODS: We conducted a micro-costing study from the perspective of the health care provider to estimate the cost of Chagas' disease testing in two blood banks, Banco de Sangre de la Cruz Roja, Seccional Bolívar, and Banco de Sangre del Hospital de Yopal, Casanare, taking into account four cost categories: 1) Administrative costs: public services and insurance costs were calculated based on the blood bank area in square meters; 2) capital costs: building and equipment costs that were annualized using a 3% discount rate and a lifespan of 20 years for building and five for equipment; 3) costs of Chagas' disease test materials and reagents adjusted by blood bank production level, and 4) costs of staff in charge of Chagas' disease test processing...
March 15, 2018: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/29668113/molecular-dynamics-analysis-of-charge-transport-in-ionic-liquid-electrolytes-containing-added-salt-with-mono-di-and-trivalent-metal-cations
#4
José Manuel Vicent-Luna, Eneko Azaceta, Said Hamad, José Manuel Ortiz-Roldán, Ramón Tena-Zaera, Sofía Calero, Juan Antonio Anta
Among many other applications, room-temperature ionic liquids (ILs) are used as electrolytes for storage and energy-conversion devices. In this work, we investigate, at the microscopic level, the structural and dynamical properties of 1-methyl-1-butyl-pyrrolidinium bis(trifluoromethanesulfonyl) imide [C4 PYR]+ [Tf2 N]- IL-based electrolytes for metal-ion batteries. We carried out molecular dynamics simulations of electrolytes mainly composed of [C4 PYR]+ [Tf2 N]- IL with the addition of Mn+ -[Tf2 N]- metal salts (M=Li+ , Na+ , Ni2+ , Zn2+ , Co2+ , Cd2+ , and Al3+ , n=1, 2, and 3) dissolved in the IL...
April 18, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29668073/sequential-systematic-anti-mold-prophylaxis-with-micafungin-and-voriconazole-results-in-very-low-incidence-of-invasive-mold-infections-in-patients-undergoing-allogeneic-hematopoietic-stem-cell-transplantation
#5
Claudia Rosillo, Ana Maria Avila, Yao-Ting Huang, Sean Devlin, Christina Cho, Juan Montoro, Molly A Maloy, Genovefa A Papanicolaou, Pere Barba, Miguel-Angel Perales
BACKGROUND AND OBJECTIVES: Recipients of allogeneic hematopoietic stem cell transplantation (allo-HSCT) are at high risk for invasive mold infections (IMI). The goal of the study is to describe the incidence and outcome of IMI in patients after allo-HSCT in a large cohort of patients receiving anti-mold prophylaxis. METHODS: We conducted a retrospective review of 988 consecutive adults who underwent allo-HSCT in our center from 2008 through 2014. Standard prophylaxis consisted of micafungin 150mg IV daily from admission to day +7 +/- 3 followed by voriconazole until day +75 to +100...
April 18, 2018: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/29668070/physical-activity-and-sedentary-behaviour-in-a-flexible-office-based-workplace-employee-perceptions-and-priorities-for-change
#6
Heidi M Olsen, Wendy J Brown, Tracy Kolbe-Alexander, Nicola W Burton
ISSUE ADDRESSED: Many Australian employees now regularly work from home in some capacity. This new way of working has not been widely studied in relation to the potential implications for employees' health-related behaviour or workplace health promotion. The aim of this study was to explore office-based employees' perceptions of the impact of flexible work on physical activity and sedentary behaviour; and preferences for associated interventions. METHODS: Three focus groups were conducted with office-based employees (n=28) six months after the introduction of a flexible work policy...
April 18, 2018: Health Promotion Journal of Australia
https://www.readbyqxmd.com/read/29668047/miniaturization-of-the-microsuspension-salmonella-microsome-assay-in-agar-microplates
#7
José Ricardo R M Zwarg, Daniel A Morales, Bianca S Maselli, Werner Brack, Gisela A Umbuzeiro
The Salmonella/microsome assay (Ames test) is the most widely used mutagenicity test for the evaluation of pure chemicals and environmental samples. There are several versions of protocols available in the literature, including those that reduce the amount of sample needed for testing with liquid and agar media. The microsuspension version of the Salmonella/microsome assay is more sensitive than the standard protocol. It is performed using 5-times concentrated bacteria and less sample and S9 mixture, but still uses conventional Petri dishes (90 × 15 mm)...
April 18, 2018: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29668037/treatment-of-chronic-neurogenic-cough-with-in-office-superior-laryngeal-nerve-block
#8
C Blake Simpson, Kathleen M Tibbetts, Michael J Loochtan, Laura M Dominguez
OBJECTIVES: Neurogenic cough is believed to result from a sensory neuropathy involving the internal branch of the superior laryngeal nerve (SLN). We present our outcomes for the treatment of neurogenic cough with localized blockade of the internal branch of the SLN. METHODS: A retrospective chart review of patients who underwent in-office percutaneous SLN block for treatment of neurogenic cough between 2015 and 2017 was conducted. Patient demographics, indications for injection, and response to treatment were recorded and analyzed...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29667897/narrative-roles-among-contact-versus-noncontact-sexual-offenders
#9
Emma Hamilton, Delida Sanchez
The current study explored narrative roles among individuals convicted of a sexual offense. Narrative roles in a criminal context are defined as specific personal accounts utilized by offenders to justify illegal behavior. The chosen theoretical framework recognized four primary offender roles: Revengeful Mission/Romantic Quest, Professional, Victim, and Tragic Hero. A total of 23 interviews were conducted with individuals convicted of a sexual offense ( n = 11 contact, n = 12 noncontact) to explore and compare narrative roles between contact and noncontact offenders...
April 1, 2018: Sexual Abuse: a Journal of Research and Treatment
https://www.readbyqxmd.com/read/29667892/ct-colonography-performance-for-the-detection-of-polyps-and-cancer-in-adults-%C3%A2-65-years-old-systematic-review-and-meta-analysis
#10
Perry J Pickhardt, Loredana Correale, Silvia Delsanto, Daniele Regge, Cesare Hassan
OBJECTIVE: We sought to perform a systematic review and meta-analysis of published CT colonography (CTC) studies assessing lesion detection in senior-age (≥ 65 years old) cohorts. MATERIALS AND METHODS: We conducted a systematic review of CTC studies published between January 1994 and August 2017 containing data on senior-age adults. The primary endpoint was the CTC-positive rate for large colorectal polyps (≥ 10 mm) and masses. Secondary endpoints included lesions ≥ 6 mm, proven advanced neoplasia, and colorectal cancer (CRC)...
April 18, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29667878/safety-challenges-and-improvement-strategies-of-ethnic-minority-construction-workers-a-case-study-in-hong-kong
#11
Chunlin Wu, Xiaowei Luo, Tao Wang, Yue Wang, Bibek Sapkota
Due to cultural differences, ethnic minority construction workers are more difficult to manage and more vulnerable to accidents. This study aims to identify the major barriers faced by ethnic minority workers from their own perspectives and determine potential strategies to enhance safety climate of construction projects, thus ultimately improve their safety performance. A survey with modified Nordic safety climate questionnaire was conducted in a certain sub-contractor in Hong Kong. In-depth interviews, status quo description, major challenge investigation and safety knowledge tests were carried as well...
April 18, 2018: International Journal of Occupational Safety and Ergonomics: JOSE
https://www.readbyqxmd.com/read/29667870/focus-is-key-panic-focused-interpretations-are-associated-with-symptomatic-improvement-in-panic-focused-psychodynamic-psychotherapy
#12
John R Keefe, Nili Solomonov, Robert J Derubeis, Alexander C Phillips, Fredric N Busch, Jacques P Barber, Dianne L Chambless, Barbara L Milrod
OBJECTIVE: This study examines whether, in panic-focused psychodynamic psychotherapy (PFPP), interpretations of conflicts that underlie anxiety (panic-focused or PF-interpretations) are specifically associated with subsequent panic disorder (PD) symptom improvement, over and above the provision of non-symptom-focused interpretations. METHOD: Technique use in Sessions 2 and 10 of a 24-session PFPP protocol was assessed for the 65 patients with complete outcome data randomized to PFPP in a two-site trial of psychotherapies for PD...
April 18, 2018: Psychotherapy Research: Journal of the Society for Psychotherapy Research
https://www.readbyqxmd.com/read/29667836/molecular-dynamics-simulations-are-redefining-our-view-of-peptides-interacting-with-biological-membranes
#13
Jakob P Ulmschneider, Martin B Ulmschneider
Ever since the first molecular mechanics computer simulations of biological molecules became possible, there has been the dream to study all complex biological phenomena in silico, simply bypassing the enormous experimental challenges and their associated costs. For this, two inherent requirements need to be met: First, the time scales achievable in simulations must reach up to the millisecond range and even longer. Second, the computational model must accurately reproduce what is measured experimentally. Despite some recent successes, the general consensus in the field to date has been that neither of these conditions have yet been met and that the dream will be realized, if at all, only in the distant future...
April 18, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29667818/balancing-the-electron-and-hole-transfer-for-efficient-quantum-dot-light-emitting-diodes-by-employing-a-versatile-organic-electron-blocking-layer
#14
Xiao Jin, Chun Chang, Weifeng Zhao, Shujuan Huang, Xiaobing Gu, Qin Zhang, Feng Li, Yubao Zhang, Qinghua Li
The electron blocking layer (EBL) is important to balance the charge carrier transfer and achieve highly efficient quantum dot light-emitting diodes (QLEDs). Here we report the utilization of a soluble tert-butyldimethylsilyl chloride-modified poly(p-phenylene benzobisoxazole) (TBS-PBO) as an EBL for simultaneous good charge carrier transfer balance while maintaining high current density. We show that the versatile TBS-PBO blocks excess electron injection into the QDs, thus leading to better charge carrier transfer balance...
April 18, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29667775/views-of-parents-of-children-with-sickle-cell-disease-on-pre-implantation-genetic-diagnosis
#15
Isha Darbari, Jeanne E O'Brien, Steven J Hardy, Barbara Speller-Brown, Lisa Thaniel, Brenda Martin, Deepika S Darbari, Robert S Nickel
Pre-implantation genetic diagnosis (PGD) is an option for parents who have a child with sickle cell disease (SCD) to have another child without SCD. We conducted a survey of 19 parents with at least one child with SCD to investigate views on PGD. Before education, 44% of parents were aware of PGD. All parents rated PGD education as important. All parents considering another child also reported interest in using PGD if insurance covered its costs. Parents who have a child with SCD appear to be interested in PGD and educational tools informing this group about PGD should be developed...
April 18, 2018: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/29667772/tead4-exerts-pro-metastatic-effects-and-is-negatively-regulated-by-mir6839-3p-in-lung-adenocarcinoma-progression
#16
Qun Zhang, Hang Fan, Qian Zou, Hongda Liu, Bing Wan, Suhua Zhu, Yangbo Hu, Huijuan Li, ChenXi Zhang, Li Zhou, Qingqing Zhu, Kunhong Xiao, Jianya Zhang, Ping Zhan, Tangfeng Lv, Yong Song
Several studies have shown the tumorigenesis role of transcriptional enhancer associate domain (TEAD) proteins; here, we initially explored expression, function and signalling mechanisms of TEAD4 in lung adenocarcinoma (LAD). Both the mRNA and protein levels of TEAD4 were increased in LAD tissues than those in adjacent nontumourous tissues. Besides, database search indicated a poorer clinical outcome in LAD patients with higher TEAD4 expression, revealing its potential tumour-promoting role. Therefore, we conducted cellular experiments to further investigate its effect on tumour phenotypes...
April 18, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29667764/rrm2-promotes-the-progression-of-human-glioblastoma
#17
Chao Li, Jingfang Zheng, Si Chen, Bin Huang, Guosheng Li, Zichao Feng, Jiwei Wang, Shujun Xu
Glioblastoma pathogenesis is related to multiple processes that affected by dozens of regulatory factors, but the potential underlying factors regulating glioblastoma progression remains unclear. The goal of this research was to determine how the ribonucleotide reductase M2 subunit (RRM2) influenced proliferation, invasion, migration, and apoptosis of human glioblastoma cells. The level of proliferation of human glioblastoma cells was measured through CCK8, colony formation assay and immunofluorescence stains...
April 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29667754/estrogen-regulated-expression-of-p450arom-genes-in-the-brain-and-ovaries-of-adult-female-indian-climbing-perch-anabas-testudineus
#18
Puja Pal, Sujata Roy Moulik, Shreyasi Gupta, Payel Guha, Suravi Majumder, Sourav Kundu, Buddhadev Mallick, Kousik Pramanick, Dilip Mukherjee
Cytochrome P450arom (CYP19), a product of cyp19a1 gene, catalyzes the conversion of androgens to estrogens and is essential for regulation of reproductive function in vertebrates. In the present study, we isolated partial cDNA encoding the ovarian (cyp19a1a) and brain (cyp19a1b) P450arom genes from adult female perch, Anabas testudineus and investigated their regulation by estrogen in vivo. Results demonstrated that cyp19a1a and cyp19a1b predominate in ovary and brain respectively, with quantity of both attuned to reproductive cycle...
April 18, 2018: Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
https://www.readbyqxmd.com/read/29667742/genetic-association-and-expression-analyses-of-the-phosphatidylinositol-4-phosphate-5-kinase-pip5k1c-gene-in-alcohol-use-disorder-relevance-for-pain-signaling-and-alcohol-use
#19
Ji Soo Lee, Jill L Sorcher, Allison D Rosen, Ruslan Damadzic, Hui Sun, Melanie Schwandt, Markus Heilig, John Kelly, Kelsey L Mauro, Audrey Luo, Daniel Rosoff, Christine Muench, Jeesun Jung, Zachary A Kaminsky, Falk W Lohoff
BACKGROUND: The gene encoding Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K1C) has been recently implicated in pain regulation. Interestingly, a recent cross-tissue and cross-phenotypic epigenetic analysis identified the same gene in alcohol use disorder (AUD). Given the high comorbidity between AUD and chronic pain, we hypothesized that genetic variation in PIP5K1C might contribute to susceptibility to AUD. METHODS: We conducted a case-control association study of genetic variants in PIP5K1C...
April 18, 2018: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29667729/hla-dqb1-03-genotype-and-perioperative-blood-transfusion-are-not-conducive-to-the-prognosis-of-patients-with-gastric-cancer
#20
Shen-Kang Zhou, Lei-Lei Yang, Rui Chen, Yong Lu, Yong-Hua Zheng
BACKGROUND: Gastric cancer (GC) is a disease associated with a higher incidence and mortality, and some host genetic polymorphisms have been reported as potential factors contributing to the development of GC. In view of this, the study was conducted to investigate the effects of HLA-DQB1 gene polymorphisms and perioperative blood transfusion on prognosis of patients with gastric cancer (GC). METHODS: A total of 142 patients with GC (case group) and 150 healthy controls (control group) were enrolled...
April 18, 2018: Journal of Clinical Laboratory Analysis
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