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Sylvia M Bardet, Lynn Carr, Malak Soueid, Delia Arnaud-Cormos, Philippe Leveque, Rodney P O'Connor
Despite the biomedical advances of the last century, many cancers including glioblastoma are still resistant to existing therapies leaving patients with poor prognoses. Nanosecond pulsed electric fields (nsPEF) are a promising technology for the treatment of cancer that have thus far been evaluated in vitro and in superficial malignancies. In this paper, we develop a tumor organoid model of glioblastoma and apply intravital multiphoton microscopy to assess their response to nsPEFs. We demonstrate for the first time that a single 10 ns, high voltage electric pulse (35-45 kV/cm), collapses the perfusion of neovasculature, and also alters the diameter of capillaries and larger vessels in normal tissue...
October 4, 2016: Scientific Reports
Jinsong Guo, Yu Wang, Jing Wang, Jue Zhang, Jing Fang
Nanosecond pulsed electric fields (nsPEFs) are a non-thermal and non-toxic technology that induce a myriad of biological effects. They have been proven to be effective in tumor shrinkage, but few studies focus on its radiosensitization in oral tongue squamous cell carcinoma. The purpose of this research was to study the radiosensitization effect of nsPEFs on a human oral tongue cancer cell line Tca8113 and to investigate the potential antitumor mechanism. A Tca8113 cell line was tested respectively by MTT assay, clonogenic assay, flow cytometry assay, annexin V-FITC/PI assay, mitochondrial potential assay and total nitric oxide assay...
September 22, 2016: Bioelectrochemistry
Wei Lu, Ke Wu, Xiangjun Hu, Xiangdong Xie, Jing Ning, Changzhen Wang, Hongmei Zhou, Guoshan Yang
PURPOSE: Intracellular electroporation occurs when the cells are exposed to nanosecond pulsed electric field (nsPEF). It is believed the electroporation (formation and extension of pores on the membrane induced by external electric field) is affected significantly by the transmembrane potential. This paper analyzed transmembrane potential induced by nsPEF in the term of pulse frequency spectrum, aiming to provide a theoretical explanation to intracellular bio-effects. METHODS: Based on the double-shelled spherical cell model, the frequency dependence of transmembrane potential was obtained by solving Laplace's equation, while the time course of transmembrane potential was obtained by a method combined with discrete Fourier transform and Laplace transform...
October 10, 2016: International Journal of Radiation Biology
Agnese Denzi, Elena Della Valle, Francesca Apollonio, Marie Breton, Lluis M Mir, Micaela Liberti
Smart drug delivery systems represent an interesting tool to significantly improve the efficiency and the precision in the treatment of a broad category of diseases. In this context, a drug delivery mediated by nanosecond pulsed electric fields seems a promising technique, allowing for a controlled release and uptake of drugs by the synergy between the electropulsation and nanocarriers with encapsulated drugs. The main concern about the use of electroporation for drug delivery applications is the difference in dimension between the liposome (nanometer range) and the cell (micrometer range)...
August 25, 2016: Journal of Membrane Biology
Erick K Moen, Bennett L Ibey, Hope T Beier, Andrea M Armani
Plasma membrane disruption can trigger a host of cellular activities. One commonly observed type of disruption is pore formation. Molecular dynamic (MD) simulations of simplified lipid membrane structures predict that controllably disrupting the membrane via nano-scale poration may be possible with nanosecond pulsed electric fields (nsPEF). Until recently, researchers hoping to verify this hypothesis experimentally have been limited to measuring the relatively slow process of fluorescent markers diffusing across the membrane, which is indirect evidence of nanoporation that could be channel-mediated...
August 14, 2016: Biochimica et Biophysica Acta
Anna Steuer, Anke Schmidt, Petra Labohá, Pavel Babica, Juergen F Kolb
Gap junctional intercellular communication (GJIC) is an important mechanism that is involved and affected in many diseases and injuries. So far, the effect of nanosecond pulsed electric fields (nsPEFs) on the communication between cells was not investigated. An in vitro approach is presented with rat liver epithelial WB-F344 cells grown and exposed in a monolayer. In order to observe sub-lethal effects, cells were exposed to pulsed electric fields with a duration of 100ns and amplitudes between 10 and 20kV/cm...
December 2016: Bioelectrochemistry
Esin B Sözer, Yu-Hsuan Wu, Stefania Romeo, P Thomas Vernier
High-intensity nanosecond pulsed electric fields (nsPEFs) permeabilize cell membranes. Although progress has been made toward an understanding of the mechanism of nsPEF-induced membrane poration, the dependence of pore size and distribution on pulse duration, strength, number, and repetition rate remains poorly defined experimentally. In this paper, we characterize the size of nsPEF-induced pores in living cell membranes by isosmotically replacing the solutes in pulsing media with polyethylene glycols and sugars before exposing Jurkat T lymphoblasts to 5 ns, 10 MV/m electric pulses...
July 19, 2016: Journal of Membrane Biology
Jody C Cantu, Melissa Tarango, Hope T Beier, Bennett L Ibey
Previous work from our laboratory demonstrated nanopore formation in cell membranes following exposure to nanosecond pulsed electric fields (nsPEF). We observed differences in sensitivity to nsPEF in both acute membrane injury and 24 h lethality across multiple cells lines. Based on these data, we hypothesize that the biological response of cells to nsPEF is dependent on the physical properties of the plasma membrane (PM), including regional cholesterol content. Results presented in this paper show that depletion of membrane cholesterol disrupts the PM and increases the permeability of cells to small molecules, including propidium iodide and calcium occurring after fewer nsPEF...
July 15, 2016: Biochimica et Biophysica Acta
Shengyong Yin, Xinhua Chen, Haiyang Xie, Lin Zhou, Danjing Guo, Yuning Xu, Liming Wu, Shusen Zheng
Previous studies showed nanosecond pulsed electric field (nsPEF) can ablate solid tumors including hepatocellular carcinoma (HCC) but its effect on cell membrane is not fully understood. We hypothesized nsPEF disrupt the microdomains on outer-cellular membrane with direct mechanical force and as a result the plasma membrane permeability increases to facilitate the small molecule intake. Three HCC cells were pulsed one pulse per minute, an interval longer than nanopore resealing time. The cationized ferritin was used to mark up the electronegative microdomains, propidium iodide (PI) for membrane permeabilization, energy dispersive X-ray spectroscopy (EDS) for the negative cell surface charge and cisplatin for inner-cellular cytotoxicity...
August 15, 2016: Experimental Cell Research
Jinsong Guo, Ruonan Ma, Bo Su, Yinglong Li, Jue Zhang, Jing Fang
Avermectins, a group of anthelmintic and insecticidal agents produced from Streptomyces avermitilis, are widely used in agricultural, veterinary, and medical fields. This study presents the first report on the potential of using nanosecond pulsed electric fields (nsPEFs) to improve avermectin production in S. avermitilis. The results of colony forming units showed that 20 pulses of nsPEFs at 10 kV/cm and 20 kV/cm had a significant effect on proliferation, while 100 pulses of nsPEFs at 30 kV/cm exhibited an obvious effect on inhibition of agents...
2016: Scientific Reports
Csaba Orbán, Esther Pérez-García, Anna Bajnok, Gethin McBean, Gergely Toldi, Alfonso Blanco-Fernandez
Nanosecond pulsed electric field (nsPEF) is a novel method to increase cell proliferation rate. The phenomenon is based on the microporation of cellular organelles and membranes. However, we have limited information on the effects of nsPEF on cell physiology. Several studies have attempted to describe the effects of this process, however no real time measurements have been conducted to date. In this study we designed a model system which allows the measurement of cellular processes before, during and after nsPEF treatment in real time...
May 2016: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Gary L Thompson, Caleb C Roth, Marjorie A Kuipers, Gleb P Tolstykh, Hope T Beier, Bennett L Ibey
Permeabilization of cell membranes occurs upon exposure to a threshold absorbed dose (AD) of nanosecond pulsed electric fields (nsPEF). The ultimate, physiological bioeffect of this exposure depends on the type of cultured cell and environment, indicating that cell-specific pathways and structures are stimulated. Here we investigate 10 and 600 ns duration PEF effects on Chinese hamster ovary (CHO) cell nuclei, where our hypothesis is that pulse disruption of the nuclear envelope membrane leads to observed cell death and decreased viability 24 h post-exposure...
January 29, 2016: Biochemical and Biophysical Research Communications
Fei Xie, Frency Varghese, Andrei G Pakhomov, Iurii Semenov, Shu Xiao, Jonathan Philpott, Christian Zemlin
BACKGROUND: Ablation of cardiac tissue is an essential tool for the treatment of arrhythmias, particularly of atrial fibrillation, atrial flutter, and ventricular tachycardia. Current ablation technologies suffer from substantial recurrence rates, thermal side effects, and long procedure times. We demonstrate that ablation with nanosecond pulsed electric fields (nsPEFs) can potentially overcome these limitations. METHODS: We used optical mapping to monitor electrical activity in Langendorff-perfused New Zealand rabbit hearts (n = 12)...
2015: PloS One
Richard Nuccitelli, Jon Casey Berridge, Zachary Mallon, Mark Kreis, Brian Athos, Pamela Nuccitelli
We have used both a rat orthotopic hepatocellular carcinoma model and a mouse allograft tumor model to study liver tumor ablation with nanosecond pulsed electric fields (nsPEF). We confirm that nsPEF treatment triggers apoptosis in rat liver tumor cells as indicated by the appearance of cleaved caspase 3 and 9 within two hours after treatment. Furthermore we provide evidence that nsPEF treatment leads to the translocation of calreticulin (CRT) to the cell surface which is considered a damage-associated molecular pattern indicative of immunogenic cell death...
2015: PloS One
Barbara Hargrave, Francis Li
Platelet-rich plasma is a therapeutic strategy used for accelerating wound healing of a wide range of tissues through the release of platelet growth factors. Here, we describe a nonchemical, safe method for preparing platelet-rich plasma using nanosecond-pulsed electric fields (nsPEFs) and investigated the effect of this platelet-rich plasma on reperfusion of blood in large skin flap or ischemic hind limb wounds in New Zealand White rabbits. Laser Doppler images of blood flow to the dorsal surface of skin flap wounds or to ischemic hind limb wounds were obtained from wounds treated with 0...
July 2015: Physiological Reports
Xudong Miao, Shengyong Yin, Zhou Shao, Yi Zhang, Xinhua Chen
OBJECTIVE: Recent studies suggest that nanosecond pulsed electric field (nsPEF) is a novel minimal invasive and non-thermal ablation method that can induce apoptosis in different solid tumors. But the efficacy of nsPEF on bone-related tumors or bone metastasis is kept unknown. The current study investigates antitumor effect of nsPEF on osteosarcoma MG-63 cells in vitro. METHOD: MG-63 cells were treated with nsPEF with different electric field strengths (0, 10, 20, 30, 40, and 50 kV/cm) and different pulse numbers (0, 6, 12, 18, 24, and 30 pulses)...
2015: Journal of Orthopaedic Surgery and Research
Patrizia Lamberti, Stefania Romeo, Anna Sannino, Luigi Zeni, Olga Zeni
The impact of pulse repetition rate (PRR) in modulating electroporation (EP) induced by nanosecond pulsed electric fields (nsPEFs) in mammalian cells was approached here by performing both biological and numerical analysis. Plasma membrane permeabilization and viability of Jurkat cells were analyzed after exposure to 500, 1.3 MV/m, 40 ns PEFs with variable PRR (2-30 Hz). A finite-element model was used to investigate EP dynamics in a single cell under the same pulsing conditions, by looking at the time course of transmembrane voltage and pore density on the ns time scale...
September 2015: IEEE Transactions on Bio-medical Engineering
Diganta Dutta, Anthony Asmar, Michael Stacey
We investigated the effects of a single 60 nanosecond pulsed electric field (nsPEF) of low (15 kV/cm) and high (60 kV/cm) field strengths on cellular morphology and membrane elasticity in Jurkat cells using fluorescent microscopy and atomic force microscopy (AFM). We performed force displacement measurements on cells using AFM and calculated the Young's modulus for membrane elasticity. Differential effects were observed depending upon pulsing conditions. We found that a single nsPEF of low field strength did not induce any apparent cytoskeletal breakdown and had minor morphological changes...
May 2015: Micron: the International Research and Review Journal for Microscopy
Jody C Ullery, Melissa Tarango, Caleb C Roth, Bennett L Ibey
Previous work demonstrated significant changes in cellular membranes following exposure of cells to nanosecond pulsed electric fields (nsPEF), including nanoporation and increases in intracellular calcium concentration. While it is known that nsPEF exposure can cause cell death, how cells repair and survive nsPEF-induced cellular damage is not well understood. In this paper, we investigated whether autophagy is stimulated following nsPEF exposure to repair damaged membranes, proteins, and/or organelles in a pro-survival response...
March 6, 2015: Biochemical and Biophysical Research Communications
Shan Wu, Jinsong Guo, Wendong Wei, Jue Zhang, Jing Fang, Stephen J Beebe
BACKGROUND: Chemotherapy either before or after surgery is a common breast cancer treatment. Long-term, high dose treatments with chemotherapeutic drugs often result in undesirable side effects, frequent recurrences and resistances to therapy. METHODS: The anti-cancer drug, gemcitabine (GEM) was used in combination with pulse power technology with nanosecond pulsed electric fields (nsPEFs) for treatment of human breast cancer cells in vitro. Two strategies include sensitizing mammary tumor cells with GEM before nsPEF treatment or sensitizing cells with nsPEFs before GEM treatment...
2014: Cancer Cell International
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