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https://www.readbyqxmd.com/read/28445896/total-serum-antioxidant-capacity-in-healthy-normal-weight-and-asymptomatic-overweight-adults
#1
Ana Petelin, Paola Tedeschi, Annalisa Maietti, Mihaela Jurdana, Vincenzo Brandolini, Zala Jenko Pražnikar
Obesity and overweight are major contributors to the burden of chronic disease. Both are defined as abnormal or excessive fat accumulation and by increased production of free radicals leading to oxidative stress. The aim of the present study was to evaluate whether overweight and fat accumulation is associated with serum total antioxidant capacity (TAC) in men and women, irrespective of nutritional habits, nutrient intakes, physical activity, smoking, and other confounders, which may be responsible for modifying the association between serum TAC and overweight/obesity measures...
April 26, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28445868/suppressionof-pyruvate-dehydrogenase-kinase-2-re-sensitizes-paclitaxel-resistant-human-lung-cancer-cells-to-paclitaxel
#2
Hong Sun, Anyou Zhu, Xiang Zhou, Fengchao Wang
Despite impressive initial clinical responses, the majority of lung cancer patients treated with paclitaxel eventually develop resistance to the drug. Pyruvate dehydrogenase kinase-2 (PDK2) is a key regulator of glycolysis and oxidative phosphorylation, and its expression is increased in a variety of tumors. In this study, the role of PDK2 in mediating paclitaxel resistance in lung cancer cells was investigated using biochemical and isotopic tracing methods. Increased expression of PDK2 was observed in paclitaxel-resistant cells ascompared totheir parental cells...
April 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445825/physico-chemical-protection-rather-than-biochemical-composition-governs-the-responses-of-soil-organic-carbon-decomposition-to-nitrogen-addition-in-a-temperate-agroecosystem
#3
Wenbing Tan, Guoan Wang, Caihong Huang, Rutai Gao, Beidou Xi, Biao Zhu
The heterogeneous responses of soil organic carbon (SOC) decomposition in different soil fractions to nitrogen (N) addition remain elusive. In this study, turnover rates of SOC in different aggregate fractions were quantified based on changes in δ(13)C following the conversion of C3 to C4 vegetation in a temperate agroecosystem. The turnover of both total organic matter and specific organic compound classes within each aggregate fraction was inhibited by N addition. Moreover, the intensity of inhibition increases with decreasing aggregate size and increasing N addition level, but does not vary among chemical compound classes within each aggregate fraction...
April 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28445628/characterization-of-four-latin-american-families-confirms-previous-findings-and-reveals-novel-features-of-acid-labile-subunit-als-deficiency
#4
Paula A Scaglia, Ana C Keselman, Débora Braslavsky, Lucía C Martucci, Liliana M Karabatas, Sabina Domené, Mariana L Gutiérrez, María G Ballerini, María G Ropelato, Angela Spinola-Castro, Adriana A Siviero-Miachon, Juliana Saito Tartuci, Sol Rodríguez Azrak, Rodolfo A Rey, Héctor G Jasper, Ignacio Bergadá, Horacio M Domené
OBJECTIVE: ALS deficiency (ACLSD), caused by inactivating mutations in both IGFALS gene alleles, is characterized by marked reduction of IGF-I and IGFBP-3 levels associated to mild growth retardation. The aim of this study was to expand the known phenotype and genetic characteristics of ACLSD by reporting data from four index cases and their families. DESIGN: Auxological data, biochemical and genetic studies were performed in four children diagnosed with ACLSD and all available relatives...
April 26, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28445605/high-throughput-cell-mechanical-phenotyping-for-label-free-titration-assays-of-cytoskeletal-modifications
#5
Stefan Golfier, Philipp Rosendahl, Alexander Mietke, Maik Herbig, Jochen Guck, Oliver Otto
The mechanical fingerprint of cells is inherently linked to the structure of the cytoskeleton and can serve as a label free marker for cell homeostasis or pathologic states. How cytoskeletal composition affects the physical response of cells to external loads has been intensively studied with a spectrum of techniques, yet quantitative and statistically powerful investigations in the form of titration assays are hampered by the low throughput of most available methods. In this study, we employ real-time deformability cytometry (RT-DC), a novel microfluidic tool to examine the effects of biochemically modified F-actin and microtubule stability and nuclear chromatin structure on cell deformation in a human leukemia cell line (HL60)...
April 26, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28445490/the-effect-of-swimming-exercise-on-adenine-induced-kidney-disease-in-rats-and-the-influence-of-curcumin-or-lisinopril-thereon
#6
Badreldin H Ali, Turan Karaca, Yousuf Al Suleimani, Mohammed Al Za'abi, Jamila Al Kalbani, Mohammed Ashique, Abderrahim Nemmar
Patients with chronic kidney disease (CKD) have been reported to benefit from different types of exercises. It has also been shown that the ACE inhibitor lisinopril, and the natural product curcumin are also beneficial in different models of CKD in rats. We assessed the influence of moderate swimming exercise (SE) on rats with adenine-induced CKD, and tested the possible effects of lisinopril and/or curcumin thereon using several physiological, biochemical, histopathological and immunohistochemical parameters...
2017: PloS One
https://www.readbyqxmd.com/read/28445403/detection-of-hepatitis-b-virus-m204i-mutation-by-quantum-dot-labeled-dna-probe
#7
Cheng Zhang, Yiping Chen, Xinmiao Liang, Guanhua Zhang, Hong Ma, Leng Nie, Yu Wang
Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutations is important in clinical diagnosis. Therefore, we developed a sensitive, low-cost and convenient QDs-mediated fluorescent method for the detection of HBV gene mutations in real serum samples from chronic hepatitis B (CHB) patients who had received lamivudine or telbivudine antiviral therapy...
April 26, 2017: Sensors
https://www.readbyqxmd.com/read/28445311/an-observational-study-on-the-relationship-between-serum-uric-acid-and-hypertension-in-a-northern-chinese-population-aged-45-to-59-years
#8
Feng-Na Yu, Yun-Xia Shi, Hai-Ying Cheng, Xun-Lan Huang, Shan-Shan Liu
Little is known about the relationship serum uric acid (SUA) and hypertension in Chinese population. Therefore, the aim of this study was to determine the association between SUA and hypertension in a northern Chinese population. The participants were a group of 1730 Chinese adults aged 45 to 59 years in Shandong Province, who were recruited from the Linyi Nutrition and Health Survey (2015-2016). Hypertension was defined as systolic blood pressure ≥140 mm Hg and/or diastolic blood pressure ≥90 mm Hg...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28445300/malignant-phosphaturic-mesenchymal-tumor-with-pulmonary-metastasis-a-case-report
#9
Shui Qiu, Li-Li Cao, Yue Qiu, Ping Yan, Zi-Xuan Li, Jiang Du, Li-Mei Sun, Qing-Fu Zhang
RATIONALE: Phosphaturic mesenchymal tumor (PMT) is a new tumor entity of soft tissue and bone tumor recently accepted by the World Health Organization, which typically causes the paraneoplastic syndrome of tumor-induced osteomalacia (TIO). The majority of PMTs follow a benign clinical course and local recurrence occurs in < 10% of cases, malignant PMTs with distant organ metastasis are extremely uncommon. PATIENT CONCERNS: We reported a 41-year-old woman who was diagnosed with PMT 10 years ago with a repeated recurrence and pulmonary metastasis...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28444921/biochemical-characterization-and-analyses-of-polysialic-acid-associated-carrier-proteins-and-genes-in-piglets-during-neonatal-development
#10
Bing Wang, Yue Chen, He Ren, Nai Zhang, Frederic Arthur Troy Ii
Polysialic acid plays a key role in cancer metastasis and neurodevelopment. Our aim was to determine the developmental gene expression profiles for the two polysialyltransferases ST 8Sia II and ST8Sia IV, NCAM, SynCAM1, Neuropilin-2 (NRP2) and their polysialylated cognate glycans in different regions of the piglet brain during postnatal development. Our findings show that: (1) The cellular levels of mRNA coding for ST8Sia II and ST8Sia IV, NCAM, SynCAM1, NRP2 and polySia are age-dependent and cell type specific during neonatal brain development; (2) There was a lack of correlation between abundance level of mRNA coding for ST8Sia II and ST8Sia IV and the abundance level of the post-translation expression of polySia in all nine brain regions; (3) Expression levels of polySia did not correlate with the levels of the carrier proteins, NCAM-140, SynCAM1 and NRP2 in nine brain regions; (4) The cellular abundance of ST8Sia II and ST8Sia IV in nine subregions of piglet brain is regulated at the level of translation/posttranslation, and not at the level of transcription...
April 26, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28444798/multi-gel-casting-apparatus-for-vertical-polyacrylamide-gels-with-in-built-solution-flow-system-and-liquid-level-detectors
#11
Praveen Maurye, Arpita Basu, Tapas Kumar Bandyopadhyay, Jayanta Kumar Biswas, Bimal Prasana Mohanty
Polyacrylamide gel electrophoresis (PAGE) is the most widely used technique for the separation and biochemical analysis of biomolecules. The ever growing field of proteomics and genomics necessitates the analysis of many proteins and nucleic acid samples to understand further about the structure and function of cells. Simultaneous analysis of multiple protein samples often requires casting of many PAGE gels. Several variants of multi-gel casting/electrophoresis apparatuses are frequently used in research laboratories...
April 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28444797/the-transcription-factor-myb115-contributes-to-the-regulation-of-proanthocyanidin-biosynthesis-and-enhances-fungal-resistance-in-poplar
#12
Lijun Wang, Lingyu Ran, Yisu Hou, Qiaoyan Tian, Chaofeng Li, Rui Liu, Di Fan, Keming Luo
Proanthocyanidins (PAs) are major defense phenolic compounds in the leaves of poplar (Populus spp.) in response to abiotic and biotic stresses. Transcriptional regulation of PA biosynthetic genes by the MYB-basic helix-loop-helix (bHLH)-WD40 complexes in poplar is not still fully understood. Here, an Arabidopsis TT2-like gene MYB115 was isolated from Populus tomentosa and characterized by various molecular, genetic and biochemical approaches. MYB115 restored PA productions in the seed coat of the Arabidopsis tt2 mutant...
April 26, 2017: New Phytologist
https://www.readbyqxmd.com/read/28444736/selective-progesterone-receptor-modulators-sprms-for-uterine-fibroids
#13
REVIEW
Ally Murji, Lucy Whitaker, Tiffany L Chow, Mara L Sobel
BACKGROUND: Uterine fibroids are smooth muscle tumours arising from the uterus. These tumours, although benign, are commonly associated with abnormal uterine bleeding, bulk symptoms and reproductive dysfunction. The importance of progesterone in fibroid pathogenesis supports selective progesterone receptor modulators (SPRMs) as effective treatment. Both biochemical and clinical evidence suggests that SPRMs may reduce fibroid growth and ameliorate symptoms. SPRMs can cause unique histological changes to the endometrium that are not related to cancer, are not precancerous and have been found to be benign and reversible...
April 26, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28444734/highly-electronegative-low-density-lipoprotein-l5-evokes-microglial-activation-and-creates-a-neuroinflammatory-stress-via-toll-like-receptor-4-signaling
#14
Liang-En Yu, Chiou-Lian Lai, Ching-Tien Lee, Jiz-Yuh Wang
Atherogenic risk factors, such as hypercholesterolemia, are associated with increased risk of neurodegeneration, especially Alzheimer's dementia. Human plasma electronegative low-density lipoprotein [LDL(-)], especially L5, may serve as an important contributing factor. L5 promoting an inflammatory action in atherosclerosis has been extensively studied. However, the role of L5 in inducing neuroinflammation remains unknown. Here, we examined the impact of L5 on immune activation and cell viability in cultured BV-2 microglia...
April 26, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28444650/-newborn-screening-on-cystic-fibrosis-in-germany-comparison-of-the-new-screening-protocol-with-an-alternative-protocol
#15
Olaf Sommerburg, Mirjam Stahl, Jutta Hammermann, Jürgen G Okun, Andreas Kulozik, Georg Hoffmann, Marcus Mall
Background For the new cystic fibrosis (CF) newborn screening program in Germany the Federal Joint Committee (G-BA) implemented a new screening protocol using immunoreactive trypsinogen (IRT) as first and pancreatitis associated protein (PAP) as second tier. Gene analysis with a panel of 31 CFTR-mutations is used as third tier to increase the positive predictive value (PPV) which is known to be low in pure biochemical IRT/PAP protocols. Methods For post hoc analysis the data pool (n=372 906) of a study evaluating a pure biochemical IRT/PAP protocol was used for assessment of the 3-step G-BA protocol in comparison with an alternative screening protocol recommended by the authors...
March 2017: Klinische Pädiatrie
https://www.readbyqxmd.com/read/28444573/the-effect-of-different-types-of-nanoparticles-on-fus-and-tdp-43-solubility-and-subcellular-localization
#16
Jasna Lojk, Sonja Prpar Mihevc, Vladimir Boštjan Bregar, Mojca Pavlin, Boris Rogelj
Increased environmental pollution has been suggested as one of the possible causes for increased incidence of neurodegenerative and developmental disorders. Through the environmental pollution, everyday consumer products and nanomedical applications, we are also exposed to various nanoparticles (NPs). Specific types of NPs have been shown to be able to cause neural damage in vivo through processes such as disruption of the blood-brain barrier, induction of neuroinflammation, increase in oxidative stress and protein aggregation...
April 25, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28444568/effect-of-phosphorus-addition-on-the-reductive-transformation-of-pentachlorophenol-pcp-and-iron-reduction-with-microorganism-involvement
#17
Yongkui Wang, Xianli Liu, Jiexun Huang, Wensheng Xiao, Jiaquan Zhang, Chunqin Yin
The transformation of phosphorus added to the soil environment has been proven to be influenced by the Fe biochemical process, which thereby may affect the transformation of organic chlorinated contaminants. However, the amount of related literatures regarding this topic is limited. This study aimed to determine the effects of phosphorus addition on pentachlorophenol (PCP) anaerobic transformation, iron reduction, and paddy soil microbial community structure. Results showed that the transformation of phosphorus, iron, and PCP were closely related to the microorganisms...
April 25, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28444450/sgnh-hydrolase-type-esterase-domain-containing-cbes-acxe2-a-novel-and-thermostable-acetyl-xylan-esterase-from-caldicellulosiruptor-bescii
#18
Surabhi Soni, Sneha S Sathe, Annamma A Odaneth, Arvind M Lali, Sanjeev K Chandrayan
Caldicellulosiruptor bescii, the most thermophilic cellulolytic bacterium, is rich in hydrolytic and accessory enzymes that can degrade untreated biomass, but the precise role of many these enzymes is unknown. One of such enzymes is a predicted GDSL lipase or esterase encoded by the locus Athe_0553. In this study, this probable esterase named as Cbes-AcXE2 was overexpressed in Escherichia coli. The Ni-NTA affinity purified enzyme exhibited an optimum pH of 7.5 at an optimum temperature of 70 °C. Cbes-AcXE2 hydrolyzed p-nitrophenyl (pNP) acetate, pNP-butyrate, and phenyl acetate with approximately equal efficiency...
April 25, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28444412/biochemical-and-genetic-analyses-of-n-metabolism-in-maize-testcross-seedlings-1-leaves
#19
Ignacio Trucillo Silva, Hari Kishan R Abbaraju, Lynne P Fallis, Hongjun Liu, Michael Lee, Kanwarpal S Dhugga
Aside from the identification of 32 QTL for N metabolism in the seedling leaves of a maize testcross population, alanine aminotransferase was found to be a central enzyme in N assimilation. Excessive application of nitrogen (N) fertilizer to grow commercial crops like maize is a cause of concern because of the runoff of excess N into streams and rivers. Breeding maize with improved N use efficiency (NUE) would reduce environmental pollution as well as input costs for the farmers. An understanding of the genetics underlying N metabolism is key to breeding for NUE...
April 25, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28444337/utility-of-a-repeat-renal-biopsy-in-lupus-nephritis-a-single-centre-experience
#20
Angela Pakozdi, Debasish Pyne, Michael Sheaff, Ravindra Rajakariar
Background.: The role of repeat renal biopsy in lupus nephritis (LN) to guide treatment or predict prognosis has been controversial. We assessed glomerular and tubulointerstitial histological characteristics of serial renal biopsies, correlations with clinical variables and the impact on subsequent management. Methods.: Out of a large single-centre cohort of 270 biopsy- proven LN patients, 66 (24%) had serial biopsies. LN classes based on glomerular pathology were defined according to the International Society of Nephrology/Renal Pathology Society 2003 classification, while tubulointerstitial pathologies were evaluated using the revised Austin's semi-quantitative scoring system...
April 22, 2017: Nephrology, Dialysis, Transplantation
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