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Mirna 143

Nadeeja N Wijayatunga, Mandana Pahlavani, Nishan S Kalupahana, Kameswara Rao Kottapalli, Preethi H Gunaratne, Cristian Coarfa, Latha Ramalingam, Naima Moustaid-Moussa
Obesity contributes to metabolic disorders such as diabetes and cardiovascular disease. Characterization of differences between the main adipose tissue depots, white (WAT) [including subcutaneous (SAT) and visceral adipose tissue (VAT)] and brown adipose tissue (BAT) helps to identify their roles in obesity. Thus, we studied depot-specific differences in whole transcriptome and miRNA profiles of SAT, VAT and BAT from high fat diet (HFD/45% of calories from fat) fed mice using RNA sequencing and small RNA-Seq...
February 6, 2018: Oncotarget
Riikka E Mäkitie, Matthias Hackl, Riitta Niinimäki, Sakari Kakko, Johannes Grillari, Outi Mäkitie
Context: WNT signaling is fundamental to bone health and its aberrant activation leads to skeletal pathologies. A heterozygous missense mutation p.C218G in WNT1, a key WNT pathway ligand, leads to severe early-onset and progressive osteoporosis with multiple peripheral and spinal fractures. Despite the severe skeletal manifestations, conventional bone turnover markers are normal in mutation-positive patients. Objective: This study sought to explore the circulating miRNA pattern in patients with impaired WNT signaling...
March 1, 2018: Journal of Clinical Endocrinology and Metabolism
Yukihiro Akao, Minami Kumasaki, Haruka Shinohara, Nobuhiko Sugito, Yuki Kuranaga, Takuya Tsujino, Yuki Yoshikawa, Yukio Kitade
Despite considerable research on K-Ras inhibitors, none had been developed until now. We synthesized nuclease-resistant synthetic miR-143 (miR-143#12), which strongly silenced K-Ras, its effector signal molecules AKT and ERKs, and the K-Ras activator Sos1. We examined the anti-proliferative effect of miR-143#12 and the mechanism in human colon cancer DLD-1 cell (G13D) and other cell types harboring K-Ras mutations. Cell growth was markedly suppressed in a concentration-dependent manner by miR-143#12 (IC50 : 1...
March 2, 2018: Cancer Science
Swati Agrawal, Thomas Tapmeier, Nilufer Rahmioglu, Shona Kirtley, Krina Zondervan, Christian Becker
BACKGROUND: Endometriosis is a common disorder of the reproductive age group, characterised by the presence of ectopic endometrial tissue. The disease not only causes enormous suffering to the affected women, but also brings a tremendous medical and economic burden to bear on society. There is a long lag phase between the onset and diagnosis of the disease, mainly due to its non-specific symptoms and the lack of a non-invasive test. Endometriosis can only be diagnosed invasively by laparoscopy...
February 17, 2018: International Journal of Molecular Sciences
Vitalie Samoil, Maude Dagenais, Vinupriya Ganapathy, Jerry Aldridge, Anastasia Glebov, Armando Jardim, Paula Ribeiro
Exosomes are small vesicles of endocytic origin, which are released into the extracellular environment and mediate a variety of physiological and pathological conditions. Here we show that Schistosoma mansoni releases exosome-like vesicles in vitro. Vesicles were purified from culture medium by sucrose gradient fractionation and fractions containing vesicles verified by western blot analyses and electron microscopy. Proteomic analyses of exosomal contents unveiled 130 schistosome proteins. Among these proteins are common exosomal markers such as heat shock proteins, energy-generating enzymes, cytoskeletal proteins, and others...
February 19, 2018: Scientific Reports
Yun-Peng Jin, Yun-Ping Hu, Xiang-Song Wu, Yao-Shi Wu, Yuan-Yuan Ye, Huai-Feng Li, Yong-Chen Liu, Lin Jiang, Fa-Tao Liu, Yi-Jian Zhang, Ya-Juan Hao, Xi-Yong Liu, Ying-Bin Liu
Gallbladder cancer (GBC) is the most common malignant tumour of the biliary track system. Angiogenesis plays a pivotal role in the development and progression of malignant tumours. miR-143-3p acts as a tumour suppressor in various cancers. Their role in GBC is however less well defined. Here we show that the expression levels of miR-143-3p were decreased in human GBC tissues compared with the non-tumour adjacent tissue (NAT) counterparts and were closely associated with overall survival. We discovered that miR-143-3p was a novel inhibitor of tumour growth and angiogenesis in vivo and in vitro...
February 7, 2018: Cell Death & Disease
Gabriella B Oliveira, Luciana C A Regitano, Aline S M Cesar, James M Reecy, Karina Y Degaki, Mirele D Poleti, Andrezza M Felício, James E Koltes, Luiz L Coutinho
BACKGROUND: The amount of intramuscular fat can influence the sensory characteristics and nutritional value of beef, thus the selection of animals with adequate fat deposition is important to the consumer. There is growing knowledge about the genes and pathways that control the biological processes involved in fat deposition in muscle. MicroRNAs (miRNAs) belong to a well-conserved class of non-coding small RNAs that modulate gene expression across a range of biological functions in animal development and physiology...
February 7, 2018: BMC Genomics
Juber Herrera-Uribe, Sara Zaldívar-López, Carmen Aguilar, Cristina Luque, Rocío Bautista, Ana Carvajal, M Gonzalo Claros, Juan J Garrido
Salmonellosis is a gastrointestinal disease caused by non-typhoidal Salmonella serovars such as Salmonella Typhimurium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast reservoir for disease. After intestinal colonization, Salmonella Typhimurium reaches mesenteric lymph nodes (MLN), where infection is controlled avoiding systemic spread. Although the molecular basis of this infection has been extensively studied, little is known about how microRNA (miRNA) regulate the expression of proteins involved in the Salmonella-host interaction...
February 1, 2018: Veterinary Research
Qi Li, Xiaohong Peng, Haoran Huang, Jingjing Li, Fan Wang, Jie Wang
This study aimed to identify miRNAs that may contribute to the pathogenesis of sudden sensorineural hearing loss (SSNHL) by RNA-seq (RNA-sequencing).RNA was extracted from SSNHL patients and healthy volunteers, respectively. Sequencing was performed on HiSeq4000 platform. After filtering, clean reads were mapped to the human reference genome hg19. Differential expression analysis of miRNAs between the SSNHL samples and the normal samples was performed using DEseq to identify differentially expressed microRNAs (DEMs)...
November 2017: Medicine (Baltimore)
Iman Jahanbani, Abeer Al-Abdallah, Rola Ali, Nabeel Al-Brahim, Olusegun Mojiminiyi
BACKGROUND: Papillary thyroid carcinoma (PTC) variants have several overlapping clinical and pathological features. The World Health Organization (WHO) published recently a new classification of thyroid tumors containing significant revisions. the encapsulated papillary thyroid carcinoma (EPTC) has been recognized as a distinctive variant of PTC. The noninvasive encapsulated follicular variant of PTC has been reclassified as "Noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP)"...
January 29, 2018: Thyroid: Official Journal of the American Thyroid Association
Sofia E Gomes, Diane M Pereira, Catarina Roma-Rodrigues, Alexandra R Fernandes, Pedro M Borralho, Cecília M P Rodrigues
MicroRNAs (miRNAs) regulate a wide variety of biological processes, including tumourigenesis. Altered miRNA expression is associated with deregulation of signalling pathways, which in turn cause abnormal cell growth and de-differentiation, contributing to cancer. miR-143 and miR-145 are anti-tumourigenic and influence the sensitivity of tumour cells to chemotherapy and targeted therapy. Comparative proteomic analysis was performed in HCT116 human colon cancer cells stably transduced with miR-143 or miR-145...
2018: PloS One
Nan Zhang, Zhongyi Li, Fuding Bai, Na Ji, Yichun Zheng, Yi Li, Jimin Chen, Xiawa Mao
Although alterations in microRNA (miRNA) expression have been previously investigated prostate cancer, the expression of miRNAs specifically in benign prostate hyperplasia (BPH) of the prostatic stroma remains to be fully elucidated. In the present study, miRNAs and gene expression profiles were investigated using microarray analysis and reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) in BPH tissue to clarify the associations between miRNA expression and target genes. Prostate tissue samples from five patients with BPH and five healthy men were analyzed using human Affymetrix miRNA and mRNA microarrays and differentially expressed miRNAs were validated using RT‑qPCR with 30 BPH and 5 healthy control samples...
December 19, 2017: Molecular Medicine Reports
Lihong Ruan, Yuanyuan Xie, Fangmei Liu, Xuehua Chen
OBJECTIVE: This study aims to identify serum microRNAs (miRNAs) related to ovarian cancer. STUDY DESIGN: MiRNA profiling data (GSE79943) were generated from the Gene Expression Omnibus, including 3 serum samples from healthy individuals and 4/3/16/6 serum samples from patients with ovarian cancer stage I/II/III/IV. Differentially expressed miRNAs (DEmiRNAs) were identified between controls and ovarian cancer stage I/II/III/IV by using limma package (p-value <0...
January 9, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
Jessica E Frith, Gina D Kusuma, James Carthew, Fanyi Li, Nicole Cloonan, Guillermo A Gomez, Justin J Cooper-White
Mechanotransduction is a strong driver of mesenchymal stem cell (MSC) fate. In vitro, variations in matrix mechanics invoke changes in MSC proliferation, migration and differentiation. However, when incorporating MSCs within injectable, inherently soft hydrogels, this dominance over MSC response substantially limits our ability to couple the ease of application of hydrogels with efficiently directed MSC differentiation, especially in the case of bone generation. Here, we identify differential miRNA expression in response to varying hydrogel stiffness and RhoA activity...
January 17, 2018: Nature Communications
Yang Yuan, Li Jiaoming, Wang Xiang, Liu Yanhui, Jiang Shu, Gou Maling, Mao Qing
Cross-talk between competitive endogenous RNAs (ceRNAs) may play a critical role in revealing potential mechanisms of tumor development and physiology. Glioblastoma is the most common type of malignant primary brain tumor, and the mechanisms of tumor genesis and development in glioblastoma are unclear. Here, to investigate the role of non-coding RNAs and the ceRNA network in glioblastoma, we performed paired-end RNA sequencing and microarray analyses to obtain the expression profiles of mRNAs, lncRNAs, circRNAs and miRNAs...
January 15, 2018: Journal of Neuro-oncology
Meng Jiao, Hong-Zhao You, Xin-Ying Yang, Hui Yuan, Yu-Lin Li, Wen-Xian Liu, Mei Jin, Jie Du
Circulating miRNAs are proposed as a biomarker of heart disease. This study evaluated whether circulating miRNAs could be used as a biomarker for childhood dilated cardiomyopathy (CDCM). A total of 28 participants were enrolled in a discovery set, including patients with CDCM (n = 16) and healthy children (n = 12). The cardiac function of patients with CDCM was characterized by echocardiography and serum miRNA profiles of all participants were assessed by miRNA sequencing. After miRNA profiling, we quantitatively confirmed 148 regulated miRNAs in patients with CDCM compared with healthy subjects, and none were downregulated...
January 15, 2018: Scientific Reports
Xiangran Sun, Guo Dai, Ling Yu, Qingzhu Hu, Jingteng Chen, Weichun Guo
Osteosarcoma (OS) is the most common type of primary malignant bone tumor and mainly occurs in children and adolescent. Because of its early migration and invasion, OS has a poor prognosis. It has been reported that mircoRNAs (miRNAs) play a crucial role in the occurrence and development of multiple tumors. In this study, we identified the aberrant-expression of miR-143-3p in osteosarcoma and examined the role of miR-143-3p in OS development. Further, we searched the miR-143-3p target gene and verified its accuracy by luciferase experiments...
January 12, 2018: Scientific Reports
J Laco, H Kovarikova, M Chmelarova, H Vosmikova, K Sieglova, I Bubancova, P Dundr, K Nemejcova, J Michalek, P Celakovsky, R Mottl, I Sirak, M Vosmik, I Marek, T Geryk, J Mejzlik, J Satankova, A Ryska
The aim of this study was a detailed clinicopathological investigation of sinonasal NUT midline carcinoma (NMC), including analysis of DNA methylation and microRNA (miRNA) expression. Three (5%) cases of NMC were detected among 56 sinonasal carcinomas using immunohistochemical screening and confirmed by fluorescence in situ hybridization. The series comprised 2 males and 1 female, aged 46, 60, and 65 years. Two tumors arose in the nasal cavity and one in the maxillary sinus. The neoplasms were staged pT1, pT3, and pT4a (all cN0M0)...
2018: Neoplasma
Da-Cen Lin, Jia-Bing Lin, Zhou Chen, Rong Chen, Chun-Yu Wan, Shao-Wei Lin, Qi-Shuang Ruan, Huang-Yuan Li, Si-Ying Wu
Objective: To evaluate the effects of environmental factors and microRNAs (miRNAs) (miR-126, miR-143, and miR-145) on the risk of coronary heart disease (CHD). Methods: A frequency-matched case-control study (450 patients, 450 controls) was conducted from April 2014 to December 2016 in Fuzhou City, China. Environmental factors were investigated using a self-administered questionnaire, and the expression levels of miR-126, miR-143, and miR-145 were determined by quantitative real-time Polymerase Chain Reaction (PCR) in peripheral blood mononuclear cells...
November 2017: Journal of Geriatric Cardiology: JGC
Mao Guoping, Liu Ran, Qin Yanru
Recent studies have suggested that the dysregulation of microRNAs (miRNAs) plays a critical role in the progression ofhuman cancers including gastric cancer (GC). miR-143 had been reported to function as tumor suppressor in GC. However, the exact molecular mechanism of how miR-143 participated in GC progression remains to be well studied. In this present study, we revealed that the expression of miR-143 was significantly downregulated in human GC tissues and cell lines compared with normal tissues and normal gastric epithelium cell line...
January 10, 2018: Oncology Research
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