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https://www.readbyqxmd.com/read/29786845/a-simple-and-portable-screening-method-for-adulterated-olive-oils-using-the-hand-held-ftir-spectrometer-and-chemometrics-tools
#1
Meng Pan, Suqin Sun, Qun Zhou, Jianbo Chen
Extra virgin olive oil has been one of the most common targets for economically motivated food adulteration. This research employed the hand-held FTIR spectrometer to develop the simple and portable screening methods for extra virgin olive oils adulterated with other cheaper vegetable oils. With the help of the ATR probe and chemometrics tools, oil samples can be measured directly without any pretreatment, then the spectra can be interpreted automatically to determine the possibility of adulteration and estimate the content of adulterant oil...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29784322/peanut-flour-aggregation-with-polyphenolic-extracts-derived-from-peanut-skin-inhibits-ige-binding-capacity-and-attenuates-rbl-2h3-cells-degranulation-via-mapk-signaling-pathway
#2
Rishipal R Bansode, Nathalie J Plundrich, Priscilla D Randolph, Mary Ann Lila, Leonard L Williams
This study investigates the anti-allergic properties of peanut skin polyphenols (PSP)-enriched peanut (PN) protein aggregates. PSP was blended with PN flour at concentrations of 5, 10, 15, 30, and 40% (w/w). Rat basophil leukemia cells (RBL-2H3) were sensitized with either anti-DNP-IgE or PN-allergic plasma followed by co-exposure to unmodified PN flour (control) or PSP-PN protein aggregates and Ca2+ ionophore, ionomycin. Immunoblotting and staining were performed to measure the IgE binding capacity of PSP-PN aggregates...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29779209/gata3-hypermethylation-and-foxp3-hypomethylation-are-associated-with-sustained-protection-and-bystander-effect-following-epicutaneous-immunotherapy-in-peanut-sensitized-mice
#3
Lucie Mondoulet, Vincent Dioszeghy, Florence Busato, Camille Plaquet, Véronique Dhelft, Kevin Bethune, Laurence Leclere, Christian Daviaud, Mélanie Ligouis, Hugh Sampson, Christophe Dupont, Jörg Tost
BACKGROUND: Epicutaneous immunotherapy (EPIT) is a promising method for treating food allergies. In animal models, EPIT induces sustained unresponsiveness and prevents further sensitization mediated by Tregs. Here, we elucidate the mechanisms underlying the therapeutic effect of EPIT, by characterizing the kinetics of DNA methylation changes in sorted cells from spleen and blood and by evaluating its persistence and bystander effect compared to oral immunotherapy (OIT). METHODS: BALB/c mice orally sensitized to peanut proteins (PPE) were treated by EPIT using a PPE-patch or by PPE-OIT...
May 19, 2018: Allergy
https://www.readbyqxmd.com/read/29774047/genome-sequencing-and-analysis-of-the-peanut-b-genome-progenitor-arachis-ipaensis
#4
Qing Lu, Haifen Li, Yanbin Hong, Guoqiang Zhang, Shijie Wen, Xingyu Li, Guiyuan Zhou, Shaoxiong Li, Hao Liu, Haiyan Liu, Zhongjian Liu, Rajeev K Varshney, Xiaoping Chen, Xuanqiang Liang
Peanut ( Arachis hypogaea L.), an important leguminous crop, is widely cultivated in tropical and subtropical regions. Peanut is an allotetraploid, having A and B subgenomes that maybe have originated in its diploid progenitors Arachis duranensis (A-genome) and Arachis ipaensis (B-genome), respectively. We previously sequenced the former and here present the draft genome of the latter, expanding our knowledge of the unique biology of Arachis . The assembled genome of A. ipaensis is ~1.39 Gb with 39,704 predicted protein-encoding genes...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29772091/sensitisation-to-outdoor-and-indoor-fungi-in-atopic-dermatitis-patients-and-the-relation-to-the-occurrence-of-food-allergy-to-peanuts-and-walnuts
#5
J Čelakovská, J Bukač, K Ettler, J Vaneckova, K Ettlerova, J Krejsek
The aim of this study is the evaluation of the relation between the sensitisation to outdoor and indoor fungi and allergy to peanuts and walnuts in atopic dermatitis patients aged 14 years and older. The complete dermatological and allergological examinations were perfomed in all included patients; the occurrence of food allergy to peanuts and walnuts was recorded (specific IgE, skin prick test, history of allergic reaction) and the sensitisation to mixture of outdoor fungi and indoor fungi was examined (skin prick test, specific IgE)...
May 17, 2018: Mycoses
https://www.readbyqxmd.com/read/29759900/detection-of-the-type-iii-secretion-system-and-its-phylogenetic-and-symbiotic-characterization-in-peanut-bradyrhizobia-isolated-from-guangdong-province-china
#6
Huaqin Ruan, Meijuan Hu, Jingyu Chen, Xue Li, Ting Li, Yongxiu Lai, En Tao Wang, Jun Gu
The distribution of rhcRST and rhcJ-C1 fragments located in different loci of the type III secretion system (T3SS) gene cluster in the peanut-nodulating bradyrhizobia isolated from Guangdong Province, China was investigated by PCR-based sequencing. T3SS was detected in approximately one-third of the peanut bradyrhizobial strains and the T3SS-harboring strains belonging to different Bradyrhizobium genomic species. Diverse T3SS groups corresponding to different symbiotic gene types were defined among the 23 T3SS-harboring strains...
April 8, 2018: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29759105/the-glycaemic-index-and-insulinaemic-index-of-commercially-available-breakfast-and-snack-foods-in-an-asian-population
#7
Wei Shuan Kimberly Tan, Wei Jie Kevin Tan, Shalini D/O Ponnalagu, Katie Koecher, Ravi Menon, Sze-Yen Tan, Christiani J Henry
A low-glycaemic-index (GI) breakfast has been shown to lower blood glucose levels throughout the day. A wide variety of breakfast foods are consumed, but their GI values are largely unknown, hence limiting consumers' ability to select healthier options. This study investigated the GI values of ten common breakfast (five Asian and five Western) foods in this region using a randomised, cross-over study design. Participants arrived after an overnight fast, and fasting blood sample was taken before participants consumed test foods...
May 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29755500/haplotype-based-genotyping-in-polyploids
#8
Josh P Clevenger, Walid Korani, Peggy Ozias-Akins, Scott Jackson
Accurate identification of polymorphisms from sequence data is crucial to unlocking the potential of high throughput sequencing for genomics. Single nucleotide polymorphisms (SNPs) are difficult to accurately identify in polyploid crops due to the duplicative nature of polyploid genomes leading to low confidence in the true alignment of short reads. Implementing a haplotype-based method in contrasting subgenome-specific sequences leads to higher accuracy of SNP identification in polyploids. To test this method, a large-scale 48K SNP array (Axiom Arachis2) was developed for Arachis hypogaea (peanut), an allotetraploid, in which 1,674 haplotype-based SNPs were included...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29754191/the-unique-molecular-signatures-of-contact-dermatitis-and-implications-for-treatment
#9
REVIEW
Alexandra Leonard, Emma Guttman-Yassky
Irritant contact dermatitis (ICD) and allergic contact dermatitis (ACD) are common skin disorders that are characterized by inflammation, oozing, crusting, and pruritus. Atopic dermatitis (AD) is an inflammatory skin disease characterized by immune and barrier abnormalities and is additionally a risk factor for acquiring ICD and ACD. New work on allergic sensitization to common allergens (e.g., nickel and fragrance) in human skin has shown that different allergens have distinct molecular fingerprinting. For example, nickel promotes strong Th1/Th17 polarization, whereas fragrance allergy causes Th2/Th22 skewing, which is similar to the phenotype of AD...
May 12, 2018: Clinical Reviews in Allergy & Immunology
https://www.readbyqxmd.com/read/29751673/expression-of-ahdreb1-an-ap2-erf-transcription-factor-gene-from-peanut-is-affected-by-histone-acetylation-and-increases-abscisic-acid-sensitivity-and-tolerance-to-osmotic-stress-in-arabidopsis
#10
Baihong Zhang, Liangchen Su, Bo Hu, Ling Li
Drought stress negatively affects plant growth and development. An increasing number of reports have revealed the involvement of APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors (TFs) in biotic and abiotic stress regulation in plants. However, research on these TFs in the peanut plant (Arachis hypogaea) has been limited. Here, we isolated a full-length coding sequence (CDS) of the AP2/ERF family gene AhDREB1 from the peanut plant and showed that its expression was induced by Polyethylene Glycol (PEG) 6000 and exogenous abscisic acid (ABA) treatment...
May 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29751668/electrochemical-immunosensor-for-detection-of-aflatoxin-b%C3%A2-based-on-indirect-competitive-elisa
#11
Farah Asilah Azri, Rashidah Sukor, Jinap Selamat, Fatimah Abu Bakar, Nor Azah Yusof, Reza Hajian
Mycotoxins are the secondary toxic metabolites produced naturally by fungi. Analysis of mycotoxins is essential to minimize the consumption of contaminated food and feed. In this present work, an ultrasensitive electrochemical immunosensor for the detection of aflatoxin B₁ (AFB₁) was successfully developed based on an indirect competitive enzyme-linked immunosorbent assay (ELISA). Various parameters of ELISA, including antigen⁻antibody concentration, blocking agents, incubation time, temperature and pH of reagents, were first optimized in a 96-well microtiter plate to study the antigen⁻antibody interaction and optimize the optimum parameters of the assay...
May 11, 2018: Toxins
https://www.readbyqxmd.com/read/29751153/the-health-and-economic-outcomes-of-peanut-allergy-management-practices
#12
Marcus Shaker, Matthew Greenhawt
BACKGROUND: Peanut allergy is managed with strict avoidance, epinephrine carriage, and promptly treating reactions. OBJECTIVES: Assess the health and economic benefits of pre-emptively injecting epinephrine for peanut ingestion in the absence of any symptoms, and avoiding products with peanut precautionary allergen labeling (PAL). METHODS: We used Markov modeling and simulations, assuming a base-case 10-fold fatality risk increase for less conservative management, with sensitivity analysis investigating 100-1,000-fold increased fatality risk, incorporating risks of accidental exposures, reactions, fatality, and family costs of food allergy...
May 8, 2018: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/29744525/mapping-of-a-dominant-rust-resistance-gene-revealed-two-r-genes-around-the-major-rust_qtl-in-cultivated-peanut-arachis-hypogaea-l
#13
Suvendu Mondal, Anand M Badigannavar
A consensus rust QTL was identified within a 1.25 cM map interval of A03 chromosome in cultivated peanut. This map interval contains a TIR-NB-LRR R gene and four pathogenesis-related genes. Disease resistance in plants is manifested due to the specific interaction between the R gene product and its cognate avirulence gene product (AVR) in the pathogen. Puccinia arachidis Speg. causes rust disease and inflicts economic damages to peanut. Till now, no experimental evidence is known for the action of R gene in peanut for rust resistance...
May 9, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29743467/-inhibition-of-growth-of-seed-borne-fungi-and-aflatoxin-production-on-stored-peanuts-by-allyl-isothiocyanate-vapor
#14
Kiyoshi Okano, Chikako Nishioka, Tetsuya Iida, Yuzi Ozu, Misao Kaneko, Yuko Watanabe, Yuichi Mizukami, Masakatsu Ichinoe
Aspergillus parasiticus contamination of peanuts results in the production of highly toxic metabolites, such as aflatoxin B1 , B2 , G1 and G2 , and its incidence in imported peanuts is reported to be increasing. Here, we examined whether the antifungal compound allyl isothiocyanate (AIT), which is present in mustard seed, could inhibit the growth of seed-borne fungi and aflatoxin-producing fungi. Peanuts produced in China and Japan were inoculated with A. parasiticus and exposed to AIT vapor released by a commercial mustard seed extract in closed containers under controlled conditions of temperature and humidity...
2018: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/29743132/comparison-of-real-time-polymerase-chain-reaction-and-enzyme-linked-immunosorbent-assay-for-sensitive-and-quantitative-detection-of-hazelnuts-in-nut-pastes
#15
Ľubica Piknová, Veronika Janská, Tomáš Kuchta, Peter Siekel
Background : Hazelnuts, being a frequent agent of allergenic reactions, need to be detected in food products. Thus, it is necessary to develop and further investigate appropriate methods for detection. Objective : The aim of the study was to compare the analysis of nut pastes (peanut paste spiked with different amounts of hazelnut paste) as a model of contamination of confectionery. Methods : Real-time PCR and sandwich ELISA (RidaScreen Hazelnut Fast Kit) were used. Results : For real-time PCR, LOQ of 2 mg/kg and a quantification range from 2 to 10 000 mg/kg were determined...
May 9, 2018: Journal of AOAC International
https://www.readbyqxmd.com/read/29739687/the-role-of-regulatory-t-cells-in-the-acquisition-of-tolerance-to-food-allergens-in-children
#16
REVIEW
E Bernaldo de Quiros, E Seoane-Reula, E Alonso-Lebrero, M Pion, R Correa-Rocha
Food allergy is a pathological immune reaction that identifies certain harmless food proteins, usually tolerated by the majority of the people, as a threat. The prevalence of these food allergies is increasing worldwide and currently affects 8% of children. Exacerbated reactions to milk, egg and peanut are the most frequent in the pediatric population. It is well known that allergic diseases are a type 2 T-helper (Th2) immune response, characterized by the elevated production of IgE antibodies. However, little is known about the immune mechanisms responsible for the development of clinical tolerance toward food allergens...
May 5, 2018: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/29735106/chitosan-active-films-containing-agro-industrial-residue-extracts-for-shelf-life-extension-of-chicken-restructured-product
#17
Juan S Serrano-León, Keityane B Bergamaschi, Cristiana M P Yoshida, Erick Saldaña, Miriam M Selani, Juan D Rios-Mera, Severino M Alencar, Carmen J Contreras-Castillo
This study aimed to develop chitosan films incorporating natural antioxidants from peanut skin (EPS) and pink pepper residue (EPP) extracts, as well as to evaluate their effects on lipid oxidation, pH, color, and microbial counts of a restructured chicken product. EPS had higher phenolic content and antioxidant activity compared to EPP. When both extracts were applied to chicken meat and the chitosan films, there were no differences for color, pH and total mesophilic counts compared to control at the end of the storage period...
June 2018: Food Research International
https://www.readbyqxmd.com/read/29733931/a-novel-step-towards-immobilization-of-biocatalyst-using-agro-waste-and-its-application-for-ester-synthesis
#18
Prerana D Tomke, Virendra K Rathod
This work explains the utilization of agro-waste (coconut and peanut shell) to produce mesoporous activated carbon which further utilized as a support material for lipase immobilization (Candida antarctica B, CALB). Various parameters affecting the binding of enzyme to activated carbon with high surface area (1603 m2  g-1 ) were optimized. Maximum 200 μg g-1 CALB has been loaded at 40 °C and pH 6.8 in 12 h by using glutaraldehyde as a cross-linker. The operational parameters such as pH (5.8-8...
May 4, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29732294/shellfish-crustacean-oral-allergy-syndrome-among-national-service-pre-enlistees-in-singapore
#19
Bernard Yu-Hor Thong, Shalini Arulanandam, Sze-Chin Tan, Teck-Choon Tan, Grace Yin-Lai Chan, Justina Wei-Lyn Tan, Mark Chong-Wei Yeow, Chwee-Ying Tang, Jinfeng Hou, Khai-Pang Leong
Background: All Singaporean males undergo medical screening prior to compulsory military service. A history of possible food allergy may require referral to a specialist Allergy clinic to ensure that special dietary needs can be taken into account during field training and deployment. Objective: To study the pattern of food allergy among pre-enlistees who were referred to a specialist allergy clinic to work up suspected food allergy. Methods: Retrospective study of all pre-enlistees registered in the Clinical Immunology/Allergy New Case Registry referred to the Allergy Clinic from 1 August 2015 to 31 May 2016 for suspected food allergy...
April 2018: Asia Pacific Allergy
https://www.readbyqxmd.com/read/29731128/a-novel-human-mast-cell-activation-test-for-peanut-allergy
#20
Alexandra F Santos, Natália Couto-Francico, Natalia Bécares, Matthew Kwok, Henry T Bahnson, Gideon Lack
No abstract text is available yet for this article.
April 26, 2018: Journal of Allergy and Clinical Immunology
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