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https://www.readbyqxmd.com/read/28433750/nanoemulsion-based-electrolyte-triggered-in-situ-gel-for-ocular-delivery-of-acetazolamide
#1
Nadia Morsi, Magdy Ibrahim, Hanan Refai, Heba El Sorogy
In the present work the antiglaucoma drug, acetazolamide, was formulated as an ion induced nanoemulsion-based in situ gel for ocular delivery aiming a sustained drug release and an improved therapeutic efficacy. Different acetazolamide loaded nanoemulsion formulations were prepared using peanut oil, tween 80 and/or cremophor EL as surfactant in addition to transcutol P or propylene glycol as cosurfactant. Based on physicochemical characterization, the nanoemulsion formulation containing mixed surfactants and transcutol P was selected to be incorporated into ion induced in situ gelling systems composed of gellan gum alone and in combination with xanthan gum, HPMC or carbopol...
April 19, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28433057/low-cost-humic-acid-bonded-silica-as-an-effective-solid-phase-extraction-sorbent-for-convenient-determination-of-aflatoxins-in-edible-oils
#2
Neng-Zhi Zhou, Ping Liu, Xiao-Chuan Su, Yan-Hua Liao, Ning-Sheng Lei, Yong-Hong Liang, Shao-Huan Zhou, Wen-Si Lin, Jie Chen, Yu-Qi Feng, Yang Tang
Aflatoxins (AFs) are highly toxic, mutagenic, carcinogenic, and teratogenic secondary metabolites produced by the toxigenic fungi Aspergillus flavus and Aspergillus parasiticus. AFs tend to contaminate a wide range of foods which is a serious and recurring food safety problem worldwide. Currently, immunoaffinity chromatography (IAC) has become the most conventional sample clean-up method for determining AFs in foodstuffs. However, IAC method is limited in the large-scale food analysis because it requires the use of expensive disposable cartridges and the IA procedure is time-consuming...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28431184/congener-specific-disposition-of-chiral-polychlorinated-biphenyls-in-lactating-mice-and-their-offspring-implications-for-pcb-developmental-neurotoxicity
#3
Izabela Kania-Korwel, Tracy Lukasiewicz, Christopher D Barnhart, Marianna Stamou, Haeun Chung, Kevin M Kelly, Stelvio Bandiera, Pamela J Lein, Hans-Joachim Lehmler
Chiral polychlorinated biphenyl (PCB) congeners have been implicated by laboratory and epidemiological studies in PCB developmental neurotoxicity. These congeners are metabolized by cytochrome P450 (P450) enzymes to potentially neurotoxic hydroxylated metabolites (OH-PCBs). The present study explores the enantioselective disposition and toxicity of two environmentally relevant, neurotoxic PCB congeners and their OH-PCB metabolites in lactating mice and their offspring following dietary exposure of the dam. Female C57BL/6N mice (8 weeks old) were fed daily, beginning two weeks prior to conception and continuing throughout gestation and lactation, with 3...
April 14, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28430142/initial-spore-density-has-an-influence-on-ochratoxin-a-content-in-aspergillus-ochraceus-cgmcc-3-4412-in-pdb-and-its-interaction-with-seeds
#4
Caiyan Li, Yanmin Song, Lu Xiong, Kunlun Huang, Zhihong Liang
The morphology and secondary metabolism of Aspergillus spp. are associated with initial spore density (ISD). Fatty acids (FA) are involved in the biosynthesis of aflatoxins (AF) through Aspergillus quorum sensing (QS). Here, we studied how ochratoxin A (OTA) was regulated by spore density in Aspergillus ochraceus CGMCC 3.4412. The results contribute to understanding the role of spore density in morphogenesis, OTA biosynthesis, and host-pathogen interactions. When A. ochraceus was grown in Potato Dextrose Broth (PDB) media at different spore densities (from 10¹ to 10⁶ spores/mL), more OTA was produced when ISD were increased, but a higher level of ISD inhibited OTA biosynthesis...
April 21, 2017: Toxins
https://www.readbyqxmd.com/read/28427612/study-of-ultrasonic-cavitation-during-extraction-of-the-peanut-oil-at-varying-frequencies
#5
Lei Zhang, Cunshan Zhou, Bei Wang, Abu El-Gasim A Yagoub, Haile Ma, Xiao Zhang, Mian Wu
The ultrasonic extraction of oils is a typical physical processing technology. The extraction process was monitored from the standpoint of the oil quality and efficiency of oil extraction. In this study, the ultrasonic cavitation fields were measured by polyvinylidene fluoride (PVDF) sensor. Waveform of ultrasonic cavitation fields was gained and analyzed. The extraction yield and oxidation properties were compared. The relationship between the fields and cavitation oxidation was established. Numerical calculation of oscillation cycle was done for the cavitation bubbles...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28426195/one-step-fabrication-of-non-fluorinated-transparent-super-repellent-surfaces-with-tunable-wettability-functioning-both-in-air-and-oil
#6
Mengying Long, Shan Peng, Xiaojun Yang, Wanshun Deng, Ni Wen, Kai Miao, Gongyun Chen, Xinrui Miao, Wenli Deng
In this paper, we have developed a one-step thermal treatment of polydimethylsiloxane (PDMS) liquid to create transparent super-repellent surface (TSS) and super-repellent powder. They are super-repellent toward water and ethylene glycol. During the one-step thermal treatment, PDMS soot generated and deposited onto glass slide (GS) surface to fabricate the TSS without fluorosilane modification. The facilely obtained TSS presented superhydrophobicity and self-cleaning property when immersed into very low-surface-tension oils such as petroleum ether, hexadecane, peanut oil, and dodecane...
April 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28425668/pectin-hemicellulose-or-lignin-impact-of-the-biowaste-source-on-the-performance-of-hard-carbons-for-sodium-ion-batteries
#7
Xinwei Dou, Ivana Hasa, Maral Hekmatfar, Thomas Diemant, R Juergen Behm, Daniel Buchholz, Stefano Passerini
Hard carbons are currently the most widely used negative electrode materials in Na-ion batteries. This is due to their promising electrochemical performance with capacities of 200-300 mAh g-1 and stable long-term cycling. However, an abundant and cheap carbon source is necessary in order to comply with the low-cost philosophy of the Na-ion technology. As a matter of fact, many biologic or waste materials have been used to synthesize hard carbons but the impact of the precursors on the final properties of the anode material is not fully understood...
April 20, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28425254/wheezing-asthma-and-atopy-in-premature-infants-at-2-years-of-age
#8
Sevim Ünal, Ayşenur Kaya, Leyla Bilgin, Emine Misirlioğlu, Can Naci Kocabaş
BACKGROUND/AIM: We aimed to evaluate wheezing, bronchial asthma (BA), and atopy in premature infants at 2 years of age via a cross-sectional study. MATERIALS AND METHODS: Premature infants at <37 weeks of gestational age (GA) were assessed for atopy by skin-prick test and serum immunoglobulin E level at 2 years of age. The family's and infant's histories of allergy, BA, atopy, and wheezing were obtained by questionnaire and from hospital records. RESULTS: There were 98 infants, with mean birth weight (BW) 1517...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28424944/indirect-interactions-between-arbuscular-mycorrhizal-fungi-and-spodoptera-exigua-alter-photosynthesis-and-plant-endogenous-hormones
#9
Lei He, Changyou Li, Runjin Liu
Peanut (Arachis hypogaea Linn. cv: Luhua 11) and tomato (Lycopersicon esculentum Mill. cv: Zhongshu 4) were inoculated with arbuscular mycorrhizal fungi (AMF) Funneliformis mosseae BEG167 (Fm), Rhizophagus intraradices BEG141 (Ri), and Glomus versiforme Berch (Gv), and/or Spodoptera exigua (S. exigua) under greenhouse conditions. Results indicated that feeding by S. exigua had little influence on colonization of peanut plants by AMF, but improved colonization of tomato by Fm and Gv. Feeding by S. exigua had little influence on leaf net photosynthetic rate, transpiration rate, and stomatal conductance of nonmycorrhizal peanut plants but significantly improved net photosynthetic rate and transpiration rate of mycorrhizal plants of both hosts...
April 20, 2017: Mycorrhiza
https://www.readbyqxmd.com/read/28424511/response-of-peanut-arachis-hypogaea-roots-to-the-presence-of-beneficial-and-pathogenic-fungi-by-transcriptome-analysis
#10
Kun Hao, Feng Wang, Xiangqun Nong, Mark Richard McNeill, Shaofang Liu, Guangjun Wang, Guangchun Cao, Zehua Zhang
Entomopathogenic fungus Metarhizium anisopliae obtain survival benefit meanwhile promote the nutrient absorption of root as an endophyte. However, little is known concerning molecular mechanisms in the process. We performed the transcriptome sequencing of A. hypogaea roots inoculated M. anisopliae and pathogenic Fusarium axysporum, respectively. There were 81323 unigenes from 132023 transcripts. Total 203 differentially expressed genes (DEGs) respond to the two fungi, including specific 76 and 34 DEGs distributed respectively in M...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28423454/uk-neqas-survey-of-allergen-component-testing-across-the-united-kingdom-and-other-european-countries
#11
R Saleem, C Keymer, D Patel, W Egner, A W Rowbottom
The clinical utility of molecular diagnostic approaches in allergy investigation is being recognized increasingly to play a significant role in the management of allergic patients. Determining the sensitization pattern, which is best achieved through the use of component resolved diagnostics (CRD), allows effective risk stratification, appropriate treatment and patient selection for immunotherapy. In order to assess the diagnostic service provisions for in-vitro allergy testing across Europe, a survey was carried out via the total immunoglobulin (Ig)E and specific IgE external quality assurance schemes run by UK National External Quality Assessment Service (NEQAS) Immunology, Immunochemistry and Allergy...
April 19, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28423007/recombinants-from-the-crosses-between-amphidiploid-and-cultivated-peanut-arachis-hypogaea-for-pest-resistance-breeding-programs
#12
Ailton Ferreira de Paula, Naiana Barbosa Dinato, Bianca Baccili Zanotto Vigna, Alessandra Pereira Fávero
Peanut is a major oilseed crop worldwide. In the Brazilian peanut production, silvering thrips and red necked peanut worm are the most threatening pests. Resistant varieties are considered an alternative to pest control. Many wild diploid Arachis species have shown resistance to these pests, and these can be used in peanut breeding by obtaining hybrid of A and B genomes and subsequent polyploidization with colchicine, resulting in an AABB amphidiploid. This amphidiploid can be crossed with cultivated peanut (AABB) to provide genes of interest to the cultivar...
2017: PloS One
https://www.readbyqxmd.com/read/28421513/primary-prevention-of-food-allergy
#13
REVIEW
Matthew J Greenhawt, David M Fleischer
Food allergy is estimated to affect approximately 8% of children in the USA. This is a disease without any known treatment or cure and, for some, a disease that can be quite severe, even life-threatening. While recent advances in potential treatment have made remarkable strides, with two food-targeted immunotherapy products now in phase III trials, perhaps the biggest gains in the field have come in the advent of potential preventative strategies to avoid the development of food allergy in high-risk individuals...
April 2017: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/28421098/fine-mapping-the-branching-habit-trait-in-cultivated-peanut-by-combining-bulked-segregant-analysis-and-high-throughput-sequencing
#14
Galya Kayam, Yael Brand, Adi Faigenboim-Doron, Abhinandan Patil, Ilan Hedvat, Ran Hovav
The growth habit of lateral shoots (also termed "branching habit") is an important descriptive and agronomic character of peanut. Yet, both the inheritance of branching habit and the genetic mechanism that controls it in this crop remain unclear. In addition, the low degree of polymorphism among cultivated peanut varieties hinders fine-mapping of this and other traits in non-homozygous genetic structures. Here, we combined high-throughput sequencing with a well-defined genetic system to study these issues in peanut...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28419131/aflatoxin-levels-in-sunflower-seeds-and-cakes-collected-from-micro-and-small-scale-sunflower-oil-processors-in-tanzania
#15
Juma A Mmongoyo, Felicia Wu, John E Linz, Muraleedharan G Nair, Jovin K Mugula, Robert J Tempelman, Gale M Strasburg
Aflatoxin, a mycotoxin found commonly in maize and peanuts worldwide, is associated with liver cancer, acute toxicosis, and growth impairment in humans and animals. In Tanzania, sunflower seeds are a source of snacks, cooking oil, and animal feed. These seeds are a potential source of aflatoxin contamination. However, reports on aflatoxin contamination in sunflower seeds and cakes are scarce. The objective of the current study was to determine total aflatoxin concentrations in sunflower seeds and cakes from small-scale oil processors across Tanzania...
2017: PloS One
https://www.readbyqxmd.com/read/28413372/electrochemical-impedance-spectroscopy-study-of-concanavalin-a-binding-to-self-assembled-monolayers-of-mannosides-on-gold-wire-electrodes
#16
Jay K Bhattarai, Yih Horng Tan, Binod Pandey, Kohki Fujikawa, Alexei V Demchenko, Keith J Stine
The interactions of the lectin Concanavalin A (Con A) with self-assembled monolayers (SAMs) of thiolated mono-, di-, and tri-mannosides were studied on the surface of gold wires using electrochemical impedance spectroscopy (EIS). The SAMs of mannosides were prepared either pure or along with thiolated triethylene glycol (TEG) at different molar ratios (1:1, 1:2, 1:4, 1:9, and 1:19) to better understand and optimize the interaction conditions. The charge-transfer resistance of the [Fe(CN)6](3-)/(4-) redox probe was compared before and after the interaction at different concentrations of Con A to determine the equilibrium dissociation constant (Kd) and limit of detection (LOD)...
November 1, 2016: Journal of Electroanalytical Chemistry
https://www.readbyqxmd.com/read/28409636/signal-on-photoelectrochemical-immunoassay-for-aflatoxin-b1-based-on-enzymatic-product-etching-mno2-nanosheets-for-dissociation-of-carbon-dots
#17
Youxiu Lin, Qian Zhou, Dianping Tang, Reinhard Niessner, Dietmar Knopp
Aflatoxin B1 (AFB1) monitoring has attracted extensive attention because food safety is a worldwide public health problem. Herein, we design a novel simultaneously visual and photoelectrochemical (PEC) immunosensing system for rapid sensitive detection of AFB1 in foodstuff. The immunoreaction was carried out on anti-AFB1 antibody-modified magnetic beads by using glucose oxidase (GOx)-labeled AFB1-bovine serum albumin (AFB1-BSA) conjugates as the tags with a competitive-type immunoassay format, while the visual and PEC evaluation was performed via carbon quantum dots (CQDs)-functionalized MnO2 nanosheets...
April 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28406451/an-on-site-ultra-sensitive-quantitative-sensing-method-for-the-determination-of-total-aflatoxin-in-peanut-and-rice-based-on-quantum-dot-nanobeads-strip
#18
Suiyan Ouyang, Zhaowei Zhang, Ting He, Peiwu Li, Qi Zhang, Xiaomei Chen, Du Wang, Hui Li, Xiaoqian Tang, Wen Zhang
An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAbAFT) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAbAFT. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1...
April 13, 2017: Toxins
https://www.readbyqxmd.com/read/28400855/ex-vitro-hairy-root-induction-in-detached-peanut-leaves-for-plant-nematode-interaction-studies
#19
Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes, Ana Cristina Miranda Brasileiro
BACKGROUND: Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome studies of wild Arachis species, which harbor resistance to a number of pests and diseases, disclosed several candidate genes for M. arenaria resistance. Peanut is recalcitrant to genetic transformation, so the use of Agrobacterium rhizogenes-derived hairy roots emerged as an alternative for in-root functional characterization of these candidate genes...
2017: Plant Methods
https://www.readbyqxmd.com/read/28397379/b-fahf-2-plus-oral-immunotherapy-oit-is-safer-and-more-effective-than-oit-alone-in-a-murine-model-of-concurrent-peanut-tree-nut-allergy
#20
Kamal D Srivastava, Ying Song, Nan Yang, Changda Liu, Ilan E Goldberg, Anna Nowak-Węgrzyn, Hugh A Sampson, Xiu-Min Li
BACKGROUND: Concurrent sensitization to peanut (PN) and tree nuts (TN), the most dangerous food allergies, is common. Current oral immunotherapy (OIT) is not fully satisfactory. OBJECTIVE: To determine if the herbal formula B-FAHF-2 (BF2) ameliorates PN/TN OIT adverse reactions and enhances persistence of a tolerant state. METHODS: Concurrently sensitized peanut, walnut (WN) and cashew (CSH) allergic mice received 1 day PN/WN/CSH rush OIT plus 3 weeks of maintenance dosing, with or without 3-weeks prior and 3-weeks BF2 co-treatment...
April 11, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
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