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Mirna mitochondria

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https://www.readbyqxmd.com/read/29311649/high-content-image-analysis-reveals-function-of-mir-124-upstream-of-vimentin-in-regulating-motor-neuron-mitochondria
#1
Tal Yardeni, Raquel Fine, Yuvraj Joshi, Tal Gradus-Pery, Noga Kozer, Irit Reichenstein, Eran Yanowski, Shir Nevo, Hila Weiss-Tishler, Michal Eisenberg-Bord, Tal Shalit, Alexander Plotnikov, Haim M Barr, Eran Perlson, Eran Hornstein
microRNAs (miRNAs) are critical for neuronal function and their dysregulation is repeatedly observed in neurodegenerative diseases. Here, we implemented high content image analysis for investigating the impact of several miRNAs in mouse primary motor neurons. This survey directed our attention to the neuron-specific miR-124, which controls axonal morphology. By performing next generation sequencing analysis and molecular studies, we characterized novel roles for miR-124 in control of mitochondria localization and function...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29299142/microrna-34a-promotes-mitochondrial-dysfunction-induced-apoptosis-in-human-lens-epithelial-cells-by-targeting-notch2
#2
Fan Fan, Jianhui Zhuang, Peng Zhou, Xin Liu, Yi Luo
Purpose: Human lens epithelial cell (HLEC) apoptosis is a common pathogenic mechanism in age-related cataracts (ARC). While the function of microRNAs (miRNAs) in the eye is beginning to be explored using miRNA expression array, the role of miR-34a in regulating HLEC apoptosis remains unknown and requires further investigation. Methods: Quantitative reverse-transcript polymerase chain reaction (RT-PCR) was used to determine the expression level of miR-34a in cataractous and control samples...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29246308/mir-3174-contributes-to-apoptosis-and-autophagic-cell-death-defects-in-gastric-cancer-cells-by-targeting-arhgap10
#3
Bowen Li, Lu Wang, Zheng Li, Weizhi Wang, Xiaofei Zhi, Xiaoxu Huang, Qiang Zhang, Zheng Chen, Xuan Zhang, Zhongyuan He, Jianghao Xu, Lu Zhang, Hao Xu, Diancai Zhang, Zekuan Xu
Gastric cancer (GC) is a major health problem worldwide because of its high morbidity and mortality. Considering the well-established roles of miRNA in the regulation of GC carcinogenesis and progression, we screened differentially expressed microRNAs (miRNAs) by using The Cancer Genome Atlas (TCGA) and the GEO databases. We found that miR-3174 was the most significantly differentially expressed miRNA in GC. Ectopic miR-3174 expression was also detected in clinical GC patient samples and cell lines and associated with poor patient prognosis...
December 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29243809/obesity-induced-mitochondrial-dysfunction-in-porcine-adipose-tissue-derived-mesenchymal-stem-cells
#4
Yu Meng, Alfonso Eirin, Xiang-Yang Zhu, Hui Tang, Pritha Chanana, Amir Lerman, Andre J van Wijnen, Lilach O Lerman
BACKGROUND: Transplantation of autologous mesenchymal stem cells (MSCs) may be a viable option for treatment of several diseases. MSCs efficacy depends on adequate function of their mitochondria, which might be impaired in a noxious milieu. OBJECTIVES: We hypothesized that obesity compromises MSCs mitochondrial structure and function, possibly via micro-RNA (miRNA)-based mechanisms. METHODS: MSCs were collected from swine abdominal adipose tissue after 16 weeks of Lean or Obese diet (n = 7 each)...
December 15, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29163823/myc-is-downregulated-by-a-mitochondrial-checkpoint-mechanism
#5
Xiaonan Zhang, Arjan Mofers, Per Hydbring, Maria Hägg Olofsson, Jing Guo, Stig Linder, Padraig D'Arcy
The MYC proto-oncogene serves as a rheostat coupling mitogenic signaling with the activation of genes regulating growth, metabolism and mitochondrial biogenesis. Here we describe a novel link between mitochondria and MYC levels. Perturbation of mitochondrial function using a number of conventional and novel inhibitors resulted in the decreased expression of MYC mRNA. This decrease in MYC mRNA occurred concomitantly with an increase in the levels of tumor-suppressive miRNAs such as members of the let-7 family and miR-34a-5p...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29145655/paternal-exposure-to-environmental-chemical-stress-affectsmale-offspring-s-hepatic-mitochondria
#6
Roger Godschalk, Alex Remels, Camiel Hoogendoorn, Jan van Benthem, Mirjam Luijten, Nur Duale, Gunnar Brunborg, Ann-Karin Olsen, Freek G Bouwman, Armelle Munnia, Marco Peluso, Edwin Mariman, Frederik Jan van Schooten
Pre-conceptional paternal exposures may affect offspring's health, which cannot be explained by mutations in germ cells, but by persistent changes in the regulation of gene expression. Therefore, we investigated whether pre-conceptional paternal exposure to benzo[a]pyrene (B[a]P) could alter the offspring's phenotype. Male C57BL/6 mice were exposed to B[a]P by gavage for 6 weeks, 3x per week, and were crossed with unexposed BALB-c females 6 weeks after the final exposure. The offspring was kept under normal feeding conditions and was sacrificed at 3 weeks of age...
November 14, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29070484/epigenetics-regulates-gene-expression-patterns-of-skeletal-muscle-induced-by-physical-exercise
#7
Chen-Dong Liu, Lu Yang, Hong-Zhou Pu, Qiong Yang, Wen-Yao Huang, Xue Zhao, Li Zhu, Shun-Hua Zhang
As it is well known, proper exercise benefits our mind and body, especially the skeletal muscle. Exercise increases the capacity of muscle metabolism, enhances the biological function of mitochondria, regulates the transformation of muscle fiber types and increases the muscle power. In recent years, more and more researches show that epigenetic regulation plays an important role in strengthening the muscle, and these studies mainly include DNA methylation, histone modification, and regulation of miRNA expression...
October 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/29066618/epigenetic-modification-of-mir-663-controls-mitochondria-to-nucleus-retrograde-signaling-and-tumor-progression
#8
Trevor Carden, Bhupendra Singh, Ved Mooga, Prachi Bajpai, Keshav K Singh
The normal cellular function requires communication between mitochondria and the nucleus, termed mitochondria-to-nucleus retrograde signaling. Disruption of this mechanism has been implicated in the development of cancers. Many proteins are known modulators of retrograde signaling, but whether microRNAs (miRNAs) are also involved is unknown. We conducted an miRNA microarray analysis using RNA from a parental cell line, a Rho0 line lacking mitochondrial DNA (mtDNA) and a Rho0 line with restored mtDNA. We found that miR-663 was down-regulated in the mtDNA-depleted Rho0 line...
December 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29039021/fifty-hertz-magnetic-field-affects-the-epigenetic-modulation-of-the-mir-34b-c-in-neuronal-cells
#9
Claudia Consales, Claudia Cirotti, Giuseppe Filomeni, Martina Panatta, Alessio Butera, Caterina Merla, Vanni Lopresto, Rosanna Pinto, Carmela Marino, Barbara Benassi
The exposure to extremely low-frequency magnetic fields (ELF-MFs) has been associated to increased risk of neurodegenerative diseases, although the underlying molecular mechanisms are still undefined. Since epigenetic modulation has been recently encountered among the key events leading to neuronal degeneration, we here aimed at assessing if the control of gene expression mediated by miRNAs, namely miRs-34, has any roles in driving neuronal cell response to 50-Hz (1 mT) magnetic field in vitro. We demonstrate that ELF-MFs drive an early reduction of the expression level of miR-34b and miR-34c in SH-SY5Y human neuroblastoma cells, as well as in mouse primary cortical neurons, by affecting the transcription of the common pri-miR-34...
October 16, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/29029388/rapamycin-induced-mir-21-promotes-mitochondrial-homeostasis-and-adaptation-in-mtorc1-activated-cells
#10
Hilaire C Lam, Heng-Jia Liu, Christian V Baglini, Harilaos Filippakis, Nicola Alesi, Julie Nijmeh, Heng Du, Alicia Llorente Lope, Katherine A Cottrill, Adam Handen, John M Asara, David J Kwiatkowski, Issam Ben-Sahra, William M Oldham, Stephen Y Chan, Elizabeth P Henske
mTORC1 hyperactivation drives the multi-organ hamartomatous disease tuberous sclerosis complex (TSC). Rapamycin inhibits mTORC1, inducing partial tumor responses; however, the tumors regrow following treatment cessation. We discovered that the oncogenic miRNA, miR-21, is increased in Tsc2-deficient cells and, surprisingly, further increased by rapamycin. To determine the impact of miR-21 in TSC, we inhibited miR-21 in vitro. miR-21 inhibition significantly repressed the tumorigenic potential of Tsc2-deficient cells and increased apoptosis sensitivity...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28942151/molecular-characterization-of-mitochondrial-zucchini-and-its-relation-to-nuage-pirna-pathway-components-in-bombyx-mori-ovary-derived-bmn4-cells
#11
Anandrao Ashok Patil, Tsuneyuki Tatsuke, Hiroaki Mon, Jae Man Lee, Daisuke Morokuma, Masato Hino, Takahiro Kusakabe
Piwi-interacting RNAs (piRNAs) are a class of small non-coding RNAs that associate with PIWI subfamily proteins, which play an important role in transposon silencing in animal germ cell. The piRNAs biogenesis is divided into two major pathways: primary and secondary, and both pathways are independent of double-stranded RNA-processing enzyme Dicer, which processes the single-stranded RNA transcripts in microRNA (miRNA) and siRNA (small interfering RNA) pathway. Primary piRNAs are processed from long non-coding RNA precursors transcribed from piRNA clusters...
November 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28922472/mirna-506-promotes-primary-biliary-cholangitis-like-features-in-cholangiocytes-and-immune-activation
#12
Oihane Erice, Patricia Munoz-Garrido, Javier Vaquero, Maria J Perugorria, Maite G Fernandez-Barrena, Elena Saez, Alvaro Santos-Laso, Ander Arbelaiz, Raul Jimenez-Agüero, Joaquin Fernandez-Irigoyen, Enrique Santamaria, Verónica Torrano, Arkaitz Carracedo, Meenakshisundaram Ananthanarayanan, Marco Marzioni, Jesus Prieto, Ulrich Beuers, Ronald P Oude Elferink, Nicholas F LaRusso, Luis Bujanda, Jose J G Marin, Jesus M Banales
Primary biliary cholangitis (PBC) is a chronic cholestatic liver disease associated with autoimmune phenomena targeting intrahepatic bile duct cells (cholangiocytes). Although PBC etiopathogenesis still remains obscure, development of anti-mitochondrial auto-antibodies against pyruvate dehydrogenase complex-E2 (PDC-E2) is a common feature. MicroRNA (miR) dysregulation occurs in liver and immune cells of PBC patients, but their functional relevance is largely unknown. We previously reported that miR-506 is overexpressed in PBC cholangiocytes and directly targets both Cl(-) /HCO3(-) anion exchanger 2 (AE2) and type III inositol 1,4,5-trisphosphate receptor (InsP3R3), leading to cholestasis...
September 18, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28888871/interplay-of-mitochondria-apoptosis-regulatory-factors-and-micrornas-in-valvular-heart-disease
#13
Muhammad Ishtiaq Jan, Riaz Anwar Khan, Tahir Ali, Muhammad Bilal, Long Bo, Abdul Sajid, Abdul Malik, Naseeb Urehman, Nayyar Waseem, Javed Nawab, Murad Ali, Abdul Majeed, Hamid Ahmad, Sohail Aslam, Sadia Hamera, Aneesa Sultan, Mariam Anees, Qamar Javed, Iram Murtaza
Valvular heart disease (VHD) is an active process involving a wide range of pathological changes. The major complications of VHD are stenosis and regurgitation, which are macroscopic phenomena, induced in part through cellular changes. Altered expression of mitochondria associated genes causes membrane potential depolarization, leading to the increased levels of apoptosis observed in cardiac dysfunction. Objective of this study is to find molecular medicine candidates that can control expression of the key mitochondria apoptosis regulatory genes...
November 1, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28805067/the-lncrna-nespas-is-associated-with-osteoarthritis-progression-and-serves-as-a-potential-new-prognostic-biomarker
#14
Sujung Park, Myeungsoo Lee, Churl-Hong Chun, Eun-Jung Jin
INTRODUCTION: In this article, we explored the hypothesis that the long noncoding RNA, Nespas, promotes osteoarthritis (OA) by supporting abnormal lipid metabolism in human chondrocytes. MATERIALS AND METHODS: Human articular chondrocytes from osteoarthritis patients were used and expression level of Nespas were determined by real-time polymerase chain reaction. Introduction of Nespas and Nespas-associated genes/miRNAs were performed by using a lentiviral system...
August 1, 2017: Cartilage
https://www.readbyqxmd.com/read/28756616/parp-1-par-activity-in-cultured-human-lens-epithelial-cells-exposed-to-two-levels-of-uvb-light
#15
Caroline S Cencer, Shravan K Chintala, Tenira J Townsend, Daniel P Feldmann, Mirna A Awrow, Nahrain A Putris, Mason E Geno, Maria G Donovan, Frank J Giblin
This study investigated poly (ADP-ribose) polymerase-1 (PARP-1) activation in cultured human lens epithelial cells exposed to two levels of UVB light (312 nm peak wavelength), 0.014 and 0.14 J/cm(2) ("low" and "high" dose, respectively). At the low dose, PARP-1 and poly (ADP-ribose) (PAR) polymers acted to repair DNA strand breaks rapidly with no subsequent major effects on either cell morphology or viability. However, following the high UVB dose, there was a dramatic second phase of PARP-1 activation, 90 min later, which included a sudden reappearance of DNA strand breaks, bursts of reactive oxygen species (ROS) formation both within the mitochondria and nucleus, a translocation of PAR from the nucleus to the mitochondria, and an ultimate 70% loss of cell viability occurring after 24 hrs...
July 30, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28740091/microrna-mediated-differential-expression-of-trmu-gtpbp3-and-mto1-in-cell-models-of-mitochondrial-dna-diseases
#16
Salvador Meseguer, Olga Boix, Carmen Navarro-González, Magda Villarroya, Rachid Boutoual, Sonia Emperador, Elena García-Arumí, Julio Montoya, M-Eugenia Armengod
Mitochondrial diseases due to mutations in the mitochondrial (mt) DNA are heterogeneous in clinical manifestations but usually include OXPHOS dysfunction. Mechanisms by which OXPHOS dysfunction contributes to the disease phenotype invoke, apart from cell energy deficit, maladaptive responses to mitochondria-to-nucleus retrograde signaling. Here we used five different cybrid models of mtDNA diseases to demonstrate that the expression of the nuclear-encoded mt-tRNA modification enzymes TRMU, GTPBP3 and MTO1 varies in response to specific pathological mtDNA mutations, thus altering the modification status of mt-tRNAs...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28725638/isolation-of-f-novicida-containing-phagosome-from-infected-human-monocyte-derived-macrophages
#17
Valentina Marecic, Olga Shevchuk, Mateja Ozanic, Mirna Mihelcic, Michael Steinert, Antonija Jurak Begonja, Yousef Abu Kwaik, Marina Santic
Francisella is a gram-negative bacterial pathogen, which causes tularemia in humans and animals. A crucial step of Francisella infection is its invasion of macrophage cells. Biogenesis of the Francisella-containing phagosome (FCP) is arrested for ~15 min at the endosomal stage, followed by gradual bacterial escape into the cytosol, where the microbe proliferates. The crucial step in pathogenesis of tularemia is short and transient presence of the bacterium within phagosome. Isolation of FCPs for further studies has been challenging due to the short period of time of bacterial residence in it and the characteristics of the FCP...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28709769/exploring-the-mitochondrial-microrna-import-pathway-through-polynucleotide-phosphorylase-pnpase
#18
Danielle L Shepherd, Quincy A Hathaway, Mark V Pinti, Cody E Nichols, Andrya J Durr, Shruthi Sreekumar, Kristen M Hughes, Seth M Stine, Ivan Martinez, John M Hollander
Cardiovascular disease is the primary cause of mortality for individuals with type 2 diabetes mellitus. During the diabetic condition, cardiovascular dysfunction can be partially attributed to molecular changes in the tissue, including alterations in microRNA (miRNA) interactions. MiRNAs have been reported in the mitochondrion and their presence may influence cellular bioenergetics, creating decrements in functional capacity. In this study, we examined the roles of Argonaute 2 (Ago2), a protein associated with cytosolic and mitochondrial miRNAs, and Polynucleotide Phosphorylase (PNPase), a protein found in the inner membrane space of the mitochondrion, to determine their role in mitochondrial miRNA import...
July 11, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28706939/chlamydia-and-mitochondria-an-unfragmented-relationship
#19
COMMENT
Suvagata R Chowdhury, Thomas Rudel
Presence of pathogens within a eukaryotic cell is apt to generate stress. Such stress eventually leads to host defense responses, which includes, but is not limited to, apoptosis induction and subsequent destruction of the host cell and the pathogen. Obligate intracellular pathogens such as Chlamydia trachomatis are dependent on the survival of the host cell owing to their unique replication niche within a membrane-bound inclusion. Furthermore, being energy parasites, chlamydial development is strictly dependent on the host metabolism...
June 14, 2017: Microbial Cell
https://www.readbyqxmd.com/read/28701783/circulating-mir-323-3p-is-a-biomarker-for-cardiomyopathy-and-an-indicator-of-phenotypic-variability-in-friedreich-s-ataxia-patients
#20
M Seco-Cervera, D González-Rodríguez, J S Ibáñez-Cabellos, L Peiró-Chova, P González-Cabo, E García-López, J J Vílchez, I Sanz-Gallego, F V Pallardó, J L García-Giménez
MicroRNAs (miRNAs) are noncoding RNAs that contribute to gene expression modulation by regulating important cellular pathways. In this study, we used small RNA sequencing to identify a series of circulating miRNAs in blood samples taken from Friedreich's ataxia patients. We were thus able to develop a miRNA biomarker signature to differentiate Friedreich's ataxia (FRDA) patients from healthy people. Most research on FDRA has focused on understanding the role of frataxin in the mitochondria, and a whole molecular view of pathological pathways underlying FRDA therefore remains to be elucidated...
July 12, 2017: Scientific Reports
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