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https://www.readbyqxmd.com/read/29778081/molecular-aspect-of-silver-nanoparticles-regulated-embryonic-development-in-zebrafish-danio-rerio-by-oct-4-expression
#1
Biplab Sarkar, Suresh K Verma, Javed Akhtar, Surya Prakash Netam, Sanjay Kr Gupta, Pritam Kumar Panda, Koel Mukherjee
With the enhancement of commercial manifestation of silver nanoparticles, concerned has risen on their accumulation in aquatic system and consequent effects on fish development and metabolism. In this study, experiments were conducted to assess the impacts of silver nanoparticles on early life cycles of fish considering Zebrafish (Danio rerio) as experimental model. Silver nanoparticles were synthesized through chemical reduction method and characterized through UV-visible spectroscopy, dynamic light scattering (DLS), and HR-TEM...
May 4, 2018: Chemosphere
https://www.readbyqxmd.com/read/29776312/on-the-prospects-of-using-high-intensity-thz-pulses-to-induce-ultrafast-temperature-jumps-in-liquid-water
#2
Pankaj Kr Mishra, Vincent Bettaque, Oriol Vendrell, Robin Santra, Ralph Welsch
Ultrashort, high-intensity THz pulses, e.g., created at free-electron laser facilities, allow for direct investigation as well as the driving of intermolecular modes in liquids like water and thus will deepen our understanding of the hydrogen bonding network. In this work, the temperature-jump (T-jump) of water induced by THz radiation is simulated for ten different THz frequencies in the range from 3 to 30 THz and five different pulse intensities in the range from 1 × 1011 to 5 × 1012 W/cm2 employing both ab initio molecular dynamics (AIMD) and force field molecular dynamics (FFMD) approaches...
May 18, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29775789/e-coli-encoding-blandm-5-associated-with-community-acquired-uti-cases-with-unusual-mic-creep-like-phenomenon-against-imipenem
#3
Varsha Rani Gajamer, Amitabha Bhattacharjee, Deepjyoti Paul, Chandrayee Deshamukhya, Ashish Kr Singh, Nilu Pradhan, Hare Krishna Tiwari
OBJECTIVES: Carbapenemase-producing Escherichia coli are of major clinical concern. The present study aimed to identify NDM-5 producing E.coli associated with community-acquired urinary tract infection, co-harboring ESBL genes and a pattern of imipenem MIC creep. METHODS: A total of 973 urine samples were collected from females of age between 18-49 diagnosed with UTI. Isolates were identified by standard microbiological procedures. Presence of blaNDM and ESBL genes was determined by PCR assay and sequencing...
May 15, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29758273/in-silico-experiment-system-for-testing-hypothesis-on-gene-functions-using-three-condition-specific-biological-networks
#4
Chai-Jin Lee, Dongwon Kang, Sangseon Lee, Sunwon Lee, Jaewoo Kang, Sun Kim
Determining functions of a gene requires time consuming, expensive biological experiments. Scientists can speed up this experimental process if the literature information and biological networks can be adequately provided. In this paper, we present a web-based information system that can perform in silico experiments of computationally testing hypothesis on the function of a gene. A hypothesis that is specified in English by the user is converted to genes using a literature and knowledge mining system called BEST...
May 11, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29756265/web-based-thyroid-imaging-reporting-and-data-system-malignancy-risk-of-atypia-of-undetermined-significance-or-follicular-lesion-of-undetermined-significance-thyroid-nodules-calculated-by-a-combination-of-ultrasonography-features-and-biopsy-results
#5
Young Jun Choi, Jung Hwan Baek, Jung Hee Shin, Woo Hyun Shim, Seon-Ok Kim, Won-Hong Lee, Dong Eun Song, Tae Yong Kim, Ki-Wook Chung, Jeong Hyun Lee
BACKGROUND: The purpose of this study was to construct a web-based predictive model using ultrasound characteristics and subcategorized biopsy results for thyroid nodules of atypia of undetermined significance/follicular lesion of undetermined significance (AUS/FLUS) to stratify the risk of malignancy. METHODS: Data included 672 thyroid nodules from 656 patients from a historical cohort. We analyzed ultrasound images of thyroid nodules and biopsy results according to nuclear atypia and architectural atypia...
May 13, 2018: Head & Neck
https://www.readbyqxmd.com/read/29756078/engineering-endothelialized-microfluidics-for-investigating-vascular-and-hematologic-processes-using-non-traditional-fabrication-techniques
#6
Robert G Mannino, Navaneeth Kr Pandian, Abhishek Jain, Wilbur A Lam
Investigating the complex interplay between blood cells and the endothelium is crucial in understanding the pathophysiology of many diseases. Observation of the in vivo vasculature is difficult due to the complexities of vessel geometry, limited visualization capability, as well as variability and complexity inherent to biologic systems. Therefore, in vitro systems serve as ideal tools to study these cellular interactions. Microfluidic technologies are an ideal tool for recapitulating the vasculature in vivo as they can be used to fabricate fluidic channels on the size scale capillaries using gas permeable, biologically inert, and optically transparent substrates...
March 2018: Current Opinion in Biomedical Engineering
https://www.readbyqxmd.com/read/29751643/the-effect-of-lean-seafood-and-non-seafood-diets-on-fasting-and-postprandial-serum-metabolites-and-lipid-species-results-from-a-randomized-crossover-intervention-study-in-healthy-adults
#7
Mette Schmedes, Claudia Balderas, Eli Kristin Aadland, Hélène Jacques, Charles Lavigne, Ingvild Eide Graff, Øyvin Eng, Asle Holthe, Gunnar Mellgren, Jette Feveile Young, Ulrik Kræmer Sundekilde, Bjørn Liaset, Hanne Christine Bertram
The metabolic effects associated with intake of different dietary protein sources are not well characterized. We aimed to elucidate how two diets that varied in main protein sources affected the fasting and postprandial serum metabolites and lipid species. In a randomized controlled trial with crossover design, healthy adults ( n = 20) underwent a 4-week intervention with two balanced diets that varied mainly in protein source (lean-seafood versus non-seafood proteins). Nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry analyses were applied to examine the effects of the two diets on serum metabolites...
May 11, 2018: Nutrients
https://www.readbyqxmd.com/read/29748162/cocreated-smartphone-app-to-improve-the-quality-of-life-of-adolescents-and-young-adults-with-cancer-kr%C3%A3-ftv%C3%A3-rket-protocol-for-a-quantitative-and-qualitative-evaluation
#8
Abbey Elsbernd, Maiken Hjerming, Camilla Visler, Lisa Lyngsie Hjalgrim, Carsten Utoft Niemann, Kirsten Boisen, Helle Pappot
BACKGROUND: Adolescents and young adults with cancer face significant challenges during the course of their medical treatment and recovery from illness. Many adolescents and young adults struggle with long-term complications in the physical, psychosocial, economic, and academic domains. Mobile health (mHealth) interventions provide an innovative platform for delivering supportive care, particularly through the utilization of apps on smartphones and tablets. To create a successful mHealth intervention for adolescents and young adults, youth input and feedback is essential...
May 10, 2018: JMIR Research Protocols
https://www.readbyqxmd.com/read/29746660/interspia-a-web-application-for-exploring-the-dynamics-of-protein-protein-interactions-among-multiple-species
#9
Daehong Kwon, Daehwan Lee, Juyeon Kim, Jongin Lee, Mikang Sim, Jaebum Kim
Proteins perform biological functions through cascading interactions with each other by forming protein complexes. As a result, interactions among proteins, called protein-protein interactions (PPIs) are not completely free from selection constraint during evolution. Therefore, the identification and analysis of PPI changes during evolution can give us new insight into the evolution of functions. Although many algorithms, databases and websites have been developed to help the study of PPIs, most of them are limited to visualize the structure and features of PPIs in a chosen single species with limited functions in the visualization perspective...
May 9, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29743778/respiratory-morbidity-among-rice-mill-workers-in-an-urban-area-of-burdwan-district-west-bengal-a-cross-sectional-study
#10
Madhab Ch Rana, Somnath Naskar, Ramaprasad Roy, Dilip Kr Das, Soumya Das
Background: Rice mill workers constitute a special group from the perspective of occupational health. Unprotected dust exposure among them adversely affects their respiratory health, which needs to be evaluated. Adequate evidence is still lacking in many parts of India including West Bengal. Burdwan is one of the main rice-producing districts in the state with abundant rice mills. Aim: The aim of the study was to find out the prevalence and pattern of respiratory morbidity and associated background characteristics of rice mill workers...
January 2018: Indian Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/29737589/ll-37-fragments-have-antimicrobial-activity-against-staphylococcus-epidermidis-biofilms-and-wound-healing-potential-in-hacat-cell-line
#11
Paola Saporito, Michelle Vang Mouritzen, Anders Løbner-Olesen, Håvard Jenssen
Staphylococcus epidermidis is a common nosocomial pathogen able to form biofilms in indwelling devices, resulting in chronic infections, which are refractory to antibiotics treatment. Staphylococcal biofilms are also associated with the delayed reepithelization and healing of chronic wounds. The human cathelicidin peptide LL-37 has been proven active against S. epidermidis biofilms in vitro and to promote wound healing. As previous studies have demonstrated that fragments of LL-37 could possess an equal antibacterial activity as the parent peptide, we tested whether shorter (12-mer) synthetic fragments of LL-37 maintained the antibiofilm and/or immune modulating activity, aiming at the identification of essential regions within the LL-37 parent sequence...
May 8, 2018: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/29736860/resonance-bonding-in-xngy-x-f-cl-br-i-ng-kr-or-xe-y-cn-or-nc-molecules-an-nbo-nrt-investigation
#12
Junjie Song, Yue Su, Yanping Jia, Lusheng Chen, Guiqiu Zhang
Several noble-gas-containing molecules XNgY were observed experimentally. However, the bonding in such systems is still not understood. Using natural bond orbital and natural resonance theory (NBO/NRT) methods, the present work investigated bonding of the title molecules. The results show that each of the studied XNgY molecules should be better described as a resonance hybrid of ω-bonding and [Formula: see text]-type long-bonding structures: X:- Ng+  - Y, X - Ng+ : Y- , and X^ Y. The ω-bonding and long-bonding make competing contributions to the composite resonance hybrid due to the accurately preserved bond order conservation principle...
May 7, 2018: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29729484/high-efficiency-dual-cycle-conversion-system-using-kr-85
#13
Mark A Prelas, Modeste Tchakoua Tchouaso
This paper discusses the use of one of the safest isotopes known isotopes, Kr-85, as a candidate fuel source for deep space missions. This isotope comes from 0.286% of fission events. There is a vast quantity of Kr-85 stored in spent fuel and it is continually being produced by nuclear reactors. In using Kr-85 with a novel Dual Cycle Conversion System (DCCS) it is feasible to boost the system efficiency from 26% to 45% over a single cycle device while only increasing the system mass by less than 1%. The Kr-85 isotope is the ideal fuel for a Photon Intermediate Direct Energy Conversion (PIDEC) system...
April 26, 2018: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/29726944/gemiccl-mining-genotype-and-expression-data-of-cancer-cell-lines-with-elaborate-visualization
#14
Inhae Jeong, Namhee Yu, Insu Jang, Yukyung Jun, Min-Seo Kim, Jinhyuk Choi, Byungwook Lee, Sanghyuk Lee
GEMiCCL is available at https://www.kobic.kr/GEMICCL/.
January 1, 2018: Database: the Journal of Biological Databases and Curation
https://www.readbyqxmd.com/read/29725305/development-of-a-high-throughput-flow-cytometry-assay-to-monitor-defective-trafficking-and-rescue-of-long-qt2-mutant-herg-channels
#15
Scott A Kanner, Ananya Jain, Henry M Colecraft
Long QT Syndrome (LQTS) is an acquired or inherited disorder characterized by prolonged QT interval, exertion-triggered arrhythmias, and sudden cardiac death. One of the most prevalent hereditary LQTS subtypes, LQT2, results from loss-of-function mutations in the hERG channel, which conducts I Kr , the rapid component of the delayed rectifier K+ current, critical for cardiac repolarization. The majority of LQT2 mutations result in Class 2 deficits characterized by impaired maturation and trafficking of hERG channels...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29724279/instrument-for-assessing-knowledge-of-oral-anticoagulant-therapy-construction-and-validation
#16
Fernanda Souza E Silva Garcia, Débora Cristina Prévide Teixeira da Cunha, Rafaela de Oliveira Manzato, Fabiana Bolela, Pedro Alberto Barbetta, Rosana Spadoti Dantas
BACKGROUND AND PURPOSE: A valid and reliable assessment tool is fundamental for evaluating the knowledge of individuals about using oral anticoagulant therapy (OAT). The purpose of this study was to develop a tool to assess knowledge about OAT and provide evidence to support its use in people living in developing countries. METHODS: Construction and validation: development of items; assessment of face and content validity; pilot study; application of the instrument to a sample of 500 users of OAT to analyze dimensionality, convergent validation, and reliability...
April 1, 2018: Journal of Nursing Measurement
https://www.readbyqxmd.com/read/29719076/dissecting-the-role-of-kr%C3%A3-ppel-homolog-1-in-the-metamorphosis-and-female-reproduction-of-the-cotton-bollworm-helicoverpa-armigera
#17
W-N Zhang, L Ma, C Liu, L Chen, H-J Xiao, G-M Liang
In insects, metamorphosis and reproduction are controlled by juvenile hormone (JH) and 20-hydroxyecdysone (20E). Krüppel homolog 1 (Kr-h1), a transcription factor, is regarded as a JH-early inducible gene responsible for the repression of metamorphosis. However, the role of Kr-h1 in reproduction of holometabolic insects is relatively less understood. In this study, we studied the role of Kr-h1 in larvae-pupae transition and female reproduction in the major agricultural pest Helicoverpa armigera. Two HaKr-h1 isoforms (HaKr-h1α and HaKr-h1ß) were identified, with HaKr-h1α predominant in the cotton bollworm...
May 2, 2018: Insect Molecular Biology
https://www.readbyqxmd.com/read/29716608/comparison-of-the-virulence-of-three-h3n2-canine-influenza-virus-isolates-from-korea-and-china-in-mouse-and-guinea-pig-models
#18
Xing Xie, Woonsung Na, Aram Kang, Minjoo Yeom, Heejun Yuk, Hyoungjoon Moon, Sung-Jae Kim, Hyun-Woo Kim, Jeong-Ki Kim, Maoda Pang, Yongshan Wang, Yongjie Liu, Daesub Song
BACKGROUND: Avian-origin H3N2 canine influenza virus (CIV) has been the most common subtype in Korea and China since 2007. Here, we compared the pathogenicity and transmissibility of three H3N2 CIV strains [Chinese CIV (JS/10), Korean CIV (KR/07), and Korean recombinant CIV between the classic H3N2 CIV and the pandemic H1N1 virus (MV/12)] in BALB/c mouse and guinea pig models. The pandemic H1N1 (CA/09) strain served as the control. RESULTS: BALB/c mice infected with H1N1 had high mortality and obvious body weight loss, whereas no overt disease symptoms were observed in mice inoculated with H3N2 CIV strains...
May 2, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29716360/cryocooler-based-test-setup-for-high-current-applications
#19
Jedidiah Pradhan, Nisith Kr Das, Anindya Roy, Anjan Duttagupta
A cryo-cooler based cryogenic test setup has been designed, fabricated, and tested. The setup incorporates two numbers of cryo-coolers, one for sample cooling and the other one for cooling the large magnet coil. The performance and versatility of the setup has been tested using large samples of high-temperature superconductor magnet coil as well as short samples with high current. Several un-calibrated temperature sensors have been calibrated using this system. This paper presents the details of the system along with results of different performance tests...
April 2018: Review of Scientific Instruments
https://www.readbyqxmd.com/read/29716200/stereodynamics-of-ne-3-p-2-reacting-with-ar-kr-xe-and-n-2
#20
Junwen Zou, Sean D S Gordon, Silvia Tanteri, Andreas Osterwalder
Stereodynamics experiments of Ne(3 P2 ) reacting with Ar, Kr, Xe, and N2 leading to Penning and associative ionization have been performed in a crossed molecular beam apparatus. A curved magnetic hexapole was used to state-select and polarize Ne(3 P2 ) atoms which were then oriented in a rotatable magnetic field and crossed with a beam of Ar, Kr, Xe, or N2 . The ratio of associative to Penning ionization was recorded as a function of the magnetic field direction for collision energies between 320 cm-1 and 500 cm-1 ...
April 28, 2018: Journal of Chemical Physics
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