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https://www.readbyqxmd.com/read/27911483/effects-of-dietary-heme-iron-and-exercise-training-on-abdominal-fat-accumulation-and-lipid-metabolism-in-high-fat-diet-fed-mice
#1
Masanori Katsumura, Shoko Takagi, Hana Oya, Shohei Tamura, Takaoki Saneyasu, Kazuhisa Honda, Hiroshi Kamisoyama
Animal by-products can be recycled and used as sources of essential nutrients. Water-soluble heme iron (WSHI), a functional food additive for supplementing iron, is produced by processing animal blood. In this study, we investigated the effects of dietary supplementation of 3% WSHI and exercise training for 4 weeks on the accumulation of abdominal fat and lipid metabolism in mice fed high-fat diet. Exercise-trained mice had significantly less perirenal adipose tissue, whereas WSHI-fed mice tended to have less epididymal adipose tissue...
December 2, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/27911360/development-of-a-more-sensitive-and-specific-chromogenic-agar-medium-for-the-detection-of-vibrio-parahaemolyticus-and-other-vibrio-species
#2
Marie Yeung, Trevor Thorsen
Foodborne infections in the US caused by Vibrio species have shown an upward trend. In the genus Vibrio, V. parahaemolyticus is responsible for the majority of Vibrio-associated infections. Thus, accurate differentiation among Vibrio spp. and detection of V. parahaemolyticus is critically important to ensure the safety of our food supply. Although molecular techniques are increasingly common, culture-depending methods are still routinely done and they are considered standard methods in certain circumstances...
November 8, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910018/fluorescence-in-situ-hybridization-probe-validation-for-clinical-use
#3
Jun Gu, Janice L Smith, Patricia K Dowling
In this chapter, we provide a systematic overview of the published guidelines and validation procedures for fluorescence in situ hybridization (FISH) probes for clinical diagnostic use. FISH probes-which are classified as molecular probes or analyte-specific reagents (ASRs)-have been extensively used in vitro for both clinical diagnosis and research. Most commercially available FISH probes in the United States are strictly regulated by the U.S. Food and Drug Administration (FDA), the Centers for Disease Control and Prevention (CDC), the Centers for Medicare & Medicaid Services (CMS) the Clinical Laboratory Improvement Amendments (CLIA), and the College of American Pathologists (CAP)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909880/il-9-producing-cells-in-the-development-of-ige-mediated-food-allergy
#4
REVIEW
Dana Shik, Sunil Tomar, Jee-Boong Lee, Chun-Yu Chen, Andrew Smith, Yui-Hsi Wang
Food allergy is a harmful immune reaction driven by uncontrolled type 2 immune responses. Considerable evidence demonstrates the key roles of mast cells, IgE, and TH2 cytokines in mediating food allergy. However, this evidence provides limited insight into why only some, rather than all, food allergic individuals are prone to develop life-threatening anaphylaxis. Clinical observations suggest that patients sensitized to food through the skin early in life may later develop severe food allergies. Aberrant epidermal thymic stromal lymphopoietin and interleukin (IL) 33 production and genetic predisposition can initiate an allergic immune response mediated by dendritic cells and CD4(+)TH2 cells in inflamed skin...
December 1, 2016: Seminars in Immunopathology
https://www.readbyqxmd.com/read/27908547/high-dietary-choline-and-betaine-intake-is-associated-with-low-insulin-resistance-in-the-newfoundland-population
#5
Xiang Gao, Yongbo Wang, Guang Sun
OBJECTIVE: Dietary betaine supplement could ameliorate insulin resistance (IR) in animals, but no data are available for choline. Reports on humans are rare. The aim of this study was to investigate the association between dietary choline and betaine intake and IR in humans. METHODS: We assessed 2394 adults from the CODING (Complex Diseases in the Newfoundland population: Environment and Genetics) study. Intake of dietary choline and betaine was evaluated from the Willett Food Frequency Questionnaire...
January 2017: Nutrition
https://www.readbyqxmd.com/read/27906992/a-dual-filtration-based-multiplex-pcr-method-for-simultaneous-detection-of-viable-escherichia-coli-o157-h7-listeria-monocytogenes-and-staphylococcus-aureus-on-fresh-cut-cantaloupe
#6
Ke Feng, Wenzhong Hu, Aili Jiang, Sarengaowa, Yongping Xu, Yu Zou, Liu Yang, Xin Wang
Fresh-cut cantaloupe is particularly susceptible to contamination with pathogenic bacteria, such as Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus. Therefore, development of rapid, yet accurate detection techniques is necessary to ensure food safety. In this study, a multiplex PCR system and propidium monoazide (PMA) concentration were optimized to detect all viable pathogens in a single tube. A dual filtration system utilized a filtration membrane with different pore sizes to enrich pathogens found on fresh-cut cantaloupe...
2016: PloS One
https://www.readbyqxmd.com/read/27906472/long-term-clinical-impact-and-cost-effectiveness-of-obeticholic-acid-for-the-treatment-of-primary-biliary-cholangitis
#7
Sumeyye Samur, Matthew Klebanoff, Reiner Banken, Daniel S Pratt, Rick Chapman, Daniel A Ollendorf, Anne M Loos, Kathleen Corey, Chin Hur, Jagpreet Chhatwal
: Primary biliary cholangitis (PBC) is a chronic, progressive autoimmune liver disease that mainly affects middle-aged women. Obeticholic acid (OCA), which was recently approved by the Food and Drug Administration for PBC treatment, has demonstrated positive effects on biochemical markers of liver function. Our objective was to evaluate the long-term clinical impact and cost-effectiveness of OCA as a second-line treatment for PBC in combination with ursodeoxycholic acid (UDCA) in adults with an inadequate response to UDCA...
November 7, 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/27906139/myxobacteria-mediated-synthesis-of-silver-nanoparticles-and-their-impregnation-in-wrapping-paper-used-for-enhancing-shelf-life-of-apples
#8
Swati Bhople, Swapnil Gaikwad, Swapna Deshmukh, Shital Bonde, Aniket Gade, Sanjib Sen, Anna Brezinska, Hanna Dahm, Mahendra Rai
The authors report Myxobacteria virescens (M. virescens) mediated synthesis of silver nanoparticles (AgNPs) and its efficacy against Staphylococcus aureus (ATCC-33591), Salmonella typhi (ATCC-51812), Escherichia coli (E. coli) (ATCC-14948), Klebsiella pneumoniae (MTCC-4030) and Pseudomonas aeruginosa (MTCC-4673). The organism exhibiting resistance to various antibiotics showed remarkable sensitivity, when used in combination of antibiotics and AgNPs. Antimicrobial property of AgNPs is playing a significant role in medicine and food storage...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27905961/estimating-average-alcohol-consumption-in-the-population-using-multiple-sources-the-case-of-spain
#9
Luis Sordo, Gregorio Barrio, María J Bravo, Joan R Villalbí, Albert Espelt, Montserrat Neira, Enrique Regidor
BACKGROUND: National estimates on per capita alcohol consumption are provided regularly by various sources and may have validity problems, so corrections are needed for monitoring and assessment purposes. Our objectives were to compare different alcohol availability estimates for Spain, to build the best estimate (actual consumption), characterize its time trend during 2001-2011, and quantify the extent to which other estimates (coverage) approximated actual consumption. METHODS: Estimates were: alcohol availability from the Spanish Tax Agency (Tax Agency availability), World Health Organization (WHO availability) and other international agencies, self-reported purchases from the Spanish Food Consumption Panel, and self-reported consumption from population surveys...
June 2, 2016: Population Health Metrics
https://www.readbyqxmd.com/read/27905857/recent-development-in-sample-preparation-and-analytical-techniques-for-determination-of-quinolone-residues-in-food-products
#10
Zhichao Zhang, Hefa Cheng
Used heavily in food animal production, quinolones occur widely in food products of animal origin. Development of highly sensitive and selective analytical techniques for the detection of quinolone residues, often at trace levels, in food samples is necessary to ensure food safety and understand their public health risk. With complex matrices, food samples typically require a series of pretreatment steps, such as powdering, homogenization, deproteinization, and filtration. This review summarizes the recent advances in extraction, concentration, and detection techniques for quinolones in food product samples, and briefly compares the advantages and limitations of major techniques...
December 1, 2016: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/27904396/moisture-sensitivity-optical-mechanical-and-structural-properties-of-whey-protein-based-edible-films-incorporated-with-rapeseed-oil
#11
Sabina Galus, Justyna Kadzińska
The objective of this work is to study the effect of the rapeseed oil content on the physical properties of whey protein emulsion films. For this purpose, whey protein films with the addition of 0, 1, 2 and 3% of rapeseed oil, and glycerol as a plasticizer were obtained by the casting method. Film-forming emulsions were evaluated and compared using light scattering granulometry. The Sauter mean diameters (d32) of lipid droplets in film-forming solutions showed an increasing trend when increasing the oil volume fractions...
March 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27904080/an-ic-ms-ms-method-for-the-determination-of-1-hydroxyethylidene-1-1-diphosphonic-acid-on-uncooked-foods-treated-with-peracetic-acid-based-sanitizers
#12
Ippei Suzuki, Hiroki Kubota, Takashi Ohtsuki, Chiye Tatebe, Atsuko Tada, Takeo Yano, Hiroshi Akiyama, Kyoko Sato
A rapid, sensitive, and specific analytical method for the determination of 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) on uncooked foods after treatment with a peracetic acid-based sanitizer (PAS) was developed. The method involves simple sample preparation steps and analysis using ion chromatography (IC) coupled with tandem mass spectrometry (MS/MS). The quantification limits of HEDP on uncooked foods are 0.007 mg/kg for vegetables and fruits and 0.2 mg/kg for meats. The recovery and relative standard deviation (RSD) of HEDP analyses of uncooked foods ranged from 73...
2016: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27903745/inhibiting-microglia-expansion-prevents-diet-induced-hypothalamic-and-peripheral-inflammation
#13
Caroline André, Omar Guzman-Quevedo, Charlotte Rey, Julie Rémus-Borel, Samantha Clark, Ashley Castellanos-Jankiewicz, Elodie Ladeveze, Thierry Leste-Lasserre, Agnes Nadjar, Djoher Nora Abrous, Sophie Laye, Daniela Cota
Cell proliferation and neuroinflammation in the adult hypothalamus may contribute to the pathogenesis of obesity. Here we tested whether the intertwining of these two processes has a role in the metabolic changes caused by three weeks of saturated high-fat diet (HFD) consumption.As compared to chow, HFD-fed mice rapidly increased body weight and fat mass, and specifically showed increased microglia number in the arcuate nucleus (ARC) of the hypothalamus. Microglia expansion required the adequate presence of fats and carbohydrates in the diet, since feeding mice a very high-fat, very low-carbohydrate diet did not affect cell proliferation...
November 30, 2016: Diabetes
https://www.readbyqxmd.com/read/27902940/a-novel-lab-on-chip-platform-with-integrated-solid-phase-pcr-and-supercritical-angle-fluorescence-saf-microlens-array-for-highly-sensitive-and-multiplexed-pathogen-detection
#14
Tran Quang Hung, Wai Hoe Chin, Yi Sun, Anders Wolff, Dang Duong Bang
Solid-phase PCR (SP-PCR) has become increasingly popular for molecular diagnosis and there have been a few attempts to incorporate SP-PCR into lab-on-a-chip (LOC) devices. However, their applicability for on-line diagnosis is hindered by the lack of sensitive and portable on-chip optical detection technology. In this paper, we addressed this challenge by combining the SP-PCR with super critical angle fluorescence (SAF) microlens array embedded in a microchip. We fabricated miniaturized SAF microlens array as part of a microfluidic chamber in thermoplastic material and performed multiplexed SP-PCR directly on top of the SAF microlens array...
November 12, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27902869/drivers-of-interannual-variability-in-virioplankton-abundance-at-the-coastal-western-antarctic-peninsula-and-the-potential-effects-of-climate-change
#15
Claire Evans, Joost Brandsma, David W Pond, Hugh J Venables, Michael P Meredith, Harry J Witte, Sharon Stammerjohn, William H Wilson, Andrew Clarke, Corina P D Brussaard
An eight year time-series in the Western Antarctic Peninsula (WAP) with an approximately weekly sampling frequency was used to elucidate changes in virioplankton abundance and their drivers in this climatically-sensitive region. Virioplankton abundances at the coastal WAP show a pronounced seasonal cycle with interannual variability in the timing and magnitude of the summer maxima. Bacterioplankton abundance is the most influential driving factor of the virioplankton, and exhibit closely coupled dynamics. Sea ice cover and duration predetermine levels of phytoplankton stock and thus, influence virioplankton by dictating the substrates available to the bacterioplankton...
November 30, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27901297/climate-change-alters-the-reproductive-phenology-and-investment-of-a-lacustrine-fish-the-three-spine-stickleback
#16
Rachel A Hovel, Stephanie M Carlson, Thomas P Quinn
High-latitude lakes are particularly sensitive to the effects of global climate change, demonstrating earlier ice breakup, longer ice-free seasons, and increased water temperatures. Such physical changes have implications for diverse life-history traits in taxa across entire lake food webs. Here, we use a five-decade time series from an Alaskan lake to explore effects of climate change on growth and reproduction of a widely distributed lacustrine fish, the three-spine stickleback (Gasterosteus aculeatus). We used multivariate autoregressive state-space (MARSS) models to describe trends in the mean length for multiple size classes and to explore the influence of physical (date of ice breakup, surface water temperature) and biological (density of con- and heterospecifics) factors...
November 30, 2016: Global Change Biology
https://www.readbyqxmd.com/read/27896827/leaping-through-the-looking-glass-secondary-analysis-of-the-effect-of-skin-test-size-and-age-of-introduction-on-peanut-tolerance-after-early-peanut-introduction
#17
Matthew Greenhawt, David Fleischer, Edmond S Chan, Carina Venter, David Stukus, Ruchi Gupta, Jonathan Spergel
BACKGROUND: In the Learning Early About Peanut Allergy (LEAP) study, early peanut introduction in high-risk 4-11 month olds was associated with a significantly decreased risk of developing peanut allergy. However, the influences of key baseline high-risk factors on peanut tolerance are poorly understood. METHODS: Secondary analysis was conducted on the publically available LEAP dataset, exploring relationships between peanut tolerance, baseline peanut/egg sensitization, eczema severity/duration, age of introduction, gender, and race...
November 29, 2016: Allergy
https://www.readbyqxmd.com/read/27895288/some-biological-activities-of-pigments-extracted-from-micrococcus-roseus-ptcc-1411-and-rhodotorula-glutinis-ptcc-5257
#18
Hossein Rostami, Hassan Hamedi, Mahmoud Yolmeh
The importance of replacing synthetic pigments with natural types is increasing day by day in the food industry due to the harmful effects of some synthetic pigments. Microorganisms are a major source of natural pigments, which nowadays have attracted the attention of researchers. In this study, carotenoid pigments were produced by Micrococcus roseus and Rhodotorula glutinis, and some of their biological properties such as antimicrobial, antioxidant, anticancer, and anti-inflammatory activities were evaluated...
December 2016: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/27895108/the-effect-of-dietary-fish-oil-on-weight-gain-and-insulin-sensitivity-is-dependent-on-apoe-genotype-in-humanized-targeted-replacement-mice
#19
Kenna E Slim, David Vauzour, Noemi Tejera, Peter J Voshol, Aedin Cassidy, Anne Marie Minihane
We investigated the independent and interactive impact of the common APOE genotype and marine n-3 polyunsaturated fatty acids (PUFA) on the development of obesity and associated cardiometabolic dysfunction in a murine model. Human APOE3 and APOE4 targeted replacement mice were fed either a high-fat control diet (HFD) or a HFD supplemented with 3% n-3 PUFA from fish oil (HFD + FO) for 8 wk. We established the impact of intervention on food intake, bodyweight, and visceral adipose tissue (VAT) mass; plasma, lipids (cholesterol and triglycerides), liver enzymes, and adipokines; glucose and insulin during an intraperitoneal glucose tolerance test; and Glut4 and ApoE expression in VAT...
November 28, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27894746/roux-en-y-gastric-bypass-versus-calorie-restriction-support-for-surgery-per-se-as-the-direct-contributor-to-altered-responses-of-insulin-and-incretins-to-a-mixed-meal
#20
Johan Berggren, Andreas Lindqvist, Jan Hedenbro, Leif Groop, Nils Wierup
OBJECTIVE: To study the immediate effects of Roux-en-Y gastric bypass (RYGB) on glucose homeostasis, insulin, and incretin responses to mixed-meal tests compared with the effects of calorie restriction (CR). SETTING: University-affiliated bariatric surgery clinic. BACKGROUND: RYGB induces remission of type 2 diabetes (T2D) long before significant weight loss occurs. The time course and underlying mechanisms of this remission remain enigmatic...
September 28, 2016: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
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