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https://www.readbyqxmd.com/read/29333503/the-effect-of-a-vegan-versus-aha-diet-in-coronary-artery-disease-evade-cad-trial-study-design-and-rationale
#1
Binita Shah, Lisa Ganguzza, James Slater, Jonathan D Newman, Nicole Allen, Edward Fisher, John Larigakis, Francisco Ujueta, Eugenia Gianos, Yu Guo, Kathleen Woolf
Background: Multiple studies demonstrate the benefit of a vegan diet on cardiovascular risk factors when compared to no intervention or usual dietary patterns. The aim of this study is to evaluate the effect of a vegan diet versus the American Heart Association (AHA)-recommended diet on inflammatory and glucometabolic profiles in patients with angiographically defined coronary artery disease (CAD). Study Design: This study is a randomized, open label, blinded end-point trial of 100 patients with CAD as defined by ≥50% diameter stenosis in a coronary artery ≥2 mm in diameter on invasive angiography...
December 2017: Contemporary Clinical Trials Communications
https://www.readbyqxmd.com/read/29333341/preventive-effects-of-salvia-officinalis-leaf-extract-on-insulin-resistance-and-inflammation-in-a-model-of-high-fat-diet-induced-obesity-in-mice-that-responds-to-rosiglitazone
#2
Mohamed R Ben Khedher, Mohamed Hammami, Jonathan R S Arch, David C Hislop, Dominic Eze, Edward T Wargent, Małgorzata A Kępczyńska, Mohamed S Zaibi
Background: Salvia officinalis (sage) is a native plant to the Mediterranean region and has been used for a long time in traditional medicine for various diseases. We investigated possible anti-diabetic, anti-inflammatory and anti-obesity effects of sage methanol (MetOH) extract in a nutritional mouse model of obesity, inflammation and insulin resistance, as well as its effects on lipolysis and lipogenesis in 3T3-L1 cells. Methods: Diet-induced obese (DIO) mice were treated for five weeks with sage methanol extract (100 and 400 mg kg-1/day bid), or rosiglitazone (3 mg kg-1/day bid), as a positive control...
2018: PeerJ
https://www.readbyqxmd.com/read/29332838/rapid-and-sensitive-tapered-capillary-microextraction-combined-to-on-line-sample-stacking-capillary-electrophoresis-for-extraction-and-quantification-of-two-beta-blockers-in-human-urine
#3
Shucheng Sun, Yujia Wang, Xin Liu, Rao Fu, Li Yang
A tapered-capillary microextraction (tCap-μEx) combining with field-amplified stacking (FASI) method for CE analysis was developed. The tCap-μEx method is based on the construction of a micro solid phase extraction (SPE) column by narrowing the end of a silica capillary from 530µm (inner diameter) to 20µm, enabling the packing of 45µm sorbent particles without a frit. Various parameters that may affect the microextraction and FASI-CE analysis have been investigated and optimized. This study shows that microextraction exhibits advantages of small sample and sorbent volumes (less than 200μL sample and 2μL sorbent) and fast extraction time of 6min...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332837/a-novel-electroanalytical-assay-for-sulfamethazine-determination-in-food-samples-based-on-conducting-polymer-nanocomposite-modified-electrodes
#4
Ya-Ling Su, Shu-Hua Cheng
The toxicity of sulfa drugs has attracted great attention, and the reported electrochemical methods for sulfa drugs usually employ a high oxidation potential. In this work, a one-pot synthesized conducting polymer nanocomposite containing poly(3,4-ethylenedioxythiophene) (PEDOT) and MnO2 was cast on a screen-printed carbon electrode (SPCE), and the modified electrode showed superior electrochemical activity over a bare electrode for sulfamethazine (SMZ) determination. The SMZ detection was based on the electrochemical oxidation product, which showed an adsorptive property and exhibited a redox couple at 0...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332815/single-particle-analysis-of-tio2-in-candy-products-using-triple-quadrupole-icp-ms
#5
S Candás-Zapico, D J Kutscher, M Montes-Bayón, J Bettmer
Titanium dioxide (TiO2) belongs to the materials that have gained great importance in many applications. In its particulate form (micro- or nanoparticles), it has entered a huge number of consumer products and food-grade TiO2, better known as E171 within the European Union, represents an important food additive. Thus, there is an increasing need for analytical methods able to detect and quantify such particles. In this regard, inductively coupled-mass spectrometry (ICP-MS), in particular single particle ICP-MS (spICP-MS), has gained importance due to its simplicity and ease of use...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332786/recent-advances-and-developments-on-integrating-nanotechnology-with-chemiluminescence-assays
#6
REVIEW
Ashish Tiwari, S J Dhoble
Chemiluminescence (CL) techniques are extensively utilized for detection of analytes due to their high sensitivity, rapidity and selectivity. With the advent of nanotechnology and incorporation of the nanoparticles in the CL system has revolutionized the assays due to their unique optical and mechanical properties. Several CL-based reactions have been developed where these nanoparticle based CL sensors have evolved as excellent prospects for sensing in various analytical applications. This review article addresses the nanoparticles based CL detection system that are recently developed, the mechanisms has been summarized and the role of luminophors have been discussed...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332677/pilot-study-of-sleep-and-meal-timing-effects-independent-of-sleep-duration-and-food-intake-on-insulin-sensitivity-in-healthy-individuals
#7
Theresa Pizinger, Kyle Kovtun, Arindam RoyChoudhury, Blandine Laferrère, Ari Shechter, Marie-Pierre St-Onge
This pilot study tested the independent and interactive effects of sleep and meal times, under identical sleep duration and feeding conditions, on insulin sensitivity (Si) in overweight adults. Participants underwent a 4-phase randomized crossover inpatient study differing in sleep times: normal (Ns: 0000-0800 hours) or late (Ls: 0330-1130 hours); and in meal times: normal (Nm: 1, 5, 11, and 12.5 hours after awakening) or late (Lm: 4.5, 8.5, 14.5, and 16 hours after awakening). An insulin-modified frequently sampled intravenous glucose tolerance test, at scheduled breakfast time, and a meal tolerance test, at scheduled lunch time, were performed to assess Si after 3 days in each condition...
February 2018: Sleep Health
https://www.readbyqxmd.com/read/29332097/is-the-atopy-patch-test-reliable-in-the-evaluation-of-food-allergy-related-atopic-dermatitis
#8
Mahboubeh Mansouri, Elham Rafiee, Sepideh Darougar, Mehrnaz Mesdaghi, Zahra Chavoshzadeh
BACKGROUND: Aeroallergens and food allergens are found to be relevant in atopic dermatitis. The atopy patch test (APT) can help to detect food allergies in children with atopic dermatitis. This study evaluates if the APT is a valuable tool in the diagnostic workup of children with food allergy-related atopic dermatitis. METHODS: 42 children between 6 months and 12 years of age were selected at the Mofid Children Hospital. Atopic dermatitis was diagnosed, and the severity of the disease was determined...
January 13, 2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29331959/probenecid-improves-cardiac-function-in-patients-with-heart-failure-with-reduced-ejection-fraction-in-vivo-and-cardiomyocyte-calcium-sensitivity-in-vitro
#9
Nathan Robbins, Mark Gilbert, Mohit Kumar, James W McNamara, Patrick Daly, Sheryl E Koch, Ginger Conway, Mohamed Effat, Jessica G Woo, Sakthivel Sadayappan, Jack Rubinstein
BACKGROUND: Transient receptor potential vanilloid 2 is a calcium channel activated by probenecid. Probenecid is a Food and Drug Administration-approved uricosuric drug that has recently been shown to induce positive lusitropic and inotropic effects in animal models through cardiomyocyte transient receptor potential vanilloid 2 activation. The aim of this study was to test the hypothesis that oral probenecid can improve cardiac function and symptomatology in patients with heart failure with reduced ejection fraction and to further elucidate its calcium-dependent effects on myocyte contractility...
January 13, 2018: Journal of the American Heart Association
https://www.readbyqxmd.com/read/29331816/new-robust-sensitive-fluorescence-spectroscopy-coupled-with-plsr-for-estimation-of-quercetin-in-ziziphus-mucronata-and-ziziphus-sativa
#10
Javid Hussain, Fazal Mabood, Ahmed Al-Harrasi, Liaqat Ali, Tania Shamim Rizvi, Farah Jabeen, Syed Abdullah Gilani, Shehla Shinwari, Mushtaq Ahmad, Zahra Khalfan Alabri, Said Hamood Salim Al Ghawi
Flavonoids are natural antioxidants derived from plants and commonly found in a variety of foods to sequester free radicals. Quercetin, belonging to flavonol subclass of flavonoids, has received considerable attention because of its wide uses as a nutritional supplement as well as a phytochemical remedy for a number of diseases. In the current study, quantification of quercetin was carried out in two medicinally important flavonoid rich plant Ziziphus mucronata and Ziziphus sativa. Emission spectroscopy was utilized as a new method coupled with Partial Least Squares Regression (PLSR) and the cross validation was done by UV-Visible spectroscopy...
January 6, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29331617/asthma-predictive-index-in-relation-to-respiratory-mechanics-by-impulse-oscillometry-in-recurrent-wheezers
#11
T Arikoglu, S B Batmaz, D D Yildirim, Ö Tezol, G Bozlu, S Kuyucu
BACKGROUND: The identification of children who will have persistent asthma has become a focus of recent research. The aim of this study was to assess whether impulse oscillometry (IOS) has a diagnostic value to predict modified API (asthma predictive index) in pre-schoolers with recurrent wheezing. METHODS: Pre-school children aged 3-6 years with recurrent wheezing were enrolled. The study population was divided into two groups based on mAPI criteria. Lung function was assessed by IOS...
January 10, 2018: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/29331514/employment-of-proteomic-and-immunological-based-methods-for-the-identification-of-catalase-as-novel-allergen-from-banana
#12
Jasna Nikolić, Andrijana Nešić, Skadi Kull, Frauke Schocker, Uta Jappe, Marija Gavrović-Jankulović
Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminata) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase...
January 10, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29331368/in-vitro-and-in-vivo-safety-analysis-of-enterococcus-faecium-2c-isolated-from-human-breast-milk
#13
Soodabeh Khalkhali, Naheed Mojgani
INTRODUCTION: Safety analysis of probiotic bacteria is an obligatory characteristic to be evaluated prior to application in food or pharmacological products. This study was designed to evaluate in vitro and in vivo safety parameters of Enterococcus faecium 2C strain, a probiotic candidate isolated from human breast milk. MATERIAL AND METHODS: E.faecium 2C was studied for its hemolytic activity and phenotypic antibiotics resistance profile. In vivo safety of the mentioned Enterococcus strain was studied by determining acute oral toxicity in Wistar Male rats...
January 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29329874/a-survey-of-free-glutamic-acid-in-foods-using-a-robust-lc-ms-ms-method
#14
Nur Cebi, Canan Ekinci Dogan, Elmas Oktem Olgun, Osman Sagdic
An effective and simultaneous liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used with the aim of quantifying monosodium glutamate (MSG) in foodstuffs, such as chips, taste cubes, sauces and soups. The results were linear (R2 = 1), with very low LOD and LOQ values, 1.0 µg/kg, 5.0 µg/kg, respectively. Excellent repeatability and reproducibility were also achieved. This highly sensitive and robust LC-MS/MS technique was applied successfully for the detection and quantification of MSG in a wide variety of foodstuffs...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29329857/meat-species-identification-using-dna-luminol-interaction-and-their-slow-diffusion-onto-the-biochip-surface
#15
Nura Fazira Noor Azam, Sharmili Roy, Syazana Abdullah Lim, Minhaz Uddin Ahmed
Recently, there has been a growing concern of consumers on the authenticity of food ingredients including adulteration with porcine and/or its derivatives. Therefore, this work reports on the development of a novel, simple, sensitive and rapid luminol-based electrochemiluminescence (ECL) technique for Sus scrofa (Porcine) DNA detection. Porcine DNA was firstly amplified using loop-mediated isothermal amplification (LAMP) method and subsequently added to luminol solution for further ECL analysis for quantification...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29328973/feeding-inhibition-in-corbicula-fluminea-o-f-muller-1774-as-an-effect-criterion-to-pollutant-exposure-perspectives-for-ecotoxicity-screening-and-refinement-of-chemical-control
#16
Bruno Branco Castro, Carlos Silva, Inês Patrunilho Efe Macário, Bruno Oliveira, Fernando Gonçalves, Joana Luísa Pereira
Bivalves are commonly used in biomonitoring programs to track pollutants. Several features, including its filter-feeding abilities, cumulatively argue in favour of the use of the Asian clam (Corbicula fluminea) as a biosentinel and an ecotoxicological model. Filtration in bivalves is very sensitive to external stimuli and its control is dictated by regulation of the opening/closure of the valves, which may be used as an avoidance defence against contaminants. Here, we investigate the filter-feeding behaviour of the Asian clam as an endpoint for assessing exposure to pollutants, driven by two complementary goals: (i) to generate relevant and sensitive toxicological information based on the ability of C...
January 4, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29328913/an-integrated-view-of-immunometabolism
#17
REVIEW
Yun Sok Lee, Joshua Wollam, Jerrold M Olefsky
The worldwide obesity epidemic has emerged as a major cause of insulin resistance and Type 2 diabetes. Chronic tissue inflammation is a well-recognized feature of obesity, and the field of immunometabolism has witnessed many advances in recent years. Here, we review the major features of our current understanding with respect to chronic obesity-related inflammation in metabolic tissues and focus on how these inflammatory changes affect insulin sensitivity, insulin secretion, food intake, and glucose homeostasis...
January 11, 2018: Cell
https://www.readbyqxmd.com/read/29325863/future-high-flux-assays-on-pathogenic-virulent-and-toxic-genetics-from-various-pathogens
#18
REVIEW
Yanmei Li, Caiying Bai, Ling Yang, Jie Fu, Muxia Yan, Dingqiang Chen, Li Zhang
Toxins, encoding by virulence factors, are significant cause of food-borne illnesses and death in the worldwide. Loop-mediated isothermal amplification (LAMP) is one of the widely used methodologies because of the high sensitivity, specificity and rapidity. Nowadays, LAMP has been regarded as an innovative gene amplification technology and emerged as an alternative to PCR-based methodologies in identification of the pathogenic virulent and toxic genetics. The high sensitivity of LAMP enables detection of the pathogens in sample materials even without time consuming and sample preparation...
January 8, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29325171/maternal-and-child-health-handbook-use-for-maternal-and-child-care-a-cluster-randomized-controlled-study-in-rural-java-indonesia
#19
Keiko Osaki, Tomoko Hattori, Akemi Toda, Erna Mulati, Lukas Hermawan, Kirana Pritasari, Saptawati Bardosono, Soewarta Kosen
Background: Effectiveness of the Maternal and Child Health Handbook (MCHHB), a home-based booklet for pregnancy, delivery and postnatal/child health, was evaluated on care acquisition and home care in rural Java, a low service-coverage area. Methods: We conducted a health centre-based randomized trial, with a 2-year follow-up. Intervention included (i) MCHHB provision at antenatal care visits; (ii) records and guides by health personnel on and with the MCHHB; and (iii) sensitization of care by volunteers using the MCHHB...
January 9, 2018: Journal of Public Health
https://www.readbyqxmd.com/read/29324685/novel-spectroscopic-and-electrochemical-sensors-and-nanoprobes-for-the-characterization-of-food-and-biological-antioxidants
#20
REVIEW
Reşat Apak, Sema Demirci Çekiç, Ayşem Üzer, Saliha Esin Çelik, Mustafa Bener, Burcu Bekdeşer, Ziya Can, Şener Sağlam, Ayşe Nur Önem, Erol Erçağ
Since an unbalanced excess of reactive oxygen/nitrogen species (ROS/RNS) causes various diseases, determination of antioxidants that can counter oxidative stress is important in food and biological analyses. Optical/electrochemical nanosensors have attracted attention in antioxidant activity (AOA) assessment because of their increased sensitivity and selectivity. Optical sensors offer advantages such as low cost, flexibility, remote control, speed, miniaturization and on-site/in situ analysis. Electrochemical sensors using noble metal nanoparticles on modified electrodes better catalyze bioelectrochemical reactions...
January 11, 2018: Sensors
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