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https://www.readbyqxmd.com/read/29149712/detection-of-perfluoroalkyl-acids-and-sulphonates-in-italian-eel-samples-by-hplc-hrms-orbitrap
#1
Luca Maria Chiesa, Maria Nobile, Elisa Pasquale, Claudia Balzaretti, Petra Cagnardi, Doriana Tedesco, Sara Panseri, Francesco Arioli
Perfluoroalkyl substances (PFASs) contain one or more carbon-bound hydrogens substituted by fluorine. Since the 1950s, these compounds have been used to manufacture fat- and water-resistant fabrics, paper and food containers, and to produce photographic films, firefighting foams, detergents and insecticides. The widespread use and global distribution of PFASs, have led to their accumulation in the environment. Food, particularly fish and other seafood, is considered the main route of human exposure to PFASs...
October 27, 2017: Chemosphere
https://www.readbyqxmd.com/read/29149627/a-simple-and-sensitive-two-tube-multiplex-pcr-assay-for-simultaneous-detection-of-ten-meat-species
#2
Olga V Prusakova, Xenia A Glukhova, Gaida V Afanas'eva, Yulia A Trizna, Ludmila F Nazarova, Igor P Beletsky
A sensitive and reliable technique for meat species identification is required to prevent food adulteration, particularly in meat production. This work developed an optimized multiplex PCR assay for simultaneous identification of five commonly consumed and five commonly banned meat species in meat products. We designed primers that specifically amplified mitochondrial ATPase subunit 8 gene regions of different lengths of bovine, ovine, swine, chicken, turkey, cat, dog, mouse and human DNAs. The developed multiplex PCR assay proved to be specific, sensitive down to 30pg DNA per reaction, reproducible and economical...
October 26, 2017: Meat Science
https://www.readbyqxmd.com/read/29148163/development-and-validation-of-a-high-performance-liquid-chromatography-method-for-determination-of-lisinopril-in-human-plasma-by-magnetic-solid-phase-extraction-and-pre-column-derivatization
#3
Noushin Rastkari, Reza Ahmadkhaniha
A sensitive, reliable and simple HPLC method was developed for the determination of lisinopril in human plasma. The method consists of extraction and clean-up steps based on magnetic solid-phase extraction and pre-column derivatization with a fluorescent reagent. The mobile phase consisted of a mixture of methanol-sodium dihydrogen phosphate (pH 3.0; 0.005 m; 75:25, v/v). The flow rate was set at 0.7 mL/min. Fluorescence detection was performed at 470nm excitation and 530nm emission wavelengths. Total chromatography run time was 5 min...
November 16, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29146657/a-randomised-controlled-trial-of-a-duodenal-jejunal-bypass-sleeve-device-endobarrier-compared-with-standard-medical-therapy-for-the-management-of-obese-subjects-with-type-2-diabetes-mellitus
#4
Michael Alan Glaysher, Aruchuna Mohanaruban, Christina Gabriele Prechtl, Anthony P Goldstone, Alexander Dimitri Miras, Joanne Lord, Navpreet Chhina, Emanuela Falaschetti, Nicholas Andrew Johnson, Werd Al-Najim, Claire Smith, Jia V Li, Mayank Patel, Ahmed R Ahmed, Michael Moore, Neil Poulter, Stephen Bloom, Ara Darzi, Carel Le Roux, James P Byrne, Julian P Teare
INTRODUCTION: The prevalence of obesity and obesity-related diseases, including type 2 diabetes mellitus (T2DM), is increasing. Exclusion of the foregut, as occurs in Roux-en-Y gastric bypass, has a key role in the metabolic improvements that occur following bariatric surgery, which are independent of weight loss. Endoscopically placed duodenal-jejunal bypass sleeve devices, such as the EndoBarrier (GI Dynamics, Lexington, Massachusetts, USA), have been designed to create an impermeable barrier between chyme exiting the stomach and the mucosa of the duodenum and proximal jejunum...
November 15, 2017: BMJ Open
https://www.readbyqxmd.com/read/29146502/protracted-motivational-dopamine-related-deficits-following-adolescence-sugar-overconsumption
#5
Fabien Naneix, Florence Darlot, Véronique De Smedt-Peyrusse, Jean-Rémi Pape, Etienne Coutureau, Martine Cador
Adolescence represents a critical period characterized by major neurobiological changes. Chronic stimulation of the reward system during adolescence might constitute an important factor of vulnerability to pathological development. Increasing evidences suggest that adolescent overconsumption of sweet palatable foods impact reward-based processes. However, the neurobiological bases of these deficits remain poorly understood. Previous studies have demonstrated motivational deficits for palatable foods after sweet diet exposure during adolescence that might involve the dopamine (DA) system, a central actor in incentive processes...
November 13, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29146202/effects-of-polystyrene-microplastics-on-early-stages-of-two-marine-invertebrates-with-different-feeding-strategies
#6
Silvia Messinetti, Silvia Mercurio, Marco Parolini, Michela Sugni, Roberta Pennati
Nowadays, microplastics represent one of the main threats to marine ecosystems, being able to affect organisms at different stages of their life cycle and at different levels of the food web. Although the presence of plastic debris has been reported in different habitats and the ability to ingest it has been confirmed for different taxa, few studies have been performed to elucidate the effects on survival and development of marine animals. Thus, we explored the effects of different environmental concentrations of polystyrene microbeads on the early stages of two invertebrate species widespread in the Mediterranean shallow waters: the pelagic planktotrophic pluteus larvae of the sea urchin Paracentrotus lividus and the filter-feeding sessile juveniles of the ascidian Ciona robusta...
November 13, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29145897/it-does-matter-where-you-come-from-mothers-experiences-of-childbirth-in-midwife-obstetric-units-tshwane-south-africa
#7
Sarie J Oosthuizen, Anne-Marie Bergh, Robert C Pattinson, Jackie Grimbeek
BACKGROUND: Health professionals are striving to improve respectful care for women, but they fall short in the domains of effective communication, respectful and dignified care and emotional support during labour. This study aimed to determine women's experiences of childbirth with a view to improving respectful clinical care practices in low-risk, midwife-led obstetric units in the Tshwane District Health District, South Africa. METHODS: A survey covering all midwife-led units in the district was conducted among 653 new mothers...
November 16, 2017: Reproductive Health
https://www.readbyqxmd.com/read/29145563/neuronal-dnmt1-deficiency-attenuates-diet-induced-obesity-in-mice
#8
Emily C Bruggeman, John T Garretson, Rui Wu, Hang Shi, Bingzhong Xue
Aberrant neuronal DNA methylation patterns have been implicated in the promotion of obesity development, but the role of neuronal DNA methyltransferases (Dnmts; enzymes that catalyze DNA methylation) in energy balance remains poorly understood. We investigated whether neuronal Dnmt1 regulates normal energy homeostasis and obesity development using a novel Dnmt1 knockout mouse model, Dnmt1fl/fl Synapsin1Cre (ND1KO), which specifically deletes Dnmt1 in neurons.Neuronal Dnmt1 deficiency reduced adiposity in chow-fed mice and attenuated obesity in HFD-fed male mice...
November 14, 2017: Endocrinology
https://www.readbyqxmd.com/read/29145391/household-food-production-is-positively-associated-with-dietary-diversity-and-intake-of-nutrient-dense-foods-for-older-preschool-children-in-poorer-families-results-from-a-nationally-representative-survey-in-nepal
#9
Prajula Mulmi, William A Masters, Shibani Ghosh, Grace Namirembe, Ruchita Rajbhandary, Swetha Manohar, Binod Shrestha, Keith P West, Patrick Webb
BACKGROUND: Nutrition-sensitive interventions supporting enhanced household food production have potential to improve child dietary quality. However, heterogeneity in market access may cause systematic differences in program effectiveness depending on household wealth and child age. Identifying these effect modifiers can help development agencies specify and target their interventions. OBJECTIVE: This study investigates mediating effects of household wealth and child age on links between farm production and child diets, as measured by production and intake of nutrient-dense food groups...
2017: PloS One
https://www.readbyqxmd.com/read/29145213/depressed-immune-responses-and-accelerated-splenic-apoptosis-due-to-experience-of-food-deprivation-and-inequality-but-not-unstable-social-status-in-balb-c-mice
#10
Marjan Aghajani, Mohammad Reza Vaez Mahdavi, Mohsen Khalili Najafabadi, Tooba Ghazanfari, Fatemeh Moradi, Ravieh Golchoobian, Hasan Askari, Nima Sanadgol, Ehsan Kazemi Moghaddam
OBJECTIVE(S): We aimed to show that the immune system is sensitive to the detrimental effects of inequality and social injustice, and splenic vulnerability to apoptosis may also increase. METHODS: In order of better determination of immune responses to chronic social stress, we implemented food deprivation, food intake inequality, and unstable social status (a change of cage-mate every 3 days) for a period of 14 days in 60 male Balb/c mice. At the end of this stress period, nitric oxide (NO) production by peritoneal adherent cells and the serum concentration of corticosterone were measured...
November 17, 2017: Neuroimmunomodulation
https://www.readbyqxmd.com/read/29145201/dietary-fructo-oligosaccharides-attenuate-early-activation-of-cd4-t-cells-which-produce-both-th1-and-th2-cytokines-in-the-intestinal-lymphoid-tissues-of-a-murine-food-allergy-model
#11
Masato Tsuda, Haruka Arakawa, Narumi Ishii, Chihiro Ubukata, Mana Michimori, Masanari Noda, Kyoko Takahashi, Shuichi Kaminogawa, Akira Hosono
BACKGROUND: Fructo-oligosaccharides (FOS) are prebiotic agents with immunomodulatory effects involving improvement of the intestinal microbiota and metabolome. In this study, we investigated the cellular mechanisms through which FOS modulate intestinal antigen-specific CD4+ T cell responses in food allergy, using OVA23-3 mice. METHODS: OVA23-3 mice were fed an experimental diet containing either ovalbumin (OVA) or OVA and FOS for 1 week. Body weight and mucosal mast cell protease 1 in the serum were measured as the indicator of intestinal inflammation...
November 17, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29145095/fear-of-food-prospectively-predicts-drive-for-thinness-in-an-eating-disorder-sample-recently-discharged-from-intensive-treatment
#12
Cheri A Levinson, Leigh C Brosof, Jackie Ma, Laura Fewell, Eric J Lenze
Fears of food are common in individuals with eating disorders and contribute to the high relapse rates. However, it is unknown how fears of food contribute to eating disorder symptoms across time, potentially contributing to an increased likelihood of relapse. Participants diagnosed with an eating disorder (N=168) who had recently completed intensive treatment were assessed after discharge and one month later regarding fear of food, eating disorder symptoms, anxiety sensitivity, and negative affect. Cross lagged path analysis was utilized to determine if fear of food predicted subsequent eating disorder symptoms one month later...
November 9, 2017: Eating Behaviors
https://www.readbyqxmd.com/read/29144387/neighborhood-prices-of-healthier-and-unhealthier-foods-and-associations-with-diet-quality-evidence-from-the-multi-ethnic-study-of-atherosclerosis
#13
David M Kern, Amy H Auchnicloss, Mark F Stehr, Ana V Diez Roux, Latetia V Moore, Genevieve P Kanter, Lucy F Robinson
It is known that the price of food influences the purchasing and consumption decisions of individuals; however, little work has examined if the price of healthier food relative to unhealthier food in an individual's neighborhood is associated with overall dietary quality while using data from multiple regions in the United States. Cross-sectional person-level data came from The Multi-Ethnic Study of Atherosclerosis (exam 5, 2010-2012 n = 2765); a food frequency questionnaire assessed diet. Supermarket food/beverage prices came from Information Resources Inc...
November 16, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29144215/a-filtration-based-rapid-test-using-a-partially-purified-third-stage-larval-antigen-to-detect-specific-antibodies-for-the-diagnosis-of-gnathostomiasis
#14
A Ma, Y Wang, X L Liu, H M Zhang, P Eamsobhana, H S Yong, X X Gan
Human gnathostomiasis is an emerging food-borne parasitic disease caused by nematodes of the genus Gnathostoma. Currently, serological tests are commonly applied to support clinical diagnosis. In the present study, a simple and rapid filtration-based test, dot immune-gold filtration assay (DIGFA) was developed using a partially purified antigen of Gnathostoma third-stage larvae (L3). A total of 180 serum samples were tested to evaluate the diagnostic potential of DIGFA for gnathostomiasis. The diagnostic sensitivity and specificity were 96...
November 16, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/29143878/sensitive-mass-spectrometric-assay-for-determination-of-15-deoxy-%C3%AE-12-14-prostaglandin-j2-and-its-application-in-human-plasma-samples-of-patients-with-diabetes
#15
Jakob Morgenstern, Thomas Fleming, Ivelina Kadiyska, Sebastian Brings, Jan Benedikt Groener, Peter Nawroth, Markus Hecker, Maik Brune
The determination of individual prostaglandins (PG) in humans is mainly performed in urine samples. The quantification of PGs in human plasma could improve the understanding of particular PG species under various physiological and pathological conditions. 15-Deoxy-Δ(12,14)-prostaglandin J2 (15d-PGJ2) is a dehydrated downstream product of PGD2 and is of high interest due to its recently discovered anti-inflammatory effects. Increasing availability of highly sensitive mass spectrometry allows the quantification of low abundant biomarkers like 15d-PGJ2 in human plasma samples...
November 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29143385/patterns-of-allergic-sensitization-and-atopic-dermatitis-from-1-to-3-years-effects-on-allergic-diseases
#16
C Dharma, D L Lefebvre, M M Tran, W Y W Lou, P Subbarao, A B Becker, P J Mandhane, S E Turvey, M R Sears
BACKGROUND: While allergic sensitization and atopic dermatitis (AD) are known to increase the risk for allergic diseases, the impact of different temporal and clinical patterns of sensitization and AD is less well defined. OBJECTIVE: We investigated patterns of sensitization and AD from early infancy to age 3, and the differential risk for developing allergic diseases within each pattern in a general cohort. METHODS: Children (n=2629) from the Canadian Healthy Infant Longitudinal Development (CHILD) Study underwent skin prick tests and were assessed clinically for AD at ages 1 and 3 years...
November 16, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29142517/is-methylation-analysis-of-sfrp2-tfpi2-ndrg4-and-bmp3-promoters-suitable-for-colorectal-cancer-screening-in-the-korean-population
#17
Soo-Kyung Park, Hae Lim Baek, Junghee Yu, Ji Yeon Kim, Hyo-Joon Yang, Yoon Suk Jung, Kyu Yong Choi, Hungdai Kim, Hyung Ook Kim, Kyung Uk Jeong, Ho-Kyung Chun, Kyungeun Kim, Dong Il Park
Background/Aims: Colorectal cancer (CRC) screening using stool DNA was recently found to yield good detection rates. A multi-target stool DNA test (Cologuard®, Exact Sciences), including methylated genes has been recently approved by the U.S. Food and Drug Administration. The aim of this study was to validate these aberrantly methylated genes as stool-based DNA markers for detecting CRC and colorectal advanced adenoma (AA) in the Korean population. Methods: A single-center study was conducted in 36 patients with AA; 35 patients with CRC; and 40 endoscopically diagnosed healthy controls using CRC screening colonoscopy...
October 2017: Intestinal Research
https://www.readbyqxmd.com/read/29142467/non-celiac-gluten-sensitivity-all-wheat-attack-is-not-celiac
#18
REVIEW
Samuel O Igbinedion, Junaid Ansari, Anush Vasikaran, Felicity N Gavins, Paul Jordan, Moheb Boktor, Jonathan S Alexander
Currently, 1% of the United States population holds a diagnosis for celiac disease (CD), however, a more recently recognized and possibly related condition, "non-celiac gluten sensitivity" (NCGS) has been suggested to affect up to 6% of the United States public. While reliable clinical tests for CD exist, diagnosing individuals affected by NCGS is still complicated by the lack of reliable biomarkers and reliance upon a broad set of intestinal and extra intestinal symptoms possibly provoked by gluten. NCGS has been proposed to exhibit an innate immune response activated by gluten and several other wheat proteins...
October 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29141979/measurement-errors-in-dietary-assessment-using-self-reported-24-hour-recalls-in-low-income-countries-and-strategies-for-their-prevention
#19
Rosalind S Gibson, U Ruth Charrondiere, Winnie Bell
Securing accurate measurements of dietary intake across populations is challenging. Of the methods, self-reported 24-h recalls are often used in low-income countries (LICs) because they are quick, culturally sensitive, do not require high cognitive ability, and provide quantitative data on both foods and nutrients. Measuring intakes via 24-h recalls involves 1) collecting data on food intakes, 2) the appropriate use of relevant food-composition data for calculating nutrient intakes, and 3) statistically converting observed intakes to "usual intakes" for evaluating nutrient adequacy or relations between foods and nutrients and health outcomes...
November 2017: Advances in Nutrition
https://www.readbyqxmd.com/read/29141603/detect-i-detect-ii-a-study-protocol-for-a-prospective-multicentre-observational-study-to-validate-the-uromark-assay-for-the-detection-of-bladder-cancer-from-urinary-cells
#20
Wei Shen Tan, Andrew Feber, Liqin Dong, Rachael Sarpong, Sheida Rezaee, Simon Rodney, Pramit Khetrapal, Patricia de Winter, Frelyn Ocampo, Rumana Jalil, Norman R Williams, Chris Brew-Graves, John D Kelly
BACKGROUND: Haematuria is a common finding in general practice which requires visual inspection of the bladder by cystoscopy as well as upper tract imaging. In addition, patients with non-muscle invasive bladder cancer (NMIBC) often require surveillance cystoscopy as often as three monthly depending on disease risk. However, cystoscopy is an invasive procedure which is uncomfortable, requires hospital attendance and is associated with a risk of urinary tract infection. We have developed the UroMark assay, which can detect 150 methylation specific alteration specific to bladder cancer using DNA from urinary sediment cells...
November 15, 2017: BMC Cancer
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