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https://www.readbyqxmd.com/read/28342912/peanut-oleosins-associated-with-severe-peanut-allergy-importance-of-lipophilic-allergens-for-comprehensive-allergy-diagnostics
#1
Christian Schwager, Skadi Kull, Jochen Behrends, Niels Röckendorf, Frauke Schocker, Andreas Frey, Arne Homann, Wolf-Meinhard Becker, Uta Jappe
BACKGROUND: Peanut allergy is one of the most common and most severe food allergies in Western countries and its accurate diagnosis to prevent potential life-threatening allergic reactions is crucial. However, aqueous extracts used for routine diagnostic measurements are devoid of lipophilic allergens such as oleosins. We have recently succeeded in the isolation and purification of these unique proteins, and the current study evaluates their allergenic potential and clinical relevance...
March 22, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28342836/membrane-fatty-acid-composition-as-a-determinant-of-listeria-monocytogenes-sensitivity-to-trans-cinnamaldehyde
#2
Gil Rogiers, Biniam T Kebede, Ann Van Loey, Chris W Michiels
Trans-cinnamaldehyde, the major compound of cinnamon essential oil, is a potentially interesting natural antimicrobial food preservative. Although a number of studies have addressed its mode of action, the factors that determine bacterial sensitivity or tolerance to trans-cinnamaldehyde are poorly understood. We report the detailed characterization of a Listeria monocytogenes Scott A trans-cinnamaldehyde hypersensitive mutant defective in IlvE, which catalyzes the reversible transamination of branched-chain amino acids to the corresponding short-chain α-ketoacids...
March 23, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28342765/hippocampal-insulin-resistance-and-altered-food-decision-making-as-players-on-obesity-risk
#3
REVIEW
Amanda Brondani Mucellini, Natasha Kim de Oliveira da Fonseca, Gisele Gus Manfro, Patrícia Pelufo Silveira
There are increasing evidences that hippocampus can modulate the decision of what, when and how much to eat, in addition to its already recognized role in learning and memory processes. Insulin also has been linked to brain functions such as feeding behavior and the imbalance of its mechanism of action on hippocampus is being related to cognitive dysfunction. The discussion here is whether changes in insulin action could contribute to intake dysregulation and obesogenic behavior as a primary consequence of impairing hippocampal functioning, aside from the role of this hormone on obesity development through peripheral metabolic pathways...
March 22, 2017: Neuroscience and Biobehavioral Reviews
https://www.readbyqxmd.com/read/28339990/detecting-thresholds-for-bitter-umami-and-sweet-tastants-in-broiler-chicken-using-a-2-choice-test-method
#4
Shira L Cheled-Shoval, Naama Reicher, Masha Y Niv, Zehava Uni
The sense of taste has a key role in nutrient sensing and food intake in animals. A standardized and simple method for determination of tastant-detection thresholds is required for chemosensory research in poultry. We established a 24-h, 2-alternative, forced-choice solution-consumption method and applied it to measure detection thresholds for 3 G-protein-coupled receptor-mediated taste modalities-bitter, sweet, and umami-in chicken. Four parameters were used to determine a significant response: 1) tastant-solution consumption; 2) water (tasteless) consumption; 3) total consumption (tastant and water together); 4) ratio of tastant consumption to total consumption...
March 2, 2017: Poultry Science
https://www.readbyqxmd.com/read/28339766/higher-bioavailability-of-doxycycline-in-broiler-chickens-with-a-novel-in-feed-pharmaceutical-formulation
#5
Lilia Gutiérrez, Juan Zermeño, Yazmín Alcalá, Héctor Sumano
Bioavailability of a new, long-acting (LA) pharmaceutical preparation for administering doxycycline as in-feed medication to broiler chickens was compared to the standard in-feed administration of doxycycline. A commercial poultry house harboring Ross-308 broiler chickens, weighing 450 g, was divided into 7 sections as follows: doxy-FOLA group (n = 6,000 chickens divided into 3 replicates) medicated with 10% doxycyline, long-acting pellets at a dose of 400 g of doxycycline HCl/ton of food, resulting in a calculated dose of 48 mg/kg for 5 d; doxy-ref group (n = 6,000, divided into 3 replicates) medicated as for doxy-FOLA, but using a 20% commercial preparation of doxycycline...
February 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28338499/development-of-the-irritable-bowel-syndrome-cognitive-affective-scale-a-brief-self-report-measure-for-clinical-and-research-settings
#6
Sarah Ballou, Laurie Keefer
OBJECTIVES: Measures of cognitive-affective processes believed to underlie Irritable Bowel Syndrome (IBS) symptom expression are used widely in both clinical and research settings. When combined, these measures can be time-consuming and it is not clear whether they evaluate distinct or overlapping constructs. With this study, we seek to identify the most critical cognitive-affective components contributing toward the expression of IBS and to identify which items in these constructs might be streamlined into a single, brief self-report measure...
March 23, 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28338358/a-mismatch-between-athlete-practice-and-current-sports-nutrition-guidelines-among-elite-female-and-male-middle-and-long-distance-athletes
#7
Ida Aliisa Heikura, Trent Stellingwerff, Antti A Mero, Arja Leena Tuulia Uusitalo, Louise Mary Burke
Contemporary nutrition guidelines promote a variety of periodized and time-sensitive recommendations, but current information regarding the knowledge and practice of these strategies among world-class athletes is limited. The aim of this study was to investigate this theme by implementing a questionnaire on dietary periodization practices in national/international level female (n=27) and male (n=21) middle- and long-distance runners/race-walkers. The questionnaire aimed to gain information on between and within-day dietary choices, as well as timing of pre- and post-training meals and practices of training with low or high carbohydrate (CHO) availability...
March 24, 2017: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/28337267/exploration-of-the-effect-of-probiotics-supplementation-on-intestinal-microbiota-of-food-allergic-mice
#8
Bo Yang, Liang Xiao, Sheng Liu, Xiaoyu Liu, Yan Luo, Qiongmei Ji, Pingchang Yang, Zhigang Liu
Environmental factor-induced alterations in intestinal microbiota have been demonstrated to be associated with increasing prevalence of food allergy. However, it is not clear to what extent oral administration of probiotics can affect gut microbiota composition, thus inhibiting food allergy development. Using ovalbumin (OVA)-sensitized murine model, it was demonstrated that probiotics ameliorated allergic symptoms, including reducing OVA specific-IgE, and -IgG1 levels in the serum, Th2 cytokines release in spleen, and occurrence of diarrhea...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337186/genotypes-associated-with-listeria-monocytogenes-isolates-displaying-impaired-or-enhanced-tolerances-to-cold-salt-acid-or-desiccation-stress
#9
Patricia Hingston, Jessica Chen, Bhavjinder K Dhillon, Chad Laing, Claire Bertelli, Victor Gannon, Taurai Tasara, Kevin Allen, Fiona S L Brinkman, Lisbeth Truelstrup Hansen, Siyun Wang
The human pathogen Listeria monocytogenes is a large concern in the food industry where its continuous detection in food products has caused a string of recalls in North America and Europe. Most recognized for its ability to grow in foods during refrigerated storage, L. monocytogenes can also tolerate several other food-related stresses with some strains possessing higher levels of tolerances than others. The objective of this study was to use a combination of phenotypic analyses and whole genome sequencing to elucidate potential relationships between L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28337174/regulation-of-mammalian-physiology-by-interconnected-circadian-and-feeding-rhythms
#10
REVIEW
Florian Atger, Daniel Mauvoisin, Benjamin Weger, Cédric Gobet, Frédéric Gachon
Circadian clocks are endogenous timekeeping systems that adapt in an anticipatory fashion the physiology and behavior of most living organisms. In mammals, the master pacemaker resides in the suprachiasmatic nucleus and entrains peripheral clocks using a wide range of signals that differentially schedule physiology and gene expression in a tissue-specific manner. The peripheral clocks, such as those found in the liver, are particularly sensitive to rhythmic external cues like feeding behavior, which modulate the phase and amplitude of rhythmic gene expression...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28336061/enzymes-as-sensors
#11
Maria Staiano, Angela Pennacchio, Antonio Varriale, Alessandro Capo, Adelia Majoli, Clotilde Capacchione, Sabato D'Auria
Over the last few decades the development of new technologies, the fabrication of new materials, and the introduction of nanotechnologies created new trends in a series of advances that produced innovations in biological sensing devices with a wide range of application from health, security, defense, food, and medicine, to the environment. Specificity, low cost, rapidity, sensitivity, and multiplicity are some of the reasons for their growth, and their commercial success is expected to increase in the next future...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28335964/highly-sensitive-colorimetric-immunosensor-for-influenza-virus-h5n1-based-on-enzyme-encapsulated-liposome
#12
Cuiying Lin, Yajuan Guo, Mengmeng Zhao, Mi Sun, Fang Luo, Longhua Guo, Bin Qiu, Zhenyu Lin, Guonan Chen
Development of simple but sensitive biosensor for influenza detection is highly important in immediate and effective clinical treatment. In this study, a sensitive colorimetric immunosensor which combines the advantages of high selectivity of immunoassay and simplicity of colorimetric detection has been developed to detect influenza virus H5N1 based on enzyme-encapsulated liposome. Biotin-tagged liposome encapsulated with large amount of horseradish peroxidase (HRP) was firstly synthesized. In the presence of H5N1, H5N1 co-bound with the capture antibody and the biotinylated detection antibody to form sandwich immunocomplex...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28335573/ultratrace-detection-of-histamine-using-a-molecularly-imprinted-polymer-based-voltammetric-sensor
#13
Maedeh Akhoundian, Axel Rüter, Sudhirkumar Shinde
Rapid and cost-effective analysis of histamine, in food, environmental, and diagnostics research has been of interest recently. However, for certain applications, the already-existing biological receptor-based sensing methods have usage limits in terms of stability and costs. As a result, robust and cost-effective imprinted polymeric receptors can be the best alternative. In the present work, molecularly-imprinted polymers (MIPs) for histamine were synthesized using methacrylic acid in chloroform and acetonitrile as two different porogens...
March 21, 2017: Sensors
https://www.readbyqxmd.com/read/28335563/the-effects-of-sweet-foods-on-the-pharmacokinetics-of-glycyrrhizic-acid-by-icelisa
#14
Bingqian Jiang, Huihua Qu, Hui Kong, Yue Zhang, Shuchen Liu, Jinjun Cheng, Xin Yan, Yan Zhao
The effect of sweet foods, such as honey, was investigated from the perspective of pharmacokinetics on the absorption of glycyrrhizic acid (GA). Due to the unique properties of indirect competitive enzyme-linked immunosorbent assay (icELISA), namely, its: specificity, sensitivity, repeatability, simple pretreatment of samples, fast and simple operation, and because it is economic and non-polluting, it has received increased attention. In this study, we used the advantages of this method to see how honey affected the pharmacokinetics of GA...
March 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28335318/a-label-free-and-sensitive-fluorescent-qualitative-assay-for-bisphenol-a-based-on-rolling-circle-amplification-exonuclease-iii-combined-cascade-amplification
#15
Xia Li, Juan Song, Qing-Wang Xue, Fu-Heng You, Xia Lu, Yan-Cong Kong, Shu-Yi Ma, Wei Jiang, Chen-Zhong Li
Bisphenol A (BPA) detection in drinking water and food packaging materials has attracted much attention since the discovery that BPA can interfere with normal physiological processes and cause adverse health effects. Here, we constructed a label-free aptamer fluorescent assay for selective and sensitive detection of BPA based on the rolling circle amplification (RCA)/Exonuclease III (Exo III)-combined cascade amplification strategy. First, the duplex DNA probe (RP) with anti-BPA aptamer and trigger sequence was designed for BPA recognition and signal amplification...
October 21, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334650/ultrasensitive-determination-of-dna-oxidation-products-by-gas-chromatography-tandem-mass-spectrometry-and-the-role-of-antioxidants-in-the-prevention-of-oxidative-damage
#16
Sam Dawbaa, Önder Aybastıer, Cevdet Demir
Oxidative stress is considered as one of the significant causes of DNA damage which in turn contributes to cell death through a series of intermediate processes such as cancer formation, mutation, and aging. Natural sources such as plant and fruit products have provided us with interesting substances of antioxidant activity that could be recruited in protecting the genetic materials of the cells. This study is an effort to discover some of those antioxidants effects in their standard and natural forms by performing an ultrasensitive determination of the products of DNA oxidation using GC-MS/MS...
March 16, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28334404/calcium-homeostasis-modulator-1-like-currents-in-rat-fungiform-taste-cells-expressing-amiloride-sensitive-sodium-currents
#17
Albertino Bigiani
Salt reception by taste cells is still the less understood transduction process occurring in taste buds, the peripheral sensory organs for the detection of food chemicals. Although there is evidence suggesting that the epithelial sodium channel (ENaC) works as sodium receptor, yet it is not clear how salt-detecting cells signal the relevant information to nerve endings. Taste cells responding to sweet, bitter, and umami substances release ATP as neurotransmitter through a nonvesicular mechanism. Three different channel proteins have been proposed as conduit for ATP secretion: pannexin channels, connexin hemichannels, and calcium homeostasis modulator 1 (CALHM1) channels...
March 10, 2017: Chemical Senses
https://www.readbyqxmd.com/read/28334322/evaluation-of-food-lures-for-capture-and-monitoring-of-anastrepha-fraterculus-diptera-tephritidae-on-temperate-fruit-trees
#18
J M da Rosa, C J Arioli, J P Dos Santos, A C Menezes-Netto, M Botton
The Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) is the main pest of fruit trees grown in temperate climates in the southern region of Brazil. The objective of this work was to evaluate the efficiency of the major commercial food lures used in Brazil for trapping and monitoring of A. fraterculus in plum, pear, and feijoa orchards. The assessed lures were hydrolyzed proteins of animal origin (CeraTrap) and plant origin (BioAnastrepha), torula yeast + borax (Torula), and grape juice. Response variables included the rate of adult capture (flies per trap per day, FTD) and the percentage of females captured...
March 16, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28334183/comparative-transcriptional-analysis-of-the-host-specialized-aphids-aphis-gossypii-hemiptera-aphididae
#19
Yuan-Chen Zhang, Hai-Xia Lei, Ning-Hui Miao, Xiang-Dong Liu
Host specialization is an ubiquitous character in aphid populations. Many polyphagous aphid populations usually consist of several subpopulations that have strong fidelity to a specific host or a subset of host range. Host specialization is an evolutional result of food habit of insects. However, genetic basis and molecular mechanism of host specialization are still unclear. In this study, we presented a comparative analysis on global gene expression profiles of three lineages of Aphis gossypii Glover: cotton-specialized (CO), cucurbit-specialized (CU), and CU reared on cowpea (CU-cowpea), using RNA-Seq method...
February 17, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28333108/the-biology-of-pichia-membranifaciens-killer-toxins
#20
REVIEW
Ignacio Belda, Javier Ruiz, Alejandro Alonso, Domingo Marquina, Antonio Santos
The killer phenomenon is defined as the ability of some yeast to secrete toxins that are lethal to other sensitive yeasts and filamentous fungi. Since the discovery of strains of Saccharomyces cerevisiae capable of secreting killer toxins, much information has been gained regarding killer toxins and this fact has substantially contributed knowledge on fundamental aspects of cell biology and yeast genetics. The killer phenomenon has been studied in Pichia membranifaciens for several years, during which two toxins have been described...
March 23, 2017: Toxins
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