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https://www.readbyqxmd.com/read/28735045/multiplexed-capillary-microfluidic-immunoassay-with-smartphone-data-acquisition-for-parallel-mycotoxin-detection
#1
Jessica M D Machado, Ruben R G Soares, Virginia Chu, João P Conde
The field of microfluidics holds great promise for the development of simple and portable lab-on-a-chip systems. The use of capillarity as a means of fluidic manipulation in lab-on-a-chip systems can potentially reduce the complexity of the instrumentation and allow the development of user-friendly devices for point-of-need analyses. In this work, a PDMS microchannel-based, colorimetric, autonomous capillary chip provides a multiplexed and semi-quantitative immunodetection assay. Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure...
July 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28734358/fluorescent-quenching-immune-chromatographic-strips-with-quantum-dots-and-upconversion-nanoparticles-as-fluorescent-donors-for-visual-detection-of-sulfaquinoxaline-in-foods-of-animal-origin
#2
Gaoshuang Hu, Wei Sheng, Jingmin Li, Yan Zhang, Junping Wang, Shuo Wang
In this study, two novel fluorescence quenching immune chromatographic strips (FQICS) were developed to detect sulfaquinoxaline (SQX) in foods of animal origin. These proposed FQICSs were based on fluorescence resonance energy transfer (FRET) from fluorescence donors (quantum dots or upconversion nanoparticles) to fluorescence acceptors (colloidal gold nanoparticles). Compared with traditional colloidal gold-based immune chromatographic strips (ICS), these FQICSs showed positive correlation between the fluorescent signals and the targets, and allowed user to get test results from weak fluorescent signals...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28734214/effects-of-damming-on-the-distribution-and-methylation-of-mercury-in-wujiang-river-southwest-china
#3
Lei Zhao, Yanna Guo, Bo Meng, Heng Yao, Xinbin Feng
Newly built reservoirs are regarded as sensitive ecosystem for mercury (Hg) methylation. A comprehensive study was conducted to understand the influence of damming on the distribution and methylation of Hg within a river-reservoir ecosystem in Wujiang River Basin (WRB), Southwest China. Hg species in inflow-outflow rivers of six cascade reservoirs were analyzed each month during 2006. Mean concentrations of total Hg (THg) and methylmercury (MeHg) in river water in WRB were 3.41 ± 1.98 ng L(-1) and 0.15 ± 0...
July 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28731389/protease-hydrolysates-of-filefish-thamnaconus-modestus-byproducts-effectively-inhibit-foodborne-pathogens
#4
Shaimaa Hatab, Mei-Ling Chen, Wenhua Miao, Jiheng Lin, Dandan Wu, Changyu Wang, Pengxiang Yuan, Shanggui Deng
In this study, novel antimicrobial peptides had been derived by enzymatic hydrolysis of filefish (Thamnaconus modestus) byproduct (HFBP). Different proteases, (papain [P], trypsin [T], neutrase [N], pepsin [PE], and the mixture I [PN] and mixture II [PT]) and different hydrolysis time (60, 120, 180, and 240 min), have been used to generate peptides with different lengths and amino acid sequences. The antimicrobial properties of HFBPs were tested, against Gram-positive and Gram-negative strains, using disc diffusion method...
July 21, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28731314/desensitization-to-chemical-and-food-sensitivities-by-low-dose-immunotherapy-ascertained-by-provocation-neutralization-is-associated-with-reduced-influx-of-calcium-ions-into-lymphocytes
#5
Basant K Puri, John McLaren Howard, Jean A Monro
Background Food and chemical sensitivities have detrimental effects on health and the quality of life. The natural course of such sensitivities can potentially be altered through various types of allergen-specific immunotherapy, including low-dose immunotherapy. The molecular mechanism by which low-dose immunotherapy causes desensitization has not thus far been elucidated. While resting lymphocytes maintain a low cytosolic calcium ion concentration, antigen receptor signaling results in calcium ion influx, predominantly via store-operated calcium channels...
April 26, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28730728/beneficial-long-term-antidiabetic-actions-of-n-and-c-terminally-modified-analogues-of-apelin-13-in-diet-induced-obese-diabetic-mice
#6
V Parthsarathy, C Hogg, P R Flatt, F P M O'Harte
AIMS: This study investigated the chronic effects of twice daily administration of stable apelin analogues, apelin-13 amide and (pGlu)apelin-13 amide, on metabolic parameters in glucose intolerant and insulin resistant diet-induced obese (DIO) mice fed a high-fat diet for 150 days. STUDY DESIGN & METHODS: Groups of mice received twice daily (09:00 and 17:00 h) injections of saline vehicle, apelin-13 amide, (pGlu)apelin-13 amide or exendin-4(1-39) for 28 days (all at 25 nmol/kg)...
July 20, 2017: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/28730638/plasma-pharmacokinetics-of-quinocetone-in-ducks-after-oral-and-intravenous-administration
#7
M Qiu, Z Hao, R Zhang, L Cui, C Wang, S Qu, S Yuan, Y Bai
Quinocetone (QCT), an antimicrobial growth promoter, is widely used in food-producing animals. However, information about pharmacokinetics (PK) of QCT in ducks still remains unavailable up to now. In this study, QCT and its major metabolites (1-desoxyquinocetone, di-desoxyquinocetone and 3-methyl-quinoxaline-2-carboxylic) in ducks were studied using a simple and sensitive UHPLC-MS/MS assay. Twenty ducks were divided into two groups. (n = 10/group). One group received QCT by oral administration at dose of 40 mg/kg while another group received QCT intravenously at 10 mg/kg...
July 20, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28729703/the-basal-free-fatty-acid-concentration-in-human-saliva-is-related-to-salivary-lipolytic-activity
#8
Eric Neyraud, Stéphanie Cabaret, Hélène Brignot, Claire Chabanet, Hélène Labouré, Elisabeth Guichard, Olivier Berdeaux
Fat perception during eating is a complex sensation that involves various sensory modalities, such as texture, aroma and taste. Taste is supported by the discovery of fatty acid receptors in the tongue papillae. Dietary fat is mainly composed of esterified fatty acids, whereas only free fatty acids can bind to taste receptors. Some authors have mentioned the necessity and efficiency of salivary lipolytic activity to hydrolyse the esterified fatty acids present in foods and enable fat perception. Our hypothesis is that salivary lipolytic activity is also involved in regulating the basal level of salivary fatty acids in humans...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28726767/fat-taste-sensitivity-is-associated-with-short-term-and-habitual-fat-intake
#9
Andrew Costanzo, Liliana Orellana, Caryl Nowson, Konsta Duesing, Russell Keast
Evidence suggests individuals less sensitive to fat taste (high fat taste thresholds (FTT)) may be overweight or obese and consume greater amounts of dietary fat than more sensitive individuals. The aims of this study were to assess associations between FTT, anthropometric measurements, fat intake, and liking of fatty foods. FTT was assessed in 69 Australian females (mean age 41.3 (15.6) (SD) years and mean body mass index 26.3 (5.7) kg/m²) by a 3-alternate forced choice methodology and transformed to an ordinal scale (FT rank)...
July 20, 2017: Nutrients
https://www.readbyqxmd.com/read/28726509/soymilk-fermentation-by-enterococcus-faecalis-vb43-leads-to-reduction-in-the-immunoreactivity-of-allergenic-proteins-%C3%AE-conglycinin-7s-and-glycinin-11s
#10
V Biscola, A Rodriguez de Olmos, Y Choiset, H Rabesona, M S Garro, F Mozzi, J-M Chobert, M Drouet, T Haertlé, B D G M Franco
Food allergies represent a serious problem affecting human health and soy proteins rank among the most allergenic proteins from food origin. The proteolytic enzymes produced by lactic acid bacteria (LAB) can hydrolyse the major allergens present in soybean, reducing their immunoreactivity. Many studies have reported the ability of LAB to ferment soy-based products; while the majority of them focus on the improvement of the sensory characteristics and functionality of soy proteins, a lack of information about the role of lactic fermentation in the reduction of immunoreactivity of these proteins exists...
July 20, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28725335/is-peanut-causing-food-allergy-in-cuba-preliminary-assessment-of-allergic-sensitization-and-ige-specificity-profile-to-peanut-allergens-in-cuban-allergic-patients
#11
Mayteé Mateo-Morejón, Alexis Labrada-Rosado, Damaris Torralba-Averoff, Rayza Cruz-Jimenez, Yunia Oliva-Díaz, Mirta Álvarez-Castelló, Alexander Ciria-Martín, Marlene Jiménez-Frandín, Mary Carmen Reyes-Zamora, Raúl Lázaro Castro-Almarales, Beatriz Tamargo-García
BACKGROUND: Peanut allergy is increasing at an alarming pace in developed countries. Peanut (Arachis hypogaea) is a common food in Cuba. Nevertheless, reported values of sensitization and symptom severity are usually low. As our objective, we carried out an evaluation of allergic sensitivity to perform an assessment of allergic sensitization and IgE specificity profile to peanut allergens in Cuban allergic patients. METHODS: The Skin Prick Test (SPT) was performed for each patient, using two glycerinated allergenic extracts, prepared from raw or roasted peanuts...
2017: World Allergy Organization Journal
https://www.readbyqxmd.com/read/28724729/food-web-persistence-in-fragmented-landscapes
#12
Jinbao Liao, Daniel Bearup, Bernd Blasius
Habitat destruction, characterized by patch loss and fragmentation, is a key driver of biodiversity loss. There has been some progress in the theory of spatial food webs; however, to date, practically nothing is known about how patch configurational fragmentation influences multi-trophic food web dynamics. We develop a spatially extended patch-dynamic model for different food webs by linking patch connectivity with trophic-dependent dispersal (i.e. higher trophic levels displaying longer-range dispersal). Using this model, we find that species display different sensitivities to patch loss and fragmentation, depending on their trophic position and the overall food web structure...
July 26, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28724689/california-scrub-jays-reduce-visual-cues-available-to-potential-pilferers-by-matching-food-colour-to-caching-substrate
#13
Laura A Kelley, Nicola S Clayton
Some animals hide food to consume later; however, these caches are susceptible to theft by conspecifics and heterospecifics. Caching animals can use protective strategies to minimize sensory cues available to potential pilferers, such as caching in shaded areas and in quiet substrate. Background matching (where object patterning matches the visual background) is commonly seen in prey animals to reduce conspicuousness, and caching animals may also use this tactic to hide caches, for example, by hiding coloured food in a similar coloured substrate...
July 2017: Biology Letters
https://www.readbyqxmd.com/read/28724546/high-protein-diet-promotes-sensitivity-to-cholecystokinin-and-shifts-the-cecal-microbiome-without-altering-brain-inflammation-in-diet-induced-obesity-in-rats
#14
Lixin Wang, Jonathan P Jacobs, Venu Lagishetty, Pu-Qing Yuan, Shuping Vincent Wu, Mulugeta Million, Joseph R Reeve, Joseph R Pisegna, Yvette Taché
High protein diet (HPD) curtails obesity and/or fat mass but it is unknown whether it reverses neuroinflammation, altered glucose levels, cholecystokinin (CCK) sensitivity and gut microbiome in rats fed a Western diet (WD)-induced obesity (DIO). Male rats fed a WD (high fat and sugar) for 12 weeks were switched to a HPD for 6 weeks. Body composition, food intake, meal pattern, sensitivity to intraperitoneal CCK-8S, blood glucose, brain signaling, and cecal microbiota were assessed. Compared to normal diet, WD increased body weight (9...
July 19, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28723214/electrokinetic-manipulation-integrated-plasmonic-photonic-hybrid-raman-nanosensors-with-dually-enhanced-sensitivity
#15
Chao Liu, Zheng Wang, Erwen Li, Zexi Liang, Swapnajit Chakravarty, Xiaochuan Xu, Alan X Wang, Ray T Chen, Donglei Fan
To detect biochemicals with ultrahigh sensitivity, efficiency, reproducibility, and specificity has been the holy grail in the development of nanosensors. In this work, we report an innovative type of photonic-plasmonic hybrid Raman nanosensor integrated with electrokinetic manipulation by rational design, which offers dual mechanisms that enhance the sensitivity for molecule detection directly in solution. For the first time, we integrate large arrays of synthesized plasmonic nanocapsules with densely surface distributed silver (Ag) nanoparticles (NPs) on lithographically patterned photonic crystal slabs via electric-field assembling...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28723200/flexible-transparent-and-free-standing-silicon-nanowire-sers-platform-for-in-situ-food-inspection
#16
Hao Cui, Shuoyu Li, Shaozhi Deng, Huanjun Chen, Chengxin Wang
We demonstrated a flexible transparent and free-standing Si nanowire paper (SiNWP) as a surface enhanced Raman scattering (SERS) platform for in situ chemical sensing on warping surfaces with high sensitivity. The SERS activity has originated from the three-dimension interconnected nanowire network structure and electromagnetic coupling between closely separated nanowires in the SiNWP. In addition, the SERS activity can be highly improved by functionalizing the SiNWP with plasmonic Au nanoparticles. The hybrid substrate not only showed excellent reproducibility and stability of the SERS signal, but also maintained the flexibility and transparency of the pristine SiNWP...
March 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28723196/label-free-homogeneous-electroanalytical-platform-for-pesticide-detection-based-on-acetylcholinesterase-mediated-dna-conformational-switch-integrated-with-rolling-circle-amplification
#17
Xiaojuan Liu, Mengmeng Song, Ting Hou, Feng Li
This study addresses the need for sensitive pesticide assay by reporting a new label-free and immobilization-free homogeneous electroanalytical strategy, which combines acetylcholinesterase (AChE)-catalyzed hydrolysis product-mediated DNA conformational switch and rolling circle amplification (RCA) to detect organophosphorous and carbamate pesticides in a "signal-on" mode. When target pesticides were present, AChE activity was inhibited and could not trigger the following DNA conformational change and the RCA reaction, which results in numerous methylene blue (MB) molecules in a free state, generating a strong electrochemical response...
April 28, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28723147/quantum-dot-based-molecularly-imprinted-polymers-on-three-dimensional-origami-paper-microfluidic-chip-for-fluorescence-detection-of-phycocyanin
#18
Bowei Li, Zhong Zhang, Ji Qi, Na Zhou, Song Qin, Jaebum Choo, Lingxin Chen
In this work, we developed a novel strategy using fluorescent quantum dots (QDs) combined with molecularly imprinted polymers (MIPs) on three-dimensional (3D) origami paper-based microfluidic devices for specific recognition and sensitive detection of phycocyanin. This method can realize the liquid phase of QDs@MIPs being transferred to the solid-phase paper base and achieve easy portability for the analysis. Under optimal conditions, we successfully demonstrated the proposed paper@QDs@MIPs 3D microfluidic chip for the sensitive and selective detection of phycocyanin protein target in a simple and robust manner...
February 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28722482/an-economic-evaluation-of-food-safety-education-interventions-estimates-and-critical-data-gaps
#19
Hua Zan, Maria Lambea, Joyce McDowell, Robert L Scharff
The economic evaluation of food safety interventions is an important tool that practitioners and policy makers use to assess the efficacy of their efforts. These evaluations are built on models that are dependent on accurate estimation of numerous input variables. In many cases, however, there is no data available to determine input values and expert opinion is used to generate estimates. This study uses a benefit-cost analysis of the food safety component of the adult Expanded Food and Nutrition Education Program (EFNEP) in Ohio as a vehicle for demonstrating how results based on variable values that are not objectively determined may be sensitive to alternative assumptions...
July 19, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28721842/rapid-seafood-species-identification-using-chip-based-capillary-electrophoresis-and-protein-pattern-matching
#20
Calvin Walker, Cheryl Lassitter, Shannara Lynn, Courtney Ford, Kevin Rademacher, Angela Ruple, Jon Bell
Authenticity is crucial to the seafood industry, as substitution and mislabeling have important economic, environmental, and food safety consequences. To address this problem, protein profiling and software algorithm techniques were developed to classify fish muscle samples by species. The method uses water-based protein extraction, chip-based microfluidic electrophoresis (Agilent 2100 Bioanalyzer) for the analysis of high abundance fish muscle proteins, and a novel data analysis method for species-specific protein pattern recognition...
July 19, 2017: Journal of AOAC International
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