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https://www.readbyqxmd.com/read/28933248/removals-of-estrone-and-17%C3%AE-estradiol-by-microalgae-cultivation-kinetics-and-removal-mechanisms
#1
Chantima Ruksrithong, Songkeart Phattarapattamawong
Occurrence of estrone (E1) and 17β-estradiol (E2) in environment has attracted the public attention since E1 and E2 cause estrogenicity on aquatic microorganisms. Microalgae cultivation in wastewater is considered as an economical practice since microalgae can be used for not only pollutant removals, but also as energy resources. This research aims to investigate the removals of E1 and E2 in synthetic wastewater by microalgae (i.e. Chlorella vulgaris and Scenedesmus obliquus). At the steady state, Scenedesmus obliquus removed E1 and E2 by 91% and 99%, respectively...
September 21, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28929006/a-comparison-of-the-morphological-and-biochemical-characteristics-of-chlorella-sorokiniana-and-chlorella-zofingiensis-cultured-under-photoautotrophic-and-mixotrophic-conditions
#2
Siti Nor Ani Azaman, Norio Nagao, Fatimah M Yusoff, Sheau Wei Tan, Swee Keong Yeap
The responses of two species of microalgae, Chlorella sorokiniana and Chlorella zofingiensis, were compared regarding their morphological and biochemical properties under photoautotrophic and mixotrophic conditions. These microalgae were cultured under both conditions, and their crude ethanolic extracts were examined for their pigment and total phenolic contents. In addition, the microalgae's antioxidant activities were determined using a DPPH radical scavenging assay and a ferric reducing antioxidant power (FRAP) assay...
2017: PeerJ
https://www.readbyqxmd.com/read/28919927/characterization-of-a-heat-tolerant-chlorella-sp-gd-mutant-with-enhanced-photosynthetic-co2-fixation-efficiency-and-its-implication-as-lactic-acid-fermentation-feedstock
#3
Tse-Min Lee, Yu-Fei Tseng, Chieh-Lun Cheng, Yi-Chuan Chen, Chih-Sheng Lin, Hsiang-Yen Su, Te-Jin Chow, Chun-Yen Chen, Jo-Shu Chang
BACKGROUND: Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass. RESULTS: A Chlorella sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine to overcome outdoor high-temperature inhibition and it was used as a feedstock for fermentative lactic acid production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28898861/removal-and-metabolism-of-triclosan-by-three-different-microalgal-species-in-aquatic-environment
#4
Shujuan Wang, Karen Poon, Zongwei Cai
Triclosan, an antimicrobial additive widely used in personal care products, has caused the contamination of various aquatic environment. Biodegradation was proved to play a vital role in the treatment of triclosan in wastewater. However, there is limited information about the metabolic pathway. In this study, three common freshwater microalgae including Chlorella pyrenoidosa (C. pyrenoidosa), Desmodesmus sp., and Scenedesmus obliquus (S. obliquus) were applied to remove and biodegrade triclosan in aqueous culture medium...
September 5, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28898848/comparative-evaluation-of-piggery-wastewater-treatment-in-algal-bacterial-photobioreactors-under-indoor-and-outdoor-conditions
#5
Dimas García, Esther Posadas, Carlos Grajeda, Saúl Blanco, Sonia Martínez-Páramo, Gabriel Acién, Pedro García-Encina, Silvia Bolado, Raúl Muñoz
This work evaluated the performance of four open algal-bacterial photobioreactors operated at ≈26days of hydraulic retention time during the treatment of 10 (×10) and 20 (×20) times diluted piggery wastewater (PWW) under indoor (I) and outdoor (O) conditions for four months. The removal efficiencies (REs) of organic matter, nutrients and zinc from PWW, along with the dynamics of biomass concentration and structure of algal-bacterial population were assessed. The highest TOC-RE, TP-RE and Zn-RE (94±1%, 100% and 83±2%, respectively) were achieved indoors in ×10 PWW, while the highest TN-RE (72±8%) was recorded outdoors in ×10 PWW...
August 24, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28895365/-expression-of-leafy-cotyledon-2-from-arabidopsis-increased-the-content-of-lipid-in-chlorella-sorokiniana
#6
Changbin Liu, Shaoming Tong, Wenlei Zhang, Hesheng Hou
The low lipid content is one of the major bottlenecks to realize the industrialization of the algae biodiesel. Improvement of lipid content through global regulation to get high-yield generating algae is a good strategy. Leafy Cotyledon 2 (LEC2) is an important transcription factor for seed maturation and oil accumulation in Arabidopsis. However, there are few reports regarding adoption of LEC2 for lipid accumulation until now. In this study, LEC2 from Arabidopsis was cloned into the plant expression vector pCIMBIA1300 and transformed into C...
June 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28892701/co-cultivation-of-microalgae-in-aquaponic-systems
#7
Min M Addy, Faryal Kabir, Renchuan Zhang, Qian Lu, Xiangyuan Deng, Dean Current, Richard Griffith, Yiwei Ma, Wenguang Zhou, Paul Chen, Roger Ruan
Aquaponics is a sustainable system for the future farming. In aquaponic systems, the nutrient-rich wastewater generated by the fish provides nutrients needed for vegetable growth. In the present study, the role of microalgae of Chlorella sp. in the floating-raft aquaponic system was evaluated for ammonia control. The yields of algal biomass, vegetable, and removal of the key nutrients from the systems were monitored during the operation of the aquaponic systems. When the systems were in full operation, the algae production was about 4...
August 25, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28892686/autotrophic-and-heterotrophic-microalgae-and-cyanobacteria-cultivation-for-food-and-feed-life-cycle-assessment
#8
Sergiy Smetana, Michael Sandmann, Sascha Rohn, Daniel Pleissner, Volker Heinz
The lack of protein sources in Europe could be reduced with onsite production of microalgae with autotrophic and heterotrophic systems, owing the confirmation of economic and environmental benefits. This study aimed at the life cycle assessment (LCA) of microalgae and cyanobacteria cultivation (Chlorella vulgaris and Arthrospira platensis) in autotrophic and heterotrophic conditions on a pilot industrial scale (in model conditions of Berlin, Germany) with further biomass processing for food and feed products...
August 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28881198/ecophysiological-strategies-for-growth-under-varying-light-and-organic-carbon-supply-in-two-species-of-green-microalgae-differing-in-their-motility
#9
Elly Spijkerman, Marcus Lukas, Alexander Wacker
Mixing events in stratified lakes result in microalgae being exposed to varying conditions in light and organic carbon concentrations. Stratified lakes consist of an upper illuminated strata and a lower, darker strata where organic carbon accumulates. Therefore, in this contribution we explore the importance of dissolved organic carbon for growth under various light intensities by measuring some ecophysiological adaptations in two green microalgae. We compared the non-motile Chlorella vulgaris with the flagellated Chlamydomonas acidophila under auto-, mixo-, and heterotrophic growth conditions...
September 4, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28876029/-bioassay-guided-isolation-of-functional-components-from-hot-water-extract-of-chlorella-pyrenoidosa
#10
Jing Jia, Diansheng Xu, Xiuyuan Zhuang, Daojing Zhang, Liming Tao, Yuanguang Li
The main functional ingredients of hot water extract of Chlorella pyrenoidosa (CPE) were investigated through a bioassay-guided fractionation based on free radical scavenging and macrophage proliferation effects. The main functional ingredients of CPE were polysaccharides (PS) that were isolated by high pressure extraction, Sevag method, ethanol precipitation and ultrafiltration separation. Crude polysaccharides were further separated and purified by ion exchange chromatography DEAE52 and size exclusion chromatography Sephadex G-100...
May 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28873686/carotenoid-profile-of-three-microalgae-cyanobacteria-species-with-peroxyl-radical-scavenger-capacity
#11
Luciana D Patias, Andrêssa S Fernandes, Fabiane C Petry, Adriana Z Mercadante, Eduardo Jacob-Lopes, Leila Q Zepka
Carotenoids from cyanobacteria Aphanothece microscopica Nageli and green microalgae Chlorella vulgaris and Scenedesmus obliquus were identified. The total carotenoid content, based on dry weight of biomass, of A. microscopica Nägeli, C. vulgaris and S. obliquus were 1398.88μg/g, 1977.02μg/g and 2650.70μg/g, respectively. A total of 23 different carotenoids were separated in all the extracts, the major ones being all-trans-β-carotene (29.3%) and all-trans-lutein (28.1%) in Scenedesmus; all-trans-echinenone (22...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28871544/calorespirometry-a-novel-tool-in-functional-hologenomics-to-select-green-holobionts-for-biomass-production
#12
Birgit Arnholdt-Schmitt, Vinod Kumar Patil
Endophytes can diversify temperature response and biomass production in plants and microalgae. Natural and inoculated endophytes that modify growth performance are increasingly considered in research and practical initiatives for sustainable agriculture. However, efficient, novel tools are required that are able to support identification of differential effects of native endophyte populations and for pre-selection of inocula.This protocol gives instructions for applying calorespirometry as a rapid means for identifying differential effects of endophytes on temperature response and predicted biomass productivity in microalgae and plant holobionts...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28871522/the-acute-toxicity-of-bisphenol-a-and-lignin-derived-bisphenol-in-algae-daphnids-and-japanese-medaka
#13
Dan Li, Ran Bi, Hongxing Chen, Lei Mu, Lina Zhang, Qin Chen, Haibo Xie, Yongju Luo, Lingtian Xie
Risk assessing newly synthesized chemicals prior to their applications is extremely important, if we want to ensure substitution of risky chemicals with more benign ones. During the past two decades, many analogs of bisphenol A (BPA) have been manufactured, while their toxicity remains less studied. The aim of this study was to compare the acute toxicity of a synthesized lignin-derived BPA (LD-BP) with that of BPA in representative aquatic organisms including two algal species (Chlorella pyrenoidosa and Scenedesmus obliquus), a cladoceran species (Daphnia magna), and the Japanese medaka (Oryzias latipes)...
September 4, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28871394/the-effects-of-hydraulic-retention-time-hrt-on-chromium-vi-reduction-using-autotrophic-cultivation-of-chlorella-vulgaris
#14
Ling Lee, Chih-Yuan Hsu, Hong-Wei Yen
Chromium is an acutely toxic heavy metal that is known to be a carcinogen. Of the two predominant forms of chromium, Cr(III) and Cr(VI), Cr(III) has only about one thousandth the toxicity of Cr(VI). Using microalgal biomass is one way to remove Cr(VI) from the environment. Four days of hydraulic retention time (HRT) was required to completely reduce 10 mg/L of Cr(VI) in the influent. Microalgal biomass is conventionally regarded as an adsorbent in most Cr(VI) reduction studies. However, this study found that Chlorella vulgaris had the potential to convert Cr(VI) to Cr(III) through the enzymatic route of chromium reductase although the measured chromium reductase activity of C...
September 4, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28870010/optimal-temperature-and-light-intensity-for-improved-mixotrophic-metabolism-of-chlorella-sorokiniana-treating-livestock-wastewater
#15
Tae-Hun Lee, Jae-Kyung Jang, Hyun-Woo Kim
Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experiments using a mixotroph, Chlorella sorokiniana, to characterize optimal guidelines of mixotrophic growth based on the statistical design of experiment. Using a central composite design, this study evaluated how temperature and light irradiance is associated with the CO₂ capture and organic carbon respiration through biomass production and ammonia removal kinetics...
September 5, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28869730/-effect-of-sodium-chloride-on-growth-and-lipid-accumulation-of-chlorella-protothecoides-cs-41
#16
Jieli Pan, Li'na Gao, Shuangshuang Zhao, Qiao Liu, Jifeng Yu, Yijun He, Meiya Li
With basal medium, we studied the growth status, lipid droplet distribution, total lipid content of Chlorella protothecoides CS-41 treated with different concentrations of sodium chloride (0, 150, 300 and 600 mmol/L) by optical microscopy, electron microscopy, confocal laser focusing and Nile red staining. Results show that the addition of NaCl affected the growth of Chlorella protothecoides CS-41. With the increase of NaCl concentration, the growth rate of Chlorella was inhibited. Chlorella cell wall became thicker, and lipid droplets increased...
July 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28860619/microcystis-aeruginosa-strengthens-the-advantage-of-daphnia-similoides-in-competition-with-moina-micrura
#17
Hengxing Tang, Xinying Hou, Xiaofeng Xue, Rui Chen, Xuexia Zhu, Yuan Huang, Yafen Chen
Microcystis blooms are generally associated with zooplankton shifts by disturbing interspecific relationships. The influence of Microcystis on competitive dominance by different sized zooplanktons showed species-specific dependence. We evaluated the competitive responses of small Moina micrura and large Daphnia similoides to the presence of Microcystis using mixed diets comprising 0%, 20%, and 35% of toxic M. aeruginosa, and the rest of green alga Chlorella pyrenoidosa. No competitive exclusion occurred for the two species under the tested diet combinations...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28860382/an-algal-photoenzyme-converts-fatty-acids-to-hydrocarbons
#18
Damien Sorigué, Bertrand Légeret, Stéphan Cuiné, Stéphanie Blangy, Solène Moulin, Emmanuelle Billon, Pierre Richaud, Sabine Brugière, Yohann Couté, Didier Nurizzo, Pavel Müller, Klaus Brettel, David Pignol, Pascal Arnoux, Yonghua Li-Beisson, Gilles Peltier, Fred Beisson
Although many organisms capture or respond to sunlight, few enzymes are known to be driven by light. Among these are DNA photolyases and the photosynthetic reaction centers. Here, we show that the microalga Chlorella variabilis NC64A harbors a photoenzyme that acts in lipid metabolism. This enzyme belongs to an algae-specific clade of the glucose-methanol-choline oxidoreductase family and catalyzes the decarboxylation of free fatty acids to n-alkanes or -alkenes in response to blue light. Crystal structure of the protein reveals a fatty acid-binding site in a hydrophobic tunnel leading to the light-capturing flavin adenine dinucleotide (FAD) cofactor...
September 1, 2017: Science
https://www.readbyqxmd.com/read/28860062/effects-of-the-molecular-weight-and-protein-and-sulfate-content-of-chlorella-ellipsoidea-polysaccharides-on-their-immunomodulatory-activity
#19
Jia Qi, Sang Moo Kim
We investigated the effects of the protein and sulfate content, as well as the molecular weight (Mw), of green alga Chlorella ellipsoidea polysaccharides on their immunomodulatory activity. The deproteinized (DP1-3), desulfated (DS1-3), and hydrolyzed (DH1-3) derivatives of C. ellipsoidea polysaccharides were prepared by enzymatic hydrolysis, desulfation, and acid hydrolysis, respectively, of differing durations, resulting in preparations containing various amounts of proteins (2.41%-8.97%), sulfates (1.36%-4...
August 28, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28858716/assessment-of-toxic-effects-of-magnetic-particles-used-for-lake-restoration-on-chlorella-sp-and-on-brachionus-calyciflorus
#20
I Álvarez-Manzaneda, I de Vicente
Laboratory tests, by following standardized Organization for Economic Co-operation and Development (OECD) protocols, were run for evaluating the acute effects of iron magnetic microparticles (MPs), recently proposed for lake restoration, on Chlorella sp. (algal growth) and on the rotifer B. calyciflorus (mortality). In addition, the MPs potential indirect effects on rotifer egg bank were assessed by performing hatching rate test with B. calyciflorus cysts in contact with dissolved iron (Tot-Fedis). In the algal growth test, no inhibition occurred at the two lowest MPs concentrations (0...
August 25, 2017: Chemosphere
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