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https://www.readbyqxmd.com/read/29793118/efficient-resource-recycling-from-liquid-digestate-by-microalgae-yeast-mixed-culture-and-the-assessment-of-key-gene-transcription-related-to-nitrogen-assimilation-in-microalgae
#1
Lei Qin, Lu Liu, Zhongming Wang, Weining Chen, Dong Wei
To determine the feasibility of microalgae-yeast mixed culture using the liquid digestate of dairy wastewater (LDDW) for biofuels and single cell protein (SCP) production, the cell growth, nutrient removal and outputs evaluation of the mono and mixed culture of Chlorella vulgaris and Yarrowia lipolytica in LDDW were investigated by adding glycerol as carbon source. The results showed that the mixed culture could enhance the biological utilization efficiency of nitrogen and phosphorus, and obtain higher yield of biomass (1...
May 18, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29780062/effect-of-chlorella-ingestion-on-oxidative-stress-and-fatigue-symptoms-in-healthy-men
#2
Hirotaka Okada, Noriko Yoshida, Tatsuyuki Kakuma, Kouji Toyomasu
OBJECTIVE: We examined the effects of dietary chlorella ingestion on oxidative stress and fatigue symptoms in healthy men under resting and fatigue conditions. METHOD: We conducted a double-blind, parallel-arm controlled study. Twenty-seven healthy male volunteers (mean age, 35.4±10.4 years) were randomly divided into the chlorella and placebo groups, and received chlorella (6 g/day) and lactose as placebo (7.2 g/day), respectively, for 4 weeks. To simulate mild fatigue, subjects underwent exercise (40% of the heart rate reserve) for 30 minutes...
May 21, 2018: Kurume Medical Journal
https://www.readbyqxmd.com/read/29779121/a-comprehensive-comparable-study-of-the-physiological-properties-of-four-microalgal-species-under-different-light-wavelength-conditions
#3
Yu Zhong, Peng Jin, Jay J Cheng
Microalgae treated with blue light have potential for production of human nutrition supplement and biofuel due to their higher biomass productivity and favorable fatty acid composition. Chlorella vulgaris, Chlorella pyrenoidosa, Scenedesmus quadricauda and Scenedesmus obliquus are representative green microalgae which are widely reported for algal production. In this study, we provide a systematic investigation of the biomass productivity, photosynthetic pigments, chlorophyll fluorescence and fatty acid content of the four green microalgae...
May 19, 2018: Planta
https://www.readbyqxmd.com/read/29768724/on-the-simultaneous-identification-and-quantification-of-microalgae-populations-based-on-fluorometric-techniques
#4
Natalia S Gsponer, M Claudia Rodríguez, Rodrigo E Palacios, Carlos A Chesta
In this study, the phytoplankton structure of a freshwater reservoir located in central Argentina (Embalse Río Tercero) was analyzed using Beutler's method (Photosynthesis Research 72: 39-53, 2002), aiming to provide water quality control agencies with a reliable tool for early detection of algae blooms, particularly cyanobacteria. The method estimates the concentration of chlorophyll a (Chl a) contributed by individual algal groups in a real sample by fitting its fluorescence excitation spectrum to a linear combination of norm spectra of relevant algae groups...
May 16, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29764765/easy-reuse-of-magnetic-cross-linked-enzyme-aggregates-of-lipase-b-from-candida-antarctica-to-obtain-biodiesel-from-chlorella-vulgaris-lipids
#5
Enrique Angulo Picó, Carmen López, Álvaro Cruz-Izquierdo, Mercedes Munarriz, Francisco Javier Iruretagoyena, Juan Luis Serra, María Jesús Llama
In this work, magnetic cross-linked enzyme aggregates (mCLEAs) of CALB (lipase B from Candida antarctica) were prepared and characterized. Moreover, a method for an easy, sustainable and economic extraction of lipids from nitrogen-starved cells of Chlorella vulgaris var L3 was developed. Then, the extracted lipids (oils and free fatty acids, FFAs) were converted to biodiesel using mCLEAs and chemical acid catalysis. Among several lipid extraction methods, saponification was selected given the amount of wet microalgal biomass it can process per unit of time, its low market value, and because it allows for the use of less toxic solvents...
May 12, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29763703/influence-of-nitrogen-on-growth-biomass-composition-production-and-properties-of-polyhydroxyalkanoates-phas-by-microalgae
#6
Samantha Serra Costa, Andréa Lobo Miranda, Bianca Bomfim Andrade, Denilson de Jesus Assis, Carolina Oliveira Souza, Michele Greque de Morais, Jorge Alberto Vieira Costa, Janice Izabel Druzian
This study sought to evaluate influence of nitrogen availability on cell growth, biomass composition, production, and the properties of polyhydroxyalkanoates during cultivation of microalgae Chlorella minutissima, Synechococcus subsalsus, and Spirulina sp. LEB-18. The cellular growth of microalgae reduced with the use of limited nitrogen medium, demonstrating that nitrogen deficiency interferes with the metabolism of microorganisms and the production of biomass. The biochemical composition of microalgae was also altered, which was most notable in the degradation of proteins and chlorophylls and the accumulation of carbonaceous storage molecules such as lipids and polyhydroxyalkanoates...
May 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29750518/altered-microbiome-leads-to-significant-phenotypic-and-transcriptomic-differences-in-a-lipid-accumulating-chlorophyte
#7
Lubna Richter, Cresten B Mansfeldt, Michael M Kuan, Alexandra Cesare, Stephen T Menefee, Ruth E Richardson, Beth A Ahner
Given the challenges facing the economically favorable production of products from microalgae, understanding factors that might impact productivity rates including growth rates and accumulation of desired products, e.g., triacylglycerols (TAG) for biodiesel feedstock, remains critical. Although operational parameters such as media composition and reactor design can clearly effect growth rates, the role of microbe-microbe interactions is just beginning to be elucidated. In this study an oleaginous marine algae Chlorella spp...
May 11, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29748798/an-innovative-approach-to-attached-cultivation-of-chlorella-vulgaris-using-different-materials
#8
Negar Jafari, Razieh Shafiee Alavijeh, Ali Abdolahnejad, Hossein Farrokhzadeh, Mohammad Mehdi Amin, Afshin Ebrahimi
This article investigates the innovative attached cultivation of Chlorella vulgaris (C. vulgaris) using different materials as an alternative to high capital techniques of harvesting such as centrifugation, flocculation, and filtration. A simple attached algal cultivation system was proposed that was equipped by 10 submerged supporting materials which can harvest algal cells, efficiently. The effect of operational parameters such as light intensity, the rate of aeration, and auto-harvesting time was investigated...
May 10, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29747629/in-vitro-evaluation-of-the-synergistic-antioxidant-and-anti-inflammatory-activities-of-the-combined-extracts-from-malaysian-ganoderma-lucidum-and-egyptian-chlorella-vulgaris
#9
Marwa M Abu-Serie, Noha H Habashy, Wafaa E Attia
BACKGROUND: Since oxidative stress and inflammation are two linked factors in the pathogenesis of several human diseases. Thus identification of effective treatment is of great importance. Edible mushroom and microalgae are rich in the effective antioxidant phytochemicals. Hence, their beneficial effects on oxidative stress-associated inflammation are extremely required to be investigated. METHODS: This study evaluated the functional constituents, antioxidant and anti-inflammatory activities of Malaysian Ganoderma lucidum aqueous extract (GLE) and Egyptian Chlorella vulgaris ethanolic extract (CVE)...
May 10, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29744270/molecular-characterization-of-freshwater-microalgae-and-nutritional-exploration-to-enhance-their-lipid-yield
#10
Madan Kumar Mandal, Neha Chaurasia
Biomass and lipid yield under nutrient depleted and supplemented conditions of N and Mg in two freshwater isolates, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 from Meghalaya were investigated for biodiesel production. Both the strains, Chlorella sp. NC-MKM and Scenedesmus acutus NC-M2 are oleaginous in nature having lipid content of 40.2 ± 1.4 and 37.3 ± 2.6% DCW, respectively. The significant increase (92.8%) in lipid content was found in N-depleted condition while an increase (46.65%) in biomass yield was observed under Mg-supplemented condition in Chlorella sp...
May 2018: 3 Biotech
https://www.readbyqxmd.com/read/29735319/lipid-accumulation-of-chlorella-pyrenoidosa-under-mixotrophic-cultivation-using-acetate-and-ammonium
#11
Lijun Liu, Yuan Zhao, Xiaoxue Jiang, Xiaoyu Wang, Wenyan Liang
Acetate and ammonium were used as organic carbon and nitrogen sources, respectively, during mixotrophic cultivation of Chlorella pyrenoidosa. Cell growth, content of neutral lipid (NL), productivity of biomass and total lipid, and fatty acid profiles were investigated. Results showed that C. pyrenoidosa could endure high concentrations of NH4 + -N (100-200 mg/L) and immediately entered logarithmic growth, when the culture media contained 2.0-10.0 g/L NaAc. The 2.0-10.0 g/L NaAc in the media also resulted in the NL content of 1...
April 25, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29729644/medium-s-conductivity-and-stage-of-growth-as-crucial-parameters-for-efficient-hydrocarbon-extraction-by-electric-field-from-colonial-micro-algae
#12
Alexis Guionet, Bahareh Hosseini, Hidenori Akiyama, Hamid Hosano
The green algae Botryococcus braunii produces a high amount of extracellular hydrocarbon, making it a promising algae in the field of bio-fuels production. As it mainly produces squalene like hydrocarbons, cosmetic industries are also interested in its milking. Pulsed electric fields (PEF) are an innovative method allowing oil extraction from micro-algae. In common algae accumulating hydrocarbon inside cytoplasm (Chlorella vulgaris, Nannochloropsis sp., etc), electric fields can destroy cell membranes, allowing the release of hydrocarbon...
April 25, 2018: Bioelectrochemistry
https://www.readbyqxmd.com/read/29729541/comparative-uptake-study-of-arsenic-boron-copper-manganese-and-zinc-from-water-by-different-green-microalgae
#13
Ricardo Saavedra, Raúl Muñoz, María Elisa Taboada, Marisol Vega, Silvia Bolado
This work represents a comparative uptake study of the toxic elements arsenic, boron, copper, manganese and zinc in monometallic and multimetallic solutions by four green microalgae species (Chlamydomonas reinhardtii, Chlorella vulgaris, Scenedesmus almeriensis and an indigenous Chlorophyceae spp.), evaluating the effect of pH and contact time. Maximum removal efficiencies for each toxic element were 99.4% for Mn (C. vulgaris, pH 7.0, 3 h), 91.9% for Zn (Chlorophyceae spp., pH 5.5, 3 h), 88% for Cu (Chlorophyceae spp...
April 26, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29728971/arsenic-uptake-transformation-and-release-by-three-freshwater-algae-under-conditions-with-and-without-growth-stress
#14
Shaowen Xie, Jinxin Liu, Fen Yang, Hanxiao Feng, Chaoyang Wei, Fengchang Wu
This study was carried out using indoor controlled experiments to study the arsenic (As) uptake, biotransformation, and release behaviors of freshwater algae under growth stress. Three freshwater algae, Microcystis aeruginosa, Anabaena flosaquae, and Chlorella sp., were chosen. Two types of inhibitors, e.g., Cu2+ and isothiazolinone, were employed to inhibit the growth of the algae. The algae were cultivated to a logarithmic stage in growth media containing 0.1 mg/L P; then, 0.8 mg/L As in the form of arsenate (iAsV ) was added, while both inhibitors were simultaneously added at dosages of 0...
May 4, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29725885/ecotoxicological-assessment-of-the-herbicide-winner-top-and-its-active-substances-are-the-other-formulants-truly-inert
#15
Libânia Queirós, Tânia Vidal, António J A Nogueira, Fernando J M Gonçalves, Joana Luísa Pereira
Formulants used in Plant Protection Products (PPPs) to promote their efficiency are normally undisclosed in the PPP documentation, unless they bear a human health or environmental hazardous potential per se. PPP regulation also demands the assessment of putative interactions among formulants within each product recipe and consequent effects, but these results are often unavailable. Such a case is that of the herbicide Winner Top (Selectis®, Portugal), which we selected as a model commercial formulation in the present study specifically aiming at (i) characterising its aquatic toxicity towards sensitive eco-receptors (Raphidocelis subcapitata, Chlorella vulgaris, Lemna minor and Lemna gibba), as well as that of its active substances (a...
May 3, 2018: Ecotoxicology
https://www.readbyqxmd.com/read/29723963/bare-iron-oxide-nanoparticles-for-magnetic-harvesting-of-microalgae-from-interaction-behavior-to-process-realization
#16
Paula Fraga-García, Peter Kubbutat, Markus Brammen, Sebastian Schwaminger, Sonja Berensmeier
Microalgae continue to gain in importance as a bioresource, while their harvesting remains a major challenge at the moment. This study presents findings on microalgae separation using low-cost, easy-to-process bare iron oxide nanoparticles with the additional contribution of the upscaling demonstration of this simple, adhesion-based process. The high affinity of the cell wall for the inorganic surface enables harvesting efficiencies greater than 95% for Scenedesmus ovalternus and Chlorella vulgaris . Successful separation is possible in a broad range of environmental conditions and primarily depends on the nanoparticle-to-microalgae mass ratio, whereas the effect of pH and ionic strength are less significant when the mass ratio is chosen properly...
May 1, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29723844/effect-of-combining-adsorption-stripping-treatment-with-acidification-on-the-growth-of-chlorella-vulgaris-and-nutrient-removal-from-swine-wastewater
#17
Leipeng Cao, Ting Zhou, Zihan Li, Jingjing Wang, Juan Tang, Roger Ruan, Yuhuan Liu
After swine wastewater (SW) was treated with adsorption-stripping stage, the concentration of NH4 + -N and Total phosphorus (TP) in SW significantly decreased from 598.04, 42.95 to 338.02, 8.36 mg L-1 , respectively. The concentration of heavy metals, especially Zn2+ (96.78%), decreased by the ion exchange of artificial zeolite (AZ). The acidification of SW could significantly improve the nutrient utilization efficiency and promote the growth rate of C. vulgaris due to the hydrolysis of macromolecular substances into smaller molecules usable for algae...
April 25, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29716405/-microalgae-as-the-source-of-natural-products
#18
Gábor Vasas
More than 90% of herbal products and herbal medicines have been derived from higher plants recently, but due to independent circumstances, several photosynthetic microalgal species are in focus in this point of view. In the last 50 years, many carbohydrate-, peptide-, terpenoid-, alkaloid- and phenol-type components were described from algae because of the developing structural determination and analytical methods, algae mass production and also artificial algae technologies. At the same time, based partly on traditional causes and partly on the clinical and preclinical data of today, some dried products of algae are directly used as food supplements...
May 2018: Orvosi Hetilap
https://www.readbyqxmd.com/read/29715637/effects-of-nutrient-ratios-and-carbon-dioxide-bio-sequestration-on-biomass-growth-of-chlorella-sp-in-bubble-column-photobioreactor
#19
Hoang-Nhat-Phong Vo, Xuan-Thanh Bui, Thanh-Tin Nguyen, Dinh Duc Nguyen, Thanh-Son Dao, Ngoc-Dan-Thanh Cao, Thi-Kim-Quyen Vo
Photobioreactor technology, especially bubble column configuration, employing microalgae cultivation (e.g., Chlorella sp.), is an ideal man-made environment to achieve sufficient microalgae biomass through its strictly operational control. Nutrients, typically N and P, are necessary elements in the cultivation process, which determine biomass yield and productivity. Specifically, N:P ratios have certain effects on microalgae's biomass growth. It is also attractive that microalgae can sequester CO2 by using that carbon source for photosynthesis and, subsequently, reducing CO2 emission...
April 28, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29700746/exploring-an-in-situ-led-illuminated-isothermal-micro-calorimetric-method-to-investigating-the-thermodynamic-behavior-of-chlorella-vulgaris-during-co-2-bio-fixation
#20
Mohammad Russel, Changrui Liu, Asraful Alam, Fei Wang, Jun Yao, Maurycy Daroch, Mahfuzur Rahman Shah, Zhongming Wang
Much endeavor has been dispensed recently to evaluate the potential of CO2 mitigation by microalgae. We introduce an alternative, novel, LED-illumination isothermal microcalorimetric method to assess the thermodynamic behaviors of microalgae for better understanding of their carbon sequestration capacity. Microalgae thermodynamic behaviors were recorded as power-time curves, and their indices such as total heat evolution (Q T ), maximum power output (Pmax ) and heat generated by per algae cell (J N/Q ) were obtained...
April 26, 2018: Environmental Science and Pollution Research International
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