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https://www.readbyqxmd.com/read/28093676/aryl-hydrocarbon-receptor-signaling-involved-in-the-invasiveness-of-lncap-cells
#1
Hisamitsu Ide, Yan Lu, Jingsong Yu, Takahiro Noguchi, Mayuko Kanayama, Satoru Muto, Raizo Yamaguchi, Suguru Kawato, Shigeo Horie
There is now mounting evidence that the aryl hydrocarbon receptor (AhR) plays an important role in physiologic responses such as development, cell cycle regulation, immune function and also malignant transformation in various tissues. The strong nuclear AhR expression is observed in the invasive phenotype, and an elevated nuclear AhR expression is associated with a poor prognosis of human prostate cancer. On the other hand, there are conflicting results that the AhR deficiency results in increased susceptibility to prostate tumors in mouse model...
January 16, 2017: Human Cell
https://www.readbyqxmd.com/read/28091582/androgen-mediated-regulation-of-endoplasmic-reticulum-associated-degradation-and-its-effects-on-prostate-cancer
#2
Yalcin Erzurumlu, Petek Ballar
The endoplasmic reticulum (ER) comprises thirty percent of the newly translated proteins in eukaryotic cells. The quality control mechanism within the ER distinguishes between properly and improperly folded proteins and ensures that unwanted proteins are retained in the ER and subsequently degraded through ER-associated degradation (ERAD). Besides cleaning of misfolded proteins ERAD is also important for physiological processes by regulating the abundance of normal proteins of the ER. Thus it is important to unreveal the regulation patterns of ERAD...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28078560/knockdown-of-pycr1-inhibits-cell-proliferation-and-colony-formation-via-cell-cycle-arrest-and-apoptosis-in-prostate-cancer
#3
Tengyue Zeng, Libing Zhu, Min Liao, Wenli Zhuo, Shunliang Yang, Weizhen Wu, Dong Wang
Pyrroline-5-carboxylate reductase 1 (PYCR1) is an enzyme involved in cell metabolism, which has been shown to be up-regulated in cancers. However, the functions of PYCR1 in prostate cancers (PCa) are still largely unknown. In the present study, we found that PYCR1 was highly expressed in prostate cancer tissues and then knocked down PYCR1 in PCa cell lines (DU145, PC-3 and LNCap) via lentivirus-mediated gene delivery and analyzed its biological function. Both qRT-PCR and western blotting indicated that PYCR1 was suppressed efficiently after sh-PYCR1 infection...
February 2017: Medical Oncology
https://www.readbyqxmd.com/read/28076853/pyruvate-dehydrogenase-expression-is-negatively-associated-with-cell-stemness-and-worse-clinical-outcome-in-prostate-cancers
#4
Yali Zhong, Xiaoli Li, Yasai Ji, Xiaoran Li, Yaqing Li, Dandan Yu, Yuan Yuan, Jian Liu, Huixiang Li, Mingzhi Zhang, Zhenyu Ji, Dandan Fan, Jianguo Wen, Mariusz Adam Goscinski, Long Yuan, Bin Hao, Jahn M Nesland, Zhenhe Suo
Cells generate adenosine-5'-triphosphate (ATP), the major currency for energy-consuming reactions, through mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis. One of the remarkable features of cancer cells is aerobic glycolysis, also known as the "Warburg Effect", in which cancer cells rely preferentially on glycolysis instead of mitochondrial OXPHOS as the main energy source even in the presence of high oxygen tension. One of the main players in controlling OXPHOS is the mitochondrial gatekeeperpyruvate dehydrogenase complex (PDHc) and its major subunit is E1α (PDHA1)...
January 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28063792/syntheses-and-antiproliferative-activities-of-novel-phosphatidylcholines-containing-dehydroepiandrosterone-moieties
#5
Marek Kłobucki, Aleksandra Grudniewska, Damian A Smuga, Małgorzata Smuga, Joanna Jarosz, Joanna Wietrzyk, Gabriela Maciejewska, Czesław Wawrzeńczyk
Dehydroepiandrosterone (DHEA) is a natural hormone with many beneficial properties including an anticancer activity. Unfortunately, DHEA is unstable in the body and exhibits cytotoxicity against healthy cells. In this study, a series of new phosphocholines containing DHEA at sn-1 and/or sn-2 positions were prepared. Succinic acid was used as a linker between the active drug and sn-glycero-3-phosphocholine. All the compounds were evaluated in vitro for their antiproliferative activities against four cell lines: Balb/3T3, HL-60, B16, and LNCaP...
January 4, 2017: Steroids
https://www.readbyqxmd.com/read/28057407/bifunctional-conjugates-with-potent-inhibitory-activity-towards-cyclooxygenase-and-histone-deacetylase
#6
Idris Raji, Fatima Yadudu, Emily Janeira, Shaghayegh Fathi, Lindsey Szymczak, James Richard Kornacki, Kensei Komatsu, Jian-Dong Li, Milan Mrksich, Adegboyega K Oyelere
We herein disclose a series of compounds with potent inhibitory activities towards histone deacetylases (HDAC) and cyclooxygenases (COX). These compounds potently inhibited the growth of cancer cell lines consistent with their anti-COX and anti-HDAC activities. While compound 2b showed comparable level of COX-2 selectivity as celecoxib, compound 11b outperformed indomethacin in terms of selectivity towards COX-2 relative to COX-1. An important observation with our lead compounds (2b, 8, 11b, and 17b) is their enhanced cytotoxicity towards androgen dependent prostate cancer cell line (LNCaP) relative to androgen independent prostate cancer cell line (DU-145)...
December 24, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28055971/src-promotes-castration-recurrent-prostate-cancer-through-androgen-receptor-dependent-canonical-and-non-canonical-transcriptional-signatures
#7
Indranil Chattopadhyay, Jianmin Wang, Maochun Qin, Lingqiu Gao, Renae Holtz, Robert L Vessella, Robert W Leach, Irwin H Gelman
Progression of prostate cancer (PC) to castration-recurrent growth (CRPC) remains dependent on sustained expression and transcriptional activity of the androgen receptor (AR). A major mechanism contributing to CRPC progression is through the direct phosphorylation and activation of AR by Src-family (SFK) and ACK1 tyrosine kinases. However, the AR-dependent transcriptional networks activated by Src during CRPC progression have not been elucidated. Here, we show that activated Src (Src527F) induces androgen-independent growth in human LNCaP cells, concomitant with its ability to induce proliferation/survival genes normally induced by dihydrotestosterone (DHT) in androgen-dependent LNCaP and VCaP cells...
December 31, 2016: Oncotarget
https://www.readbyqxmd.com/read/28054089/separation-and-dual-detection-of-prostate-cancer-cells-and-protein-biomarkers-using-a-microchip-device
#8
Wanfeng Huang, Chun-Li Chang, Norman D Brault, Onur Gur, Zhe Wang, Shadia I Jalal, Philip S Low, Timothy L Ratliff, Roberto Pili, Cagri A Savran
Current efforts for the detection of prostate cancer using only prostate specific antigen are not ideal and indicate a need to develop new assays - using multiple targets - that can more accurately stratify disease states. We previously introduced a device capable of the concurrent detection of cellular and molecular markers from a single sample fluid. Here, an improved design, which achieves affinity as well as size-based separation of captured targets using antibody-conjugated magnetic beads and a silicon chip containing micro-apertures, is presented...
January 5, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28052036/angiomotin-regulates-prostate-cancer-cell-proliferation-by-signaling-through-the-hippo-yap-pathway
#9
Hao Zeng, Angelica Ortiz, Peng-Fei Shen, Chien-Jui Cheng, Yu-Chen Lee, Guoyu Yu, Song-Chang Lin, Chad J Creighton, Li-Yuan Yu-Lee, Sue-Hwa Lin
Angiomotin (AMOT) is a family of proteins found to be a component of the apical junctional complex of vertebrate epithelial cells and is recently found to play important roles in neurofibromatosis type 2 (NF-2). Whether AMOT plays a role in prostate cancer (PCa) is unknown. AMOT is expressed as two isoforms, AMOTp80 and AMOTp130, which has a 409 aa N-terminal domain that is absent in AMOTp80. Both AMOTp80 and AMOTp130 are expressed in LNCaP and C4-2B4, but at a low to undetectable level in PC3, DU145, and BPH1 cells...
December 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/28051996/full-preclinical-validation-of-the-123i-labeled-anti-psma-antibody-fragment-scfvd2b-for-prostate-cancer-imaging
#10
Barbara Frigerio, Gerben Franssen, Elena Luison, Alessandro Satta, Ettore Seregni, Marco Colombatti, Giulio Fracasso, Riccardo Valdagni, Delia Mezzanzanica, Otto Boerman, Silvana Canevari, Mariangela Figini
PURPOSE: In the context of prostate cancer (PCa) imaging, the aim of this study was to optimize (in vitro) the specificity and assess preclinically (in vivo) the tumor targeting properties of the 123I-scFvD2B antibody specific for prostate-specific membrane antigen (PSMA). EXPERIMENTAL DESIGN: The 123I-labeling conditions of the antibody fragment scFvD2B, produced in an eukaryotic system under GMP-compliant conditions, were optimized and assessed for purity and immunoreactivity...
December 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/28048451/we-ab-bra-02-study-of-the-therapeutic-effects-of-pulsed-focused-ultrasound-combined-with-encapsulated-chemotherapeutic-agents-for-prostate-cancer-in-vivo
#11
L Chen, D Cvetkovic, X Chen, B Wang, R Gupta, C Ma
PURPOSE: To investigate the improvement of prostate cancer inhibition by combining pulsed focused ultrasound (pFUS) exposures and chemotherapeutic agents encapsulated nanodroplets under MR guidance for prostate cancer therapy. METHODS: Prostate cancer (LNCaP) cells were implanted orthotopically. First we developed nanodroplets encapsulated with chemotherapeutic agents for both paclitaxel (PTX) and docetaxel (DTX). Secondly, tumor-bearing mice were randomly divided into 5 groups (n=5)...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048178/su-f-t-674-in-vitro-study-of-5-aminolevulinic-acid-mediated-photo-dynamic-therapy-in-human-cancer-cell-lines
#12
D Cvetkovic, B Wang, R Gupta, C Ma
PURPOSE: Photodynamic therapy (PTD) is a promising cancer treatment modality. 5-sminolevulinic acid (ALA) is a clinically approved photosensitizer. Here we studied the effect of 5-ALA administration with irradiation on several cell lines in vitro. METHODS: Human head and neck (FaDu), lung (A549) and prostate (LNCaP) cancer cells (104/well) were seeded overnight in 96-well plates (Figure 1). 5-ALA at a range from 0.1 to 30.0mg/ml was added to confluent cells 3h before irradiation in 100ul of culture medium...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28048114/su-f-j-216-mr-guided-non-thermal-pulsed-focused-ultrasound-for-cancer-therapy
#13
C Ma, B Wang, D Cvetkovic, L Chen
PURPOSE: High-intensity focused ultrasound has been investigated for ablative therapy and drug enhancement for gene therapy and chemotherapy. The aim of this work is to explore the feasibility of pulsed focused ultrasound (pFUS) for non-thermal cancer therapy using an in vivo animal model. METHODS: An InSightec ExAblate 2000 with a 1.5T GE MR scanner was used in this study. Suitable ultrasound parameters were investigated to perform non-thermal sonications. LNCaP human prostate tumor cells (106) were injected orthotopically in the prostates of nude mice (n = 16)...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046739/su-f-j-225-histology-study-of-mr-guided-pulsed-focused-ultrasound-on-treatment-of-prostate-cancer-in-vivo
#14
L Chen, D Cvetkovic, X Chen, B Wang, R Gupta, C Ma
PURPOSE: Our previous study demonstrated significant tumor growth delay in the mice treated with pulsed high intensity focused ultrasound (pHIFU). The purpose of this study is to understand the cell killing mechanisms of pHIFU. METHODS: Prostate cancer cells (LNCaP), were grown orthotopically in 17 nude mice. Tumor-bearing mice were treated using pHIFU with an acoustic power of 25W, pulse width 100msec and 300 pulses in one sonication under MR guidance. Mutiple sonications were used to cover the whole tumor volume...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046379/we-ab-bra-01-biodistribution-of-paclitaxel-loaded-nanodroplets-for-prostate-cancer-management-using-ultrasound-mediated-drug-delivery-under-mrguidance
#15
R Gupta, D Cvetkovic, B Wang, L Chen, C Ma
PURPOSE: To study the biodistribution of paclitaxel-loaded nanodroplets in vivo in order to evaluate the efficacy of focused ultrasound (FUS)-mediated drug delivery under MR-guidance to prostate tumor. METHODS: Poly {ethylene oxide}-co-poly {D, L-lactide} (PDLA) nanodroplets loaded with fluorescently labeled-paclitaxel (F-PTX) were synthesized using solid dispersion technique. Human prostate cancer, LNCaP cells were implanted orthotopically in prostates of male nude mice...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28038451/androgen-receptor-transcriptionally-regulates-semaphorin-3c-in-a-gata2-dependent-manner
#16
Kevin J Tam, Kush Dalal, Michael Hsing, Chi Wing Cheng, Shahram Khosravi, Parvin Yenki, Charan Tse, James W Peacock, Aishwariya Sharma, Yan Ting Chiang, Yuzhuo Wang, Artem Cherkasov, Paul S Rennie, Martin E Gleave, Christopher J Ong
The androgen receptor (AR) is a member of the nuclear receptor superfamily of transcription factors and is central to prostate cancer (PCa) progression. Ligand-activated AR engages androgen response elements (AREs) at androgen-responsive genes to drive the expression of gene batteries involved in cell proliferation and cell fate. Understanding the transcriptional targets of the AR has become critical in apprehending the mechanisms driving treatment-resistant stages of PCa. Although AR transcription regulation has been extensively studied, the signaling networks downstream of AR are incompletely described...
December 25, 2016: Oncotarget
https://www.readbyqxmd.com/read/28035996/novel-nine-exon-ar-transcripts-exon-1-exon-1b-exons-2-8-in-normal-and-cancerous-breast-and-prostate-cells
#17
Dong Gui Hu, Ross A McKinnon, Julie-Ann Hulin, Peter I Mackenzie, Robyn Meech
Nearly 20 different transcripts of the human androgen receptor (AR) are reported with two currently listed as Refseq isoforms in the NCBI database. Isoform 1 encodes wild-type AR (type 1 AR) and isoform 2 encodes the variant AR45 (type 2 AR). Both variants contain eight exons: they share common exons 2-8 but differ in exon 1 with the canonical exon 1 in isoform 1 and the variant exon 1b in isoform 2. Splicing of exon 1 or exon 1b is reported to be mutually exclusive. In this study, we identified a novel exon 1b (1b/TAG) that contains an additional TAG trinucleotide upstream of exon 1b...
December 27, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28035382/short-hairpin-rna-directed-against-%C3%AE-catenin-inhibits-prostate-cancer-growth-and-invasion-in%C3%A2-vitro
#18
Jiahui Zhao, Chunting Wu, Yong Luo, Yongguang Jiang
β-catenin protein exhibits a dual function in epithelial cells, depending on its intracellular localization. At the plasma membrane, β‑catenin is an important constituent of adherens junctions. However, when the Wnt/β‑catenin signaling pathway is activated, β‑catenin translocates to the nucleus to promote specific gene expression. To investigate the functional activity and examine the role of the Wnt/β‑catenin signaling pathway in various human prostate cancer cells, indirect immunofluorescence was performed to detect the expression and distribution of β‑catenin in the following prostate cancer cell lines: PC‑3, LNCaP, C4‑2, IA8‑ARCaP and IF11‑ARCaP...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28032932/androgen-induced-lncrna-potef-as1-regulates-apoptosis-related-pathway-to-facilitate-cell-survival-in-prostate-cancer-cells
#19
Aya Misawa, Ken-Ichi Takayama, Tetsuya Fujimura, Yukio Homma, Yutaka Suzuki, Satoshi Inoue
Although long noncoding RNAs (lncRNA) have been associated with a variety of cancers, the interplay between lncRNAs and androgen receptor (AR) signaling in prostate cancer is still unclear. Here, we identified an androgen-dependent lncRNA, prostate, ovary, testis expressed protein family member-F antisense transcript-1 (POTEF-AS1), whose expression was regulated by AR in two androgen-dependent cells by using directional RNA-seq analysis. POTEF-AS1 promoted cell growth, repressed genes related to the toll-like receptor (TLR) signaling and apoptosis pathways and inhibited apoptosis in docetaxel-treated LNCaP cells...
December 29, 2016: Cancer Science
https://www.readbyqxmd.com/read/28032594/structurally-related-odorant-ligands-of-the-olfactory-receptor-or51e2-differentially-promote-metastasis-emergence-and-tumor-growth
#20
Guenhaël Sanz, Isabelle Leray, Denise Grébert, Sharmilee Antoine, Adrien Acquistapace, Adeline Muscat, Abdelhak Boukadiri, Lluis M Mir
Olfactory receptors are G protein-coupled receptors. Some of them are expressed in tumor cells, such as the OR51E2 receptor overexpressed in LNCaP prostate cancer cells. It is considered a prostate tumor marker. We previously demonstrated that this receptor is able to promote LNCaP cell invasiveness in vitro upon stimulation with its odorant agonist β-ionone, leading to increased generation of metastases in vivo. In the present study, we show that even a relatively short exposure to β-ionone is sufficient to promote metastasis emergence...
December 9, 2016: Oncotarget
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