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https://www.readbyqxmd.com/read/28918339/interference-of-dibutylphthalate-on-human-prostate-cell-viability
#1
Mariana Di Lorenzo, Maurizio Forte, Salvatore Valiante, Vincenza Laforgia, Maria De Falco
Dibutylphthalate (DBP) is an environmental pollutant widely used as plasticizer in a variety of industrial applications worldwide. This agent can be found in personal-care products, children's toy, pharmaceuticals, food products. Exposure to DBP can occur via ingestion and inhalation as well as intravenous or skin contact. DBP belongs to the family of endocrine disrupting chemicals (EDCs) and its effects on reproductive system were demonstrated both in vivo and in vitro. In the present study we evaluated the effects of DBP on human prostate adenocarcinoma epithelial cells (LNCaP) in order to highlight xenoestrogens influence on human prostate...
September 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28916296/folate-targeted-amphiphilic-cyclodextrin-nanoparticles-incorporating-a-fusogenic-peptide-deliver-therapeutic-sirna-and-inhibit-the-invasive-capacity-of-3d-prostate-cancer-tumours
#2
James C Evans, Meenakshi Malhotra, Katrina Sweeney, Raphael Darcy, Colleen C Nelson, Brett G Hollier, Caitriona M O'Driscoll
The main barrier to the development of an effective RNA interference (RNAi) therapy is the lack of a suitable delivery vector. Modified cyclodextrins have emerged in recent years for the delivery of siRNA. In the present study, a folate-targeted amphiphilic cyclodextrin was formulated using DSPE-PEG5000-folate to target prostate cancer cells. The fusogenic peptide GALA was included in the formulation to aid in the endosomal release of siRNA. Targeted nanoparticles were less than 200nm in size with a neutral surface charge...
September 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28915670/prostate-cancer-diagnosis-using-epigenetic-biomarkers-3d-high-content-imaging-and-probabilistic-cell-by-cell-classifiers
#3
Darko Stefanovski, George Tang, Kolja Wawrowsky, Raymond C Boston, Nils Lambrecht, Jian Tajbakhsh
BACKGROUND: Prostate cancer (PCa) management can benefit from novel concepts/biomarkers for reducing the current 20-30% chance of false-negative diagnosis with standard histopathology of biopsied tissue. METHOD: We explored the potential of selected epigenetic markers in combination with validated histopathological markers, 3D high-content imaging, cell-by-cell analysis, and probabilistic classification in generating novel detailed maps of biomarker heterogeneity in patient tissues, and PCa diagnosis...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28915623/inhibition-of-the-pi3k-akt-mtor-pathway-activates-autophagy-and-compensatory-ras-raf-mek-erk-signalling-in-prostate-cancer
#4
Dominika E Butler, Christopher Marlein, Hannah F Walker, Fiona M Frame, Vincent M Mann, Matthew S Simms, Barry R Davies, Anne T Collins, Norman J Maitland
The PI3K/AKT/mTOR pathway is frequently activated in advanced prostate cancer, due to loss of the tumour suppressor PTEN, and is an important axis for drug development. We have assessed the molecular and functional consequences of pathway blockade by inhibiting AKT and mTOR kinases either in combination or as individual drug treatments. In established prostate cancer cell lines, a decrease in cell viability and in phospho-biomarker expression was observed. Although apoptosis was not induced, a G1 growth arrest was observed in PTEN null LNCaP cells, but not in BPH1 or PC3 cells...
August 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28913790/delivery-of-hsp90-inhibitor-using-water-soluble-polymeric-conjugates-with-high-drug-payload
#5
Jose A Suárez Del Pino, Rohit Kolhatkar
PURPOSE: HSP90 (Heat shock protein 90kD) has been validated as a therapeutic target in Castrate Resistant Prostate Cancer. Unfortunately, HSP90 inhibitors suffer from dose-limiting toxicities that hinder their clinical applications. Previously developed polymeric delivery systems for HSP90 inhibitors had either low drug content or low biological activity suggesting the need for better delivery system for HSP90 inhibitors. METHODS: We developed a simplified synthetic strategy to prepare polyethylene glycol based water-soluble polymeric system for model HSP90 inhibitor geldanamycin (GDM)...
September 14, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28905415/hyperactive-mtor-induces-neuroendocrine-differentiation-in-prostate-cancer-cell-with-concurrent-up-regulation-of-irf1
#6
Mayuko Kanayama, Toshiya Hayano, Michinori Koebis, Tatsuya Maeda, Yoko Tabe, Shigeo Horie, Atsu Aiba
BACKGROUND: Neuroendocrine-differentiated prostate cancer (NEPCa) is refractory to androgen deprivation therapy and shows a poor prognosis. The underlying mechanisms responsible for neuroendocrine differentiation (NED) are yet to be clarified. In this study, we investigated the role of mammalian target of rapamycin (mTOR) in NEPCa. METHODS: We utilized a gain-of-function analysis by establishing a human PCa LNCaP stable line that expresses hyperactive mTOR (LNCaP-mTOR)...
September 14, 2017: Prostate
https://www.readbyqxmd.com/read/28901514/multimodal-actions-of-the-phytochemical-sulforaphane-suppress-both-ar-and-ar-v7-in-22rv1-cells-advocating-a-potent-pharmaceutical-combination-against-castration-resistant-prostate-cancer
#7
Namrata Khurana, Hogyoung Kim, Partha K Chandra, Sudha Talwar, Pankaj Sharma, Asim B Abdel-Mageed, Suresh C Sikka, Debasis Mondal
Prostate cancer (PCa) cells expressing full-length androgen receptor (AR-FL) are susceptible to androgen deprivation therapy (ADT). However, outgrowth of castration-resistant prostate cancer (CRPC) can occur due to the expression of constitutively active (ligand-independent) AR splice variants, particularly AR-V7. We previously demonstrated that sulforaphane (SFN), an isothiocyanate phytochemical, can decrease AR-FL levels in the PCa cell lines, LNCaP and C4-2B. Here, we examined the efficacy of SFN in targeting both AR-FL and AR-V7 in the CRPC cell line, CWR22Rv1 (22Rv1)...
August 30, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28901378/androgen-independent-lncap-cells-are-a-subline-of-lncap-cells-with-a-more-aggressive-phenotype-and-androgen-suppresses-their-growth-by-inducing-cell-cycle-arrest-at-the-g1%C3%A2-phase
#8
Pan Yu, Xiuzhi Duan, Yue Cheng, Chunhua Liu, Yuhua Chen, Weiwei Liu, Binbin Yin, Xuchu Wang, Zhihua Tao
Androgen deprivation therapy (ADT, surgical or chemical castration) is the mainstay treatment for metastatic prostate cancer (PCa); however, patients ineluctably relapse despite extremely low androgen levels. This evolution of PCa indicates its lethal progression. In this study, to mimic the traits of clinical PCa progression in vitro, we investigated the alterations in the cell biological characteristics in androgen-independent LNCaP cells (LNCaP-AI cells) compared with LNCaP cells. We also examined the effects of androgen on LNCaP and LNCaP-AI cell proliferation, androgen receptor (AR) expression and prostate-specific antigen (PSA) secretion...
September 7, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28900896/fusion-of-gelonin-and-anti-insulin-like-growth-factor-1-receptor-igf-1r-affibody-for-enhanced-brain-cancer-therapy
#9
Songhee Ham, Kyoung Ah Min, Jae Wook Yang, Meong Cheol Shin
Owing to the extraordinary potency in inhibiting protein translation that could eventually lead to apoptosis of tumor cells, ribosome-inactivating proteins (RIPs) such as gelonin have been considered attractive drug candidates for cancer therapy. However, due to several critical obstacles (e.g., severe toxicity issues caused by a lack of selectivity in their mode of action and the low cytotoxicity via poor cellular uptake, etc.), clinical application of RIPs is yet far from being accomplished. To overcome these challenges, in the present study, we engineered gelonin fusion proteins with anti-insulin-like growth factor-1 receptor (IGF-1R) affibody ("IAFF") via the genetic recombinant method and the SpyCatcher/SpyTag-mediated conjugation method...
September 12, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28890397/regulated-expression-of-the-tp%C3%AE-isoform-of-the-human-t-prostanoid-receptor-by-the-tumour-suppressors-foxp1-and-nkx3-1-implications-for-the-role-of-thromboxane-in-prostate-cancer
#10
Aine G O'Sullivan, Sarah B Eivers, Eamon P Mulvaney, B Therese Kinsella
The prostanoid thromboxane (TX)A2 signals through the TPα and TPβ isoforms of T Prostanoid receptor (TP) that are transcriptionally regulated by distinct promoters termed Prm1 and Prm3, respectively, within the TBXA2R gene. We recently demonstrated that expression of TPα and TPβ is increased in PCa, differentially correlating with Gleason grade and with altered CpG methylation of the individual Prm1/Prm3 regions within the TBXA2R. The current study sought to localise the sites of CpG methylation within Prm1 and Prm3, and to identify the main transcription factors regulating TPβ expression through Prm3 in the prostate adenocarcinoma PC-3 and LNCaP cell lines...
September 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28889753/silencing-of-bach1-inhibits-invasion-and-migration-of-prostate-cancer-cells-by-altering-metastasis-related-gene-expression
#11
Neda Shajari, Sadaf Davudian, Tohid Kazemi, Behzad Mansoori, Shima Salehi, Vahid Khaze Shahgoli, Dariush Shanehbandi, Ali Mohammadi, Pascal H G Duijf, Behzad Baradaran
BACKGROUND: Cancer lethality is mainly caused by metastasis. Therefore, understanding the nature of the genes involved in this process has become a priority. BACH1, a basic leucine zipper transcription factor, has been shown to transcriptionally regulate expression of a range of genes that are associated with breast cancer metastasis. However, the exact role and the underlying molecular mechanism of BACH1 in prostate cancer remain unclear. This study aims to explore the expression of BACH1 in prostate cancer tissues and the effect of BACH1 suppression on prostate cancer cell behavior...
September 11, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28888997/discovery-of-peptide-drug-carrier-candidates-for-targeted-multi-drug-delivery-into-prostate-cancer-cells
#12
O Bashari, B Redko, A Cohen, G Luboshits, G Gellerman, M A Firer
Metastatic castration-resistant prostate cancer (mCRPC) remains essentially incurable. Targeted Drug Delivery (TDD) systems may overcome the limitations of current mCRPC therapies. We describe the use of strict criteria to isolate novel prostate cancer cell targeting peptides that specifically deliver drugs into target cells. Phage from a libraries displaying 7mer peptides were exposed to PC-3 cells and only internalized phage were recovered. The ability of these phage to internalize into other prostate cancer cells (LNCaP, DU-145) was validated...
September 6, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28888989/noradrenaline-oxymetazoline-and-phorbol-myristate-acetate-induce-distinct-functional-actions-and-phosphorylation-patterns-of-%C3%AE-1a-adrenergic-receptors
#13
Rocío Alcántara-Hernández, Aurelio Hernández-Méndez, M Teresa Romero-Ávila, Marco A Alfonzo-Méndez, André S Pupo, J Adolfo García-Sáinz
In LNCaP cells that stably express α1A-adrenergic receptors, oxymetazoline increased intracellular calcium and receptor phosphorylation, however, this agonist was a weak partial agonist, as compared to noradrenaline, for calcium signaling. Interestingly, oxymetazoline-induced receptor internalization and desensitization displayed greater effects than those induced by noradrenaline. Phorbol myristate acetate induced modest receptor internalization and minimal desensitization. α1A-Adrenergic receptor interaction with β-arrestins (colocalization/coimmunoprecipitation) was induced by noradrenaline and oxymetazoline and, to a lesser extent, by phorbol myristate acetate...
September 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28888979/resveratrol-piceatannol-and-analogs-inhibit-activation-of-both-wild-type-and-t877a-mutant-androgen-receptor
#14
Johan Lundqvist, Corrado Tringali, Agneta Oskarsson
Prostate cancer growth and progression is mainly dependent on androgens and many current prostate cancer treatment options target the synthesis or function of androgens. We have previously reported that resveratrol and synthetic analogs of resveratrol with a higher bioavailability inhibit the synthesis of androgens in human adrenocortical H295R cells. Now we have studied the antiandrogenic properties of resveratrol, piceatannol and analogs in two different prostate cell lines; LNCaP and RWPE. LNCaP carry a T877A mutation in the androgen receptor while RWPE has a wild-type androgen receptor...
September 6, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28886322/carnosol-inhibits-hedgehog-signaling-pathway-in-both-lncap-and-du145-prostate-cancer-cell-lines
#15
H Gao, Q Song, J Yang, S Yu, J Zhao, G Yu
To investigate the effect of carnosol on the Hedgehog (HH) signaling pathway in human hormone-dependent prostate cancer cell line LNCaP and hormone-independent prostate cancer cell line DU145. The expression levels of glioma-associated oncogene homolog 1 (Gli1) and Sonic hedgehog (Shh) in human prostate cancer tissues were detected by immunohistochemistry. After treated with carnosol (0.25-16 μmol/L), the cell survival of LNCaP and DU145 cells were detected by MTT assay. The expression levels of Gli1 and Shh mRNA and protein in the two cells were detected by qRT-PCR and western blot, respectively...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28881808/cxcl13-is-androgen-responsive-and-involved-in-androgen-induced-prostate-cancer-cell-migration-and-invasion
#16
Long Fan, Qingyi Zhu, Li Liu, Cuicui Zhu, Haojie Huang, Shan Lu, Ping Liu
Androgen receptor (AR) is a key transcription factor playing a critical role in prostate cancer (PCa) initiation and progression. However, the molecular mechanisms of AR action in prostate cancer are not very clear. CXCL13, known as B cell attracting chemokine1 (BCA-1), is a member of CXC chemokine family and relevant to cancer metastasis. This study shows that CXCL13 is an androgen-responsive gene and involved in AR-induced PCa cell migration and invasion. In clinical specimens, expression of CXCL13 in PCa tissues is markedly higher than that in adjacent normal tissues...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28881726/modulation-of-paracrine-signaling-by-cd9-positive-small-extracellular-vesicles-mediates-cellular-growth-of-androgen-deprived-prostate-cancer
#17
Carolina Soekmadji, James D Riches, Pamela J Russell, Jayde E Ruelcke, Stephen McPherson, Chenwei Wang, Chris M Hovens, Niall M Corcoran, Michelle M Hill, Colleen C Nelson
Proliferation and maintenance of both normal and prostate cancer (PCa) cells is highly regulated by steroid hormones, particularly androgens, and the extracellular environment. Herein, we identify the secretion of CD9 positive extracellular vesicles (EV) by LNCaP and DUCaP PCa cells in response to dihydrotestosterone (DHT) and use nano-LC-MS/MS to identify the proteins present in these EV. Subsequent bioinformatic and pathway analyses of the mass spectrometry data identified pathologically relevant pathways that may be altered by EV contents...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28880012/golph2-regulated-oncolytic-adenovirus-gd55-exerts-strong-killing-effect-on-human-prostate-cancer-stem-like-cells-in-vitro-and-in-vivo
#18
Chang Ying, Bo-Duan Xiao, Yun Qin, Bin-Rong Wang, Xin-Yuan Liu, Ru-Wei Wang, Ling Fang, Hui Yan, Xiu-Mei Zhou, Yi-Gang Wang
GOLPH2 (also called GP73) is a Golgi glycoprotein, which has been identified as a novel tumor marker upregulated in various cancers, including prostate cancer (PCa). GD55 is a novel GOLPH2-regulated oncolytic adenovirus that exhibits a strong killing effect on hepatoma cells. Here, we investigate the antitumor effect of GD55 on prostate cancer stem cell (CSC)-like cells in vitro and in vivo. Prostate CSC-like sphere cells were acquired and enriched by culturing DU145, LNCap or P3 prostate cancer cells in suspension...
September 7, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28876465/procyanidin-b2-3-3-di-o-gallate-induces-oxidative-stress-mediated-cell-death-in-prostate-cancer-cells-via-inhibiting-map-kinase-phosphatase-activity-and-activating-erk1-2-and-ampk
#19
Rahul Kumar, Gagan Deep, Michael F Wempe, Joseph Surek, Amit Kumar, Rajesh Agarwal, Chapla Agarwal
Neoplastic cells exhibit higher oxidative stress compared to normal cells; however, antioxidants based clinical trials have mostly failed. Another attractive therapeutic approach is to further increase the oxidative stress in cancer cells leading to cell death. Herein, we show that Procyanidin B2 3,3"-di-O-gallate (B2G2), the most active constituent of grape seed extract, treatment causes cell death in human prostate cancer (PCa) cells (LNCaP and 22Rv1) via increasing the reactive oxygen species (ROS) generation...
September 6, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28869219/andrographolide-sensitizes-prostate-cancer-cells-to-trail-induced-apoptosis
#20
Ruo-Jing Wei, Xin-Shi Zhang, Da-Lin He
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising agent for anticancer therapy. The identification of small molecules that can establish the sensitivity of prostate cancer (PCa) cells to TRAIL-induced apoptosis is crucial for the targeted treatment of PCa. PC3, DU145, JAC-1, TsuPr1, and LNCaP cells were treated with Andrographolide (Andro) and TRAIL, and the apoptosis was measured using the Annexin V/PI double staining method. Real time-polymerase chain reaction (PCR) and Western blot analysis were performed to measure the expression levels of target molecules...
September 1, 2017: Asian Journal of Andrology
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