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Duplex scan

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https://www.readbyqxmd.com/read/28434220/effects-of-the-backbone-and-chemical-linker-on-the-molecular-conductance-of-nucleic-acid-duplexes
#1
Edward Beall, Selma Ulku, Chaoren Liu, Emil Wierzbinski, Y Zhang, Yookyung Bae, Peng Zhang, Catalina Achim, David N Beratan, David H Waldeck
Scanning tunneling microscope-break junction measurements are used to examine how the molecular conductance of nucleic acids depends on the composition of their backbone and the linker group to the electrodes. Molecular conductances of 10-base pair long homoduplexes of DNA, aeg-PNA, γ-PNA, and a heteroduplex of DNA/aeg-PNA with identical nucleobase sequence were measured. The molecular conductance was found to vary by 12 to 13 times with the change in backbone. Computational studies show that the molecular conductance differences between nucleic acids of different backbones correlate with differences in backbone structural flexibility...
April 23, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28417852/nuclear-medicine-in-pediatric-nephro-urology-an-overview
#2
REVIEW
Iosif Mendichovszky, Bernardita Troncoso Solar, Naima Smeulders, Marina Easty, Lorenzo Biassoni
In the context of ante-natally diagnosed hydronephrosis, the vast majority of children with a dilated renal pelvis do not need any surgical treatment, as the dilatation resolves spontaneously with time. Slow drainage demonstrated at Tc-99m-mercaptoacetyltriglycine (MAG3) renography does not necessarily mean obstruction. Obstruction is defined as resistance to urinary outflow with urinary stasis at the level of the pelvic-ureteric junction (PUJ) which, if left untreated, will damage the kidney. Unfortunately this definition is retrospective and not clinically helpful...
May 2017: Seminars in Nuclear Medicine
https://www.readbyqxmd.com/read/28411703/a-novel-duplex-finding-of-superficial-epigastric-vein-flow-reversal-to-diagnose-iliocaval-occlusion
#3
Raghu Kolluri, Brian Fowler, Gary Ansel, Mitchell Silver
OBJECTIVE: Although duplex ultrasound (DUS) imaging is the current gold standard in the diagnosis of femoropopliteal deep venous thrombosis, it is not an optimal diagnostic modality to diagnose iliocaval occlusion. Screening for iliocaval occlusion thus remains a challenge for clinicians because of the lack of a reliable noninvasive technique. This challenge results in most patients undergoing computed tomography venography or magnetic resonance venography or invasive venography and intravascular ultrasound imaging...
May 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28408089/mid-term-outcomes-of-endovenous-laser-ablation-in-patients-with-active-and-healed-venous-ulcers-a-follow-up-study
#4
H Sinabulya, R Östmyren, L Blomgren
OBJECTIVES: The aim of this study was to assess the mid-term ulcer recurrence rate in patients with healed or active venous ulcers treated with endovenous laser ablation (EVLA) for incompetent superficial axial veins and to search for possible risk factors for non-healing and recurrence. METHODS: Consecutive patients treated with EVLA because of a healed or active venous ulcer between 2006 and 2013 were identified in the medical records and quality registry and invited to follow-up, including clinical history, study examination, Duplex ultrasound scanning, ankle brachial pressure, photoplethysmography, venous clinical severity score (VCSS), and health related quality of life (HRQoL) measured with EQ5D...
April 10, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28377028/transurethral-incision-of-ureterocele-does-the-time-of-presentation-affect-the-need-for-further-surgical-interventions
#5
A Hodhod, R Jednak, J Khriguian, M El-Sherbiny
OBJECTIVES: Ureterocele management is considered to be one of the famous debates in pediatric urology. Despite some considering transurethral ureterocele incision (TUI) as a temporary line of treatment, others have reported good results in terms of being a definitive treatment. The present study evaluated the feasibility of TUI as a definitive line of management. Moreover, it studied the impact of presentation on the outcomes. PATIENTS AND METHODS: The charts of patients who had ureteroceles from 1995 to 2015 were retrospectively reviewed...
March 18, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28370148/the-serial-duplex-index-improves-differential-diagnosis-of-acute-renal-transplant-dysfunction
#6
Markus Meier, Lutz Fricke, Katrin Eikenbusch, Emma Smith, Jan Kramer, Hendrik Lehnert, Martin Nitschke
OBJECTIVES: Renal duplex sonography represents a standard noninvasive diagnostic procedure to demonstrate morphologic changes in acute kidney transplant dysfunction. We investigated whether a newly developed serial duplex index (SDI) can differentiate between acute cellular rejection and acute vascular rejection more effectively than the established Doppler parameters of the resistive index (RI) and pulsatility index (PI) in recently transplanted patients. METHODS: Serial duplex scans of patients with histologically proven acute tubular necrosis (n = 25), acute cellular rejection (n = 28), acute vascular rejection (n = 18), and normal graft function (n = 50, partially protocol biopsied) were retrospectively analyzed...
April 3, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28347575/validation-of-a-basic-neurosonology-laboratory-for-detecting-cervical-carotid-artery-stenosis
#7
C de la Cruz Cosme, M S Dawid Milner, G Ojeda Burgos, A Gallardo Tur, M Márquez Martínez, T Segura
INTRODUCTION: Most of the cases of ischaemic stroke in our setting are of atherothrombotic origin. Detecting intracranial and cervical carotid artery stenosis in patients with ischaemic stroke is therefore essential. Ultrasonography has become the tool of choice for diagnosing carotid artery stenosis because it is both readily accessibility and reliable. However, use of this technique must be validated in each laboratory. The purpose of this study is to validate Doppler ultrasound in our laboratory as a means of detecting severe carotid artery stenosis...
March 24, 2017: Neurología: Publicación Oficial de la Sociedad Española de Neurología
https://www.readbyqxmd.com/read/28338457/transplant-renal-vein-thrombosis
#8
Khaled El Zorkany, Julie-Michelle Bridson, Ajay Sharma, Ahmed Halawa
Transplant renal vein thrombosis usually occurs early after surgery with a reported prevalence of 0.1% to 4.2%. It is a devastating event that ultimately leads to graft loss in almost all cases. There are many predisposing factors related to donor, recipient, surgery, and immunosuppression, with mechanical factors being considered the most common causes of transplant renal vein thrombosis. The clinical manifestations of acute renal vein thrombosis are nonspecific and are not dissimilar to the features of urine leak, urinary obstruction, or severe acute rejection...
April 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28290876/-circadian-clock-gene-variants-parameters-of-arterial-stiffness-and-blood-pressure-levels-in-subjects-without-arterial-hypertension
#9
V A Korneva, I V Kurbatova, L V Topchieva, S N Kolomeichuk, T Yu Kuznetsova, N N Nemova
CLOCK gene polymorphic variants, parameters of arterial stiffness and blood pressure (BP) variability were studied in 115 normotensive Russian patients without cardiovascular diseases (62 men, 53 women; mean age 36.4+/-1.01 years). Examination included ECG, 24h BP monitoring, duplex scan of carotid arteries, registration of vascular stiffness parameters, and genotyping of the following polymorphic markers of CLOCK: 3111T>C (3-untranslated region), 862T>C (exon 9) and 257T>G (promoter region) by PCR-RFLP method...
August 2016: Kardiologiia
https://www.readbyqxmd.com/read/28261152/comparison-between-brain-computed-tomography-scan-and-transcranial-sonography-to-evaluate-third-ventricle-width-peri-mesencephalic-cistern-and-sylvian-fissure-in-traumatic-brain-injured-patients
#10
Raphael A G Oliveira, Marcelo de Oliveira Lima, Wellingson Silva Paiva, Luiz Marcelo de Sá Malbouisson, Manuel Jacobsen Teixeira, Edson Bor-Seng-Shu
INTRODUCTION: Transcranial color-coded duplex sonography (TCCS) may help guide multimodal monitoring in the neurocritical setting. It may provide indirect information about intracranial hypertension, such as midline shift, third ventricle width, and peri-mesencephalic cistern obliteration. We aim to assess the agreement between brain computed tomography scan (CT scan) and TCCS in traumatic brain injury (TBI) patients. METHODS: In this retrospective cross-sectional observational study, TCCS was performed within 6 h before a brain CT scan...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28255445/a-failure-of-preoperative-duplex-imaging-to-diagnose-a-lower-extremity-venous-aneurysm-in-a-patient-with-severe-chronic-venous-insufficiency
#11
Roy Wesley Jones, Godfrey Ross Parkerson, Mary Ottinger, Eduardo Rodriguez, Brian Park
OBJECTIVE: We present a case of recurrent bilateral lower extremity venous stasis ulcers in association with a superficial venous aneurysm at the right saphenofemoral junction that was misdiagnosed on preoperative duplex scanning. METHODS: A 53-year-old female presented to our clinic with 6-year history of bilateral lower extremity venous stasis ulcers. Her past medical history was significant for refractory venous stasis ulcers of the bilateral lower extremities present for 6 years and morbid obesity...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/28252629/-diagnosis-and-treatment-of-benign-paroxysmal-positional-vertigo-in-common-clinical-practice
#12
N V Lebedeva, M V Zamergrad, V A Parfenov, L M Antonenko
AIM: To analyze typical medical practice in managing patients with benign paroxysmal positional vertigo (BPPV). SUBJECTS AND METHODS: 33 patients (5 men and 28 women; mean age, 58 years) with BPPV who had been referred for consultation to the Medical Diagnostic Department, Clinic of Nervous Diseases, I.M. Sechenov First Moscow State Medical University, regarding for vertigo, were examined. Information about the disease before visiting the clinic, such as the duration of vertigo, its pattern, and triggers, previously established diagnoses, prescribed treatment and its efficacy, concomitant diseases, was analyzed...
2017: Terapevticheskiĭ Arkhiv
https://www.readbyqxmd.com/read/28251966/-scanning-for-kras-nras-braf-and-pik3ca-mutations-by-dna-melting-analysis-with-taqman-probes
#13
I V Botezatu, I O Panchuk, A M Stroganova, A I Senderovich, V N Kondratova, V P Shelepov, A V Lichtenstein
Scanning for mutations by DNA melting analysis (DMA) is based on asymmetric PCR followed by the melting of duplexes formed by single-stranded amplicons with TaqMan probes. The method is optimally suited for clinical genetic testing; it is easy to perform, high-throughput, and sensitive. The detection limit of mutant alleles by the DMA method is about 3%, which is much higher than the sensitivity of Sanger sequencing. In addition, the DMA method is realized in a closed-tube format, while 2-h assay is carried out in a single tube without any intermediate or additional procedures thereby minimizing the risk of cross contamination of the samples...
January 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28214496/new-diagnostic-modalities-in-the-evaluation-of-lymphedema
#14
REVIEW
Thomas F O'Donnell, John C Rasmussen, Eva M Sevick-Muraca
OBJECTIVE: Currently, lymphedema (LED) is typically diagnosed clinically on the basis of a patient's history and characteristic physical findings. Whereas the diagnosis of LED is sometimes confirmed by lymphoscintigraphy (LSG), the technique is limited in both its ability to identify disease and to guide therapy. Recent advancements provide opportunities for new imaging techniques not only to assist in the diagnosis of LED, based on anatomic changes, but also to assess contractile function and to guide therapeutic intervention...
March 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28207510/endovascular-repair-of-bilateral-common-iliac-artery-aneurysms-using-gore-excluder-iliac-branch-endoprosthesis-without-aortobi-iliac-stent-graft-conjunction-a-case-report
#15
Vincenzo Ardita, Alessia Giaquinta, Massimiliano Veroux, Angelo Sanfiorenzo, Carla Virgilio, Giuseppe D'Arrigo, Pierfrancesco Veroux
INTRODUCTION: Bilateral common iliac artery (CIA) aneurysm (CIAA) is a rare entity. In the past decade, different endovascular approaches have been adopted for patients with several comorbidities or unfit for open repair (OR). Recently, the use of iliac branch stent graft has been proposed, resulting in satisfactory patency rates and decrease in morbidity. Currently, according to instruction for use, the iliac branch stent graft is to be used with aortobi-iliac stent graft conjunction...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28132743/editor-s-choice-factors-associated-with-long-term-outcome-in-191-patients-with-ilio-femoral-dvt-treated-with-catheter-directed-thrombolysis
#16
P Foegh, L P Jensen, L Klitfod, R Broholm, N Bækgaard
OBJECTIVE: To identify factors associated with long-term treatment success after catheter-directed thrombolysis (CDT) for acute deep venous thrombosis (DVT) involving the ilio-femoral vein. MATERIAL AND METHODS: This was a non-randomised observational cohort study. From 1999 to 2013, 191 consecutive patients (203 limbs) attending a tertiary vascular centre at Gentofte University Hospital, Denmark underwent CDT. All patients had ultrasonically verified acute ilio-femoral DVT with open distal popliteal vein and calf veins...
March 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/28056580/utility-of-adjunctive-digital-subtraction-venography-for-the-treatment-of-saphenous-vein-insufficiency
#17
Ramon L Varcoe, Shannon D Thomas, Victor Bourke, Nicole M K Rübesamen, Andrew F Lennox
PURPOSE: To report the use of adjunctive venography for the treatment of superficial venous reflux. METHODS: Two hundred consecutive patients (mean age 60.9 years, range 33-86; 128 women) with chronic venous disease underwent saphenous or perforator vein ablation in 268 limbs (305 venous trunks) guided by adjunctive venography and fluoroscopy in addition to ultrasound between October 2010 and May 2016. Intraprocedural venograms were independently evaluated by 2 vascular specialists to identify the presence of venous anomalies and the need for fluoroscopy-guided maneuvers to successfully complete venous ablation...
January 1, 2017: Journal of Endovascular Therapy
https://www.readbyqxmd.com/read/27998486/graphene-oxide-directed-in-situ-deposition-of-electroactive-silver-nanoparticles-and-its-electrochemical-sensing-application-for-dna-analysis
#18
Ningning Gao, Feng Gao, Suyu He, Qionghua Zhu, Jiafu Huang, Hidekazu Tanaka, Qingxiang Wang
The development of high-performance biosensing platform is heavily dependent on the recognition property of the sensing layer and the output intensity of the signal probe. Herein, we present a simple and highly sensitive biosensing interface for DNA detection on the basis of graphene oxide nanosheets (GONs) directed in-situ deposition of silver nanoparticles (AgNPs). The fabrication process and electrochemical properties of the biosensing interface were probed by electrochemical techniques and scanning electron microscopy...
January 25, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27998051/follow-up-and-surveillance-of-vein-grafts-when-and-how-to-intervene-to-prevent-complications
#19
REVIEW
Olivia M McBRIDE, Roderick T Chalmers
Despite advances in endovascular strategies, surgical infrainguinal lower limb revascularization remains the gold standard treatment for critical lower limb ischemia and disabling claudication. Maintaining patency of infrainguinal vein bypass grafts has been a challenging task for vascular surgeons as they are prone to developing stenoses which may precipitate failure of the bypass. Duplex ultrasound scanning has evolved as the investigation of choice to identify vein graft lesions but graft surveillance programs using this technique remain controversial...
April 2017: Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/27990113/iliofemoral-venous-thrombosis-mainly-related-to-iliofemoral-venous-obstruction-by-external-tumor-compression-in-cancer-patients
#20
Tzu-Yao Liao, Hui-Ching Hsu, Min-Sheng Wen, Yu-Hsiang Juan, Yu-Hsin Hung, Chuang-Chi Liaw
BACKGROUND: To study iliofemoral venous thrombosis related to iliofemoral venous obstruction in cancer patients. METHODS: In this case series study, 829 cancer patients were surveyed for iliofemoral obstruction/thrombosis within 10 years. The criteria for inclusion were: (1) presence of unilateral lower-extremity swelling; (2) computed tomography (CT) scans showing a tumor with external compression of the iliac or femoral vein, and (3) duplex ultrasound scans showing venous thrombosis or venous flow insufficiency over a femoral vein or saphenous vein...
September 2016: Case Reports in Oncology
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