keyword
https://read.qxmd.com/read/38525309/bioactive-coating-provides-antimicrobial-protection-through-immunomodulation-and-phage-therapeutics
#21
REVIEW
Kenny Zhuoran Wu, Zhicheng Le, Ba Myint, Brian Chan, Ling Liu, Hua Huang, Swee Leong Sing, Andy Tay
Medical implant-associated infections (IAI) is a growing threat to patients undergoing implantation surgery. IAI prevention typically relies on medical implants endowed with bactericidal properties achieved through surface modifications with antibiotics. However, the clinical efficacy of this traditional paradigm remains suboptimal, often necessitating revision surgery and posing potentially lethal consequences for patients. To bolster the existing anti-IAI arsenal, we propose herein a chitosan-based bioactive coating, i...
June 2024: Materials today. Bio
https://read.qxmd.com/read/38525082/corrigendum-whole-genome-sequencing-annotation-and-biological-characterization-of-a-novel-siphoviridae-phage-against-multi-drug-resistant-propionibacterium-acne
#22
Danxi Liao, Jian Zhang, Ruolan Liu, Kui Chen, Yuanyuan Liu, Yuming Shao, Xi Shi, Yiming Zhang, Zichen Yang
[This corrects the article DOI: 10.3389/fmicb.2022.1065386.].
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38525078/characterization-of-es10-lytic-bacteriophage-isolated-from-hospital-waste-against-multidrug-resistant-uropathogenic-e-coli
#23
JOURNAL ARTICLE
Aneela Nawaz, Sabeena Zafar, Abdulrahman H Alessa, Nauman Ahmed Khalid, Muqaddas Shahzadi, Alina Majid, Malik Badshah, Aamer Ali Shah, Samiullah Khan
Escherichia coli is the major causative agent of urinary tract infections worldwide and the emergence of multi-drug resistant determinants among clinical isolates necessitates the development of novel therapeutic agents. Lytic bacteriophages efficiently kill specific bacteria and seems promising approach in controlling infections caused by multi-drug resistant pathogens. This study aimed the isolation and detailed characterization of lytic bacteriophage designated as ES10 capable of lysing multidrug-resistant uropathogenic E...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38525040/broiler-farming-practices-using-new-or-re-used-bedding-inclusive-of-free-range-have-no-impact-on-campylobacter-levels-species-diversity-campylobacter-community-profiles-and-campylobacter-bacteriophages
#24
JOURNAL ARTICLE
Helene Nalini Chinivasagam, Wiyada Estella, Damien Finn, David G Mayer, Hugh Rodrigues, Ibrahim Diallo
A multi-stage option to address food-safety can be produced by a clearer understanding of Campylobacter 's persistence through the broiler production chain, its environmental niche and its interaction with bacteriophages. This study addressed Campylobacter levels, species, genotype, bacteriophage composition/ levels in caeca, litter, soil and carcasses across commercial broiler farming practices to inform on-farm management, including interventions. Broilers were sequentially collected as per company slaughter schedules over two-years from 17 farms, which represented four commercially adopted farming practices, prior to the final bird removal (days 39-53)...
2024: AIMS Microbiology
https://read.qxmd.com/read/38524303/solid-liquid-distribution-of-sars-cov-2-in-primary-effluent-of-a-wastewater-treatment-plant
#25
JOURNAL ARTICLE
Femi F Oloye, Yuwei Xie, Mohsen Asadi, Jonathan K Challis, Charles A Osunla, Pu Xia, Jenna Cantin, Oluwabunmi P Femi-Oloye, Markus Brinkmann, Kerry N McPhedran, Mike Sadowski, Sudhir Pandey, Paul D Jones, Chand Mangat, Mark R Servos, John P Giesy
Distributions of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and fecal viral biomarkers between solid and liquid phases of wastewater are largely unknown. Herein, distributions of SARS-CoV-2, Pepper Mild Mottle Virus (PMMoV), and F-RNA bacteriophage group II (FRNAPH-II) were determined by viral RNA RT-qPCR. Comparison of viral recovery using three conventional fractionation methods included membrane filtration, a combination of mid-speed centrifugation and membrane filtration, and high-speed centrifugation...
June 2024: MethodsX
https://read.qxmd.com/read/38523439/a-critical-review-on-detection-of-foodborne-pathogens-using-electrochemical-biosensors
#26
JOURNAL ARTICLE
Avinash V Police Patil, Ping-Feng Yang, Chiou-Ying Yang, M S Gaur, Ching-Chou Wu
An outbreak of foodborne pathogens would cause severe consequences. Detecting and diagnosing foodborne diseases is crucial for food safety, and it is increasingly important to develop fast, sensitive, and cost-effective methods for detecting foodborne pathogens. In contrast to traditional methods, such as medium-based culture, nucleic acid amplification test, and enzyme-linked immunosorbent assay, electrochemical biosensors possess the advantages of simplicity, rapidity, high sensitivity, miniaturization, and low cost, making them ideal for developing pathogen-sensing devices...
2024: Critical Reviews in Biomedical Engineering
https://read.qxmd.com/read/38522841/phage-resistance-in-klebsiella-pneumoniae-and-bidirectional-effects-impacting-antibiotic-susceptibility
#27
JOURNAL ARTICLE
Sue C Nang, Jing Lu, Heidi H Yu, Hasini Wickremasinghe, Mohammad A K Azad, Meiling Han, Jinxin Zhao, Gauri Rao, Phillip J Bergen, Tony Velkov, Norelle Sherry, David T McCarthy, Saima Aslam, Robert T Schooley, Benjamin P Howden, Jeremy J Barr, Yan Zhu, Jian Li
OBJECTIVES: Bacteriophage (phage) therapy is a promising anti-infective option to combat antimicrobial resistance. However, the clinical utilisation of phage therapy has been severely compromised by the potential emergence of phage resistance. While certain phage resistance mechanisms can restore bacterial susceptibility to certain antibiotics, a lack of knowledge of phage resistance mechanisms hinders optimal use of phages and their combination with antibiotics. METHODS: Genome-wide transposon screening was performed with a mutant library of Klebsiella pneumoniae MKP103 to identify phage pKMKP103_1-resistant mutants...
March 22, 2024: Clinical Microbiology and Infection
https://read.qxmd.com/read/38522833/development-and-mouse-model-evaluation-of-a-new-phage-cocktail-intended-as-an-alternative-to-antibiotics-for-treatment-of-staphylococcus-aureus-induced-bovine-mastitis
#28
JOURNAL ARTICLE
Mengting Guo, Yumin Zhang, Lifei Wu, Yangjing Xiong, Lu Xia, Yuqiang Cheng, Jingjiao Ma, Hengan Wang, Jianhe Sun, Zhaofei Wang, Yaxian Yan
Bovine mastitis (BM) is a prevalent infectious disease in dairy herds worldwide, resulting in substantial economic losses. Staphylococcus aureus is a major cause of mastitis in animals, and its antibiotic resistance poses challenges for treatment. Recently, there has been a renewed interest in the development of alternative methods to antibiotic therapy, including bacteriophages (phages), for controlling bacterial infections. In this study, 2 lytic phages (designated as JDYN for vB_SauM_JDYN and JDF86 for vB_SauM_JDF86) were isolated from the cattle sewage effluent samples collected from dairy farms in Shanghai...
March 22, 2024: Journal of Dairy Science
https://read.qxmd.com/read/38521417/impact-of-different-hand-drying-methods-on-surrounding-environment-aerosolization-of-virus-and-bacteria-and-transference-to-surfaces
#29
JOURNAL ARTICLE
Rodolphe C Hervé, Catherine Bryant, Lucy Sutton, Christian Cox, Maria Salomé Gião, Charles William Keevil, Sandra A Wilks
BACKGROUND: In recent years, hand drying has been highlighted as a key step in appropriate hand hygiene (WHO 2009), as moisture on hands can increase transference of microorganisms from hands to surfaces and vice-versa. AIM: To understand bacterial and viral aerosolization following hand drying and study transference of microorganisms from hands to surfaces after drying using different methods. METHODS: Groups of five volunteers had their hands pre-washed with soap, rinsed and dried then inoculated with a concentrated mixture of Pseudomonas fluorescens and MS2 bacteriophage...
March 21, 2024: Journal of Hospital Infection
https://read.qxmd.com/read/38520838/isolation-characterization-and-application-of-noble-bacteriophages-targeting-potato-common-scab-pathogen-streptomyces-stelliscabiei
#30
JOURNAL ARTICLE
Shihe Zhang, Cheligeer Zhang, Jian Wu, Simiao Liu, Ruofang Zhang, Utpal Handique
Bacteriophages have emerged as promising alternatives to pesticides for controlling bacterial pathogens in crops. Among these pathogens, Streptomyces stelliscabiei (syn. S. stelliscabiei) is a primary causative agent of potato common scab (PCS), resulting in substantial global economic losses. The traditional management methods for PCS face numerous challenges, highlighting the need for effective and environmentally friendly control strategies. In this study, we successfully isolated three novel bacteriophages, namely Psst1, Psst2, and Psst4, which exhibited a broad host range encompassing seven S...
March 20, 2024: Microbiological Research
https://read.qxmd.com/read/38520090/efficient-scar-free-knock-ins-of-several-kilobases-in-plants-by-engineered-crispr-cas-endonucleases
#31
JOURNAL ARTICLE
Tom Schreiber, Anja Prange, Petra Schäfer, Thomas Iwen, Ramona Grützner, Sylvestre Marillonnet, Aurélie Lepage, Maire Javelle, Wyatt Paul, Alain Tissier
In plants and mammals, non-homologous end-joining is the dominant pathway to repair DNA double strand breaks, making it challenging to generate knock-in events. We identified two groups of exonucleases from the Herpes Virus and the bacteriophage T7 families that conferred an up to 38-fold increase in HDR frequencies when fused to Cas9/Cas12a in a Tobacco mosaic virus-based transient assay in Nicotiana benthamiana. We achieved precise and scar-free insertion of several kilobases of DNA both in transient and stable transformation systems...
March 22, 2024: Molecular Plant
https://read.qxmd.com/read/38519791/bacteriophages-and-host-inflammation-in-ibd
#32
JOURNAL ARTICLE
Eleni Kotsiliti
No abstract text is available yet for this article.
March 22, 2024: Nature Reviews. Gastroenterology & Hepatology
https://read.qxmd.com/read/38517580/preclinical-characterization-and%C3%A2-in-silico-safety-assessment-of-three-virulent-bacteriophages-targeting-carbapenem-resistant-uropathogenic-escherichia-coli
#33
JOURNAL ARTICLE
Gunaraj Dhungana, Roshan Nepal, Ghais Houtak, George Bouras, Sarah Vreugde, Rajani Malla
Phage therapy has recently been revitalized in the West with many successful applications against multi-drug-resistant bacterial infections. However, the lack of geographically diverse bacteriophage (phage) genomes has constrained our understanding of phage diversity and its genetics underpinning host specificity, lytic capability, and phage-bacteria co-evolution. This study aims to locally isolate virulent phages against uropathogenic Escherichia coli (E. coli) and study its phenotypic and genomic features...
March 22, 2024: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://read.qxmd.com/read/38517357/using-a-human-colonoid-derived-monolayer-to-study-bacteriophage-translocation
#34
JOURNAL ARTICLE
Huu Thanh Le, Alicia Fajardo Lubian, Bethany Bowring, David van der Poorten, Jonathan Iredell, Jacob George, Carola Venturini, Golo Ahlenstiel, Scott Read
Bacteriophages (phages) are estimated to be the most abundant microorganisms on Earth. Their presence in human blood suggests that they can translocate from non-sterile sites such as the gastrointestinal tract where they are concentrated. To examine phage translocation ex vivo , we adapted a primary colonoid monolayer model possessing cell diversity and architecture, and a thick layer of mucus akin to the colonic environment in vivo . We show that the colonoid monolayer is superior to the Caco-2 cell-line model, possessing intact and organized tight junctions and generating a physiologically relevant mucus layer...
2024: Gut Microbes
https://read.qxmd.com/read/38516182/harnessing-a-t1-phage-derived-spanin-for-developing-phage-based-antimicrobial-development
#35
JOURNAL ARTICLE
Wakana Yamashita, Shinjiro Ojima, Azumi Tamura, Aa Haeruman Azam, Kohei Kondo, Zhang Yuancheng, Longzhu Cui, Masaki Shintani, Masato Suzuki, Yoshimasa Takahashi, Koichi Watashi, Satoshi Tsuneda, Kotaro Kiga
The global increase in the prevalence of drug-resistant bacteria has necessitated the development of alternative treatments that do not rely on conventional antimicrobial agents. Using bacteriophage-derived lytic enzymes in antibacterial therapy shows promise; however, a thorough comparison and evaluation of their bactericidal efficacy are lacking. This study aimed to compare and investigate the bactericidal activity and spectrum of such lytic enzymes, with the goal of harnessing them for antibacterial therapy...
2024: Biodes Res
https://read.qxmd.com/read/38513899/structural-and-functional-basis-of-bacteriophage-k64-orf41-depolymerase-for-capsular-polysaccharide-degradation-of-klebsiella-pneumoniae-k64
#36
JOURNAL ARTICLE
Tianyun Huang, Zhuoyuan Zhang, Xin Tao, Xinyu Shi, Peng Lin, Dan Liao, Chenyu Ma, Xinle Cai, Wei Lin, Xiaofan Jiang, Peng Luo, Shan Wu, Yuan Xie
Capsule polysaccharide is an important virulence factor of Klebsiella pneumoniae (K. pneumoniae), which protects bacteria against the host immune response. A promising therapeutic approach is using phage-derived depolymerases to degrade the capsular polysaccharide and expose and sensitize the bacteria to the host immune system. Here we determined the cryo-electron microscopy (cryo-EM) structures of a bacteriophage tail-spike protein against K. pneumoniae K64, ORF41 (K64-ORF41) and ORF41 in EDTA condition (K64-ORF41EDTA ), at 2...
March 20, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38511948/broad-host-range-phages-target-global-clostridium-perfringens-bacterial-strains-and-clear-infection-in-five-strain-model-systems
#37
JOURNAL ARTICLE
Anisha M Thanki, Emmanuel K Osei, Natasha Whenham, Michael G Salter, Mike R Bedford, Helen V Masey O'Neill, Martha R J Clokie
UNLABELLED: Clostridium perfringens is a prevalent bacterial pathogen in poultry, and due to the spread of antimicrobial resistance, alternative treatments are needed to prevent and treat infection. Bacteriophages (phages), viruses that kill bacteria, offer a viable option and can be used therapeutically to treat C. perfringens infections. The aim of this study was to isolate phages against C. perfringens strains currently circulating on farms across the world and establish their virulence and development potential using host range screening, virulence assays, and larva infection studies...
March 21, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38511257/gene-transfer-agents-the-ambiguous-role-of-selfless-viruses-in-genetic-exchange-and-bacterial-evolution
#38
JOURNAL ARTICLE
Paul Christopher Michael Fogg
Gene transfer agents (GTAs) are genetic elements derived from ancestral bacteriophages that have become domesticated by the host. GTAs are present in diverse prokaryotic organisms, where they can facilitate horizontal gene transfer under certain conditions. Unlike typical bacteriophages, GTAs do not exhibit any preference for the replication or transfer of the genes encoding them; instead, they exhibit a remarkable capacity to package chromosomal, and sometimes extrachromosomal, DNA into virus-like capsids and disseminate it to neighboring cells...
March 21, 2024: Molecular Microbiology
https://read.qxmd.com/read/38508303/c-terminal-domain-of-t4-gene-32-protein-enables-rapid-filament-reorganization-and-dissociation
#39
JOURNAL ARTICLE
Ben A Cashen, Michael Morse, Ioulia Rouzina, Richard L Karpel, Mark C Williams
Bacteriophage T4 gene 32 protein (gp32) is a single-stranded DNA (ssDNA) binding protein essential for DNA replication. gp32 forms stable protein filaments on ssDNA through cooperative interactions between its core and N-terminal domain. gp32's C-terminal domain (CTD) is believed to primarily help coordinate DNA replication via direct interactions with constituents of the replisome. However, the exact mechanisms of these interactions are not known, and it is unclear how tightly-bound gp32 filaments are readily displaced from ssDNA as required for genomic processing...
March 18, 2024: Journal of Molecular Biology
https://read.qxmd.com/read/38504586/a-retrospective-cohort-study-on-early-antibiotic-use-in-vaccinated-and-unvaccinated-covid-19-patients
#40
JOURNAL ARTICLE
Carlo Brogna, Luigi Montano, Maria Elisabetta Zanolin, Domenico Rocco Bisaccia, Gianluca Ciammetti, Valentina Viduto, Mark Fabrowski, Abdul M Baig, Joachim Gerlach, Iapicca Gennaro, Elio Bignardi, Barbara Brogna, Aquilino Frongillo, Simone Cristoni, Marina Piscopo
The bacteriophage behavior of SARS-CoV-2 during the acute and post-COVID-19 phases appears to be an important factor in the development of the disease. The early use of antibiotics seems to be crucial to inhibit disease progression-to prevent viral replication in the gut microbiome, and control toxicological production from the human microbiome. To study the impact of specific antibiotics on recovery from COVID-19 and long COVID (LC) taking into account: vaccination status, comorbidities, SARS-CoV-2 wave, time of initiation of antibiotic therapy and concomitant use of corticosteroids and nonsteroidal anti-inflammatory drugs (NSAIDs)...
March 2024: Journal of Medical Virology
keyword
keyword
60952
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.