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Immunophenotyping by flow cytometry

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https://www.readbyqxmd.com/read/28447425/ten-color-15-antibody-flow-cytometry-panel-for-immunophenotyping-of-lymphocyte-population
#1
REVIEW
A Rajab, O Axler, J Leung, M Wozniak, A Porwit
We have developed a lymphoproliferative disorder screening tube (LPD-ST) with the aim to provide comprehensive immunophenotyping of lymphocyte subsets with minimal need for additional testing. The LPD-ST consists of CD4/kappa FITC, CD8/lambda PE, CD3/CD14ECD, CD38PC5.5, CD20/CD56PC7, CD10APC, CD19APC-A700, CD5APC-A750, CD57/CD23PB and CD45KO. The LPD-ST was validated against previously used lymphocyte subset panels in Canada (n=60) and in Sweden (n=43) and against the OneFlow(™) LST (n=60). The LPD-ST panel was then implemented in clinical practice using dried monoclonal antibody reagents (Duraclone(®) ) on 649 patient samples in Sweden...
May 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28447408/flow-cytometry-recognizing-unusual-populations-in-leukemia-and-lymphoma-diagnosis
#2
REVIEW
M Keeney, B D Hedley, I H Chin-Yee
Flow cytometry is an invaluable technology in the examination of blood, bone marrow, tissue and body fluids for the presence or absence of hematological disease. It is used in both diagnostic and follow-up testing, with an increasingly important role in the detection of very small residual disease populations (Minimal Residual Disease, MRD) However, flow cytometry immunophenotyping of leukemia and lymphoma is highly dependent on interpretation of results and with the increased complexity of 8-10 color instruments routinely used in clinical laboratories, knowledge of disease-defining populations is increasingly important as is recognizing normal and reactive patterns...
May 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28446296/-expression-of-cc-chemokine-receptor-7-in-patiens-with-multiple-myeloma-and-its-relationship-with-extramedullary-disease
#3
Ya-Nan Wang, Si-Lin Gan, Fei-Fei Wu, Wei-Min Wang, Shuai-Quan Li, Jie Ma, Rong Guo, Zhong-Xing Jiang, Bao-Hong Yue, Hui Sun
OBJECTIVE: To investigate the expression of CC-chemokine receptor 7(CCR7) in patients with multiple myeloma(MM) and its correlation with clinical features of MM. METHODS: The level of CCR7 expression in bone marrow samples from 53 newly diagnosed MM patients was detected by flow cytometry(FCM). Statistical methods were used to analyze the correlation between CCR7 expression and clinical features, such as sex, age, M protein, peripheral blood cell count, biochemical indicators, plasma cell ratio of bone marrow, immunophenotype, osteopathy and extramedullary disease...
April 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28445424/the-effect-of-deoxynivalenol-on-selected-populations-of-immunocompetent-cells-in-porcine-blood-a-preliminary-study
#4
Michał Dąbrowski, Ewa Jakimiuk, Mirosław Baranowski, Magdalena Gajęcka, Łukasz Zielonka, Maciej Tadeusz Gajęcki
Deoxynivalenol (DON) is one of the most prevalent mycotoxins in Europe. Pigs are an animal species that is most susceptible to this mycotoxin. Deoxynivalenol causes significant losses in pig production by lowering feed intake, decreasing daily weight gains, disrupting immune responses, and increasing susceptibility to diseases. The aim of this experiment was to determine the influence of feed contaminated with DON at concentrations insignificantly higher than recommended by the European Commission (900 µg/kg)...
April 26, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28438482/manufacture-and-preparation-of-human-placenta-derived-mesenchymal-stromal-cells-for-local-tissue-delivery
#5
Lee Lankford, Y Julia Chen, Zoe Saenz, Priyadarsini Kumar, Connor Long, Diana Farmer, Aijun Wang
BACKGROUND: In this study we describe the development of a Current Good Manufacturing Practice (CGMP)-compliant process to isolate, expand and bank placenta-derived mesenchymal stromal cells (PMSCs) for use as stem cell therapy. We characterize the viability, proliferation and neuroprotective secretory profile of PMSCs seeded on clinical-grade porcine small intestine submucosa extracellular matrix (SIS-ECM; Cook Biotech). METHODS: PMSCs were isolated from early gestation placenta chorionic villus tissue via explant culture...
April 21, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28429508/immunophenotyping-and-activation-status-of-maternal-peripheral-blood-leukocytes-during-pregnancy-and-labour-both-term-and-preterm
#6
Jianhong Zhang, Oksana Shynlova, Sally Sabra, Annie Bang, Laurent Briollais, Stephen J Lye
The onset of labour in rodents and in humans is associated with physiological inflammation which is manifested by infiltration of activated maternal peripheral leukocytes (mPLs) into uterine tissues. Here, we used flow cytometry to immunophenotype mPLs throughout gestation and labour, both term and preterm. Peripheral blood was collected from non-pregnant women and pregnant women in the 1st, 2nd and 3rd trimesters. Samples were also collected from women in active labour at term (TL) or preterm (PTL) and compared with women term not-in-labour (TNIL) and preterm not-in-labour (PTNIL)...
April 21, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28428879/a-systems-biology-approach-to-investigating-the-influence-of-exercise-and-fitness-on-the-composition-of-leukocytes-in-peripheral-blood
#7
Michael P Gustafson, Ara Celi DiCostanzo, Courtney M Wheatley, Chul-Ho Kim, Svetlana Bornschlegl, Dennis A Gastineau, Bruce D Johnson, Allan B Dietz
BACKGROUND: Exercise immunology has become a growing field in the past 20 years, with an emphasis on understanding how different forms of exercise affect immune function. Mechanistic studies are beginning to shed light on how exercise may impair the development of cancer or be used to augment cancer treatment. The beneficial effects of exercise on the immune system may be exploited to improve patient responses to cancer immunotherapy. METHODS: We investigated the effects of acute exercise on the composition of peripheral blood leukocytes over time in a male population of varying fitness...
2017: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/28427520/lymphoblastic-lymphoma
#8
REVIEW
Sergio Cortelazzo, Andrés Ferreri, Dieter Hoelzer, Maurilio Ponzoni
Lymphoblastic lymphoma (LBL) is a neoplasm of immature B cells committed to the B-(B-LBL) or T-cell lineage (T-LBL) that accounts for approximately 2% of all lymphomas. Although histological features are usually sufficient to distinguish lymphoblastic from mature B- or T-cell neoplasms, of greater importance for diagnosis is the characterization of immunophenotype by flow cytometry. LBL occurs more commonly in children than in adults, mostly in males. A bone marrow involvement <25% (or 20% according to WHO) formally distinguishes LBL from ALL...
May 2017: Critical Reviews in Oncology/hematology
https://www.readbyqxmd.com/read/28424276/thrombin-induced-podocyte-injury-is-protease-activated-receptor-dependent
#9
Ruchika Sharma, Amanda P Waller, Shipra Agrawal, Katelyn J Wolfgang, Hiep Luu, Khurrum Shahzad, Berend Isermann, William E Smoyer, Marvin T Nieman, Bryce A Kerlin
Nephrotic syndrome is characterized by massive proteinuria and injury of specialized glomerular epithelial cells called podocytes. Studies have shown that, whereas low-concentration thrombin may be cytoprotective, higher thrombin concentrations may contribute to podocyte injury. We and others have demonstrated that ex vivo plasma thrombin generation is enhanced during nephrosis, suggesting that thrombin may contribute to nephrotic progression. Moreover, nonspecific thrombin inhibition has been shown to decrease proteinuria in nephrotic animal models...
April 19, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28422443/role-of-cd200-in-differential-diagnosis-of-mature-b-cell-neoplasm
#10
K Rahman, P Kumar, R Gupta, M K Singh, S Nityanand
INTRODUCTION: CD 200 is a type I immunoglobulin super family membrane glycoprotein, which is expressed in various mature B-cell neoplasm (MBN). This study aimed at analyzing the expression pattern of CD200 by flow cytometry immunophenotyping (FCI) and to evaluate its utility in narrowing down the differential diagnosis of MBN, particularly in low-grade lymphomas. METHODS: A total of 160 samples were evaluated by FCI over a period of 2 years (July 2014-June 2016), by a panel of antibodies including CD200...
April 19, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28421369/immunophenotypic-profiles-for-distinguishing-orbital-mucosa-associated-lymphoid-tissue-lymphoma-from-benign-lymphoproliferative-tumors
#11
Shunichiro Ueda, Yoshihiko Usui, Takeshi Nagai, Daniel Diaz-Aguilar, Toshitaka Nagao, Hiroshi Goto
PURPOSE: To evaluate novel immunophenotypic profiles of patients with orbital lymphoproliferative tumors. METHODS: From 2006 to 2014, surgical biopsies from consecutive patients with newly diagnosed orbital mucosa-associated lymphoid tissue (MALT) lymphoma and IgG4-related ophthalmic disease (IgG4-ROD) were collected from Tokyo Medical University Hospital. A total of 44 tumors from 44 patients were analyzed, including 21 with orbital MALT lymphoma (11 men and 10 women, mean age 67...
April 18, 2017: Japanese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28418282/immunohistochemical-detection-of-hairy-cell-leukemia-in-paraffin-sections-the-role-of-pax5-and-cd103-double-staining-to-improve-specificity-and-sensitivity
#12
Igor B Rozenvald, Maurice D Richardson, Lenessa Brock, Russell L Maiese
CONTEXT: - In hematopathology practice, abnormal expression of CD103 on B cells is detected by flow cytometry in hairy cell leukemia (HCL) and, in combination with other phenotypic and morphologic findings, provides diagnostic specificity and sensitivity. Immunostaining on paraffin sections makes it possible to perform immunophenotyping in situ. However, normal bone marrow contains CD103-positive cells, which are not B cells, making it difficult to be certain about low-level involvement by HCL...
April 18, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28414589/progesterone-decreases-in-vitro-indoleamine-2-3-dioxygenase-expression-in-dendritic-and-cd4-cells-from-maternal-fetal-interface-of-rats
#13
Pedro Kastein Faria da Cunha Bianchi, Rafael Magdanelo Leandro, Aline Nayara Poscai, Tulio Yoshinaga, Patrícia Orlandini Gonçalez, José Roberto Kfoury Junior
PROBLEM: Several mechanisms contribute to the tolerogenic state observed during pregnancy, such as the activity of the enzyme indoleamine 2, 3-dioxygenase (IDO). This initializes the catabolism of tryptophan, inducing T cells to apoptosis due to its deprivation and by the action of its catabolites in the placental microenvironment. Progesterone plays an important part on immunological tolerance mechanisms during pregnancy; however, there is no evidence it is related to IDO activity. Thus, this study aimed to investigate progesterone influence on the maternal-fetal interface of pregnant Wistar rats, by identifying IDO positive cells by immunophenotyping and flow cytometry under exogenous progesterone supplementation...
April 17, 2017: Immunological Investigations
https://www.readbyqxmd.com/read/28408205/temporal-spatial-and-phenotypical-changes-of-pdgfr%C3%AE-expressing-fibroblasts-during-late-lung-development
#14
Mehari Endale, Shawn Ahlfeld, Erik Bao, Xiaoting Chen, Jenna Green, Zach Bess, Matthew T Weirauch, Yan Xu, Anne Karina Perl
Many studies have investigated the source and role of epithelial progenitors during lung development; such information is limited for fibroblast populations and their complex role in the developing lung. In this study, we characterized the spatial location, mRNA expression and Immunophenotyping of PDGFRα(+) fibroblasts during sacculation and alveolarization. Confocal microscopy identified spatial association of PDGFRα expressing fibroblasts with proximal epithelial cells of the branching bronchioles and the dilating acinar tubules at E16...
April 11, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28404628/simultaneous-detection-of-decidual-th1-th2-and-nk1-nk2-immunophenotyping-in-unknown-recurrent-miscarriage-using-8-color-flow-cytometry-with-fsc-vt-extended-strategy
#15
Peng Dong, Xi Wen, Jia Liu, Cui-Yan Yan, Jing Yuan, Lan-Rong Luo, Qiao-Fei Hu, Jian Li
Th1/Th2 imbalance is considered as a mechanism of recurrent miscarriage. The NK1/NK2 paradigm is hypothesized to play an important role in pregnancy. However, few results showed simultaneous changes of these subsets in vivo in decidual tissues. This study aimed to detect the decidual mononuclear cells and the Th1/Th2 and NK1/NK2 paradigm simultaneously using multiparametric flow cytometry (MFC) in unexplained recurrent miscarriages (URM). Mononuclear cells were isolated from the decidual tissues of URM cases and early pregnant women...
April 12, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28403936/the-short-term-and-long-term-effects-of-bariatric-metabolic-surgery-on-subcutaneous-adipose-tissue-inflammation-in-humans
#16
Derek K Hagman, Ilona Larson, Jessica N Kuzma, Gail Cromer, Karen Makar, Katya B Rubinow, Karen E Foster-Schubert, Brian van Yserloo, Peter S Billing, Robert W Landerholm, Matthew Crouthamel, David R Flum, David E Cummings, Mario Kratz
CONTEXT: The mechanisms mediating the short- and long-term improvements in glucose homeostasis following bariatric/metabolic surgery remain incompletely understood. OBJECTIVE: To investigate whether a reduction in adipose tissue inflammation plays a role in the metabolic improvements seen after bariatric/metabolic surgery, both in the short-term and longer-term. DESIGN: Fasting blood and subcutaneous abdominal adipose tissue were obtained before (n=14), at one month (n=9), and 6-12months (n=14) after bariatric/metabolic surgery from individuals with obesity who were not on insulin or anti-diabetes medication...
May 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28399903/overexpression-of-syndecan-1-muc-1-and-putative-stem-cell-markers-in-breast-cancer-leptomeningeal-metastasis-a-cerebrospinal-fluid-flow-cytometry-study
#17
Iole Cordone, Serena Masi, Valentina Summa, Mariantonia Carosi, Antonello Vidiri, Alessandra Fabi, Alessia Pasquale, Laura Conti, Immacolata Rosito, Carmine Maria Carapella, Veronica Villani, Andrea Pace
BACKGROUND: Cancer is a mosaic of tumor cell subpopulations, where only a minority is responsible for disease recurrence and cancer invasiveness. We focused on one of the most aggressive circulating tumor cells (CTCs) which, from the primitive tumor, spreads to the central nervous system (CNS), evaluating the expression of prognostic and putative cancer stem cell markers in breast cancer (BC) leptomeningeal metastasis (LM). METHODS: Flow cytometry immunophenotypic analysis of cerebrospinal fluid (CSF) samples (4...
April 11, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28390393/regulatory-monocytes-in-helminth-infections-insights-from-the-modulation-during-human-hookworm-infection
#18
Lívia Silva Araújo Passos, Pedro Henrique Gazzinelli-Guimarães, Tiago Antônio de Oliveira Mendes, Ana Clara Gazzinelli Guimarães, Denise da Silveira Lemos, Natasha Delaqua Ricci, Ricardo Gonçalves, Daniella Castanheira Bartholomeu, Ricardo Toshio Fujiwara, Lilian Lacerda Bueno
BACKGROUND: While the macrophage polarization is well characterized in helminth infections, the natural heterogeneity of monocytes with multiple cell phenotypes might influence the outcome of neglected diseases, such hookworm infection. Here, we report the profile of monocytes in human hookworm infections as a model to study the regulatory subpopulation of monocytes in helminth infections. METHODS: Blood samples were collected from 19 Necator americanus-infected individuals and 13 healthy individuals...
April 8, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28385331/generalized-verrucosis-and-abnormal-t-cell-activation-due-to-homozygous-taok2-mutation
#19
Vered Molho-Pessach, Yuval Ramot, Maxim Mogilevsky, Leonor Cohen-Daniel, Eli M Eisenstein, Abdulsalam Abu-Libdeh, Ihab Siam, Michael Berger, Rotem Karni, Abraham Zlotogorski
BACKGROUND: Generalized verrucosis (GV) is a chronic and progressive cutaneous human papillomavirus (HPV) infection resulting in multiple warts and associated with acquired or genetic immune defects. We identified a consanguineous Arab family manifesting GV and recurrent bacterial and viral infections, in association with inflammatory bowel disease (IBD). OBJECTIVE: To identify the mutated gene responsible for GV, recurrent infections and IBD, in this family. METHODS: Flow cytometry of peripheral blood mononuclear cells was performed, as well as proliferation and cell cycle assays of T cells...
March 27, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28380441/clinical-and-prognostic-significance-of-aberrant-t-cell-marker-expression-in-225-cases-of-de-novo-diffuse-large-b-cell-lymphoma-and-276-cases-of-other-b-cell-lymphomas
#20
Naoko Tsuyama, Daisuke Ennishi, Masahiro Yokoyama, Satoko Baba, Reimi Asaka, Yuko Mishima, Yasuhito Terui, Kiyohiko Hatake, Kengo Takeuchi
Expression of T-cell markers, generally investigated for immunophenotyping of T-cell lymphomas, is also observed in several types of B-cell lymphomas, including diffuse large B-cell lymphoma (DLBCL). We previously reported that CD5 expression in DLBCL is an inferior prognostic factor in the era of rituximab. However, data regarding the frequencies, histological relevance, and prognostic importance of T-cell markers other than CD5 are currently unavailable. In the present study, we comprehensively evaluated the expression of T-cell markers (CD2, CD3, CD4, CD5, CD7, and CD8) in 501 B-cell lymphomas, including 225 DLBCLs, by flow cytometry and subsequent immunohistochemistry...
March 23, 2017: Oncotarget
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