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Cassandra Rauert, Mahiba Shoieb, Jasmin K Schuster, Anita Eng, Tom Harner
Poly- and per-fluoroalkyl substances (PFAS) and volatile methyl siloxanes (VMS) were monitored at 21 sites in the Global Atmospheric Passive Sampling (GAPS) Network. Atmospheric concentrations previously reported from 2009 were compared to concentrations measured at these sites in 2013 and 2015, to assess trends over 7 years of monitoring. Concentrations of the fluorotelomer alcohols (FTOHs) and fluorinated sulfonamides and sulfonamidoethanols (FOSAs and FOSEs) were stable at these sites from 2009 to 2015 with no significant difference (p > 0...
March 13, 2018: Environmental Pollution
Si-Yuan Shao, Ya-Nan Yang, Zi-Ming Feng, Jian-Shuang Jiang, Pei-Cheng Zhang
An efficient1 H NMR spectroscopic approach for determining the relative configurations of lignans with a 7,9':7',9-diepoxy moiety has been established. Using the chemical shift differences of H2 -9 and H2 -9' (ΔδH-9 and ΔδH-9' ), the configurations of 8-H and 8-OH furofuran lignans can be rapidly and conveniently determined. The rule is applicable for data acquired in DMSO- d6 , methanol- d4 , or CDCl3 . Notably, the rule should be applied carefully when the C-2 or C-6 substituent of the aromatic rings may alter the dominant conformers of the furofuran moiety...
March 14, 2018: Journal of Natural Products
E C Welch, S Ya Tochitsky, J J Pigeon, C Joshi
A new method for a long-wave infrared (LWIR), picosecond difference frequency generation (DFG) source using one near-infrared laser and a Raman shifter is experimentally tested and characterized. The signal seed for DFG is a Stokes pulse generated via transient stimulated Raman scattering in a nonlinear medium with a Raman frequency in the 2-20 µm range. A study of the dynamics of the transient Raman regime in liquid C6 D6 has shown that the efficiency of Stokes production can be increased and the central wavelength can be controlled by chirping the pump pulse in order to compensate for chirping caused by self-phase modulation...
March 5, 2018: Optics Express
Lucas Primo de Carvalho Alves, Neusa Sica da Rocha
OBJECTIVES: Melancholic depression is a type of depression which is closely related to biological variables than are other types of depression. Its clinical features can be assessed using six items on the Hamilton Depression Rating Scale (HAM-D6 ). Previous studies have shown, using item response theory, that the symptom depressed mood is the least severe melancholic feature; work and activities, somatic symptoms and psychic anxiety are of moderate severity; and feelings of guilt and psychomotor retardation are the most severe...
March 8, 2018: Bipolar Disorders
Hye-Nan Park, Young-Jun Lim, Won-Jin Yi, Jung-Suk Han, Seung-Pyo Lee
PURPOSE: The aim of this study was to design an intraoral environment simulator and to assess the accuracy of two intraoral scanners using the simulator. MATERIALS AND METHODS: A box-shaped intraoral environment simulator was designed to simulate two specific intraoral environments. The cast was scanned 10 times by Identica Blue (MEDIT, Seoul, South Korea), TRIOS (3Shape, Copenhagen, Denmark), and CS3500 (Carestream Dental, Georgia, USA) scanners in the two simulated groups...
February 2018: Journal of Advanced Prosthodontics
Reyes Niradiz, Benedetti Ines, Rebollo Juan, Correa Oscar, Geliebter Jan
Atypical chemokine receptors have recently emerged as important molecular players in health and diseases; they affect chemokine availability and function and impact a multitude of pathophysiological events, including the tumorigenesis process. This family of atypical receptors comprises five members: ACKR1/DARC, ACKR2/D6, ACKR3/CXCR7, ACKR4/CCRL1, and ACKR5/CCRL2. This work evaluated the differential expression of these receptors in prostate cancer using quantitative PCR. Further evaluation of CCRL2 at the protein level confirmed its overexpression in a metastatic cell line and in malignant prostatic tissues from patients...
November 1, 2017: Journal of Biomedical Research
Ken Haga, Lena Frank, Taro Kimura, Claus Schwechheimer, Tatsuya Sakai
Regulation of protein function by phosphorylation and dephosphorylation are major regulatory mechanisms in many cellular events. The phototropin blue-light photoreceptors, plant-specific AGCVIII kinases, are essential for phototropic responses. Members of the D6 PROTEIN KINASE (D6PK) family, representing a subfamily of the AGCVIII kinases, also contribute to phototropic responses, suggesting that possibly further AGCVIII kinases may potentially control phototropism. The present study investigates the functional roles of Arabidopsis (Arabidopsis thaliana) AGCVIII kinases in hypocotyl phototropism...
February 26, 2018: Plant & Cell Physiology
Sonja Schelhaas, Lydia Wachsmuth, Sven Hermann, Natascha Rieder, Astrid Heller, Kathrin Heinzmann, Davina J Honess, Donna-Michelle Smith, Inga B Fricke, Nathalie Just, Sabrina Doblas, Ralph Sinkus, Christian Döring, Klaus Peter Schäfers, John R Griffiths, Cornelius Faber, Richard Schneider, Eric Ofori Aboagye, Andreas Hans Jacobs
Non-invasive monitoring of tumor therapy response helps in developing personalized treatment strategies. Here, we performed sequential positron emission tomography (PET) and diffusion-weighted magnetic resonance imaging (DW-MRI) to evaluate changes induced by a FOLFOX-like combination chemotherapy in colorectal cancer (CRC) xenografts, to identify the cellular and molecular determinants of these imaging biomarkers. Methods: Tumor bearing CD1 nude mice, engrafted with FOLFOX-sensitive Colo205 CRC xenografts, were treated with FOLFOX (5 fluorouracil, leucovorin and oxaliplatin) in weekly intervals...
February 23, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Berit P Jensen, Rajneeta Saraf, Jing Ma, Sarah Berry, Cameron C Grant, Carlos A Camargo, Christiaan W Sies
BACKGROUND: Demand for measurement of 25-hydroxyvitamin D (25OHD) is growing and dried blood spot (DBS) sampling is attractive as samples are easier to collect, transport and store. METHODS: A 2D LC-MS/MS assay without derivatization was developed. DBS punches (3.2 mm) were ultrasonicated with d6 -25OHD3 in 70% methanol followed by hexane extraction, dry-down and reconstitution. The assay was validated and applied to two studies comparing whole blood adult DBS with serum samples (n = 40) and neonatal whole blood DBS with cord serum samples (n = 80)...
February 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Gorka Zabalo, Daniel de Frutos, Juan Carlos García, Rodrigo Ortega, Juan José Guelbenzu, Idoya Zazpe
We report a case of a 41 years old patient complaining of chronic dorsalgia. MRI showed a well defined intradural extramedular dumbbell-shaped lesion, associated to a left paravertebral tumor at D5-D6 level. The tumor was embolizated prior to surgery. Following she underwent a D4-D6 laminotomy, left D5-D6 costotransversectomy and resection of the intracanal extradural part of the lesion with section of the left D5 nerve. Posteriorly, complete resection of the extracanal portion of the tumor was performed by a postero-lateral approach...
February 19, 2018: Neurocirugía
Yue Zhang, Mingyun Ni, Na Liu, Yongjiang Zhou, Xuemei Chen, Yu-Bin Ding, Junlin He, Yingxiong Wang, Xueqing Liu, Yanqing Geng, Liling Xie
Embryo implantation is a complex process involving synchronised crosstalk between a receptive endometrium and functional blastocysts. Apoptosis plays an important role in this process as well as in the maintenance of pregnancy. In this study, we analysed the expression pattern of programmed cell death 4 (Pdcd4), a gene associated with apoptosis in the mouse endometrium, during early pregnancy and pseudopregnancy by real-time quantitative polymerase chain reaction, in situ hybridisation, western blotting and immunohistochemistry...
February 19, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Maruthi Konda, Rohit G Jadhav, Sayan Maiti, Shaikh M Mobin, Brice Kauffmann, Apurba K Das
Constrained γ-amino acid gababutin (Gbn) based peptides that form different conformations have been synthesized. Striving to rationalize the impact of side chain orientations framing tetrapeptide-based supramolecular organic frameworks and morphological entities, Gbn incorporated hybrid peptides Boc-Gbn-Aib-Aaa-Aib-OMe (where Aaa = Phe(F) for peptide 1, Leu(L) for peptide 2 and Tyr(Y) for peptide 3) were synthesized by changing the amino acid at the third position. The solution state dual folded conformation (C12 /C10 H-bonded) is probed by 2D NMR spectroscopy in support of a DMSO-d6 titration and VT NMR experiments...
February 19, 2018: Organic & Biomolecular Chemistry
Edjane R Dos Santos, Angelica E Graminha, Mario S Schultz, Isabel Correia, Heloisa S Selistre-de-Araújo, Rodrigo S Corrêa, Javier Ellena, Elisângela de Paula S Lacerda, João Costa Pessoa, Alzir A Batista
Thirteen new ruthenium amino acid complexes were synthesized and characterized. They were obtained by the reaction of α-amino acids (AA) with [RuCl2(P-P)(N-N)], where P-P=1,4-bis(diphenylphosphino)butane (dppb) or 1,3-bis(diphenylphosphino)propane (dppp) and N-N=4,4'-dimethyl-2,2'-bipyridine (4'-Mebipy), 5,5'-dimethyl-2,2'-bipyridine (5'-Mebipy) or 4,4'-Methoxy-2-2'-bipyridine (4'-MeObipy). This afforded a family of complexes formulated as [Ru(AA-H)(P-P)(N-N)]PF6, where AA=glycine (Gly), L-alanine (Ala), L-valine (Val), L-tyrosine (Tyr), L-tryptophan (Trp), L-histidine (His) and L-methionine (Met)...
December 23, 2017: Journal of Inorganic Biochemistry
Guillemette E Benoist, Eric van der Meulen, Inge M van Oort, Jan Hendrik Beumer, Diederik M Somford, Jack A Schalken, David M Burger, Nielka P van Erp
BACKGROUND: Enzalutamide is a potent androgen-signaling receptor inhibitor and is licensed for the treatment of metastatic castration-resistant prostate cancer. N-desmethylenzalutamide is the active metabolite of enzalutamide. A method to quantitate enzalutamide and its active metabolite was developed and validated according to the European Medicine Agency (EMA) guidelines. METHODS: Enzalutamide and N-desmethylenzalutamide were extracted by protein precipitation, separated on a C18 column with gradient elution and analyzed with tandem quadruple mass spectrometry in positive ion mode...
February 5, 2018: Therapeutic Drug Monitoring
Sang-Yoon Lee, Sunggyu Lee, Minkyu Choi, Kurunthachalam Kannan, Hyo-Bang Moon
Environmental contamination by siloxanes is a matter of concern due to their widespread consumption in personal care as well as industrial products and potential toxicity. Nevertheless, methods for simultaneous determination of cyclic and linear siloxanes in sediment are lacking. In this study, we developed an optimized analytical method to determine cyclic and linear siloxanes based on gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). This method was applied to determine concentrations of 19 siloxane compounds in surface and core sediments from industrialized bays in Korea to assess contamination status, spatial distribution, and historical trends...
January 27, 2018: Environmental Pollution
Celine Defaix, Audrey Solgadi, Thu Ha Pham, Alain M Gardier, Pierre Chaminade, Laurent Tritschler
In vivo measurement of multiple neurotransmitters is highly interesting but remains challenging in the field of neuroscience. GABA and l-glutamic acid are the major inhibitory and excitatory neurotransmitters, respectively, in the central nervous system, and their changes are related to a variety of diseases such as anxiety and major depressive disorder. This study described a simple method allowing the simultaneous LC-MS/MS quantification of l-glutamic acid, glutamine and GABA. Analytes were acquired from samples of the prefrontal cortex by microdialysis technique in freely moving mice...
January 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
Fumihiko Igarashi, Mari H Ogihara, Masatoshi Iga, Hiroshi Kataoka
Dietary sterols including cholesterol and phytosterols are essential substrates for insect steroid hormone (ecdysteroid) synthesis in the prothoracic glands (PGs). In a silkworm Bombyx mori, one of the model species of insects, the steroidogenesis has been well demonstrated that cholesterol biotransformation into ecdysone in the PG cells. Because insects lack the ability to synthesize cellular sterol de novo, lipoprotein, lipophorin (Lp), has been thought to be the major cholesterol supply source; however, details of cholesterol behavior from Lp to the PG cells has not been analyzed till date...
January 31, 2018: Steroids
Soo Jin Jeon, Fabio S Lima, Achilles Vieira-Neto, Vinicius S Machado, Svetlana F Lima, Rodrigo C Bicalho, Jose Eduardo P Santos, Klibs N Galvão
Broad-spectrum antibiotics such as ceftiofur and ampicillin are recommended for the treatment of metritis in dairy cows. Nonetheless, little is known about the impacts of antibiotics on the uterine microbiota. Here, we evaluated the shift in uterine microbiota after treating metritic cows with ceftiofur or ampicillin, and also gained insight into the uterine microbiota associated with cure of metritis. Uterine swabs from ceftiofur-treated, ampicillin-treated, and untreated metritic Holstein cows were collected on the day of metritis diagnosis (D1) and on D6 and then used for genomic DNA extraction and sequencing of the V4 hypervariable region of the 16S rRNA gene on the Illumina MiSeq platform...
February 2018: Veterinary Microbiology
Soumendrakrishna Karmahapatra, Corey Kientz, Shruthi Shetty, Jack C Yalowich, L Harinantenaina Rakotondraibe
Capsicodendrin (CPCD, 1), an epimeric mixture of a dimeric drimane-type sesquiterpene, is one of the major compounds present in the three endemic species of Madagascan traditional chemopreventive plants: Cinnamosma species (C. fragrans, C. macrocarpa, and C. madagascariensis). Despite the popular use of Cinnamosma in Madagascan traditional medicine and the reported antiproliferative properties of CPCD, elucidation of its mechanism(s) of action is still to be accomplished. In the present study, CPCD at low micromolar concentrations was cytotoxic and induced apoptosis in human myeloid leukemia cells in a time- and concentration-dependent manner...
February 6, 2018: Journal of Natural Products
Pritesh Contractor, Abhishek Gandhi, Gajendra Solanki, Priyanka A Shah, Pranav S Shrivastav
An accurate, sensitive and selective method is developed for determination of ergocalciferol (vitamin D2) in human plasma using LC-MS/MS. After liquid-liquid extraction with n-hexane, ergocalciferol was derivatized by reacting with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), a strong dienophile based on Diels-Alder reaction. Ergocalciferol and its deuterated internal standard, ergocalciferol-d6, were analyzed on X Select CSH C18 (100 mm×4.6 mm, 2.5 µm) column using acetonitrile and 0.1% (v/v) formic acid in water containing 0...
December 2017: Journal of Pharmaceutical Analysis
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