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Jane C Fenelon, Bruce D Murphy
Embryonic diapause is a common reproductive strategy amongst mammals, requiring an intimate cross-talk between the endometrium and the blastocyst. To date, the precise molecular signals responsible are unknown in the mouse or any other mammal. Previous studies in the mink implicate polyamines as major regulators of the control of diapause. In the mouse, inhibiting the rate-limiting enzyme of polyamine synthesis, ornithine decarboxylase (ODC1) during early pregnancy largely prevents implantation, but the fate of the non-implanted embryos is unknown...
June 16, 2017: Biology of Reproduction
Sara Díaz-Triviño, Yuchen Long, Ben Scheres, Ikram Blilou
In plant biology, transient expression systems have become valuable approaches used routinely to rapidly study protein expression, subcellular localization, protein-protein interactions, and transcriptional activity prior to in vivo studies. When studying transcriptional regulation, luciferase reporter assays offer a sensitive readout for assaying promoter behavior in response to different regulators or environmental contexts and to confirm and assess the functional relevance of predicted binding sites in target promoters...
2017: Methods in Molecular Biology
Valentina Salvi, Francesca Sozio, Silvano Sozzani, Annalisa Del Prete
Inflammatory reactions occurring in the central nervous system (CNS), known as neuroinflammation, are key components of the pathogenic mechanisms underlying several neurological diseases. The chemokine system plays a crucial role in the recruitment and activation of immune and non-immune cells in the brain, as well as in the regulation of microglia phenotype and function. Chemokines belong to a heterogeneous family of chemotactic agonists that signal through the interaction with G protein-coupled receptors (GPCRs)...
2017: Frontiers in Aging Neuroscience
Ahmed H Mageed, Brian W Skelton, Murray V Baker
Gold(iii) N-heterocyclic carbene (NHC) complexes of form [Au(NHC)4Cl2]Cl were synthesized by reaction of KAuCl4 with bis- and tetrakis(imidazolium) salts in the presence of a mild base. Treatment of these complexes with KPF6 afforded four-coordinate Au(III) complexes of form [Au(NHC)4](PF6)3. X-Ray crystallography showed the [Au(III)(NHC)4](3+) cations in the hexafluorophosphate salts to have a square planar Au(NHC)4 moiety [AuCNHC 2.024(4)-2.082(7) Å]. In the [Au(III)(NHC)4Cl2](+) cations in the chloride salts, coordination about Au was tetragonally-distorted octahedral, the axial Au-Cl bonds being substantially longer [AuCl 3...
June 8, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
Haoxu Dong, Zhiyan Zhong, Wei Chen, Xiao Wu, Qing Zhang, Guangying Huang, Wei Yang
This study was to explore the mechanism of acupuncture on regulating the endometrial angiogenesis and uterus dendritic cells (uDCs) during the peri-implantation period. Rats, in early pregnancy, were randomized into five groups: normal (N), model (M), acupuncture (A), progesterone (P), and A + P groups. The COH model was established using pregnant mare serum, combined with human chorionic gonadotrophin. Endometrium was collected on days 4, 6, and 8 (D4, D6, and D8) of gestation. Compared with group M, both VEGF and FGF-2 protein and mRNA levels were significantly lower on D4 and higher on D6 and D8 (P < 0...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
Chin-Pang Lee, Chia-Yih Liu, Ching-I Hung
BACKGROUND: It is unclear whether the 6-item Melancholia subscale of the Hamilton Depression Rating Scale (HAM-D6 ) has better psychometric properties than the parental 17-item version (HAM-D17 ) in the Chinese populations. METHODS: The study was to check the Chinese HAM-D17 and HAM-D6 for reliability and validity with Mokken scale analysis and item analysis. We reanalyzed a clinical sample of adult psychiatric outpatients (N = 214; age, mean (SD) = 38.3 (10...
June 6, 2017: Asia-Pacific Psychiatry: Official Journal of the Pacific Rim College of Psychiatrists
Xiaolin Chen, Chuangang Ning
It was believed that the rhenium anion Re(-) was not stable, just like Mn(-). We report the observation of Re(-) in the laser ablation ion source and the further confirmation via the high-resolution photoelectron spectra of Re(-). The ground state of Re(-) ions is 5d(6)6s(2 5)D4. The electron affinity of rhenium is determined to be 487.13(51) cm(-1) or 60.396(63) meV.
June 15, 2017: Journal of Physical Chemistry Letters
M C Max, C B Silva, S M González, A G Lindquist, I Búfalo, R G Gomes, F Morotti, C B Costa, Trr Barreiros, L A Lisboa, M M Seneda
This study investigated the effects of different concentrations of FSH (10, 50, 100 and 200 ng/ml) in supplemented MEM+ on the development of equine pre-antral follicles that were cultured in vitro for 2 or 6 days. The ovaries (n = 5) from mares in seasonal anoestrus were collected from a local abattoir. Ten ovarian tissue fragments of approximately 3 × 3 × 1 mm were obtained from each animal. The fragments were cultured in situ for 2 days (D2) or 6 days (D6) in MEM+ or MEM+ supplemented with FSH at four different concentrations, establishing the following 11 groups: control (D0); MEM + (D2); MEM + (D6); MEM + 10 ng/ml of FSH (D2); MEM + 10 ng/ml of FSH (D6); MEM + 50 ng/ml of FSH (D2); MEM + 50 ng/ml of FSH (D6); MEM + 100 ng/ml of FSH (D2); MEM + 100 ng/ml of FSH (D6); MEM + 200 ng/ml of FSH (D2); and MEM + 200 ng/ml of FSH (D6)...
June 4, 2017: Reproduction in Domestic Animals, Zuchthygiene
Jagrati Jain, Surendra K Jain, Larry A Walker, Babu L Tekwani
BACKGROUND: Protein ubiquitylation is an important post-translational regulation, which has been shown to be necessary for life cycle progression and survival of Plasmodium falciparum. Ubiquitin is a highly conserved 76 amino acid polypeptide, which attaches covalently to target proteins through combined action of three classes of enzymes namely, the ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin-protein ligase (E3). Ubiquitin E1 and E2 are highly conserved within eukaryotes...
June 2, 2017: BMC Pharmacology & Toxicology
Yaxi Hu, Shuo Wang, Shenlin Wang, Xiaonan Lu
Carcinogenic Sudan I has been added illegally into spices for an apparent freshness. (1)H solution and solid-state (SS) nuclear magnetic resonance (NMR) spectroscopies were applied and compared for determination of Sudan I in paprika powders (PPs). For solution NMR, PPs spiked with Sudan I were extracted with acetonitrile, centrifuged, rotor-evaporated, and re-dissolved in DMSO-d6 for spectral collection. For SSNMR, Sudan I contaminated PPs were mixed with DMSO-d6 solution and used for spectral collection. Linear regression models constructed for quantitative analyses resulted in the average accuracies for unknown samples as 98% and 105%, respectively...
June 1, 2017: Scientific Reports
David A Marsh, William S Elliott, Rachel M Smith, Meredith C Sharps, Mary K Baumeister, Matthew E Carnes, Lev N Zakharov, William H Casey, Darren William Johnson
Large aqueous inorganic cluster ions are interesting due to their utility in materials science (for example to generate thin films), and also because they serve as molecular models for the oxide-aqueous mineral interface where spectroscopy is inherently difficult. Here we show that new clusters of the type M[(μ-OH)2Co(NH3)4]3(NO3)6 (M = Al, Ga) can be synthesized using Werner's century-old cluster as a substitutable framework. We substituted Group 13 metals into the Hexol ion, [Co((μ-OH)2Co(NH3)4)3]6+, to make diamagnetic heterometallic ions...
June 1, 2017: Angewandte Chemie
Takashi Ishizu, Hiroyuki Tsutsumi, Emi Yokoyama, Haruka Kawamoto, Runa Yokota
In water, diketopiperazines cyclo(L-Pro-L-Xxx) and cyclo(L-Pro-D-Xxx) (Xxx=Phe, Tyr) formed an intramolecular hydrophobic interaction between the main skeleton part and their benzene ring, and both cyclo(L-Pro-L-Xxx) and cyclo(L-Pro-D-Xxx) took a folded conformation. The conformational changes from folded to extended conformation by addition of several deuterated organic solvents (acetone-d6, metanol-d4, dimethyl sulfoxide-d6 (DMSO-d6)) and the temperature rise were investigated using (1)H-NMR spectra. The results suggested that the intrarmolecular hydrophobic interaction of cyclo(L-Pro-D-Xxx) formed more strongtly than that of cyclo(L-Pro-L-Xxx)...
2017: Chemical & Pharmaceutical Bulletin
Ying Liu, Andrea Lucas-Hahn, Bjoern Petersen, Rong Li, Doris Hermann, Petra Hassel, Maren Ziegler, Knud Larsen, Heiner Niemann, Henrik Callesen
The "Dolly" based cloning (classical nuclear transfer, [CNT]) and the handmade cloning (HMC) are methods that are nowadays routinely used for somatic cloning of large domestic species. Both cloning protocols share several similarities, but differ with regard to the required in vitro culture, which in turn results in different time intervals until embryo transfer. It is not yet known whether the differences between cloned embryos from the two protocols are due to the cloning methods themselves or the in vitro culture, as some studies have shown detrimental effects of in vitro culture on conventionally produced embryos...
June 2017: Cellular Reprogramming
Eun-Kyung Choi, Jae-Hyung Lee, Seung-Hak Baek, Su-Jung Kim
INTRODUCTION: We explored the gene expression profile altered by orthodontic tooth movement (OTM) during the healing of surgical alveolar defects in beagles. METHODS: An OTM-related healing model was established where a maxillary second premolar was protracted into the critical-sized defect for 6 weeks (group DT6). As controls, natural healing models without OTM were set at 2 weeks (group D2) and at 6 weeks (group D6) after surgery. Total RNAs were extracted from dissected tissue blocks containing the regenerated defects and additionally from sound alveolar bone as a baseline (group C)...
June 2017: American Journal of Orthodontics and Dentofacial Orthopedics
Xuemeng Xie, Jinfu Tu, Heyi You, Bingren Hu
Given the important role that inhibitory kappa B (IκB) kinase β (IKKβ) plays in pancreatic cancer (PC) development and progression, inhibitors targeting IKKβ are believed to be increasingly popular as novel anti-PC therapies. Two synthetic molecules, named EF24 and EF31, exhibited favorable potential in terms of inhibition of both IKKβ activity and PC cell proliferation. Aiming to enhance their cellular efficacy and to analyze their structure-activity relationship, four series of EF24 and EF31 analogs were designed and synthesized...
2017: Drug Design, Development and Therapy
Agnieszka Cholewiak, Agnieszka Tycz, Janusz Jurczak
The DITIPIRAM (8-propyldithieno[3,2-b:2',3'-e]pyridine-3,5-diamine)-based receptors 11 and 12 were readily synthesized, and their anion-binding properties were studied both in solution and in the solid-state. (1)H NMR titrations revealed that receptor 12 equipped with two phenyl-urea groups preferentially binds carboxylates, even in the highly competitive DMSO-d6/CD3OH solvent mixture. X-ray analysis showed that receptor 12 exhibited great complementarity for benzoate, which is cooperatively bound by the means of four highly directional hydrogen bonds from the two urea groups...
May 24, 2017: Organic Letters
Birgit Prodinger, Alan Tennant, Gerold Stucki
BACKGROUND: In clinical practice and research a variety of clinical data collection tools are used to collect information on people's functioning for clinical practise and research and National Health Information Systems. Reporting on ICF-based common metrics enables standardized documentation of functioning information in national health information systems. OBJECTIVE: The objective of this methodological note on applying the ICF in rehabilitation is to demonstrate how to report functioning information collected with a data collection tool on ICF-based common metrics...
May 23, 2017: European Journal of Physical and Rehabilitation Medicine
Caitlyn Makins, Douglas A Whitelaw, Michael McGregor, Alix Petit, Robert G Mothersole, Kathleen E Prosser, Kirsten R Wolthers
Ornithine 4,5-aminomutase (OAM) from Clostridium sticklandii is an adenosylcobalamin (AdoCbl) and pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes a 1,2-amino shift, interconverting d-ornithine and 2S, 4R-diaminopentanoate. The reaction occurs via a radical-based mechanism whereby a PLP-bound substrate radical undergoes intramolecular isomerization via an azacyclopropylcarbinyl radical intermediate. Herein, we investigated the catalytic role of active site residues that form non-covalent interactions with PLP and/or substrate, d-ornithine...
August 2017: Biochimica et Biophysica Acta
Erhan Tenekecioglu, Patrick W Serruys, Yoshinobu Onuma, Ricardo Costa, Daniel Chamié, Yohei Sotomi, Ting-Bin Yu, Alexander Abizaid, Houng-Bang Liew, Teguh Santoso
OBJECTIVES: The primary objective of this study was to evaluate the safety and effectiveness of the Mirage bioresorbable microfiber sirolimus-eluting scaffold compared with the Absorb bioresorbable vascular scaffold in the treatment of stenotic target lesions located in native coronary arteries, ranging from ≥2.25 to ≤4.0 mm in diameter. Secondary objectives were to establish the medium-term safety, effectiveness, and performance of the Mirage device. BACKGROUND: The current generation of bioresorbable scaffolds has several limitations, such as thick square struts with large footprints that preclude their deep embedment into the vessel wall, resulting in protrusion into the lumen with microdisturbance of flow...
May 11, 2017: JACC. Cardiovascular Interventions
Sana Shaikh, Naseem Aslam Channa, Farha Naz Talpur, Muhammad Younis, Naila Tabassum
BACKGROUND: Breast cancer is a disease with diverse clinical symptoms, molecular profiles, and its nature to response its therapeutic treatments. Radiotherapy (RT), along with surgery and chemotherapy is a part of treatment in breast cancer. The aim of present study was to investigate pre and post treatment effects of radiotherapy in serum fatty acids and its lipids profile in patients with breast cancer. METHODS: In this comparative as well as follow up study, Serum fatty acids were performed by gas chromatography to investigate fatty acids and Microlab for analysis of lipid profile...
May 18, 2017: Lipids in Health and Disease
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