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osteoarthritis stem cells

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https://www.readbyqxmd.com/read/28319248/cyclic-tensile-strain-enhances-human-mesenchymal-stem-cell-smad-2-3-activation-and-tenogenic-differentiation-in-anisotropic-collagen-glycosaminoglycan-scaffolds
#1
W G Grier, A S Moy, B A Harley
Stem cell research arose from the need to explore new therapeutic possibilities for intractable and lethal diseases. Although musculoskeletal disorders are basically nonlethal, their high prevalence and relative ease of performing clinical trials have facilitated the clinical application of stem cells in this field. However, few reliable clinical studies have been published, despite the plethora of in vitro and preclinical studies in stem cell research for regenerative medicine in the musculoskeletal system...
March 20, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28297568/safety-studies-for-use-of-adipose-tissue-derived-mesenchymal-stromal-stem-cells-in-a-rabbit-model-for-osteoarthritis-to-support-a-phase-i-clinical-trial
#2
Scott M Riester, Janet M Denbeigh, Yang Lin, Dakota L Jones, Tristan de Mooij, Eric A Lewallen, Hai Nie, Christopher R Paradise, Darcie J Radel, Amel Dudakovic, Emily T Camilleri, Dirk R Larson, Wenchun Qu, Aaron J Krych, Matthew A Frick, Hee-Jeong Im, Allan B Dietz, Jay Smith, Andre J van Wijnen
Adipose-derived mesenchymal stem cells (AMSCs) offer potential as a therapeutic option for clinical applications in musculoskeletal regenerative medicine because of their immunomodulatory functions and capacity for trilineage differentiation. In preparation for a phase I clinical trial using AMSCs to treat patients with osteoarthritis, we carried out preclinical studies to assess the safety of human AMSCs within the intra-articular joint space. Culture-expanded human AMSCs grown in human platelet-lysate were delivered via intra-articular injections into normal healthy rabbit knees and knees at risk for the development of osteoarthritis after bilateral medial anterior hemimeniscectomy...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28279188/comparison-of-exosomes-secreted-by-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells-and-synovial-membrane-derived-mesenchymal-stem-cells-for-the-treatment-of-osteoarthritis
#3
Yu Zhu, Yuchen Wang, Bizeng Zhao, Xin Niu, Bin Hu, Qing Li, Juntao Zhang, Jian Ding, Yunfeng Chen, Yang Wang
BACKGROUND: Osteoarthritis (OA) is the most common joint disease worldwide. In the past decade, mesenchymal stem cells (MSCs) have been used widely for the treatment of OA. A potential mechanism of MSC-based therapies has been attributed to the paracrine secretion of trophic factors, in which exosomes may play a major role. In this study, we aimed to compare the effectiveness of exosomes secreted by synovial membrane MSCs (SMMSC-Exos) and exosomes secreted by induced pluripotent stem cell-derived MSCs (iMSC-Exos) on the treatment of OA...
March 9, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28278383/3d-printed-poly-%C3%AE%C2%B5-caprolactone-scaffold-augmented-with-mesenchymal-stem-cells-for-total-meniscal-substitution
#4
Zheng-Zheng Zhang, Shao-Jie Wang, Ji-Ying Zhang, Wen-Bo Jiang, Ai-Bing Huang, Yan-Song Qi, Jian-Xun Ding, Xue-Si Chen, Dong Jiang, Jia-Kuo Yu
BACKGROUND: Total meniscectomy leads to knee osteoarthritis in the long term. The poly(ε-caprolactone) (PCL) scaffold is a promising material for meniscal tissue regeneration, but cell-free scaffolds result in relatively poor tissue regeneration and lead to joint degeneration. HYPOTHESIS: A novel, 3-dimensional (3D)-printed PCL scaffold augmented with mesenchymal stem cells (MSCs) would offer benefits in meniscal regeneration and cartilage protection. STUDY DESIGN: Controlled laboratory study...
March 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28266690/lengthening-primary-cilia-enhances-cellular-mechanosensitivity
#5
G-I Im
Stem cell research arose from the need to explore new therapeutic possibilities for intractable and lethal diseases. Although musculoskeletal disorders are basically nonlethal, their high prevalence and relative ease of performing clinical trials have facilitated the clinical application of stem cells in this field. However, few reliable clinical studies have been published, despite the plethora of in vitro and preclinical studies in stem cell research for regenerative medicine in the musculoskeletal system...
February 21, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28258665/ppar-%C3%AE-agonist-with-mesenchymal-stem-cells-induces-type-ii-collagen-producing-chondrocytes-in-human-arthritic-synovial-fluid
#6
Bruce E Heck, Joshua J Park, Vishruti Makani, Eun-Cheol Kim, Dong Hyun Kim
Osteoarthritis (OA) is an inflammatory joint disease characterized by degeneration of articular cartilage within synovial joints. An estimated 27 million Americans suffer from OA and the population is expected to reach 67 million in USA by 2030. Thus, it is urgent to find an effective OA treatment. Traditional OA treatments have no disease-modifying effect while regenerative OA therapies such as autologous chondrocyte implantation (ACI) show some promise. Nonetheless, current regenerative therapies do not overcome synovial inflammation that suppresses the differentiation of mesenchymal stem cells (MSCs) to chondrocytes and the expression of type II collagen, the major constituent of functional cartilage...
March 3, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28258177/stem-cell-injections-in-knee-osteoarthritis-a-systematic-review-of-the-literature
#7
REVIEW
Haiko Imfl Pas, Marinus Winters, Hidde J Haisma, Martinus Jj Koenis, Johannes L Tol, Maarten H Moen
OBJECTIVE: Stem cell injection for knee osteoarthritis (KOA) is an emerging new therapy, and we aimed to review its evidence of efficacy. DESIGN: Systematic review. ELIGIBILITY CRITERIA: Criteria for eligibility were randomised controlled trials (RCTs) and non-RCT on the efficacy of stem cell injections in KOA. All references were checked for missed articles. DATA SOURCES: MEDLINE, EMBASE, CINAHL, Web of Science, Cochrane Library, PEDro and SPORTDiscus were searched...
March 3, 2017: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/28256634/monomeric-porous-type-ii-collagen-scaffolds-promote-chondrogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-in-vitro
#8
M Tamaddon, M Burrows, S A Ferreira, F Dazzi, J F Apperley, A Bradshaw, D D Brand, J Czernuszka, E Gentleman
Osteoarthritis (OA) is a common cause of pain and disability and is often associated with the degeneration of articular cartilage. Lesions to the articular surface, which are thought to progress to OA, have the potential to be repaired using tissue engineering strategies; however, it remains challenging to instruct cell differentiation within a scaffold to produce tissue with appropriate structural, chemical and mechanical properties. We aimed to address this by driving progenitor cells to adopt a chondrogenic phenotype through the tailoring of scaffold composition and physical properties...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28243954/knee-cartilage-regeneration-with-umbilical-cord-mesenchymal-stem-cells-embedded-in-collagen-scaffold-using-dry-arthroscopy-technique
#9
B Sadlik, G Jaroslawski, D Gladysz, M Puszkarz, M Markowska, K Pawelec, D Boruczkowski, T Oldak
Articular cartilage injuries lead to progressive degeneration of the joint with subsequent progression to osteoarthritis, which currently becomes a serious health and economic issue. Due to limited capability for self-regeneration, cartilage repair remains a challenge for the present-day orthopedics. Currently, available therapeutic methods fail to provide satisfactory results. A search for other strategies that could regenerate a hyaline-like tissue with a durable effect and adequate mechanical properties is underway...
February 28, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28243085/continuous-release-of-bone-morphogenetic-protein-2-through-nano-graphene-oxide-based-delivery-influences-the-activation-of-the-nf-%C3%AE%C2%BAb-signal-transduction-pathway
#10
Cheng Zhong, Jun Feng, Xiangjin Lin, Qi Bao
Graphene oxide (GO) has been used as a delivery vehicle for small molecule drugs and nucleotides. To further investigate GO as a smart biomaterial for the controlled release of cargo molecules, we hypothesized that GO may be an appropriate delivery vehicle because it releases bone morphogenetic protein 2 (BMP2). GO characterization indicated that the size distribution of the GO flakes ranged from 81.1 nm to 45,749.7 nm, with an approximate thickness of 2 nm. After BMP2 adsorption onto GO, Fourier-transformed infrared spectroscopy (FTIR) and thermal gravimetric analysis were performed...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28241885/repeated-intra-articular-injection-of-allogeneic-mesenchymal-stem-cells-causes-an-adverse-response-compared-to-autologous-cells-in-the-equine-model
#11
Amanda-Jo Joswig, Alexis Mitchell, Kevin J Cummings, Gwendolyn J Levine, Carl A Gregory, Roger Smith, Ashlee E Watts
BACKGROUND: Intra-articular injection of mesenchymal stem cells (MSCs) is efficacious in osteoarthritis therapy. A direct comparison of the response of the synovial joint to intra-articular injection of autologous versus allogeneic MSCs has not been performed. The objective of this study was to assess the clinical response to repeated intra-articular injection of allogeneic versus autologous MSCs prepared in a way to minimize xeno-contaminants in a large animal model. METHODS: Intra-articular injections of bone marrow-derived, culture-expanded MSCs to a forelimb metacarpophalangeal joint were performed at week 0 and week 4 (six autologous; six autologous with xeno-contamination; six allogeneic)...
February 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28225870/pdk2-promotes-chondrogenic-differentiation-of-mesenchymal-stem-cells-by-upregulation-of-sox6-and-activation-of-jnk-mapk-erk-pathway
#12
H Wang, X B Shan, Y J Qiao
This study was undertaken to clarify the role and mechanism of pyruvate dehydrogenase kinase isoform 2 (PDK2) in chondrogenic differentiation of mesenchymal stem cells (MSCs). MSCs were isolated from femurs and tibias of Sprague-Dawley rats, weighing 300-400 g (5 females and 5 males). Overexpression and knockdown of PDK2 were transfected into MSCs and then cell viability, adhesion and migration were assessed. Additionally, the roles of aberrant PDK2 in chondrogenesis markers SRY-related high mobility group-box 6 (Sox6), type ΙΙ procollagen gene (COL2A1), cartilage oligomeric matrix protein (COMP), aggrecan (AGC1), type ΙX procollagen gene (COL9A2) and collagen type 1 alpha 1 (COL1A1) were measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR)...
February 16, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28207735/long-non-coding-rna-hotair-inhibits-mir-17-5p-to-regulate-osteogenic-differentiation-and-proliferation-in-non-traumatic-osteonecrosis-of-femoral-head
#13
Biaofang Wei, Wei Wei, Baoxiang Zhao, Xiaxia Guo, Song Liu
BACKGROUND AND AIM: The biological functions of non-coding RNAs (ncRNAs) have been widely identified in many human diseases. In the present study, the relationship between long non-coding RNA HOTAIR and microRNA-17-5p (miR-17-5p) and their roles in osteogenic differentiation and proliferation in non-traumatic osteonecrosis of femoral head (ONFH) were investigated. METHODS: The expression levels of HOTAIR and miR-17-5p in the mesenchymal stem cells (MSCs) derived from patients with non-traumatic ONFH and osteoarthritis (OA) were examined by real-time PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28203597/intra-articular-implantation-of-mesenchymal-stem-cells-part-1-a-review-of-the-literature-for-prevention-of-postmeniscectomy-osteoarthritis
#14
Matthew J Kraeutler, Justin J Mitchell, Jorge Chahla, Eric C McCarty, Cecilia Pascual-Garrido
Osteoarthritis (OA) after a partial or total meniscectomy procedure is a common pathology. Because of the high incidence of meniscectomy in the general population, as well as the significant burden of knee OA, there is increasing interest in determining methods for delaying postmeniscectomy OA. Biological therapies, including mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs), and platelet-rich plasma (PRP), have been proposed as possible therapies that could delay OA in this and other settings...
January 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28203596/intra-articular-implantation-of-mesenchymal-stem-cells-part-2-a-review-of-the-literature-for-meniscal-regeneration
#15
Matthew J Kraeutler, Justin J Mitchell, Jorge Chahla, Eric C McCarty, Cecilia Pascual-Garrido
Knee osteoarthritis (OA) after partial or total meniscectomy is a prevalent issue that patients must face. Various methods of replacing meniscal tissue have been studied to avoid this progression, including meniscal allograft transplantation, meniscal scaffolds, and synthetic meniscus replacement. Studies have shown that meniscal scaffolds may improve symptoms but have not been shown to prevent progression of OA. Recently, mesenchymal stem cells (MSCs) have been proposed as a possible biological therapy for meniscal regeneration...
January 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28197017/characterization-and-therapeutic-application-of-canine-adipose-mesenchymal-stem-cells-to-treat-elbow-osteoarthritis
#16
Éva Kriston-Pál, Ágnes Czibula, Zoltán Gyuris, Gyula Balka, Antal Seregi, Farkas Sükösd, Miklós Süth, Endre Kiss-Tóth, Lajos Haracska, Ferenc Uher, Éva Monostori
Visceral adipose tissue (AT) obtained from surgical waste during routine ovariectomies was used as a source for isolating canine mesenchymal stem cells (MSCs). As determined by cytofluorimetry, passage 2 cells expressed MSC markers CD44 and CD90 and were negative for lineage-specific markers CD34 and CD45. The cells differentiated toward osteogenic, adipogenic, and chondrogenic directions. With therapeutic aims, 30 dogs (39 joints) suffering from elbow dysplasia (ED) and osteoarthritis (OA) were intra-articularly transplanted with allogeneic MSCs suspended in 0...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28193542/sulfated-hyaluronic-acid-hydrogels-with-retarded-degradation-and-enhanced-growth-factor-retention-promote-hmsc-chondrogenesis-and-articular-cartilage-integrity-with-reduced-hypertrophy
#17
Qian Feng, Sien Lin, Kunyu Zhang, Chaoqun Dong, Tianyi Wu, Heqin Huang, Xiaohui Yan, Li Zhang, Gang Li, Liming Bian
Recently, hyaluronic acid (HA) hydrogels have been extensively researched for delivering cells and drugs to repair damaged tissues, particularly articular cartilage. However, the in vivo degradation of HA is fast, thus limiting the clinical translation of HA hydrogels. Furthermore, HA cannot bind proteins with high affinity because of the lack of negatively charged sulfate groups. In this study, we conjugated sulfate groups to HA. These sulfated HA exhibit significantly slower degradation by hyaluronidase compared to the wild type HA...
February 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28191757/cartilage-regeneration-in-osteoarthritic-patients-by-a-composite-of-allogeneic-umbilical-cord-blood-derived-mesenchymal-stem-cells-and-hyaluronate-hydrogel-results-from-a-clinical-trial-for-safety-and-proof-of-concept-with-7-years-of-extended-follow-up
#18
Yong-Beom Park, Chul-Won Ha, Choong-Hee Lee, Young Cheol Yoon, Yong-Geun Park
Few methods are available to regenerate articular cartilage defects in patients with osteoarthritis. We aimed to assess the safety and efficacy of articular cartilage regeneration by a novel medicinal product composed of allogeneic human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs). Patients with Kellgren-Lawrence grade 3 osteoarthritis and International Cartilage Repair Society (ICRS) grade 4 cartilage defects were enrolled in this clinical trial. The stem cell-based medicinal product (a composite of culture-expanded allogeneic hUCB-MSCs and hyaluronic acid hydrogel [Cartistem]) was applied to the lesion site...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28188861/functions-of-rho-family-of-small-gtpases-and-rho-associated-coiled-coil-kinases-in-bone-cells-during-differentiation-and-mineralization
#19
REVIEW
Agnieszka Strzelecka-Kiliszek, Saida Mebarek, Monika Roszkowska, René Buchet, David Magne, Slawomir Pikula
BACKGROUND: Members of Rho-associated coiled-coil kinases (ROCKs) are effectors of Rho family of small GTPases. ROCKs have multiple functions that include regulation of cellular contraction and polarity, adhesion, motility, proliferation, apoptosis, differentiation, maturation and remodeling of the extracellular matrix (ECM). SCOPE OF THE REVIEW: Here, we focus on the action of RhoA and RhoA effectors, ROCK1 and ROCK2, in cells related to tissue mineralization: mesenchymal stem cells, chondrocytes, preosteoblasts, osteoblasts, osteocytes, lining cells and osteoclasts...
February 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28186682/repair-of-torn-avascular-meniscal-cartilage-using-undifferentiated-autologous-mesenchymal-stem-cells-from-in-vitro-optimization-to-a-first-in-human-study
#20
Michael R Whitehouse, Nicholas R Howells, Michael C Parry, Eric Austin, Wael Kafienah, Kyla Brady, Allen E Goodship, Jonathan D Eldridge, Ashley W Blom, Anthony P Hollander
Meniscal cartilage tears are common and predispose to osteoarthritis (OA). Most occur in the avascular portion of the meniscus where current repair techniques usually fail. We described previously the use of undifferentiated autologous mesenchymal stem cells (MSCs) seeded onto a collagen scaffold (MSC/collagen-scaffold) to integrate meniscal tissues in vitro. Our objective was to translate this method into a cell therapy for patients with torn meniscus, with the long-term goal of delaying or preventing the onset of OA...
December 15, 2016: Stem Cells Translational Medicine
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