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osteoarthritis stem cells

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https://www.readbyqxmd.com/read/29774080/microrna-193b-3p-regulates-chondrogenesis-and-chondrocyte-metabolism-by-targeting-hdac3
#1
Fangang Meng, Zhiwen Li, Zhiqi Zhang, Zibo Yang, Yan Kang, Xiaoyi Zhao, Dianbo Long, Shu Hu, Minghui Gu, Suiwen He, Peihui Wu, Zongkun Chang, Aishan He, Weiming Liao
Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression in human chondrocytes. The aim of this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates the expression of HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p expression was assessed in a human mesenchymal stem cell (hMSC) model of chondrogenesis, in interleukin-1β (IL-1β)-treated primary human chondrocytes (PHCs), and in non-degraded and degraded cartilage...
2018: Theranostics
https://www.readbyqxmd.com/read/29765416/combating-osteoarthritis-through-stem-cell-therapies-by-rejuvenating-cartilage-a-review
#2
REVIEW
Navneet Kumar Dubey, Viraj Krishna Mishra, Rajni Dubey, Shabbir Syed-Abdul, Joseph R Wang, Peter D Wang, Win-Ping Deng
Knee osteoarthritis (OA) is a chronic degenerative disorder which could be distinguished by erosion of articular cartilage, pain, stiffness, and crepitus. Not only aging-associated alterations but also the metabolic factors such as hyperglycemia, dyslipidemia, and obesity affect articular tissues and may initiate or exacerbate the OA. The poor self-healing ability of articular cartilage due to limited regeneration in chondrocytes further adversely affects the osteoarthritic microenvironment. Traditional and current surgical treatment procedures for OA are limited and incapable to reverse the damage of articular cartilage...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29763932/microvesicles-from-human-adipose-tissue-derived-mesenchymal-stem-cells-as-a-new-protective-strategy-in-osteoarthritic-chondrocytes
#3
Miguel Tofiño-Vian, Maria Isabel Guillén, María Dolores Pérez Del Caz, Antonio Silvestre, María José Alcaraz
BACKGROUND/AIMS: Chronic inflammation contributes to cartilage degeneration during the progression of osteoarthritis (OA). Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. METHODS: AD-MSC were isolated by collagenase treatment from adipose tissue from healthy individuals subjected to abdominal lipectomy surgery...
May 9, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29761573/using-fat-to-fight-disease-a-systematic-review-of-non-homologous-adipose-derived-stromal-stem-cell-therapies
#4
REVIEW
Marjorie E Bateman, Amy L Strong, Jeffrey M Gimble, Bruce A Bunnell
OBJECTIVE: The objective of this review is to describe the safety and efficacy of ASC and SVF in treating common diseases and the next steps in research that must occur prior to clinical use. EVIDENCE REVIEW: Pubmed, Ovid Medline, Embase, Web of Science, and the Cochrane Library were searched for articles about use of SVF or ASC for disease therapy published between 2012 and 2017. One meta-analysis, 2 randomized controlled trials, and 16 case series were included, representing 844 human patients...
May 15, 2018: Stem Cells
https://www.readbyqxmd.com/read/29760725/the-effects-of-hyperacute-serum-on-the-elements-of-the-human-subchondral-bone-marrow-niche
#5
Melinda Simon, Bálint Major, Gabriella Vácz, Olga Kuten, István Hornyák, Adél Hinsenkamp, Dorottya Kardos, Marcell Bagó, Domonkos Cseh, Adrienn Sárközi, Denes Horvathy, Stefan Nehrer, Zsombor Lacza
Mesenchymal stem cells (MSCs) are widely used in laboratory experiments as well as in human cell therapy. Their culture requires animal sera like fetal calf serum (FCS) as essential supplementation; however, animal sera pose a risk for clinical applications. Human blood derivatives, for example, platelet-rich plasma (PRP) releasates, are potential replacements of FCS; however, it is unclear which serum variant has the best effect on the given cell or tissue type. Additionally, blood derivatives are commonly used in musculoskeletal diseases like osteoarthritis (OA) or osteonecrosis as "proliferative agents" for the topical MSC pool...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29754173/treating-age-related-diseases-with-somatic-stem-cells
#6
Robert W Brooks, Paul D Robbins
Life expectancy in the developed world has advanced beyond the number of years in which healthy tissue homeostasis can be maintained, and as a result, the number of persons with severe and debilitating chronic illnesses, including cancer, diabetes, osteoarthritis, osteoporosis, neurodegenerative and cardiovascular disease has continued to rise. One of the key underlying causes for the loss in the ability to replenish damaged tissues is the qualitative and quantitative decline in somatic stem cell populations...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29748647/isolation-and-functional-assessment-of-mouse-skeletal-stem-cell-lineage
#7
Gunsagar S Gulati, Matthew P Murphy, Owen Marecic, Michael Lopez, Rachel E Brewer, Lauren S Koepke, Anoop Manjunath, Ryan C Ransom, Ankit Salhotra, Irving L Weissman, Michael T Longaker, Charles K F Chan
There are limited methods available to study skeletal stem, progenitor, and progeny cell activity in normal and diseased contexts. Most protocols for skeletal stem cell isolation are based on the extent to which cells adhere to plastic or whether they express a limited repertoire of surface markers. Here, we describe a flow cytometry-based approach that does not require in vitro selection and that uses eight surface markers to distinguish and isolate mouse skeletal stem cells (mSSCs); bone, cartilage, and stromal progenitors (mBCSPs); and five downstream differentiated subtypes, including chondroprogenitors, two types of osteoprogenitors, and two types of hematopoiesis-supportive stroma...
June 2018: Nature Protocols
https://www.readbyqxmd.com/read/29747006/bioinspired-bone-therapies-using-naringin-applications-and-advances
#8
REVIEW
Pedro Lavrador, Vitor M Gaspar, João F Mano
The use of natural compounds for treating chronic bone diseases holds remarkable potential. Among these therapeutics, naringin, a flavanone glycoside, represents one of the most promising candidates owing to its multifaceted effect on bone tissues. This review provides an up-to-date overview on naringin applications in the treatment of bone disorders, such as osteoporosis and osteoarthritis, and further highlights its potential for stem cell pro-osteogenic differentiation therapies. A critical perspective on naringin clinical translation is also provided...
May 7, 2018: Drug Discovery Today
https://www.readbyqxmd.com/read/29736567/advances-for-treatment-of-knee-oc-defects
#9
Marta Ondrésik, J Miguel Oliveira, Rui L Reis
Osteochondral (OC) defects are prevalent among young adults and are notorious for being unable to heal. Although they are traumatic in nature, they often develop silently. Detection of many OC defects is challenging, despite the criticality of early care. Current repair approaches face limitations and cannot provide regenerative or long-standing solution. Clinicians and researchers are working together in order to develop approaches that can regenerate the damaged tissues and protect the joint from developing osteoarthritis...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29736207/interleukin-6-from-subchondral-bone-mesenchymal-stem-cells-contributes-to-the-pathological-phenotypes-of-experimental-osteoarthritis
#10
Xiaofeng Wu, Lei Cao, Fan Li, Chao Ma, Guangwang Liu, Qiugen Wang
As a main cause of morbidity in the aged population, osteoarthritis (OA) is characterized by cartilage destruction, synovium inflammation, osteophytes, and subchondral bone sclerosis. To date its etiology remains elusive. Recent data highlight an important role of subchondral bone in the onset and progression of OA. Therefore, elucidating the mechanisms underlying abnormal subchondral bone could be of importance in the treatment of OA. Interleukin-6 is a proinflammatory cytokine involved in many physiological and pathological processes...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29728632/molecular-characterization-of-mesenchymal-stem-cells-in-human-osteoarthritis-cartilage-reveals-contribution-to-the-oa-phenotype
#11
Chathuraka T Jayasuriya, Nan Hu, Jing Li, Nicholas Lemme, Richard Terek, Michael G Ehrlich, Qian Chen
Adult human articular cartilage harbors a population of CD166+ mesenchymal stem cell-like progenitors that become more numerous during osteoarthritis (OA). While their role is not well understood, here we report that they are indeed part of cellular clusters formed in OA cartilage, which is a pathological hallmark of this disease. We hypothesize that these cells, termed OA mesenchymal stem cells (OA-MSCs), contribute to OA pathogenesis. To test this hypothesis, we generated and characterized multiple clonally derived stable/immortalized human OA-MSC cell lines, which exhibited the following properties...
May 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29725971/a-comprehensive-review-of-stem-cells-for-cartilage-regeneration-in-osteoarthritis
#12
Gauthaman Kalamegam, Adnan Memic, Emma Budd, Mohammed Abbas, Ali Mobasheri
Osteoarthritis (OA) is an age related joint disease associated with degeneration and loss of articular cartilage. Consequently, OA patients suffer from chronic joint pain and disability. Weight bearing joints and joints that undergo repetitive stress and excessive 'wear and tear' are particularly prone to developing OA. Cartilage has a poor regenerative capacity and current pharmacological agents only provide symptomatic pain relief. OA patients that respond poorly to conventional therapies are ultimately treated with surgical procedures to promote cartilage repair by implantation of artificial joint structures (arthroplasty) or total joint replacement (TJR)...
May 4, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29725360/-ajuga-decumbens-stimulates-mesenchymal-stem-cell-differentiation-and-regenerates-cartilage-in-a-rabbit-osteoarthritis-model
#13
Yoko Sawada, Atsushi Sugimoto, Tomohiro Osaki, Yoshiharu Okamoto
In a previous study by our group, Ajuga decumbens extract (ADE) was demonstrated to decrease the number of osteoclasts in subchondral bone and to have a synergistic effect with glucosamine in improving cartilaginous injuries in a rabbit model of osteoarthritis. In the present study, a concentrate of the useful fraction of ADE, termed extra ADE (EADE), which includes higher concentrations of the active component 20-hydroxyecdysone, was evaluated for its efficacy to accelerate the healing of experimental cartilage injury...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29720268/specific-markers-and-properties-of-synovial-mesenchymal-stem-cells-in-the-surface-stromal-and-perivascular-regions
#14
Mitsuru Mizuno, Hisako Katano, Yo Mabuchi, Yusuke Ogata, Shizuko Ichinose, Shizuka Fujii, Koji Otabe, Keiichiro Komori, Nobutake Ozeki, Hideyuki Koga, Kunikazu Tsuji, Chihiro Akazawa, Takeshi Muneta, Ichiro Sekiya
BACKGROUND: Synovial mesenchymal stem cells (MSCs) are an attractive cell source for cartilage and meniscus regeneration. Synovial tissue can be histologically classified into three regions; surface, stromal and perivascular region, but the localization of synovial MSCs has not been fully investigated. We identified markers specific for each region, and compared properties of MSCs derived from each region in the synovium. METHODS: The intensity of immunostaining with 19 antibodies was examined for surface, stromal, and perivascular regions of human synovium from six osteoarthritis patients...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29713784/limited-evidence-for-adipose-derived-stem-cell-therapy-on-the-treatment-of-osteoarthritis
#15
REVIEW
Eoghan T Hurley, Youichi Yasui, Arianna L Gianakos, Dexter Seow, Yoshiharu Shimozono, Gino M M J Kerkhoffs, John G Kennedy
PURPOSE: The purpose of this systematic review is to evaluate the effects of adipose derived mesenchymal stem cells (ADSCs) in the treatment of osteoarthritis (OA) in the clinical setting. METHODS: A literature search was performed in the MEDLINE, EMBASE, and The Cochrane Library Database up to January 2017 for inclusion and exclusion criteria. Criteria for inclusion were clinical studies demonstrating the effects of ADSCs on OA, and written in English. The following variables were analyzed: donor site, volume of adipose tissue, preparation of ADSCs, clinical outcomes, and complication rate...
April 30, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/29713634/cell-based-therapies-for-joint-disease-in-veterinary-medicine-what-we-have-learned-and-what-we-need-to-know
#16
REVIEW
Sophie Helen Bogers
Biological cell-based therapies for the treatment of joint disease in veterinary patients include autologous-conditioned serum, platelet-rich plasma, and expanded or non-expanded mesenchymal stem cell products. This narrative review outlines the processing and known mechanism of action of these therapies and reviews current preclinical and clinical efficacy in joint disease in the context of the processing type and study design. The significance of variation for biological activity and consequently regulatory approval is also discussed...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29704190/tissue-engineering-in-osteoarthritis-current-status-and-prospect-of-mesenchymal-stem-cell-therapy
#17
Gun-Il Im
Osteoarthritis (OA) is the most common form of arthritis. Over the last 20 years, attempts have been made to regenerate articular cartilage to overcome the limitations of conventional treatments. As OA is generally associated with larger and diffuse involvement of articular surfaces and alteration of joint homeostasis, a tissue engineering approach for cartilage regeneration is more difficult than in simple chondral defects. Autologous and allogeneic mesenchymal stem cells (MSCs) have rapidly emerged as investigational products for cartilage regeneration...
April 27, 2018: BioDrugs: Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy
https://www.readbyqxmd.com/read/29692195/exosomes-and-stem-cells-in-degenerative-disease-diagnosis-and-therapy
#18
Yu-Hsun Chang, Kung-Chi Wu, Horng-Jyh Harn, Shinn-Zong Lin, Dah-Ching Ding
Stroke can cause death and disability, resulting in a huge burden on society. Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by motor dysfunction. Osteoarthritis (OA) is a progressive degenerative joint disease characterized by cartilage destruction and osteophyte formation in the joints. Stem cell therapy may provide a biological treatment alternative to traditional pharmacological therapy. Mesenchymal stem cells (MSCs) are preferred because of their differentiation ability and possible derivation from many adult tissues...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29687871/cell-therapy-of-a-knee-osteoarthritis-rat-model-using-precartilaginous-stem-cells
#19
M-P Fan, M Si, B-J Li, G-H Hu, Y Hou, W Yang, L Liu, B Tang, L Nie
OBJECTIVE: To explore the effect and mechanism of precartilaginous stem cells (PSCs) engraftment-inducing tissue repair in a knee osteoarthritis (OA) rat model. MATERIALS AND METHODS: Knee osteoarthritis (OA) model was constructed in Sprague Dawley (SD) rats by partial removal of the medial meniscus of the right knee. PSCs were engrafted by injecting precartilaginous stem cells (PSCs) into the right knee cavity. At 4 and 8 weeks after model construction, the serum levels of interleukine (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 were assessed using enzyme-linked immunosorbent assay (ELISA)...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29684624/component-effect-of-stem-cell-loaded-thermosensitive-polypeptide-hydrogels-on-cartilage-repair
#20
He Liu, Yilong Cheng, Jinjin Chen, Fei Chang, Jincheng Wang, Jianxun Ding, Xuesi Chen
Biophysical properties of the desired biomimetic scaffolds, such as porosity and elasticity, have been proven associated with the efficacy of cartilage regeneration. In this work, the copolymers of poly(L-alanine)-block-poly(ethylene glycol)-block-poly(L-alanine) (PA-PEG-PA) and poly(L-alanine-co-L-phenylalanine)-block-poly(ethylene glycol)-block-poly(L-alanine-co-L-phenylalanine) (PAF-PEG-PAF) with different ratios of alanine to phenylalanine were synthesized. The introduction of a hydrophobic amino acid, phenylalanine, into polyalanine-based thermosensitive hydrogel leaded to the enhanced gelation behaviors and upregulated mechanical properties...
April 20, 2018: Acta Biomaterialia
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