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https://www.readbyqxmd.com/read/29035889/inhibition-of-suicidal-erythrocyte-death-by-volasertib
#1
Abdulla Al Mamun Bhuyan, A K M Ashiqul Haque, Itishri Sahu, Hang Cao, Michael S D Kormann, Florian Lang
BACKGROUND/AIMS: The Polo-like kinase 1 (Plk1) inhibitor volasertib is used in the treatment of malignancy. Volasertib is partially effective by triggering suicidal death or apoptosis of tumor cells. Similar to apoptosis of nucleated cells, erythrocytes may enter suicidal cell death or eryptosis, which is characterized by cell membrane scrambling with phosphatidylserine translocation to the cell surface and by cell shrinkage. Stimulators of eryptosis include energy depletion, hyperosmotic shock, oxidative stress and excessive increase of cytosolic Ca2+ activity ([Ca2+]i)...
October 16, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29035162/effects-of-osmolytes-on-stable-uucg-tetraloops-and-their-preference-for-a-cg-closing-base-pair
#2
Michelle E Whittum, Joshua M Blose
Osmolytes have the potential to affect the stability of secondary structure motifs and alter preferences for conserved nucleic acid sequences in the cell. To contribute to the understanding of the in vivo function of RNA we observed the effects of different classes of osmolytes on the UNCG tetraloop motif. UNCG tetraloops are the most common and stable of the RNA tetraloops and are nucleation sites for RNA folding. They also have a significant thermodynamic preference for a CG closing base pair. The thermal denaturation of model hairpins containing UUCG loops was monitored using UV-Vis spectroscopy in the presence of osmolytes with different chemical properties...
October 16, 2017: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/29033821/certolizumab-pegol-induced-folliculitis-like-lichenoid-sarcoidosis-in-a-patient-with-rheumatoid-arthritis
#3
Hiroyuki Sakai, Wakana Nomura, Motoshi Sugawara
Anti-tumor necrosis factor α (TNF-α) biologic agents are used for treating refractory sarcoidosis. However, sarcoidosis-like epithelioid cell granulomas may develop during anti-TNF-α treatment. A 63-year-old man suffering from rheumatoid arthritis was treated with oral methotrexate and methylprednisolone for 4 years. He subsequently started biweekly subcutaneous injections of certolizumab pegol. Three months later, light red follicular papules developed on his chest and they spread over the trunk and bilateral upper arms...
September 2017: Case Reports in Dermatology
https://www.readbyqxmd.com/read/29033329/nanoscale-visualization-of-biomineral-formation-in-coral-proto-polyps
#4
Tali Mass, Jeana L Drake, John M Heddleston, Paul G Falkowski
Calcium carbonate platforms produced by reef-building stony corals over geologic time are pervasive features around the world [1]; however, the mechanism by which these organisms produce the mineral is poorly understood (see review by [2]). It is generally assumed that stony corals precipitate calcium carbonate extracellularly as aragonite in a calcifying medium between the calicoblastic ectoderm and pre-existing skeleton, separated from the overlying seawater [2]. The calicoblastic ectoderm produces extracellular matrix (ECM) proteins, secreted to the calcifying medium [3-6], which appear to provide the nucleation, alteration, elongation, and inhibition mechanisms of the biomineral [7] and remain occluded and preserved in the skeleton [8-10]...
October 11, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29032357/actin-assembly-mechanisms-at-a-glance
#5
REVIEW
Klemens Rottner, Jan Faix, Sven Bogdan, Stefan Linder, Eugen Kerkhoff
The actin cytoskeleton and associated motor proteins provide the driving forces for establishing the astonishing morphological diversity and dynamics of mammalian cells. Aside from functions in protruding and contracting cell membranes for motility, differentiation or cell division, the actin cytoskeleton provides forces to shape and move intracellular membranes of organelles and vesicles. To establish the many different actin assembly functions required in time and space, actin nucleators are targeted to specific subcellular compartments, thereby restricting the generation of specific actin filament structures to those sites...
October 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29031897/smart-electrical-bi-layers-lipopeptides-novel-peptidic-chains-like-zigzag-map-esterified-with-phospho-glyceride-as-mono-layer-moieties-capable-in-forming-a-meso-sphere-envelop-with-scaffold-ability-to-cellular-impurities
#6
REVIEW
Sami Saadi, Nazamid Saari, Abdulkarim Sabo Mohammed, Hasanah Mohd Ghazali, Farooq Anwar
Cell impurities are an emerging nucleating molecular barriers having the capability in disordering the metabolic chain reactions of proteolysis, glycolysis and lipolysis. Their massive effects induced by copolymer crystal growth in compaction with metal and mineral transients are extended as well as in damaging DNA and mRNA structure motif and other molecular assembly e.g. histones structure unites. Their polycrystalline packing modes, polydispersity and their tendency to surface and interface adhesion prompted us in structuring scaffold biomaterials enriched with biopeptides, layered by phospho-glycerides ester-forms...
October 11, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29027017/palladium-bionanoparticles-production-from-acidic-pd-ii-solutions-and-spent-catalyst-leachate-using-acidophilic-fe-iii-reducing-bacteria
#7
Naoko Okibe, Daisuke Nakayama, Takahiro Matsumoto
The acidophilic, Fe(III)-reducing heterotrophic bacteria Acidocella aromatica PFBC(T) and Acidiphilium cryptum SJH were utilized to produce palladium (Pd) bionanoparticles via a simple 1-step microbiological reaction. Monosaccharide (or intracellular NADH)-dependent reactions lead to visualization of intra/extra-cellular enzymatic Pd(0) nucleation. Formic acid-dependent reactions proceeded via the first slow Pd(0) nucleation phase and the following autocatalytic Pd(II) reduction phase regardless of the presence or viability of the cells...
October 12, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29026530/recent-advances-in-pericentriolar-material-organization-ordered-layers-and-scaffolding-gels
#8
REVIEW
Andrew M Fry, Josephina Sampson, Caroline Shak, Sue Shackleton
The centrosome is an unusual organelle that lacks a surrounding membrane, raising the question of what limits its size and shape. Moreover, while electron microscopy (EM) has provided a detailed view of centriole architecture, there has been limited understanding of how the second major component of centrosomes, the pericentriolar material (PCM), is organized. Here, we summarize exciting recent findings from super-resolution fluorescence imaging, structural biology, and biochemical reconstitution that together reveal the presence of ordered layers and complex gel-like scaffolds in the PCM...
2017: F1000Research
https://www.readbyqxmd.com/read/29025644/polylactic-acid-based-porous-scaffolds-doped-with-calcium-silicate-and-dicalcium-phosphate-dihydrate-designed-for-biomedical-application
#9
Maria Giovanna Gandolfi, Fausto Zamparini, Micaela Degli Esposti, Federica Chiellini, Conrado Aparicio, Fabio Fava, Paola Fabbri, Paola Taddei, Carlo Prati
Polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD) and/or hydraulic calcium silicate (CaSi) have been used to prepare highly-porous scaffolds by thermally induced phase separation technique (TIPS). Three experimental mineral-doped formulations were prepared (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Pure PLA scaffolds constituted the control group. Scaffolds were tested for their chemical-physical and biological properties, namely calcium release, alkalinizing activity, surface microchemistry and micromorphology by ESEM, apatite-forming ability by EDX, micro-Raman and IR spectroscopy, thermal properties by differential scanning calorimetry, mechanical properties by quasi-static parallel-plates compression testing, porosity by a standard water-absorption method and direct-contact cytotoxicity...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29024138/safranal-inhibits-hela-cell-viability-by-perturbing-the-reassembly-potential-of-microtubules
#10
Sanith Cheriyamundath, Sinjan Choudhary, Manu Lopus
Saffron, a spice from Crocus sativus, has been known for its health benefits and medicinal properties. Safranal is a component of saffron and is known for its antioxidant and anticancer properties. In this study, we elucidated a possible tubulin-targeted antiproliferative mechanism of action of safranal. In vitro, the compound perturbed secondary structure of tubulin without altering net microtubule polymer mass. It inhibited HeLa cell viability in a concentration-dependent manner, with minimal damage to cellular microtubules...
October 11, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29021250/electrostatic-lipid-protein-interactions-sequester-the-curli-amyloid-fold-on-the-lipopolysaccharide-membrane-surface
#11
Hema M Swasthi, Samrat Mukhopadhyay
Curli is a functional amyloid protein in the extracellular matrix of enteric Gram-negative bacteria. Curli is assembled at the cell surface and consists of CsgA, the major subunit of curli, and a membrane-associated nucleator protein, CsgB. Oligomeric intermediates that accumulate during the lag phase of amyloidogenesis are generally toxic, but the underlying mechanism by which bacterial cells overcome this toxicity during curli assembly at the surface remains elusive. Here, we elucidated the mechanism of curli amyloidogenesis and provide molecular insights into the strategy by which bacteria can potentially bypass the detrimental consequences of toxic amyloid intermediates...
October 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29017100/enhanced-formation-of-hydroxyapatites-in-gelatin-imogolite-macroporous-hydrogels
#12
Rita Gelli, Stefano Del Buffa, Paolo Tempesti, Massimo Bonini, Francesca Ridi, Piero Baglioni
HYPOTHESIS: Gelatin is widely investigated for the fabrication of synthetic scaffolds in bone tissue engineering. Practical limitations to its use are mainly due to the fast dissolution rate in physiological conditions and to the lack of pores with suitable dimensions for cell permeation. The aim of this work is to exploit imogolite clays as nucleation sites for the growth of calcium phosphates in gelatin-based hydrogels and to take advantage of a cryogenic treatment to obtain pores of ∼100µm...
September 28, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28993511/cell-cycle-dependent-tumor-engraftment-and-migration-are-enabled-by-aurora-a
#13
Tony L H Chu, Marisa Connell, Lixin Zhou, Zhengcheng He, Jennifer R Won, Seyed M Rahavi, Helen Chen, Pooja Mohan, Oksana Nemirovsky, Abbas Fotovati, Miguel Angel Pujana, Gregor Sd Reid, Torsten O Nielsen, Nelly Pante, Christopher A Maxwell
Cell cycle progression and the acquisition of a migratory phenotype are hallmarks of human carcinoma cells that are perceived as independent processes but may be interconnected by molecular pathways that control microtubule nucleation at centrosomes. Here, cell cycle progression dramatically impacts the engraftment kinetics of 4T1-luciferase2 breast cancer cells in immunocompetent BALB/c or immunocompromised NOD-SCID gamma (NSG) mice. Multi-parameter imaging of wound closure assays was used to track cell cycle progression, cell migration, and associated phenotypes in epithelial cells or carcinoma cells expressing a fluorescence ubiquitin cell cycle indicator...
October 9, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28990755/tunable-and-linker-free-nanogaps-in-core-shell-plasmonic-nanorods-for-selective-and-quantitative-detection-of-circulating-tumor-cells-by-sers
#14
Yang Zhang, Peng Yang, Habeeb Muhammed Madathumpady Abubaker, Shahad K Alsaiari, Basem Moosa, Abdulaziz AlMalik, Anjli Kumar, Emilie Ringe, Niveen M Khashab
Controlling the size, number, and shape of nanogaps in plasmonic nanostructures is of significant importance for the development of novel quantum plasmonic devices and quantitative sensing techniques such as surface-enhanced Raman scattering (SERS). Here, we introduce a new synthetic method based on coordination interactions and galvanic replacement to prepare core-shell plasmonic nanorods with tunable enclosed nanogaps. Decorating Au nanorods with Raman reporters that strongly coordinate Ag+ ions (e.g., 4-mercaptopyridine) afforded uniform nucleation sites to form a sacrificial Ag shell...
October 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28990291/performance-evaluation-of-the-automated-nucleated-red-blood-cell-count-of-five-commercial-hematological-analyzers
#15
G Da Rin, M Vidali, F Balboni, A Benegiamo, M Borin, M L Ciardelli, F Dima, A Di Fabio, A Fanelli, F Fiorini, S Francione, L Germagnoli, M Gioia, T Lari, M Lorubbio, A Marini, A Papa, M Seghezzi, L Solarino, S Pipitone, E Tilocca, S Buoro
INTRODUCTION: Recent automated hematology analyzers (HAs) can identify and report nucleated red blood cells (NRBC) count as a separate population out of white blood cells (WBC). The aim of this study was to investigate the analytical performances of NRBC enumeration on five top of the range HAs. METHODS: We evaluated the within-run and between-day precision, limit of blank (LoB), limit of detection (LoD), and limit of quantitation (LoQ) of XE-2100 and XN-module (Sysmex), ADVIA 2120i (Siemens), BC-6800 (Mindray), and UniCel DxH 800 (Beckman Coulter)...
October 8, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28986300/echogenicity-enhancement-by-end-fluorinated-polylactide-perfluorohexane-nanocapsules-towards-ultrasound-activable-nanosystems
#16
Guilherme Picheth, Sophie Houvenagel, Camille Dejean, Olivier Couture, Rilton Alves de Freitas, Laurence Moine, Nicolas Tsapis
Polylactide (PLA) polymers containing five distinct lengths of fluorinated (from C3F7 to C13F27) and non-fluorinated (C6H13) end-groups were successfully synthesized by ring-opening polymerization of D,L-lactide. Fluorination was expected to increase the encapsulation efficiency of perfluorohexane (PFH). 150 nm nanocapsules were obtained and (19)F nuclear magnetic resonance revealed that nanocapsules formulated with fluorinated polymers increased by 2-fold the encapsulation efficiency of PFH compared with non-fluorinated derivatives, without any effect of fluorine chain length...
October 3, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28983581/bioinformatics-analysis-of-fibroblasts-exposed-to-tgf%C3%A2-%C3%AE-at-the-early-proliferation-phase-of-wound-repair
#17
Bobin Mi, Guohui Liu, Wu Zhou, Huijuan Lv, Kun Zha, Yi Liu, Qipeng Wu, Jing Liu
The aim of the current study was to identify gene signatures during the early proliferation stage of wound repair and the effect of TGF‑β on fibroblasts and reveal their potential mechanisms. The gene expression profiles of GSE79621 and GSE27165 were obtained from GEO database. Differentially expressed genes (DEGs) were identified using Morpheus and co‑expressed DEGs were selected using Venn Diagram. Gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs were performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) online tool...
September 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28983304/acetolactate-synthase-inhibiting-gametocide-amidosulfuron-causes-chloroplast-destruction-tissue-autophagy-and-elevation-of-ethylene-release-in-rapeseed
#18
Xi-Qiong Liu, Cheng-Yu Yu, Jun-Gang Dong, Sheng-Wu Hu, Ai-Xia Xu
Background: Acetolactate synthase (ALS)-inhibiting herbicides amidosulfuron (Hoestar) is an efficient gametocide that can induce male sterility in rapeseed (Brassica napus L.). We conducted an integrated study of cytological, transcriptomic, and physiological analysis to decipher the gametocidal effect of amidosulfuron. Results: In the first several days after exposure to amidosulfuron at a gametocidal dose of ca. 1 μg per plant, the plants showed the earliest symptoms including short retard of raceme elongation, slight chlorosis on leaf, and decrease of photosynthesis rate...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28982292/promotion-of-myogenic-maturation-by-timely-application-of-electric-field-along-the-topographical-alignment
#19
Ung Hyun Ko, Suk-Hee Park, Hyunseung Bang, Mina Kim, Hyunjun Shin, Jennifer Shin
Engineered muscular substitutes can restore the impaired muscle functions when integrated properly into the host tissue. To generate functional muscles with sufficient contractility at the site of transplant, the in vitro construction of fully differentiated muscle fibers would be desired. Many previous reports have identified either topographical alignment or electrical stimulation as an effective tool to promote myogenic differentiation. However, optimization of spatial and temporal arrangement of these two physical cues for better differentiation and maturation of skeletal muscles has not been investigated...
October 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28980799/silica-assisted-nucleation-of-polymer-foam-cells-with-nanoscopic-dimensions-impact-of-particle-size-line-tension-and-surface-functionality
#20
Shanqiu Liu, Rik Eijkelenkamp, Joost Duvigneau, G Julius Vancso
Core-shell nanoparticles consisting of silica as core and surface grafted polydimethylsiloxane (PDMS) as shell with different diameters were prepared and used as heterogeneous nucleation agents to obtain CO2 blown poly(methyl methacrylate) (PMMA) nanocomposite foams. PDMS was selected as shell material as it possesses a low surface energy and high CO2-philicity. The successful synthesis of core-shell nanoparticles was confirmed by Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA) and transmission electron microscopy (TEM)...
October 5, 2017: ACS Applied Materials & Interfaces
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