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https://www.readbyqxmd.com/read/29753846/role-of-hnf1%C3%AE-in-the-differential-diagnosis-from-other-germ-cell-tumors
#1
Anne-Laure Rougemont, Jean-Christophe Tille
Identification of the yolk sac tumor (YST) component in germ cell tumors (GCT) may prove challenging, and highly sensitive and specific immunohistochemical markers are still lacking. Preliminary data from the literature suggest that HNF1β may represent a sensitive marker of YST. The specificity of HNF1β has not been addressed in GCT. A cohort of 49 YST specimens from 45 patients was designed, occurring either as pure tumors, or as a component of a mixed GCT. Immunohistochemistry was conducted on whole tumor sections using HNF1β...
May 10, 2018: Human Pathology
https://www.readbyqxmd.com/read/29751880/update-on-ancillary-testing-in-the-evaluation-of-high-grade-liver-tumors
#2
REVIEW
Anne Koehne de Gonzalez, Stephen M Lagana
Tissue diagnosis is the gold standard for mass lesions of the liver, but needle core biopsies may sometimes prove challenging. Presented here is a review of a panel of immunohistochemical stains, including hepatocyte in paraffin 1, arginase-1, polyclonal carcinoembryonic antigen, CD10, bile salt export pump, glypican-3, as well as in situ hybridization for albumin RNA, to establish hepatocellular origin in cases in which hepatocellular carcinoma is suspected but the sample is limited or the morphology is challenging, as it may be with cases of scirrhous, fibrolamellar carcinoma, intrahepatic cholangiocarcinoma, and combined hepatocellular-cholangiocarcinoma...
June 2018: Surgical Pathology Clinics
https://www.readbyqxmd.com/read/29751042/next-generation-sequencing-analysis-for-gastric-adenocarcinoma-with-enteroblastic-differentiation-emphasis-on-the-relationship-with-hepatoid-adenocarcinoma
#3
Yoichi Akazawa, Tsuyoshi Saito, Takuo Hayashi, Yuka Yanai, Sho Tsuyama, Keisuke Akaike, Yoshiyuki Suehara, Fumiyuki Takahashi, Kazuya Takamochi, Hiroya Ueyama, Takashi Murakami, Sumio Watanabe, Akihito Nagahara, Takashi Yao
Histologically tubulo-papillary structures with glycogen-rich clear cytoplasm in gastric adenocarcinoma with enteroblastic differentiation (GAED) are well-known, but a solid growth pattern can also be observed as a minor component. In contrast, hepatoid adenocarcinoma (HAC) of the stomach shows many overlapping features, including solid pattern and alpha-fetoprotein expression. In this study, we employed next-generation sequencing (NGS) to establish a molecular/clinicopathological concept of GAED and clarify whether these two tumors should be grouped together in one category...
May 8, 2018: Human Pathology
https://www.readbyqxmd.com/read/29708315/glypican-3-and-melanoma-antigen-genes-1-and-3-as-tumor-markers-for-hepatocellular-carcinoma
#4
Nadia M El-Wahab, Hebat-Allah G Rashed, Wafaa T El-Sherif, Sohair K Sayed, Mohamed O Abd-Elmalek, Abeer Refaiy, Hussein Fakhry, Abeer A Mokhtar
Hepatocellular carcinoma (HCC) is the commonest liver cancer; its incidence and prevalence are continuously increased. Glypican3 (GPC3), melanoma antigen-1, 3 genes (MAGE1 and 3) are tumor markers used in HCC. We evaluated their role in HCC detection and assessed their relation to tumor parameters. Three groups, HCC group, liver cirrhosis group and a control group were studied. AFP, GPC3, and MAGE1 and 3 mRNA were determined in all study subjects. Tissue GPC3 was examined in patients with HCC only. Serum AFP and GPC3 were elevated in HCC group compared to other groups (P < 0...
June 2017: Egyptian Journal of Immunology
https://www.readbyqxmd.com/read/29675356/-in-vivo-fluorescence-imaging-of-hepatocellular-carcinoma-using-a-novel-gpc3-specific-aptamer-probe
#5
Menglong Zhao, Lili Dong, Zhuang Liu, Shuohui Yang, Weizhong Wu, Jiang Lin
Background: Glypican-3 (GPC3) is highly expressed in most of the hepatocellular carcinomas (HCCs), even in small HCCs. It may be used as a potential biomarker for early detection of HCC. The aptamer is a promising targeting agent with unique advantages over antibody. This study was to introduce a novel GPC3 specific aptamer (AP613-1), to verify its specific binding property in vitro , and to evaluate its targeting efficiency in vivo by performing near-infrared (NIR) fluorescence imaging on an HCC xenograft model...
March 2018: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/29669302/cytotoxic-and-partial-hepatoprotective-activity-of-sodium-ascorbate-against-hepatocellular-carcinoma-through-inhibition-of-sulfatase-2-in-vivo-and-in-vitro
#6
Abdullah Alyoussef, Mohammed M H Al-Gayyar
Hepatocellular carcinoma (HCC) is characterized by elevation in the activity of sulfatase-2, an extracellular enzyme that catalyzes removal of 6-O-sulfate groups from heparan sulfate. Therefore, we conducted this study to investigate the cytotoxic activity of the strong water-soluble antioxidant, sodium ascorbate, against HCC both in vivo and in vitro. Sodium ascorbate enhanced animal survival in vivo and reduced HepG2 cells survival. The protein levels of heparan sulfate proteoglycans (HSPGs), insulin like growth factor (IGF)-2, sulfatase-2 and glypican-3 were assessed...
April 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29624843/a-novel-multimarker-assay-for-the-phenotypic-profiling-of-circulating-tumor-cells-in-hepatocellular-carcinoma
#7
Colin M Court, Shuang Hou, Paul Winograd, Nicholas H Segel, Qingyu Wilda Li, Yazhen Zhu, Saeed Sadeghi, Richard S Finn, Ekambaram Ganapathy, Min Song, Samuel W French, Bita V Naini, Shonan Sho, Fady M Kaldas, Ronald W Busuttil, James S Tomlinson, Hsian-Rong Tseng, Vatche G Agopian
Current clinicopathologic staging systems and serum biomarkers poorly discriminate tumor biology in hepatocellular carcinoma (HCC), with high recurrence rates following curative-intent surgical resection and liver transplantation (LT). Identification of accurate biomarkers for improved prognostication and treatment selection is a critical unmet need. We sought to develop a novel "liquid-biopsy" assay capable of detecting HCC circulating tumor cells (CTCs), and characterizing phenotypic subpopulations with prognostic significance...
April 6, 2018: Liver Transplantation
https://www.readbyqxmd.com/read/29623600/significance-of-glypican-3-in-early-detection-of-hepatocellular-carcinoma-in-cirrhotic-patients
#8
Ahmed M Tahon, Magdy Z El-Ghanam, Samy Zaky, Tarek Mostafa Emran, Ali M Bersy, Fathiya El-Raey, Elsayed A Z, Amr M El Kharsawy, Dina Johar
BACKGROUND: Egypt has high incidence of hepatocellular carcinoma (HCC). This is due to wide spread of hepatitis C virus (HCV) infection which is responsible for most of the cases of liver cirrhosis. The major diagnostic techniques for HCC include serum markers and various imaging modalities. Glypican 3 (GPC3) protein is highly expressed in HCC, but not in normal liver tissue. The significance of GPC3 as a predictor or diagnostic tool for human tumors other than HCC is unclear. AIM: To quantitatively assess the role of GPC3 in diagnosis of HCC in comparison to α-fetoprotein (AFP), ultrasonography (US), and computerized tomography (CT)...
April 6, 2018: Journal of Gastrointestinal Cancer
https://www.readbyqxmd.com/read/29605994/slow-off-rate-modified-aptamer-somamer-as-a-novel-reagent-in-immunoassay-development-for-accurate-soluble-glypican-3-quantification-in-clinical-samples
#9
Jia Duo, Camelia Chiriac, Richard Y-C Huang, John Timothy Mehl, Guodong Chen, Adrienne A Tymiak, Peter Sabbatini, Renuka Pillutla, Yan J Zhang
Accurate quantification of soluble glypican-3 in clinical samples using immunoassays is challenging due to the lack of appropriate antibody reagents to provide a full spectrum measurement of all potential soluble glypican-3 fragments in vivo. Glypican-3 SOMAmer (Slow Off-Rate Modified Aptamer) is a novel reagent which binds, with high affinity, to a far distinct epitope of glypican-3 when compared to all available antibody reagents generated in house. This paper describes an integrated analytical approach to rational selection of key reagents based on molecular characterization by epitope mapping, with the focus on our work using a SOMAmer as a new reagent to address development challenges with traditional antibody reagents for the soluble glypican-3 immunoassay...
March 31, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29582639/diagnostic-value-of-glypican-3-for-hepatocellular-carcinomas
#10
Sherif El-Saadany, Taher El-Demerdash, Amal Helmy, Wael Wahid Mayah, Boshra El-Sayed Hussein, Mohammed Hassanien, Nehal Elmashad, Mahmoud Ali Fouad, Eman Ahmed Basha
Background: Hepatocellular carcinoma (HCC) is a common and dangerous malignancy in many parts of the world, and especially in Egypt. Early diagnosis is the most important step in successful HCC management. However most cases are detected at late stage making effective intervention impossible. Aim: The aim of this study was to evaluate the potential of Glypican-3 (GPC-3) to aid in diagnosis of HCC, especially in patients with low serum alpha-fetoprotein (AFP). Subjects and methods: Serum GPC-3 was assessed by flow-cytometry and serum AFP by enzyme-linked immunosorbent assay (ELISA) in 40 HCC patients with AFP< 400ug\l...
March 27, 2018: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29577937/hepatitis-c-virus-hcv-mimics-effects-of-glypican-3-gpc3-on-cd81-and-promotes-development-of-hepatocellular-carcinomas-via-activation-of-hippo-pathway-in-hepatocytes
#11
Yuhua Xue, Wendy M Mars, William Bowen, Aatur D Singhi, John Stoops, George K Michalopoulos
Glypican 3 (GPC3) is over-expressed in hepatocellular carcinomas (HCC). GPC3 binds to CD81. Forced expression of CD81 in a GPC3 expressing HCC cell line caused activation of Hippo, decrease in Ezrin phosphorylation, and decrease in Yap. CD81 is also associated with HCV entry into hepatocytes. Activation of CD81 by agonistic antibody causes activation of spleen kinase (Syk) and phosphorylation of Ezrin, a regulator of Hippo pathway. In cultures of normal hepatocytes, CD81 agonistic antibody led to enhanced phosphorylation of Ezrin and increase in nuclear Yap...
March 22, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29569872/concomitant-use-of-heat-shock-protein-70-glutamine-synthetase-and-glypican-3-is-useful-in-diagnosis-of-hbv-related-hepatocellular-carcinoma-with-higher-specificity-and-sensitivity
#12
Bita Moudi, Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb, Seyed-Moayed Alavian, Kamran B Lankarani, Parisa Farrokh, Jens Randel Nyengaard
Hepatocellular carcinoma is the third leading cause of cancer-related death worldwide and late diagnosis is the main cause of death in HCC patients. In this study expression patterns of HSP70, GPC3 and GS and their relationships with pathogenesis of HCC in Iranian patients were investigated. The expression of HSP70, GPC3 and GS were determined by immunohistochemistry and quantitative real-time PCR (q-PCR) methods, using 121 cases from patients with HBV alone, HCC without HBV, HBV+HCC and 30 normal tissues as control group...
January 29, 2018: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29567548/analysis-of-proteoglycan-expression-in-human-dental-pulp
#13
Ana Carla Raphaelli Nahás-Scocate, Gabrielle Ferrante Alves de Moraes, Helena Bonciani Nader, Carolina Meloni Vicente, Leny Toma
Proteoglycans are glycosylated proteins which have covalently attached highly anionic glycosaminoglycans. They can be located on the extracellular matrix, cell membrane or intracellular granules. To date, few studies have reported the presence of proteoglycans in human dental pulp. OBJECTIVE: The aim of this study was, therefore, to analyze the expression of lumican, versican and glypican proteoglycans in deciduous and permanent human dental pulp by real-time polymerase chain reaction (q-PCR) and immunofluorescence...
March 10, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29563582/evaluation-of-the-glypican-3-promoter-for-transcriptional-targeting-of-hepatocellular-carcinoma
#14
Bijay Dhungel, Slawomir Andrzejewski, Aparna Jayachandran, Ritu Shrestha, Charmaine A Ramlogan-Steel, Christopher J Layton, Jason C Steel
Hepatocellular carcinoma (HCC) is a major health problem as evidenced by its increasing incidence and high morbidity and mortality rates. Most patients with HCC have underlying liver disease and dysfunction which limits the current therapeutic options. Treatments that spare the liver and destroy the HCC are needed. Targeting transcriptional differences between HCC and liver cells may provide this therapeutic window. In this study, we examine the potential of the Glypican 3 (GPC3) promoter as a targeting strategy...
March 21, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29561231/polyclonal-antibody-production-against-rgpc3-and-their-application-in-diagnosis-of-hepatocellular-carcinoma
#15
Shenghao Wang, Muhammad Kalim, Keying Liang, Jinbiao Zhan
Glypican-3 (GPC3) is an integral membrane proteoglycan, which contains a core protein anchored to the cytoplasmic membrane through a glycosyl phosphatidylinositol linkage. The glypican-3 can regulate the signaling pathways, thereby enhances cell division, growth, and apoptosis in certain cell types. It is almost non-existent on the surface of the human normal cell membrane and highly expresses on the membrane of hepatocellular carcinoma (HCC) cells. It has been well established that GPC3 provide a useful diagnostic marker...
March 21, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29546798/metastatic-choriocarcinoma-masquerading-as-a-congenital-glabellar-hemangioma
#16
Steve Taylor, Kimberly Eisenstein, Vanessa Gildenstern, Harper Price, Pooja Hingorani, Apurvi Patel, Nathan Page, Smita Bailey, David Carpentieri
Infantile choriocarcinoma (ICC) is a rare, highly malignant form of gestational trophoblastic neoplasia. Rapid diagnosis and initiation of treatment are paramount in reaching a successful outcome. Patients with these tumors typically present with a triad of anemia, hepatomegaly, and precocious puberty. Cutaneous manifestations of ICC are extraordinarily rare with few documented cases. Here, we describe a male neonate who presented to our Dermatology clinic with a rapidly growing, markedly vascular glabellar mass associated with abnormal laboratory values suggestive of Kasabach-Merritt phenomenon...
January 1, 2018: Pediatric and Developmental Pathology
https://www.readbyqxmd.com/read/29535817/combining-expression-of-gpc3-in-tumors-and-cd16-on-nk-cells-from-peripheral-blood-to-identify-patients-responding-to-codrituzumab
#17
Gong Chen, Ya-Chi Chen, Bernhard Reis, Anton Belousov, Lori Jukofsky, Christine Rossin, Axel Muehlig, Chao Xu, Laurent Essioux, Toshihiko Ohtomo, Laura Di Laurenzio, Oscar Puig, Ray Lee
Background: Codrituzumab, a monoclonal antibody targeting an oncofetal protein glypican-3 (GPC3) expressed on cell surface of hepatocellular carcinoma (HCC) induces antibody-dependent cellular cytotoxicity (ADCC) and inhibits tumor growth in preclinical studies. Based on this mechanism, tumor GPC3 expression and CD16 expression on NK cells, which are the effector cells of ADCC, were investigated to correlate with codrituzumab's clinical efficacy in patients with advanced HCC. Results: Joint analyses of the two biomarkers revealed that both high levels of GPC3 and CD16 were required for patients to benefit from codrituzumab; lack of either one of them would lead to a loss of the therapeutic effect...
February 13, 2018: Oncotarget
https://www.readbyqxmd.com/read/29508107/i-124-codrituzumab-imaging-and-biodistribution-in-patients-with-hepatocellular-carcinoma
#18
Jorge A Carrasquillo, Joseph A O'Donoghue, Volkan Beylergil, Shutian Ruan, Neeta Pandit-Taskar, Steven M Larson, Peter M Smith-Jones, Serge K Lyashchenko, Norihisa Ohishi, Toshihiko Ohtomo, Ghassan K Abou-Alfa
BACKGROUND: I-124 codrituzumab (aka GC33), an antibody directed at Glypican 3, was evaluated in patients with hepatocellular carcinoma (HCC). Fourteen patients with HCC underwent baseline imaging with I-124 codrituzumab (~ 185 MBq, 10 mg). Seven of these patients undergoing sorafenib/immunotherapy with 2.5 or 5 mg/kg of cold codrituzumab had repeat imaging, with co-infusion of I-124 codrituzumab, as part of their immunotherapy treatment. Three patients who progressed while on sorafenib/immunotherapy were re-imaged after a 4-week washout period to assess for the presence of antigen...
March 5, 2018: EJNMMI Research
https://www.readbyqxmd.com/read/29479987/ellagic-acid-holds-promise-against-hepatocellular-carcinoma-in-an-experimental-model-mechanisms-of-action
#19
Asmaa M Zaazaa, Maha S Lokman, Aziza B Shalby, Hanaa H Ahmed, Sayed A El-Toumy
This research work was initiated to explore the efficacy of ellagic acid in mitigation of hepatocarcinogenesis in rats. Rats were distributed into 4 groups; negative control, hepatocellular carcinoma (HCC), doxorubicin and ellagic acid. Serum alpha-fetoprotein (AFP), glypican-3 (GPC-3), signal transducer and activator of transcription 3 (STAT3) and suppressors of cytokine signaling 3 (SOCS3) levels were assayed by ELISA. Immunohistochemical examination of hepatic VEGF expression was also conducted, along with histological procedures for examination of liver tissue sections...
February 26, 2018: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29429512/effect-of-human-umbilical-cord-blood-derived-cd34-hematopoietic-stem-cell-on-the-expression-of-wnt4-and-p53-genes-in-a-rat-model-of-hepatocellular-carcinoma
#20
Rania Naiem Sherif, Hussein Abdellatif, Noha Hazem, Neven A Ebrahim, Dalia Saleh, Gamal Shiha, Huda Eltahry, Kamal G Botros, Omar M Gabr
BACKGROUND AND AIM OF THE WORK: Hepatocellular carcinoma (HCC) is the most frequent primary liver malignancy. Chronic liver injuries as chronic hepatitis C and hepatitis B viruses, aflatoxins consumption and nonalcoholic fatty liver disease are well-established causes of HCC. HCC is associated with a series of molecular changes, as alternation in glypican-3, P53 expression and Wnt/β-catenin pathway. Hepatic cancer progenitor cells could contribute to HCC development. This research aimed to study the effectiveness of human CD34+ hematopoietic stem cell on Wnt4 and P53 genes expression, histopathological grading and hepatic progenitor cells percentage in HCC rat model...
February 2018: Tissue & Cell
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