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https://www.readbyqxmd.com/read/28932558/results-of-quantitative-chest-ct-in-chronic-pulmonary-graft-vs-host-disease-cgvhd-3-years-after-allogeneic-stem-cell-transplantation
#1
Christopher Kloth, Wolfgang M Thaiss, Jürgen Hetzel, Georg Bier, Stefan Wirths, Konstantin Nikolaou, Marius Horger
BACKGROUND: To quantify lung parenchymal changes in symptomatic patients with chronic pulmonary graft-versus-host disease 3 years after allogeneic stem cell transplantation (allo-SCT) by means of CT-densitometry (CTD) and to compare results with those of established pulmonary function tests (PFT). METHODS: The study group consisted of 26 patients with pulmonary cGvHD (19 males, 7 females; mean age, 49.29±15.89; range, 19-72 years). The diagnosis was based on clinical symptoms, PFT and chest-CT findings...
August 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28922726/chronic-arsenicosis-and-cadmium-exposure-in-wild-snowshoe-hares-lepus-americanus-breeding-near-yellowknife-northwest-territories-canada-part-2-manifestation-of-bone-abnormalities-and-osteoporosis
#2
S Amuno, A Al Kaissi, A Jamwal, S Niyogi, C E Quenneville
Various bone abnormalities, including osteoporosis, have been associated with chronic arsenic and cadmium exposure in experimental animal models, but information regarding the bone pathology of wild population of small mammals breeding in contaminated environment is limited. This present study was conducted to comparatively assess the prevalence and pattern of skeletal abnormalities in free ranging snowshoe hares inhabiting an area heavily contaminated by arsenic and other trace metals, near the vicinity of the abandoned Giant mine, and in a reference location approximately 20km from the city of Yellowknife, Northwest Territories, Canada...
September 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28919730/a-new-functional-method-to-choose-the-target-lobe-for-lung-volume-reduction-in-emphysema-comparison-with-the-conventional-densitometric-method
#3
Juergen Hetzel, Michael Boeckeler, Marius Horger, Ahmed Ehab, Christopher Kloth, Robert Wagner, Lutz Freitag, Dirk-Jan Slebos, Richard Alexander Lewis, Maik Haentschel
BACKGROUND: Lung volume reduction (LVR) improves breathing mechanics by reducing hyperinflation. Lobar selection usually focuses on choosing the most destroyed emphysematous lobes as seen on an inspiratory CT scan. However, it has never been shown to what extent these densitometric CT parameters predict the least deflation of an individual lobe during expiration. The addition of expiratory CT analysis allows measurement of the extent of lobar air trapping and could therefore provide additional functional information for choice of potential treatment targets...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28919366/prevalence-and-risk-factors-for-fragility-fracture-in-systemic-mastocytosis
#4
Yannick Degboé, Marine Eischen, Delphine Nigon, Pol-André Apoil, Claire Mailhol, Emilie Tournier, Camille Laurent, Katia Hanssens, Olivier Hermine, Carle Paul, Michel Laroche, Cristina Bulai-Livideanu
OBJECTIVES: Systemic mastocytosis (SM) is characterized by the accumulation of mast cells in tissues other than the skin. Bone involvement although frequent has not been thoroughly evaluated. Primary objective was to determine risk factors associated with fragility fractures (FF) in SM. Secondary objectives were to evaluate the ability of bone marrow tryptase (BMT) level to identify patients with FF, and to describe bone involvement in SM. METHODS: We analyzed retrospectively all consecutive patients seen in our expert center, with a diagnosis of SM according to the 2001 WHO criteria, and with complete bone assessment...
September 14, 2017: Bone
https://www.readbyqxmd.com/read/28913959/quantification-through-tlc-densitometric-analysis-repellency-and-anticholinesterase-activity-of-the-homemade-extract-of-indian-cloves
#5
Raphael S Affonso, Josélia A Lima, Bruno Lessa, João V O Caetano, Marcos T Obara, Andrea Nóbrega, Eugenie Nepominova, Kamil Musilek, Kamil Kuca, Gláucia Barbosa Candido Alves Slana, Tanos C C França
The rise of mosquito's transmitted diseases, like dengue, zika and chikungunya in Brazil in the last years has increased the concerns on protection against mosquito's bites. However, the prohibitive prices of the commercially available repellents for the majority of the Brazilian population, has provoked a search for cheaper solutions, like the use of the homemade ethanolic extract of Indian clove (Syzygium aromaticum L.) as repellent, which has been reported as quite efficient by the local press. In order to verify this, we performed here, the quantification of the main components of this extract through high-performance thin-layer chromatography (HPTLC)-densitometry and evaluated its efficiency as repellent, as well as the acetylcholinesterase (AChE) inhibition capacity...
September 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28889305/detection-of-cyclic-di-gmp-binding-proteins-utilizing-a-biotinylated-cyclic-di-gmp-pull-down-assay
#6
Jacob R Chambers, Karin Sauer
Cyclic di-GMP is an important regulatory messenger molecule that often directly interacts with proteins to alter function. It is therefore important to find potential c-di-GMP binding proteins and verify a direct interaction between them. Here, we describe a pull-down assay using biotinylated-c-di-GMP to capture a specific protein of interest followed by immunoblot analysis to determine relative protein abundance. This method also allows for addition of both specific and nonspecific competitors to determine specificity of c-di-GMP-protein binding...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28884215/effect-of-smoking-cessation-on-quantitative-computed-tomography-in-smokers-at-risk-in-a-lung-cancer-screening-population
#7
Bertram J Jobst, Oliver Weinheimer, Mila Trauth, Nikolaus Becker, Erna Motsch, Marie-Luise Groß, Jan Tremper, Stefan Delorme, Anke Eigentopf, Monika Eichinger, Hans-Ulrich Kauczor, Mark O Wielpütz
OBJECTIVE: To longitudinally evaluate effects of smoking cessation on quantitative CT in a lung cancer screening cohort of heavy smokers over 4 years. METHODS: After 4 years, low-dose chest CT was available for 314 long-term ex-smokers (ES), 404 continuous smokers (CS) and 39 recent quitters (RQ) who quitted smoking within 2 years after baseline CT. CT acquired at baseline and after 3 and 4 years was subjected to well-evaluated densitometry software, computing mean lung density (MLD) and 15th percentile of the lung density histogram (15TH)...
September 7, 2017: European Radiology
https://www.readbyqxmd.com/read/28872692/coomassie-staining-provides-routine-sub-femtomole-in-gel-detection-of-intact-proteoforms-expanding-opportunities-for-genuine-top-down-proteomics
#8
Nour Noaman, Prabhodh S Abbineni, Michael Withers, Jens R Coorssen
Modified colloidal Coomassie Brilliant Blue (cCBB) staining utilising a novel destain protocol and near-infrared fluorescence detection (nIRFD) rivals the in-gel protein detection sensitivity (DS) of SYPRO Ruby. However, established DS estimates are likely inaccurate in terms of 2DE-resolved proteoform 'spots' since DS is routinely measured from comparatively diffuse protein 'bands' following wide-well 1DE. Here, cCBB DS for 2DE-based proteomics was more accurately determined using narrow-well 1DE. As precise estimates of protein standard monomer concentrations are essential for accurate quantitation, coupling UV absorbance with gel-based purity assessments is described...
September 5, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28872519/changes-in-corneal-density-after-accelerated-corneal-collagen-cross-linking-with-different-irradiation-intensities-and-energy-exposures-1-year-follow-up
#9
Semra Akkaya Turhan, Ebru Toker
PURPOSE: To determine and compare the changes in corneal density after 2 different protocols of accelerated corneal collagen cross-linking (A-CXL) in patients with progressive keratoconus. METHODS: Two groups of eyes received A-CXL treatment; 20 eyes received A-CXL using continuous UVA light exposure at 9 mW/cm for 10 minutes with a total energy dose of 5.4 J/cm, and 24 eyes received A-CXL using continuous UVA light exposure at 30 mW/cm for 4 minutes with a total energy dose of 7...
August 31, 2017: Cornea
https://www.readbyqxmd.com/read/28872516/corneal-densitometry-in-healthy-corneas-and-its-correlation-with-endothelial-morphometry
#10
Kemal Tekin, Mehmet Ali Sekeroglu, Hasan Kiziltoprak, Pelin Yilmazbas
PURPOSE: To evaluate the correlations between corneal endothelial cell properties and corneal densitometry values and to investigate whether corneal densitometry may be used as an indicator of the health of corneal endothelium. METHODS: Two hundred fifty-three eyes were analyzed in this prospective cross-sectional study. Corneal densitometry was measured with densitometry software of Pentacam HR-Scheimpflug corneal topography over a 12-mm diameter of the cornea...
August 31, 2017: Cornea
https://www.readbyqxmd.com/read/28860112/risk-factors-for-falls-in-older-patients-with-cancer
#11
Xiaotao Zhang, Ming Sun, Suyu Liu, Cheuk Hong Leung, Linda Pang, Uday R Popat, Richard Champlin, Holly M Holmes, Vicente Valero, Colin P Dinney, Debu Tripathy, Beatrice J Edwards
OBJECTIVES: A rising number of patients with cancer are older adults (65 years of age and older), and this proportion will increase to 70% by the year 2020. Falls are a common condition in older adults. We sought to assess the prevalence and risk factors for falls in older patients with cancer. METHODS: This is a single-site, retrospective cohort study. Patients who were receiving cancer care underwent a comprehensive geriatric assessments, including cognitive, functional, nutritional, physical, falls in the prior 6 months and comorbidity assessment...
August 31, 2017: BMJ Supportive & Palliative Care
https://www.readbyqxmd.com/read/28853593/changes-in-bone-turnover-markers-in-primary-hyperparathyroidism-and-response-to-surgery
#12
P Rajeev, A Movseysan, A Baharani
Introduction Involvement of the bone is common in primary hyperparathyroidism. The aim of the study was to assess bone turnover markers in response to surgery for primary hyperparathyroidism. Methods This was a retrospective study of patients diagnosed and treated for parathyroid disease between 2005 and 2012. Interventions studied were surgery and medical treatment. The main outcome measures studied were serum levels of calcium, intact parathyroid hormone (iPTH), bone-specific alkaline phosphatase, N-terminal cross-linking propeptide of type 1 procollagen (P1NP) and C-terminal cross-linking telopeptides of type I collagen (CTX), both pre- and postoperatively at 6 months and 1 year; bone mineral density (at the spine and hip assessed by dual-energy x-ray absorptiometry after 1 year of treatment...
September 2017: Annals of the Royal College of Surgeons of England
https://www.readbyqxmd.com/read/28840668/characterization-of-a-low-expression-haplotype-in-canine-glutathione-s-transferase-gstt1-and-its-prevalence-in-golden-retrievers
#13
S Craft, J Ekena, B Mayer, D H Thamm, C Saba, R Chun, L A Trepanier
Glutathione S-transferase-theta (GSTT1) is a carcinogen detoxification enzyme, and low activity variants are associated with lymphoma in humans. We recently found a variant in the 3' untranslated region (UTR) of canine GSTT1, *101_102insT, which was predicted to change miRNA binding and was found in 5 of 17 golden retriever (GR) dogs with lymphoma but none of 14 healthy GRs. The aim of this study was to determine whether this variant led to decreased GSTT1 expression and was a discernible risk factor for lymphoma within the GR breed...
August 25, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/28837603/proteomic-analysis-of-protein-phosphatase-z1-from-candida-albicans
#14
Bernadett Márkus, Krisztina Szabó, Walter P Pfliegler, Katalin Petrényi, Enikő Boros, István Pócsi, József Tőzsér, Éva Csősz, Viktor Dombrádi
Protein phosphatase Z is a "novel type" fungus specific serine/threonine protein phosphatase. Previously our research group identified the CaPPZ1 gene in the opportunistic pathogen Candida albicans and reported that the gene deletion had several important physiological consequences. In order to reveal the protein targets and the associated mechanisms behind the functions of the phosphatase a proteomic method was adopted for the comparison of the cappz1 deletion mutant and the genetically matching QMY23 control strain...
2017: PloS One
https://www.readbyqxmd.com/read/28837529/corneal-tomographic-changes-after-uv-cross-linking-for-corneal-ectasia-1-year-results
#15
Zainab Baksoellah, Itay Lavy, Lamis Baydoun, Hilde C M Hooijmaijers, Korine van Dijk, Gerrit R J Melles
PURPOSE: To evaluate changes in maximum keratometry (Kmax), corneal higher-order aberrations (HOAs), and densitometry (backscattered light) up to 1 year after UV cross-linking and their possible relation with changes in the visual outcome. METHODS: Retrospective cohort study on 18 eyes of 16 patients, who underwent UV cross-linking after the Dresden protocol for progressive keratoconus or ectasia after laser-assisted in situ keratomileusis. Corrected distance visual acuity (CDVA), Scheimpflug-based corneal tomography, mean image brightness (corneal densitometry) from the anterior 120 μm of the midcornea, and posterior 60 μm of the central 6 mm of the cornea, and HOAs were evaluated...
August 23, 2017: Cornea
https://www.readbyqxmd.com/read/28835759/associated-factors-of-bone-mineral-density-and-osteoporosis-in-elderly-males
#16
Behzad Heidari, Abdollah Muhammadi, Yahya Javadian, Ali Bijani, Reza Hosseini, Mansour Babaei
BACKGROUND: Low bone mineral density and osteoporosis is prevalent in elderly subjects. This study aimed to determine the associated factors of bone mineral density and osteoporosis in elderly males. METHODS: All participants of the Amirkola health and ageing project cohort aged 60 years and older entered the study. Bone mineral density at femoral neck and lumbar spine was assessed by the dual energy X-ray absorptiometry (DXA) method. Osteoporosis was diagnosed by the international society for clinical densitometry criteria and the association of bone mineral density and osteoporosis with several clinical, demographic and biochemical parameters...
January 2017: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28827962/high-performance-thin-layer-chromatography-densitometry-method-for-determination-of-rubraxanthone-in-the-stem-bark-extract-of-garcinia-cowa-roxb
#17
Dachriyanus Hamidi, Hilyatul Aulia, Meri Susanti
CONTEXT: Garcinia cowa is a medicinal plant widely grown in Southeast Asia and tropical countries. Various parts of this plant have been used in traditional folk medicine. The bark, latex, and root have been used as an antipyretic agent, while fruit and leaves have been used as an expectorant, for indigestion and improvement of blood circulation. AIMS: This study aims to determine the concentration of rubraxanthone found in ethyl acetate extract of the stem bark of G...
July 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/28817393/increased-expiratory-computed-tomography-density-reveals-possible-abnormalities-in-radiologically-preserved-lung-parenchyma-in-idiopathic-pulmonary-fibrosis
#18
Valentina Petroulia, Manuela Funke, Pascal Zumstein, Sabina Berezowska, Lukas Ebner, Thomas Geiser, Nenad Torbica, Johannes Heverhagen, Alexander Poellinger
OBJECTIVES: Idiopathic pulmonary fibrosis (IPF) is a progressive lethal chronic lung disease with unclear pathogenesis. Radiological hallmark is the pattern of usual interstitial pneumonia accentuated in peripheral and basal areas with otherwise preserved lung structure. One hypothesis is that alveolar collapse and consequent induration lead to fibrotic transformation of lung tissue. The aim of the study was to investigate normal-appearing tissue during expiration for signs of collapsibility and differences from other diseases or controls...
August 16, 2017: Investigative Radiology
https://www.readbyqxmd.com/read/28814844/comparison-between-parameters-of-muscle-performance-and-inflammatory-biomarkers-of-non-sarcopenic-and-sarcopenic-elderly-women
#19
Lygia Paccini Lustosa, Patrícia Parreira Batista, Daniele Sirineu Pereira, Leani Souza Máximo Pereira, Aline Scianni, Giane Amorim Ribeiro-Samora
BACKGROUND: Sarcopenia is a multifactorial geriatric syndrome with complex interrelationships. Increased plasma levels of inflammatory mediators increase the catabolic stimuli of the musculature, thereby causing a decrease in mass and muscular function. OBJECTIVE: The objective of this study was to compare the performance of the knee extensors test (by isokinetic dynamometer) and plasma levels of interleukin-6 (IL-6) and soluble receptors of tumor necrosis factor alpha (sTNFR1) between sarcopenics and non-sarcopenics community-dwelling elderly women residents of Brazil...
2017: Clinical Interventions in Aging
https://www.readbyqxmd.com/read/28810118/capacitive-detection-of-low-enthalpy-higher-order-phase-transitions-in-synthetic-and-natural-composition-lipid-membranes
#20
Graham Jeffrey Taylor, Frederick A Heberle, Jason Sharp Seinfeld, John Katsaras, C Patrick Collier, Stephen Andrew Sarles
In-plane lipid organization and phase separation in natural membranes plays a key role in regulating many cellular processes. Highly cooperative, first-order phase transitions in model membranes consisting of few lipid components are well understood and readily detectable via calorimetry, densitometry, and fluorescence. However, far less is known about natural membranes containing numerous lipid species and high concentrations of cholesterol, for which thermotropic transitions are undetectable by the above-mentioned techniques...
August 15, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
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