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densitometry

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https://www.readbyqxmd.com/read/28643464/adiponectin-isoform-patterns-in-ethnic-specific-adipoq-mutation-carriers-the-iras-family-study
#1
Keri L Tabb, Chuan Gao, Pamela J Hicks, Gregory A Hawkins, Jerome I Rotter, Yii-Der Ida Chen, Xiuqing Guo, Jill M Norris, Carlos Lorenzo, Barry I Freedman, Donald W Bowden, Nicholette D Palmer
OBJECTIVE: Adiponectin is found in human serum in three groups of multimers (high molecular weight [HMW], medium molecular weight [MMW], and low molecular weight [LMW]). Two ethnic-specific variants in ADIPOQ, G45R (Hispanic-Americans) and R55C (African-Americans), were previously reported. Although carriers of both variants had mean adiponectin levels ≤ 20% of those of noncarriers, they were not clinically different from noncarriers. To compare carriers of both variants and noncarriers, relative quantification of adiponectin isoforms to total adiponectin was performed on serum samples...
June 23, 2017: Obesity
https://www.readbyqxmd.com/read/28634907/incidence-and-predictors-of-repeat-bone-mineral-densitometry-a-longitudinal-cohort-study
#2
Emily C White VanGompel, Peter Franks, John A Robbins, Joshua J Fenton
BACKGROUND: Existing guidelines for repeat screening and treatment monitoring intervals regarding the use of dual-energy x-ray absorptiometry (DXA) scans are conflicting or lacking. The Choosing Wisely campaign recommends against repeating DXA scans within 2 years of initial screening. It is unclear how frequently physicians order repeat scans and what clinical factors contribute to their use. OBJECTIVE: To estimate cumulative incidence and predictors of repeat DXA for screening or treatment monitoring in a regional health system...
June 20, 2017: Journal of General Internal Medicine
https://www.readbyqxmd.com/read/28630785/assessment-of-corneal-densitometry-in-rheumatoid-arthritis-patients
#3
Mustafa Alpaslan Anayol, Başak Bostancı, Mehmet Ali Şekeroğlu, Mert Şimşek, Süleyman Günaydın, Pelin Yılmazbaş
OBJECTIVES: To evaluate corneal densitometry and anterior segment parameters of rheumatoid arthritis (RA) patients and compare these results with those of age-matched healthy control subjects. MATERIALS AND METHODS: Anterior segment parameters and corneal densitometry of patients with RA and healthy control subjects were assessed by Scheimpflug corneal topography. For densitometry analysis, the 12-mm diameter area of the cornea was subdivided into four concentric radial zones and anterior, central, and posterior layers based on corneal depth...
June 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/28630072/pseudomonas-quinolone-signal-pqs-induces-oxidative-stress-and-inhibits-heme-oxygenase-1-expression-in-lung-epithelial-cells
#4
Maher Y Abdalla, Traci Hoke, Javier Seravalli, Barbara L Switzer, Melissa Bavitz, Jill D Fliege, Peter J Murphy, Bradley E Britigan
Pseudomonas aeruginosa (PA) causes lung infection in patients with cystic fibrosis (CF). Pseudomonas Quinolone Signal (PQS) is a secreted PA virulence factor that contributes to the pathogenicity of PA. We were able to detect PQS in sputum samples from CF patients infected with PA, but not in samples from uninfected patients. We then tested the hypothesis that PQS induces oxidative stress in host cells by determining the ability of PQS to induce production of reactive oxygen species (ROS) in lung epithelial cells (A549 and primary normal human bronchial epithelial (NHBE)) and macrophages (J774A...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28629885/-prevalence-of-osteoporosis-estimation-of-probability-of-fracture-and-bone-metabolism-study-in-patients-with-newly-diagnosed-prostate-cancer-in-the-health-area-of-lugo
#5
Jonatan Miguel-Carrera, Carlos García-Porrua, Francisco Javier de Toro Santos, Jose Antonio Picallo-Sánchez
OBJECTIVE: To study the prevalence of osteoporosis and fracture probability in patients diagnosed with prostate cancer. DESIGN: Observational descriptive transversal study. SITE: Study performed from Primary Care of Lugo in collaboration with Rheumatology and Urology Services of our referral hospital. PARTICIPANTS: Patients diagnosed with prostate cancer without bone metastatic disease from January to December 2012. MAIN MEASUREMENTS: Epidemiologic, clinical, laboratory and densitometric variables involved in osteoporosis were collected...
June 16, 2017: Atencion Primaria
https://www.readbyqxmd.com/read/28627088/astrogliosis-and-impaired-aquaporin-4-and-dystrophin-systems-in-idiopathic-normal-pressure-hydrocephalus
#6
Per Kristian Eide, Hans-Arne Hansson
AIMS: Idiopathic normal pressure hydrocephalus (iNPH) is one subtype of dementia that may improve following drainage of cerebrospinal fluid (CSF). This prospective observational study explored whether expression of the water channel aquaporin-4 (AQP4) and the anchoring molecule dystrophin 71 (Dp71) are altered at astrocytic perivascular endfeet and in adjacent neuropil of iNPH patient. Observations were related to measurements of pulsatile and static intracranial pressure (ICP). METHODS: The study included iNPH patients undergoing overnight monitoring of the pulsatile/static ICP, in whom a biopsy was taken from the frontal cerebral cortex during placement of the ICP sensor...
June 19, 2017: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/28625918/body-composition-by-dxa
#7
John A Shepherd, Bennett K Ng, Markus J Sommer, Steven B Heymsfield
Body composition measurements from DXA have been available since DXA technology was developed 30years ago, but are historically underutilized. Recently, there have been rapid developments in body composition assessment including the analysis and publication of representative data for the US, official usage guidance from the International Society for Clinical Densitometry, and development of regional body composition measures with clinical utility. DXA body composition is much more than whole body percent fat...
June 16, 2017: Bone
https://www.readbyqxmd.com/read/28622316/corneal-densitometry-after-photorefractive-keratectomy-laser-assisted-in-situ-keratomileusis-and-small-incision-lenticule-extraction
#8
F Poyales, N Garzón, J Mendicute, I Illarramendi, P Caro, O Jáñez, F Argüeso, A López
PurposeThe aim of this study was to gain greater insight into the corneal densitometry changes occurring as a result of refractive surgery and to compare these changes across three widely used surgical techniques, namely, photorefractive keratectomy (PRK), laser-assisted in situ keratomileusis with a femtosecond laser (LASIK-FS), or ReLEx small-incision lenticule extraction (ReLEx SMILE).Patients and methodsThree hundred and thirty-six patients (184 male and 152 female patients) participated in this study. They were split into three groups according to the refractive surgery technique they had undergone: LASIK-FS (74 patients), PRK (153 patients), and ReLEx SMILE (109 patients)...
June 16, 2017: Eye
https://www.readbyqxmd.com/read/28621741/iron-deficiency-anemia-as-a-risk-factor-for-osteoporosis-in-taiwan-a-nationwide-population-based-study
#9
Mei-Lien Pan, Li-Ru Chen, Hsiao-Mei Tsao, Kuo-Hu Chen
The cause-effect relationship between iron deficiency anemia (IDA) and osteoporosis has not been established in the general population. Thus, the current longitudinal study determined the role of IDA as a risk factor for osteoporosis by analyzing a large nationwide population-based sample. In a sample of 1,000,000 randomly sampled individuals from the 1998-2012. Taiwan National Health Insurance Research Database, patients with IDA (case group (n = 35,751)) and individuals without IDA (control group (n = 178,755)) were compared...
June 16, 2017: Nutrients
https://www.readbyqxmd.com/read/28616759/is-dual-semitendinosus-allograft-stronger-than-turndown-for-achilles-tendon-reconstruction-an-in-vitro-analysis
#10
Michael C Aynardi, Lara C Atwater, Roshan Melvani, Brent G Parks, Adrian G Paez, Stuart D Miller
BACKGROUND: Large Achilles tendon defects pose a treatment challenge. The standard treatment with a turndown flap requires a large extensile incision, puts the sural nerve at risk, and demands slow, careful rehabilitation. Dual allograft semitendinosus reconstruction is a new clinical alternative that has the theoretical advantages of a smaller incision, less dissection, and a stronger construct that may allow for faster rehabilitation. QUESTIONS/PURPOSES: In a cadaver biomechanical model, we compared the dual allograft semitendinosus reconstruction with the myofascial turndown in terms of (1) mechanical strength and resistance to deformation and (2) failure mechanisms in reconstruction of large segmental Achilles defects...
June 14, 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/28612603/sortase-mediated-ligation-as-a-modular-approach-for-the-covalent-attachment-of-proteins-to-the-exterior-of-the-bacteriophage-p22-virus-like-particle
#11
Dustin P Patterson, Benjamin Schwarz, John Avera, Brian Western, Matthew Hicks, Paul Krugler, Matthew Terra, Masaki Uchida, Kimberly McCoy, Trevor Douglas
Virus-like particles are unique platforms well suited for the construction of nanomaterials with broad range applications. The research presented here describes the development of a modular approach for the covalent attachment of protein domains to the exterior of the versatile bacteriophage P22 virus-like particle (VLP) via a sortase-mediated ligation strategy. The bacteriophage P22 coat protein was genetically engineered to incorporate an LPETG amino acid sequence on the C-terminus, providing the peptide recognition sequence utilized by the sortase enzyme to catalyze peptide bond formation between the LPETG-tagged protein and a protein containing a poly-glycine sequence on the N-terminus...
June 14, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28612553/-obesity-and-bone-mineral-density-in-menopausal-women
#12
Kang An, Ken Qin, She-Yu Li, Han-Wei Yang, Qin-Qin Wu, Yan Huang, Chuan Yu, Zhen-Mei An, Shuang-Qing Li
OBJECTIVES: To determine the association between obesity and bone mineral density in menopausal women. METHODS: We recruited menopausal women aged 50 years and older who undertook health examinations in West China Hospital of Sichuan University in this study. Bone mineral density of the participants was measured by MetriScan Bone Densitometry. The participants were categorized into three groups according to the T value: normal density, osteopenia and osteoporosis...
January 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28612552/-obesity-and-osteoporosis-in-men-aged-above-50
#13
Ken Qin, Min He, Xiao-Tao Cao, Han-Wei Yang, Ying Yang, You-Juan Wang, Chuan Yu, Zhen-Mei An, She-Yu Li
OBJECTIVES: To investigate the association between obesity and osteoporosis in men aged above 50 in Chengdu. METHODS: Male participants aged above 50 were recruited from those who visited West China Hospital of Sichuan University for health examinations. Bone mineral density was measured by MetriScan Bone Densitometry. The participants were divided into three groups according to T values: normal, osteopenia and osteoporosis. RESULTS: About 5...
January 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28611211/metabolic-and-neuroprotective-effects-of-dapagliflozin-and-liraglutide-in-diabetic-mice
#14
Paul Millar, Nupur Pathak, Vadivel Parthsarathy, Tony Bjourson, Maurice O'Kane, Varun Pathak, R Charlotte Moffett, Peter R Flatt, Victor A Gault
This study assessed the metabolic and neuroprotective actions of the sodium glucose co-transporter-2 inhibitor dapagliflozin in combination with the GLP-1 agonist liraglutide in dietary-induced diabetic mice. Mice administered low-dose streptozotocin (STZ) on a high fat diet received dapagliflozin, liraglutide, dapagliflozin-plus-liraglutide (DAPA-Lira) or vehicle once-daily over 28 days. Energy intake, body weight, glucose and insulin concentrations were measured at regular intervals. Glucose tolerance, insulin sensitivity, hormone and biochemical analysis, dual-energy x-ray absorptiometry densitometry, novel object recognition, islet and brain histology were examined...
June 13, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28608217/multiplex-cathepsin-zymography-to-detect-amounts-of-active-cathepsins-k-l-s-and-v
#15
Manu O Platt
Cysteine cathepsins are powerful proteases that can degrade other proteins, among which are the extracellular matrix proteins collagen and elastin. Multiplex cathepsin zymography is an assay that can quantify the amount of active cathepsins in a cell or tissue preparation. This method works for measuring the amounts of active cathepsins K, L, S, and V in a cell or tissue preparation without requiring the use of antibodies for specific identification which tremendously reduces cost. This chapter will demonstrate the utility and interpretation of this method with mammalian cells and tissue to quantify amounts of active cathepsins K, L, S, and V without complicating signals of the procathepsin...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28608208/two-dimensional-zymography-of-proteases-from-steatotic-duck-liver
#16
Jeff Wilkesman, María Fernanda Padrón, Liliana Kurz, Hervé Rémignon
Protease activity present in liver cells with steatosis can be electrophoretically characterized. Zymographic techniques allow semi-quantitative results, successfully detecting cathepsin and metalloprotease activity using polyacrylamide gels copolymerized with gelatin and quantified by densitometry. By using specific inhibitors, the identity of the proteases can be confirmed. 2D zymography allows the determination of both M r. and pI of the metalloprotease and cathepsin activity present in the homogenates. The analysis of liver proteases activities in force fed ducks may elucidate the mechanisms behind steatosis development...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28606890/how-to-plan-an-overlap-free-projection-on-cta-or-fluoroscopy-to-facilitate-quantitative-analysis
#17
Justin Teng, Vincent Nijenhuis, Martin Swaans, Gerald Yong, Carl Schultz
AIMS: Assessment of para-valvular aortic regurgitation remains challenging. Quantitative video-densitometry is an effective technique however overlap of the aorta and left ventricle (LV) limits feasibility. We aimed to develop and validate a method to determine an 'Overlap Free Projection' (OFP). METHODS AND RESULTS: 65 computed tomography scans of patients referred for transcatheter aortic valve implantation were analysed using 3D reconstruction to evaluate the OFP...
June 13, 2017: EuroIntervention
https://www.readbyqxmd.com/read/28599895/variations-in-resting-energy-expenditure-impact-on-gestational-weight%C3%A2-gain
#18
Ek Berggren, P O'Tierney-Ginn, S Lewis, L Presley, S Hauguel De-Mouzon, P M Catalano
BACKGROUND: There are significant variations in gestational weight gain, with many women gaining in excess of the Institute of Medicine guidelines. Unfortunately, efforts to improve appropriate gestational weight gain have had only limited success. To date, interventions have focused primarily on decreasing energy intake and/or increasing physical activity. Maternal resting energy expenditure, which comprises ∼60% of total energy expenditure compared with the ∼20% that comes from physical activity, may be an important consideration in understanding variations in gestational weight gain...
June 17, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28595648/investigation-and-management-of-osteoporosis-in-aged-trauma-patients-a-treatment-algorithm-adapted-to-the-german-guidelines-for-osteoporosis
#19
Carl Neuerburg, Lena Mittlmeier, Ralf Schmidmaier, Christian Kammerlander, Wolfgang Böcker, Wolf Mutschler, Ulla Stumpf
BACKGROUND: Osteoporosis-associated fractures are of increasing importance in trauma surgery. Systematic diagnostics and treatment of osteoporosis during a hospital stay, however, remain inadequate. Therefore, a specific algorithm for diagnosing and treating osteoporosis in trauma surgery patients was developed based on the DVO (German Osteology Society) guideline for osteoporosis from 2014. METHODS: In a first step, the individuals' age and risk profile for osteoporosis is identified considering specific fractures indicating osteoporosis and risk factors assessed by a specific questionnaire...
June 8, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28595313/the-genome-sizes-of-ostracod-crustaceans-correlate-with-body-size-and-evolutionary-history-but-not-environment
#20
Nicholas W Jeffery, Emily A Ellis, Todd H Oakley, T Ryan Gregory
Within animals a positive correlation between genome size and body size has been detected in several taxa but not in others, such that it remains unknown how pervasive this pattern may be. Here we provide another example of a positive relationship in a group of crustaceans whose genome sizes have not previously been investigated. We analyze genome size estimates for 46 species across the two most diverse orders of Class Ostracoda, commonly known as seed shrimps, including 29 new estimates made using Feulgen image analysis densitometry and flow cytometry...
June 8, 2017: Journal of Heredity
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