Read by QxMD icon Read


John K Fallon, Nicole Houvig, Catherine L Booth-Genthe, Philip C Smith
Information is needed on the expression of transporters in lung to inform drug development and therapeutic decisions. Much of the information currently available is from semiquantitative gene expression or immunometric densitometry studies reported in the literature. NanoLC-MS/MS (MRM mode) isotope dilution targeted quantitative proteomics was used here to quantify twelve selected transporters in fresh human lung membrane fraction samples and in the membrane fraction of selected immortalized human lung epithelial cell line samples...
February 22, 2018: Journal of Pharmaceutical and Biomedical Analysis
Mehdi Shajari, Emanuel Wanner, Vladimir Rusev, Shervin Mir Mohi Sefat, Wolfgang J Mayer, Thomas Kohnen, Siegfried Priglinger, Daniel Kook
PURPOSE: To compare corneal densitometry values after Femtosecond Laser-Assisted In Situ Keratomileusis (Fs-LASIK) and Small Incision Lenticule Extraction (SMILE) using Scheimpflug Imaging. METHODS: Our retrospective clinical trial included a total of 146 eyes where 57 eyes underwent Fs-LASIK and 89 eyes underwent SMILE. Examinations with a Pentacam Scheimpflug device (Oculus, Wetzlar, Germany) were performed before surgery, 1 week, 1 month, 3 months, 6 months, and 1 year after surgery...
March 14, 2018: Current Eye Research
Val A Fajardo, John S Mikhaeil, Cameron F Leveille, A Russell Tupling, Paul J LeBlanc
BACKGROUND: An increase in phosphatidylcholine:phosphatidylethanolamine (PC:PE) and a decrease in fatty acyl chain length, monounsaturated:polyunsaturated (MUFA:PUFA) fatty acyl ratio reduces SERCA activity in liposomes and in mouse models of obesity and muscular dystrophy. We have previously shown that maximal SERCA activity is significantly reduced in mechanically overloaded (OVL) plantaris, however, whether changes in PC:PE ratio or fatty acyl composition may contribute to the alterations in maximal SERCA activity remain unknown...
March 13, 2018: Lipids in Health and Disease
Gabriel Gallo-Oller, Raquel Ordoñez-Ciriza, Javier Dotor
Since its first description, Western blot has been widely used in molecular labs. It constitutes a multistep method that allows the detection and/or quantification of proteins from simple to complex protein mixtures. Western blot quantification method constitutes a critical step in order to obtain accurate and reproducible results. Due to the technical knowledge required for densitometry analysis together with the resources availability, standard office scanners are often used for the imaging acquisition of developed Western blot films...
March 6, 2018: Journal of Immunological Methods
Neil M Carleton, Guangjing Zhu, Mikhail Gorbounov, M Craig Miller, Kenneth J Pienta, Linda M S Resar, Robert W Veltri
BACKGROUND: There are few tissue-based biomarkers that can accurately predict prostate cancer (PCa) progression and aggressiveness. We sought to evaluate the clinical utility of prostate and breast overexpressed 1 (PBOV1) as a potential PCa biomarker. METHODS: Patient tumor samples were designated by Grade Groups using the 2014 Gleason grading system. Primary radical prostatectomy tumors were obtained from 48 patients and evaluated for PBOV1 levels using Western blot analysis in matched cancer and benign cancer-adjacent regions...
March 9, 2018: Prostate
N Segaud, I Legroux, M Hazzan, C Noel, B Cortet
INTRODUCTION: In renal transplant patients, bone loss may be related to the drugs patients are taking but also to their past history of chronic kidney disease. The purpose of this study was to assess changes in BMD 2 years after an initial assessment (performed 9 months post transplantation) and the factors associated with these changes. METHODS: This longitudinal study included patients who had undergone a renal transplantation between 2005 and 2011, and who were followed up at the Lille Regional University Hospital...
March 2, 2018: Osteoporosis International
Myung Hee Chung, Bo Mi Gil, Soon Seog Kwon, Hae-Il Park, Sun Wha Song, Na Young Jung, Won Jong Yoo
OBJECTIVES: To determine what computed tomographic (CT) dimensions can predict obstructive lung disease on routine chest CT scans by comparing morphological and densitometric CT findings with pulmonary function test (PFT) in normal subjects and patients with chronic obstructive pulmonary disease (COPD). MATERIALS AND METHODS: Consecutive patients (n = 646; 260 females and 386 males; mean age 54.9 years, ranged 20-90 years) who received chest CT scans with densitometry during a 3-month period were retrospectively analyzed in single center...
March 2018: European Journal of Radiology
Benedicte De Muynck, Mathias Leys, Joke Cuypers, Dirk Vanderschueren, Marion Delcroix, Catharina Belge
We report the case of a 50-year-old woman with anorexigen-induced pulmonary arterial hypertension treated with epoprostenol, who presented with Trousseau's sign, leading to the diagnosis of severe hypocalcemia for which substitution was started (initially orally, followed by intravenous substitution). After further analysis, we assume that epoprostenol-induced diarrhea caused malabsorption (as other reasons were excluded), leading to nutritional osteomalacia with secondary hyperparathyroidism. We discovered that even more severe hypocalcemia was induced by the treatment with the anti-osteoporotic drug denosumab, which was started after the diagnosis of osteoporosis on bone densitometry...
2018: Respiration; International Review of Thoracic Diseases
George J Francis, Amy E Cook, Donald W Morrish, K Ming Chan, Hernish J Acharya
Many neuromuscular diseases (NMD) result in muscle weakness, immobility and greater fracture risk. The objective of this study is to determine the fracture risk of adult patients at a multidisciplinary NMD clinic. Fracture risk was calculated using the Fracture Risk Assessment Tool, the presence of osteoporosis was quantified using bone densitometry and contributing co-morbidities were screened through serum markers. Of the 36 patients studied, 47% were found to be of moderate and high fracture risk. Two thirds of these patients had not been previously screened or treated for osteoporosis...
2018: Journal of Neuromuscular Diseases
Nayane R Pierote, Amanda F Braz, Susy L Barros, José M Moita Neto, José Miguel L Parente, Maria da Cruz M Silva, Mayara S Beserra, Nina Rosa M Soares, Dilina N Marreiro, Nadir do Noscimento Nogueira
OBJECTIVES: Crohn's disease (CD) is a condition that is characterized by chronic inflammation. The presence of multifactorial pathogenesis that results from inflammation is associated with low micronutrient consumption and glucocorticoid use, which may be related to bone health. This study aimed to evaluate the relationship between dietary mineral intake and glucocorticoid use in bone mineral density (BMD) in patients with CD. METHODS: A cross-sectional study of 62 patients with CD ages 20 y to 40 y measured their macro- and micronutrient intake with a 3-d food record...
November 27, 2017: Nutrition
M Alnawaiseh, N Eter
BACKGROUND: Corneal densitometry, based on the Scheimpflug principle, is a useful noninvasive tool for quantitative evaluation of the optical quality of the cornea. It enables us to evaluate corneal transparency in the different corneal layers and different annuli. In this article, we discuss the basis of application of corneal densitometry measurements and summarize findings in patients with keratoconus. METHODS: This article is based on a selective literature review and analysis of own data...
February 21, 2018: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
Angelo A Licata, Neil Binkley, Steven M Petak, Pauline M Camacho
OBJECTIVE: High-quality dual-energy X-ray absorptiometry (DXA) scans are necessary for accurate diagnosis of osteoporosis and monitoring of therapy; however, DXA scan reports may contain errors that cause confusion about diagnosis and treatment. This American Association of Clinical Endocrinologists/American College of Endocrinology consensus statement was generated to draw attention to many common technical problems affecting DXA report conclusions and provide guidance on how to address them to ensure that patients receive appropriate osteoporosis care...
February 2018: Endocrine Practice
Małgorzata Janik, Michał Stuss, Marta Michalska-Kasiczak, Anna Jegier, Ewa Sewerynek
Osteoporosis is a serious medical and socioeconomic problem of the 21st century. Mechanical load is a key regulator which controls bone formation and remodelling, with participation of osteocytes. Sclerostin is produced and released by mature osteocytes into bone surface, where it inhibits the conveyance of osteoblast proliferation and differentiation activating signals from mesenchymal cells, thus suppressing new bone formation. The goal of the study was an evaluation of the effects of a 12-week physical training programme on the levels of bone turnover markers [Sclerostin, Osteocalcin (OC), C-terminal telopeptide of type I collagen (β-CTX)] in blood serum of women with osteopenia...
February 21, 2018: Endokrynologia Polska
Mohammad Sadegh Ahmad Akhoundi, Sedigheh Sheikhzadeh, Amirhossein Mirhashemi, Elahe Ansari, Yasaman Kheirandish, Mozhgan Allaedini, Ahmadreza Dehpour
PURPOSE: The aim of the present study was to evaluate the effect of the carbamazepine and valproic acid on orthodontic tooth movement in male Wistar rats. METHODS: Evaluation of tooth movement after 21 days of drugs infusion was carried out by feeler gauge. Bone densitometry on lateral cephalograms was conducted on days 1 and 21. After dissection of the maxillae, histologic parameters were evaluated. RESULTS: Orthodontic tooth movement was accelerated in experimental groups rather than controls...
February 16, 2018: International Orthodontics
Jaya Chawla, Neena Sharma, Devendra Arora, Manisha Arora, Lovely Shukla
OBJECTIVE: Osteoporosis is a skeletal disorder characterized by diminished bone strength that increases the risk of fracture at instances of trivial trauma. Asians have a lower bone mass than the west. The present study was designed to add data from India on women above the age of 40 years with respect to low bone mineral density (BMD) and its associated high risk factors. MATERIALS AND METHODS: After a written informed consent, a detailed history was taken. Basal metabolic index was recorded, and biochemical and endocrine tests were done, followed by dual X ray absorptiometry scan...
February 2018: Taiwanese Journal of Obstetrics & Gynecology
R S Tranter, P T Lynch, J B Randazzo, J P A Lockhart, X Chen, C F Goldsmith
The dissociation of 2-methyl furan at high temperatures has been studied in a combined experimental and theoretical approach to elucidate the details of this multi-channel unimolecular reaction. Laser schlieren densitometry studies were performed in a diaphragmless shock tube over the range 1600 < T < 2300 K and three pressures 60, 120 and 240 Torr. The theoretical study identified many reaction paths, most of which are initiated by the formation of carbenes. Of these paths, five account for 99% consumption of 2MF, and three account for 95% consumption...
February 16, 2018: Physical Chemistry Chemical Physics: PCCP
Korine van Dijk, Jack S Parker, Lamis Baydoun, Abbas Ilyas, Isabel Dapena, Esther A Groeneveld-van Beek, Gerrit R J Melles
PURPOSE: The purpose of this study was to evaluate the 5-year clinical results of isolated Bowman layer (BL) transplantation in the treatment of advanced keratoconus. METHODS: In this prospective, single-center, interventional case series at a tertiary referral center, 20 eyes of 17 patients with advanced keratoconus underwent BL transplantation, i.e. an isolated Bowman layer graft was positioned into a manually dissected mid-stromal pocket. Scheimpflug-based corneal tomography measurements, best corrected spectacle and contact lens visual acuities (BSCVA and BCLVA), endothelial cell density, and complications were evaluated up to 5 years after surgery...
February 14, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
Diana Crossley, Mary Renton, Muhammad Khan, Emma V Low, Alice M Turner
Background: The aim of the study was to assess the relationship between computed tomography (CT) densitometry and routine clinical markers in patients with chronic obstructive pulmonary disease (COPD) and alpha-1 anti-trypsin deficiency (AATD). Methods: Multiple databases were searched using a combination of pertinent terms and those articles relating quantitatively measured CT densitometry to clinical outcomes. Studies that used visual scoring only were excluded, as were those measured in expiration only...
2018: International Journal of Chronic Obstructive Pulmonary Disease
Zhaleh Shariati-Sarabi, Hamid Etemad Rezaie, Nasrin Milani, Farnaz Etemad Rezaie, Ali Etemad Rezaie
Background: The menopausal transition called perimenopause, happens after the reproductive years, and is specified with irregular menstrual cycles, perimenopause symptoms and hormonal changes. Women going through peri menopausal period are vulnerable to bone loss . Osteoporosis is one of the most common debilitating metabolic bone diseases, especially in the women almost around 50 years. This study was intended to evaluate the prevalence of osteopenia/osteoporosis amongst asymptomatic individuals during the menopause transition period...
January 2018: Archives of Bone and Joint Surgery
L Zhang, Y Wang, T Cui, W Zhao, W B Cheng
Objective: To evaluate the changes of corneal transparency over the 1-year period after small incision lenticule extraction (SMILE) according to corneal Scheimpflug densitometry. Methods: Prospective cohort study. Fifty-five patients (100 eyes) with myopia (19 male and 36 female, aged 18 to 39 years with average of 24) who underwent SMILE and regular follow-up for at least 1 year at Tianjin Eye Hospital were enrolled. Examinations took place before SMILE and postoperatively at 1 day, 1 week, and 1, 3, 6, and 12 months...
January 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"