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https://www.readbyqxmd.com/read/29241051/determination-of-lipid-profiles-in-serum-of-obese-ponies-before-and-after-weight-reduction-by-using-multi-one-dimensional-thin-layer-chromatography
#1
Ratchada Auyyuenyong, Andrea Henze, Julia Ungru, Florian J Schweigert, Jens Raila, Ingrid Vervuert
Obesity is a key component of equine metabolic syndrome, which is highly associated with laminitis. Feed restriction and/or exercise are known to alleviate the detrimental effects of insulin resistance in obese ponies. However, little is known about changes in the serum lipid patterns due to weight reduction and its association with disease outcomes. Therefore, the lipid patterns in the serum of 14 mature ponies before and after a 14-week body weight reduction program (BWRP) were investigated by multi-one-dimensional thin-layer chromatography (MOD-TLC)...
November 21, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29234729/a-comprehensive-study-of-eco-friendly-natural-pigment-and-its-applications
#2
Ramendra Singh Parmar, Charu Singh
Actinomycetes, a large group of filamentous bacteria account for 70-80% of secondary metabolites available commercially. The present investigation was undertaken with an aim to identify and characterize pigment from actinomycetes. Actinomycetes were isolated from rhizosphere soil samples collected from different regions of Madhya Pradesh state. Out of 85 actinomycetes, only 5 actinomycetes showed pigment production and based on diffusible pigment production ability one actinomycete ARITM02 was selected. The extraction of pigment was done by solvent extraction method using methanol and purified by TLC and column chromatography...
March 2018: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/29234421/comparative-phytochemical-profiling-and-in-vitro-antioxidant-activity-of-extracts-from-raw-materials-tissue-cultured-plants-and-callus-of-oroxylum-indicum-l-vent
#3
Piyanuch Rojsanga, Somnuk Bunsupa, Adelheid H Brantner, Pongtip Sithisarn
Extracts from raw materials from different plant parts, tissue-cultured plants, and callus cultures of Oroxylum indicum were analyzed for in vitro antioxidant activities determined by DPPH radical scavenging assay and evaluated for phytochemical profiles by TLC and LC-MS methods. The results were analyzed by principal component analysis (PCA) to evaluate the similarity. Stalk, pedicel, flower, seed, and whole fruit and callus extracts promoted strong antioxidant activity with high total phenolic and total flavonoid contents...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29232416/berberine-from-argemone-mexicana-l-exhibits-a-broadspectrum-antibacterial-activity
#4
Nilesh V More, Kiran R Kharat, Arun S Kharat
The Argemone mexicana L, commonly found on desolate land in the Marathwada region of Maharashtra state, India, has been used for treating oral cavity infections. We sought to investigate the antimicrobial potential of A. mexicana L. In this study, cold aqueous and methanolic extracts were prepared from the A. mexicana L leaves. These extracts were tested for their antibacterial activities against selected bacterial isolates. The antibacterial activity and MICs were tested using the agar well diffusion method and broth dilution method, respectively...
December 13, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29232391/studies-of-the-structure-antioxidant-activity-relationships-and-antioxidant-activity-mechanism-of-iridoid-valepotriates-and-their-degradation-products
#5
Feifei Wang, Yumei Zhang, Shouhai Wu, Yi He, Zhong Dai, Shuangcheng Ma, Bin Liu
Oxidative stress has been associated with diverse diseases, including obesity, cancer and neurodegeneration. In fact, Valeriana jatamansi Jones (valerian) and its extracts possess strong antioxidant activities that extend their application in clinical practice to the treatment of these illnesses, even though the underlying mechanisms are not well understood. Iridoid valepotriate, a characteristic iridoid ester in valerian with poor chemical stability, possesses considerable antioxidant components. The original compounds and their degradation products have been found to exhibit strong antioxidant activities...
2017: PloS One
https://www.readbyqxmd.com/read/29226507/age-is-the-only-predictor-of-small-decrease-in-lung-function-in-children-with-sickle-cell-anemia
#6
Shaina M Willen, Robyn Cohen, Mark Rodeghier, Fenella Kirkham, Susan S Redline, Carol Rosen, Jane Kirkby, Michael R DeBaun
The longitudinal pattern of lung function in children with sickle cell anemia (SCA) has shown a decrease in FEV1 % predicted, a risk factor for death in adults with SCA, but predictors for this decline are poorly characterized. In a prospective longitudinal multi-center cohort of children with SCA, we tested the hypotheses that: 1) FEV1 % predicted declines over time; and 2) SCA-specific characteristics and therapy predict this decline. At three clinical centers, children with SCA (HbSS or HbSβ0 thalassemia), unselected for respiratory disease, were enrolled in the Sleep and Asthma Cohort (SAC) study...
December 11, 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/29223449/anti-hsv-2-activity-of-peganum-harmala-l-and-isolation-of-the-active-compound
#7
Roudaina Benzekri, Lamjed Bouslama, Adele Papetti, Majdi Hammami, Abderrazak Smaoui, Ferid Limam
Genital herpes is a sexually transmitted disease caused by herpes simplex virus type 2 (HSV-2). Nucleoside analogues such as acyclovir (ACV) are the usual therapy for treating HSV infection. However, the overuse of this drug has led to the emergence of resistant strains. Therefore, the search for new alternative or complementary molecules to overcome this obstacle is needed. In this objective, Peganum harmala was investigated for its HSV-2 activity. The organic extracts of the different plant organs were evaluated for their cytotoxicity on Vero cells by the MTT test and anti HSV-2 activity by plaque reduction assay...
December 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29221717/effect-of-metformin-combined-with-lifestyle-modification-versus-lifestyle-modification-alone-on-proinflammatory-oxidative-status-in-drug-na%C3%A3-ve-pre-diabetic-and-diabetic-patients-a-randomized-controlled-study
#8
Nailya Bulatova, Violet Kasabri, Amenah Qotineh, Taiba Al-Athami, Al-Motassem Yousef, Salah AbuRuz, Munther Momani, Aymen Zayed
BACKGROUND: Targeting biomarkers of oxidative-proinflammatory stress may result in improvement of modifiable metabolic syndrome, pre-diabetes and diabetes risk factors and subsequent risk reduction. METHODS: 64 newly diagnosed antihyperglycemic treatment-naïve prediabetic and type 2 diabetes mellitus (T2DM) patients were randomly assigned using block design to either metformin combined with therapeutic lifestyle changes (TLC) or TLC alone. Body mass index (BMI), waist circumference, blood pressure, fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), fasting lipid profile, plasma oxidative status and tumor necrosis factor (TNF)-α were measured at baseline, after 3 months and after 6 months from baseline...
November 23, 2017: Diabetes & Metabolic Syndrome
https://www.readbyqxmd.com/read/29216590/improved-bioautographic-assay-on-tlc-layers-for-qualitative-and-quantitative-estimation-of-xanthine-oxidase-inhibitors-and-superoxide-scavengers
#9
Yao Kong, Xiangkun Li, Na Zhang, Yu Miao, Haiyan Feng, Tao Wu, Zhihong Cheng
A new agar-free bioautographic assay for xanthine oxidase (XO) inhibitors and superoxide scavengers on TLC layers was developed and validated. Compared to the first version of TLC bioautographic agar overlay method, our bioautographic assay greatly improved the sensitivity and quantification ability. The limit of detection (LOD) of this assay was 0.017ng for allopurinol. Quantitative estimation of XO inhibitors and superoxide scavengers was achieved by densitometry scanning, expressed as allopurinol equivalents in millimoles on a per sample weight basis...
December 2, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29215578/evaluation-of-antibiotic-residues-in-raw-meat-using-different-analytical-methods
#10
Tsepo Ramatla, Lubanza Ngoma, Modupeade Adetunji, Mulunda Mwanza
Antibiotic residue in meat is a serious public health concern due to its harmful effects on consumer health. This study aimed at estimating the residue levels of four commonly used antibiotics in meat samples using three analytical methods (ELISA, TLC and HPLC). A total of 150 samples of raw meat from sales points were analysed for ciprofloxacin, streptomycin, tetracycline, and sulphanilamide residues. Overall, ELISA analysis showed that 56, 34, 18, and 25.3% of the samples tested positive for ciprofloxacin, streptomycin, sulphanilamide and tetracycline residues respectively while TLC and HPLC detected 21...
December 7, 2017: Antibiotics
https://www.readbyqxmd.com/read/29213351/spondias-purpurea-l-anacardiaceae-antioxidant-and-antiulcer-activities-of-the-leaf-hexane-extract
#11
Cynthia Layse Ferreira de Almeida, Samara Alves Brito, Temístocles Italo de Santana, Henrique Bandeira Alves Costa, Carlson Helder Reis de Carvalho Júnior, Márcia Vanusa da Silva, Lécio Leone de Almeida, Larissa Araújo Rolim, Vanda Lucia Dos Santos, Almir Gonçalves Wanderley, Teresinha Gonçalves da Silva
Spondias purpurea is used in folk medicine to treat diarrhea and diuresis. The objective of this study was to evaluate the phytochemical profile and antioxidant and antiulcer activities of the hexane extract of the leaves of S. purpurea (SpHE). Phytochemical profile was evaluated via thin layer chromatography (TLC) and HPLC. SpHE was screened for antioxidant activities using DPPH, ABTS, FRAP, and phosphomolybdenum assays. To determine its antiulcer properties, animals were pretreated with injured control, lansoprazole, ranitidine, carbenoxolone, or SpHE (12...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29208759/the-role-of-multidetector-computed-tomography-and-the-forced-oscillation-technique-in-assessing-lung-damage-in-adults-with-cystic-fibrosis
#12
Letícia S Lacerda, Agnaldo J Lopes, Alysson R S Carvalho, Alan R M Guimarães, Mônica C Firmida, Marcos C S Castro, Roberto Mogami, Pedro L Melo
BACKGROUND: With increased survival rates and the consequent emergence of an adult population with cystic fibrosis (CF), developing novel tools for periodic evaluations of these patients has become a new challenge. Thus, we sought to determine the contribution of lung-volume quantification using multidetector computed tomography (CT) in adults with CF and to investigate the association between structural changes and functional abnormalities. METHODS: This was a cross-sectional study in which 21 adults with CF and 22 control subjects underwent lung-volume quantification using multidetector CT...
December 5, 2017: Respiratory Care
https://www.readbyqxmd.com/read/29207022/role-of-the-c-jun-n-terminal-kinase-signaling-pathway-in-the-activation-of-trypsinogen-in-rat-pancreatic-acinar-cells
#13
Zhengpeng Yang, Weiguang Yang, Ming Lu, Zhituo Li, Xin Qiao, Bei Sun, Weihui Zhang, Dongbo Xue
Bile acid causes trypsinogen activation in pancreatic acinar cells through a complex process. Additional research is required to further elucidate which signaling pathways affect trypsinogen activation when activated. the changes in the whole‑genome expression profile of AR42J cells under the effect of taurolithocholic acid 3‑sulfate (TLC‑S) were investigated. Furthermore, gene groups that may play a regulatory role were analyzed using the modular approach of biological networks. The aim of the present study was to improve our understanding of the changes in TLC‑S‑stimulated AR42J cells through a genetic functional modular analysis...
November 17, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29205724/growth-modeling-of-the-green-microalga-scenedesmus-obliquus-in-a-hybrid-photobioreactor-as-a-practical-tool-to-understand-both-physical-and-biochemical-phenomena-in-play-during-algae-cultivation
#14
Deise Parolo Tramontin, Pablo Diego Gressler, Leonardo Rubi Rörig, Roberto Bianchini Derner, Jurandir Pereira Filho, Claudemir Marcos Radetski, Marintho Bastos Quadri
In recent years, numerous studies have justified the use of microalgae as a sustainable alternative for the generation of different types of fuels, food supplementation and cosmetics, as well as bioremediation processes. To improve the cost/benefit ratio of microalgae mass production, many culture systems have been built and upgraded. Mathematical modeling the growth of different species in different systems has become an efficient and practical tool to understand both physical and biochemical phenomena in play during algae cultivation...
December 2, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29202741/the-influence-of-osmunda-regalis-root-extract-on-head-and-neck-cancer-cell-proliferation-invasion-and-gene-expression
#15
Marianne Schmidt, Josef Skaf, Georgiana Gavril, Christine Polednik, Jeanette Roller, Michael Kessler, Ulrike Holzgrabe
BACKGROUND: According to only a handful of historical sources, Osmunda regalis, the royal fern, has been used already in the middle age as an anti-cancer remedy. To examine this ancient cancer cure, an ethanolic extract of the roots was prepared and analysed in vitro on its effectiveness against head and neck cancer cell lines. METHODS: Proliferation inhibition was measured with the MTT assay. Invasion inhibition was tested in a spheroid-based 3-D migration assay on different extracellular matrix surfaces...
December 4, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29202361/a-novel-adenine-releasing-assay-for-ribosome-inactivating-proteins
#16
Alexander Weng
Ribosome-inactivating proteins (RIPs) are toxic enzymes that are mostly biosynthesized by plants. RIPs are N-glycosidases that cleave an essential adenine molecule from the 28S rRNA. This is followed by the irreversible inhibition of protein synthesis leading to cell death. By fusing RIPs to cancer cell specific targeting ligands RIPs have been utilized for targeted anti-tumor therapy. The anti-tumoral efficiency of such conjugates depends significantly on the N-glycosidase activity of the RIP domain. Different methods have been developed in order to determine the N-glycosidase activity of RIPs and RIP domain containing anti-tumor toxins...
November 28, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29200731/brazilian-cerrado-qualea-grandiflora-mart-leaves-exhibit-antiplasmodial-and-trypanocidal-activities-in-vitro
#17
Thuany de Moura Cordeiro, Fabian Borghetti, Sarah C Caldas Oliveira, Izabela Marques Dourado Bastos, Jaime Martins de Santana, Philippe Grellier, Sébastien Charneau
Background: The rapid spread of drug-resistant strains of protozoan parasites required the urgent need for new effective drugs. Natural products offer a variety of chemical structures, which make them a valuable source of lead compounds for the development of such new drugs. Cerrado is the second largest biome in Brazil and has the richest flora of all the world savannahs. We selected Qualea grandiflora, a plant species known for its proprieties in folk medicine and its antibacterial activity...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29200724/bioassay-guided-isolation-of-neuroprotective-fatty-acids-from-nigella-sativa-against-1-methyl-4-phenylpyridinium-induced-neurotoxicity
#18
Leila Hosseinzadeh, Hoda Monaghash, Farahnaz Ahmadi, Nastaran Ghiasvand, Yalda Shokoohinia
Objective: Parkinson's disease, a slowly progressive neurological disease, is associated with degeneration of the basal ganglia of the brain and a deficiency of the neurotransmitter dopamine. The main aspects of researches are the protection of normal neurons against degeneration. Fatty acids (FAs), the key structural elements of dietary lipids, are carboxylic straight chains and notable parameters in nutritional and industrial usefulness of a plant. Materials and Methods: Black cumin, a popular anti-inflammatory and antioxidant food seasoning, contains nonpolar constituents such as FAs which were extracted using hexane...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29200717/in-vitro-and-in-vivo-activity-of-myrsine-africana-on-elastase-inhibition-and-anti-wrinkle-activity
#19
Namrita Lall, Navneet Kishore, Bianca Fibrich, Isa Anina Lambrechts
Background: Myrsine africana (MA) is a plant traditionally used in South Africa to treat various diseases. Objective: The ethanolic extract of MA, was used for in vitro and in vivo studies to determine its elastase inhibitory activity. Materials and Methods: MA and its isolated compound, myrsinoside B, were tested in vitro for their elastase inhibitory activity. The MA extract was also evaluated for mutagenicity using two strains of Salmonella typhimurium (TA 98 and TA 100), microbial count, metal analysis, and stability...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29198414/profiling-and-determination-of-phenolic-compounds-in-indian-marketed-hepatoprotective-polyherbal-formulations-and-their-comparative-evaluation
#20
Sathis Kumar Dinakaran, Balla Sujiya, Harani Avasarala
BACKGROUND: Nowadays, plants have been considered as powerful agents for treatment of disorders due to their traditional use. Plants have a special role in the treatment of various diseases in Ayurveda. Liver disorders with their devastating outcomes have been discussed in Ayurveda as well. OBJECTIVES: In the present study, polyherbal products (L52 and L38) were retrieved from Ayurveda and its pharmacognostic standardization was performed. MATERIALS AND METHODS: Quality control test for the Ayurveda tablets were performed as per Indian Pharmacopoeia...
November 29, 2017: Journal of Ayurveda and Integrative Medicine
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