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https://www.readbyqxmd.com/read/28088311/prevalence-of-preoperative-penile-abnormalities-among-voluntary-male-medical-circumcision-patients-in-swaziland
#1
Anthony R Oddo, Elizabeth Ruedrich, Christopher Zust, Lindsey Marugg, Echo VanderWal, Harry VanderWal, Rebekah Sartori, Ronald Markert, Mary C McCarthy
BACKGROUND: Circumcision has been found to be an effective strategy for lowering the transmission of HIV in Africa. The Luke Commission, a mobile hospital outreach program, has used this information to decrease the rate of HIV in Swaziland by performing voluntary male medical circumcisions throughout the country. During many of these circumcisions, genital medical conditions and penile abnormalities are simultaneously discovered and corrected. PURPOSE: The goal of our study was to evaluate the prevalence of penile abnormalities discovered and treated during voluntary male medical circumcisions performed by The Luke Commission (TLC) throughout rural Swaziland...
January 3, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28079317/human-umbilical-cord-blood-cd34-positive-cells-as-predictors-of-the-incidence-and-short-term-outcome-of-neonatal-hypoxic-ischemic-encephalopathy-a-pilot-study
#2
Sahar M A Hassanein, Mohamed Hassan Nasr Eldin, Hanaa A Amer, Adel E Abdelhamid, Moustafa El Houssinie, Abir Ibrahim
BACKGROUND AND PURPOSE: Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of neurological handicap in developing countries. Human umbilical cord blood (hUCB) CD34-positive (CD34⁺) stem cells exhibit the potential for neural repair. We tested the hypothesis that hUCB CD34⁺ stem cells and other cell types [leukocytes and nucleated red blood cells (NRBCs)] that are up-regulated during the acute stage of perinatal asphyxia (PA) could play a role in the early prediction of the occurrence, severity, and mortality of HIE...
January 2017: Journal of Clinical Neurology
https://www.readbyqxmd.com/read/28077963/quality-control-of-lycium-chinense-and-lycium-barbarum-cortex-digupi-by-hplc-using-kukoamines-as-markers
#3
Yuan-Yuan Li, Rui Di, Wing-Leung Hsu, Ye-Qing Huang, Hon-Yeung Cheung
BACKGROUND: Lycii Cortex (LyC), composed of Lycium chinense and Lycium barbarum cortex and having the Chinese name Digupi, is used to treat chronic diseases like cough, hypertension, and diabetes in Eastern Asia. However, chromatographic methods, such as TLC and HPLC, to determine the phytochemical composition of LyC have not been included in any official compendiums. This study aims to establish a validated HPLC method for quality control of LyC. METHODS: Kukoamines A and B (KA and KB, respectively) were selected as markers for the HPLC method...
2017: Chinese Medicine
https://www.readbyqxmd.com/read/28077140/indacaterol-and-glycopyrronium-versus-indacaterol-on-body-plethysmography-measurements-in-copd-a-randomised-controlled-study
#4
Joerg Salomon, Daiana Stolz, Guido Domenighetti, Jean-Georges Frey, Alexander J Turk, Andrea Azzola, Thomas Sigrist, Jean-William Fitting, Ulrich Schmidt, Thomas Geiser, Corinne Wild, Konstantinos Kostikas, Andreas Clemens, Martin Brutsche
BACKGROUND: Dual bronchodilator therapy is recommended for symptomatic patients with chronic obstructive pulmonary disease (COPD). There are limited data on effects of a combination of two long-acting bronchodilators on lung function including body plethysmography. METHODS: This multicentre, randomised, double-blind, single-dose, cross-over, placebo-controlled study evaluated efficacy and safety of the free combination of indacaterol maleate (IND) and glycopyrronium bromide (GLY) versus IND alone on spirometric and body plethysmography parameters, including inspiratory capacity (IC), forced expiratory volume in 1 s (FEV1), forced vital capacity (FVC), total lung capacity (TLC) and airway resistance (Raw) in moderate-to-severe COPD patients...
January 11, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28075116/metal-organic-frameworks-for-thin-layer-chromatographic-applications
#5
Claudia Schenk, Christel Kutzscher, Franziska Drache, Stella Helten, Irena Senkovska, Stefan Kaskel
Preparation of thin-layer chromatographic (TLC) plates based on metal-organic frameworks (MOFs) as porous stationary phases is described. DUT-67 (DUT = Dresden University of Technology), a zirconium based MOF, was used in combination with a fluorescent indicator as stationary phase for analyzing a small selection of a wide spectrum of relevant analytes. The successful separation of benzaldehyde from trans-cinnamaldehyde and 4-aminophenol from 2-aminotoluene is reported as a model system using optimized eluent mixtures containing acetic acid...
January 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28071525/additive-toxic-effect-of-deltamethrin-and-cadmium-on-hepatic-hematological-and-immunological-parameters-in-mice
#6
Hasibur Rehman, Al Thbiani Aziz, Shalini Saggu, Adam L VanWert, Nahla Zidan, Sundeep Saggu
Exposure to natural and man-made environmental toxins concurrently can pose a greater threat to multiple organs. In the present work, we investigated interactions between deltamethrin (DM) and cadmium (Cd), whose mechanisms of action in humans are poorly understood. Albino mice were randomly divided into four groups, each containing six mice: saline as control, DM-treated, cadmium chloride (CdCl2)-treated, and CdCl2 plus DM treated. After 2 weeks of treatment biochemical and hematological effects, total leukocyte count (TLC), differential leukocyte count, humoral-mediated immune responses, and histopathological studies were conducted...
January 1, 2017: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/28068996/non-linear-parameters-of-specific-resistance-loops-to-characterise-obstructive-airways-diseases
#7
Marko Topalovic, Vasileios Exadaktylos, Thierry Troosters, Geert Celis, Jean-Marie Aerts, Wim Janssens
BACKGROUND: Specific resistance loops appear in different shapes influenced by different resistive properties of the airways, yet their descriptive ability is compressed to a single parameter - its slope. We aimed to develop new parameters reflecting the various shapes of the loop and to explore their potential in the characterisation of obstructive airways diseases. METHODS: Our study included 134 subjects: Healthy controls (N = 22), Asthma with non-obstructive lung function (N = 22) and COPD of all disease stages (N = 90)...
January 9, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28066105/antifertility-activity-of-thevetia-peruviana-pers-k-schum-leaf-in-female-sprague-dawley-rat
#8
Jhuma Samanta, Snehendu Bhattacharya, Avtar C Rana
OBJECTIVES: Thevetia peruviana (Pers.) K. Schum. (Apocynaceae) is known to possess cardioactive glycoside such as thevetin A, thevetin B, neriifolin, peruvoside, thevetoxin, and ruvoside. Traditionally, T. peruviana leaves are used as abortifacient. The aim of the present study is to evaluate antifertility potential of T. peruviana leaves. SUBJECTS AND METHODS: Cardiac glycoside freed leaves of T. peruviana were extracted with methanol using maceration method. The dried cardiac glycoside-free methanolic extract of T...
November 2016: Indian Journal of Pharmacology
https://www.readbyqxmd.com/read/28053522/do-nanoparticles-provide-a-new-opportunity-for-diagnosis-of-distal-airspace-disease
#9
Jakob Löndahl, Jonas Kf Jakobsson, David M Broday, H Laura Aaltonen, Per Wollmer
There is a need for efficient techniques to assess abnormalities in the peripheral regions of the lungs, for example, for diagnosis of pulmonary emphysema. Considerable scientific efforts have been directed toward measuring lung morphology by studying recovery of inhaled micron-sized aerosol particles (0.4-1.5 µm). In contrast, it is suggested that the recovery of inhaled airborne nanoparticles may be more useful for diagnosis. The objective of this work is to provide a theoretical background for the use of nanoparticles in measuring lung morphology and to assess their applicability based on a review of the literature...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28050681/detection-of-chlorophylls-in-spores-of-seven-ferns
#10
Mei-Hwei Tseng, Kuei-Huei Lin, Yi-Jia Huang, Ya-Lan Chang, Sheng-Cih Huang, Li-Yaung Kuo, Yao-Moan Huang
Fern spores were traditionally classified into chlorophyllous (green) and nonchlorophyllous (nongreen) types based on the color visible to the naked eye. Recently, a third type, "cryptochlorophyllous spores", is recognized, and these spores are nongreen under white light but contain chlorophylls. Epifluorescence microscopy was previously used to detect chlorophylls in cryptochlorophyllous spores. In addition to epifluorescence microscopy, current study performed some other approaches, including spore-squash epifluorescence, absorption spectra, laser-induced fluorescence emission spectra, thin layer chromatography (TLC), and ultra-high performance liquid chromatography with ultraviolet and mass spectrometric detection (UHPLC-UV-MS) in order to detect chlorophylls of spores of seven ferns (Sphaeropteris lepifera, Ceratopteris thalictroides, Leptochilus wrightii, Leptochilus pothifolius, Lepidomicrosorum buergerianum, Osmunda banksiifolia, and Platycerium grande)...
January 3, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28050475/role-of-hrct-chest-in-post-stem-cell-transplant-recipients-suspected-of-pulmonary-complications
#11
Abhishek Dwivedi, R Ravi Kumar, Ajay Sharma, S K Pannu
INTRODUCTION: Stem cell transplantation is today's procedure of choice for management of various hematopoietic malignant and severe immunogenic disorders. High Resolution Computed Tomography (HRCT) is a common technique for the diagnosis of pulmonary complications in stem cell transplant recipients. There are a large number of complications which can complicate the post-transplant period. AIM: To study the role of HRCT chest in stem cell transplant patients developing pulmonary complications, detect any evidence of infection, detect clinical signs of lung infections, Graft versus Host Disease (GvHD) or other regimen related toxicities outlined earlier, detect any evidence of GvHD and correlate these clinical signs with radiological changes in the lungs...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28034505/high-performance-thin-layer-chromatography-mass-spectrometry-of-japanese-knotweed-flavan-3-ols-and-proanthocyanidins-on-silica-gel-plates
#12
Vesna Glavnik, Irena Vovk, Alen Albreht
On-line elution based TLC-MS is now a well-established technique, but the quality of the data obtained can sometimes be hampered by a severe spectral background or by strong ion suppression, especially when silica gel plates are used in combination with an acidic modifier in the developing solvent. We solved this issue simply and efficiently using two pre-developments of the plates, firstly with methanol-formic acid (10:1, v/v) and secondly with acetonitrile-methanol (2:1, v/v). This solution resulted in significant improvement in the sensitivity of HPTLC-MS methods...
December 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28028844/improved-tlc-bioautographic-assay-for-qualitative-and-quantitative-estimation-of-tyrosinase-inhibitors-in-natural-products
#13
Jinge Zhou, Qingjiu Tang, Tao Wu, Zhihong Cheng
INTRODUCTION: TLC bioautography for tyrosinase inhibitors has made recent progress; however, an assay with a relative low consumption of enzyme and quantitative capability would greatly advance the efficacy of related TLC bioautographic assays. OBJECTIVE: An improved TLC bioautographic assay for detecting tyrosinase inhibitors was developed and validated in this study. METHODS: L-DOPA (better water-solubility than L-tyrosine) was used as the substrate instead of reported L-tyrosine...
December 28, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28013553/in-vitro-anti-mrsa-activity-of-couroupita-guianensis-extract-and-its-component-tryptanthrin
#14
Danielle Cristina Machado Costa, Mariana Maria Barros de Azevedo, Davi Oliveira E Silva, Maria Teresa Villela Romanos, Thais Cristina Baeta Soares Souto-Padrón, Celuta Sales Alviano, Daniela Sales Alviano
Couroupita guianensis is known in Brazil as 'Abricó-de-Macaco' and it has some attributes such as: antihypertensive, analgesic and anti-inflammatory activities. This study evaluated the antimicrobial activity of ethanolic extract and fractions of C. guianensis flowers and isolation of bioactive component. These extracts and fractions were subjected to agar diffusion, MIC, TLC and bioautography to bacteria, filamentous fungi and yeasts. Among the fractions of EtOH extract, the DCM fraction was the most active, particularly against Methicillin-resistant Staphylococcus aureus (MRSA) with MIC of 156 μg/mL...
December 26, 2016: Natural Product Research
https://www.readbyqxmd.com/read/28009917/hierarchically-structured-photonic-crystals-for-integrated-chemical-separation-and-colorimetric-detection
#15
Qianqian Fu, Biting Zhu, Jianping Ge
A SiO2 colloidal photonic crystal film with a hierarchical porous structure is fabricated to demonstrate an integrated separation and colorimetric detection of chemical species for the first time. This new photonic crystal based thin layer chromatography process requires no dyeing, developing and UV irradiation compared to the traditional TLC. The assembling of mesoporous SiO2 particles via a supersaturation-induced-precipitation process forms uniform and hierarchical photonic crystals with micron-scale cracks and mesopores, which accelerate the diffusion of developers and intensify the adsorption/desorption between the analytes and silica for efficient separation...
December 23, 2016: Nanoscale
https://www.readbyqxmd.com/read/28005227/in-planta-production-and-characterization-of-a-hyperthermostable-gh10-xylanase-in-transgenic-sugarcane
#16
Jae Yoon Kim, Guang Nong, John D Rice, Maria Gallo, James F Preston, Fredy Altpeter
Sugarcane (Saccharum sp. hybrids) is one of the most efficient and sustainable feedstocks for commercial production of fuel ethanol. Recent efforts focus on the integration of first and second generation bioethanol conversion technologies for sugarcane to increase biofuel yields. This integrated process will utilize both the cell wall bound sugars of the abundant lignocellulosic sugarcane residues in addition to the sucrose from stem internodes. Enzymatic hydrolysis of lignocellulosic biomass into its component sugars requires significant amounts of cell wall degrading enzymes...
December 22, 2016: Plant Molecular Biology
https://www.readbyqxmd.com/read/28004216/pectinolytic-yeasts-from-cold-environments-novel-findings-of-guehomyces-pullulans-cystofilobasidium-infirmominiatum-and-cryptococcus-adeliensis-producing-pectinases
#17
Ivana Cavello, Agustín Albanesi, Dante Fratebianchi, Gabriela Garmedia, Silvana Vero, Sebastián Cavalitto
One hundred and three yeasts isolated from soil samples from King George Island and Tierra del Fuego province were screened in relation with their capability to produce pectinolytic enzymes. Although all the yeasts showed well-developed colonies at 20 °C, only eight showed a clear halo around the colony, indicative of pectin degradation. A secondary screening demonstrated that only four yeasts were capable to produce pectinases at low temperatures (8 °C). It could be seen that the selected yeasts were able to grow and produce high levels of polygalacturonase activity when submerged fermentations were performed using pectin-containing fruit wastes as substrates...
December 21, 2016: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28002918/the-correlation-between-preoperative-levels-of-albumin-and-tlc-and-mortality-in-patients-with-femoral-neck-fracture
#18
F Niccolai, P D Parchi, A Vigorito, G Pasqualetti, F Monzani, M Lisanti
A femoral neck fracture in an elderly patient often represents a major challenge for the orthopaedic surgeon who has to face not only the fracture, but also all the multiple issues related to age. Among others, malnutrition has been recognised as an important factor associated with severe aggravation in these patients. One-hundred-and-forty-seven patients were enrolled to investigate the use of two markers of patient nutritional status, i.e. serum albumin level and total leukocyte count (TLC), as predictors of mortality in the elderly patient suffering from proximal femur fracture...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/27993635/the-potential-of-aqueous-extracts-of-bellucia-dichotoma-cogn-melastomataceae-to-inhibit-the-biological-activities-of-bothrops-atrox-venom-a-comparison-of-specimens-collected-in-the-states-of-par%C3%A3-and-amazonas-brazil
#19
Valéria Mourão de Moura, Luana Yamille Andrade de Souza, Noranathan da Costa Guimarães, Ilia Gilmara Carvalho Dos Santos, Patrícia Danielle Oliveira de Almeida, Ricardo Bezerra de Oliveira, Rosa Helena Veras Mourão, Maria Cristina Dos-Santos
ETHNOPHARMACOLOGICAL IMPORTANCE: The effectiveness of aqueous extract of Bellucia dichotoma Cogn. (Melastomataceae) specimems collected in Santarém, PA, against some biological activities of Bothrops atrox venom (BaV) has been scientifically proven. Here, we analyzed the components and assessed the anti-snakebite potential of aqueous extracts of bark of B. dichotoma collected in Manaus, AM, (AEBd-MAO) and Santarém, PA, (AEBd-STM), both in Brazil. MATERIALS AND METHODS: The phytochemical profiles of the aqueous extracts were identified using thin layer chromatography (TLC), and the concentrations of phenolics were determined by colorimetric assay...
January 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27991764/high-troughput-lipidomic-and-transcriptomic-analysis-to-compare-sp2-0-cho-and-hek-293-mammalian-cell-lines
#20
Yue Zhang, Deniz Baycin Hizal, Amit Kumar, Joseph Priola, Michelle Bahri, Kelley M Heffner, Miao Wang, Xianlin Han, Michael A Bowen, Michael J Betenbaugh
A combined lipidomics and transcriptomics analysis was performed on mouse myeloma SP2/0, Chinese Hamster Ovary (CHO) and Human Embryonic Kidney (HEK) cells in order to compare widely used mammalian expression systems. Initial thin layer chromatography (TLC) analysis indicated phosphatidylethanolamine (PE) and phosphatidylcholine (PC) were the major lipid components with lower amounts of sphingomyelin (SM) in SP2/0 compared to CHO and HEK, which was subsequently confirmed and expounded upon following mass spectrometry (MS) analysis...
December 19, 2016: Analytical Chemistry
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