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https://www.readbyqxmd.com/read/28719813/comparative-stability-indicating-chromatographic-methods-for-determination-of-4-hexylresorcinol-in-pharmaceutical-formulation-and-shrimps
#1
Rafeek F Shokry, Lories I Bebawy, Mohamed R Elghobashy, Samah S Abbas
Three chromatographic stability-indicating methods were developed for determination of 4-hexylresorcinol in pure form and in a pharmaceutical formulation. Method A was based on a gradient elution liquid chromatographic HPLC determination of 4-hexylresorcinol, its related impurities and in presence of its degradation products. UPLC-MS/MS (Method B) was described for determination of the cited drug in presence of its degradation products. Method C was a thin- layer chromatography (TLC)-densitometry method for the separation and determination of the active ingredient, one of its related impurities and in presence of its degradation products...
July 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28718734/pullulan-production-from-synthetic-medium-by-a-new-mutant-of-aureobasidium-pullulans
#2
Guoqiang Chen, Jiming Wang, Yulong Su, Youshuang Zhu, Ge Zhang, Hongwei Zhao, Haobao Liu, Ying Yang, Rui Nian, Haibo Zhang, Yuxi Wei, Mo Xian
Pullulan with different molecular-weight could be applied in various fields. A UV-induced mutagenesis Aureobasidium pullulans UVMU6-1 was obtained from the strain A. pullulans CGMCC3.933 for the production of low-molecular-weight pullulan. Firstly, the obtained polysaccharide from A. pullulans UVMU6-1 was purified and identified to be pullulan with TLC, FT-IR and NMR. Then, culture medium and conditions for this strain were optimized by flask fermentation. Based on the optimized medium and culture conditions (pH 4, addition of 4 g/L Tween 80 for 96 h of cultivation), continuously fermentation was performed...
July 18, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28717290/to-compare-the-efficacy-of-c-reactive-protein-and-total-leucocyte-count-as-markers-for-monitoring-the-course-of-odontogenic-space-infections
#3
Rishi Bali, Parveen Sharma, Priya Ghanghas, Niti Gupta, Jay Dutt Tiwari, Abhiroop Singh, Nitin Sapra, Disha Goyal
OBJECTIVE: To compare the efficacy of CRP and TLC as markers for monitoring the course of odontogenic space infections (OSI) in 50 patients. METHODS: A Clinical severity scale (CSS) was developed to grade the severity of infections in patients. Blood samples were taken preoperatively and postoperatively at day 1, day 2, day 3 and day 7 for measuring the levels of CRP and TLC. The trends of CRP and TLC were analysed against the CSS. The data was subjected to paired "t" test, ANOVA, Spearman rank correlation, Pearson's bivariate correlation as appropriate...
September 2017: Journal of Maxillofacial and Oral Surgery
https://www.readbyqxmd.com/read/28716028/antiplasmodial-potential-and-quantification-of-aloin-and-aloe-emodin-in-aloe-vera-collected-from-different-climatic-regions-of-india
#4
Sandeep Kumar, Manila Yadav, Amita Yadav, Pooja Rohilla, Jaya Parkash Yadav
BACKGROUND: In this study, Aloe vera samples were collected from different climatic regions of India. Quantitative HPTLC (high performance thin layer chromatography) analysis of important anthraquinones aloin and aloe-emodin and antiplasmodial activity of crude aqueous extracts was done to estimate the effects of these constituents on antiplasmodial potential of the plant. METHODS: HPTLC system equipped with a sample applicator Linomat V with CAMAG sample syringe, twin rough plate development chamber (20 x 10 cm), TLC Scanner 3 and integration software WINCATS 1...
July 17, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28712956/refolding-of-a-novel-cholesterol-oxidase-from-pimelobacter-simplex-reveals-dehydrogenation-activity
#5
Hui-Min Qin, Jian-Wen Wang, Qianqian Guo, Songtao Li, Panpan Xu, Zhangliang Zhu, Dengyue Sun, Fuping Lu
Cholesterol oxidases, which catalyze the degradation of cholesterol to cholest-4-en-3-one, are widely used in the pharmaceutical and food processing industries. The cholesterol oxidase from Pimelobacter simplex (PsChO3) was transformed into E. coli BL21(DE3), but it was expressed mainly as inclusion bodies, and any soluble PsChO3 failed to bind to Ni-NTA resin. To overcome this obstacle, we devised a simple yet efficient purification and refolding process using 8 M urea for the solubilization of PsChO3 and achieved a high yield of the enzyme in its active form...
July 13, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28710640/analysis-of-intermediates-of-steroid-transformations-in-resting-cells-by-thin-layer-chromatography-tlc
#6
Govinda Guevara, Julián Perera, Juana-María Navarro-Llorens
Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28707542/the-physiological-consequences-of-different-distributions-of-diffuse-pleural-thickening-on-ct-imaging
#7
Duneesha de Fonseka, Anthony Edey, Louise Stadon, Jason Viner, Michael Darby, Nick A Maskell
OBJECTIVES: Diffuse pleural thickening refers to extensive visceral pleural fibrosis, with adhesion formation to the parietal pleura, obliterating the pleural space. The radiological definition of DPT remains controversial with most literature requiring the presence of an obliterated costophrenic angle for defining DPT. We conducted a study to investigate the variable distributions of DPT and associated lung function deficit. METHODS: Eighty-five patients referred to a pleural clinic with suspected pleural thickening were screened for our study...
July 14, 2017: British Journal of Radiology
https://www.readbyqxmd.com/read/28705995/reproducibility-of-airway-luminal-size-in-asthma-measured-by-hrct
#8
Robert H Brown, Robert J Henderson, Elizabeth A Sugar, Janet T Holbrook, Robert A Wise
High resolution computed tomography (HRCT) is a well-established imaging technology to measure lung and airway morphology in vivo. However, there is a surprising lack of studies examining HRCT reproducibility. The CPAP trial was a multicenter, randomized, 3-parallel arm, sham-controlled 12 week clinical trial to assess the use of a nocturnal CPAP device on airways reactivity to methacholine. The lack of a treatment effect of CPAP on clinical or HRCT measures provided an opportunity for the current analysis...
July 13, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28705748/effect-of-secondary-metabolite-of-actinidia-deliciosa-on-the-biofilm-and-extra-cellular-matrix-components-of-acinetobacter-baumannii
#9
Vishvanath Tiwari, Deepika Tiwari, Varsha Patel, Monalisa Tiwari
Acinetobacter baumannii, opportunistic nosocomial pathogen, increases gradually in the clinical setup. The high level of resistance mechanisms acquired by these bacteria makes their eradication difficult and biofilm formation is one of them. Biofilm comprises of closely packed bacterial population crowded together by extra-cellular matrix (ECM). ECM contains bacterial secreted polymers such as exopolysaccharides (EPS), proteins and extracellular-DNA (e-DNA) and rarely amyloidogenic proteins. Biofilm offers protection of underlying bacterial population against chemotherapeutic agents and host immune system...
July 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28704156/quality-of-life-in-patients-with-leg-ulcers-results-from-challenge-a-double-blind-randomised-controlled-trial
#10
S Meaume, A Dompmartin, C Lok, I Lazareth, M Sigal, F Truchetet, A Sauvadet, S Bohbot
OBJECTIVE: We recently showed the superiority of a matrix metalloproteinase (MMP) modulating dressing (foam impregnated with NOSF, nano-oligosaccharide factor) compared with a lipidocolloid matrix (TLC) control dressing in median wound area reduction (WAR). Here we report the results from the same study assessing the performance and safety of TLC-NOSF in the local management of venous leg ulcers (VLUs) or mixed leg ulcers and determining its impact on the patient's health-related quality of life (HRQoL)...
July 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/28704094/takotsubo-like-cardiomyopathy-in-a-large-cohort-of-patients-with-pheochromocytoma-and-paraganglioma
#11
Nadia Gagnon, Samer Mansour, Yoel Bitton, Isabelle Bourdeau
Pheochromocytoma (PHEO) and paraganglioma (PGL) (PPGL) may cause acute Takotsubo-like catecholamine cardiomyopathy (TLC). OBJECTIVE: To determine the prevalence and clinical presentation of TLC in a large cohort of patients with PPGL. METHODS: We reviewed retrospectively the records of consecutive patients with PPGL investigated in our center from 1995 to 2016. We collected clinical and paraclinical data of patients that had TLC in this cohort. We performed a literature review of cases of TTC-related to PPGL described previously from 1990-2015...
July 13, 2017: Endocrine Practice
https://www.readbyqxmd.com/read/28702094/disorders-of-nutritional-status-in-sepsis-facts-and-myths
#12
REVIEW
Katarzyna Kosałka, Ewelina Wachowska, Robert Słotwiński
The problem of diagnosing nutritional status disorders in septic patients remains unresolved. This is associated with the necessity of the introduction of newer and newer methods of assessing nutritional status, often requiring precise and expensive equipment as well as employment of professionals in this field in hospital wards, primarily including intensive care units (ICU). Methods that have been applied thus far for assessing nutritional status, also used in severely ill septic patients, have little impact on improving treatment results...
2017: Przegla̜d Gastroenterologiczny
https://www.readbyqxmd.com/read/28699723/fermentation-isolation-purification-and-characterization-of-an-antitubercular-antibiotic-from-streptomyces-luridus-mtcc-4402
#13
S Anuradha, K Shravan Kumar, S Bhama, V Kishan
Tuberculosis, caused by Mycobacterium tuberculosis, continues to be a serious public health problem around the world, and it urges the need for development of new antitubercular drugs. An antibiotic producing strain, Streptomyces luridus (MTCC 4402) was earlier isolated from soil by our group. In this work, the phylogenic status was established by 16S rRNA gene sequence analysis. The strain was found to be active against clinically resistant strains. The culture was grown in shake flasks in a medium containing cornsteep liquor, glucose, CaCO(3), soyabean meal and starch...
September 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/28699237/evaluation-of-visual-and-computer-based-ct-analysis-for-the-identification-of-functional-patterns-of-obstruction-and-restriction-in-hypersensitivity-pneumonitis
#14
Joseph Jacob, Brian J Bartholmai, Anne Laure Brun, Ryoko Egashira, Srinivasan Rajagopalan, Ronald Karwoski, Vasileios Kouranos, Maria Kokosi, David M Hansell, Athol U Wells
BACKGROUND AND OBJECTIVE: To determine whether computer-based quantification (CALIPER software) is superior to visual computed tomography (CT) scoring in the identification of CT patterns indicative of restrictive and obstructive functional indices in hypersensitivity pneumonitis (HP). METHODS: A total of 135 consecutive HP patients had CT parenchymal patterns evaluated quantitatively by both visual scoring and CALIPER. Results were evaluated against: forced vital capacity (FVC), total lung capacity (TLC), diffusing capacity for carbon monoxide (DLCO ) and a composite physiological index (CPI) to identify which CT scoring method better correlated with functional indices...
July 11, 2017: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/28698266/spirometry-static-lung-volumes-and-diffusing-capacity
#15
Carlos A Vaz Fragoso, Hilary C Cain, Richard Casaburi, Patty J Lee, Lynne Iannone, Linda S Leo-Summers, Peter H Van Ness
BACKGROUND: Spirometric Z-scores from the Global Lung Initiative (GLI) rigorously account for age-related changes in lung function and are thus age-appropriate when establishing spirometric impairments, including a restrictive pattern and air-flow obstruction. However, GLI-defined spirometric impairments have not yet been evaluated regarding associations with static lung volumes (total lung capacity [TLC], functional residual capacity [FRC], and residual volume [RV]) and gas exchange (diffusing capacity)...
July 11, 2017: Respiratory Care
https://www.readbyqxmd.com/read/28696523/nematic-dna-thermotropic-liquid-crystals-with-photoresponsive-mechanical-properties
#16
Lei Zhang, Sourav Maity, Kai Liu, Qing Liu, Robert Göstl, Giuseppe Portale, Wouter H Roos, Andreas Herrmann
Over the last decades, water-based lyotropic liquid crystals of nucleic acids have been extensively investigated because of their important role in biology. Alongside, solvent-free thermotropic liquid crystals (TLCs) from DNA are gaining great interest, owing to their relevance to DNA-inspired optoelectronic applications. Up to now, however, only the smectic phase of DNA TLCs has been reported. The development of new mesophases including nematic, hexagonal, and cubic structures for DNA TLCs remains a significant challenge, which thus limits their technological applications considerably...
July 11, 2017: Small
https://www.readbyqxmd.com/read/28693531/warm-needling-acupuncture-and-medicinal-cake-separated-moxibustion-for-hyperlipidemia-study-protocol-for-a-randomized-controlled-trial
#17
Mailan Liu, Qian Zhang, Shan Jiang, Mi Liu, Guoshan Zhang, Zenghui Yue, Qin Chen, Jie Zhou, Yifan Zou, Dan Li, Mingzhu Ma, Guobin Dai, Huan Zhong, Zhihong Wang, Xiaorong Chang
BACKGROUND: Acupuncture and moxibustion has been widely applied to hyperlipidemia treatment in clinical practice in China, serving as an alternative treatment to statins. Warm-needling acupuncture and medicinal cake-separated moxibustion have been separately reported with potential therapeutic effects on hyperlipidemia treatment in several studies but with limitations in study methodology. Combining these two modalities may provide a more advantageous strategy in treating hyperlipidemia...
July 10, 2017: Trials
https://www.readbyqxmd.com/read/28691448/evaluation-of-chemical-physical-properties-and-cytocompatibility-of-theracal-lc
#18
A Buonavoglia, D Lauritano, D Perrone, F Ardito, G Troiano, M Dioguardi, V Candotto, F J Silvestre, L Lo Muzio
TheraCal LC (TLC, Bisco Inc., Schaumburg, IL, USA) is a light-cured, resin-modified, calcium silicate-filled base/liner material designed for direct and indirect pulp-capping. In this study the result of the evaluation in vitro of the biocompatibility and chemical-physical properties of TLC are reported. TLC specimens were prepared under aseptic conditions in strict compliance with the manufacturer’s instructions and sterilized. Osteoblast-like cells (MG63) were used. For different time points, solubility, water uptake, alkalinizing activity and cytotoxicity were evaluated...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28688345/the-use-of-unburned-propellant-powder-for-shooting-distance-determination-part-ii-diphenylamine-reaction
#19
Rolf Hofer, Philipp Wyss
Shooting samples were produced on standard textile pats of six different ammunition types: four ammunitions with exclusively infrared luminescent propellant powder particles, one containing a mixture of luminescent and non-luminescent particles and one with only non-luminescent particles. Unburned propellant powder particles in the gunshot residue (GSR) on the textile of each sample were transferred onto TLC-plates with the aid of an organic solvent. The patterns of the partially and the all-luminescent propellant powder residue on the TLC-plates were visualized in the near infrared wavelength range by the aid of an IR-sensitive camera...
June 27, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28688028/novel-assessment-of-interstitial-lung-disease-using-the-computer-aided-lung-informatics-for-pathology-evaluation-and-rating-caliper-software-system-in-idiopathic-inflammatory-myopathies
#20
Patompong Ungprasert, Katelynn M Wilton, Floranne C Ernste, Sanjay Kalra, Cynthia S Crowson, Srinivasan Rajagopalan, Brian J Bartholmai
PURPOSE: To evaluate the correlation between measurements from quantitative thoracic high-resolution CT (HRCT) analysis with "Computer-Aided Lung Informatics for Pathology Evaluation and Rating" (CALIPER) software and measurements from pulmonary function tests (PFTs) in patients with idiopathic inflammatory myopathies (IIM)-associated interstitial lung disease (ILD). METHODS: A cohort of patients with IIM-associated ILD seen at Mayo Clinic was identified from medical record review...
July 7, 2017: Lung
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